45 results on '"Silberstein, Lev"'
Search Results
2. VEGF-C protects the integrity of the bone marrow perivascular niche in mice
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Fang, Shentong, Chen, Shuo, Nurmi, Harri, Leppänen, Veli-Matti, Jeltsch, Michael, Scadden, David, Silberstein, Lev, Mikkola, Hanna, and Alitalo, Kari
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- 2020
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3. Characterisation of the SCL gene stem cell enhancer
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Silberstein, Lev
- Subjects
610 - Published
- 2005
4. Proximity-Based Differential Single-Cell Analysis of the Niche to Identify Stem/Progenitor Cell Regulators
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Silberstein, Lev, Goncalves, Kevin A., Kharchenko, Peter V., Turcotte, Raphael, Kfoury, Youmna, Mercier, Francois, Baryawno, Ninib, Severe, Nicolas, Bachand, Jacqueline, Spencer, Joel A., Papazian, Ani, Lee, Dongjun, Chitteti, Brahmananda Reddy, Srour, Edward F., Hoggatt, Jonathan, Tate, Tiffany, Lo Celso, Cristina, Ono, Noriaki, Nutt, Stephen, Heino, Jyrki, Sipilä, Kalle, Shioda, Toshihiro, Osawa, Masatake, Lin, Charles P., Hu, Guo-fu, and Scadden, David T.
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- 2016
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5. Angiogenin Promotes Hematopoietic Regeneration by Dichotomously Regulating Quiescence of Stem and Progenitor Cells
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Goncalves, Kevin A., Silberstein, Lev, Li, Shuping, Severe, Nicolas, Hu, Miaofen G., Yang, Hailing, Scadden, David T., and Hu, Guo-fu
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- 2016
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- View/download PDF
6. Ibrutinib response in a patient with refractory mixed essential cryoglobulinemia
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Jamali, Haya, primary, Wu, David, additional, Soma, Lorinda, additional, Linenberger, Michael, additional, Wener, Mark H., additional, and Silberstein, Lev, additional
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- 2023
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- View/download PDF
7. Myocardial Infarction Activates CCR2+ Hematopoietic Stem and Progenitor Cells
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Dutta, Partha, Sager, Hendrik B., Stengel, Kristy R., Naxerova, Kamila, Courties, Gabriel, Saez, Borja, Silberstein, Lev, Heidt, Timo, Sebas, Matthew, Sun, Yuan, Wojtkiewicz, Gregory, Feruglio, Paolo Fumene, King, Kevin, Baker, Joshua N., van der Laan, Anja M., Borodovsky, Anna, Fitzgerald, Kevin, Hulsmans, Maarten, Hoyer, Friedrich, Iwamoto, Yoshiko, Vinegoni, Claudio, Brown, Dennis, Di Carli, Marcelo, Libby, Peter, Hiebert, Scott W., Scadden, David T., Swirski, Filip K., Weissleder, Ralph, and Nahrendorf, Matthias
- Published
- 2015
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- View/download PDF
8. Contributors
- Author
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Abdel-Wahab, Omar, primary, Abrahm, Janet L., additional, Adams, Sharon, additional, Adewoye, Adeboye H., additional, Allen, Carl, additional, Ambinder, Richard F., additional, Anasetti, Claudio, additional, Anastasi, John, additional, Anderson, Julia A., additional, Antin, Joseph H., additional, Antony, Aśok C., additional, Araten, David J., additional, Armand, Philippe, additional, Armstrong, Gillian, additional, Armstrong, Scott A., additional, Arnold, Donald M., additional, Artz, Andrew S., additional, Awan, Farrukh T., additional, Baglin, Trevor P., additional, Benson, Don M., additional, Benz, Edward J., additional, Berliner, Nancy, additional, Bhagat, Govind, additional, Bhardwaj, Nina, additional, Bhatia, Ravi, additional, Bhatia, Smita, additional, Bhatt, Mihir D., additional, Bhatt, Vijaya Raj, additional, Bitan, Menachem, additional, Blinderman, Craig D., additional, Bollard, Catherine M., additional, Braun, Benjamin S., additional, Brenner, Malcolm K., additional, Brittenham, Gary M., additional, Brodsky, Robert A., additional, Brown, Myles, additional, Broxmeyer, Hal E., additional, Brummel-Ziedins, Kathleen, additional, Brunner, Andrew M., additional, Buadi, Francis K., additional, Burkhardt, Birgit, additional, Burns, Melissa, additional, Byrd, John C., additional, Caimi, Paolo F., additional, Caligiuri, Michael A., additional, Canavan, Michelle, additional, Cantor, Alan B., additional, Carcao, Manuel, additional, Carroll, Michael C., additional, Carty, Shannon A., additional, Castillo, Jorge J., additional, Chan, Anthony K.C., additional, Chapin, John, additional, Chiu, April, additional, Chute, John P., additional, Clark, David B., additional, Coates, Thomas D., additional, Cogle, Christopher R., additional, Connell, Nathan T., additional, Cooke, Elizabeth, additional, Cooley, Sarah, additional, Corradini, Paolo, additional, Creager, Mark A., additional, Creger, Richard J., additional, Cromwell, Caroline, additional, Crowther, Mark A., additional, Cushing, Melissa M., additional, Cutler, Corey, additional, Dang, Chi V., additional, Danial, Nika N., additional, Dave, Sandeep S., additional, DeCaprio, James A., additional, Dinauer, Mary C., additional, Dinner, Shira, additional, Diz-Küçükkaya, Reyhan, additional, Dodd, Roger Y., additional, Donato, Michele L., additional, Dorshkind, Kenneth, additional, Dotti, Gianpietro, additional, Dror, Yigal, additional, Dunleavy, Kieron, additional, Dvorak, Christopher C., additional, Ebert, Benjamin L., additional, Eck, Michael J., additional, Eikelboom, John W., additional, Epperla, Narendranath, additional, Ershler, William B., additional, Evans, William E., additional, Faderl, Stefan, additional, Ferrara, James L.M., additional, Filipovich, Alexandra Hult, additional, Fischer, Martin, additional, Fredenburgh, James C., additional, Friedman, Kenneth D., additional, Fuchs, Ephraim, additional, Fuller, Stephen J., additional, Gailani, David, additional, Galipeau, Jacques, additional, Gallagher, Patrick G., additional, Ganapathi, Karthik A., additional, Gardner, Lawrence B., additional, Gee, Adrian P., additional, Gerson, Stanton L., additional, Gertz, Morie A., additional, Giardina, Patricia J., additional, Gibson, Christopher J., additional, Golan, Karin, additional, Golub, Todd R., additional, Gonzales, Matthew J., additional, Gotlib, Jason, additional, Gottschalk, Stephen, additional, Grant, Marianne A., additional, Graubert, Timothy A., additional, Gregg, Xylina T., additional, Gribben, John G., additional, Gross, Dawn M., additional, Gruber, Tanja A., additional, Guitart, Joan, additional, Gurbuxani, Sandeep, additional, Gur-Cohen, Shiri, additional, Gutierrez, Alejandro, additional, Hamadani, Mehdi, additional, Hari, Parameswaran N., additional, Hartwig, John H., additional, Hayman, Suzanne R., additional, Hayward, Catherine P.M., additional, Hebbel, Robert P., additional, Heslop, Helen E., additional, Hillis, Christopher, additional, Hillyer, Christopher D., additional, Ho, Karin, additional, Hockenbery, David M., additional, Hoffman, Ronald, additional, Hogg, Kerstin E., additional, Holtan, Shernan G., additional, Horny, Hans-Peter, additional, Hsu, Yen-Michael S., additional, Hunter, Zachary R., additional, Huntington, James A., additional, Iancu-Rubin, Camelia, additional, Iqbal, Ali, additional, Isenman, David E., additional, Israels, Sara