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INTRODUCTION: Proton pump inhibitors (PPIs) are potent and effective acid suppressant agents. There are emerging concerns that PPI overuse leads to escalating healthcare costs, drug-drug interactions, and adverse clinical outcomes. Exploring the appropriateness of PPI therapy among hospitalised patients in Malaysia is crucial. Thus, this study aims to determine the appropriateness of proton pump inhibitors utilisation in Sultan Ismail Hospital, Malaysia. MATERIALS AND METHODS: This single-center retrospective cross-sectional study was conducted at a public tertiary hospital situated in the Johore state of Malaysia. Patients recruited were 12 years and above who had received PPI in January 2017. Medical information was reviewed from electronic medical records. Patients were classified into three subgroups based on indications adopted from evidencebased guidelines. RESULTS: 422 patients were enrolled, 47.4% (n=200) fulfilled the Food Drug Administration (FDA) approved indications, 25.4% (n=107) had borderline indications, and 27.2% (n=115) had inappropriate PPI usage. Improper PPI therapy was observed with a similar fraction of patients in medical (24.5%, n= 58 ) and non-medical disciplines (30.8 %, n=57). Stress ulcer prevention in low-risk subjects and individuals with no apparent indication were reasons for PPI overuse. CONCLUSION: PPI overuse is prevailing in hospitalised adult patients. Promoting the awareness of guideline-based indications and adverse clinical events of PPI among clinicians is imperative in improving PPI prescribing. [ABSTRACT FROM AUTHOR]

Advancements in nanotechnology led to significant improvements in synthesizing plasmon-enhanced nanoarchitectures for biosensor applications, and high-yield productivity at low cost is vital to step further into medical commerce. Metal nanoframes via wet chemistry are gaining attention for their homogeneous structure and outstanding catalytic and optical properties. However, nanoframe morphology should be considered delicately when brought to biosensors to utilize its superior characteristics thoroughly, and the need to prove its clinical applicability still remains. Herein, we controlled the frameworks of double-walled nanoframes (DWFs) precisely via wet chemistry to construct a homogeneous plasmon-enhanced nanotransducer for localized surface plasmon resonance biosensors. By tuning the physical properties considering the finite-difference time-domain simulation results, biomolecular interactions were feasible in the electromagnetic field-enhanced nanospace. As a result, DWF 10 exhibited a 10-fold lower detection limit of 2.21 fM compared to DWF 14 for tau detection. Further application into blood-based clinical and Alzheimer's disease (AD) diagnostics, notable improvement in classifying mild cognitive impairment patients against healthy controls and AD patients, was demonstrated along with impressive AUC values. Thus, in response to diverse detection methods, optimizing nanoframe dimensions such as nanogap and frame thickness to maximize sensor performance is critical to realize future POCT diagnosis.

Although biocides are important materials in modern society and help protect human health and the environment, increasing exposure to combined biocides can cause severe side effects in the human body, such as lung fibrosis. In this study, we developed a receptonics system to screen for biocides in combined household chemical products based on biocides. The system contains transient receptor potential ankyrin 1 (TRPA1) nanovesicles (NVs) to sense biocides based on pain receptors and a side-gated field-effect transistor (SGFET) using a single-layer graphene (SLG) micropattern channel. The binding affinities between the TRPA1 receptor and the various biocides were estimated by performing biosimulation and using a calcium ion (Ca 2+ ) assay, and the sensitivity of the system was compared with that of TRPA1 NV receptonics systems. Based on the results of the TRPA1 NV receptonics system, the antagonistic and potentiation effects of combined biocides and household chemical products depended on the concentration. Finally, the TRPA1 NV receptonics system was applied to screen for biocides in real products, and its performance was successful. Based on these results, the TRPA1 NV receptonics system can be utilized to perform risk evaluations and identify biocides in a simple and rapid manner., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Colorectal cancer (CRC) continues to demonstrate high incidence and mortality rates, emphasizing that implementing strategic measures for prevention and treatment is crucial. Recently, the dopamine receptor D2 (DRD2), a G protein-coupled receptor, has been reported to play multiple roles in growth of tumor cells. This study investigated the anticancer potential of domperidone, a dopamine receptor D2 antagonist, in HCT116 human CRC cells. Domperidone demonstrated concentration- and time-dependent reductions in cell viability, thereby inducing apoptosis. The molecular mechanism revealed that domperidone modulated the mitochondrial pathway, decreasing mitochondrial Bcl-2 levels, elevating cytosolic cytochrome C expression, and triggering caspase- 3, -7, and -9 cleavage. Domperidone decreased in formation of β-arrestin2/MEK complex, which contributing to inhibition of ERK activation. Additionally, treatment with domperidone diminished JAK2 and STAT3 activation. Treatment of U0126, the MEK inhibitor, resulted in reduced phosphorylation of MEK, ERK, and STAT3 without alteration of JAK2 activation, indicating that domperidone targeted both MEK-ERK-STAT3 and JAK2-STAT3 signaling pathways, respectively. Immunoblot analysis revealed that domperidone also downregulated DRD2 expression. Domperidone-induced reactive oxygen species (ROS) generation and N -acetylcysteine treatment mitigated ROS levels and restored cell viability. An in vivo xenograft study verified the significant antitumor effects of domperidone. These results emphasize the multifaceted anticancer effects of domperidone, highlighting its potential as a promising therapeutic agent for human CRC.

Ion channels, which are key to physiological regulation and drug discovery, control ion flux across membranes, and their dysregulation leads to various diseases. Ca 2+ monitoring is crucial for cellular signaling when performing Ca-based assays in ion channel research; these assays are widely utilized in both academic and pharmaceutical contexts for drug screening and pharmacological profiling. However, existing detection methods are limited by slow detection speeds, low throughput, complex processes, and low analyte viability. In this study, we developed a label-free optical biosensing method using a conical Au/polydimethylsiloxane platform tailored to detect Ca 2+ influx in A549-originated nanovesicles facilitated by the transient receptor potential ankyrin 1 (TRPA1) channel. Nanovesicles expressing cellular signaling components mimic TRPA1 signal transduction in cell membranes and improve analyte viability. The conical Au/polydimethylsiloxane sensor converted Ca 2+ influx events induced by specific agonist exposure into noticeable changes in relative transmittance under visible light. The optical transmittance change accompanying Ca 2+ influx resulted in an enhanced sensing response, high accuracy and reliability, and rapid detection (∼5 s) without immobilization or ligand treatments. In the underlying sensing mechanism, morphological variations in nanovesicles, which depend on Ca 2+ influx, induce a considerable light scattering change at an interface between the nanovesicle and Au, revealed by optical simulation. This study provides a foundation for developing biosensors based on light-matter interactions. These sensors are simple and cost-effective with superior performance and diverse functionality.

