67 results on '"Shuping Qu"'
Search Results
2. Morphological Analyses and QTL Mapping of Mottled Leaf in Zucchini (Cucurbita pepo L.)
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Kexin Wang, Xinyu Wang, Lijing Zhang, Yichen Chi, Yusong Luo, Wenlong Xu, Yunli Wang, and Shuping Qu
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Cucurbita pepo L. ,QTL analysis ,mottled leaf ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The mottled leaf is one of the agronomic traits of zucchini and can be applied as a marker trait in aggregation breeding. However, the genetic mechanism responsible for mottled leaf has yet to be elucidated. In the present study, we used two inbred lines (line ‘19’: silver mottled leaf; line ‘113’: normal leaf) as parents for the physiological and genetic analysis of mottled leaf. The synthesis and net photosynthetic rate of chlorophyll were not significantly affected in the mottled areas of leaves. However, we detected a large space between the palisade parenchyma in the leaf mottle area of line ‘19’, which may have caused the mottled leaf phenotype. Light also plays an important role in the formation of mottled leaf, and receiving light during the early stages of leaf development is a necessary factor. Genetic analysis has previously demonstrated that mottled leaf is a quantitative trait that is controlled by multiple genes. Based on the strategy of quantitative trait locus sequencing (QTL-seq), two QTLs were identified on chromosomes 1 and 17, named CpML1.1 and CpML17.1, respectively. Two major loci were identified using R/qtl software version 1.66 under greenhouse conditions in April 2019 (2019A) and April 2020 (2020A) and under open cultivation conditions in May 2020 (2020M). The major QTL, CpML1.1, was located in a 925.2-kb interval on chromosome 1 and explained 10.51%-24.15% of the phenotypic variation. The CpML17.1 was located in a 719.7-kb interval on chromosome 17 and explained 16.25%-38.68% of the phenotypic variation. Based on gene annotation, gene sequence alignment, and qRT–PCR analysis, the Cp4.1LG01g23790 at the CpML1.1 locus encoding a protein of the TPX2 family (target protein of Xklp2) may be a candidate gene for mottled leaf in zucchini. Our findings may provide a theoretical basis for the formation of mottled leaf and provide a foundation for the fine mapping of genes associated with mottled leaf. Molecular markers closely linked to mottled leaf can be used in molecular-assisted selection for the zucchini mottled leaf breeding.
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- 2024
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3. Neutrophil-to-Lymphocyte Ratio and Early Tumor Shrinkage as Predictive Biomarkers in Unresectable Hepatocellular Carcinoma Patients Treated With Lenvatinib, PD-1 Inhibitors, in Combination With TACE
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Shuping Qu MD, Dong Wu PhD, and Zhiming Hu PhD
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Purpose: The purpose of this prospective observational study was to investigate the relationship between pretreatment neutrophil-to-lymphocyte ratio (NLR) and posttreatment early tumor shrinkage (ETS), and clinical outcomes in patients with unresectable hepatocellular carcinoma (uHCC) who received lenvatinib, programmed death-1 inhibitors plus transcatheter arterial chemoembolization. Patients and Methods: A total of 63 uHCC patients were treated with this triple combination. Multivariate analyses to determine the independent factors associated with overall survival (OS) were employed. The link between NLR and clinical results was further analyzed. Furthermore, the predictive value of combining NLR with ETS should be investigated to stratify patients receiving treatment for survival benefits. Results: Progression-free survival and OS were 9.8 and 23.0 months, respectively, with a median follow-up of 20.8 months. On a multivariate analysis of OS, NLR was the only independent prognostic factor. Patients with NLR low (NLR
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- 2023
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4. QTL mapping and stability analysis of trichome density in zucchini (Cucurbita pepo L.)
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Yunli Wang, Guichao Wang, Dongjuan Lin, Qinfen Luo, Wenlong Xu, and Shuping Qu
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Cucurbita pepo ,trichome density ,InDel markers ,QTL analysis ,zinc finger protein ,Plant culture ,SB1-1110 - Abstract
Trichomes provide an excellent model for studying cell differentiation and proliferation. The aboveground tissues of plants with long dense trichomes (LDTs) can cause skin itching in people working in a zucchini field, in which management, pollination, and fruit harvesting are difficult. In this study, an F2 population was constructed with the LDT inbred line “16” and the sparse micro trichome (SMT) inbred line “63” for QTL analysis of type I and II trichome density. Two QTLs were identified on chromosomes 3 and 15 using the QTL-seq method. Additionally, 191 InDel markers were developed on 20 chromosomes, a genetic map was constructed for QTL mapping, and three QTLs were identified on chromosomes 3, 6, and 15. Two QTLs, CpTD3.1 and CpTD15.1, were identified in both QTL-seq and genetic map-based QTL analyses, and CpTD15.1 was the major-effect QTL. The stability of CpTD3.1 and CpTD15.1 was confirmed using data from F2 plants under different environmental conditions. The major-effect QTL CpTD15.1 was located between markers chr15-4991349 and chr15-5766791, with a physical distance of 775.44 kb, and explained 12.71%–29.37% of the phenotypic variation observed in the three environments. CpTD3.1 was located between markers chr3-218350 and chr3-2891236, in a region with a physical distance of 2,672.89 kb, and explained 5.00%–10.64% of the phenotypic variation observed in the three environments. The functional annotations of the genes within the CpTD15.1 region were predicted, and five genes encoding transcription factors regulating trichome development were selected. Cp4.1LG15g04400 encoded zinc finger protein (ZFP) and harbored nonsynonymous SNPs in the conserved ring finger domain between the two parental lines. There were significant differences in Cp4.1LG15g04400 expression between “16” and “63”, and a similar pattern was found between germplasm resources of LDT lines and SMT lines. It was presumed that Cp4.1LG15g04400 might regulate trichome density in zucchini. These results lay a foundation for better understanding the density of multicellular nonglandular trichomes and the regulatory mechanism of trichome density in zucchini.