J., additional, Italiano, Joseph E., additional, Jaffe, Elaine S., additional, Jaffer, Iqbal H., additional, Jagannath, Sundar, additional, Jäger, Ulrich, additional, Jain, Nitin, additional, James, Paula, additional, Jeha, Sima, additional, Jordan, Michael B., additional, Josephson, Cassandra D., additional, Jung, Moonjung, additional, Kager, Leo, additional, Kambayashi, Taku, additional, Kanakry, Jennifer A., additional, Kantarjian, Hagop M., additional, Kaplan, Jason, additional, Karafin, Matthew S., additional, Karsan, Aly, additional, Kaufman, Randal J., additional, Kaufman, Richard M., additional, Keller, Frank G., additional, Kelly, Kara M., additional, Kessler, Craig M., additional, Key, Nigel S., additional, Keyzner, Alla, additional, Khandoga, Alexander G., additional, Khanna-Gupta, Arati, additional, Khatib-Massalha, Eman, additional, Klein, Harvey G., additional, Knoechel, Birgit, additional, Kollet, Orit, additional, Konkle, Barbara A., additional, Kontoyiannis, Dimitrios P., additional, Koreth, John, additional, Koretzky, Gary A., additional, Kotecha, Dipak, additional, Kremyanskaya, Marina, additional, Kumari, Anju, additional, Kuzel, Timothy M., additional, Küppers, Ralf, additional, Lacy, Martha Q., additional, Ladas, Elana, additional, Landier, Wendy, additional, Lapid, Kfir, additional, Lapidot, Tsvee, additional, Larson, Peter J., additional, Levi, Marcel, additional, Lewis, Russell E., additional, Liebman, Howard A., additional, Lillicrap, David, additional, Lim, Wendy, additional, Lin, Judith C., additional, Lindblad, Robert, additional, Lip, Gregory Y.H., additional, Little, Jane A., additional, Lohr, Jens G., additional, López, José A., additional, Luscinskas, Francis W., additional, Maciejewski, Jaroslaw P., additional, Majhail, Navneet S., additional, Manches, Olivier, additional, Mandle, Robert J., additional, Mann, Kenneth G., additional, Manno, Catherine S., additional, Marcogliese, Andrea N., additional, Mariani, Guglielmo, additional, Marincola, Francesco M., additional, Mascarenhas, John, additional, Massberg, Steffen, additional, McEver, Rodger P., additional, McGrath, Emer, additional, McKinney, Matthew S., additional, Mehta, Rohtesh S., additional, Mentzer, William C., additional, Merlini, Giampaolo, additional, Merryman, Reid, additional, Michel, Marc, additional, Migliaccio, Anna Rita, additional, Miller, Jeffrey S., additional, Mims, Martha P., additional, Mondoro, Traci Heath, additional, Moorehead, Paul, additional, Muniz, Luciana R., additional, Munshi, Nikhil C., additional, Najfeld, Vesna, additional, Nayak, Lalitha, additional, Nazy, Ishac, additional, Neff, Anne T., additional, Ness, Paul M., additional, Notarangelo, Luigi D., additional, O'Brien, Sarah H., additional, O'Connor, Owen A., additional, O'Donnell, Martin, additional, Olson, Amanda, additional, Orkin, Stuart H., additional, Pai, Menaka, additional, Pai, Sung-Yun, additional, Paidas, Michael, additional, Panch, Sandhya R., additional, Pande, Reena L., additional, Papayannopoulou, Thalia, additional, Parikh, Rahul, additional, Petersdorf, Effie W., additional, Peterson, Shane E., additional, Pittaluga, Stefania, additional, Ponce, Doris M., additional, Popolo, Laura, additional, Prchal, Josef T., additional, Pui, Ching-Hon, additional, Puigserver, Pere, additional, Rak, Janusz, additional, Ramos, Carlos A., additional, Rand, Jacob H., additional, Rand, Margaret L., additional, Rao, Dinesh S., additional, Ravandi, Farhad, additional, Rawlings, David J., additional, Reddy, Pavan, additional, Reding, Mark T., additional, Reiter, Andreas, additional, Rice, Lawrence, additional, Riese, Matthew J., additional, Ritchey, Arthur Kim, additional, Roberts, David J., additional, Roman, Elizabeth, additional, Rooney, Cliona M., additional, Rosen, Steven T., additional, Rosenthal, David S., additional, Rossmann, Marlies P., additional, Rot, Antal, additional, Rowley, Scott D., additional, Rubnitz, Jeffrey E., additional, Rydz, Natalia, additional, Salama, Mohamed E., additional, Sauk, Steven, additional, Saunthararajah, Yogen, additional, Savage, William, additional, Scadden, David, additional, Schaefer, Kristen G., additional, Schiffman, Fred, additional, Schneidewend, Robert, additional, Schrier, Stanley L., additional, Schuchman, Edward H., additional, Scullion, Bridget Fowler, additional, Selvaggi, Kathy J., additional, Senoo, Keitaro, additional, Shaheen, Montaser, additional, Shaz, Beth H., additional, Shelburne, Samuel A., additional, Shpall, Elizabeth J., additional, Shurin, Susan B., additional, Siegal, Deborah, additional, Silberstein, Leslie E., additional, Silberstein, Lev, additional, Silverstein, Roy L., additional, Sloan, Steven R., additional, Smith, Franklin O., additional, Smith, James W., additional, Smith, Katy, additional, Steensma, David P., additional, Steinberg, Martin H., additional, Stock, Wendy, additional, Storry, Jill R., additional, Stramer, Susan L., additional, Strauss, Ronald G., additional, Stroncek, David F., additional, Taylor, Justin, additional, Thota, Swapna, additional, Treon, Steven P., additional, Tulpule, Anil, additional, Valdes, Roberto Ferro, additional, Valent, Peter, additional, Vedantham, Suresh, additional, Vercellotti, Gregory M., additional, Verneris, Michael R., additional, Vichinsky, Elliott P., additional, von Andrian, Ulrich H., additional, Vose, Julie M., additional, Wagner, Andrew J., additional, Wang, Ena, additional, Wang, Jia-huai, additional, Warkentin, Theodore E., additional, Wasserstein, Melissa P., additional, Webster, Ann, additional, Weisdorf, Daniel J., additional, Weitz, Jeffrey I., additional, Westhoff, Connie M., additional, Wheeler, Allison P., additional, Widick, Page, additional, Wiley, James S., additional, William, Basem M., additional, Williams, David A., additional, Wilson, Wyndham H., additional, Wolfe, Joanne, additional, Wolgast, Lucia R., additional, Wood, Deborah, additional, Wu, Jennifer, additional, Yahalom, Joachim, additional, Yee, Donald L., additional, Younes, Anas, additional, Young, Neal S., additional, and Zeller, Michelle P., additional
- Published
- 2018
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9. Hematopoietic Microenvironment
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Scadden, David, primary and Silberstein, Lev, additional
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- 2018
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10. Chapter 14 - Hematopoietic Microenvironment
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Scadden, David and Silberstein, Lev
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- 2023
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11. In Vivo RNAi Screening Identifies a Leukemia-Specific Dependence on Integrin Beta 3 Signaling
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Miller, Peter G., Al-Shahrour, Fatima, Hartwell, Kimberly A., Chu, Lisa P., Järås, Marcus, Puram, Rishi V., Puissant, Alexandre, Callahan, Kevin P., Ashton, John, McConkey, Marie E., Poveromo, Luke P., Cowley, Glenn S., Kharas, Michael G., Labelle, Myriam, Shterental, Sebastian, Fujisaki, Joji, Silberstein, Lev, Alexe, Gabriela, Al-Hajj, Muhammad A., Shelton, Christopher A., Armstrong, Scott A., Root, David E., Scadden, David T., Hynes, Richard O., Mukherjee, Siddhartha, Stegmaier, Kimberly, Jordan, Craig T., and Ebert, Benjamin L.
