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5. ICT Services for Electro-Mobility

Author

Rinaldi, A., Amditis, A., Berardi, A., Fanti, M.P., Giansante, C., Launo, C., Mangini, A.M., Porru, S., Portouli, E., Roccotelli, M., Silvestri, B., Ukovich, W., and Winder, A.

Subjects
ICT services, smart mobility, electromobility, light electric vehicles, urban mobility, urban transport
Abstract

The evolution of mobility in a sustainable perspective requires specific attention to the end-users roles and the changing needs of the cities and policies. This work shows how ICT (Information and Communications Technology) services can allow the transition towards smart and sustainable mobility. Within the European project H2020 ELVITEN (GA nr. 769926), a proper set of ICT services are described, including the brokering and the booking service for vehicles and charging stations, the ICT platform for monitoring the fleet of electric vehicles, the serious game app and a smart app for incentives. These services are suitable and indispensable to incentivize and encourage users towards a new mobility that is electric and sustainable.

Published
2020

17. Prognostic Impact of Diabetes and Prediabetes on Survival Outcomes in Patients With Chronic Heart Failure: A Post‐Hoc Analysis of the GISSI‐HF (Gruppo Italiano per lo Studio della Sopravvivenza nella Insufficienza Cardiaca‐Heart Failure) Trial

