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Investigates some plasma coagulation parameters particularly with respect to F.XIII subunits of ulcerative colitis (UC) patients in relation to the active and quiescent UC stages. Materials and methods; Coagulation findings in UC active patients; Overview of hemostatic parameters in UC patients at remission.

This paper presents an attempt to assess the potential use of Calamagrostis epigejos (L.) Roth. as a renewable energy source. Abandonment of human management is often followed by a decrease in species richness in semi-natural grasslands, mainly due to the increased dominance of clonal grasses such as Calamagrostis epigejos which were formerly repressed by management. The biomass resource of this, and its accompanying, species, i.e. species of the Solidago genus and others e.g. Cirsium rivulare, Deschampsia caespitosa, Molinia coerulea and Filipendula ulmaria, was evaluated in the green wastelands of the River Bytomka valley (Upper Silesia, Poland). It was found that approx. 1.2 t·ha−1 of dry matter can be obtained from approx. 30% of the average share of Calamagrostis epigejos in plant communities of unmown meadows. This is 10 times less than in the case of Miscanthus giganteus, a non-native cultivated grass. An increase in the biomass component of Calamagrostis epigejos reduced that of Solidago sp. (−0.522176, p< 0.05) and other species (−0.465806, p< 0.05). The calorific value of Calamagrostis epigejos biomass is approx. 15.91 MJ·kg−1, which is comparable to the calorific value of coal and close to, inter alia, that of Miscanthus sacchariflorus (19 MJ·kg−1) as an energy crop. The presented research is in its preliminary stages and therefore, it is necessary to investigate the reaction of Calamagrostis epigejos to regular mowing and to removal of the biomass from the studied areas.

The Water Framework Directive introduced an ecological approach to the assessment and classification of waters. The part of the assessmentare macrophyte - based methods used in the evaluation of the ecologicalcondition of rivers in respect of biodiversity. The Macrophyte Method for River Assessment has been used in Poland since 2007 and provides us with an information about ecological state of the rivers. Presented analyses were aimed to describe macrophyte development in the sandylowland type of river in the Silesian Upland. The assessment was made for lowland Pszczynka River (45.8 km total length, total catchment area 368.3 km2), which is a left-bank tributary of Vistula River. At the selected 10 research sections of the river (100 m each) the composition of macrophytes and abiotic conditions of the river were described. The paper presents the results of preliminary assessment of ecological condition of Pszczynka River on the background sources of substances discharged to the riveralongits course. Macrophyte Index for Rivers (MIR) was calculated based on the results of the research - river bed surface coverage by the particular plant species. The MIR limit values were used to determine the class of water quality in the examined river. Overall, the ecological status of Pszczynka River was rated as moderate and its water classified as Class III of water quality.

