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BackgroundWeb-based crowdsourcing promotes the goals achieved effectively by gaining solutions from public groups via the internet, and it has gained extensive attention in both business and academia. As a new mode of sourcing, crowdsourcing has been proven to improve efficiency, quality, and diversity of tasks. However, little attention has been given to crowdsourcing in the health sector. ObjectiveCrowdsourced health care information websites enable patients to post their questions in the question pool, which is accessible to all doctors, and the patients wait for doctors to respond to their questions. Since the sustainable development of crowdsourced health care information websites depends on the participation of the doctors, we aimed to investigate the factors influencing doctors’ participation in providing health care information in these websites from the perspective of the elaboration-likelihood model. MethodsWe collected 1524 questions with complete patient-doctor interaction processes from an online health community in China to test all the hypotheses. We divided the doctors into 2 groups based on the sequence of the answers: (1) doctor who answered the patient’s question first and (2) the doctors who answered that question after the doctor who answered first. All analyses were conducted using the ordinary least squares method. ResultsFirst, the ability of the doctor who first answered the health-related question was found to positively influence the participation of the following doctors who answered after the first doctor responded to the question (βoffline1=.177, P

AIM: To review and summarize the mechanism hypothesis, influencing factors and possible consequences of macular retinal displacement after idiopathic macular hole (IMH) surgery. METHODS: PubMed and Web of Science database was searched for studies published before April 2023 on “Retinal displacement”, “Idiopathic macular holes”, and “Macular displacement”. RESULTS: Recently, more academics have begun to focus on retinal displacement following idiopathic macular holes. They found that internal limiting membrane (ILM) peeling was the main cause of inducing postoperative position shift in the macular region. Moreover, several studies have revealed that the macular hole itself, as well as ILM peeling method, will have an impact on the result. In addition, this phenomenon is related to postoperative changes in macular retinal thickness, cone outer segment tips line recovery, the occurrence of dissociated optic nerve fiber layer (DONFL) and the degree of metamorphopsia. CONCLUSION: As a subclinical phenomenon, the clinical significance of postoperative macular displacement cannot be underestimated as it may affect the recovery of anatomy and function.

With the continuous development of economy and industry, the demand for energy in modern society is ever-increasing. As a kind of high efficiency and low carbon energy, nuclear energy has attracted more and more attention. Developing nuclear energy, however, also brings some problems such as the release of radioactive metal ions that pose a threat to the environment and human health. Therefore, separation and removal of radioactive ions(e.g., U(Ⅵ)) from wastewater is of great significance to ensure the sustainable development of nuclear energy. Covalent organic frameworks(COFs) are a kind of periodic porous polymer materials composed of organic molecular units connected by covalent bonds. The emergence of COFs marks a new era in the field of materials. Because of the design ability of COFs and its unique advantages in structure and pore, it shows a good application prospect in catalysis, sensing and adsorption. Very recent efforts by some investigators have focused on the application of COFs in adsorption and separation of radionuclides, generally with encouraging results. According to the classification from the structural dimension, COFs can be divided into two-dimensional COFs materials(2D-COFs) and three-dimensional COFs materials(3D-COFs). Up to now, 2D COFs materials have been widely studied and applied. In contrast, the development of 3D-COF has been relatively slow, and the performance and application have been limited, a result of the limitations of molecular structural units and topological connections, as well as the difficulties of synthesis and structural analysis. To this end, in this paper, a combination of bond transformation and post-grafting was used to construct functional 3D-COFs materials for the efficient removal of U(Ⅵ) from wastewater. Firstly, the imine bond was reduced to an irreversible amine bond by reduction reaction, and then sulfonic acid group was introduced into the skeleton by reaction with 1, 3-propyl sulfonolactone to successfully synthesize COF-320-H2SO3. The structure and properties of COF-320-H2SO3 were characterized by various characterization methods, and the adsorption behavior of COF-320-H2SO3 on U(Ⅵ) was systematically studied. The results show that the chemical stability of COFs is significantly improved by the conversion of imine bond to amine bond, and the adsorption capacity of the material for U(Ⅵ) is clearly enhanced by the introduction of sulfonic acid group with hydrophilic and strong coordination ability. Even under the conditions of 3 mol/L HNO3, the adsorption capacity of U(Ⅵ) can reach more than 40 mg/g. This work further reveals the potential application value of COFs, especially 3D-COFs materials, in the separation of radioactive metal ions.