Author

Dauriz, Marco, primary, Targher, Giovanni, additional, Temporelli, Pier Luigi, additional, Lucci, Donata, additional, Gonzini, Lucio, additional, Nicolosi, Gian Luigi, additional, Marchioli, Roberto, additional, Tognoni, Gianni, additional, Latini, Roberto, additional, Cosmi, Franco, additional, Tavazzi, Luigi, additional, Maggioni, Aldo Pietro, additional, Barlera, Simona, additional, Franzosi, Maria Grazia, additional, Maggioni, Aldo P., additional, Porcu, Maurizio, additional, Yusuf, Salim, additional, Camerini, Fulvio, additional, Cohn, Jay N., additional, Decarli, Adriano, additional, Pitt, Bertram, additional, Sleight, Peter, additional, Poole‐Wilson, Philip A., additional, Geraci, Enrico, additional, Scherillo, Marino, additional, Fabbri, Gianna, additional, Bartolomei, Barbara, additional, Bertoli, Daniele, additional, Cobelli, Franco, additional, Fresco, Claudio, additional, Ledda, Antonietta, additional, Levantesi, Giacomo, additional, Opasich, Cristina, additional, Rusconi, Franco, additional, Sinagra, Gianfranco, additional, Turazza, Fabio, additional, Volpi, Alberto, additional, Ceseri, Martina, additional, Alongi, Gianluca, additional, Atzori, Antonio, additional, Bambi, Filippo, additional, Bastarolo, Desiree, additional, Bianchini, Francesca, additional, Cangioli, Iacopo, additional, Canu, Vittoriana, additional, Caporusso, Concetta, additional, Cenni, Gabriele, additional, Cintelli, Laura, additional, Cocchio, Michele, additional, Confente, Alessia, additional, Fenicia, Eva, additional, Friso, Giorgio, additional, Gianfriddo, Marco, additional, Grilli, Gianluca, additional, Lazzaro, Beatrice, additional, Lonardo, Giuseppe, additional, Luise, Alessia, additional, Nota, Rachele, additional, Orlando, Mariaelena, additional, Petrolo, Rosaria, additional, Pierattini, Chiara, additional, Pierota, Valeria, additional, Provenzani, Alessandro, additional, Quartuccio, Velia, additional, Ragno, Anna, additional, Serio, Chiara, additional, Spolaor, Alvise, additional, Tafi, Arianna, additional, Tellaroli, Elisa, additional, Ghio, Stefano, additional, Ghizzardi, Elisa, additional, Masson, Serge, additional, Crociati, Lella, additional, La Rovere, Maria Teresa, additional, Corrà, Ugo, additional, Finzi, Andrea, additional, Gorini, Marco, additional, Milani, Valentina, additional, Orsini, Giampietro, additional, Bianchini, Elisa, additional, Cabiddu, Silvia, additional, Cangioli, Ilaria, additional, Cipressa, Laura, additional, Cipressa, Maria Lucia, additional, Di Bitetto, Giuseppina, additional, Ferri, Barbara, additional, Galbiati, Luisa, additional, Lorimer, Andrea, additional, Pera, Carla, additional, Priami, Paola, additional, Vasamì, Antonella, additional, Moccetti, T., additional, Rossi, M.G., additional, Pasotti, E., additional, Vaghi, F., additional, Roncarolo, P., additional, Zunino, M.T., additional, Matta, F., additional, Perinetto, E. Actis, additional, Gaita, F., additional, Azzaro, G., additional, Zanetta, M., additional, Paino, A.M., additional, Parravicini, U., additional, Vegis, D., additional, Conte, R., additional, Ferraro, P., additional, De Bernardi, A., additional, Morelloni, S., additional, Fagnani, M., additional, Lucchina, P. Greco, additional, Montagna, L., additional, Bellone, E., additional, Sappè, D., additional, Ferraro, F., additional, Delucchi, M., additional, Reynaud, S.G., additional, Dore, M., additional, La Brocca, A., additional, Massobrio, N., additional, Bo, L., additional, Trinchero, R., additional, Imazio, M., additional, Brocchi, G., additional, Nejrotti, A., additional, Rissone, L., additional, Gabasio, S., additional, Zocchi, C., additional, Randazzo, S., additional, Crenna, A., additional, Giannuzzi, P., additional, Bonanomi, E., additional, Mezzani, A., additional, De Marchi, M., additional, Begliuomini, G., additional, Gianonatti, C.A., additional, Gavazzi, A., additional, Grosu, A., additional, Dei Cas, L., additional, Nodari, S., additional, Garyfallidis, P., additional, Bertoletti, A., additional, Bonifazi, C., additional, Arisi, S., additional, Mascaro, F., additional, Fraccarollo, M., additional, Dell'Orto, S., additional, Sfolcini, M., additional, Bortolini, F., additional, Raccagni, D., additional, Turelli, A., additional, Santarone, M., additional, Miglierina, E., additional, Sormani, L., additional, Jemoli, R., additional, Tettamanti, F., additional, Pirelli, S., additional, Bianchi, C., additional, Verde, S., additional, Mariani, M., additional, Ziacchi, V., additional, Ferrazza, A., additional, Russo, A., additional, Bortolotti, M., additional, Pasini, G.F., additional, Volpi, A., additional, Jones, K.N., additional, Cuzzucrea, D., additional, Gullace, G., additional, Carbone, C., additional, Granata, A., additional, De Servi, S., additional, Del Rosso, G., additional, Inserra, C., additional, Renaldini, E., additional, Zappa, C., additional, Moretti, M., additional, Zanini, R., additional, Ferrari, M., additional, Moroni, E., additional, Cei, A., additional, Lissi, C., additional, Dovico, E., additional, Fiorentini, C., additional, Palermo, P., additional, Brusoni, B., additional, Negrini, M., additional, Heyman, J., additional, Danzi, G.B., additional, Finzi, A., additional, Frigerio, M., additional, Turazza, F., additional, Beretta, L., additional, Sachero, A., additional, Casazza, F., additional, Squadroni, L., additional, Lombardi, F., additional, Marano, L., additional, Margonato, A., additional, Fragasso, G., additional, Febo, O.C., additional, Aiolfi, E., additional, Olmetti, F., additional, Grieco, A., additional, Antonazzo, V., additional, Specchia, G., additional, Mortara, A., additional, Robustelli, F., additional, Songini, M.G., additional, Schweiger, C., additional, Frisinghelli, A., additional, Palvarini, M., additional, Campana, C., additional, Scelsi, L., additional, Ajmone Marsan, N., additional, Cobelli, F., additional, Gualco, A., additional, Opasich, C., additional, De Feo, S., additional, Mazzucco, R., additional, Iannone, M.A., additional, Diaco, T., additional, Zaniboni, D., additional, Milanesi, G., additional, Nassiacos, D., additional, Meloni, S., additional, Giani, P., additional, Nicoli, T., additional, Malinverni, C., additional, Gusmini, A., additional, Pozzoni, L., additional, Bisiani, G., additional, Margaroli, P., additional, Schizzarotto, A., additional, Daverio, A., additional, Occhi, G., additional, Partesana, N., additional, Bandini, P., additional, Rosella, M.G., additional, Giustiniani, S., additional, Cucchi, G., additional, Pedretti, R., additional, Raimondo, R., additional, Vaninetti, R., additional, Fedele, A., additional, Ghezzi, I., additional, Rezzonico, E., additional, Salerno Uriarte, J.A., additional, Morandi, F., additional, Salvucci, F., additional, Valenti, C., additional, Graziano, G., additional, Romanò, M., additional, Cimminiello, C., additional, Mangone, I., additional, Lombardo, M., additional, Quorso, P., additional, Marinoni, G., additional, Breghi, M., additional, Erckert, M., additional, Dienstl, A., additional, Mirante Marini, G., additional, Stefenelli, C., additional, Cioffi, G., additional, Buczkowska, E., additional, Bonanome, A., additional, Bazzanini, F., additional, Parissenti, L., additional, Serafini, C., additional, Catania, G., additional, Tarantini, L., additional, Rigatelli, G., additional, Boni, S., additional, Pasini, A., additional, Masini, E., additional, Zampiero, A.A., additional, Zanchetta, M., additional, Franceschetto, L., additional, Delise, P., additional, Marcon, C., additional, Sacchetta, A., additional, Borgese, L., additional, Artusi, L., additional, Casolino, P., additional, Corbara, F., additional, Banzato, A., additional, Barbiero, M., additional, Aldegheri, M.P., additional, Bazzucco, R., additional, Crivellenti, G., additional, Raviele, A., additional, Zanella, C., additional, Pascotto, P., additional, Sarto, P., additional, Milan, S., additional, Barbieri, E., additional, Girardi, P., additional, Dalla Villa, W., additional, Dalle Mule, J., additional, Di Sipio, M.L., additional, Cazzin, R., additional, Milan, D., additional, Zonzin, P., additional, Carraro, M., additional, Rossi, R., additional, Carbonieri, E., additional, Rossi, I., additional, Stritoni, P., additional, Meneghetti, P., additional, Risica, G., additional, Tenderini, P.L., additional, Vassanelli, C., additional, Zanolla, L., additional, Perini, G., additional, Brighetti, G., additional, Chiozza, R., additional, Giuliano, G., additional, Gortan, R., additional, Cesanelli, R., additional, Nicolosi, G.L., additional, Piazza, R., additional, Mos, L., additional, Vriz, O., additional, Pavan, D., additional, Pascottini, G., additional, Alberti, E., additional, Werren, M., additional, Solinas, L., additional, Sinagra, G., additional, Longaro, F., additional, Fioretti, P., additional, Albanese, M.C., additional, Miani, D., additional, Gianrossi, R., additional, Pende, A., additional, Rubartelli, P., additional, Magaia, O., additional, Domenicucci, S., additional, Caruso, D., additional, Faraguti, A.S., additional, Magliani, L., additional, Miccoli, F., additional, Guglielmino, G., additional, Bertoli, D., additional, Cantarelli, A., additional, Orlandi, S., additional, Vallebona, A., additional, Pozzati, A., additional, Brega, G., additional, Pancaldi, L.G., additional, Vandelli, R., additional, Urbinati, S., additional, Poci, M.G., additional, Zoli, M., additional, Costa, G.M., additional, Guiducci, U., additional, Zobbi, G., additional, Tartagni, F., additional, Tisselli, A., additional, Gentili, A., additional, Pieri, P., additional, Cagnetta, E., additional, Bendinelli, S., additional, Barbieri, A., additional, Conti, R., additional, Ferrari, R., additional, Merlini, F., additional, Fucili, A., additional, Moruzzi, P., additional, Buia, E., additional, Galvani, M., additional, Ferrini, D., additional, Baggioni, G., additional, Yiannacopulu, P., additional, Canè, G., additional, Bonfiglioli, A., additional, Zandomeneghi, R., additional, Brugioni, L., additional, Giannini, A., additional, Di Ruvo, R., additional, Giuliani, M., additional, Rusconi, L., additional, Del Corso, P., additional, Piovaccari, G., additional, Bologna, F., additional, Venturi, P., additional, Melandri, F., additional, Bagni, E., additional, Bolognese, L., additional, Perticucci, R., additional, Zuppiroli, A., additional, Nannini, M., additional, Consoli, N., additional, Petrone, P., additional, Pipitò, C., additional, Colombi, L., additional, Bernardi, D., additional, Mariani, P.R., additional, Testa, R., additional, Mazzinghi, F., additional, Cosmi, F., additional, Cosmi, D., additional, Zipoli, A., additional, Cecchi, A., additional, Castelli, G., additional, Ciaccheri, M., additional, Mori, F., additional, Pieri, F., additional, Valoti, P., additional, Chiarantini, D., additional, Santoro, G.M., additional, Minneci, C., additional, Marchi, F., additional, Milli, M., additional, Zambaldi, G., additional, Brandinelli