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Your search keyword '"Sier, Vincent Q."' showing total 15 results
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15 results on '"Sier, Vincent Q."'

1. Preclinical evaluation of EpCAM-binding designed ankyrin repeat proteins (DARPins) as targeting moieties for bimodal near-infrared fluorescence and photoacoustic imaging of cancer

Author

Houvast, Ruben D., Badr, Nada, March, Taryn, de Muynck, Lysanne D. A. N., Sier, Vincent Q., Schomann, Timo, Bhairosingh, Shadhvi, Baart, Victor M., Peeters, Judith A. H. M., van Westen, Gerard J. P., Plückthun, Andreas, Burggraaf, Jacobus, Kuppen, Peter J. K., Vahrmeijer, Alexander L., and Sier, Cornelis F. M.

Published
2024
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4. Preclinical evaluation of EpCAM-binding designed ankyrin repeat proteins (DARPins) as targeting moieties for bimodal near-infrared fluorescence and photoacoustic imaging of cancer

Author

Houvast, Ruben D, Badr, Nada, March, Taryn, de Muynck, Lysanne D A N, Sier, Vincent Q, Schomann, Timo, Bhairosingh, Shadhvi, Baart, Victor M, Peeters, Judith A H M, van Westen, Gerard J P, Plückthun, Andreas; https://orcid.org/0000-0003-4191-5306, Burggraaf, Jacobus, Kuppen, Peter J K, Vahrmeijer, Alexander L, Sier, Cornelis F M, Houvast, Ruben D, Badr, Nada, March, Taryn, de Muynck, Lysanne D A N, Sier, Vincent Q, Schomann, Timo, Bhairosingh, Shadhvi, Baart, Victor M, Peeters, Judith A H M, van Westen, Gerard J P, Plückthun, Andreas; https://orcid.org/0000-0003-4191-5306, Burggraaf, Jacobus, Kuppen, Peter J K, Vahrmeijer, Alexander L, and Sier, Cornelis F M

Abstract

PURPOSE Fluorescence-guided surgery (FGS) can play a key role in improving radical resection rates by assisting surgeons to gain adequate visualization of malignant tissue intraoperatively. Designed ankyrin repeat proteins (DARPins) possess optimal pharmacokinetic and other properties for in vivo imaging. This study aims to evaluate the preclinical potential of epithelial cell adhesion molecule (EpCAM)-binding DARPins as targeting moieties for near-infrared fluorescence (NIRF) and photoacoustic (PA) imaging of cancer. METHODS EpCAM-binding DARPins Ac2, Ec4.1, and non-binding control DARPin Off7 were conjugated to IRDye 800CW and their binding efficacy was evaluated on EpCAM-positive HT-29 and EpCAM-negative COLO-320 human colon cancer cell lines. Thereafter, NIRF and PA imaging of all three conjugates were performed in HT-29_luc2 tumor-bearing mice. At 24 h post-injection, tumors and organs were resected and tracer biodistributions were analyzed. RESULTS Ac2-800CW and Ec4.1-800CW specifically bound to HT-29 cells, but not to COLO-320 cells. Next, 6 nmol and 24 h were established as the optimal in vivo dose and imaging time point for both DARPin tracers. At 24 h post-injection, mean tumor-to-background ratios of 2.60 ± 0.3 and 3.1 ± 0.3 were observed for Ac2-800CW and Ec4.1-800CW, respectively, allowing clear tumor delineation using the clinical Artemis NIRF imager. Biodistribution analyses in non-neoplastic tissue solely showed high fluorescence signal in the liver and kidney, which reflects the clearance of the DARPin tracers. CONCLUSION Our encouraging results show that EpCAM-binding DARPins are a promising class of targeting moieties for pan-carcinoma targeting, providing clear tumor delineation at 24 h post-injection. The work described provides the preclinical foundation for DARPin-based bimodal NIRF/PA imaging of cancer.

