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3 results on '"Signalisation Calcique et Immunité Végétale"'

1. Sphingolipid-induced cell death in Arabidopsis is negatively regulated by the papain-like cysteine protease RD21

Author

Kamal, Khaled, Boudsocq, Marie, Cotelle, Valérie, Mazars, Christian, Le Ru, Aurélie, Duboé, Carine, Jin, Hailing, Plaza, Serge, Combier, Jean-Philippe, Ormancey, Mélanie, Thuleau, Patrice, van der Hoorn, Renier A.L., Grat, Sabine, Testard, Ambroise, Plateforme Imagerie Microscopie, Fédération de Recherche FR3450, Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Centre de biologie du développement (CBD), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre de Biologie Intégrative (CBI), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Department of Plant Pathology & Microbiology [Riverside], University of California [Riverside] (UCR), University of California-University of California, Laboratoire de Recherche en Sciences Végétales (LRSV), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), The Plant Chemetics Laboratory, Department of Plant Sciences, University of Oxford [Oxford], Peptides et petits ARN, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Signalisation Calcique et Immunité Végétale, Agronomy Department, Faculty of Agriculture, Zagazig University, Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Signalisation Cellulaire et Ubiquitination, Centre National de la Recherche Scientifique, University de Toulouse, French Laboratory of Excellence project 'TULIP' [ANR-10-LABX-41, ANR-11-IDEX-0002-02], ERC Consolidator grant [616449], French Laboratory of Excellence project 'SPS' [ANR-10-LABX-0040-SPS], IFE-STDF [4/2016-2017], Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), University of Oxford, Zagazig University, ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010), ANR-11-IDEX-0002,UNITI,Université Fédérale de Toulouse(2011), ANR-10-LABX-0040,SPS,Saclay Plant Sciences(2010), European Project: 616449,EC:FP7:ERC,ERC-2013-CoG,GREENPROTEASES(2014), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11), and Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
0106 biological sciences, 0301 basic medicine, Programmed cell death, Proteases, papain-like cysteine proteases, [SDV]Life Sciences [q-bio], Arabidopsis, Plant Science, 01 natural sciences, 03 medical and health sciences, chemistry.chemical_compound, Gene Expression Regulation, Plant, Papain, Genetics, Arabidopsis thaliana, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Ceramide synthase, programmed cell death, ComputingMilieux_MISCELLANEOUS, activity-based protein profiling, Fumonisin B1, Sphingolipids, biology, Cell Death, Arabidopsis Proteins, food and beverages, General Medicine, biology.organism_classification, Cysteine protease, Sphingolipid, Cell biology, 030104 developmental biology, chemistry, fumonisin b1, Agronomy and Crop Science, 010606 plant biology & botany, Signal Transduction
Abstract

International audience; It is now well established that sphingoid Long Chain Bases (LCBs) are crucial mediators of programmed cell death. In plants, the mycotoxin fumonisin B1 (FM) produced by the necrotrophic fungus Fusarium moniliforme disrupts the sphingolipid biosynthesis pathway by inhibiting the ceramide synthase leading to an increase in the amount of phytosphingosine (PHS) and dihydrosphingosine (DHS), the two major LCBs in Arabidopsis thaliana. To date, the signaling pathway involved in FB1-induced cell death remains largely uncharacterized. It is also well acknowledged that plant proteases such as papain-like cysteine protease are largely involved in plant immunity. Here, we show that the papain-like cysteine protease RD21 (responsive-to-desiccation-21) is activated in response to PHS and FB1 in Arabidopsis cultured cells and leaves, respectively. Using two allelic null mutants of RD21, and two different PCD bioassays, we demonstrate that the protein acts as a negative regulator of FB1-induced cell death in Arabidopsis.

Published
2019
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2. Microgravity induces changes in microsome-associated proteins of Arabidopsis seedlings grown on board the international space station

Author

Annick Graziana, Sabine Grat, Elodie Boucheron-Dubuisson, Brigitte Eche, Eugénie Carnero-Diaz, Francisco Javier Medina, Isabel Le Disquet, Christian Brière, Michel Rossignol, Carole Pichereaux, Veronica Pereda-Loth, Christian Mazars, Laboratoire de Recherche en Sciences Végétales (LRSV), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Signalisation Calcique et Immunité Végétale, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Institut de pharmacologie et de biologie structurale (IPBS), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Groupement scientifique de Biologie et de Medecine Spatiale (GSBMS), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National d'Études Spatiales [Toulouse] (CNES), Physiologie cellulaire et moléculaire des plantes (PCMP), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Centro de Investigaciones Biológicas (CSIC), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), and Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National d'Études Spatiales [Toulouse] (CNES)

Subjects
0106 biological sciences, Proteomics, Science, Plant Cell Biology, Arabidopsis, Plant Science, 01 natural sciences, Biochemistry, 03 medical and health sciences, Auxin, Plant-Environment Interactions, Microsomes, Botany, Biology, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Multidisciplinary, biology, Weightlessness, Extraterrestrial Life, Plant Biochemistry, Arabidopsis Proteins, Plant Ecology, Proteins, Membrane Proteins, Space Flight, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, biology.organism_classification, Astrobiology, Transport protein, Cell biology, Protein Transport, Phenotype, Membrane protein, chemistry, Seedling, Seedlings, Plant Physiology, Proteome, Medicine, Protein Abundance, 010606 plant biology & botany, Research Article
Abstract