Geri, A.A., additional, Cipriani, M., additional, Alessandri, M., additional, Severi, S., additional, Stefanelli, S., additional, Comella, A., additional, Poddighe, R., additional, Digiorgio, A., additional, Carluccio, M., additional, Berti, S., additional, Rizza, A., additional, Bonatti, V., additional, Molendi, V., additional, Brancato, A., additional, D'Aprile, N., additional, Giappichini, G., additional, Del Vecchio, S., additional, Mantini, G., additional, De Tommasi, F., additional, Meucci, G., additional, Cordoni, M., additional, Bechi, S., additional, Barsotti, L., additional, Baldini, P., additional, Romei, M., additional, Scopelliti, G., additional, Lauri, G., additional, Pestelli, F., additional, Furiozzi, F., additional, Cocchieri, M., additional, Severini, D., additional, Patriarchi, F., additional, Chiocchi, P., additional, Buccolieri, M., additional, Martinelli, S., additional, Wee, A., additional, Angelici, F., additional, Bernardinangeli, M., additional, Proietti, G., additional, Biscottini, B., additional, Panciarola, R., additional, Marinacci, L., additional, Perna, G.P., additional, Gabrielli, D., additional, Moraca, A., additional, Moretti, L., additional, Partemi, L., additional, Gregori, G., additional, Amici, R., additional, Patteri, G., additional, Capone, P., additional, Savini, E., additional, Morgagni, G.L., additional, Paccaloni, L., additional, Pezzuoli, F., additional, Carincola, S., additional, Papi, S., additional, De Crescentini, S., additional, Gerardi, P., additional, Midi, P., additional, Gallenzi, E., additional, Pajes, G., additional, Mancone, C., additional, Di Spirito, V., additional, Di Gennaro, M., additional, Calcagno, S., additional, Toscano, S., additional, Antonicoli, S., additional, Carta, F., additional, Giorgi, G., additional, Comito, F., additional, Daniele, E., additional, Ciarla, O., additional, Gelfo, P.G., additional, Acquaviva, A., additional, Testa, D., additional, Testa, G., additional, Pagliaro, F.A., additional, Russo, F., additional, Vetta, F., additional, Marchese, I., additional, Di Sciascio, G., additional, D'Ambrosio, A., additional, Leggio, F., additional, Del Sindaco, D., additional, Lacchè, A., additional, Avallone, A., additional, Risa, M.P., additional, Azzolini, P., additional, Baldo, E., additional, Giovannini, E., additional, Pulignano, G., additional, Tondo, C., additional, Picchio, E., additional, ani, E., additional, Tanzi, P., additional, Pozzar, F., additional, Farnetti, F., additional, Azzarito, M., additional, Santini, M., additional, Varveri, A., additional, Ferraiuolo, G., additional, Valtorta, C., additional, Gaspardone, A., additional, Barbato, G., additional, Ceci, V., additional, Aspromonte, N., additional, Bellocci, F., additional, Colizzi, C., additional, Fedele, F., additional, Perez, F.I., additional, Galati, A., additional, Rossetti, A., additional, Mainella, A., additional, etta, D., additional, Matteucci, C., additional, Busi, G., additional, De Angelis, A., additional, Farina, G., additional, Granatelli, A., additional, Leone, F., additional, Frasca, F., additional, Di Giovambattista, R., additional, Castellani, G., additional, Massaro, G., additional, Mastrogiuseppe, G., additional, Vacri, A., additional, De Sanctis, F., additional, Cioli, M., additional, Di Luzio, S., additional, Napoletano, C., additional, Piccioni, L.L., additional, De Simone, G., additional, Ottaviano, A., additional, Mazza, V., additional, Spedaliere, C., additional, Staniscia, D., additional, Calgione, E., additional, De Marco, G., additional, Chiacchio, T., additional, Di Napoli, T., additional, Romanzi, S., additional, Salvatore, G., additional, Golino, P., additional, Palermo, A., additional, Mascia, F., additional, Vetrano, A., additional, Vinciguerra, A., additional, Caliendo, L., additional, Longobardi, R., additional, De Caro, G., additional, Di Nola, R., additional, Piemonte, F., additional, Prinzi, D., additional, De Rosa, P., additional, De Rosa, V., additional, Riello, F., additional, Capuano, V., additional, Vecchio, G., additional, Landi, M., additional, Amato, S., additional, Garofalo, M., additional, D'Avino, M., additional, Sensale, P., additional, Maiolica, O., additional, Santoro, R., additional, Caso, P., additional, Miceli, D., additional, Maurea, N., additional, Bianchi, U., additional, Crispo, C., additional, Chiariello, M., additional, Perrone Filardi, P., additional, Russo, L., additional, Capuano, N., additional, Ungaro, G., additional, Vergara, G., additional, Scafuro, F., additional, D'Angelo, G., additional, Campaniello, C., additional, Bottiglieri, P., additional, Volpe, A., additional, Battista, R., additional, De Risi, L., additional, Cardillo, G., additional, Sibilio, G., additional, Marino, A.P., additional, Silvestri, F., additional, Predotti, P., additional, Iervoglini, A., additional, De Matteis, C., additional, Sarnicola, P., additional, Matarazzo, M.M., additional, Baldi, S., additional, Iuliano, V., additional, Astarita, C., additional, Cuccaro, P., additional, Liguori, A., additional, Liguori, G., additional, Gregorio, G., additional, Petraglia, L., additional, Antonelli, G., additional, Amodio, G., additional, De Luca, I., additional, Traversa, D., additional, Franchini, G., additional, Lenti, M.L., additional, Cavallari, D., additional, D'Agostino, C., additional, Scalera, G., additional, Altamura, C.M., additional, Russo, M., additional, Mascolo, A.R., additional, Pettinati, G., additional, Ciricugno, S.A., additional, Scrutinio, D., additional, Passantino, A., additional, Mastrangelo, D., additional, Di Masi, A., additional, De Carne, R., additional, Cannone, M., additional, Dibiase, F., additional, Pensato, M., additional, Loliva, F., additional, Trapani, F., additional, Panettieri, I., additional, Leone, L., additional, Di Biase, M., additional, Carrone, M., additional, Gallone, V., additional, Cocco, F., additional, Costantini, M., additional, Tritto, C., additional, Cavalieri, F., additional, Stella, L., additional, Magliari, F., additional, Callerame, M., additional, De Giorgi, A., additional, Pellegrino, L., additional, Correra, M., additional, Portulano, V., additional, Nisi, G.L., additional, Grassi, G., additional, Cristallo, E., additional, De Laura, D., additional, Salerno, C., additional, Fanelli, R., additional, Villella, M., additional, Pede, S., additional, Renna, A., additional, De Lorenzi, E., additional, Urso, L., additional, Lenti, V., additional, Peluso, A., additional, Baldi, N., additional, Polimeni, G., additional, Palma, P., additional, Lauletta, R., additional, Tagliamonte, E., additional, Cirillo, T., additional, Silvestri, B., additional, Centonze, G., additional, D'Alessandro, B., additional, Truncellito, L., additional, Mecca, D., additional, Petruzzi, M.A., additional, Coviello, R.O.M., additional, Lopizzo, A., additional, telli, M., additional, Barbuzzi, S., additional, Gubelli, S., additional, Germinario, G., additional, Cosentino, N., additional, Mingrone, A., additional, Vico, R., additional, Borrello, G., additional, Mazza, M.L., additional, Cimino, R., additional, Galasso, D., additional, Cassadonte, F., additional, Talarico, U., additional, Perticone, F., additional, Cassano, S., additional, Catapano, F., additional, Calemme, S., additional, Feraco, E., additional, Cloro, C., additional, Misuraca, G., additional, Caporale, R., additional, Vigna, L., additional, Spagnuolo, V., additional, De Rosa, F., additional, Spadafora, G., additional, Zampaglione, G., additional, Russo, R., additional, Schipani, F.A., additional, Ferragina, A.F., additional, Stranieri, D., additional, Musca, G., additional, Carpino, C., additional, Bencardino, P., additional, Raimondo, F., additional, Musacchio, D., additional, Pulitanò, G., additional, Ruggeri, A., additional, Provenzano, A., additional, Salituri, S., additional, Musolino, M., additional, Calandruccio, S., additional, Marrari, A., additional, Tripodi, E., additional, Scali, R., additional, Anastasio, L., additional, Arone, A., additional, Aragona, P., additional, Donnangelo, L., additional, Comito, M.G.A., additional, Bilotta, F., additional, Vaccaro, I., additional, Rametta, R., additional, Ventura, V., additional, Bonvegna, A., additional, Alì, A., additional, Cinnirella, C., additional, Raineri, M., additional, Pompeo, F., additional, Cascio Ingurgio, N., additional, Carini, V., additional, Coco, R., additional, Giunta, G., additional, Leonardi, G., additional, Randazzo, V., additional, Di Blasi, V., additional, Tamburino, C., additional, Russo, G., additional, Mangiameli, S., additional, Cardillo, R., additional, Castelli, D., additional, Inserra, V., additional, Arena, A., additional, Gulizia, M.M., additional, Raciti, S., additional, Rapisarda, G., additional, Romano, R., additional, Prestifilippo, P., additional, Braschi, G.B., additional, Ledda, G., additional, Terrazzino, R., additional, De Caro, M., additional, Scilabra, G., additional, agnino, B., additional, Grassi, R., additional, Di Tano, G., additional, Scimone, G.F., additional, Vasquez, L., additional, Coppolino, C., additional, Casale, A., additional, Castelli, M., additional, D'Urso, G., additional, D'Antonio, E., additional, Lo Presti, L., additional, Badalamenti, E., additional, Conti, P., additional, Sanfilippo, N., additional, Cirrincione, V., additional, Cinà, M.T., additional, Cusimano, G., additional, Taormina, A., additional, Giuliano, P., additional, Bajardi, A., additional, Mandalà, V., additional, Canonico, A., additional, Geraci, G., additional, Sabella, F.P., additional, Enia, F., additional, Floresta, A.M., additional, Lo Cascio, I., additional, Gumina, D., additional, Cavallaro, A., additional, Piccione, G., additional, Ferrante, R., additional, Blandino, M., additional, Iudicello, M.S., additional, Mossuti, E., additional, Romano, G., additional, Lombardo, L., additional, Monastra, P., additional, Di Vincenzo, D., additional, Porcu, M., additional, Orrù, P., additional, Muscas, F., additional, Giardina, G., additional, Corda, M., additional, Locci, G., additional, Podda, A., additional, Ledda, M., additional, Siddi, P., additional, Lai, C., additional, Pili, G., additional, Mercuro, G., additional, Mureddu, G., additional, Ganau, A., additional, Meloni, G., additional, Poddighe, G., additional, and Sanna, G., additional