Studies of in situ plant response and adaptation to complex environmental stresses, are crucial for understanding the mechanisms of formation and functioning of ecosystems of anthropogenically transformed habitats. We study short- and long-term responses of photosynthetic apparatus (PSA) and anti-oxidant capacity to complex abiotic stresses of common plants Calamagrostis epigejos and Solidago gigantea in semi-natural (C) and heavy metal contaminated habitats (LZ). We found significant differences in leaf pigment content between both plant species growing on LZ plots and their respective C populations. The average values of leaf chlorophyll indexes were 27% lower in the LZ populations of both species and significantly lower in Sg plants in comparison to Ce ones. The average values of the anthocyanin index in CeLZ and SgLZ populations were significantly higher (by 18%) than in their respective controls. In both Ce and Sg plants occurring on LZ plots, the average leaf flavonol indexes were higher than on their controls by 31% and 15% and this index was significantly higher in SgLZ population than CeLZ and CeC plants (by 34% and 54%, respectively). Both Ce and Sg populations growing on LZ plots showed significantly lower photosynthetic rate (A), transpiration rate (E) and stomatal conductance (g s ) in comparison to controls. On the other hand, a significantly higher photosynthetic rate was detected in SgLZ than in CeLZ populations. The catalase activities were significantly higher than recorded in Sg than in Ce tissues, irrespective of the plot type. They were also higher in LZ populations than those in controls for both species. Moreover, the H 2 O 2 content in Sg tissues was significantly higher than those in Ce. Hydrogen peroxide content in CeLZ and SgLZ were respectively 39% (non-significant) and 57% higher, compared to their controls. The reverse pattern was found in the case of MDA, whose concentration was significantly higher in the leaves of Ce population compared to the control population. The average MDA concentration in CeLZ populations was 17% higher than in the CeC. In the case of Sg no significant differences were found. Mechanisms of plant species adaptation to industrial areas are crucial for species selection and planning effective reclamation of them. The analysis of chlorophyll fluorescence induction curves as well as well as the results of JIP test revealed the decreased of Fj value despite positive ΔK-band in SgLZ and CeLZ plants suggesting the increased rate of electron transfer from Q A to Q B at the acceptor side of PSII, thus a high quantity of P 680+ and/or effective quenching by exogenous molecules. The increase in the I-P part of the induction curve typically attributed to the reduction of electron transporters (ferredoxin, intermediary acceptors, and NADP) of the PSI acceptor side was observed in both SgC and SgLZ but not in CeLZ populations. These changes demonstrate species-specific effects on electron transport during the light phase of photosynthesis under complex environmental stress. Our results show that Sg and Ce individuals developed a range of structural and functional adaptations to protect PSA against complex environmental stresses (possible combination of heavy metals, water deficiency, temperature, nutrient deficiency and salinity). Both species from LZ plots could tolerate high levels of Cd, Zn and Pb in leaf tissues. Therefore they can be potential candidates for use in phytoremediation of HM contaminated areas. However, further long-term field and experimental research on plant traits response and adaptation to complex environmental stresses on industrial habitats are needed., Competing Interests: Declarations. Competing interests: The authors declare no competing interests., (© 2024. The Author(s).)

Adenosine is one of the building blocks of nucleic acids and other biologically important molecules. Spectroscopic methods have been among the most utilized techniques to study adenosine and its derivatives. However, most of them deal with adenosine in solution. Here, we present the first vibrational circular dichroism (VCD) spectroscopic study of adenosine crystals in solid state. Highly regular arrangement of adenosine molecules in a crystal resulted in a strongly enhanced supramolecular VCD signal originating from long-range coupling of vibrations. The data suggested that adenosine crystals, in contrast to guanosine ones, do not imbibe atmospheric water. Relatively large dimensions of the adenosine crystals resulted in scattering and substantial orientational artifacts affecting the spectra. Several strategies for tackling the artifacts have been proposed and tested. Atypical features in IR absorption spectra of crystalline adenosine (e.g., extremely low absorption in mid-IR spectral range) were observed and attributed to refractive properties of adenosine crystals., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Various plant functional groups (PFGs) used in the reclamation of post-mining heaps may differ in their nutrient uptake efficiency and thus in their effect on the ecosystem development. The effect of PFGs may be additionally modified by the applied reclamation measures such as e.g. topsoiling. In this study we compared the nutrient uptake efficiencies and plant stoichiometry for two PFGs (grasses and forbs) growing on the sites reclaimed by applying topsoil (TS) and unreclaimed sites on carboniferous bare rock (BR) in hard coal spoil heap in Upper Silesia (southern Poland). Basic soil parameters, including pH, texture, soil organic carbon, and nutrients (N, P, K, Ca, and Mg), were measured, and the aboveground plant biomass and nutrient content in plant tissue were determined. Forbs were characterized by a larger biomass and higher nutrient concentrations (except for P) than grasses. The TS treatment supported higher concentrations of N and P in plant tissues but not to the level ensuring more significant primary biomass production. The nutrient concentration and elemental stoichiometry in plant tissue indicated that N was the primary limiting element. However, the major growth limitation for N-fixing forbs was from P. Forbs were much more efficient in nutrient uptake than grasses, independent of the reclamation treatment. Therefore, they stimulate nutrient cycling in the restored ecosystems more than grasses., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Perennial ryegrass is a grass species used to establish lawns in urban areas where pollution is a major environmental problems. Cadmium (Cd) and nickel (Ni) contribute significantly to these pollutants and may cause photosynthetic limitation. The main objective of this work was to perform a comprehensive analysis of photosynthetic efficiency of perennial ryegrass seedlings under Cd and Ni stress. Some of the main indices of photosynthetic efficiency (prompt and delayed chlorophyll-a fluorescence signals and modulated reflectance at 820 nm) were compared with growth parameters. Two cultivars were tested: 'Niga' and 'Nira'. A decrease in photosystem (PS) II and PSI activity was observed. This was due to an increase in nonradiative dissipation of the PSII antenna, a decrease in PSII antenna size, or a decrease in the number of photosynthetic complexes with fully closed PSII RCs. Efficiency of electron transport was decreased. The effect on the modulated reflectance signal could indicate a restriction in electron flow from PSII to PSI. The correlation between photosynthetic efficiency parameters, such as Area, Fo, Fm, and Fv, and growth parameters, confirmed that some photosynthetic efficiency parameters can be used as indicators for early detection of heavy metal effects., (© 2023. The Author(s).)