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- 2023
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5. Single cell transcriptional diversity and intercellular crosstalk of human liver cancer
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Yan Meng, Yan Sang, Jianping Liao, Qiudong Zhao, Shuping Qu, Rong Li, Jinghua Jiang, Meifeng Wang, Jiahong Wang, Dong Wu, Chun Cheng, and Lixin Wei
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Cytology ,QH573-671 - Abstract
Abstract Liver cancer arises from the evolutionary selection of the dynamic tumor microenvironment (TME), in which the tumor cell generally becomes more heterogeneous; however, the mechanisms of TME-mediated transcriptional diversity of liver cancer remain unclear. Here, we assess transcriptional diversity in 15 liver cancer patients by single-cell transcriptome analysis and observe transcriptional diversity of tumor cells is associated with stemness in liver cancer patients. Tumor-associated fibroblast (TAF), as a potential driving force behind the heterogeneity in tumor cells within and between tumors, was predicted to interact with high heterogeneous tumor cells via COL1A1-ITGA2. Moreover, COL1A1-mediated YAP-signaling activation might be the mechanistic link between TAF and tumor cells with increased transcriptional diversity. Strikingly, the levels of COL1A1, ITGA2, and YAP are associated with morphological heterogeneity and poor overall survival of liver cancer patients. Beyond providing a potential mechanistic link between the TME and heterogeneous tumor cells, this study establishes that collagen-stimulated YAP activation is associates with transcriptional diversity in tumor cells by upregulating stemness, providing a theoretical basis for individualized treatment targets.
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- 2022
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6. Accumulation of carotenoids and expression of carotenoid biosynthesis genes in fruit flesh during fruit development in two Cucurbita maxima inbred lines
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Yusong Luo, Chaojie Wang, Manman Wang, Yunli Wang, Wenlong Xu, Hongyu Han, Zhichao Wang, Yujuan Zhong, Hexun Huang, and Shuping Qu
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Cucurbita maxima ,Flesh color ,Lutein ,β-carotene ,Zeaxanthin ,Plant culture ,SB1-1110 - Abstract
ABSTRACT: Mesocarp color is an important agronomic trait of Cucurbita maxima and is determined mainly by the contents and compositions of the carotenoids. The two inbred lines with significant differences in fruit flesh color were used in the study, the orange ‘312-1’ and white ‘98-2’. Changes in seven carotenoid contents and compositions in the flesh of fruit produced by inbred lines ‘312-1’ and ‘98-2’ were analyzed during fruit development. The expression of eight key carotenoid biosynthesis genes in the fruit flesh were investigated during five fruit development stage in two inbred lines. As the flesh color intensified, the orange flesh color of ‘312-1’ was determined mainly by the increased contents of lutein, β-carotene and zeaxanthin and the lack of carotenoid accumulation led to the formation of white flesh in ‘98-2’ fruit. The expression of the LCY-e and CHYb genes was significantly stronger in ‘312-1’ than in ‘98-2’, and their expression was strongly correlated with lutein and β-carotene contents during fruit development. This study provides a deep understanding of the molecular mechanism of carotenoid biosynthesis in fruit flesh and provides a basis for additional studies on the highly refined improvement of squash quality.
- Published
- 2021
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7. Antioxidant and C5a-blocking strategy for hepatic ischemia–reperfusion injury repair
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Xiaobing Zhang, Jiajia Hu, Kaelyn V. Becker, Jonathan W. Engle, Dalong Ni, Weibo Cai, Dong Wu, and Shuping Qu
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Hepatic ischemia–reperfusion injury ,Nanoantioxidants ,C5a ,Nanoceria ,Aptamer ,Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
Abstract Background Nonspecific liver uptake of nanomaterials after intravenous injection has hindered nanomedicine for clinical translation. However, nanomaterials’ propensity for liver distribution might enable their use in hepatic ischemia–reperfusion injury (IRI) repair. During hepatic IRI, reactive oxygen species (ROS) are generated and the fifth component of complement (C5a) is activated. In addition, C5a is confirmed to exacerbate the vicious cycle of oxidative stress and inflammatory damage. For these reasons, we have investigated the development of nanomaterials with liver uptake to scavenge ROS and block C5a for hepatic IRI repair. Results To achieve this goal, a traditional nanoantioxidant of nanoceria was surface conjugated with the anti-C5a aptamers (Ceria@Apt) to scavenge the ROS and reduce C5a-mediated inflammation. High uptake of Ceria@Apt in the liver was confirmed by preclinical positron emission tomography (PET) imaging. The clinical symptoms of hepatic IRI were effectively alleviated by Ceria@Apt with ROS scavenging and C5a blocking in mice model. The released pro-inflammatory cytokines were significantly reduced, and subsequent inflammatory reaction involved in the liver was inhibited. Conclusions The synthesized Ceria@Apt has great potential of medical application in hepatic IRI repair, which could also be applied for other ischemic-related diseases. Graphic abstract
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- 2021
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8. Efficacy and Safety of TACE Combined With Lenvatinib Plus PD-1 Inhibitors Compared With TACE Alone for Unresectable Hepatocellular Carcinoma Patients: A Prospective Cohort Study
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Shuping Qu, Xiaobing Zhang, Yutian Wu, Yan Meng, Hongyu Pan, Qiang Fang, Lei Hu, Jin Zhang, Ruoyu Wang, Lixin Wei, and Dong Wu
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hepatocellular carcinoma ,transcatheter arterial chemoembolization ,lenvatinib ,programmed cell death-1 inhibitor ,neutrophil-to-lymphocyte ratio ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
PurposeTo compare the efficacy and safety of the combination of transcatheter arterial chemoembolization (TACE), Lenvatinib, and programmed cell death protein-1 (PD-1) inhibitors (combination group) with TACE (TACE group) in the treatment of patients with unresectable hepatocellular carcinoma (uHCC).MethodsWe consecutively enrolled 110 patients with uHCC in this prospective cohort study, with 56 patients receiving combination treatment and 54 patients receiving TACE from November 2017 to September 2020. The differences in tumor response, survival benefit, and adverse events (AEs) were compared between the two groups. Factors affecting survival were identified via Cox regression analysis.ResultsCompared with the TACE group, the combination group had a higher objective response rate (ORR) (67.9% vs. 29.6%, p < 0.001), longer median progression-free survival (mPFS) (11.9 vs. 6.9 months, P = 0.003) and overall survival (mOS) (23.9 vs. 15.3 months, p < 0.001). Multivariate analysis showed that the neutrophil-to-lymphocyte ratio (NLR) and the treatment option were independent factors associated with the PFS and OS. Further subgroup analysis showed that patients with low NLR (≤median 3.11) receiving combination therapy had better mPFS (20.1 vs. 6.2 months, P < 0.001) and mOS (28.9 vs. 15.2 months, P < 0.001) than those receiving TACE, while no obvious difference in PFS or OS was observed between the two groups in patients with high NLR (> 3.11). There were no unexpected toxicities in the combination group.ConclusionCompared with TACE, the combination treatment demonstrated an improved clinical efficacy and manageable safety profile in patients with uHCC. Combination treatment showed better therapeutic efficacy in patients with low NLR; therefore, this ratio could be used to identify patients who will benefit from this treatment.
