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Submitted by Laiane Souza da Silva (laiane.souza@hotmail.com) on 2019-08-10T05:50:21Z No. of bitstreams: 3 Carta_autodep??sito.pdf: 422496 bytes, checksum: 4c659e05305410877d5e17021d57b12b (MD5) AtaDefesa_1.pdf: 892980 bytes, checksum: 42f816a02a4c138699117b6473740335 (MD5) DISSERTA????O_LAIANE SOUZA.pdf: 2606912 bytes, checksum: 8450c5724adadb8b2db48183c6ce2808 (MD5) Approved for entry into archive by PPGCEM Ci??ncia e Engenharia de Materiais (ppgcem@ufam.edu.br) on 2019-08-10T09:21:51Z (GMT) No. of bitstreams: 3 Carta_autodep??sito.pdf: 422496 bytes, checksum: 4c659e05305410877d5e17021d57b12b (MD5) AtaDefesa_1.pdf: 892980 bytes, checksum: 42f816a02a4c138699117b6473740335 (MD5) DISSERTA????O_LAIANE SOUZA.pdf: 2606912 bytes, checksum: 8450c5724adadb8b2db48183c6ce2808 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2019-08-13T13:02:01Z (GMT) No. of bitstreams: 3 Carta_autodep??sito.pdf: 422496 bytes, checksum: 4c659e05305410877d5e17021d57b12b (MD5) AtaDefesa_1.pdf: 892980 bytes, checksum: 42f816a02a4c138699117b6473740335 (MD5) DISSERTA????O_LAIANE SOUZA.pdf: 2606912 bytes, checksum: 8450c5724adadb8b2db48183c6ce2808 (MD5) Made available in DSpace on 2019-08-13T13:02:01Z (GMT). No. of bitstreams: 3 Carta_autodep??sito.pdf: 422496 bytes, checksum: 4c659e05305410877d5e17021d57b12b (MD5) AtaDefesa_1.pdf: 892980 bytes, checksum: 42f816a02a4c138699117b6473740335 (MD5) DISSERTA????O_LAIANE SOUZA.pdf: 2606912 bytes, checksum: 8450c5724adadb8b2db48183c6ce2808 (MD5) Previous issue date: 2019-06-10 CAPES - Coordena????o de Aperfei??oamento de Pessoal de N??vel Superior With increasing of the resistance of microorganisms to commercial drugs, the search for new natural alternative antimicrobials, such as essential oils, has become im-portant. This research aims to test these essential oils against cutaneous pathogenic organisms that affect both humans and domestic animals, such as dogs and cats. Through the association with nanotechnology, this work proposes the encapsula-tion of these natural actives for the development of new formulations. Five natural active substances were selected: essential oil of Lippia alba, Lippia origanoides and Syzygium aromaticum; fixed oil of Dipteryx odorata and synthetic timol. The selected microorganisms were Sthaphylococcus aureus, Aspergillus fumigatus, Cryptococcus gattii, Candida albicans, Candida parapsilosis, Microsporum canis, Trichophyton mentalogrophytes, Trichophyton rubrum and Trichophyton tonsurans. For the devel-opment of new carrier materials, wall materials based on whey isolates, gums and phospholipids were selected to evaluate the interaction between the wall material and the active substances. The systems were submitted to the characterization techniques by NMR, UV-vis, DLS, FTIR, GC/MS and physico-chemical analysis. Among the tested substances, the essential oil of the specimens L. origanoides and S. aromaticum showed higher activity against the fungi and bacteria tested. The ac-tion can be attributed to the major constituents of oils, carvacrol, thymol and euge-nol, which are classified as phenolic compounds. Phenolic compounds have the activity of enzymatic catalysis of polyphenoloxidase, which is capable of oxidizing quinones, causing a toxic effect on microorganisms. Among the wall materials, whey isolates and phospholipid (soybean asolecitina) showed better interaction with the essential oils of the species S. aromaticum and L. origanoides when encapsu-lated. Both the protein and lipid wall materials exhibited satisfactory encapsulation efficiency. The systems that demonstrated better interaction and stability were WPI and Aso containing the thymol and MI conservants. This research may be consid-ered as the beginning of a developing process of a nanotecnological product based on natural actives for the control of the tested fungi and bacterium. Com aumento da resist??ncia dos micro-organismos ??s drogas comerciais, surge a busca por novos antimicrobianos alternativos naturais, como os ??leos essenciais. Esta pesquisa visa test??-los contra micro-organismos patog??nicos cut??neos que acometem tanto humanos como animais dom??sticos, como c??es e gatos. Atrav??s da associa????o com a nanotecnologia, este trabalho prop??e o encapsulamento desses ativos naturais para o desenvolvimento de novos sistemas. Foram selecionadas 5 subst??ncias naturais ativas: ??leo essencial de Lippia alba (erva cidreira), Lippia ori-ganoides (erva-de-Maraj??) e Syzygium aromaticum (cravo-da-??ndia); ??leo fixo de Dipteryx odorata (cumaru) e padr??o sint??tico timol. Os micro-organismos seleciona-dos foram Sthaphylococcus aureus, Aspergillus fumigatus, Cryptococcus gattii, Can-dida albicans, Candida parapsilosis, Microsporum canis, Trichophyton mentalo-grophytes, Trichophyton rubrum e Trichophyton tonsurans. Para o desenvolvimento de novos materiais carreadores foram selecionados materiais de parede baseados em isolados de soro de leite, gomas e fosfolip??dios, visando a avalia????o da intera????o entre o material de parede e as subst??ncias ativas testadas. Os sistemas foram submetidos ??s t??cnicas de caracteriza????o por RMN, UV-vis, DLS, FTIR, CG/EM e an??lises f??sico-qu??micas. Dentre as subst??ncias testadas, o ??leo essencial das es-p??cies L. origanoides e S. aromaticum apresentaram maior atividade contra os fun-gos e a bact??ria testados. A a????o pode ser atribu??da aos constituintes majorit??rios dos ??leos, carvacrol, timol e eugenol, que s??o classificados como compostos fen??li-cos. Os compostos fen??licos apresentam atividade de cat??lise enzim??tica de polife-noloxidase, que ?? capaz de oxidar a quinonas, causando efeito t??xico aos micro-organismos. Dentre os materiais de parede, os isolados do soro de leite e o fosfolip??-dio (asolecitina de soja) apresentaram melhor intera????o com os ??leos essenciais das esp??cies S. aromaticum e L. origanoides quando encapsulados. Tanto os mate-riais de parede proteicos quanto o lip??dico apresentaram efici??ncia de encapsula????o satisfat??ria. Os sistemas que demonstraram melhor intera????o e estabilidade foram os WPI e os Aso contendo o conservantes timol e MI. Acredita-se que esta pesquisa possa ser considerada como o in??cio de um processo de desenvolvimento de um produto nanotecnol??gico de controle alternativo dos fungos e bact??ria testados.