The outbreak of emerging infectious diseases gave rise to the demand for reliable point-of-care testing methods to diagnose and manage those diseases in early onset. However, the current on-site testing methods including lateral flow immunoassay (LFIA) suffer from the inaccurate diagnostic result due to the low sensitivity. Herein, we present the surface-enhanced Raman scattering-based lateral flow immunoassay (SERS-LFIA) by introducing phage-templated hierarchical plasmonic assembly (PHPA) nanoprobes to diagnose a contagious disease. The PHPA was fabricated using gold nanoparticles (AuNPs) assembled on bacteriophage MS2, where inter-particle gap sizes can be adjusted by pH-induced morphological alteration of MS2 coat proteins to provide the maximum SERS amplification efficiency via plasmon coupling. The plasmonic probes based on the PHPA produce strong and reproducible SERS signal that leads to sensitive and reliable diagnostic results in SERS-LFIA. The developed SERS-LFIA targeting severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) antibodies for a proof of concept had <100 pg/mL detection limits with high specificity in serum, proving it as an effective diagnostic device for the infectious diseases. Clinical validation using human serum samples further confirmed that the PHPA-based SERS-LFIA can distinguish the patients with COVID-19 from healthy controls with significant accuracy. These outcomes prove that the developed SERS-LFIA biosensor can be an alternative point-of-care testing (POCT) method against the emerging infectious diseases, in combination with the commercially available portable Raman devices., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The differential diagnosis between mild cognitive impairment (MCI) and Alzheimer's disease (AD) has been highly demanded for its effectiveness in preventing and contributing to early diagnosis of AD. To this end, we developed a single plasmonic asymmetric nanobridge (PAN)-based biosensor to differentially diagnose MCI and AD by quantitative profiling of phosphorylated tau proteins (p-tau) in clinical plasma samples, which revealed a significant correlation with AD development and progression. The PAN was designed to have a conductive junction and asymmetric structure, which was unable to be synthesized by the traditional thermodynamical methods. For its unique morphological characteristics, PAN features high electromagnetic field enhancement, enabling the biosensor to achieve high sensitivity, with a limit of detection in the attomolar regime for quantitative analysis of p-tau. By introducing support vector machine (SVM)-based machine learning algorithm, the improved diagnostic system was achieved for prediction of healthy controls, MCI, and AD groups with an accuracy of 94.47 % by detecting various p-tau species levels in human plasma. Thus, our proposed PAN-based plasmonic biosensor has a powerful potential in clinical utility for predicting the onset of AD progression in the asymptomatic phase., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

This study was performed to investigate the protective effects of Allium ochotense on fatty liver and hepatitis in chronic alcohol-induced hepatotoxicity. The physiological compounds of a mixture of aqueous and 60% ethanol (2:8, w / w ) extracts of A. ochotense (EA) were identified as kestose, raffinose, kaempferol and quercetin glucoside, and kaempferol di-glucoside by UPLC Q-TOF MS E . The EA regulated the levels of lipid metabolism-related biomarkers such as total cholesterol, triglyceride, low-density lipoprotein (LDL), and high-density lipoprotein (HDL)-cholesterol in serum. Also, EA ameliorated the levels of liver toxicity-related biomarkers such as glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), and total bilirubin in serum. EA improved the antioxidant system by reducing malondialdehyde contents and increasing superoxide dismutase (SOD) levels and reduced glutathione content. EA improved the alcohol metabolizing enzymes such as alcohol dehydrogenase, acetaldehyde dehydrogenase, and cytochrome P450 2E1 (CYP2E1). Treatment with EA alleviated lipid accumulation-related protein expression by improving phosphorylation of AMP-activated protein kinase (p-AMPK) expression levels. Especially, EA reduced inflammatory response by regulating the toll-like receptor-4/nuclear factor kappa-light-chain-enhancer of activated B cells (TLR-4/NF-κB) signaling pathway. EA showed an anti-apoptotic effect by regulating the expression levels of B-cell lymphoma 2 (BCl-2), BCl-2-associated X protein (BAX), and caspase 3. Treatment with EA also ameliorated liver fibrosis via inhibition of transforming growth factor-beta 1/suppressor of mothers against decapentaplegic (TGF-β1/Smad) pathway and alpha-smooth muscle actin (α-SMA). Therefore, these results suggest that EA might be a potential prophylactic agent for the treatment of alcoholic liver disease.

Bioplastic production using cyanobacteria can be an effective strategy to cope with environmental problems caused by using petroleum-based plastics. Synechococcus elongatus UTEX 2973 with heterogeneous phaCAB can produce bioplastic polyhydroxybutyrate (PHB) with a high CO 2 uptake rate. For cost-effective production of PHB in S. elongatus UTEX 2973, phaCAB was expressed by the constitutive P cpc560 , resulting in the production of 226 mg/L of PHB by only photoautotrophic cultivation without the addition of inducer. Several culture conditions were applied to increase PHB productivity, and when acetate was supplied at a concentration of 1 g/L as an organic carbon source, productivity significantly increased resulting in 607.2 mg/L of PHB and additive cost reduction of more than 300 times was achieved compared to IPTG. Consequently, these results suggest the possibility of cyanobacteria as an agent that can economically produce PHB and as a solution to the problem of petroleum-based plastics., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Background/objectives: Few studies have provided evidence of the association between diet quality and dental caries. This study aimed to examine the association between diet quality and untreated dental caries in a Korean representative population., Subjects/methods: The study population included a sample of 13,815 participants, aged ≥ 19 from the Korea National Health and Nutrition Examination Survey during 2013-2015. The explanatory variable was diet quality and the outcome variable was untreated dental caries. Untreated dental caries were defined by the number of decayed teeth recorded according to the criteria established by the World Health Organization. Diet quality was defined by using the Korean Healthy Eating Index (KHEI) through the 24-h recall methods. We assessed the association between diet quality and untreated dental caries while adjusting for age, sex, education, income, smoking status, dental visits, toothbrushing frequencies, obesity, and diabetes mellitus., Results: The mean overall KHEI scores in the untreated dental caries group were significantly lower than those in the group without untreated dental caries. Significant differences were observed in the untreated dental caries group based on the KHEI quartiles ( P < 0.001). After adjusting for potential confounders, the quartiles of KHEI scores showed an association with untreated dental caries, demonstrating a dose-effect trend (odds ratio [OR], 1.57; 95% confidence interval [CI], 1.35-1.84 for 1st quartile; OR, 1.38; 95% CI, 1.19-1.59 for 2nd quartile; OR, 1.32; 95% CI, 1.14-1.53 for 3rd quartile; reference quartile highest])., Conclusions: The findings indicated an inverse association between diet quality and untreated dental caries in Korean adults. Healthcare providers should take into account the significant role of diet quality in preventing and managing oral health., Competing Interests: Conflict of Interest: The authors declare no potential conflicts of interests., (©2023 The Korean Nutrition Society and the Korean Society of Community Nutrition.)