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- 2013
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12. DAZL regulates Tet1 translation in murine embryonic stem cells
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Welling, Maaike, Chen, Hsu‐Hsin, Muñoz, Javier, Musheev, Michael U, Kester, Lennart, Junker, Jan Philipp, Mischerikow, Nikolai, Arbab, Mandana, Kuijk, Ewart, Silberstein, Lev, Kharchenko, Peter V, Geens, Mieke, Niehrs, Christof, van de Velde, Hilde, van Oudenaarden, Alexander, Heck, Albert JR, and Geijsen, Niels
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- 2015
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13. Myeloid-biased HSC require Semaphorin 4A from the bone marrow niche for self-renewal under stress and life-long persistence
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Toghani, Dorsa, primary, Zeng, Sharon, additional, Mahammadov, Elmir, additional, Crosse, Edie I., additional, Seyedhassantehrani, Negar, additional, Burns, Christian, additional, Gravano, David, additional, Radtke, Stefan, additional, Kiem, Hans-Peter, additional, Rodriguez, Sonia, additional, Carlesso, Nadia, additional, Pradeep, Amogh, additional, Wilson, Nicola K., additional, Kinston, Sarah J., additional, Gottgens, Berthold, additional, Nerlov, Claus, additional, Pietras, Eric, additional, Mesnieres, Marion, additional, Maes, Christa, additional, Kumanogoh, Atsushi, additional, Worzfeld, Thomas, additional, Kharchenko, Peter, additional, Scialdone, Antonio, additional, Spencer, Joel A, additional, and Silberstein, Lev, additional
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- 2022
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14. Differential stem- and progenitor-cell trafficking by prostaglandin [E.sub.2]
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Hoggatt, Jonathan, Mohammad, Khalid S., Singh, Pratibha, Hoggatt, Amber F., Chitteti, Brahmananda R., Speth, Jennifer M., Hu, Peirong, Poteat, Bradley A., Stilger, Kayla N., Ferraro, Francesca, Silberstein, Lev, Wong, Frankie K., Farag, Sherif S., Czader, Magdalena, Milne, Ginger L., Breyer, Richard M., Serezani, Carlos H., Scadden, David T., Guise, Theresa A., Srour, Edward F., and Pelus, Louis M.
- Subjects
Research ,Prostaglandins -- Research ,Hematopoietic stem cells -- Research - Abstract
To maintain lifelong production of blood cells, haematopoietic stem cells (HSCs) are tightly regulated by inherent programs and extrinsic regulatory signals received from their microenvironmental niche. Long-term repopulating HSCs reside [...]
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- 2013
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15. Myeloid-Biased HSC Require Semaphorin4a from the Bone Marrow Niche for Self-Renewal Under Stress and Life-Long Persistence
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Toghani, Dorsa, primary, Zeng, Sharon, additional, Mahammadov, Elmir, additional, Crosse, Edie, additional, Pradeep, Amogh, additional, Wilson, Nicola, additional, Kinston, Sarah, additional, Rodriguez, Sonia, additional, Seyedhassantehrani, Negar, additional, Gravano, David, additional, Burns, Christian, additional, Radtke, Stefan, additional, Carlesso, Nadia, additional, Kiem, Hans-Peter, additional, Scadden, David T., additional, Pietras, Eric, additional, Gottgens, Bertie, additional, Scialdone, Antonio, additional, Spencer, Joel, additional, and Silberstein, Lev, additional
- Published
- 2021
- Full Text
- View/download PDF
16. In vivo imaging of [T.sub.reg] cells providing immune privilege to the haematopoietic stem-cell niche
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Fujisaki, Joji, Wu, Juwell, Carlson, Alicia L., Silberstein, Lev, Putheti, Prabhakar, Larocca, Rafael, Gao, Wenda, Saito, Toshiki I., Celso, Cristina Lo, Tsuyuzaki, Hitoshi, Sato, Tatsuyuki, Cote, Daniel, Sykes, Megan, Strom, Terry B., Scadden, David T., and Lin, Charles P.
- Subjects
Research ,Properties ,Hematopoietic stem cells -- Properties -- Research ,Immunosuppression -- Research ,T cells -- Properties -- Research - Abstract
Despite rapid advances in our understanding of adult stem-cell biology, the immunological attributes of somatic stem-cell niches have remained largely unexplored. We hypothesized that the HSPC niche within the bone [...], Stem cells reside in a specialized regulatory microenvironment or niche (1,2), where they receive appropriate support for maintaining self-renewal and multi-lineage differentiation capacity (1-3). The niche may also protect stem cells from environmental insults (3) including cytotoxic chemotherapy and perhaps pathogenic immunity (4). The testis, hair follicle and placenta are all sites of residence for stem cells and are immune-suppressive environments, called immune-privileged sites, where multiple mechanisms cooperate to prevent immune attack, even enabling prolonged survival of foreign allografts without immunosuppression (4). We sought to determine if somatic stem-cell niches more broadly are immune-privileged sites by examining the haematopoietic stem/progenitor cell (HSPC) niche (1,2,5-7) in the bone marrow, a site where immune reactivity exists (8,9). We observed persistence of HSPCs from allogeneic donor mice (allo-HSPCs) in non-irradiated recipient mice for 30 days without immunosuppression with the same survival frequency compared to syngeneic HSPCs. These HSPCs were lost after the depletion of FoxP3 regulatory T ([T.sub.reg]) cells. High-resolution in vivo imaging over time demonstrated marked co-localization of HSPCs with [T.sub.reg] cells that accumulated on the endosteal surface in the calvarial and trabecular bone marrow. [T.sub.reg] cells seem to participate in creating a localized zone where HSPCs reside and where [T.sub.reg] cells are necessary for allo-HSPC persistence. In addition to processes supporting stem-cell function, the niche will provide a relative sanctuary from immune attack.
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- 2011
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17. B cells: fed and grown in the bone
- Author
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Silberstein, Lev, primary
- Published
- 2021
- Full Text
- View/download PDF
18. Contributors
- Author
