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7 results on '"Simon, Myriam"'

1. Characteristics and impact of infiltration of B-cells from systemic sclerosis patients in a 3D healthy skin model.

Author

Le Maître, Mathilde, Guerrier, Thomas, Collet, Aurore, Derhourhi, Mehdi, Meneboo, Jean-Pascal, Toussaint, Bénédicte, Bonnefond, Amélie, Villenet, Céline, Sebda, Shéhérazade, Bongiovanni, Antonino, Tardivel, Meryem, Simon, Myriam, Jendoubi, Manel, Daunou, Blanche, Largy, Alexis, Figeac, Martin, Dubucquoi, Sylvain, and Launay, David

Subjects
IMMUNOGLOBULIN genes, B cells, EXTRACELLULAR matrix, GENETIC overexpression, SYSTEMIC scleroderma
Abstract

Introduction: In systemic sclerosis (SSc), B-cells are activated and present in the skin and lung of patients where they can interact with fibroblasts. The precise impact and mechanisms of the interaction of B-cells and fibroblasts at the tissular level are poorly studied. Objective: We investigated the impact and mechanisms of B-cell/fibroblast interactions in cocultures between B-cells from patients with SSc and 3-dimensional reconstituted healthy skin model including fibroblasts, keratinocytes and extracellular matrix. Methods: The quantification and description of the B-cell infiltration in 3D cocultures were performed using cells imagery strategy and cytometry. The effect of coculture on the transcriptome of B-cells and fibroblasts was studied with bulk and single-cell RNA sequencing approaches. The mechanisms of this interaction were studied by blocking key cytokines like IL-6 and TNF. Results: We showed a significant infiltration of B-cells in the 3D healthy skin model. The amount but not the depth of infiltration was higher with B-cells from SSc patients and with activated B-cells. B-cell infiltrates were mainly composed of naïve and memory cells, whose frequencies differed depending on B-cells origin and activation state: infiltrated B-cells from patients with SSc showed an activated profile and an overexpression of immunoglobulin genes compared to circulating B-cells before infiltration. Our study has shown for the first time that activated B-cells modified the transcriptomic profile of both healthy and SSc fibroblasts, toward a pro-inflammatory (TNF and IL-17 signaling) and interferon profile, with a key role of the TNF pathway. Conclusion: B-cells and 3D skin cocultures allowed the modelization of B-cells infiltration in tissues observed in SSc, uncovering an influence of the underlying disease and the activation state of B-cells. We showed a pro-inflammatory effect on skin fibroblasts and pro-activation effect on infiltrating B-cells during coculture. This reinforces the role of B-cells in SSc and provide potential targets for future therapeutic approach in this disease. [ABSTRACT FROM AUTHOR]

Published
2024
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4. Prevalence and Clinical Associations of Antiphospholipid Antibodies in Systemic Sclerosis: New Data From a French Cross-Sectional Study, Systematic Review, and Meta-Analysis

Author

Sobanski, Vincent, Lemaire-Olivier, Angélique, Giovannelli, Jonathan, Dauchet, Luc, Simon, Myriam, Lopez, Benjamin, Yelnik, Cécile, Lambert, Marc, Hatron, Pierre-Yves, Hachulla, Eric, Dubucquoi, Sylvain, and Launay, David

Subjects
Male, Venous Thrombosis, Scleroderma, Systemic, systemic sclerosis, Immunology, miscarriage, antiphospholipid antibodies, Middle Aged, Antiphospholipid Syndrome, Abortion, Spontaneous, Cross-Sectional Studies, immune system diseases, Pregnancy, beta 2-Glycoprotein I, Antibodies, Anticardiolipin, Lupus Coagulation Inhibitor, pulmonary hypertension, Antibodies, Antiphospholipid, Humans, Female, France, skin and connective tissue diseases, neoplasms, Original Research, Aged
Abstract