Published
2017
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18. Defects in the Amorphous–Crystalline Evolution of Gel-Derived TiO2

Author

Koral, C., Fantauzzi, M., Imparato, C., Papari, G. P., Silvestri, B., Aronne, A., Andreone, A., and Rossi, A.

Abstract

The catalytic and electro-optical properties of TiO2-based materials depend on the defectivity of their electronic structures and in turn on the synthesis conditions. It is however less clear how to control the number of defects in this oxide. In this paper, spectroscopic and structural characterization of chemical [acetylacetonate–TiO2, hybrid gel, hybrid sol–gel titania (HSGT)] and particulate [inorganic gel, sol–gel titania (SGT)] TiO2amorphous gels is presented. HSGT and SGT were thermally treated at different annealing temperatures and at various times, obtaining anatase or rutile samples. X-ray diffraction, X-ray photoelectron spectroscopy (XPS), and terahertz-time domain spectroscopy (THz-TDS) were exploited for monitoring changes in the crystallographic structure, chemical composition, and dielectric function. It is found that the thermal treatment affects in different ways the hybrid and the particulate TiO2gels, resulting in an increase of electron transfer in the former. Moreover, XPS combined with X-ray-induced Auger electron spectroscopy provides evidence that the ionicity of the bond is also different and well correlates with the number of defects. These results demonstrate that XPS and THz-TDS techniques can be used as complementary tools for determining the electronic properties of titania with various degrees of crystallinity and defects.

Published
2020
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19. Collaborative research and action to control the geographic placement of outdoor advertising of alcohol and tobacco products in Chicago

Author

Hackbarth, D. P., Schnopp-Wyatt, D., Katz, D., Williams, J., Silvestri, B., and Pfleger, M.

Subjects
Lung Neoplasms, Adolescent, Universities, Smoking Prevention, Tobacco Industry, Community Health Planning, Advertising, Residence Characteristics, Humans, Voluntary Health Agencies, Cooperative Behavior, Child, Minority Groups, Chicago, Electronic Data Processing, Geography, Smoking, Catholicism, Community Participation, Urban Health, Public Health, Environmental and Occupational Health, Hispanic or Latino, Black or African American, Cross-Sectional Studies, Socioeconomic Factors, Social Conditions, Small-Area Analysis, Health Services Research, Research Article
Abstract