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Abdel-Wahab, Omar, Abrahm, Janet L., Abutalib, Syed A., Adams, Sharon, Al Hadidi, Samer, Alcaide, Pilar, Allen, Carl E., Ambinder, Richard F., Amonoo, Hermioni L., Anderson, Julia A.M., Antin, Joseph H., Antony, Aśok C., Araten, David J., Arnold, Donald M., Awan, Farrukh T., Ayers, Emily C., Barth, Peter, Bates, Shannon M., Benz, Edward J., Berliner, Nancy, Bhardwaj, Nina, Bhatia, Smita, Bhatt, Mihir D., Bollard, Catherine M., Bowman, Mackenzie, Boyle, Eileen M., Braun, Benjamin S., Brenner, Malcolm K., Broccoli, Alessandro, Brodsky, Robert A., Brown, Myles, Broxmeyer, Hal E., Brummel-Ziedins, Kathleen, Brunner, Andrew M., Brunstein, Claudio G., Buadi, Francis K., Burkhardt, Birgit, Burns, Melissa A., Bussel, James B., Byrd, John C., Caimi, Paolo F., Camaschella, Clara, Canavan, Michelle, Cantor, Alan B., Capitano, Maegan L., Carcao, Manuel, Carrillo-Farga, Joaquin, Carroll, Michael C., Carson, William E., Carty, Shannon A., Castillo, Jorge J., Chakrabarti, Priyasmita, Chan, Anthony K.C., Chan, Noel C., Chhabra, Saurabh, Chute, John P., Cimen Bozkus, Cansu, Ciurea, Stefan O., Coates, Thomas D., Collins, Graham, Creager, Mark A., Cromwell, Caroline, Crowder, Lauren A., Cushing, Melissa M., Cutler, Corey, Dabaja, Bouthaina, Dave, Sandeep S., De, Caprio, James A., De, Carli, Kathryn, Deininger, Michael W., De, Simone, Robert A., Dinauer, Mary C., Dinner, Shira, Dixit, Karan S., Dogan, Ahmet, Dorshkind, Kenneth, Dotti, Gianpietro, Dror, Yigal, Dunleavy, Kieron, Dvorak, Christopher C., Ebert, Benjamin L., Eck, Michael J., Eckstein, Olive S., Eikelboom, John W., El Jamal, Siraj M., El-Jurdi, Najla, Kim El-Mallawany, Nader, Erba, Harry P., Evans, William E., Feld, Jonathan, Feldman, Alexander, Ferrara, James L., Fischer, Martin, Forbes, Lisa R., Franco, Idalid, Fredenburgh, James C., Frey, Noelle V., Friedman, Kenneth D., Fuller, Stephen J., Gabe, Caroline, Gailani, David, Gallagher, Patrick G., Gallardo-Grajeda, Andrea, Ganz, Tomas, Gee, Adrian P., George, Gemlyn, Gerson, Stanton L., Gertz, Morie A., Ghosh, Nilanjan, Gibson, Christopher J., Ginzburg, Yelena Z., Gomez, Keith, Gotlib, Jason, Grant, Shakira J., Graubert, Timothy A., Gregg, Xylina T., Gribben, John G., Gross, Peter L., Gulati, Nitya, Gurbuxani, Sandeep, Gutierrez, Alejandro, de Haan, Gerald, Haas-Kogan, Daphne, Haddad, Montaser, Halene, Stephanie, Hamadani, Mehdi, Hammond, Rebecca M., Hari, Parameswaran N., Hayman, Suzanne R., Hayward, Catherine, Hebbel, Robert P., Heidenreich, Olaf, Henkin, Stanislav, Hensch, Lisa, Hillis, Christopher, Hillyer, Christopher D., Hoffman, Ronald, Horny, Hans-Peter, Horowitz, Mia, Hsu, Katharine, Hub, Elin, Iancu-Rubin, Camelia, Isenman, David E., Israels, Sara J., Italiano, Joseph E., Jaffe, Elaine S., Jaffer, Iqbal H., Jäger, Ulrich, Jain, Nitin, James, Paula, Jimenez, Alexandra, Jordan, Michael B., Jung, Moonjung, Kager, Leo, Kanakry, Jennifer A., Kanate, Abraham S., Kelly, Kara M., Khandoga, Alexander G., Karsan, Aly, Kaufman, Richard M., Keyzner, Alla, Khanna-Gupta, Arati, Kharchenko, Evgeniya, Klein, Harvey G., Klepin, Heidi D., Anders Kolb, E., Kollet, Orit, Konopleva, Marina, Kontoyiannis, Dimitrios P., Koreth, John, Koretzky, Gary A., Kozieł-Siołkowska, Monika, Kremyanskaya, Marina, Kucine, Nicole, Kumar, Shaji, Küppers, Ralf, Lacy, Martha Q., Ola Landgren, C., Landier, Wendy, Lane, Andrew A., Lane, William J., Langer, Arielle L., Lapidot, Tsvee, Laureano, Marissa, Leblanc, Francis R., Le, Blanc, Thomas W., La, Casce, Ann S., Lee, Daniel W., Lee, Kathleen A., Leung, Wingchi, Levi, Marcel, Lewis, Russell E., Lichtman, Eben I., Lin, Judith C., Lin, Richard J., Lip, Gregory, Poh Loh, Kah, López, José A., Zoref Lorenz, Adi, Loughran, Thomas P., Lu, Sydney X., Lukas, Rimas V., Lulla, Premal, Luscinskas, Francis W., Ma, Alice D., Maakaron, Joseph E., Machlus, Kellie R., Maciejewski, Jaroslaw P., Magenau, John, Majhail, Navneet, Malek, Ehsan, Manis, John P., Mann, Kenneth G., Manno, Catherine S., Marcellino, Bridget K., Marcogliese, Andrea N., Marincola, Francesco M., Mascarenhas, John, Massberg, Steffen, Matino, Davide, Mc, Ever, Rodger P., Mc, Grath, Emer, Mc, Guire, Hilary, S. Mc, Kinney, Matthew, Mehta, Atul, A. Menapace, Laurel, Mentzer, William C., Michel, Marc, Rita Migliaccio, Anna, Mims, Martha P., Moffat, Karen A., Morgan, Gareth J., Najfeld, Vesna, Naymagon, Leonard, Ness, Paul M., Ng, Andrea K., Ning, Shuoyan, Notarangelo, Luigi D., O'Donnell, Martin, Ollila, Thomas A., Ordonez-Moreno, Alejandra, Own, Maryam, Pai, Menaka, Pai, Sung-Yun, Paidas, Michael, Papa, Luena, Papayannopoulou, Thalia, Peng, Cynthia S., Wang Petersdorf, Effie, Pickard, Lucy, Pierce, Glenn F., Pittaluga, Stefania, Porteus, Matthew, Potpara, Tatjana S., Prchal, Josef T., Puigserver, Pere, Vincent Rajkumar, S., Rak, Janusz, Ramos, Carlos A., Rand, Jacob H., Rand, Margaret L., Rao, Dinesh S., Rappazzo, Katherine C., Rau, Rachel E., Ravandi, Farhad, Rawley, Orla, Rawlings, David J., Reddy, Pavan, Reiter, Andreas, Reville, Barbara, Rice, Lawrence, Riese, Matthew J., Rippel, Noa, Riwes, Mary, Roberts, David J., Rodrigue, Bentley B., Rojek, Alexandra E., Roman, Elizabeth, Rossmann, Marlies P., Rot, Antal, Rouce, Rayne H., Rowley, Scott D., Rustad, Even H., Sahni, Gagan, Sahoo, Daisy, Salama, Mohamed E., Samji, Nasrin, Scadden, David, Schiffman, Fred J., Schipperus, Martin R., Schrier, Stanley L., Shaz, Beth H., Shelburne, Samuel A., Sheth, Sujit, Sholevar, Roxanne, Silberstein, Lev, Silverman, Lewis B., Silverstein, Roy L., Skeith, Leslie, Sloan, Steven R., Douglas Smith, Stephen, Steensma, David P., Steinberg, Martin H., Stieglitz, Elliot, Stock, Wendy, Storry, Jill R., Stramer, Susan L., Strati, Paolo, Stroncek, David F., Flohr Svendsen, Arthur, Sweetenham, John W., Tebaldi, Toma, Teegavarapu, Sravanti P., Tewari, Alok, Lay Thein, Swee, Tillman, Benjamin F., Tomlinson, Benjamin, Tremblay, Douglas, Treon, Steven P., Tsai, Frederick D., Tsang, Mazie, Tzannou, Ifigeneia, Usmani, Saad Z., Valent, Peter, Venkataraman, Girish, Venugopal, Sangeetha, M. Vercellotti, Gregory, Viero, Gabriella, von Andrian, Ulrich H., Vose, Julie M., Wagner, Andrew J., Wang, Ena, Wang, Jia-huai, Warkentin, Theodore E., Weitz, Jeffrey I., West, Kamille A., Westhoff, Connie M., Wheeler, Allison P., Wierda, William, Wiersum-Osselton, Johanna C., Wiley, James S., Williams, David A., Wolgast, Lucia R., Yang, Daozheng, Yang, Moua, Younes, Anas, Young, Neal S., Zayac, Adam S., Zeller, Michelle P., Zieske, Arthur W., Zimran, Ari, Luigi Zinzani, Pier, C. Zubair, Abba, and Michel Zwaan, C.
- Published
- 2023
- Full Text
- View/download PDF
19. Blood Scent
- Author
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Silberstein, Lev and Scadden, David T.