Pesticide use cannot be completely abandoned in modern agriculture. Among agrochemicals, glyphosate is one of the most popular and, at the same time, most divisive herbicide. Since the chemicalization of agriculture is detrimental, various attempts are being made to reduce it. Adjuvants-substances that increase the efficiency of foliar application-can be used to reduce the amount of herbicides used. We propose low-molecular-weight dioxolanes as adjuvants for herbicides. These compounds quickly convert to carbon dioxide and water and do not harm plants. The aim of this study was to evaluate the efficacy of RoundUp ® 360 Plus supported by three potential adjuvants: 2,2-dimethyl-1,3-dioxolane (DMD), 2,2,4-trimethyl-1,3-dioxolane (TMD), and (2,2-dimethyl-1,3-dioxan-4-yl)methanol (DDM), on a common weed species Chenopodium album L., under greenhouse conditions. Chlorophyll a fluorescence parameters and analysis of the polyphasic fluorescence (OJIP) curve, which examines changes in the photochemical efficiency of photosystem II, were used to measure plant sensitivity to glyphosate stress and verified the efficacy achieved by tested formulations. The effective dose (ED) values obtained showed that the weed tested was sensitive to reduced doses of glyphosate, with 720 mg/L needed to achieve 100% effectiveness. Compared to the glyphosate assisted with DMD, TMD, and DDM, ED was reduced by 40%, 50%, and 40%, respectively. The application of all dioxolanes at a concentration equal to 1 vol.% significantly enhanced the herbicide's effect. Our study showed that for C. album there was a correlation between the change in OJIP curve kinetics and the applied dose of glyphosate. By analyzing the discrepancies in the curves, it is possible to show the effect of different herbicide formulations with or without dioxolanes at an early stage of its action, thus minimizing the time for testing new substances as adjuvants.

Invited for the cover of this issue are Valery Andrushchenko, Monika Krupová, and co-workers at the Institute of Organic Chemistry and Biochemistry (IOCB Prague) of the Czech Academy of Sciences. The image depicts a "crystal city" illuminated by "chiral suns" shining left- and right-circularly polarized light (L-CPL and R-CPL), which reveals differences in the structure of the chiral crystalline "skyscrapers". Designed by Tomáš Belloň @ IOCB Prague. Read the full text of the article at 10.1002/chem.202201922., (© 2022 Wiley-VCH GmbH.)

