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Your search keyword '"Silva, Saimon M."' showing total 26 results
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26 results on '"Silva, Saimon M."'

6. Wired for Success: Probing the Effect of Tissue-Engineered Neural Interface Substrates on Cell Viability

Author

Nascimento, Adriana Teixeira do, primary, Mendes, Alexandre X., additional, Duchi, Serena, additional, Duc, Daniela, additional, Aguilar, Lilith C., additional, Quigley, Anita F., additional, Kapsa, Robert M. I., additional, Nisbet, David R., additional, Stoddart, Paul R., additional, Silva, Saimon M., additional, and Moulton, Simon E., additional

Published
2024
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8. Lubricin (PRG-4) anti-fouling coating for surface-enhanced Raman spectroscopy biosensing: towards a hierarchical separation system for analysis of biofluids

Author

Han, Mingyu, primary, Silva, Saimon M., additional, Russo, Matthew J., additional, Desroches, Pauline E., additional, Lei, Weiwei, additional, Quigley, Anita F., additional, Kapsa, Robert M. I., additional, Moulton, Simon E., additional, Stoddart, Paul R., additional, and Greene, George W., additional

Published
2024
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12. A TISSUE-ENGINEERED NEURAL INTERFACE WITH PHOTOTHERMAL FUNCTIONALITY

Author

Nascimento, Adriana Teixeira do, primary, Mendes, Alexandre Xavier, additional, Begeng, James M., additional, Duchi, Serena, additional, Stoddart, Paul R., additional, Quigley, Anita F., additional, Kapsa, Robert M.I., additional, Ibbotson, Michael R., additional, Silva, Saimon M., additional, and Moulton, Simon E., additional

Published
2023
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13. Reagentless protein-based electrochemical biosensors.

Author

Silva, Saimon M., Li, Miaosi, Mendes, Alexandre Xavier, and Moulton, Simon E.

Subjects
*BIOSENSORS, *ELECTROCHEMICAL sensors, *DETECTORS
Abstract

The creation of reagentless protein-based biosensors that are capable of monitoring molecular analytes directly in bodily fluids could revolutionize our understanding of biology and personalized health monitoring. The limited number of molecular sensors that are currently available in the market depends on the specific enzymatic or chemical reactivity of their target analytes and therefore are not applicable to many relevant biomarkers. Aiming to overcome this limited molecular sensing generality, a new class of reagentless protein-based electrochemical sensors has been introduced for the direct measurements of biomarkers in unprocessed biological fluids. This mini-review will discuss the most recent cutting-edge discoveries for the development of electroanalytical modular biosensors, where all the sensors' components are integrated into a self-sufficient sensor allowing hence its autonomous functionality. [ABSTRACT FROM AUTHOR]

Published
2023
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16. Lubricin as a tool for controlling adhesion in vivo and ex vivo

Author

Manasa, Clayton S., primary, Silva, Saimon M., additional, Desroches, Pauline E., additional, Dennaoui, Jessair, additional, Russo, Mathew J., additional, Han, Mingyu, additional, Quigley, Anita F., additional, Greene, George W., additional, Kapsa, Robert M. I., additional, and Moulton, Simon E., additional

Published
2021
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21. Amperometric Electrochemical Platform for Hydrazine Determination Exploiting Reduced Graphene Oxide, Co(Salophen) and DNA: Application in Pharmaceutical Formulations Samples

Author

Freitas,Ludymila V., Lima,Kayni C. M. S., Silva,Saimon M., Leite,Fernando R. F., Fernandes,Ridvan N., Santos,Wallans T. P., Damos,Flavio S., and Luz,Rita C. S.

Subjects
sensor, pharmaceutical formulations, Co(Salophen), hydrazine, DNA, reduced graphene oxide
Abstract

The present work presents the development of a sensitive and selective amperometric sensor for the determination of hydrazine (HZ) in pharmaceutical formulations using a glassy carbon electrode (GCE) modified with a composite based on Co(Salophen), reduced graphene oxide (rGO) and deoxyribonucleic acid (DNA). The rGO/Co(Salophen)/DNA composite was characterized by using Fourier-transform infrared spectroscopy (FTIR), cyclic voltammetry, and amperometry. The proposed platform presented a well-defined voltammetric profile with a redox couple around 0.32 V vs. Ag/AgCl which showed excellent catalytic activity towards HZ oxidation. The peak current of HZ electrochemical oxidation on the proposed electrochemical platform have changed linearly with the HZ concentration in the range from 2 to 364 µmol L-1. The proposed platform presented sensitivity, limit of detection, and limit of quantification of 0.056 µA L µmol-1, 0.54 µmol L-1, and 1.64 µmol L-1 to HZ, respectively. The relative standard deviation for eight determinations using a solution of 50 µmol L-1 HZ was 0.85%. The proposed sensor was successfully applied for the determination of HZ in pharmaceutical formulations, and the recovery tests showed a good accuracy with recovery percentage between 99 and 101%.

Published
2018

26. Lubricin (PRG-4) anti-fouling coating for surface-enhanced Raman spectroscopy biosensing: towards a hierarchical separation system for analysis of biofluids.

Author

Han M, Silva SM, Russo MJ, Desroches PE, Lei W, Quigley AF, Kapsa RMI, Moulton SE, Stoddart PR, and Greene GW

Subjects
Humans, Spectrum Analysis, Raman methods, Glycoproteins, Biosensing Techniques methods, Biofouling prevention & control
Abstract

Surface-enhanced Raman Spectroscopy (SERS) is a powerful optical sensing technique that amplifies the signal generated by Raman scattering by many orders of magnitude. Although the extreme sensitivity of SERS enables an extremely low limit of detection, even down to single molecule levels, it is also a primary limitation of the technique due to its tendency to equally amplify 'noise' generated by non-specifically adsorbed molecules at (or near) SERS-active interfaces. Eliminating interference noise is thus critically important to SERS biosensing and typically involves onerous extraction/purification/washing procedures and/or heavy dilution of biofluid samples. Consequently, direct analysis within biofluid samples or in vivo environments is practically impossible. In this study, an anti-fouling coating of recombinant human Lubricin (LUB) was self-assembled onto AuNP-modified glass slides via a simple drop-casting method. A series of Raman spectra were collected using rhodamine 6G (R6G) as a model analyte, which was spiked into NaCl solution or unprocessed whole blood. Likewise, we demonstrate the same sensing system for the quantitative detection of L-cysteine spiked in undiluted milk. It was demonstrated for the first time that LUB coating can mitigate the deleterious effect of fouling in a SERS sensor without compromising the detection of a target analyte, even in a highly fouling, complex medium like whole blood or milk. This feat is achieved through a molecular sieving property of LUB that separates small analytes from large fouling species directly at the sensing interface resulting in SERS spectra with low background ( i.e. , noise) levels and excellent analyte spectral fidelity. These findings indicate the great potential for using LUB coatings together with an analyte-selective layer to form a hierarchical separation system for SERS sensing of relevant analytes directly in complex biological media, aquaculture, food matrix or environmental samples.

Published
2023
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