- Published
- 2013
- Full Text
- View/download PDF
20. Ascites and a raised serum Ca 125-confusing combination
- Author
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Silberstein, Lev B, Rosenthal, Adam N, Coppack, Simon W, Noonan, Kate, and Jacobs, Ian J
- Published
- 2001
21. Chapter 11 - Hematopoietic Microenvironment
- Author
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Scadden, David and Silberstein, Lev
- Published
- 2018
- Full Text
- View/download PDF
22. Cell Cycle Analysis of Hematopoietic Stem and Progenitor Cells by Multicolor Flow Cytometry
- Author
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Galvin, Amy, primary, Weglarz, Meredith, additional, Folz-Donahue, Kat, additional, Handley, Maris, additional, Baum, Misa, additional, Mazzola, Michael, additional, Litwa, Hannah, additional, Scadden, David T., additional, and Silberstein, Lev, additional
- Published
- 2018
- Full Text
- View/download PDF
23. Image-guided transplantation of single cells in the bone marrow of live animals
- Author
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Turcotte, Raphaël, primary, Alt, Clemens, additional, Runnels, Judith M., additional, Ito, Kyoko, additional, Wu, Juwell W., additional, Zaher, Walid, additional, Mortensen, Luke J., additional, Silberstein, Lev, additional, Côté, Daniel C., additional, Kung, Andrew L., additional, Ito, Keisuke, additional, and Lin, Charles P., additional
- Published
- 2017
- Full Text
- View/download PDF
24. Faculty Opinions recommendation of Hematopoietic Stem Cells Count and Remember Self-Renewal Divisions.
- Author
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Silberstein, Lev, primary
- Published
- 2017
- Full Text
- View/download PDF
25. Angiogenin Is a Niche-Secreted RNase Which Regulates Hematopoietic Regeneration
- Author
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Silberstein, Lev, primary, Goncalves, Kevin, additional, Severe, Nicholas, additional, Hu, Guo-fu, additional, and Scadden, David T., additional
- Published
- 2016
- Full Text
- View/download PDF
26. Proximity-based analysis of the bone marrow niche identifies novel extrinsic regulators of hematopoietic regeneration
- Author
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Silberstein, Lev, primary, Goncalves, Kevin, additional, Kharchenko, Peter, additional, Turcotte, Raphael, additional, Kfoury, Youmna, additional, Mercier, Francois, additional, Severe, Nicolas, additional, Baryawno, Ninib, additional, Lin, Charles, additional, and Scadden, David, additional
- Published
- 2016
- Full Text
- View/download PDF
27. Chapter 9 - Hematopoietic Microenvironment
- Author
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Silberstein, Lev and Scadden, David
- Published
- 2013
- Full Text
- View/download PDF
28. DAZL regulates Tet1 translation in murine embryonic stem cells
- Author
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Biomolecular Mass Spectrometry and Proteomics, Sub Biomol.Mass Spectrometry & Proteom., Sub Biomol.Mass Spect. and Proteomics, Faculty of Veterinary Medicine Research Groups, CSCA TR1, Welling, Maaike, Chen, Hsu Hsin, Munoz Peralta, Javier, Musheev, Michael U., Kester, Lennart, Junker, Jan Philipp, Mischerikow, Nikolai, Arbab, Mandana, Kuijk, Ewart, Silberstein, Lev, Kharchenko, Peter V., Geens, Mieke, Niehrs, Christof, Van De Velde, Hilde, Van Oudenaarden, Alexander, Heck, Albert J R, Geijsen, Niels, Biomolecular Mass Spectrometry and Proteomics, Sub Biomol.Mass Spectrometry & Proteom., Sub Biomol.Mass Spect. and Proteomics, Faculty of Veterinary Medicine Research Groups, CSCA TR1, Welling, Maaike, Chen, Hsu Hsin, Munoz Peralta, Javier, Musheev, Michael U., Kester, Lennart, Junker, Jan Philipp, Mischerikow, Nikolai, Arbab, Mandana, Kuijk, Ewart, Silberstein, Lev, Kharchenko, Peter V., Geens, Mieke, Niehrs, Christof, Van De Velde, Hilde, Van Oudenaarden, Alexander, Heck, Albert J R, and Geijsen, Niels
- Published
- 2015
29. DAZL regulates Tet1 translation in murine embryonic stem cells
- Author
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CMM Groep Cuppen, CMM Sectie Molecular Cancer Research, Hubrecht Institute with UMC, Cancer, Welling, Maaike, Chen, Hsu-Hsin, Muñoz, Javier, Musheev, Michael U, Kester, Lennart, Junker, Jan Philipp, Mischerikow, Nikolai, Arbab, Mandana, Kuijk, Ewart, Silberstein, Lev, Kharchenko, Peter V, Geens, Mieke, Niehrs, Christof, van de Velde, Hilde, van Oudenaarden, Alexander, Heck, Albert J R, Geijsen, Niels, CMM Groep Cuppen, CMM Sectie Molecular Cancer Research, Hubrecht Institute with UMC, Cancer, Welling, Maaike, Chen, Hsu-Hsin, Muñoz, Javier, Musheev, Michael U, Kester, Lennart, Junker, Jan Philipp, Mischerikow, Nikolai, Arbab, Mandana, Kuijk, Ewart, Silberstein, Lev, Kharchenko, Peter V, Geens, Mieke, Niehrs, Christof, van de Velde, Hilde, van Oudenaarden, Alexander, Heck, Albert J R, and Geijsen, Niels
- Published
- 2015
30. In Vivo RNAi Screening Identifies a Leukemia-Specific Dependence on Integrin Beta 3 Signaling
- Author
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Massachusetts Institute of Technology. Department of Biology, Koch Institute for Integrative Cancer Research at MIT, Hynes, Richard O., Labelle, Myriam, Miller, Peter G., Al-Shahrour, Fatima, Hartwell, Kimberly A., Chu, Lisa P., Jaras, Marcus, Puram, Rishi V., Puissant, Alexandre, Callahan, Kevin P., Ashton, John, McConkey, Marie, Poveromo, Luke P., Cowley, Glenn S., Kharas, Michael G., Shterental, Sebastian, Fujisaki, Joji, Silberstein, Lev, Alexe, Gabriela, Al-Hajj, Muhammad A., Shelton, Christopher A., Armstrong, Scott A., Root, David E., Scadden, David T., Mukherjee, Siddhartha, Stegmaier, Kimberly, Jordan, Craig T., Ebert, Benjamin L., Hynes, Richard O, Massachusetts Institute of Technology. Department of Biology, Koch Institute for Integrative Cancer Research at MIT, Hynes, Richard O., Labelle, Myriam, Miller, Peter G., Al-Shahrour, Fatima, Hartwell, Kimberly A., Chu, Lisa P., Jaras, Marcus, Puram, Rishi V., Puissant, Alexandre, Callahan, Kevin P., Ashton, John, McConkey, Marie, Poveromo, Luke P., Cowley, Glenn S., Kharas, Michael G., Shterental, Sebastian, Fujisaki, Joji, Silberstein, Lev, Alexe, Gabriela, Al-Hajj, Muhammad A., Shelton, Christopher A., Armstrong, Scott A., Root, David E., Scadden, David T., Mukherjee, Siddhartha, Stegmaier, Kimberly, Jordan, Craig T., Ebert, Benjamin L., and Hynes, Richard O
- Abstract