Due to advances in the industrial development of light-emitting diodes (LEDs), much research has been conducted in recent years to get a better understanding of how plants respond to these light sources. This study investigated the effects of different LED-based light regimes on strawberry plant development and performance. The photosynthetic pigment content, biochemical constituents, and growth characteristics of strawberry plants were investigated using a combination of different light intensities (150, 200, and 250 μmol m -2 s -1 ), qualities (red, green, and blue LEDs), and photoperiods (14/10 h, 16/8 h, and 12/12 h light/dark cycles) compared to the same treatment with white fluorescent light. Plant height, root length, shoot fresh and dry weight, chlorophyll a , total chlorophyll/carotenoid content, and most plant yield parameters were highest when illuminated with LM7 [intensity (250 μmol m -2 s -1 ) + quality (70% red/30% blue LED light combination) + photoperiod (16/8 h light/dark cycles)]. The best results for the effective quantum yield of PSII photochemistry Y(II), photochemical quenching coefficient (qP), and electron transport ratio (ETR) were obtained with LM8 illumination [intensity (250 μmol m -2 s -1 ) + quality (50% red/20% green/30% blue LED light combination) + photoperiod (12 h/12 h light/dark cycles)]. We conclude that strawberry plants require prolonged and high light intensities with a high red-light component for maximum performance and biomass production., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Guiamba, Zhang, Sierka, Lin, Ali, Ali, Lamlom, Kalaji, Telesiński, Yousef and Xu.)

Identifying environmental factors that improve plant growth and development under nitrogen (N) constraint is essential for sustainable greenhouse production. In the present study, the role of light intensity and N concentrations on the biomass partitioning and physiology of chrysanthemum was investigated. Four light intensities [75, 150, 300, and 600 µmol m -2  s -1 photosynthetic photon flux density (PPFD)] and three N concentrations (5, 10, and 15 mM N L -1 ) were used. Vegetative and generative growth traits were improved by increase in PPFD and N concentration. High N supply reduced stomatal size and g s in plants under lowest PPFD. Under low PPFD, the share of biomass allocated to leaves and stem was higher than that of flower and roots while in plants grown under high PPFD, the share of biomass allocated to flower and root outweighed that of allocated to leaves and stem. As well, positive effects of high PPFD on chlorophyll content, photosynthesis, water use efficiency (WUE), Nitrogen use efficiency (NUE) were observed in N-deficient plants. Furthermore, photosynthetic functionality improved by raise in PPFD. In conclusion, high PPFD reduced the adverse effects of N deficiency by improving photosynthesis and stomatal functionality, NUE, WUE, and directing biomass partitioning toward the floral organs., (© 2022. The Author(s).)

Copper (Cu) is an essential element involved in various metabolic processes in plants, but at concentrations above the threshold level, it becomes a potential stress factor. The effects of two different cytokinins, kinetin (KIN) and 6-benzylaminopurine (BAP), on chlorophyll a fluorescence parameters, stomatal responses and antioxidation mechanisms in castor ( Ricinus communis L.) under Cu 2+ toxicity was investigated. Ricinus communis plants were exposed to 80 and 160 μM CuSO 4 added to the growth medium. Foliar spraying of 15 μM KIN and BAP was carried out on these seedlings. The application of these cytokinins enhanced the tissue water status, chlorophyll contents, stomatal opening and photosynthetic efficiency in the castor plants subjected to Cu 2+ stress. The fluorescence parameters, such as Fm, Fv/Fo, Sm, photochemical and non-photochemical quantum yields, energy absorbed, energy trapped and electron transport per cross-sections, were more efficiently modulated by BAP application than KIN under Cu 2+ toxicity. There was also effective alleviation of reactive oxygen species by enzymatic and non-enzymatic antioxidation systems, reducing the membrane lipid peroxidation, which brought about a relative enhancement in the membrane stability index. Of the various treatments, 80 µM CuSO 4 + BAP recorded the highest increase in photosynthetic efficiency compared to other cytokinin treatments. Therefore, it can be concluded that BAP could effectively alleviate the detrimental effects of Cu 2+ toxicity in cotyledonary leaves of R. communis by effectively modulating stomatal responses and antioxidation mechanisms, thereby enhancing the photosynthetic apparatus' functioning.