Objectives: Antiphospholipid antibodies (aPL) can be present in the sera of systemic sclerosis (SSc) patients. This study aimed to determine the prevalence of aPL in a cross-sectional study of SSc patients, to assess their clinical associations, to perform a systematic review of published reports and a meta-analysis to estimate the worldwide prevalence of aPL in SSc. Methods: Two-hundred and forty-nine SSc patients were consecutively tested once for lupus anticoagulant (LA), anticardiolipin (aCL), and anti-β2glycoprotein I (anti-β2GpI) antibodies. Clinical associations with aPL positivity were studied using a logistic regression model. A systematic review of the literature was carried out in PubMed and Embase. Meta-analysis was performed using number of aPL positive (at least one of the three antibodies positive) and negative patients. Meta-regression was used to study potential factors explaining the heterogeneity between studies. Results: In our cross-sectional study, aPL positivity was found in 16 patients (prevalence 6.4%; 95%CI [3.8–10.4]). In multivariate analysis, there was a significant association between aPL positivity and venous thrombosis (VT) (OR 6.25 [1.18–33.00]; p = 0.028) and miscarriage (OR 5.43; 95%CI [1.31–22.13]; p = 0.017). Twenty-four studies were included in the meta-analysis, representing a total population of 3036 SSc patients. The overall pooled prevalence of aPL in SSc was 14% (9–20) with a high degree of heterogeneity among studies. Conclusion: This study found a prevalence of aPL positivity in our SSc population of 6.4% (3.8–10.4) and an overall worldwide pooled prevalence of 14% (9–20). In our SSc population, aPL positivity was associated with VT and miscarriage. These data provide additional insights into the role of aPL in the vasculopathy observed in SSc.

Published
2018

5. Gestion immobilière

Author

Berre, Stéphane, Camoz, Jean-Yves, Cohet, Frédérique, Kaddouch, Martine, Simon, Myriam, Université Jean Moulin Lyon 3 - Faculté de Droit (UJML3 Droit), Université Jean Moulin - Lyon 3 (UJML), Université de Lyon-Université de Lyon, Centre de Recherches Juridiques (CRJ), Université Pierre Mendès France - Grenoble 2 (UPMF), and COHET, FREDERIQUE

Subjects
[SHS.DROIT]Humanities and Social Sciences/Law, [SHS.DROIT] Humanities and Social Sciences/Law
Abstract

International audience; BTS Professions immobilières DUT Immobilier Licences professionnelles Métiers de l'immobilierCet ouvrage destiné prioritairement aux étudiants en BTS professions immobilière, DUT immobilier, et licence professionnelle métiers de l'immobilier a pour objectif de rappeler les fondamentaux dans le domaine de la gestion immobilière. Cet ouvrage est à jour des dispositions législatives et réglementaires au 1er août 2015.Grâce à leurs compétences diversifiées et complémentaires, les auteurs ont à la fois développé l'essentiel des connaissances nécessaires en transaction immobilière et les pratiques professionnelles associées.

Published
2015

6. Transaction immobilière

Author

Berre, Stéphane, Camoz, Jean-Yves, Cohet, Frédérique, Kaddouch, Martine, Simon, Myriam, Université Jean Moulin Lyon 3 - Faculté de Droit (UJML3 Droit), Université Jean Moulin - Lyon 3 (UJML), Université de Lyon-Université de Lyon, Centre de Recherches Juridiques (CRJ), Université Pierre Mendès France - Grenoble 2 (UPMF), and COHET, FREDERIQUE

Subjects
Droit, [SHS.DROIT]Humanities and Social Sciences/Law, [SHS.DROIT] Humanities and Social Sciences/Law, Immobilier, Obligations (droit), France
Abstract

International audience; Cet ouvrage destiné prioritairement aux étudiants en BTS professions immobilière, DUT immobilier, et licence professionnelle métiers de l'immobilier a pour objectif de rappeler les fondamentaux dans le domaine de la transaction immobilière. Cet ouvrage est à jour des dispositions législatives et réglementaires au 1er août 2015.Grâce à leurs compétences diversifiées et complémentaires, les auteurs ont à la fois développé l'essentiel des connaissances nécessaires en transaction immobilière et les pratiques professionnelles associées.

Published
2015

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