Serological detection of antibodies for diagnosing infectious diseases has advantages in facile diagnostic procedures, thereby contributing to controlling the spread of the pathogen, such as in the recent SARS-CoV-2 pandemic. Lateral flow immunoassay (LFIA) is a representative serological antibody detection method suitable for on-site applications but suffers from low clinical accuracy. To achieve a simple and rapid serological screening as well as the sensitive quantification of antibodies against SARS-CoV-2, a colorimetric and fluorescent dual-mode serological LFIA sensor incorporating metal-enhanced fluorescence (MEF) was developed. For the strong fluorescence signal amplification, fluorophore Cy3 was immobilized onto gold nanoparticles (AuNPs) with size-controllable spacer polyethyleneglycol (PEG) to maintain an optimal distance to induce MEF. The sensor detects the target IgG with a concentration as low as 1 ng mL -1 within 8 minutes. The employment of the MEF into the dual-mode serological LFIA sensor shows a 1000-fold sensitivity improvement compared with that of colorimetric LFIAs. The proposed serological LFIA sensor was tested with 73 clinical samples, showing sensitivity, specificity, and accuracy of 95%, 100%, and 97%, respectively. In conclusion, the dual-mode serological LFIA has great potential for application in diagnosis and an epidemiological survey of vaccine efficacy and immunity status of individuals., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

Observations indicate that groundwater-laden sedimentary aquifers are extensive beneath large portions of the Greenland and Antarctic ice sheets. A reduction in the mechanical loading of aquifers is known to lead to groundwater exfiltration, a discharge of groundwater from the aquifer. Here, we provide a simple expression predicting exfiltration rates under a thinning ice sheet. Using contemporary satellite altimetry observations, we predict that exfiltration rates may reach tens to hundreds of millimeters per year under the fastest thinning parts of the Antarctic Ice Sheet. In parts of West Antarctica, predicted rates of exfiltration would cause the total subglacial water discharge rate to be nearly double what is currently predicted from subglacial basal melting alone. Continued Antarctic Ice Sheet thinning into the future guarantees that the rate and potential importance of exfiltration will only continue to grow. Such an increase in warm, nutrient-laden subglacial water discharge would cause changes in ice sliding, melt of basal ice and marine biological communities.

Alzheimer's disease (AD) is a neurodegenerative disease of complex pathogenesis, with overt symptoms following disease progression. Early AD diagnosis is challenging due to the lack of robust biomarkers and limited patient access to diagnostics via neuroimaging and cerebrospinal fluid (CSF) tests. Exosomes present in body fluids are attracting attention as diagnostic biomarkers that directly reflect neuropathological features within the brain. In particular, exosomal miRNAs (exomiRs) signatures are involved in AD pathogenesis, showing a different expression between patients and the healthy controls (HCs). However, low yield and high homologous nature impede the accuracy and reproducibility of exosome blood-based AD diagnostics. Here, we developed a programmable curved plasmonic nanoarchitecture-based biosensor to analyze exomiRs in clinical serum samples for accurate AD diagnosis. To allow the detection of exomiRs in serum at attomolar levels, nanospaces (e.g., nanocrevice and nanocavity) were introduced into the nanostructures to dramatically increase the spectral sensitivity by adjusting the bending angle of the plasmonic nanostructure through sodium chloride concentration control. The developed biosensor classifies individuals into AD, mild cognitive impairment (MCI) patients, and HCs through profiling and quantifying exomiRs. Furthermore, integrating analysis expression patterns of multiple exosomal biomarkers improved serum-based diagnostic performance (average accuracy of 98.22%). Therefore, precise, highly sensitive serum-derived exosomal biomarker detection-based plasmonic biosensor has a robust capacity to predict the molecular pathologic of neurodegenerative disease, progression of cognitive decline, MCI/AD conversion, as well as early diagnosis and treatment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

This study evaluated economic feasibility through production efficiency, return on investment (ROI) and payout time of a hybrid system using a photobioreactor (PBR)-light guide panel (LGP)-PBR array (PLPA) and solar cells developed for astaxanthin and ω-3 FA simultaneous production of Haematococcus pluvialis. The economic feasibility of the PLPA hybrid system (8 PBRs) and the PBR-PBR-PBR array (PPPA) system (8 PBRs) was evaluated for producing high-value products while effectively reducing CO 2 . Introducing a PLPA hybrid system has increased the amount of culture per area by 1.6 times. Also, the shading effect was effectively suppressed with an LGP placed between each PBR, increasing biomass and astaxanthin productivity by 3.39-fold and 4.79-fold, respectively compared to the untreated H. pluvialis cultures. In addition, ROI increased by 6.55 and 4.71 times, and the payout time was reduced by 1.34 and 1.37 times, respectively in 10 and 100-ton scale processes., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Wastewater treatment plants are indispensable facilities, which emit a massive amount of greenhouse gases. To boost CO 2 mitigation and wastewater treatment performance, mixotrophic microalgae cultivation using wastewater has recently been proposed. In this study, food industry wastewater (waste soy sauce) was applied to Chlorella sorokiniana UTEX 2714 cultivation. By using a medium with 20% (v/v) of 10-fold diluted soy sauce, the biomass and fatty acid methyl ester (FAME) productivity enhanced by 1.93 and 1.76 times, respectively. Biomass productivity increased up to 5.2 times when using medium with high soy sauce content under high-intensity light that inhibits cell growth in photoautotrophic environments. Furthermore, industrial flue gas treatment with wastewater was demonstrated by outdoor semi-continuous cultivation with 42% improved biomass production. Consequently, these results suggest that mixotrophic microalgal cultivation has great potential to address both climate change and water pollution while producing valuable products and can contribute to building a sustainable society., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