Aim To optimize gastric cancer screening score and reduce screening costs using machine learning models. Methods This study included 228,634 patients from the Taizhou Gastric Cancer Screening Program. We used three machine learning models to optimize Li's gastric cancer screening score: Gradient Boosting Machine (GBM), Distributed Random Forest (DRF), and Deep Learning (DL). The performance of the binary classification models was evaluated using the area under the curve (AUC) and area under the precision–recall curve (AUCPR). Results In the binary classification model used to distinguish low-risk and moderate- to high-risk patients, the AUC in the GBM, DRF, and DL full models were 0.9994, 0.9982, and 0.9974, respectively, and the AUCPR was 0.9982, 0.9949, and 0.9918, respectively. Excluding Helicobacter pylori IgG antibody, pepsinogen I, and pepsinogen II, the AUC in the GBM, DRF, and DL models were 0.9932, 0.9879, and 0.9900, respectively, and the AUCPR was 0.9835, 0.9716, and 0.9752, respectively. Remodel after removing variables IgG, PGI, PGII, and G-17, the AUC in GBM, DRF, and DL was 0.8524, 0.8482, 0.8477, and AUCPR was 0.6068, 0.6008, and 0.5890, respectively. When constructing a tri-classification model, we discovered that none of the three machine learning models could effectively distinguish between patients at intermediate and high risk for gastric cancer (F1 scores in the GBM model for the low, medium and high risk: 0.9750, 0.9193, 0.5334, respectively; F1 scores in the DRF model for low, medium, and high risks: 0.9888, 0.9479, 0.6694, respectively; F1 scores in the DL model for low, medium, and high risks: 0.9812, 0.9216, 0.6394, respectively). Conclusion We concluded that gastric cancer screening indicators could be optimized when distinguishing low-risk and moderate to high-risk populations, and detecting gastrin-17 alone can achieve a good discriminative effect, thus saving huge expenditures.

The five-year survival rate for patients with hepatocellular carcinoma (HCC) is only 20 %, highlighting the urgent need to identify new therapeutic targets and develop potential therapeutic options to improve patient prognosis. One promising approach is inhibiting autophagy as a strategy for HCC treatment. In this study, we established a virtual docking conformation of the autophagy promoter ULK1 binding XST-14 derivatives. Based on this conformation, we designed and synthesized four series of derivatives. By evaluating their affinity and anti-HCC effects, we confirmed that these compounds exert anti-HCC activity by inhibiting ULK1. The structure-activity relationship was summarized, with derivative A4 showing 10 times higher activity than XST-14 and superior efficacy to sorafenib against HCC. A4 has excellent effect on reducing tumor growth and enhancing sorafenib activity in HepG2 and HCCLM3 cells. Moreover, we verified the therapeutic effect of A4 in sorafenib-resistant HCC cells both in vivo and in vitro. These results suggest that inhibiting ULK1 to regulate autophagy may become a new treatment method for HCC and that A4 will be used as a lead drug for HCC in further research. Overall, A4 shows good drug safety and efficacy, offering hope for prolonging the survival of HCC patients.

Public participation in architectural heritage protection is a crucial part of promoting the development of heritage conservation. However, even in the same country, different regions protect heritage at different levels. This study, taking national wooden arch corridor bridges as the research object, analyzes the public participation level in wooden arch corridor bridge protection in the last 5 years, studying the factors that influence public participation and the change in rules of public participation caused by geography, providing a theoretical reference for the sustainable development of the protection of wooden arch corridor bridges. The results indicate (1) public participation in the protection of wooden arch corridor bridges has been increasing annually under government guidance; (2) there is a positive correlation between the concentration of wooden arch corridor bridges and the level of public participation; (3) the better the planning is regarding wooden arch corridor bridges and their surrounding areas, the higher the level of public participation, while the lower the level of residents’ participation and willing; (4) the higher the development and better the transportation in the areas where wooden arch corridor bridges are located, the higher the level of public participation; and (5) increasing the popularity of wooden arch corridor bridges, as well as the areas they are located in, can enhance public participation. These conclusions and research methods also provide methodological guidance and theoretical support for other public participation in the study of architectural heritage.