Published
2024

6. Preclinical evaluation of EpCAM-binding designed ankyrin repeat proteins (DARPins) as targeting moieties for bimodal near-infrared fluorescence and photoacoustic imaging of cancer

Author

Houvast, Ruben D., primary, Badr, Nada, additional, March, Taryn, additional, de Muynck, Lysanne D. A. N., additional, Sier, Vincent Q., additional, Schomann, Timo, additional, Bhairosingh, Shadhvi, additional, Baart, Victor M., additional, Peeters, Judith A. H. M., additional, van Westen, Gerard J. P., additional, Plückthun, Andreas, additional, Burggraaf, Jacobus, additional, Kuppen, Peter J. K., additional, Vahrmeijer, Alexander L., additional, and Sier, Cornelis F. M., additional

Published
2023
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7. Future surgeon: bridging the intergenerational gap

Author

Huizing, Floortje, primary, Sier, Vincent Q, additional, Tresfon, Jaco A S, additional, van der Vorst, Joost R, additional, Liem, Ronald S L, additional, Schmitz, Roderick F, additional, Schepers, Abbey, additional, Houwert, R Marijn, additional, Vriens, Menno R, additional, Snijders, Heleen S, additional, and Blok, Joris J, additional

Published
2023
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8. Future surgeon: bridging the intergenerational gap

Author

Zorgeenheid Traumatologie, Other research (not in main researchprogram), Heelkunde Opleiding, MS CGO, Cancer, Huizing, Floortje, Sier, Vincent Q, Tresfon, Jaco A S, van der Vorst, Joost R, Liem, Ronald S L, Schmitz, Roderick F, Schepers, Abbey, Houwert, R Marijn, Vriens, Menno R, Snijders, Heleen S, Blok, Joris J, Zorgeenheid Traumatologie, Other research (not in main researchprogram), Heelkunde Opleiding, MS CGO, Cancer, Huizing, Floortje, Sier, Vincent Q, Tresfon, Jaco A S, van der Vorst, Joost R, Liem, Ronald S L, Schmitz, Roderick F, Schepers, Abbey, Houwert, R Marijn, Vriens, Menno R, Snijders, Heleen S, and Blok, Joris J

Published
2023

9. The Christmas Procedure: A Surgical Approach to Holiday Cooking.

Author

Sier, Vincent Q., Schepers, Abbey, Dambrauskas, Žilvinas, van der Vorst, Joost R., and Schmitz, Roderick F.

Subjects
*HOLIDAY cooking, *OPERATIVE surgery, *CHRISTMAS, *DUCKS as food, *CHRISTMAS cooking
Abstract

The article discusses how surgeons can apply their skills and mindset to the task of cooking Christmas dinner. Surgeons approach the assembly of the Christmas menu with a willingness to learn and adapt, and they use their organizational skills to manage the preparation and scheduling. They navigate the challenges of shopping for ingredients with ease, and they handle the cooking process with conscientiousness and devotion. The article emphasizes the importance of teamwork and cooperation, and it concludes by reminding readers to be understanding and appreciative of surgeons who take on the task of cooking during the holiday season. [Extracted from the article]

Published
2024
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13. Endoglin/CD105-Based Imaging of Cancer and Cardiovascular Diseases: A Systematic Review

Author

Sier, Vincent Q., primary, van der Vorst, Joost R., additional, Quax, Paul H. A., additional, de Vries, Margreet R., additional, Zonoobi, Elham, additional, Vahrmeijer, Alexander L., additional, Dekkers, Ilona A., additional, de Geus-Oei, Lioe-Fee, additional, Smits, Anke M., additional, Cai, Weibo, additional, Sier, Cornelis F. M., additional, Goumans, Marie José T. H., additional, and Hawinkels, Lukas J. A. C., additional

Published
2021
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14. A multimodal molecular imaging approach targeting urokinase plasminogen activator receptor for the diagnosis, resection and surveillance of urothelial cell carcinoma

Author

Baart, Victor M., primary, van der Horst, Geertje, additional, Deken, Marion M., additional, Bhairosingh, Shadhvi S., additional, Schomann, Timo, additional, Sier, Vincent Q., additional, van der Mark, Maaike H., additional, Iamele, Luisa, additional, de Jonge, Hugo, additional, Resnati, Massimo, additional, Mazar, Andrew P., additional, Pelger, Rob C.M., additional, van der Pluijm, Gabriel, additional, Kuppen, Peter J.K., additional, Vahrmeijer, Alexander L., additional, and Sier, Cornelis F.M., additional

Published
2021
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15. Cell-Based Tracers as Trojan Horses for Image-Guided Surgery

Author

Sier, Vincent Q., primary, de Vries, Margreet R., additional, van der Vorst, Joost R., additional, Vahrmeijer, Alexander L., additional, van Kooten, Cornelis, additional, Cruz, Luis J., additional, de Geus-Oei, Lioe-Fee, additional, Ferreira, Valerie, additional, Sier, Cornelis F. M., additional, Alves, Frauke, additional, and Muthana, Munitta, additional

Published
2021
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