This study was conducted to increase the productivity of biomass that contains high astaxanthin content by developing a mutant Haematococcus pluvialis strain with strong environmental tolerance. H. pluvialis has a low cell-growth rate and is vulnerable to stressors such as salinity or light intensity, which may hinder large-scale commercial cultivation. A mutant M5 strain selected through 5000-Gy gamma irradiation showed improved biomass and astaxanthin production under high-salinity and high-light intensity conditions. With enhanced SOD activity and overexpressed astaxanthin biosynthesis genes (lyc, crtR-b, bkt2), M5 demonstrated an increase in biomass and astaxanthin productivity by 86.70 % and 66.15 %, respectively compared to those of untreated cells. Also, the omega-3 content of M5 increased by 149.44 % under 40 mM CaCl 2 compared to the untreated cells. Finally, even when subjected to high-intensity light irradiation for the whole life cycle, the biomass and astaxanthin concentration increased by 84.99 % and 241 %, respectively, compared to the wild-type cells., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Background: Air pollutants can influence local and systemic inflammation, oxidative stress and microbiome composition. Therefore, air pollution may potentially represent an unexplored modifiable risk indicator for periodontitis. The aim of the current cross-sectional study was to investigate the epidemiological association between outdoor air pollution and periodontitis in a representative sample of the South Korean population., Methods: A total of 42,020 individuals, which were representative of 35.2 million South Koreans, were examined. The mean annual levels of particulate matter of 10 μm (PM10), ozone, sulfur dioxide (SO 2 ), nitrogen dioxide (NO 2 ) and humidity, were studied. Periodontitis was defined according to the Community Periodontal Index (CPI ≥ 3). Simple and multiple regression analyses using four different models were applied., Results: Every 5-μg/m 3 increase in PM10 (OR = 1.17; 95% confidence interval-CI: 1.11-1.24) and of 0.005 ppm in ozone levels (OR = 1.4; 95% CI: 1.00-1.30) were positively associated with periodontitis prevalence. Conversely, every 5% increase in humidity (OR = 0.94; 95% CI: 0.90-0.99) and 0.003 ppm increase in NO 2 levels (OR = 0.93; 95% CI: 0.89-0.96) were inversely associated with periodontitis occurrence., Conclusions: In this nationally representative population several air pollutants were found to be associated with periodontitis occurrence. Hence, the present results suggest that air pollution may be a new modifiable risk indicator for periodontitis.

The culture method using sodium acetate and glucose, widely used as organic carbon sources in the mixotrophy of Haematococcus pluvialis, was compared with its autotrophy. In the 12-day culture, mixotrophy using sodium acetate and glucose increased by 40.4% and 77.1%, respectively, compared to autotrophy, but the mechanisms for the increasing biomass were different. The analysis of the mechanism was divided into autotrophic and heterotrophic metabolism. The mixotrophy with glucose increased the biomass by directly supplying the substrate and ATP to the TCA cycle while inhibiting photosynthesis. Gene expressions related to glycolysis and carbon fixation pathway were confirmed in autotrophy and mixotrophy with glucose and acetate. The metabolism predicted in the mixotrophy with acetate and glucose was proposed via autotrophic and heterotrophic metabolism analysis. The mechanism of Haematococcus pluvialis under mixotrophic conditions with high CO 2 concentration was confirmed through this study., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Hydrogen as a clean fuel is receiving attention because it generates only water and a small amount of nitrogen oxide upon combustion. Biohydrogen production using microalgae is considered to be a highly promising carbon-neutral technology because it can secure renewable energy while efficiently reducing CO 2 emissions. However, previous studies have mainly focused on improving the biological performance of microalgae; these approaches have struggled to achieve breakthroughs in commercialization because they do not heavily consider the complexity of the entire production process with microalgae, including large-scale cultivation, biomass harvest, and biomass storage. This work presents an in-depth analysis of the state-of-the-art technologies focused on large-scale cultivation systems with efficient downstream processes. Considering the individual processes of biohydrogen production, strategies are discussed to minimize carbon emissions and improve productivity simultaneously. A comprehensive understanding of microalgae-derived biohydrogen production suggests future directions for realizing environmental and economic sustainability., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

In this study, the effects of microalgae-based biomineralization in a semi-continuous process (M-BSP) on biomass productivity and CO 2 fixation rate were investigated. M-BSP significantly improved biomass production and CO 2 fixation rate at the second stage of induction by sustaining relatively high photosynthetic rate without exposure to toxic substances (e.g., chlorellin) from aging cells using the microalgae Chlorella HS2. In conventional systems, cells do not receive irradiated light evenly, and many cells age and burst because of the long culture period. In contrast, in the M-BSP, the photosynthesis efficiency increases and biomass production is not inhibited because most of the cells can be harvested during shorter culture period. The accumulated biomass production and CO 2 fixation rate of the HS2 cells cultured under M-BSP increased by 4.67- (25 ± 1.09 g/L) and 10.9-fold (30.29 ± 1.79 g/L day -1 ), respectively, compared to those cultured without the CaCl 2 treatment., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Photosynthetic polyhydroxybutyrate (PHB) production is an attractive technology for realizing a sustainable society by simultaneously producing useful biodegradable plastics and mitigating CO 2 . It is necessary to establish an economical large-scale photobioreactor (PBR) capable of effectively cultivating photosynthetic microorganisms such as cyanobacteria. A roll-to-roll winding machine/heat-sealer hybrid system for fabricating an easy-to-scale-up PBR was developed in the present study. The baffle design was optimized to facilitate mass transfer within the PBR, and the operating conditions of the gas sparger were investigated to maximize the CO 2 transfer efficiency. The newly developed PBR was able to produce biomass of PHB content 10.7 w/w% at a rate of 6.861 g m -2 d -1 , 21 % improved biomass productivity compared with the existing PBR. It was confirmed that biomass productivity was maintained even when PBR was scaled up to 2 tons. Consequently, the newly developed PBR is expected to improve the feasibility of photosynthetic PHB production., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