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- 2022
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9. Genome-Wide Identification and Characterization of Members of the ACS Gene Family in Cucurbita maxima and Their Transcriptional Responses to the Specific Treatments
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Chaojie Wang, Wenling Li, Fangyuan Chen, Yaqian Cheng, Xin Huang, Bingxue Zou, Yunli Wang, Wenlong Xu, and Shuping Qu
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ACS gene family ,Cucurbita maxima ,sex determination ,flower development ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Ethylene biosynthesis and signal transduction play critical roles in plant sex differentiation. ACS (1-aminocyclopropane-1-carboxylic acid synthase) is a rate-limiting enzyme in ethylene biosynthesis. However, the understanding of the ACS gene family in Cucurbita maxima is limited. Here, we identified and characterized 13 ACS genes in the C. maxima genome. All ACS genes could be divided into three groups according to a conserved serine residue at the C-terminus. Thirteen CmaACS genes were found to be randomly distributed on 10 of the 20 chromosomes of C. maxima. The ACS gene exhibits different tissue-specific expression patterns in pumpkin, and four ACS genes (CmaACS1, CmaACS4, CmaACS7, and CmaACS9) were expressed specifically in both the female and male flowers of C. maxima. In addition, the expression levels of CmaACS4 and CmaACS7 were upregulated after ethephon and IAA treatments, which ultimately increased the number of female flowers, decreased the position of the first female flower and decreased the number of bisexual flowers per plant. These results provide relevant information for determining the function of the ACS genes in C. maxima, especially for regulating the function of ethylene in sex determination.
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- 2022
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10. An 8 miRNA-Based Risk Score System for Predicting the Prognosis of Patients With Papillary Thyroid Cancer
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Wanwan Yi MM, Jin Liu BM, Shuping Qu MM, Hengwei Fan MM, and Zhongwei Lv MD
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background: Dysregulation of microRNAs (miRNAs) in papillary thyroid cancer (PTC) might influence prognosis of PTC. This study is aimed to develop a risk score system for predicting prognosis of PTC. Methods: The miRNA and gene expression profiles of PTC were obtained from The Cancer Genome Atlas database. PTC samples were randomly separated into training set (n = 248) and validation set (n = 248). The differentially expressed miRNAs (DE-miRNAs) in the training set were screened using limma package. The independent prognosis-associated DE-miRNAs were identified for building a risk score system. Risk score of PTC samples in the training set was calculated and samples were divided into high risk group and low risk group. Kaplan-Meier curves and receiver operating characteristic (ROC) curve were used to assess the accuracy of the risk score system in the training set, validation set and entire set. Finally, a miRNA-gene regulatory network was visualized by Cytoscape software, followed by enrichment analysis. Results: Totally, 162 DE-miRNAs between tumor and control groups in the training set were identified. An 8 independent prognosis-associated DE-miRNAs, (including miR-1179, miR-133b, miR-3194, miR-3912, miR-548j, miR-6720, miR-6734, and miR-6843) based risk score system was developed. The area under ROC curve in the training set, validation set and entire set was all above 0.93. A miRNA-gene regulatory network involving the 8 DE-miRNAs were built and functional enrichment analysis suggested the genes in the network were significantly enriched into 13 pathways, including calcium signaling pathway and hedgehog signaling pathway. Conclusion: The risk score system developed this study might be used for predicting the prognosis of PTC. Besides, the 8 miRNAs might affect the prognosis of PTC via hedgehog signaling pathway and calcium signaling pathway.
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- 2020
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11. Weighted Gene Coexpression Network Analysis Reveals the Dynamic Transcriptome Regulation and Prognostic Biomarkers of Hepatocellular Carcinoma
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Shuping Qu, Qiuyuan Shi, Jing Xu, Wanwan Yi, and Hengwei Fan
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Evolution ,QH359-425 - Abstract
This study was aimed at revealing the dynamic regulation of mRNAs, long noncoding RNAs (lncRNAs), and microRNAs (miRNAs) in hepatocellular carcinoma (HCC) and to identify HCC biomarkers capable of predicting prognosis. Differentially expressed mRNAs (DEmRNAs), lncRNAs, and miRNAs were acquired by comparing expression profiles of HCC with normal samples, using an expression data set from The Cancer Genome Atlas. Altered biological functions and pathways in HCC were analyzed by subjecting DEmRNAs to Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis. Gene modules significantly associated with disease status were identified by weighted gene coexpression network analysis. An lncRNA-mRNA and an miRNA-mRNA coexpression network were constructed for genes in disease-related modules, followed by the identification of prognostic biomarkers using Kaplan-Meier survival analysis. Differential expression and association with the prognosis of 4 miRNAs were verified in independent data sets. A total of 1220 differentially expressed genes were identified between HCC and normal samples. Differentially expressed mRNAs were significantly enriched in functions and pathways related to “plasma membrane structure,” “sensory perception,” “metabolism,” and “cell proliferation.” Two disease-associated gene modules were identified. Among genes in lncRNA-mRNA and miRNA-mRNA coexpression networks, 9 DEmRNAs and 7 DEmiRNAs were identified to be potential prognostic biomarkers. MIMAT0000102, MIMAT0003882, and MIMAT0004677 were successfully validated in independent data sets. Our results may advance our understanding of molecular mechanisms underlying HCC. The biomarkers may contribute to diagnosis in future clinical practice.
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- 2020
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12. Quantitative Trait Loci for Seed Size Variation in Cucurbits – A Review
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Yu Guo, Meiling Gao, Xiaoxue Liang, Ming Xu, Xiaosong Liu, Yanling Zhang, Xiujie Liu, Jixiu Liu, Yue Gao, Shuping Qu, and Feishi Luan
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cucurbits ,watermelon ,pumpkin/squash ,cucumber ,melon ,seed size ,Plant culture ,SB1-1110 - Abstract
Cucurbits (Cucurbitaceae family) include many economically important fruit vegetable crops such as watermelon, pumpkin/squash, cucumber, and melon. Seed size (SS) is an important trait in cucurbits breeding, which is controlled by quantitative trait loci (QTL). Recent advances have deciphered several signaling pathways underlying seed size variation in model plants such as Arabidopsis and rice, but little is known on the genetic basis of SS variation in cucurbits. Here we conducted literature review on seed size QTL identified in watermelon, pumpkin/squash, cucumber and melon, and inferred 14, 9 and 13 consensus SS QTL based on their physical positions in respective draft genomes. Among them, four from watermelon (ClSS2.2, ClSS6.1, ClSS6.2, and ClSS8.2), two from cucumber (CsSS4.1 and CsSS5.1), and one from melon (CmSS11.1) were major-effect, stable QTL for seed size and weight. Whole genome sequence alignment revealed that these major-effect QTL were located in syntenic regions across different genomes suggesting possible structural and functional conservation of some important genes for seed size control in cucurbit crops. Annotation of genes in the four watermelon consensus SS QTL regions identified genes that are known to play important roles in seed size control including members of the zinc finger protein and the E3 ubiquitin-protein ligase families. The present work highlights the utility of comparative analysis in understanding the genetic basis of seed size variation, which may help future mapping and cloning of seed size QTL in cucurbits.