Satureja khuzistanica Jamzad is a valuable and endemic medicinal plant. Boron and zinc are essential elements for the vegetative and reproductive growth of plants and have significant effects on yield, essential oil composition and the seed production of plants. To investigate the effects of the foliar application of zinc and boron on the growth, yield, seed production and phytochemical properties of S. khuzistanica , a study was conducted in a factorial experiment with three replicates in two consecutive years based on a randomized complete block design. The foliar application of boron (B) at three concentrations (control or distilled water, 0.4% and 0.8% as H 3 BO 3 ) and zinc (Zn) at three concentrations (control or distilled water, 0.3% and 0.6% as ZnSO 4 ) was carried out. Our results showed that the foliar application of B resulted in a significant increase in the fresh and dry weights of plants, the dry weight of stems, drug yield, seed yield, seed germination and 1000-seed weight. At the same time, the application of B resulted in a significant decrease in seed emptiness. The fresh and dry weights of plants, drug yield, seed yield, 1000-seed weight and seed germination were also significantly improved by Zn foliar spraying compared to the control. Application of 0.8% B resulted in a significant decrease in seed emptiness by 14.16% and 22.37%, as compared to the control. The foliar spraying of B and Zn improved the total phenolic content, the essential oil content and the yield and antioxidant activity of S. khuzistanica . Moreover, B application generally concentrated more carvacrol in the essential oil (in the first experimental year). In contrast, no significant differences were observed between Zn treatments in carvacrol content and total flavonoids. The use of several microelements, such as B and Zn, could improve both the quantity and quality of S. khuzistanica . Additionally, improvement of seed set and seed quality by the foliar spraying of Zn and B may be useful for growing plants in arid and semi-arid areas.

GABA (gamma-aminobutyric acid) and melatonin are endogenous compounds that enhance plant responses to abiotic stresses. The response of Vicia faba to different stressors (salinity (NaCl), poly ethylene glycol (PEG), and sulfur dioxide (SO 2 )) was studied after priming with sole application of GABA and melatonin or their co-application (GABA + melatonin). Both melatonin and GABA and their co-application increased leaf area, number of flowers, shoot dry and fresh weight, and total biomass. Plants treated with GABA, melatonin, and GABA + melatonin developed larger stomata with wider aperture compared to the stomata of control plants. The functionality of the photosynthetic system was improved in primed plants. To investigate the photosynthetic functionality in details, the leaf samples of primed plants were exposed to different stressors, including SO 2 , PEG, and NaCl. The maximum quantum yield of photosystem II (PS II) was higher in the leaf samples of primed plants, while the non-photochemical quenching (NPQ) of primed plants was decreased when leaf samples were exposed to the stressors. Correlation analysis showed the association of initial PI abs with post-stress F V /F M and NPQ. Stressors attenuated the association of initial PI abs with both F V /F M and NPQ, while priming plants with GABA, melatonin, or GABA + melatonin minimized the effect of stressors by attenuating these correlations. In conclusion, priming plants with both GABA and melatonin improved growth and photosynthetic performance of Vicia faba and mitigated the effects of abiotic stressors on the photosynthetic performance.

Knowledge about biotic (plant species diversity, biomass) and/or abiotic (physicochemical substrate parameters) factors that determine enzyme activity and functional diversity of the substrate on hard coal spoil heaps is limited. Spontaneously developed vegetation patches dominated by herbaceous species commonly occurring on these spoil heaps: grasses (Poa compressa, Calamagrostis epigejos) and forbs (Daucus carota, Tussilago farfara), were examined. The activity of dehydrogenase and alkaline phosphatase was twice as high in plots dominated by grass species compared with those dominated by forbs. Significant positive correlations were found between the activity of dehydrogenase and alkaline phosphatase with pH, available P, soil moisture, and water holding capacity and negative correlations between the activity of urease and soil organic carbon. Strong positive correlations were found between values for Shannon-Wiener diversity index, evenness, species richness and soil functional diversity in plots dominated by grasses. We found that the soil physicochemical parameters had a greater impact on enzyme activity of the substrate than plant biomass and species diversity. However, grasses, through their extensive root system, more effectively increased enzyme activity and health of the substrate than other herbaceous species, and as they stabilize the substrate and form dense plant cover, they can be recommended for reclamation purposes.