We used an in vivo small hairpin RNA (shRNA) screening approach to identify genes that are essential for MLL-AF9 acute myeloid leukemia (AML). We found that Integrin Beta 3 (Itgb3) is essential for murine leukemia cells in vivo and for human leukemia cells in xenotransplantation studies. In leukemia cells, Itgb3 knockdown impaired homing, downregulated LSC transcriptional programs, and induced differentiation via the intracellular kinase Syk. In contrast, loss of Itgb3 in normal hematopoietic stem and progenitor cells did not affect engraftment, reconstitution, or differentiation. Finally, using an Itgb3 knockout mouse model, we confirmed that Itgb3 is dispensable for normal hematopoiesis but is required for leukemogenesis. Our results establish the significance of the Itgb3 signaling pathway as a potential therapeutic target in AML., National Institutes of Health (U.S.) (Harvard Stem Cell Institute. GlaxoSmithKline. Grant P01 CA108631), National Institutes of Health (U.S.) (Harvard Stem Cell Institute. GlaxoSmithKline. Grant RC1 CA145229), National Institutes of Health (U.S.) (Harvard Stem Cell Institute. GlaxoSmithKline. Grant R01 CA140292), National Institutes of Health (U.S.) (Harvard Stem Cell Institute. GlaxoSmithKline. Grant CA148180)
- Published
- 2015
31. Embigin Regulates HSPC Homing and Quiescence and Acts As a Cell Surface Marker for a Niche Factor-Enriched Subset of Osteolineage Cells
- Author
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Silberstein, Lev, primary, Kharchenko, Peter V., additional, Kfoury, Youmna Sami, additional, Mercier, Francois, additional, Turcotte, Raphael, additional, Lin, Charles P., additional, Baryawno, Ninib, additional, Severe, Nicolas, additional, Tate, Tiffany, additional, Hoggatt, Jonathan, additional, and Scadden, David T., additional
- Published
- 2015
- Full Text
- View/download PDF
32. Proximity-Based Single Cell Analysis of the Bone Marrow Niche Identifies Interleukin-18 As a Quiescence Regulator of Early Hematopoietic Progenitors
- Author
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Silberstein, Lev, primary, Kharchenko, Peter, additional, Kfoury, Youmna, additional, Mercier, Francois, additional, Osawa, Masatake, additional, Hoggatt, Jonathan, additional, Tate, Tiffany, additional, Lin, Charles, additional, and Scadden, David T., additional
- Published
- 2014
- Full Text
- View/download PDF
33. Bayesian approach to single-cell differential expression analysis
- Author
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Kharchenko, Peter V, primary, Silberstein, Lev, additional, and Scadden, David T, additional
- Published
- 2014
- Full Text
- View/download PDF
34. Transgenic analysis of the stem cell leukemia +19 stem cell enhancer in adult and embryonic hematopoietic and endothelial cells
- Author
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Silberstein, Lev, Sánchez, María José, Socolovsky, Merav, Liu, Ying, Hoffman, Gary, Kinston, Sarah, Piltz, Sandie, Bowen, Mark, Gambardella, Laure, Green, Anthony R., Göttgens, Berthold, Silberstein, Lev, Sánchez, María José, Socolovsky, Merav, Liu, Ying, Hoffman, Gary, Kinston, Sarah, Piltz, Sandie, Bowen, Mark, Gambardella, Laure, Green, Anthony R., and Göttgens, Berthold
- Abstract
Appropriate transcriptional regulation is critical for the biological functions of many key regulatory genes, including the stem cell leukemia (SCL) gene. As part of a systematic dissection of SCL transcriptional regulation, we have previously identified a 5,245-bp SCL +18/19 enhancer that targeted embryonic endothelium together with embryonic and adult hematopoietic progenitors and stem cells (HSCs). This enhancer is proving to be a powerful tool for manipulating hematopoietic progenitors and stem cells, but the design and interpretation of such transgenic studies require a detailed understanding of enhancer activity in vivo. In this study, we demonstrate that the +18/19 enhancer is active in mast cells, megakaryocytes, and adult endothelium. A 644-bp +19 core enhancer exhibited similar temporal and spatial activity to the 5,245-bp +18/19 fragment both during development and in adult mice. Unlike the +18/19 enhancer, the +19 core enhancer was only active in adult mice when linked to the eukaryotic reporter gene human placental alkaline phosphatase. Activity of a single core enhancer in HSCs, endothelium, mast cells, and megakaryocytes suggests possible overlaps in their respective transcriptional programs. Moreover, activity in a proportion of thymocytes and other SCL-negative cell types suggests the existence of a silencer elsewhere in the SCL locus.
- Published
- 2005
35. Real-Time RT-PCR Analysis of Individual Osteolineage Cells within the Hematopoietic Stem Cell Niche
- Author
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Silberstein, Lev, primary, Osawa, Masatake, additional, Lin, Charles, additional, Kharchenko, Peter, additional, Celso, Cristina Lo, additional, Aribeana, Chiaka, additional, and Scadden, David T., additional
- Published
- 2011
- Full Text
- View/download PDF
36. Transcriptional Link between Blood and Bone: the Stem Cell Leukemia Gene and Its +19 Stem Cell Enhancer Are Active in Bone Cells
- Author
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Pimanda, John E., primary, Silberstein, Lev, additional, Dominici, Massimo, additional, Dekel, Benjamin, additional, Bowen, Mark, additional, Oldham, Scott, additional, Kallianpur, Asha, additional, Brandt, Stephen J., additional, Tannahill, David, additional, Göttgens, Berthold, additional, and Green, Anthony R., additional
- Published
- 2006
- Full Text
- View/download PDF
37. Transgenic Analysis of the Stem Cell Leukemia +19 Stem Cell Enhancer in Adult and Embryonic Hematopoietic and Endothelial Cells
- Author
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Silberstein, Lev, primary, Sánchez, María‐José, additional, Socolovsky, Merav, additional, Liu, Ying, additional, Hoffman, Gary, additional, Kinston, Sarah, additional, Piltz, Sandie, additional, Bowen, Mark, additional, Gambardella, Laure, additional, Green, Anthony R., additional, and Göttgens, Berthold, additional
- Published
- 2005
- Full Text
- View/download PDF
38. An SCL +19 Core Enhancer Targets Three Mesoderm-Derived Cell Lineages - Blood, Endothelium and Smooth Muscle.
- Author
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Silberstein, Lev, primary, Sanchez, Maria Jose, additional, Socolovsky, Merav, additional, Hoffman, Gary J., additional, Piltz, Sandie, additional, Bowen, Mark, additional, Green, Anthony R., additional, and Gottgens, Berthold, additional
- Published
- 2004
- Full Text
- View/download PDF
39. A Role for SCL in Developing Bone? the SCL +19 Core Enhancer Targets Expression to Cells of Developing Bone, Osteocytes, Chondrocytes and Bone Lining Cells.