The microalgae-based bioconversion process is a promising carbon utilization technology because it can upgrade CO 2 into valuable substances, but a multiplex monitoring system required for process control to maximize biomass productivity has not been well established. Herein, a 3D printed real-time optical density monitoring device (RTOMD) combined platform was presented. This platform enables precise kinetics analysis by maintaining high accuracy (over 95 %) under raucous outdoor conditions. Through RTOMD-based high-frequency measurements, it was observed that maximum biomass productivity of 4.497 g L -1  d -1 was reached, which greatly exceeds the requirements for a feasible microalgae process. We discovered that the CO 2 fixation efficiency could be achieved to 70.75 %, indicating the potential of a bioconversion process to realize a carbon-neutral society. Consequently, the RTOMD system can contribute to promoting microalgae cultivation as an attractive carbon mitigation technology based on an improved understanding of the photosynthetic CO 2 fixation kinetics., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

To prevent interhuman transmission of viruses, new mask types─claiming improved filtration─require careful performance characterization. Here, a microfluidic spray device that can effectively simulate droplets emitted during coughing or sneezing was developed to spray droplets containing gold nanoparticles (AuNPs) that mimic SARS-CoV-2 to overcome the shortcomings associated with using biosamples. The light scattered by the AuNPs passing through the mask is successfully analyzed by using an automated scattering light mapping system within a duration of 2 min, thereby enabling high-throughput analysis of the filtering efficiency of various types of commercial masks. The differences in efficiency in terms of same mask type from different manufacturers, double masking, and prolonged usage, which are challenging to analyze with conventional testing systems, can also be assessed. AuNP-mediated mask performance evaluation enables the rapid determination of mask efficiency according to particle size and can contribute to the rapid response to counter new emerging infectious biohazards.

Objective: This study aimed to evaluate the association of periodontal disease with non-alcoholic fatty liver disease (NAFLD)., Materials and Methods: A retrospective follow-up study using the National Health Insurance Service-National Sample Cohort was performed from 2002 to 2015 in the Korean population. A total of 165,032 subjects were followed up for incident NAFLD during 11 years. Periodontal disease and NAFLD were defined by a diagnosis using the International Statistical Classification of Diseases and Related Health Problems, 10th revision (ICD-10) codes. Periodontal status was used as the severity of periodontal status and the number of dental visit due to PD., Results: Periodontitis was associated with a 4% increase in risk for NAFLD after adjusting for socio-demographic factor, health behaviors, and systemic diseases (adjusted hazard ratio [aHR] = 1.04, 95% CI = 1.01 to 1.07). Between the number of dental visit due to PD and the risk for NAFLD was observed a dose-effect association (aHR = 1.02, 95% CI = 0.99 to 1.05 for once; aHR = 1.10, 95% CI = 1.06 to 1.15 for two times; aHR = 1.14, 95% CI = 1.06 to 1.24 for three times)., Conclusions: Our data confirmed that periodontitis showed an association with a higher incidence of NAFLD., Clinical Relevance: Prevention and management of periodontal disease could be beneficial for reducing the risk of NAFLD., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

In this study, we present a method for synthesizing Ni-rich LiNi 0.93 Co 0.04 Al 0.03 O 2 (NCA) with a high-energy cathode material by the solid-phase method. The sintering temperature plays a very important role in the electrochemical performance of the LiNi 0.93 Co 0.04 Al 0.03 O 2 since it affects the crystallinity and structural stability. Therefore, various sintering temperatures (660 °C/690 °C/720 °C/750 °C/780 °C/810 °C) are studied to get optimum electrochemical performances. The electrochemical performance of LiNi 0.93 Co 0.04 Al 0.03 O 2 sintered at 720 °C shows the highest discharge capacity of 217.48 mAh g -1 with excellent Coulombic efficiency of 87.84% at 0.1 C. Moreover, the LiNi 0.93 Co 0.04 Al 0.03 O 2 sintered at 720 °C exhibits excellent rate-capability (181.1 mAh g -1 at 2.0 C) as well as superior cycle stability (95.4% after 80 cycles at 0.5 C). This is because optimized sintering temperature leads to good structural stability with low cation disorder and residual lithium content., (© 2022. The Author(s).)

