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Abstract Background The study was conducted to assess the characterization, differentiation, and in vitro cell regeneration potential of canine mesenteric white adipose tissue-derived mesenchymal stem cells (AD-MSCs). The tissue was harvested through surgical incision and digested with collagenase to obtain a stromal vascular fraction. Mesenchymal stem cells isolated from the stromal vascular fraction were characterized through flow cytometry and reverse transcription-polymerase chain reaction. Assessment of cell viability, in vitro cell regeneration, and cell senescence were carried out through MTT assay, wound healing assay, and β-galactosidase assay, respectively. To ascertain the trilineage differentiation potential, MSCs were stained with alizarin red for osteocytes, alcian blue for chondrocytes, and oil o red for adipocytes. In addition, differentiated cells were characterized through a reverse transcription-polymerase chain reaction. Results We observed the elongated, spindle-shaped, and fibroblast-like appearance of cells after 72 h of initial culture. Flow cytometry results showed positive expression for CD44, CD90, and negative expression for CD45 surface markers. Population doubling time was found 18–24 h for up to the fourth passage and 30±0.5 h for the fifth passage. A wound-healing assay was used to determine cell migration rate which was found 136.9 ± 4.7 μm/h. We observed long-term in vitro cell proliferation resulted in MSC senescence. Furthermore, we also found that the isolated cells were capable of differentiating into osteogenic, chondrogenic, and adipogenic lineages. Conclusions Mesenteric white adipose tissue was found to be a potential source for isolation, characterization, and differentiation of MSCs. This study might be helpful for resolving the problems regarding the paucity of information concerning the basic biology of stem cells. The large-scale use of AD-MSCs might be a remedial measure in regenerative medicine.

The present study was carried out to evaluate the biomarkers of oxidative stress and antioxidant activity in female dogs undergoing laparoscopic and open elective ovariectomy at Bihar Veterinary College, Patna in 2016-17. Twenty healthy animals were randomly divided into four groups, viz. A, B, C and D consisting of 5 animals each. The ovariectomy was performed through laparoscopy in Group A and Group B. Open elective ovariectomy procedure was used in Group C and Group D. Evaluation of surgical techniques was done on the basis of biomarkers for oxidative stress and antioxidant activity at Pre, Post and 4th day post surgery. The superoxide dismutase level on day 4th in Group B showed significant difference with highest value followed by Groups A, C and D respectively. The catalase levels on day 4th in Group B were significantly different from Groups A and D but not from Group C. The values within the groups were significantly higher on day 4th as compared to pre and post intervals of time. It can be concluded that less oxidative stress is induced during surgical procedure by laparoscopy as compared to open laparotomy ovariectomy in canines.