- Author
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Silberstein, Lev, primary, Pimanda, John E., additional, Piltz, Sandie, additional, Delaney, Liz, additional, Oldham, Scott, additional, Kallianpur, Asha, additional, Dominici, Massimo, additional, Brandt, Stephen, additional, Gottgens, Berthold, additional, and Green, Anthony, additional
- Published
- 2004
- Full Text
- View/download PDF
40. Contributors
- Author
-
Abrahm, Janet L., Abrams, Charles S., Abrams, Donald I., Ackerman, Steven J., Adams, Sharon, Adewoye, Adeboye H., Allen, Carl, Ambinder, Richard F., Anasetti, Claudio, Anastasi, John, Anderson, Julia A., Andreeff, Michael, Antin, Joseph H., Antony, Aśok C., Apostolakis, Stavros, Armstrong, Scott A., Arnold, Donald M., Artz, Andrew S., Awan, Farrukh T., Banchereau, Jacques, Barker, Juliet N., Baum, Linda G., Benson, Don M., Jr., Benz, Edward J., Jr., Berliner, Nancy, Bhagat, Govind, Bhalla, Kapil N., Bhardwaj, Nina, Bhatia, Ravi, Bhatia, Smita, Blinderman, Craig D., Bollard, Catherine M., Brenner, Malcolm K., Brittenham, Gary M., Brodsky, Robert A., Broxmeyer, Hal E., Brummel-Ziedins, Kathleen, Buadi, Francis K., Butterfield, Joseph H., Byrd, John C., Caimi, Paolo F., Caligiuri, Michael A., Campagnaro, Erica, Canaani, Jonathan, Canavan, Michelle, Cantor, Alan B., Carcao, Manuel, Carroll, Michael C., Carty, Shannon A., Champlin, Richard E., Chan, Anthony K.C., Choate, Jacquelyn D., Chung, Peter, Chute, John P., Cines, Douglas B., Clark, David B., Coates, Thomas D., Cogle, Christopher R., Connell, Nathan T., Cooke, Elizabeth, Cooley, Sarah, Corradini, Paolo, Creager, Mark A., Creger, Richard J., Cromwell, Caroline, Cunningham, Regina S., Cushing, Melissa M., Cutler, Corey, Dahl, Gary V., Dang, Chi V., Danial, Nika N., Dave, Sandeep S., DeAngelo, Daniel J., Desai, Madhav V., Dey, Bimalangshu R., Diehl, Volker, Dinauer, Mary C., Diz-Küçükkaya, Reyhan, Donato, Michele L., Dorshkind, Kenneth, Dotti, Gianpietro, Dror, Yigal, Dunleavy, Kieron, Ebert, Benjamin L., Eck, Michael J., Eichenauer, Dennis A., Eikelboom, John W., Engert, Andreas, Ershler, William B., Esmon, Charles T., Esmon, Naomi L., Evans, William E., Faderl, Stefan, Ferrara, James L.M., Filipovich, Alexandra Hult, Freedman, Melvin H., Fuller, Stephen J., Gailani, David, Gallagher, Patrick G., Gardner, Lawrence B., Gee, Adrian P., Gerson, Stanton L., Gertz, Morie A., Giardina, Patricia J., Golan, Karin, Golub, Todd R., Gottschalk, Stephen, Grant, Steven, Green, David L., Gribben, John G., Guitart, Joan, Gur-Cohen, Shiri, Gurbuxani, Sandeep, Gutierrez, Alejandro, Hari, Parameswaran, Harlan, John M., Hartwig, John H., Hayman, Suzanne R., Hayward, Catherine P.M., Hebbel, Robert P., Heslop, Helen E., Hillyer, Christopher D., Hockenbery, David M., Hoffman, Ronald, Horowitz, Mary, Horwitz, Edwin M., Hromas, Robert A., Huang, Franklin W., Isenman, David E., Italiano, Joseph E., Jr., Jaffe, Elaine S., Jagannath, Sundar, Jäger, Ulrich, Jain, Nitin, James, Paula, Jeha, Sima, Jordan, Michael B., Josephson, Cassandra, Jung, Moonjung, Kager, Leo, Kamdar, Kala Y., Kanakry, Jennifer A., Kantarjian, Hagop M., Karafin, Matthew S., Karsan, Aly, Katz, Louis M., Kaufman, Randal J., Kaufman, Richard M., Keller, Frank G., Kelly, Kara M., Kelton, John, Kessler, Craig M., Key, Nigel S., Khandoga, Alexander G., Khanna-Gupta, Arati, Klein, Harvey G., Kollet, Orit, Konkle, Barbara A., Kontoyiannis, Dimitrios P., Koreth, John, Koretzky, Gary A., Kremyanskaya, Marina, Küppers, Ralf, Kuzel, Timothy M., Kwak, Larry W., Lakshmanan, Viswanathan, Landier, Wendy, Lapid, Kfir, Lapidot, Tsvee, Larson, Peter J., Lechner, Klaus, Lee, Andrea, Lee, William M.F., Levi, Marcel, Lewis, Russell E., Liebman, Howard A., Lillicrap, David, Lim, Wendy, Lin, Thomas S., Lindblad, Robert, Lip, Gregory Y.H., Little, Jane A., Loh, Mignon L., Look, A. Thomas, López, José A., Luscinskas, Francis W., Macartney, Christine A., Maciejewski, Jaroslaw P., Maitta, Robert W., Majhail, Navneet S., Manches, Olivier, Mandle, Robert, Mann, Kenneth G., Manno, Catherine S., Marchi, Enrica, Mariani, Guglielmo, Marincola, Francesco M., Marks, Peter W., Mascarenhas, John, Massberg, Steffen, Mauch, Peter M., McCorkle, Ruth, McCrae, Keith R., McEver, Rodger P., McGrath, Emer, McKinney, Matthew S., Meacham, Amy, Menitove, Jay E., Merlini, Giampaolo, Migliaccio, Anna Rita, Miller, Jeffrey S., Mims, Martha P., Mondoro, Traci Heath, Moorehead, Paul, Munshi, Nikhil C., Najfeld, Vesna, Nazi, Ishac, Neff, Anne T., Ness, Paul M., Ng, Andrea K., Notarangelo, Luigi D., O'Brien, Sarah H., O'Connor, Owen A., Donghaile, Diarmaid Ó, O'Donnell, Martin, Otis, Stavroula, Ou, Zhishuo, Pai, Sung-Yun, Palucka, Karolina, Pande, Reena L., Papayannopoulou, Thalia, Pardanani, Animesh, Paredes, Nethnapha, Patriquin, Christopher, Petersdorf, Effie W., Pittaluga, Stefania, Plow, Edward F., Ponce, Doris M., Popolo, Laura, Powell, Leland D., Price, Elizabeth A., Pui, Ching-Hon, Puigserver, Pere, Quintás-Cardama, Alfonso, Rak, Janusz, Ramos, Carlos A., Rand, Jacob H., Ravandi, Farhad, Rawlings, David J., Reddy, Pavan, Reding, Mark T., Rhee, Charles, Rice, Lawrence, Riese, Matthew J., Ritchey, Arthur Kim, Rivella, Stefano, Roberts, David J., Romaguera, Jorge E., Roman, Elizabeth, Rooney, Cliona M., Rosen, Steven T., Rosenthal, David S., Rosovsky, Rachel, Rowley, Scott D., Rydz, Natalia, Sadler, J. Evan, Sandlund, John T., Jr., Sauk, Steven, Saunthararajah, Yogen, Scadden, David, Schaefer, Kristen G., Schiffman, Fred J., Schmaier, Alvin H., Schrier, Stanley L., Schuchman, Edward H., Scullion, Bridget Fowler, Selvaggi, Kathy J., Shaheen, Montaser, Shaz, Beth H., Sheehan, Andrea M., Shelburne, Samuel A., Shlomchik, Mark J., Shurin, Susan B., Silberstein, Leslie E., Silberstein, Lev, Silverstein, Roy L., Sloan, Steven R., Smith, Franklin O., Smith, James, Snyder, Edward L., Soff, Gerald A., Spitzer, Thomas R., Steinberg, Martin H., Stock, Wendy, Stone, Richard M., Storry, Jill R., Strauss, Ronald G., Stroncek, David F., Szczepiorkowski, Zbigniew M., Tiu, Ramon V., Toltl, Lisa J., Toms, Angela, Tormey, Christopher A., Treon, Steven P., Tulpule, Anil, Vedantham, Suresh, Verneris, Michael R., Vichinsky, Elliott P., von Andrian, Ulrich H., Wagner, Andrew J., Wang, Ena, Wang, Jia-huai, Wang, Michael, Warkentin, Theodore E., Wasserstein, Melissa P., Wei, Michael C., Weinstein, Howard J., Weisdorf, Daniel J., Weitz, Jeffrey I., Westhoff, Connie M., Wiley, James S., Williams, David A., Wilson, Wyndham H., Wolfe, Joanne, Wolgast, Lucia R., Wood, Deborah, Wu, YanYun, Yee, Donald L., Young, Ken H., Young, Neal S., Zeldenrust, Steven R., Zhang, Liang, and Zhou, Ming-Ming
- Published
- 2013
- Full Text
- View/download PDF
41. Image-guided transplantation of single cells in the bone marrow of live animals
- Author
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Turcotte, Raphaël, Alt, Clemens, Runnels, Judith M., Ito, Kyoko, Wu, Juwell W., Zaher, Walid, Mortensen, Luke J., Silberstein, Lev, Côté, Daniel C., Kung, Andrew L., Ito, Keisuke, and Lin, Charles P.
- Abstract
Transplantation of a single hematopoietic stem cell is an important method for its functional characterization, but the standard transplantation protocol relies on cell homing to the bone marrow after intravenous injection. Here, we present a method to transplant single cells directly into the bone marrow of live mice. We developed an optical platform that integrates a multiphoton microscope with a laser ablation unit for microsurgery and an optical tweezer for cell micromanipulation. These tools allow image-guided single cell transplantation with high spatial control. The platform was used to deliver single hematopoietic stem cells. The engraftment of transplants was tracked over time, illustrating that the technique can be useful for studying both normal and malignant stem cells in vivo.