The urinary exosomal miRNAs are recently emerging prostate cancer (PC)-associated biomarkers for the early-stage diagnosis and prognosis due to their non-invasiveness, inherent stability and the representation of the status of the originated cells. However, developing a urinary exosomal miRNA detection method with high accuracy is challenging because of the low abundance and high sequence homology of miRNAs. Herein, we present a quantitative and label-free miRNA sensing platform using surface-enhanced Raman scattering (SERS) based on three-dimensional (3D) hierarchical plasmonic nano-architecture to detect urinary exosomal miRNAs. This hierarchical nanostructure is constructed by self-assembly between target-complementary DNA probes-conjugated gold nanoparticles and head-flocked gold nanopillars in the presence of the target miRNAs, creating numerous 3D plasmonic hot-spots inducing exceedingly high amplification of SERS signals. This 3D SERS biosensor achieved ∼10 aM detection limits for the target miRNAs (miR-10a and miR-21), which is over 1000-fold more sensitive than previously reported miRNA sensors without the requirement of any labelling or pre-treatment steps. Finally, the clinical validation using urinary samples revealed that our 3D SERS sensor discriminates PC patients from healthy control with high diagnostic accuracy (0.93) based on the differential expression level of urinary exosomal miRNAs. These outputs demonstrate that our SERS sensor based on 3D hierarchical nano-architecture can offer facile, accurate and rapid methods to measure miRNA expression and is helpful for the diagnosis of various diseases., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Wastewater generated by the food industry is rich in nitrogen and phosphorus with possible presence of heavy metals. Physical and chemical methods of treatment, although effective, are expensive and may cause secondary environmental pollution damaging aquatic and human life. Traditional biological methods are eco-friendly and cost-effective but involve standalone microorganisms that pose risk of contamination and are not as effective. This review discusses the application of novel microalgal-bacterial consortium as a solution for the resource recovery and treatment of dairy, starch and aquaculture wastewater. Use of biofilm reactors containing anaerobic and aerobic sludge has shown 80-90% and > 90% COD and nutrient removal efficiency in treatment of dairy and starch processing wastewater, respectively. The treatment of aquaculture processing wastewater can be challenging due to high sality and requires salt-tolerant bacteria-microalgae consortium. In this regard, the identification of dominant microalgae and bacteria using 16S rRNA and 18S rRNA genes is recommended., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Microalgae are recognized as cell factories enriched with biochemicals suitable as feedstock for bio-energy, food, feed, pharmaceuticals, and nutraceuticals applications. The industrial application of microalgae is challenging due to hurdles associated with mass cultivation and biomass recovery. The scale-up production of microalgal biomass in freshwater is not a sustainable solution due to the projected increase of freshwater demands in the coming years. Microalgae cultivation in wastewater is encouraged in recent years for sustainable bioeconomy from biorefinery processes. Wastewater from the food industry is a less-toxic growth medium for microalgal biomass production. Traditional wastewater treatment and management processes are expensive; hence it is highly relevant to use low-cost wastewater treatment processes with revenue generation through different products. Microalgae are accepted as potential biocatalysts for the bioremediation of wastewater. Microalgae based purification of wastewater technology could be a universal alternative solution for the recovery of resources from wastewater for low-cost biomass feedstock for industry. This review highlights the importance of microalgal biomass production in food processing wastewater, their characteristics, and different microalgal cultivation methods, followed by nutrient absorption mechanisms. Towards the end of the review, different microalgae biomass harvesting processes with biorefinery products, and void gaps that tend to hinder the biomass production with future perspectives will be intended. Thus, the review could claim to be valuable for sustainable microalgae biomass production for eco-friendly bioproduct conversions., Competing Interests: Competing interestThe authors declare that they have no conflict of interest., (© The Author(s), under exclusive licence to Springer Nature B.V. 2022.)

There has been increasing attention in recent years on the use of photobioreactors for various biotechnological applications, especially for the cultivation of microalgae. Photobioreactors-based production of photosynthetic microorganisms furnish several advantages as minimising toxicity and providing improved conditions. However, the designing and scaling-up of photobioreactors (PBRs) remain a challenge. Due to huge capital investment and operating cost, there is a deficiency of suitable PBRs for development of photosynthetic microorganisms on large-scale. It is, therefore, highly desirable to understand the current state-of-the-art PBRs, their advantages and limitations so as to classify different PBRs as per their most suited applications. This review provides a holistic overview of the discreet features of diverse PBR designs and their purpose in microalgae growth and biohydrogen production and also summarizes the recent development in use of hybrid PBRs to increase their working efficiency and overall economics of their operation for the production of value-added products., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Alzheimer's disease (AD), the most common neurologic disorder, is characterized by progressive cognitive impairment. However, the low clinical significance of the currently used core AD biomarkers amyloid-beta and tau proteins remains a challenge. Recently, exosomes, found in human biological fluids, are gaining increasing attention because of their clinical significance in diagnosing of various diseases. In particular, blood-derived exosomal miRNAs are not only stable but also provide information regarding the different characteristics according to AD progression. However, quantitative and qualitative detection is difficult due to their characteristics, such as small size, low abundance, and high homology. Here, we present a DNA-assembled advanced plasmonic architecture (DAPA)-based plasmonic biosensor to accurately detect exosomal miRNAs in human serum. The designed nanoarchitecture possesses two narrow nanogaps that induce plasmon coupling; this significantly enhances its optical energy density, resulting in a 1.66-fold higher refractive-index (RI) sensitivity than nanorods at localized surface plasmon resonance (LSPR). Thus, the proposed biosensor is ultrasensitive and capable of selective single-nucleotide detection of exosomal miRNAs at the attomolar level. Furthermore, it identified AD patients from healthy controls by measuring the levels of exosomal miRNA-125b, miRNA-15a, and miRNA-361 in clinical serum samples. In particular, the combination of exosomal miRNA-125b and miRNA-361 showed the best diagnostic performance with a sensitivity of 91.67%, selectivity of 95.00%, and accuracy of 99.52%. These results demonstrate that our sensor can be clinically applied for AD diagnosis and has great potential to revolutionize the field of dementia research and treatment in the future., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Blood-based detection of Alzheimer's disease (AD) biomarker has become a prominent method for diagnosis of AD which can replace the complex and invasive cerebrospinal fluid (CSF)-based diagnostic method. However, the application of blood AD biomarker in actual AD diagnosis is hampered by the extremely low concentration of biomarkers in blood, as well as the existence of interfering proteins. Therefore, it is essential to develop a sensitive and specific detection platform to achieve blood-based diagnosis of AD. Here, a surface-enhanced Raman scattering (SERS)-based sensor is developed for the quantitative determination of tau protein in the plasma of AD patients. To acquire femtomolar-level detection limit, this platform involves the use of half antibody fragment immobilized onto head-flocked gold nanopillar SERS substrates and SERS-nanotags. The small size of the half antibody fragment maximizes the effect of plasmon coupling, by reducing the distance between SERS substrates and SERS-nanotags. Also, the use of half antibody fragment improves the antigen recognition ability by immobilizing the antibody with high density and efficient orientation of the antibody. The sensor using these characteristics showed a low detection limit of 3.21 fM and a wide detection range (10 fM - 1 μM). The platform was also able to accurately quantify the tau protein in the clinical plasma sample and correctly distinguish the AD patient from the healthy control. The ultrasensitive and specific SERS immunoassay platform facilitates accurate and early detection of AD biomarkers and can serve as a valuable tool for simple point-of-care testing in clinical diagnosis., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Background: The photosynthetic microorganism Chlamydomonas reinhardtii has been approved as generally recognized as safe (GRAS) recently, this can excessively produce carotenoid pigments and fatty acids. Zeaxanthin epoxidase (ZEP), which converts zeaxanthin to violaxanthin, and ADP-glucose pyrophosphorylase (AGP). These are key regulating genes for the xanthophyll and starch pathways in C. reinhardtii respectively. In this study, to produce macular pigment-enriched microalgal oil, we attempted to edit the AGP gene as an additional knock-out target in the zep mutant as a parental strain., Results: Using a sequential CRISPR-Cas9 RNP-mediated knock-out method, we generated double knock-out mutants (dZAs), in which both the ZEP and AGP genes were deleted. In dZA1, lutein (2.93 ± 0.22 mg g -1 DCW: dried cell weight), zeaxanthin (3.12 ± 0.30 mg g -1 DCW), and lipids (450.09 ± 25.48 mg g -1 DCW) were highly accumulated in N-deprivation condition. Optimization of the culture medium and process made it possible to produce pigments and oil via one-step cultivation. This optimization process enabled dZAs to achieve 81% higher oil productivity along with similar macular pigment productivity, than the conventional two-step process. The hexane/isopropanol extraction method was developed for the use of macular pigment-enriched microalgal oil for food. As a result, 196 ± 20.1 mg g -1 DCW of edible microalgal oil containing 8.42 ± 0.92 mg g -1 lutein of oil and 7.69 ± 1.03 mg g -1 zeaxanthin of oil was produced., Conclusion: Our research showed that lipids and pigments are simultaneously induced in the dZA strain. Since dZAs are generated by introducing pre-assembled sgRNA and Cas9-protein into cells, antibiotic resistance genes or selective markers are not inserted into the genome of dZA, which is advantageous for applying dZA mutant to food. Therefore, the enriched macular pigment oil extracted from improved strains (dZAs) can be further applied to various food products and nutraceuticals., (© 2022. The Author(s).)