18 p.-8 fig.-2 tab. Mazars, Christian et alt., The ‘‘GENARA A’’ experiment was designed to monitor global changes in the proteome of membranes of Arabidopsis thaliana seedlings subjected to microgravity on board the International Space Station (ISS). For this purpose, 12-day-old seedlings were grown either in space, in the European Modular Cultivation System (EMCS) under microgravity or on a 1 g centrifuge, or on the ground. Proteins associated to membranes were selectively extracted from microsomes and identified and quantified through LC-MS-MS using a label-free method. Among the 1484 proteins identified and quantified in the 3 conditions mentioned above, 80 membrane-associated proteins were significantly more abundant in seedlings grown under microgravity in space than under 1 g (space and ground) and 69 were less abundant. Clustering of these proteins according to their predicted function indicates that proteins associated to auxin metabolism and trafficking were depleted in the microsomal fraction in mg space conditions, whereas proteins associated to stress responses, defence and metabolism were more abundant in mg than in 1 g indicating that microgravity is perceived by plants as a stressful environment. These results clearly indicate that a global membrane proteomics approach gives a snapshot of the cell status and its signaling activity in response to microgravity and highlight the major processes affected., Funding was supported by CNRS -CNES - Université Paul Sabatier.

Published
2014
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3. Microsome-associated proteome modifications ofArabidopsisseedlings grown on board the International Space Station reveal the possible effect on plants of space stresses other than microgravity

Author

Carole Pichereaux, Annick Graziana, Eugénie Carnero-Diaz, Brigitte Eche, Veronica Pereda-Loth, Michel Rossignol, Sabine Grat, Francisco Javier Medina, Christian Brière, Christian Mazars, Elodie Boucheron-Dubuisson, Isabel Le Disquet, European Space Agency, Centre National D'Etudes Spatiales (France), Mazars, Christian, Pichereaux, Carole, Pereda-Loth, Veronica, Boucheron-Dubuisson, Élodie, Medina, F. Javier, Carnero-Díaz, Eugénie, Laboratoire de Recherche en Sciences Végétales (LRSV), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Signalisation Calcique et Immunité Végétale, Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Plateforme protéomique, Institut de pharmacologie et de biologie structurale (IPBS), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Groupement scientifique de Biologie et de Medecine Spatiale (GSBMS), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National d'Études Spatiales [Toulouse] (CNES), Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Centro de Investigaciones Biológicas (CSIC), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre National d'Études Spatiales [Toulouse] (CNES)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Muséum national d'Histoire naturelle (MNHN)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Mazars, Christian [0000-0002-0670-6042], Pichereaux, Carole [0000-0002-7559-5358], Pereda-Loth, Veronica [0000-0002-7365-6217], Boucheron-Dubuisson, Élodie [0000-0001-9914-1740], Medina, F. Javier [0000-0002-0866-7710], and Carnero-Díaz, Eugénie [0000-0002-3771-3106]

Subjects
quantitative proteomics, Extraterrestrial Environment, Proteome, Arabidopsis thaliana, Short Communication, Gravity, Arabidopsis, Aquaporin, membrane proteins, Plant Science, Biology, International Space Station, label-free, spaceflight, Stress, Physiological, Ribosomal protein, Microsomes, Membrane proteins, Botany, Quantitative proteomics, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Life support system, Arabidopsis Proteins, Weightlessness, Spaceflight, Space Flight, biology.organism_classification, gravity, Cell biology, Membrane protein, Seedlings, Label-free
Abstract

11p.-2 fig.-6 tab., Growing plants in space for using them in bioregenerative life support systems during long-term human spaceflights needs improvement of our knowledge in how plants can adapt to space growth conditions. In a previous study performed on board the International Space Station (GENARA A experiment STS-132) we evaluate the global changes that microgravity can exert on the membrane proteome of Arabidopsis seedlings. Here we report additional data from this space experiment, taking advantage of the availability in the EMCS of a centrifuge to evaluate the effects of cues other than microgravity on the relative distribution of membrane proteins. Among the 1484 membrane proteins quantified, 227 proteins displayed no abundance differences between µ g and 1 g in space, while their abundances significantly differed between 1 g in space and 1 g on ground. A majority of these proteins (176) were over-represented in space samples and mainly belong to families corresponding to protein synthesis, degradation, transport, lipid metabolism, or ribosomal proteins. In the remaining set of 51 proteins that were under-represented in membranes, aquaporins and chloroplastic proteins are majority. These sets of proteins clearly appear as indicators of plant physiological processes affected in space by stressful factors others than microgravity., The authors would like to thank the National Aeronautics and Space Administration (NASA) who successfully performed the spaceflight experiment; they also thank the astronauts for performing the required tasks on board the ISS. We acknowledge the Norwegian User Support and Operations Center team (NUSOC) for the ground and space preparation of the GENARA-A experiment and we thank the European Aeronautic Defense and Space Company (Astrium EADS) for the design and building of the hardware. We also thank the European Space Agency (ESA) and the Centre National d’Etudes Spatiales(CNES) for their scientific and financial support.

Published
2014
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