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- 2020
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13. Construction of a High-Density Genetic Map and Analysis of Seed-Related Traits Using Specific Length Amplified Fragment Sequencing for Cucurbita maxima
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Yunli Wang, Chaojie Wang, Hongyu Han, Yusong Luo, Zhichao Wang, Chundong Yan, Wenlong Xu, and Shuping Qu
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Cucurbita maxima ,high-density genetic map ,specific length amplified fragment sequencing ,seed-related traits ,cleaved amplified polymorphic sequence ,Plant culture ,SB1-1110 - Abstract
Seed traits are agronomically important for Cucurbita breeding, but the genes controlling seed size, seed weight and seed number have not been mapped in Cucurbita maxima (C. maxima). In this study, 100 F2 individual derived from two parental lines, “2013-12” and “9-6”, were applied to construct a 3,376.87-cM genetic map containing 20 linkage groups (LGs) with an average genetic distance of 0.47 cM using a total of 8,406 specific length amplified fragment (SLAF) markers in C. maxima. Ten quantitative trait loci (QTLs) of seed width (SW), seed length (SL) and hundred-seed weight (HSW) were identified using the composite interval mapping (CIM) method. The QTLs affecting SW, SL and HSW explained a maximum of 38.6%, 28.9% and 17.2% of the phenotypic variation and were detected in LG6, LG6 and LG17, respectively. To validate these results, an additional 150 F2 individuals were used for QTL mapping of SW and SL with cleaved amplified polymorphic sequence (CAPS) markers. We found that two major QTLs, SL6-1 and SW6-1, could be detected in both SLAF-seq and CAPS markers in an overlapped region. Based on gene annotation and non-synonymous single-nucleotide polymorphisms (SNPs) in the major SWand SL-associated regions, we found that two genes encoding a VQ motif and an E3 ubiquitin-protein ligase may be candidate genes influencing SL, while an F-box and leucinerich repeat (LRR) domain-containing protein is the potential regulator for SW in C. maxima. This study provides the first high-density linkage map of C. maxima using SNPs developed by SLAF-seq technology, which is a powerful tool for associated mapping of important agronomic traits, map-based gene cloning and marker-assisted selection (MAS)-based breeding in C. maxima.
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- 2020
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14. Morphological, Transcriptomic and Hormonal Characterization of Trimonoecious and Subandroecious Pumpkin (Cucurbita maxima) Suggests Important Roles of Ethylene in Sex Expression
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Yunli Wang, Chundong Yan, Bingxue Zou, Chaojie Wang, Wenlong Xu, Chongshi Cui, and Shuping Qu
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Cucurbita maxima ,transcriptome sequencing ,floral sex expression ,ethylene signal synthesis and transduction ,chemical treatment ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Sex expression is a complex process, and in-depth knowledge of its mechanism in pumpkin is important. In this study, young shoot apices at the one-true-leaf stage and 10-leaf stage in Cucurbita maxima trimonoecious line ‘2013−12’ and subandroecious line ‘9−6’ were collected as materials, and transcriptome sequencing was performed using an Illumina HiSeqTM 2000 System. 496 up-regulated genes and 375 down-regulated genes were identified between shoot apices containing mostly male flower buds and only female flower buds. Based on gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, the differentially expressed genes were mainly enriched in the ethylene and auxin synthesis and signal transduction pathways. In addition, shoot apices at the 4-leaf stage were treated with the ethylene-releasing agent 2-chloroethylphosphonic acid (Ethrel), aminoethoxyvinyl glycine (AVG), AgNO3 and indoleacetic acid (IAA). The number of female flowers up to node 20 on the main stem of ‘2013−12’ increased significantly after Ethrel and IAA treatment and decreased significantly after AVG and AgNO3 treatment. The female flowers in ‘9−6’ showed slight changes after treatment with the exogenous chemicals. The expression of key genes in ethylene synthesis and signal transduction (CmaACS7, CmaACO1, CmaETR1 and CmaEIN3) was determined using quantitative RT-PCR, and the expression of these four genes was positively correlated with the number of female flowers in ‘2013−12’. The variations in gene expression, especially that of CmaACS7, after chemical treatment were small in ‘9−6’. From stage 1 (S1) to stage 7 (S7) of flower development, the expression of CmaACS7 in the stamen was much lower than that in the ovary, stigma and style. These transcriptome data and chemical treatment results indicated that IAA might affect pumpkin sex expression by inducing CmaACS7 expression and indirectly affecting ethylene production, and the ethylene synthesis and signal transduction pathways play crucial roles in pumpkin flower sex expression. A possible reason for the differences in sex expression between pumpkin lines ‘2013−12’ and ‘9−6’ was proposed based on the key gene expression. Overall, these transcriptome data and chemical treatment results suggest important roles for ethylene in pumpkin sex expression.
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- 2019
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15. Classification of inheritance of trichomes in different organs in zucchini ( Cucurbita pepo L.)