Bisphenol A (or BPA) is a toxic endocrine disrupting chemical that is released into the environment through modern manufacturing practices. BPA can disrupt the production, function and activity of endogenous hormones causing irregularity in the hypothalamus-pituitary-gonadal glands and also the pituitary-adrenal function. BPA has immuno-suppression activity and can downregulate T cells and antioxidant genes. The genotoxicity and cytotoxicity of BPA is paramount and therefore, there is an immediate need to properly detect and remediate its influence. In this review, we discuss the toxic effects of BPA on different metabolic systems in the human body, followed by its mechanism of action. Various novel detection techniques (LC-MS, GC-MS, capillary electrophoresis, immunoassay and sensors) involving a pretreatment step (liquid-liquid microextraction and molecularly imprinted solid-phase extraction) have also been detailed. Mechanisms of various remediation strategies, including biodegradation using native enzymes, membrane separation processes, photocatalytic oxidation, use of nanosorbents and thermal degradation has been detailed. An overview of the global regulations pertaining to BPA has been presented. More investigations are required on the efficiency of integrated remediation technologies rather than standalone methods for BPA removal. The effect of processing operations on BPA in food matrices is also warranted to restrict its transport into food products., (Copyright © 2021 Elsevier B.V. All rights reserved.)

In the current global scenario, the world is under a serious dilemma due to the increasing human population, industrialization, and urbanization. The ever-increasing need for fuels and increasing nutritional problems have made a serious concern on the demand for nutrients and renewable and eco-friendly fuel sources. Currently, the use of fossil fuels is creating ecological and economic problems. Microalgae have been considered as a promising candidate for high-value metabolites and alternative renewable energy sources. Microalgae offer several advantages such as rapid growth rate, efficient land utilization, carbon dioxide sequestration, ability to cultivate in wastewater, and most importantly, they do not participate in the food crop versus energy crop dilemma or debate. An efficient microalgal biorefinery system for the production of lipids and subsequent byproduct for nutraceutical applications could well satisfy the need. But, the current microalgal cultivation systems for the production of lipids and nutraceuticals do not offer techno-economic feasibility together with energy and environmental sustainability. This review article has its main focus on the production of lipids and nutraceuticals from microalgae, covering the current strategies used for lipid production and the major high-value metabolites from microalgae and their nutraceutical importance. This review also provides insights on the future strategies for enhanced microalgal lipid production and subsequent utilization of microalgal biomass., Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest., (© The Author(s), under exclusive licence to Springer Nature B.V. 2021.)

Owing to its strong antioxidant properties, astaxanthin has a high market price in the nutraceutical and pharmaceutical fields, and its demand is increasing. Furthermore, with an increase in the demand for green technology, astaxanthin production through direct CO 2 conversion using the autotrophic green microalga Haematococcus pluvialis as a bio-platform has received much attention. Large-scale outdoor cultivation of H. pluvialis using waste CO 2 sources and sunlight can secure sustainability and improve economic efficiency. However, low strain performance, reduced light utilization because of increased cell density, and inefficient transfer of gaseous CO 2 into liquid culture broth hinder its large-scale commercialization of astaxanthin. Herein, we presented a multifaceted strategy, including the development of high-efficiency strains, a culture system for astaxanthin accumulation, and astaxanthin extraction from biomass, for economically producing astaxanthin from H. pluvialis through direct CO 2 conversion. Future perspectives were presented by comparing and analyzing various previous studies conducted using the latest technology., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Microalgae are unicellular photosynthetic organisms capable of producing high-value metabolites like carbohydrates, lipids, proteins, polyunsaturated fatty acids, vitamins, pigments, and other high-value metabolites. Microalgal biomass gained more interest for the production of nutraceuticals, pharmaceuticals, therapeutics, food supplements, feed, biofuel, bio-fertilizers, etc. due to its high lipid and other high-value metabolite content. Microalgal biomass has the potential to convert trapped solar energy to organic materials and potential metabolites of nutraceutical and industrial interest. They have higher efficiency to fix carbon dioxide (CO 2 ) and subsequently convert it into biomass and compounds of potential interest. However, to make microalgae a potential industrial candidate, cost-effective cultivation systems and harvesting methods for increasing biomass yield and reducing the cost of downstream processing have become extremely urgent and important. In this review, the current development in different microalgal cultivation systems and harvesting methods has been discussed., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Despite their excellent, useful, and stable properties, thermoplastics are constantly subject to environmental risks because of their low degradability under thermal, chemical, and mechanical stresses. To overcome the aforementioned issues, we hereby introduce an eco-friendly camphor (Ct) cyclic diester. The Ct diester is designed as a monomer, including a ketal group from the Ct, and shows high thermal stability via a rigid spiro-ring and a bridged bicyclic structure. A series of polyester was synthesized using the Ct diester, including various types of diols and dimethyl terephthalate. PETxCty copolyesters showed appropriate thermal stability up to 414 °C and a high glass transition temperature. This thermal behavior led to amorphous regions as the Ct diester content increased. Regarding the proportion of the Ct diester in the polyester, it was sensitive to hydrolysis and contributed to the degradation of the polyester in acid buffer conditions., Competing Interests: Conflict of interestThe authors declare no competing financial interest., (© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022.)

