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Mitochondrial genomes have provided significant insights into the evolution of several insects. A typical mitogenome contains 37 genes, and variations in gene order can indicate evolutionary relationships between species. In this study, we have assembled the first complete mitogenomes of Endecous chape and E. onthophagus and analyzed the phylogenetic implications for the Gryllidea infraorder. We performed DNA extractions and genome sequencing for both Endecous species. Subsequently, we searched for raw data in the Sequence Read Archive (SRA) in NCBI. Using the SRA data, we assembled the partial mitogenome of Dianemobius nigrofasciatus and annotated the protein-coding genes (PCGs) for nine species. Phylogenomic relationships were reconstructed using Maximum Likelihood (ML) and Bayesian Inference (BI), utilizing the PCGs from 49 Gryllidea species. The mitogenome lengths of E. chape and E. onthophagus are 16,266 bp and 16,023 bp, respectively, while D. nigrofasciatus has a length of 15,359 bp. Our results indicate that species within the infraorder exhibit four types of gene order arrangements that align with their phylogenetic relationships. Both phylogenomic trees displayed strong support, and the ML corroborated with the literature. Gryllidea species have significantly contributed to various fields, and studying their mitogenomes can provide valuable insights into this infraorder evolution.

Sperberacris, a new Neotropical genus of Amblytropidiini. Sperberacris muriciensis n. sp. (type species) from the municipality of Murici, State of Alagoas, Brazil is described. The new genus differs from other tribe congenera by the epiproct suboval, furculae divided into four long sclerotized lobes, cerci curved inward, apex truncated and sclerotized at the tip and convex bridge of the epiphallus with two large black sclerotized spots. The new species presents a diploid number of 2n = 23, X0♂/24, XX♀, and the karyotype is made up of telo/acrocentric chromosomes, with one dot-like B chromosome occurring in some nuclei. Morphological descriptions are provided along with illustrations emphasizing the most significant diagnostic features of external morphology and male genitalia. Information on type specimens, material examined, and distribution provided.

Karyotypes in the worldwide subfamily Oecanthinae show variations in diploid number, chromosome morphology, and sex-chromosome system. This study described the chromosome set and phylogenetic relationships of four Neotropical species, Oecanthus lineolatus, O. valensis, O. pallidus, and O. pictus. We used classical cytogenetics and Bayesian Inference for phylogenetic reconstruction, using the mitochondrial genes COI, 12S rRNA, and 16S rRNA; and analyzed the phylogenetic patterns of changes in chromosome numbers, using ChromEvol. We observed differences in chromosome number among species and two different sex-chromosome systems. Oecanthus pictus showed 2n = 21, X0♂/22, XX♀; O. lineolatus, 2n = 20, XY♂/XX♀; and O. valensis and O. pallidus, 2n = 18, XY♂/XX♀. The karyotype of Oecanthus was asymmetric, one group with large chromosomes and variation in heterochromatin distribution, and another with small acrocentric chromosomes. The phylogenetic tree recovered two main groups: one with the Palearctic species and another with species from different bioregions, but with low posterior probability. The Neotropical species grouped separately, O. valensis and O. pictus with Nearctic and Ethiopian species, and O. pallidus and O. lineolatus in another, well-supported clade. Together, the phylogenic and chromosome data suggest descending dysploidy events during the evolution of the group.

The objective of this study was to evaluate the potential of mesquite bean flour (Prosopis juliflora) as an energy ingredient in extruded diets for juvenile Nile tilapia (Oreochromis niloticus). Two experiments were carried out: the first consisted of a study to evaluate the chemical composition and digestibility of the energy and nutrients of MBF; the second consisted of a growth test, in which juveniles consumed diets containing different proportions of corn substitution by MBF, in which zootechnical, hematological, physiological, and metabolic variables were evaluated. It was observed that MBF has a chemical composition similar to corn, as well as the apparent digestibility of energy and nutrients. The higher sucrose/starch ratio of the ingredient stands out, as well as the difference in digestibility (p < 0.05) between the predominant carbohydrates 87.63 and 99.25% for starch and sucrose, respectively. In the growth assay, no difference was observed between zootechnical variables (p > 0.05), and sucrase and alkaline phosphatase activities were increased (p < 0.05), which was not observed for amylase and lipase (p > 0.05). The hematological variables did not change (p > 0.05). Metabolic variables indicate a reduction in gluconeogenesis from amino acids, as can be seen by the reduction in liver transaminase levels (ALT and AST) and glutamate dehydrogenase (GDH), as well as the greater availability of free amino acids in plasmas (p < 0.05). Thus, it can be said that MBF has a high nutritional value and can totally replace corn in diets for juvenile tilapia and the metabolic findings indicate a potential protein-sparing effect., (© 2021. The Author(s), under exclusive licence to Springer Nature B.V.)