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Dongjuan Lin, Yusong Luo, Wenling Li, Yuetong Hou, Wenqi Ding, Chaojie Wang, Feng Zhang, Shuping Qu, and Yunli Wang
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Agronomy and Crop Science - Published
- 2023
16. Accumulation of carotenoids and expression of carotenoid biosynthesis genes in fruit flesh during fruit development in two Cucurbita maxima inbred lines
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Yunli Wang, Yusong Luo, Chaojie Wang, Hongyu Han, He-Xun Huang, Wenlong Xu, Shuping Qu, Manman Wang, Zhichao Wang, and Yu-Juan Zhong
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0106 biological sciences ,0301 basic medicine ,Lutein ,Flesh color ,Cucurbita maxima ,Zeaxanthin ,Plant Science ,Orange (colour) ,01 natural sciences ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,SB1-1110 ,03 medical and health sciences ,chemistry.chemical_compound ,stomatognathic system ,Inbred strain ,β-carotene ,Carotenoid ,Ecology, Evolution, Behavior and Systematics ,chemistry.chemical_classification ,Ecology ,biology ,Renewable Energy, Sustainability and the Environment ,Flesh ,Plant culture ,food and beverages ,biology.organism_classification ,Horticulture ,030104 developmental biology ,chemistry ,010606 plant biology & botany ,Squash - Abstract
Mesocarp color is an important agronomic trait of Cucurbita maxima and is determined mainly by the contents and compositions of the carotenoids. The two inbred lines with significant differences in fruit flesh color were used in the study, the orange ‘312-1’ and white ‘98-2’. Changes in seven carotenoid contents and compositions in the flesh of fruit produced by inbred lines ‘312-1’ and ‘98-2’ were analyzed during fruit development. The expression of eight key carotenoid biosynthesis genes in the fruit flesh were investigated during five fruit development stage in two inbred lines. As the flesh color intensified, the orange flesh color of ‘312-1’ was determined mainly by the increased contents of lutein, β-carotene and zeaxanthin and the lack of carotenoid accumulation led to the formation of white flesh in ‘98-2’ fruit. The expression of the LCY-e and CHYb genes was significantly stronger in ‘312-1’ than in ‘98-2’, and their expression was strongly correlated with lutein and β-carotene contents during fruit development. This study provides a deep understanding of the molecular mechanism of carotenoid biosynthesis in fruit flesh and provides a basis for additional studies on the highly refined improvement of squash quality.
- Published
- 2021
17. Antioxidant and C5a-blocking strategy for hepatic ischemia–reperfusion injury repair
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Weibo Cai, Dong Wu, Jonathan W. Engle, Jiajia Hu, Shuping Qu, Kaelyn V Becker, Xiaobing Zhang, and Dalong Ni
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Antioxidant ,medicine.medical_treatment ,Pharmaceutical Science ,Medicine (miscellaneous) ,02 engineering and technology ,Pharmacology ,medicine.disease_cause ,01 natural sciences ,Applied Microbiology and Biotechnology ,Antioxidants ,Mice ,Ischemia ,Medicine ,chemistry.chemical_classification ,Cerium ,021001 nanoscience & nanotechnology ,Hepatic ischemia ,Nanomedicine ,Hepatic ischemia–reperfusion injury ,Nanoceria ,Liver ,Reperfusion Injury ,Cytokines ,Molecular Medicine ,Female ,Inflammation Mediators ,medicine.symptom ,0210 nano-technology ,Biotechnology ,C5a ,Aptamer ,Biomedical Engineering ,Complement C5a ,Bioengineering ,Inflammation ,010402 general chemistry ,Medical technology ,Animals ,Distribution (pharmacology) ,R855-855.5 ,Reactive oxygen species ,business.industry ,Research ,medicine.disease ,Molecular medicine ,0104 chemical sciences ,Disease Models, Animal ,Oxidative Stress ,Nanoantioxidants ,RAW 264.7 Cells ,chemistry ,Reactive Oxygen Species ,business ,Reperfusion injury ,Oxidative stress ,TP248.13-248.65 - Abstract
Background Nonspecific liver uptake of nanomaterials after intravenous injection has hindered nanomedicine for clinical translation. However, nanomaterials’ propensity for liver distribution might enable their use in hepatic ischemia–reperfusion injury (IRI) repair. During hepatic IRI, reactive oxygen species (ROS) are generated and the fifth component of complement (C5a) is activated. In addition, C5a is confirmed to exacerbate the vicious cycle of oxidative stress and inflammatory damage. For these reasons, we have investigated the development of nanomaterials with liver uptake to scavenge ROS and block C5a for hepatic IRI repair. Results To achieve this goal, a traditional nanoantioxidant of nanoceria was surface conjugated with the anti-C5a aptamers (Ceria@Apt) to scavenge the ROS and reduce C5a-mediated inflammation. High uptake of Ceria@Apt in the liver was confirmed by preclinical positron emission tomography (PET) imaging. The clinical symptoms of hepatic IRI were effectively alleviated by Ceria@Apt with ROS scavenging and C5a blocking in mice model. The released pro-inflammatory cytokines were significantly reduced, and subsequent inflammatory reaction involved in the liver was inhibited. Conclusions The synthesized Ceria@Apt has great potential of medical application in hepatic IRI repair, which could also be applied for other ischemic-related diseases. Graphic abstract
- Published
- 2021
18. Single cell transcriptional diversity and intercellular crosstalk of human liver cancer
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Yan Meng, Yan Sang, Jianping Liao, Qiudong Zhao, Shuping Qu, Rong Li, Jinghua Jiang, Meifeng Wang, Jiahong Wang, Dong Wu, Chun Cheng, and Lixin Wei
- Subjects
Cancer Research ,Cellular and Molecular Neuroscience ,Cancer-Associated Fibroblasts ,Immunology ,Liver Neoplasms ,Tumor Microenvironment ,Humans ,Cell Biology ,Single-Cell Analysis ,Signal Transduction - Abstract
Liver cancer arises from the evolutionary selection of the dynamic tumor microenvironment (TME), in which the tumor cell generally becomes more heterogeneous; however, the mechanisms of TME-mediated transcriptional diversity of liver cancer remain unclear. Here, we assess transcriptional diversity in 15 liver cancer patients by single-cell transcriptome analysis and observe transcriptional diversity of tumor cells is associated with stemness in liver cancer patients. Tumor-associated fibroblast (TAF), as a potential driving force behind the heterogeneity in tumor cells within and between tumors, was predicted to interact with high heterogeneous tumor cells via COL1A1-ITGA2. Moreover, COL1A1-mediated YAP-signaling activation might be the mechanistic link between TAF and tumor cells with increased transcriptional diversity. Strikingly, the levels of COL1A1, ITGA2, and YAP are associated with morphological heterogeneity and poor overall survival of liver cancer patients. Beyond providing a potential mechanistic link between the TME and heterogeneous tumor cells, this study establishes that collagen-stimulated YAP activation is associates with transcriptional diversity in tumor cells by upregulating stemness, providing a theoretical basis for individualized treatment targets.
- Published
- 2021
19. Identification and mapping of CpPM10.1, a major gene involved in powdery mildew (race 2 France of Podosphaera xanthi) resistance in zucchini (Cucurbita pepo L.)