Community activists in Chicago believed their neighborhoods were being targeted by alcohol and tobacco outdoor advertisers, despite the Outdoor Advertising Association of America's voluntary code of principles, which claims to restrict the placement of ads for age-restricted products and prevent billboard saturation of urban neighborhoods. A research and action plan resulted from a 10-year collaborative partnership among Loyola University Chicago, the American Lung Association of Metropolitan Chicago (ALAMC), and community activists from a predominately African American church, St. Sabina Parish. In 1997 Loyola University and ALAMC researchers conducted a cross-sectional prevalence survey of alcohol and tobacco outdoor advertising. Computer mapping was used to locate all 4,247 licensed billboards in Chicago that were within 500- and 1,000-foot radiuses of schools, parks, and playlots. A 50% sample of billboards was visually surveyed and coded for advertising content. The percentage of alcohol and tobacco billboards within the 500- and 1,000-foot zones ranged from 0% to 54%. African American and Hispanic neighborhoods were disproportionately targeted for outdoor advertising of alcohol and tobacco. Data were used to convince the Chicago City Council to pass one of the nation's toughest anti-alcohol and tobacco billboard ordinances, based on zoning rather than advertising content. The ordinance was challenged in court by advertisers. Recent Supreme Court rulings made enactment of local billboard ordinances problematic. Nevertheless, the research, which resulted in specific legislative action, demonstrated the importance of linkages among academic, practice, and grassroots community groups in working together to diminish one of the social causes of health disparities.

Published
2001

20. Lymphoproliferative Disease in Human Peripheral Blood Mononuclear Cell-Injected SCID Mice. IV. Differential Activation of Human Th1 and Th2 Lymphocytes and Influence of the Atopic Status on Lymphoma Development

Author

Vincenzo Coppola, Veronesi, A., Indraccolo, S., Calderazzo, F., Mion, M., Minuzzo, S., Esposito, G., Mauro, D., Silvestri, B., Gallo, P., Falagiani, P., Amadori, A., and Chieco-Bianchi, L.

Subjects
Adult, Hypersensitivity, Immediate, Male, Mice, Inbred BALB C, Lymphoma, Immunology, Mice, SCID, Immunoglobulin E, Th1 Cells, Flow Cytometry, Lymphocyte Activation, Interferon-gamma, Mice, Th2 Cells, Antigens, Heterophile, Immunoglobulin G, Leukocytes, Mononuclear, Animals, Humans, Immunology and Allergy, Female, Interleukin-4, RNA, Messenger, Injections, Intraperitoneal
Abstract

Intraperitoneal transfer of PBMC from EBV+ donors into SCID mice leads to high human Ig levels in mouse serum and B cell lymphoproliferative disease. As these events depend on the activation of coinjected human T cells, we addressed the behavior of the Th1 and Th2 subsets in this model. Production of IFN-γ, but not of Th2 cytokines such as IL-4, was detected in culture supernatants of PBMC stimulated in vitro with mouse splenocytes. Moreover, anti-CD3 stimulation of the human cells recovered from mice brought about IFN-γ, but not IL-4, synthesis; on the other hand, PCR and in situ hybridization analysis of ex vivo-recovered cells disclosed the presence of mRNA for both cytokines following in vitro restimulation, thus suggesting post-transcriptional regulation of IL-4 gene expression. When SCID mice were inoculated with PBMC from atopic donors, whose Th1/Th2 profile displays an imbalance toward Th2 cells, tumor development rates were lower, and tumor latency was higher, compared with those in mice injected with PBMC from normal donors. Isotypic analysis of human Ig in mouse serum showed the exclusive presence of IFN-γ-driven IgG subclasses; in addition, human IgE were low or undetectable in most cases. These findings indicate that following transfer into SCID mice, human Th1 lymphocytes undergo preferential activation, whereas Th2 function is down-regulated. Th1 lymphocytes probably are a major component in promoting EBV+ B cell expansion and tumor development; the individual Th1/Th2 profile could in part account for the as yet unexplained donor variability in tumor generation in this experimental model.

Published
1998

21. Thermo-mechanical behavior of poly(butylene terephthalate)/silica nanocomposites.

Author

Russo, P., Costantini, A., Luciani, G., Tescione, F., Lavorgna, M., Branda, F., and Silvestri, B.

Subjects
POLYBUTYLENE terephthalate, NANOCOMPOSITE materials, SILICA nanoparticles, THERMOGRAVIMETRY, GLASS transition temperature
Abstract

ABSTRACT In this work, experimental results about poly(butylene terephthalate) (PBT) based nanocomposites filled with various amounts of silica nanoparticles (NPs) are reported. Two different types of filler are used: silica gel NPs, produced through the Stöber method, and a commercial fumed silica, both coated by a PBT shell. Melt-mixed samples have been thoroughly investigated by scanning and transmission electron microscopy, infrared Fourier transform spectroscopy (FTIR), thermal gravimetric analysis, differential scanning calorimetry, wide and small angle X-ray diffraction, and dynamic mechanical analysis. A fine and very good dispersion of NPs into the polymeric matrix is revealed through the morphological analysis when Stöber NPs were used as filler with respect to systems including commercial fumed silica particles. This evidence, combined with matrix-filler interactions revealed by FTIR spectroscopy, justifies the enhancement of both storage modulus and glass transition temperature of the former samples in comparison with reference pristine PBT. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 46006. [ABSTRACT FROM AUTHOR]

Published
2018
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30. Differential effect on TCR:CD3 stimulation of a 90-kD glycoprotein (gp90/Mac-2BP), a member of the scavenger receptor cysteine-rich domain protein family.

Author

Silvestri, B, Calderazzo, F, Coppola, V, Rosato, A, Iacobelli, S, Natoli, C, Ullrich, A, Sures, I, Azam, M, Brakebusch, Cord Herbert, Chieco-Bianchi, L, Amadori, A, Silvestri, B, Calderazzo, F, Coppola, V, Rosato, A, Iacobelli, S, Natoli, C, Ullrich, A, Sures, I, Azam, M, Brakebusch, Cord Herbert, Chieco-Bianchi, L, and Amadori, A

Abstract

Udgivelsesdato: 1998-Sep, We studied the effects of a 90-kD glycoprotein (gp90/Mac-2BP) belonging to the scavenger receptor family, present in normal serum and at increased levels in inflammatory disease and cancer patients, on some T cell function parameters. Whereas the lymphocyte proliferative response to non-specific mitogens such as phytohaemagglutinin (PHA) and concanavalin A (Con A), but not pokeweed mitogen (PWM), was strongly reduced, probably due to the lectin-binding properties of gp90/Mac-2BP, the response to T cell receptor (TCR) agonists such as superantigens and allogeneic cells was potentiated. When lymphocytes were stimulated with different anti-TCR:CD3 MoAbs, both in soluble and solid-phase form, gp90/Mac-2BP was able to down-regulate the proliferative response to anti-CD3 MoAb, whereas the response to anti-TCR alphabeta MoAb was enhanced. A similar differential effect was observed when a MoAb against CD5 (another member of the scavenger receptor superfamily) was added to anti-CD3 or anti-TCR-stimulated cells; anti-CD5 MoAb strongly down-modulated the CD3-mediated response, whereas its presence in culture was associated with potentiation of the response to TCR alphabeta agonists. gp90/Mac-2BP was able per se to up-regulate Ca2+ levels in freshly isolated lymphocytes; moreover, its presence in culture was associated with increased Ca2+ mobilization following stimulation with anti-TCR alphabeta, but not anti-CD3 MoAb. These data indicate that gp90/Mac-2BP could be able to influence some immune responses, possibly through multiple homologous interactions with other members of the scavenger receptor family; moreover, our findings suggest that signalling through the different components of the TCR:CD3 complex may follow distinct activation pathways into the cells.