- Published
- 2017
- Full Text
- View/download PDF
42. Differential stem- and progenitor-cell trafficking by prostaglandin E2 .
- Author
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Hoggatt, Jonathan, Mohammad, Khalid S., Singh, Pratibha, Hoggatt, Amber F., Chitteti, Brahmananda R., Speth, Jennifer M., Hu, Peirong, Poteat, Bradley A., Stilger, Kayla N., Ferraro, Francesca, Silberstein, Lev, Wong, Frankie K., Farag, Sherif S., Czader, Magdalena, Milne, Ginger L., Breyer, Richard M., Serezani, Carlos H., Scadden, David T., Guise, Theresa A., and Srour, Edward F.
- Subjects
PROGENITOR cells ,STEM cells ,BLOOD cells ,HEMATOPOIETIC stem cells ,HOMEOSTASIS ,OSTEOPONTIN ,THERAPEUTICS - Abstract
To maintain lifelong production of blood cells, haematopoietic stem cells (HSCs) are tightly regulated by inherent programs and extrinsic regulatory signals received from their microenvironmental niche. Long-term repopulating HSCs reside in several, perhaps overlapping, niches that produce regulatory molecules and signals necessary for homeostasis and for increased output after stress or injury. Despite considerable advances in the specific cellular or molecular mechanisms governing HSC-niche interactions, little is known about the regulatory function in the intact mammalian haematopoietic niche. Recently, we and others described a positive regulatory role for prostaglandin E
2 (PGE2 ) on HSC function ex vivo. Here we show that inhibition of endogenous PGE2 by non-steroidal anti-inflammatory drug (NSAID) treatment in mice results in modest HSC egress from the bone marrow. Surprisingly, this was independent of the SDF-1-CXCR4 axis implicated in stem-cell migration. Stem and progenitor cells were found to have differing mechanisms of egress, with HSC transit to the periphery dependent on niche attenuation and reduction in the retentive molecule osteopontin. Haematopoietic grafts mobilized with NSAIDs had superior repopulating ability and long-term engraftment. Treatment of non-human primates and healthy human volunteers confirmed NSAID-mediated egress in other species. PGE2 receptor knockout mice demonstrated that progenitor expansion and stem/progenitor egress resulted from reduced E-prostanoid 4 (EP4) receptor signalling. These results not only uncover unique regulatory roles for EP4 signalling in HSC retention in the niche, but also define a rapidly translatable strategy to enhance transplantation therapeutically. [ABSTRACT FROM AUTHOR]- Published
- 2013
- Full Text
- View/download PDF
43. In vivo imaging of Treg cells providing immune privilege to the haematopoietic stem-cell niche.
- Author
-
Fujisaki, Joji, Wu, Juwell, Carlson, Alicia L., Silberstein, Lev, Putheti, Prabhakar, Larocca, Rafael, Gao, Wenda, Saito, Toshiki I., Celso, Cristina Lo, Tsuyuzaki, Hitoshi, Sato, Tatsuyuki, Côté, Daniel, Sykes, Megan, Strom, Terry B., Scadden, David T., and Lin, Charles P.
- Subjects
STEM cells ,DRUG therapy ,TESTIS ,HAIR follicles ,PLACENTA ,HOMOGRAFTS ,IMMUNOREGULATION ,BONE marrow - Abstract
Stem cells reside in a specialized regulatory microenvironment or niche, where they receive appropriate support for maintaining self-renewal and multi-lineage differentiation capacity. The niche may also protect stem cells from environmental insults including cytotoxic chemotherapy and perhaps pathogenic immunity. The testis, hair follicle and placenta are all sites of residence for stem cells and are immune-suppressive environments, called immune-privileged sites, where multiple mechanisms cooperate to prevent immune attack, even enabling prolonged survival of foreign allografts without immunosuppression. We sought to determine if somatic stem-cell niches more broadly are immune-privileged sites by examining the haematopoietic stem/progenitor cell (HSPC) niche in the bone marrow, a site where immune reactivity exists. We observed persistence of HSPCs from allogeneic donor mice (allo-HSPCs) in non-irradiated recipient mice for 30?days without immunosuppression with the same survival frequency compared to syngeneic HSPCs. These HSPCs were lost after the depletion of FoxP3 regulatory T (T
reg ) cells. High-resolution in vivo imaging over time demonstrated marked co-localization of HSPCs with Treg cells that accumulated on the endosteal surface in the calvarial and trabecular bone marrow. Treg cells seem to participate in creating a localized zone where HSPCs reside and where Treg cells are necessary for allo-HSPC persistence. In addition to processes supporting stem-cell function, the niche will provide a relative sanctuary from immune attack. [ABSTRACT FROM AUTHOR]- Published
- 2011
- Full Text
- View/download PDF
44. Enhanced Hematovascular Contribution of SCL 3′ Enhancer Expressing Fetal Liver Cells Uncovers Their Potential to Integrate in Extramedullary Adult Niches.
- Author
-
GARCIA-ORTEGA, ANTONIO M., CAÑETE, ANA, QUINTER, CRISTINA, SILBERSTEIN, LEV, PIQUER-GIL, MARINA, ALVAREZ-DOLADO, MANUEL, DEKEL, BENJAMIN, GOTTGENS, BERTHILD, and SÁNCHEZ, MARIA-JOSE
- Subjects
FETAL liver cells ,BONE marrow ,STEM cells ,LEUKEMIA ,ALKALINE phosphatase ,VASCULAR endothelium ,FLOW cytometry ,LABORATORY mice - Abstract
Fetal liver (FL) hematopoietic progenitors have superior blood engraftment competence compared with adult bone marrow (BM), however less is known about FL in vivo vascular capacity. Here we show in transplantation assays that FL cells possess enhanced vascular endothelial potential compared with adult bone marrow. We generated high-level hematopoietic chimeras using donor cells from mice transgenic for the stem cell leukaemia 3′ enhancer human placental alkaline phosphatase (SCL3′Enh-PLAP) reporter construct, active in vascular endothelium, and blood progenitor and stem cells. Long-term lineage tracing analysis revealed PLAP
+ vascular-like patches in FL-derived chimeras, whereas adult BM-derived chimeras presented only rare and scattered PLAP1 cells. PLAP+ vascular-like patches were formed following transplantation into both newborn and adult recipient mice, although their frequency was reduced in adult recipients. Confocal analysis of multiple labeled tissues revealed that whereas most liver and heart PLAP+ vascular patch-associated cells were endothelial, PLAP+ vascular patches in the kidney contained endothelial, hematopoietic, and putative hemangioblastic cells. Moreover, fluorescenceactivated cell sorting assays showed that only FL PLAPbright+ donor cells can generate PLAP+ vascular patches upon transplantation. Taken together, these data demonstrate superior vascular contribution potential of FL cells, and not only provide new insights into the developmental pathways controlling endothelial development but also may prove informative when addressing the mechanisms involved in vascular regeneration and hemangiogenic recovery in a clinical context. [ABSTRACT FROM AUTHOR]- Published
- 2010
- Full Text
- View/download PDF
45. Cell Cycle Analysis of Hematopoietic Stem and Progenitor Cells by Multicolor Flow Cytometry
- Author
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Galvin, Amy, Weglarz, Meredith, Folz‐Donahue, Kat, Handley, Maris, Baum, Misa, Mazzola, Michael, Litwa, Hannah, Scadden, David T., and Silberstein, Lev
- Abstract
Maintenance of hematopoietic stem cell (HSC) quiescence is critical for self‐renewal and differentiation into mature lineages. Therefore, the ability to reliably detect abnormal HSC cycling is essential for experiments that seek to investigate abnormalities of HSC function. The ability to reproducibly evaluate cell cycle status in a rare cell subset requires careful optimization of multiple parameters during cell preparation and sample processing. Here, we describe a method where data acquisition parameters and fluorochrome combination for long‐term HSC staining have been specifically designed for concurrent use with DAPI and Ki‐67 antibodies. © 2018 by John Wiley & Sons, Inc.
- Published
- 2019
- Full Text
- View/download PDF
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