PAMP-triggered immunity (PTI) is the first layer of plant defense response that occurs on the plant plasma membrane. Recently, the application of a rhizobacterium, Bacillus amyloliquefaciens strain PMB05, has been demonstrated to enhance flg22Pst- or harpin-triggered PTI response such as callose deposition. This PTI intensification by PMB05 further contributes to plant disease resistance to different bacterial diseases. Under the demand for rapid and large-scale screening, it has become critical to establish a non-staining technology to identify microbial strains that can enhance PTI responses. Firstly, we confirmed that the expression of the GSL5 gene, which is required for callose synthesis, can be enhanced by PMB05 during PTI activation triggered by flg22 or PopW (a harpin from Ralstonia solanacearum). The promoter region of the GSL5 gene was further cloned and fused to the coding sequence of gfp. The constructed fragments were used to generate transgenic Arabidopsis plants through a plant transformation vector. The transgenic lines of AtGSL5-GFP were obtained. The analysis was performed by infiltrating flg22Pst or PopW in one homozygous line, and the results exhibited that the green fluorescent signals were observed until after 8 h. In addition, the PopW-induced fluorescent signal was significantly enhanced in the co-treatment with PMB05 at 4 h after inoculation. Furthermore, by using AtGSL5-GFP to analyze 13 Bacillus spp. strains, the regulation of PopW-induced fluorescent signal was observed. And, the regulation of these fluorescent signals was similar to that performed by callose staining. More importantly, the Bacillus strains that enhance PopW-induced fluorescent signals would be more effective in reducing the occurrence of bacterial wilt. Taken together, the technique by using AtGSL5-GFP would be a promising platform to screen plant immunity-intensifying microbes to control bacterial wilt.

Laminaria japonica (LJ) is a common seaweed, which has been widely used as functional food and medicine, but its pharmaceutical activities in angiogenic regulation are unclear. Here, different lines of studies provide evidence to the suppressive effects of LJ extract in VEGF-medicated angiogenic function. In cellular level, LJ extract significantly inhibited VEGF-mediated endothelial cell proliferation, migration, invasion and tube formation. For in vivo verification, LJ extract suppressed the VEGF-induced formation of sub-intestinal vessels in zebrafish embryo model. In signalling cascades, LJ extract decreased the amount of phosphorylated VEGF receptor-2, as triggered by VEGF, and subsequently some key related downstream elements were obviously inactivated. In parallel, NO production and ROS formation, as induced by VEGF, were significantly attenuated. Thus, these preclinical findings promoted the possible development of LJ extract as a potential therapeutic agent for prevention and clinical treatment of angiogenesis-related diseases.

Hepatocellular carcinoma (HCC) is one of the most fatal solid malignancies worldwide. Evidence suggests that thrombin stimulates tumor progression via fibrin formation and platelet activation. Meanwhile, we also found a correlation between thrombin and HCC through bioinformatics analysis. Dabigatran is a selective, direct thrombin inhibitor that reversibly binds to thrombin. Dabigatran was used as the lead agent in this study, and 19 dabigatran derivatives were designed and synthesized based on docking mode. The thrombin-inhibitory activity of the derivative AX-2 was slightly better than that of dabigatran. BX-2, a prodrug of AX-2, showed a fairly strong inhibitory effect on thrombin-induced platelet aggregation, and effectively antagonized proliferation of HCC tumor cells induced by thrombin at the cellular level. Furthermore, BX-2 reduced tumor volume, weight, lung metastasis, and secondary tumor occurrence in nude mouse models. BX-2 combined with sorafenib increased sorafenib efficacy. This study lays the foundation for discovering new anti-HCC mechanism based on thrombin. BX-2 can be used as an anti-HCC drug lead for further research.