Published
1998

32. Differential effect on TCR:CD3 stimulation of a 90-kD glycoprotein (gp90/Mac-2BP), a member of the scavenger receptor cysteine-rich domain protein family

Author

Silvestri, B, primary, Calderazzo, F, additional, Coppola, V, additional, Rosato, A, additional, Iacobelli, S, additional, Natoli, C, additional, Ullrich, A, additional, Sures, I, additional, Azam, M, additional, Brakebush, C, additional, Chieco-Bianchi, L, additional, and Amadori, A, additional

Published
1998
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33. In‐situ sol‐gel synthesis and characterization of bioactive pHEMA/SiO2 blend hybrids.

Author

Silvestri, B., Luciani, G., Costantini, A., Tescione, F., Branda, F., and Pezzella, A.

Subjects
POLYMER blends, GLASS transition temperature, SILICA gel, ADDITION polymerization, POLYMER colloids, MONOMERS
Abstract

A novel procedure to synthesize poly(2‐hydroxyethylmethacrylate)‐silica blend hybrids is presented. Methacrylate monomers bearing an alkoxysilyl unit, prepared by Michael addition of 2‐hydroxyethylmethacrylate (HEMA) to 3‐Aminopropyltriethoxysilane (APTS) were employed. By 13C NMR and mass analysis it was possible to establish the formation of coupling hybrid species. Hybrid materials, with final concentration ranging from 10% to 30% w/w of silica gel to the mass of polymer, were obtained through basic catalyzed sol‐gel process of tetraethoxysilane (TEOS) and the alkoxysilyl unit of the hybrid monomer, followed by in‐situ free‐radical polymerization. The hybrids were characterized as far as concerns their thermal properties (glass transition temperature, decomposition temperature), their sorption behavior in water, and in‐vitro bioactivity. Optical transparency, higher glass transition temperature, and higher decomposition temperature than pHEMA suggest an increase in either density or intensity of cross‐links between the organic and the inorganic phases. The swelling ratio of the 30% hybrids is comparable to pHEMA, whereas it is lower for the other compositions. In‐vitro bioactivity of the hybrids, due to the inorganic phase, was ascertained. Soaking time required for apatite deposition on the samples surface decreases as the content of silica gel increases. Therefore, the obtained bioactive hybrids can be used to make bioactive scaffolds for bone engineering. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009 [ABSTRACT FROM AUTHOR]

Published
2009
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36. ICT Services for Electro-Mobility

Author

Rinaldi, A., Amditis, A., Berardi, A., Fanti, M.P., Giansante, C., Launo, C., Mangini, A.M., Porru, S., Portouli, E., Roccotelli, M., Silvestri, B., Ukovich, W., and Winder, A.

Subjects
ICT services, smart mobility, electromobility, light electric vehicles, urban mobility, urban transport, 11. Sustainability
Abstract

The evolution of mobility in a sustainable perspective requires specific attention to the end-users roles and the changing needs of the cities and policies. This work shows how ICT (Information and Communications Technology) services can allow the transition towards smart and sustainable mobility. Within the European project H2020 ELVITEN (GA nr. 769926), a proper set of ICT services are described, including the brokering and the booking service for vehicles and charging stations, the ICT platform for monitoring the fleet of electric vehicles, the serious game app and a smart app for incentives. These services are suitable and indispensable to incentivize and encourage users towards a new mobility that is electric and sustainable.

38. Multicenter APULIA HF study: Efficacy of a shared management protocol between hospital and territory of patients hospitalized for acute heart failure,Studio multicentrico APULIA HF: Efficacia di un protocollo di gestione condiviso fra ospedale e territorio dei pazienti ospedalizzati per scompenso cardiaco acuto

Author

Iacoviello, M., Pede, S., Aspromonte, N., Bux, F., Panunzio, M., Donadeo, V., Castro, A., Antoncecchi, E., D Amato, N., Squiccimarro, E., Silvestri, B., Malerba, L., Gennaro, L., Modugno, G., Serafini, E., Curci, B., Grande, D., Furio Colivicchi, Luca, G., Ignone, G., D Agostino, C., and Caldarola, P.

41. ICT Services for Electro-Mobility

Author

Rinaldi, A., Amditis, A., Berardi, A., Fanti, M.P., Giansante, C., Launo, C., Mangini, A.M., Porru, S., Portouli, E., Roccotelli, M., Silvestri, B., Ukovich, W., and Winder, A.

Subjects
ICT services, smart mobility, electromobility, light electric vehicles, urban mobility, urban transport, 11. Sustainability
Abstract

The evolution of mobility in a sustainable perspective requires specific attention to the end-users roles and the changing needs of the cities and policies. This work shows how ICT (Information and Communications Technology) services can allow the transition towards smart and sustainable mobility. Within the European project H2020 ELVITEN (GA nr. 769926), a proper set of ICT services are described, including the brokering and the booking service for vehicles and charging stations, the ICT platform for monitoring the fleet of electric vehicles, the serious game app and a smart app for incentives. These services are suitable and indispensable to incentivize and encourage users towards a new mobility that is electric and sustainable.

44. Adding Humic Acids to Gelatin Hydrogels: A Way to Tune Gelation

Author

Virginia Venezia, Pietro Renato Avallone, Giuseppe Vitiello, Brigida Silvestri, Nino Grizzuti, Rossana Pasquino, Giuseppina Luciani, Venezia, V., Avallone, P. R., Vitiello, G., Silvestri, B., Grizzuti, N., Pasquino, R., and Luciani, G.