- Author
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Yunli Wang, Man Li, Cong Qi, Yusong Luo, Feng Zhang, Zuyun Dai, and Shuping Qu
- Subjects
Germplasm ,Genetics ,Candidate gene ,biology ,Chromosome Mapping ,Locus (genetics) ,General Medicine ,biology.organism_classification ,Major gene ,Genetic analysis ,Chromosomes, Plant ,Cucurbita pepo ,Ascomycota ,Cucurbita ,Gene Expression Regulation, Plant ,Agronomy and Crop Science ,Powdery mildew ,Biotechnology ,Disease Resistance ,Plant Diseases ,Plant Proteins - Abstract
Powdery mildew resistance in zucchini is controlled by one major dominant locus, CpPM10.1. CpPM10.1 was fine mapped. The expression of candidate gene Cp4.1LG10g02780 in resistant individuals was significantly upregulated after inoculation with the powdery mildew. Powdery mildew (PM) is one of the most destructive fungal diseases, reducing the productivity of Cucurbita crops globally. PM influences the photosynthesis, growth and development of infected zucchini and seriously reduces fruit yield and quality. In the present study, the zucchini inbred line ‘X10′ had highly stable PM resistance, and the inbred line 'Jin234′ was highly susceptible to PM in the seedling stage and adult stages. Genetic analysis revealed that PM resistance in ‘X10′ is controlled by one major dominant locus. Based on the strategy of QTL-seq combined with linkage analysis and developed molecular markers, the major locus was found to be located in a 382.9-kb candidate region on chromosome 10; therefore, the major locus was named CpPM10.1. Using 1,400 F2 individuals derived from a cross between ‘X10′ and ‘JIN234′ and F2:3 offspring of the recombinants, the CpPM10.1 locus was defined in a region of approximately 20.9 kb that contained 5 coding genes. Among them, Cp4.1LG10g02780 contained a conserved domain (RPW8), which controls resistance to a broad range of PM pathogens. Cp4.1LG10g02780 also had nonsynonymous SNPs between the resistant ‘X10′ and susceptible ‘Jin234.′ Furthermore, the expression of Cp4.1LG10g02780 was strongly positively involved in PM resistance in the key period of inoculation. Further allelic diversity analysis in zucchini germplasm resources indicated that PM resistance was associated with two SNPs in the Cp4.1LG10g02780 RPW8 domain. This study not only provides highly stable PM resistance gene resources for cucurbit crops but also lays the foundation for the functional analysis of PM resistance and resistance breeding in zucchini.
- Published
- 2021
20. Multi‐omics analysis reveals a crosstalk between ferroptosis and peroxisomes on steatotic graft failure after liver transplantation
- Author
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Zhengtao Liu, Hai Zhu, Junsheng Zhao, Lu Yu, Shuping Que, Jun Xu, Lei Geng, Lin Zhou, Luca Valenti, and Shusen Zheng
- Subjects
liver transplantation ,macrosteatosis ,mechanism ,metabonomic ,prognosis ,transcriptomics ,Medicine - Abstract
Abstract To identify the mechanism underlying macrosteatosis (MaS)‐related graft failure (GF) in liver transplantation (LT) by multi‐omics network analysis. The transcriptome and metabolome were assayed in graft and recipient plasma in discovery (n = 68) and validation (n = 89) cohorts. Differentially expressed molecules were identified by MaS and GF status. Transcriptional regulatory networks were generated to explore the mechanism for MaS‐related inferior post‐transplant prognosis. The differentially expressed molecules associated with MaS and GF were enriched in ferroptosis and peroxisome‐related pathways. Core features of MaS‐related GF were presented on decreased transferrin and impaired anti‐oxidative capacity dependent upon dysregulation of transcription factors hepatocyte nuclear factor 4A (HNF4A) and hypoxia‐inducible factor 1A (HIF1A). Furthermore, miR‐362‐3p and miR‐299‐5p inhibited transferrin and HIF1A expression, respectively. Lower M2 macrophages but higher memory CD4 T cells were observed in MaS‐related GF cases. These results were validated in clinical specimens and cellular models. Systemic analysis of multi‐omics data depicted a panorama of biological pathways deregulated in MaS‐related GF. Transcriptional regulatory networks centered on transferrin and anti‐oxidant responses were associated with poor MaS graft quality, qualifying as potential targets to improve prognosis of patients after LT.
- Published
- 2024
- Full Text
- View/download PDF
21. Additional file 1 of Antioxidant and C5a-blocking strategy for hepatic ischemia–reperfusion injury repair
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Xiaobing Zhang, Jiajia Hu, Becker, Kaelyn V., Engle, Jonathan W., Dalong Ni, Weibo Cai, Wu, Dong, and Shuping Qu
- Subjects
Data_FILES - Abstract
Additional file 1. Additional figures.
- Published
- 2021
- Full Text
- View/download PDF
22. miR-361-3p Regulates Liver Tumor-initiating Cells Expansion and Chemo-resistance
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Yue Wu, Dong Wu, Shuping Qu, HengYu Li, Jian Zhai, and Xiaobing Zhang
- Subjects
0301 basic medicine ,Sorafenib ,Liver tumor ,SOX1 ,cisplatin ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,medicine ,Transcatheter arterial chemoembolization ,miR-361-3p ,Cisplatin ,Gene knockdown ,business.industry ,Tumor-initiating cells ,hepatocellular carcinoma ,medicine.disease ,digestive system diseases ,030104 developmental biology ,Oncology ,Hepatocellular carcinoma ,Cancer research ,030211 gastroenterology & hepatology ,sorafenib ,business ,medicine.drug ,Research Paper - Abstract
Background: Increasing evidence shows that liver tumor-initiating cells (T-ICs) closely associated with the progression, metastasis, recurrence and chemo-resistance of hepatocellular carcinoma (HCC). However, the underlying mechanism for the propagation of liver T-ICs remains unclear. The purpose of our study was to explore the role of miR-361-3p in the expansion of liver T-ICs and the potential molecular mechanism.Results: Flow cytometry analysis was performed to isolate CD24+, CD133+ or EpCAM+ cells from primary HCC cells or HCC cell lines. Real-time PCR was used to detect the expression of miR-361-3p in liver T-ICs. The impact of miR-361-3p on liver T-ICS expansion was investigated both in vivo and in vitro. The correlation between miR-361-3p expression and TACE (transcatheter arterial chemoembolization) or sorafenib benefits in HCC was evaluated in patient cohorts. miR-361-3p expression is upregulated in liver T-ICs. Knockdown of miR-361-3p impairs the self-renewal and tumorigenicity liver T-ICs. Conversely, forced miR-361-3p expression enhances the self-renewal and tumorigenicity liver T-ICs. Mechanistically, miR-361-3p directly targets SOX1 via binding its 3’-UTR in liver T-ICs. Moreover, miR-361-3p knockdown hepatoma cells are more sensitive to cisplatin or sorafenib treatment. Clinical cohort analysis demonstrates that miR-361-3p low HCC patients are benefited from TACE or sorafenib treatment. Conclusions: Our findings revealed the crucial role of the miR-361-3p in liver T-IC expansion and TACE or sorafenib response, rendering miR-361-3p an optimal target for the prevention and intervention in HCC.