With industrialization, anthropogenic mishandlings have resulted in the discharge of abundant amount of CO 2 into the atmosphere. This has triggered an unnatural warming that has dramatically increased the Earth's temperature in a short duration. This problem can be addressed by the biological conversion of CO 2 ; several studies have been conducted using H. pluvialis culture that produces high value-added materials, such as astaxanthin and omega-3 fatty acids. However, although H. pluvialis has a high market value, the market size is quite small. Because H. pluvialis cells are susceptible to contamination due to its slow growth rate, hence large-scale culture of H. pluvialis without reliable contamination control strategies poses significant risks. This review comprehensively discusses the contamination that occurs during the culturing of H. pluvialis in various culture systems under different culture conditions. The review also discusses the strategies in controlling the biotic contaminants, such as bacteria and fungi., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Due to industrialization and rapid increase in world population, the global energy consumption has increased dramatically. As a consequence, there is increased consumption of fossil fuels, leading to a rapid increase in CO 2 concentration in the atmosphere. This accumulated CO 2 can be efficiently used by autotrophs as a carbon source to produce chemicals and biopolymers. There has been increasing attention on the production of polyhydroxybutyrate (PHB), a biopolymer, with focus on reducing the production cost. For this, cheaper renewable feedstocks, molecular tools, including metabolic and genetic engineering have been explored to improve microbial strains along with process engineering aspects for scale-up of PHB production. This review discusses the recent advents on the utilization of CO 2 as feedstock especially by engineered autotrophs, for sustainable production of PHB. The review also discusses the innovations in cultivation technology and process monitoring while understanding the underlying mechanisms for CO 2 to biopolymer conversion., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Modification of structural components, especially the cell wall, through adequate pretreatment strategies is critical to the bioconversion efficiency of algal biomass to biorefinery products. Over the years, several physical, physicochemical, chemical and green pretreatment methods have been developed to achieve maximum productivity of desirable by-products to sustain a circular bioeconomy. The effectiveness of the pretreatment methods is however, species specific due to diversity in the innate nature of the microalgal cell wall. This review provides a comprehensive overview of the most notable and promising pretreatment strategies for several microalgae species. Methods including the application of stress, ultrasound, electromagnetic fields, pressure, heat as well as chemical solvents (ionic liquids, supercritical fluids, deep eutectic solvents etc.) have been detailed and analyzed. Enzyme and hydrolytic microorganism based green pretreatment methods have also been reviewed. Metabolic engineering of microorganisms for product specificity and lower inhibitors can be a future breakthrough in microalgal pretreatment., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Haematococcus pluvialis has attracted interest as a bio-platform for producing omega-3 fatty acids (ω-3 FA) and astaxanthin that have a great potential as anti-inflammatory drugs. This study aimed to concurrently enhance the CO 2 fixation and the productivities of ω-3 FA and astaxanthin, which have been difficult to achieve because of the dissimilar culture methods for each goal, via calcium-mediated homeoviscous adaptation and biomineralization. As a result of 3 mM of Ca 2+ addition, ω-3 FA content was improved by 31% due to Ca 2+ -induced homeoviscous adaptation. Biomineralization was promoted by the extracellular carbonic anhydrase, which resulted in 46.3% improvement in CO 2 fixation. CaCO 3 from the biomineralization was beneficially re-used in the H. pluvialis culture and triggered 178- and 522-fold increased biomass productivity and astaxanthin content, respectively, thanks to its anisotropic nature. The Ca 2+ -based productivity enhancement strategy was applied to large-scale culture which resulted improvement in overall bioprocess performance., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Microalgae can accumulate various carbon-neutral products, but their real-world applications are hindered by their CO 2 susceptibility. Herein, the transcriptomic changes in a model microalga, Chlamydomonas reinhardtii, in a high-CO 2 milieu (20%) are evaluated. The primary toxicity mechanism consists of aberrantly low expression of plasma membrane H + -ATPases (PMAs) accompanied by intracellular acidification. Our results demonstrate that the expression of a universally expressible PMA in wild-type strains makes them capable of not only thriving in acidity levels that they usually cannot survive but also exhibiting 3.2-fold increased photoautotrophic production against high CO 2 via maintenance of a higher cytoplasmic pH. A proof-of-concept experiment involving cultivation with toxic flue gas (13 vol% CO 2 , 20 ppm NO X , and 32 ppm SO X ) shows that the production of CO 2 -based bioproducts by the strain is doubled compared with that by the wild-type, implying that this strategy potentially enables the microalgal valorization of CO 2 in industrial exhaust., (© 2021. The Author(s).)

The microalgae Haematococcus pluvialis biologically converts CO 2 into natural astaxanthin that possesses a strong antioxidant activity; its low carbon footprint and economic viability have allowed it to garner great attention. However, low efficiency of the cultivation process is a major hurdle in its commercial production. Here, additional nitrogen was provided to fully grown cells to induce efficient cyst germination, thereby rapidly increasing the number of cells containing astaxanthin. After germination, these cells were rapidly converted from zooids to cysts by nitrogen depletion, while maintaining a steady astaxanthin content of 5.5%. Consequently, a 2.1-fold increase in astaxanthin productivity in comparison with that in a batch culture was achieved. Moreover, the germination-based semi-continuous process yielded 2.6 times higher astaxanthin productivity in a large-scale culture using power plant flue gas and a polymeric photobioreactor. This study provides a promising cultivation strategy for the commercial mass production of natural astaxanthin., (Copyright © 2021 Elsevier Ltd. All rights reserved.)