Subjects
Biomaterials, Polymers and Plastics, Humic Substance, Spectroscopy, Fourier Transform Infrared, Materials Chemistry, Gelatin, Water, Bioengineering, Hydrogels, Article, Humic Substances
Abstract

Exploring the chance to convert biowaste into a valuable resource, this study tests the potential role of humic acids (HA), a class of multifunctional compounds obtained by oxidative decomposition of biomass, as physical agents to improve gelatin's mechanical and thermal properties. To this purpose, gelatin-HA aqueous samples were prepared at increasing HA content. HA/gelatin concentrations changed in the range 2.67-26.67 (wt/wt)%. Multiple techniques were employed to assess the influence of HA content on the gel properties and to unveil the underlying mechanisms. HAs increased gel strength up to a concentration of 13.33 (wt/wt)% and led to a weaker gel at higher concentrations. FT-IR and DSC results proved that HAs can establish noncovalent interactions through H-bonding with gelatin. Coagulation phenomena occur because of HA-gelatin interactions, and at concentrations greater than 13.33 (wt/wt)%, HAs established preferential bonds with water molecules, preventing them from coordinating with gelatin chains. These features were accompanied by a change in the secondary structure of gelatin, which lost the triple helix structure and exhibited an increase in the random coil conformation. Besides, higher HA weight content caused swelling phenomena due to HA water absorption, contributing to a weaker gel. The current findings may be useful to enable a better control of gelatin structures modified with composted biowaste, extending their exploitation for a large set of technological applications.

Published
2021

45. Defects in the Amorphous–Crystalline Evolution of Gel-Derived TiO2

Author

Gian Paolo Papari, Can Koral, Antonella Rossi, Antonio Aronne, Claudio Imparato, Marzia Fantauzzi, Brigida Silvestri, Antonello Andreone, Koral, C., Fantauzzi, M., Imparato, C., Papari, G. P., Silvestri, B., Aronne, A., Andreone, A., and Rossi, A.

Subjects
Materials science, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Catalysis, Amorphous solid, Turn (biochemistry), General Energy, Chemical engineering, Physical and Theoretical Chemistry, 0210 nano-technology
Abstract

The catalytic and electro-optical properties of TiO2-based materials depend on the defectivity of their electronic structures and in turn on the synthesis conditions. It is however less clear how t...

Published
2020

46. Co-immobilization of Cellulase and β-Glucosidase into Mesoporous Silica Nanoparticles for the Hydrolysis of Cellulose Extracted from Eriobotrya japonica Leaves

Author

Giulio Pota, Antonio Sapienza Salerno, Aniello Costantini, Brigida Silvestri, Jessica Passaro, Valeria Califano, Pota, G., Sapienza Salerno, A., Costantini, A., Silvestri, B., Passaro, J., and Califano, V.

Subjects
Electrochemistry, General Materials Science, Surfaces and Interfaces, Condensed Matter Physics, Spectroscopy
Abstract

Fungal cellulases generally contain a reduced amount of β-glucosidase (BG), which does not allow for efficient cellulose hydrolysis. To address this issue, we implemented an easy co-immobilization procedure of β-glucosidase and cellulase by adsorption on wrinkled mesoporous silica nanoparticles with radial and hierarchical open pore structures, exhibiting smaller (WSN) and larger (WSN-p) inter-wrinkle distances. The immobilization was carried out separately on different vectors (WSN for BG and WSN-p for cellulase), simultaneously on the same vector (WSN-p), and sequentially on the same vector (WSN-p) in order to optimize the synergy between cellulase and BG. The obtained results pointed out that the best biocatalyst is that prepared through simultaneous immobilization of BG and cellulase on the same vector (WSN-p). In this case, the adsorption resulted in 20% yield of immobilization, corresponding to an enzyme loading of 100 mg/g of support. 82% yield of reaction and 72 μmol/min·g activity were obtained, evaluated for the hydrolysis of cellulose extracted from Eriobotrya japonica leaves. All reactions were carried out at a standard temperature of 50 °C. The biocatalyst retained 83% of the initial yield of reaction after 9 cycles of reuse. Moreover, it had better stability than the free enzyme mixture in a wide range of temperatures, preserving 72% of the initial yield of reaction up to 90 °C.

Published
2022

47. Surface acid properties of Nb2O5–P2O5–SiO2 gel-derived catalysts

Author

Gabriella Garbarino, Giovanni Pampararo, Elisabetta Finocchio, Guido Busca, Antonella Gervasini, Sebastiano Campisi, Brigida Silvestri, Claudio Imparato, Antonio Aronne, Garbarino, G., Pampararo, G., Finocchio, E., Busca, G., Gervasini, A., Campisi, S., Silvestri, B., Imparato, C., and Aronne, A.

Subjects
Settore CHIM/03 - Chimica Generale e Inorganica, History, Sol-gel, Polymers and Plastics, Niobia, Ethanol dehydration, General Chemistry, Phosphoric anhydride, Acid solids, Condensed Matter Physics, Settore CHIM/04 - Chimica Industriale, Industrial and Manufacturing Engineering, Mechanics of Materials, General Materials Science, Acid solids, Sol-gel, Ethanol dehydration, Niobia, Phosphoric anhydride, Business and International Management, Settore CHIM/02 - Chimica Fisica
Abstract

Amorphous catalytic materials belonging to the Nb2O5–P2O5–SiO2 system were prepared through an original sustainable sol-gel procedure, and characterized by surface area, porosity and acidity measurements. Their acid properties have been investigated by FT-IR spectroscopy of adsorbed pyridine, ammonia titration and TPR, as well as by titration with 2-phenylethylamine from organic solution. They present Lewis acidity attributed to niobium surface cationic centers, and Brønsted acidity sufficiently strong to protonate pyridine. The total acidity depends on the niobium content. The conversion of ethanol was investigated by vapor phase Temperature Programmed Reaction, steady-state catalytic experiments and static In Situ FTIR experiments. The samples are active in converting ethanol to ethylene with 100% selectivity at low conversion (523–573 K), but also produce acetaldehyde by dehydrogenation at higher temperature and conversion. They show an unusually poor activity for the production of diethylether also at low ethanol conversion. Ethoxy groups act as reaction surface intermediates.

Published
2022

48. Controlled Release of Doxorubicin for Targeted Chemo-Photothermal Therapy in Breast Cancer HS578T Cells Using Albumin Modified Hybrid Nanocarriers

Author

Barbara Carrese, Brigida Silvestri, Giulio Pota, Gennaro Sanità, Gaetano Calì, Luca Menichetti, Annalisa Lamberti, Chiara Cavallini, Paolo Armanetti, Giuseppina Luciani, Carrese, B., Cavallini, C., Sanita, G., Armanetti, P., Silvestri, B., Cali, G., Pota, G., Luciani, G., Menichetti, L., and Lamberti, A.