- Published
- 2020
23. Fine mapping identified the gibberellin 2-oxidase gene CpDw leading to a dwarf phenotype in squash (Cucurbita pepo L.)
- Author
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Cong Qi, Ding Wenqi, Wenlong Xu, Chaojie Wang, Yunli Wang, Yusong Luo, and Shuping Qu
- Subjects
0106 biological sciences ,0301 basic medicine ,Crops, Agricultural ,Candidate gene ,Quantitative Trait Loci ,Dwarfism ,Locus (genetics) ,Plant Science ,Biology ,Genes, Plant ,01 natural sciences ,Genetic analysis ,Polymorphism, Single Nucleotide ,Mixed Function Oxygenases ,03 medical and health sciences ,Cucurbita pepo ,Cucurbita ,Gene Expression Regulation, Plant ,Genetics ,medicine ,Gene ,Plant Stems ,Bulked segregant analysis ,food and beverages ,Chromosome Mapping ,General Medicine ,medicine.disease ,biology.organism_classification ,030104 developmental biology ,Phenotype ,Agronomy and Crop Science ,010606 plant biology & botany - Abstract
Dwarfism is an important agronomic trait in pumpkin that can increase yield. In this study, the dwarf Cucurbita pepo L. line X10 exhibited significantly longitudinally shorter cell length in the stem than did the normal-vine line JIN234. The dwarf stature of X10 was recovered with exogenous gibberellin (GA3) application, suggesting that X10 might be sensitive to GA biosynthesis. Genetic analysis revealed that this dwarf trait is controlled by a single completely dominant locus: CpDw (Cucurbita pepo L. Dwarf). Using 1,300 F2 individuals derived from a cross between X10 and JIN234, we mapped the CpDw locus to a region of approximately 24.6 kb on chromosome 10 that contain 5 annotated genes. The high expression level of Cp4.1LG10g05910.1 and high GA2ox enzyme activity in X10 revealed that the GA 2-oxidase gene Cp4.1LG10g05910.1 is a candidate gene for CpDw. Alignment of the Cp4.1LG10g05910.1 gene revealed two nonsynonymous single nucleotide polymorphism (SNP) mutations in the two exons, as well as several SNPs and InDels in the important functional elements of promoter between parental lines. Further allelic diversity analysis of the Cucurbita spp. germplasm resources indicated that Cp4.1LG10g05910.1 may be involved in vine growth during the early developmental stage in C. pepo but not in C. maxima or C. moschata. This study provides an important theoretical basis for the genetic regulation of vine length and crop breeding in pumpkin.
- Published
- 2020
24. Weighted Gene Coexpression Network Analysis Reveals the Dynamic Transcriptome Regulation and Prognostic Biomarkers of Hepatocellular Carcinoma
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Hengwei Fan, Qiuyuan Shi, Jing Xu, Wanwan Yi, and Shuping Qu
- Subjects
Hepatocellular carcinoma ,lcsh:Evolution ,Computational biology ,Biology ,Transcriptome ,03 medical and health sciences ,0302 clinical medicine ,microRNA ,Genetics ,medicine ,lcsh:QH359-425 ,KEGG ,Gene ,Ecology, Evolution, Behavior and Systematics ,Survival analysis ,030304 developmental biology ,Original Research ,0303 health sciences ,Cell growth ,WGCNA ,medicine.disease ,Computer Science Applications ,differential expression analysis ,030220 oncology & carcinogenesis ,Biomarker (medicine) ,biomarker ,prognosis - Abstract
This study was aimed at revealing the dynamic regulation of mRNAs, long noncoding RNAs (lncRNAs), and microRNAs (miRNAs) in hepatocellular carcinoma (HCC) and to identify HCC biomarkers capable of predicting prognosis. Differentially expressed mRNAs (DEmRNAs), lncRNAs, and miRNAs were acquired by comparing expression profiles of HCC with normal samples, using an expression data set from The Cancer Genome Atlas. Altered biological functions and pathways in HCC were analyzed by subjecting DEmRNAs to Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis. Gene modules significantly associated with disease status were identified by weighted gene coexpression network analysis. An lncRNA-mRNA and an miRNA-mRNA coexpression network were constructed for genes in disease-related modules, followed by the identification of prognostic biomarkers using Kaplan-Meier survival analysis. Differential expression and association with the prognosis of 4 miRNAs were verified in independent data sets. A total of 1220 differentially expressed genes were identified between HCC and normal samples. Differentially expressed mRNAs were significantly enriched in functions and pathways related to “plasma membrane structure,” “sensory perception,” “metabolism,” and “cell proliferation.” Two disease-associated gene modules were identified. Among genes in lncRNA-mRNA and miRNA-mRNA coexpression networks, 9 DEmRNAs and 7 DEmiRNAs were identified to be potential prognostic biomarkers. MIMAT0000102, MIMAT0003882, and MIMAT0004677 were successfully validated in independent data sets. Our results may advance our understanding of molecular mechanisms underlying HCC. The biomarkers may contribute to diagnosis in future clinical practice.
- Published
- 2020
25. QTL mapping of domestication and diversifying selection related traits in round-fruited semi-wild Xishuangbanna cucumber (Cucumis sativus L. var. xishuangbannanesis)
- Author
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Yupeng Pan, Kristin R. Haider, Kailiang Bo, Yiqun Weng, Shuping Qu, and Meiling Gao
- Subjects
0106 biological sciences ,0301 basic medicine ,Genetic Linkage ,Quantitative Trait Loci ,Flowers ,Quantitative trait locus ,01 natural sciences ,Hypocotyl ,Domestication ,Crop ,03 medical and health sciences ,Botany ,Genetics ,Selection, Genetic ,photoperiodism ,biology ,Chromosome Mapping ,General Medicine ,biology.organism_classification ,Phenotype ,030104 developmental biology ,Seedling ,Genetic marker ,Fruit ,Cucumis sativus ,Agronomy and Crop Science ,Cucumis ,Microsatellite Repeats ,010606 plant biology & botany ,Biotechnology - Abstract
QTL analysis revealed 11 QTL underlying flowering time and fruit size variation in the semi-wild Xishuangbanna cucumber, of which, FT6.2 and FS5.2 played the most important roles in determining photoperiod-dependent flowering time and round-fruit shape, respectively. Flowering time and fruit size are two important traits in domestication and diversifying selection in cucumber, but their genetic basis is not well understood. Here we reported QTL mapping results on flowering time and fruit size with F2 and F2:3 segregating populations derived from the cross between WI7200, a small fruited, early flowering primitive cultivated cucumber and WI7167, a round-fruited, later flowering semi-wild Xishuangbanna (XIS) cucumber. A linkage map with 267 microsatellite marker loci was developed with 138 F2 plants. Phenotypic data of male and female flowering time, fruit length and diameter and three other traits (mature fruit weight and number, and seedling hypocotyl length) were collected in multiple environments. Three flowering time QTL, FT1.1, FT5.1 and FT6.2 were identified, in which FT6.2 played the most important role in conferring less photoperiod sensitive early flowering during domestication whereas FT1.1 seemed more influential in regulating flowering time within the cultivated cucumber. Eight consensus fruit size QTL distributed in 7 chromosomes were detected, each of which contributed to both longitudinal and radial growth in cucumber fruit development. Among them, FS5.2 on chromosome 5 exhibited the largest effect on the determination of round fruit shape that was characteristic of the WI7167 XIS cucumber. Possible roles of these flowering time and fruit size QTL in domestication of cucumber and crop evolution of the semi-wild XIS cucumber, as well as the genetic basis of round fruit shape in cucumber are discussed.