Excess circulating fatty acids contribute to endothelial dysfunction that subsequently aggravates the metabolic conditions such as fatty liver diseases. However, the exact mechanism of this event is not fully understood, and the investigation on the effect of a direct exposure to fatty acids together with their subsequent fate is of interest. In this work we employed a chemically specific and label-free techniques such as Raman and CARS microscopies, to investigate the process of lipid droplets (LDs) formation in endothelial cells and hepatocytes after exposure to oleic and palmitic acid. We aimed to observe the changes in the composition of LDs associated with metabolism and degradation of lipids. We were able to characterize the diversity in the formation of LDs in endothelium as compared to hepatocytes, as well as the differences in the formation of LDs and degradation manner with respect to the used fatty acid. Thus, for the first time the spectral characteristics of LDs formed in endothelial cells after incubation with oleic and palmitic acid is presented, including the time-dependent changes in their chemical composition., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Liver sinusoidal endothelial cells (LSECs), a type of endothelial cells with unique morphology and function, play an important role in the liver hemostasis, and LSECs dysfunction is involved in the development of nonalcoholic fatty liver disease (NAFLD). Here, we employed Raman imaging and chemometric data analysis in order to characterize the presence of lipid droplets (LDs) and their lipid content in primary murine LSECs, in comparison with hepatocytes, isolated from mice on high-fat diet. On NAFLD development, LDs content in LSECs changed toward more unsaturated lipids, and this response was associated with an increased expression of stearylo-CoA desaturase-1. To the best of our knowledge, this is a first report characterizing LDs in LSECs, where their chemical composition is analyzed along the progression of NAFLD at the level of single LD using Raman imaging., (© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Vascular plants are able to conduct biomineralization processes and collect synthesized compounds in their internal tissues or to deposit them on their epidermal surfaces. This mechanism protects the plant from fluctuations of nutrient levels caused by different levels of supply and demand for them. The biominerals reflect both the metabolic characteristics of a vascular plant species and the environmental conditions of the plant habitat. The SEM/EDX method was used to examine the surface and cross-sections of the Calamagrostis epigejos and Phragmites australis leaves from post-industrial habitats (coal and zinc spoil heaps). The results from this study have showed the presence of mineral objects on the surfaces of leaves of both grass species. The calcium oxalate crystals, amorphous calcium carbonate spheres, and different silica forms were also found in the inner tissues. The high variety of mineral forms in the individual plants of both species was shown. The waxes observed on the leaves of the studied plants might be the initializing factor for the crystalline forms and structures that are present. For the first time, wide range of crystal forms is presented for C. epigejos. The leaf samples of P. australis from the post-industrial areas showed an increased amount of mineral forms with the presence of sulfur.

Background/aim: Reactive oxygen species play an important role in the pathogenesis of acute pancreatitis (AP) in animal models. Data on the oxidant-antioxidant balance in humans are scanty. The present study was undertaken to evaluate the dynamics of changes in the oxidant-antioxidant balance in the early phase of human AP., Methods: 74 consecutive patients with acute biliary pancreatitis (16 with severe, 58 with mild pancreatitis), treated endoscopically, were included in the study. Serum concentrations of sulfhydryl groups (SH; main nonenzymatic antioxidant; 73 patients) and thiobarbituric acid reactive substances (TBARS; markers of reactive oxygen species-mediated tissue damage; 56 patients) were determined on admission and on each of 10 successive days. The analysis comprised the comparison of results in patients with mild and severe outcome of pancreatitis., Results: Serum SH dropped by 27%, reaching the trough level on day 4 of hospitalization, whereas serum TBARS rose by 28%, reaching a peak 1 day later. Neither SH nor TBARS returned to initial values at the end of observation. The most dynamic changes in both SH and TBARS concentrations occurred in the first 3 days of hospitalization. The changes were significantly greater in patients with complicated pancreatitis in comparison to patients with mild disease, and were most pronounced in patients who developed infected pancreatic necrosis and who subsequently died., Conclusions: The oxidant-antioxidant balance changes rapidly in the early phase of human AP, confirming the role of oxidative stress in the pathogenesis of AP. The degree of changes correlates with the clinical severity of pancreatitis., (Copyright 2004 S. Karger AG, Basel and IAP)