Subjects
Apoptosis, photothermal, melanin nanoparticles, Drug Delivery Systems, Tumor Cells, Cultured, Biology (General), Spectroscopy, media_common, Drug Carriers, Antibiotics, Antineoplastic, Chemistry, General Medicine, Controlled release, Combined Modality Therapy, Computer Science Applications, Drug delivery, Female, medicine.drug, Drug, PTT, QH301-705.5, Infrared Rays, Photothermal Therapy, media_common.quotation_subject, Breast Neoplasms, doxorubicin, Catalysis, Article, Inorganic Chemistry, hybrid nanoparticles, breast cancer, In vivo, Albumins, medicine, Humans, Doxorubicin, Physical and Theoretical Chemistry, Molecular Biology, QD1-999, Cell Proliferation, Organic Chemistry, hybrid nanoparticle, Photothermal therapy, melanin nanoparticle, Drug Liberation, drug delivery, Cancer research, Nanoparticles, Molecular imaging, Nanocarriers
Abstract

Hybrid nanomaterials have attracted research interest owing to their intriguing properties, which may offer new diagnostic options with triggering features, able to realize a new kind of tunable nanotherapeutics. Hybrid silica/melanin nanoparticles (NPs) containing silver seeds (Me-laSil_Ag-HSA NPs) disclosed relevant photoacoustic contrast for molecular imaging. In this study we explored therapeutic function in the same nanoplatform. For this purpose, MelaSil_Ag-HSA were loaded with doxorubicin (DOX) (MelaSil_Ag-HSA@DOX) and tested to assess the efficiency of drug delivery combined with concurrent photothermal treatment. The excellent photothermal properties allowed enhanced cytotoxic activity at significantly lower doses than neat chemotherapeutic treatment. The results revealed that MelaSil_Ag-HSA@DOX is a promising platform for an integrated photothermal (PT) chemotherapy approach, reducing the efficacy concentration of the DOX and, thus, potentially limiting the several adverse side effects of the drug in in vivo treatments.

Published
2021

49. Tailoring Structure: Current Design Strategies and Emerging Trends to Hierarchical Catalysts

Author

Virginia Venezia, Giulio Pota, Brigida Silvestri, Aniello Costantini, Giuseppe Vitiello, Giuseppina Luciani, Venezia, V., Pota, G., Silvestri, B., Costantini, A., Vitiello, G., and Luciani, G.

Subjects
environmental and energetic application, hybrid structure, inorganic structure, environment application, organic structure, hierarchical photocatalyst, template free structures, Physical and Theoretical Chemistry, Catalysis, General Environmental Science
Abstract

Nature mimicking implies the design of nanostructured materials, which can be assembled into a hierarchical structure, thus outperforming the features of the neat components because of their multiple length scale organization. This approach can be effectively exploited for the design of advanced photocatalysts with superior catalytic activity for energy and environment applications with considerable development in the recent six years. In this context, we propose a review on the state of the art for hierarchical photocatalyst production. Particularly, different synthesis strategies are presented, including template-free structuring, and organic, inorganic, and hybrid templating. Furthermore, emerging approaches based on hybrid and bio-waste templating are also highlighted. Finally, a critical comparison among available methods is carried out based on the envisaged application.

Published
2022

50. Small heat-shock protein HSPB3 promotes myogenesis by regulating the lamin B receptor

Author

Tiago, Tatiana, Hummel, Barbara, Morelli, Federica F, Basile, Valentina, Vinet, Jonathan, Galli, Veronica, Mediani, Laura, Antoniani, Francesco, Pomella, Silvia, Cassandri, Matteo, Garone, Maria Giovanna, Silvestri, Beatrice, Cimino, Marco, Cenacchi, Giovanna, Costa, Roberta, Mouly, Vincent, Poser, Ina, Yeger-Lotem, Esti, Rosa, Alessandro, Alberti, Simon, Rota, Rossella, Ben-Zvi, Anat, Sawarkar, Ritwick, Carra, Serena, UMRS974, Université Pierre et Marie Curie - Paris 6 (UPMC), Università degli Studi di Modena e Reggio Emilia (UNIMORE), Max Planck Institute of Immunobiology and Epigenetics (MPI-IE), Max-Planck-Gesellschaft, Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], University of Bologna, Centre de recherche en Myologie – U974 SU-INSERM, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Ben-Gurion University of the Negev (BGU), Technische Universität Dresden = Dresden University of Technology (TU Dresden), University of Cambridge [UK] (CAM), Centre de Recherche en Myologie, Tiago, Tatiana [0000-0001-9290-248X], Pomella, Silvia [0000-0002-7007-4238], Cenacchi, Giovanna [0000-0001-5824-3118], Costa, Roberta [0000-0002-2884-6716], Yeger-Lotem, Esti [0000-0002-8279-7898], Rosa, Alessandro [0000-0001-9999-7223], Alberti, Simon [0000-0003-4017-6505], Rota, Rossella [0000-0002-9408-7711], Ben-Zvi, Anat [0000-0003-2477-6519], Carra, Serena [0000-0003-0939-0140], Apollo - University of Cambridge Repository, Tiago T., Hummel B., Morelli F.F., Basile V., Vinet J., Galli V., Mediani L., Antoniani F., Pomella S., Cassandri M., Garone M.G., Silvestri B., Cimino M., Cenacchi G., Costa R., Mouly V., Poser I., Yeger-Lotem E., Rosa A., Alberti S., Rota R., Ben-Zvi A., Sawarkar R., and Carra S.

Subjects
Cell biology, Molecular biology, Receptors, Cytoplasmic and Nuclear, 13/106, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], HeLa Cell, Muscle Development, Transfection, Settore MED/05, Article, Cell Line, molecular biology, myogenesis, HSPB3, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, 14/19, Muscle, Skeletal, Heat-Shock Proteins, 45/91, QH573-671, Heat-Shock Protein, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, 96/63, 3. Good health, Heat-Shock Proteins, Small, 631/80, Cytology, 631/337, Human, HeLa Cells
Abstract

International audience; Abstract One of the critical events that regulates muscle cell differentiation is the replacement of the lamin B receptor (LBR)-tether with the lamin A/C (LMNA)-tether to remodel transcription and induce differentiation-specific genes. Here, we report that localization and activity of the LBR-tether are crucially dependent on the muscle-specific chaperone HSPB3 and that depletion of HSPB3 prevents muscle cell differentiation. We further show that HSPB3 binds to LBR in the nucleoplasm and maintains it in a dynamic state, thus promoting the transcription of myogenic genes, including the genes to remodel the extracellular matrix. Remarkably, HSPB3 overexpression alone is sufficient to induce the differentiation of two human muscle cell lines, LHCNM2 cells, and rhabdomyosarcoma cells. We also show that mutant R116P-HSPB3 from a myopathy patient with chromatin alterations and muscle fiber disorganization, forms nuclear aggregates that immobilize LBR. We find that R116P-HSPB3 is unable to induce myoblast differentiation and instead activates the unfolded protein response. We propose that HSPB3 is a specialized chaperone engaged in muscle cell differentiation and that dysfunctional HSPB3 causes neuromuscular disease by deregulating LBR.

Published
2021

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