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19 results on '"Siciliano, Veronica"'

6. Fatty Acid Desaturase Involvement in Non-Alcoholic Fatty Liver Disease Rat Models: Oxidative Stress Versus Metalloproteinases

Author

Palladini, Giuseppina, primary, Di Pasqua, Laura, additional, Berardo, Clarissa, additional, Siciliano, Veronica, additional, Richelmi, Plinio, additional, Mannucci, Barbara, additional, Croce, Anna, additional, Rizzo, Vittoria, additional, Perlini, Stefano, additional, Vairetti, Mariapia, additional, and Ferrigno, Andrea, additional

Published
2019
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7. SAT-389-Obeticholic acid reduces matrix metalloproteinases activity via iNOS modulation in hepatic ischemia/reperfusion injury

Author

Pasqua, Laura Giuseppina Di, primary, Palladini, Giuseppina, additional, Berardo, Clarissa, additional, Siciliano, Veronica, additional, Adorini, Luciano, additional, Richelmi, Plinio, additional, Cadamuro, Massimiliano, additional, Fabris, Luca, additional, Perlini, Stefano, additional, Vairetti, Mariapia, additional, and Ferrigno, Andrea, additional

Published
2019
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13. OXIDATIVE STRESS NEGATIVELY AFFECTS DDAH ACTIVITY DESPITE THE INCREASE IN DDAH-1 mRNA IN A RAT MODEL OF NON-ALCOHOLIC STEATOHEPATITIS

Author

Di Pasqua, Laura Giuseppina, Berardo, Clarissa, Siciliano, Veronica, Viscusi, Riccardo, Cozzo, Viviana, Ferrigno, Andrea, Richelmi, Plinio, Croce, Anna Cleta, and Vairetti, Mariapia

Abstract

Objectives: Asymmetric-dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide (NO) synthase, is metabolized by the hepatic enzyme dimethylarginine-dimethylaminohydrolase (DDAH). We studied the impact of oxidative injury on ADMA metabolization, in a rat model of non-alcoholic steatohepatitis (NASH). Methods: We induced NASH in Male Wistar rats using a methionine/choline-deficient˗diet (MCD). Samples were collected during 8 weeks. On samples we assayed hepatic enzymes, ADMA, NAD(P)H autofluorescence, mRNA expression of DDAH and ADMA transporters (CAT-1), DDAH activity, lipid peroxides, glutathione, ATP. Results: DDAH activity decreased and oxidative stress increased, as demonstrated by high lipid peroxidation and low GSH. Curiously, an increase in DDAH-1 mRNA was observed. Low ATP and the NAD(P)H bound/free ratio suggest possible mitochondria alterations (1). Conclusions: These results suggest that oxidative stress might reduce DDAH activity. DDAH is a cysteine hydrolase with an active sh˗ group on the catalytic site, very sensible to oxidation. The decrease in serum ADMA may be due to the diet˗induced reduction of protein methylation and/or to increase in ADMA transporter CAT-1, presumably involved in ADMA excretion. These data suggest a crucial role of free radicals in the reduction of functionality of an hepatic enzyme critical in the control of ADMA levels., Journal of International Society of Antioxidants in Nutrition & Health, Vol 3, No 3 (2016)

Published
2016
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17. Liver Graft Susceptibility during Static Cold Storage and Dynamic Machine Perfusion: DCD versus Fatty Livers.

Author

Ferrigno, Andrea, Di Pasqua, Laura G., Berardo, Clarissa, Siciliano, Veronica, Rizzo, Vittoria, Mannucci, Barbara, Richelmi, Plinio, Croce, Anna Cleta, and Vairetti, Mariapia

Subjects
LIVER transplantation, COLD storage, ORGAN donation, FATTY liver, THERAPEUTICS, LABORATORY rats, REPERFUSION injury
Abstract

We compared static preservation (cold storage, CS, 4 °C) with dynamic preservation (machine perfusion, MP, 20 °C) followed by reperfusion using marginal livers: a model of donation after cardiac death (DCD) livers and two models of fatty livers, the methionine-choline deficient (MCD) diet model, and obese Zucker (fa/fa) rats. CS injury in DCD livers was reversed by an oxygenated washout (OW): hepatic damage, bile flow, and the ATP/ADP ratio in the OW + CS group was comparable with the ratio obtained with MP. Using fatty livers, CS preservation induced a marked release in hepatic and biliary enzymes in obese Zucker rats when compared with the MCD group. The same trend occurred for bile flow. No difference was found when comparing MP in MCD and obese Zucker rats. Fatty acid analysis demonstrated that the total saturated (SFA)/polyunsaturated fatty acid (PUFA) ratio was, respectively, 1.5 and 0.71 in obese Zucker and MCD rats. While preservation damage in DCD livers is associated with the ATP/ADP recovered with OW, injury in fatty livers is linked to fatty acid constituents: livers from obese. Zucker rats, with greater content in saturated FA, might be more prone to CS injury. On the contrary, MCD livers with elevated PUFA content might be less susceptible to hypothermia. [ABSTRACT FROM AUTHOR]

Published
2018
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19. The selective blockade of metabotropic glutamate receptor-5 attenuates fat accumulation in an <em>in vitro</em> model of benign steatosis.

Author

Ferrigno A, Berardo C, Di Pasqua LG, Cagna M, Siciliano V, Richelmi P, and Vairetti M

Subjects
Glycine analogs & derivatives, Glycine pharmacology, Hep G2 Cells, Humans, PPAR alpha metabolism, Receptor, Metabotropic Glutamate 5 agonists, Resorcinols pharmacology, Sterol Regulatory Element Binding Protein 1 metabolism, Fatty Liver drug therapy, Pyridines pharmacology, Receptor, Metabotropic Glutamate 5 antagonists & inhibitors, Triglycerides metabolism
Abstract

It has been previously found that the blockade of metabotropic glutamate receptor type 5 (mGluR5) protects against hepatic ischemia/reperfusion injury and acetaminophen toxicity. The role of mGluR5 in NAFLD has not yet been elucidated. Here, we evaluated the effects of mGluR5 blockade in an in vitro model of steatosis. HepG2 cells were pre-incubated for 12 h with an mGluR5 agonist, a negative allosteric modulator (DHPG and MPEP, respectively) or vehicle, then treated with 1.5 mM oleate/palmitate (O/P) for another 12 h. Cell viability was evaluated with the MTT assay; fat accumulation was measured using the fluorescent dye nile red; SREBP-1, PPAR-α, iNOS and Caspase-3 protein expression were evaluated by Western blot; NFkB activity was evaluated as pNFkB/NFkB ratio. mGluR5 modulation did not alter cell viability in O/P-incubated cells; MPEP prevented intracellular lipid accumulation in O/P treated cells; MPEP administration was also associated with a reversion of O/P-induced changes in SREBP-1 and PPAR-α expression, involved in free fatty acid (FFA) metabolism and uptake. No changes were observed in iNOS and Caspase-3 expression, or in NFkB activity. In conclusion, mGluR5 pharmacological blockade reduced fat accumulation in HepG2 cells incubated with O/P, probably by modulating the expression of SREBP-1 and PPAR-α.

Published
2020
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