- Published
- 2017
26. Construction of a High-Density Genetic Map and Analysis of Seed-Related Traits Using Specific Length Amplified Fragment Sequencing for
- Author
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Yunli Wang, Chaojie Wang, Hongyu Han, Yusong Luo, Zhichao Wang, Chundong Yan, Wenlong Xu, and Shuping Qu
- Subjects
0106 biological sciences ,0301 basic medicine ,Cucurbita maxima ,Single-nucleotide polymorphism ,Plant Science ,lcsh:Plant culture ,Quantitative trait locus ,Biology ,01 natural sciences ,03 medical and health sciences ,Genetic linkage ,Cleaved amplified polymorphic sequence ,lcsh:SB1-1110 ,high-density genetic map ,Association mapping ,cleaved amplified polymorphic sequence ,Gene ,Original Research ,Genetics ,food and beverages ,biology.organism_classification ,seed-related traits ,030104 developmental biology ,Genetic distance ,specific length amplified fragment sequencing ,010606 plant biology & botany - Abstract
A high-density genetic map can provide a basis for quantitative trait locus (QTL) mapping and marker-assisted selection (MAS) based on single-nucleotide polymorphisms (SNPs). Specific length amplified fragment sequencing (SLAF-seq) has been successfully applied to construct high-density maps of many species but not Cucurbita maxima. In this study, 100 F2 individuals from two morphologically diverse parents, namely “2013-12” and “9-6”, were used to successfully construct a high-density genetic map of C. maxima using the SLAF-seq method. A total of 8,406 SLAF markers were used to construct a 3,376.87 cM genetic map for 20 linkage groups (LGs) with an average genetic distance of 0.47 cM. With these markers, total ten QTLs for seed-related traits were identified with composite interval mapping (CIM) method. The QTLs affecting seed width (SW), seed length (SL) and hundred-seed weight (HSW) explained a maximum 38.6%, 28.9% and 17.2% of the phenotypic variation and were detected in LG6, LG6 and LG17, respectively. To validate the locations determined by SLAF-seq, additional 150 F2 individuals were used for QTL mapping of SW and SL using cleaved amplified polymorphic sequence (CAPS) markers. The major QTL SL6-1 and SW6-1 locations identified by SLAF-seq overlapped the region identified in the F2 population analysis by the CAPS markers. Based on nonsynonymous SNPs and gene descriptions of the major SW- and SL-associated regions identified by SLAF-seq, we found that the VQ motif, E3 ubiquitin-protein ligase and F-box and Leucine Rich Repeat domains containing protein may play roles in SL and SW in C. maxima. This study reports the construction of the first high-density linkage map of C. maxima by using SNPs developed using SLAF-seq technology. The information obtained from the genetic map is a valuable tool for association mapping of important agronomic traits, map-based gene cloning and MAS-based breeding in C. maxima.
- Published
- 2019
27. HrpN
- Author
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Lijun, Sun, Xiaomeng, Wang, Shuping, Qu, Huimin, Liu, Zhenhua, Jia, and Hansong, Dong
- Abstract
HrpN
- Published
- 2019
28. Supplemental Material, Supporting_information_for_online_publication_only - A Pretreatment CT Model Predicts Survival Following Chemolipiodolization in Patients With Hepatocellular Carcinoma
- Author
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Yijun Zhang, Shuping Qu, Wanwan Yi, Zhai, Jian, Xiaobing Zhang, Lixin Wei, Lau, Wan Yee, Mengchao Wu, Shen, Feng, Hengwei Fan, and Wu, Dong
- Subjects
110320 Radiology and Organ Imaging ,FOS: Clinical medicine ,Biochemistry ,111299 Oncology and Carcinogenesis not elsewhere classified - Abstract
Supplemental Material, Supporting_information_for_online_publication_only for A Pretreatment CT Model Predicts Survival Following Chemolipiodolization in Patients With Hepatocellular Carcinoma by Yijun Zhang, Shuping Qu, Wanwan Yi, Jian Zhai, Xiaobing Zhang, Lixin Wei, Wan Yee Lau, Mengchao Wu, Feng Shen, Hengwei Fan and Dong Wu in Technology in Cancer Research & Treatment
- Published
- 2019
- Full Text
- View/download PDF
29. Rapid profiling of triacylglycerols for identifying authenticity of edible oils using supercritical fluid chromatography-quadruple time-of-flight mass spectrometry combined with chemometric tools
- Author
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Anqi Tu, Shuping Qu, and Zhenxia Du
- Subjects
Adulterant ,Chromatography ,010405 organic chemistry ,Chemistry ,General Chemical Engineering ,Glyceride ,010401 analytical chemistry ,General Engineering ,Mass spectrometry ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,Ingredient ,Score plot ,Principal component analysis ,Supercritical fluid chromatography ,Time-of-flight mass spectrometry - Abstract
Triacylglycerol (TAG) as the major ingredient of oil is a key indicator for the identification of oil adulteration due to its characteristic distributions in different oils. In this research, a high-throughput method for rapidly detecting acylglycerols in various edible oils was established by applying supercritical fluid chromatography (SFC) coupled with quadruple time-of-flight mass spectrometry (Q-TOF-MS), without any sample pretreatment procedure. The retention mechanism of TAGs on different columns were comprehensively evaluated including the previous work on an ODS column, as well as our study on HSS C18SB, BEH and BEH 2-EP columns. Distinctive retention of our method allows the separation of certain pairs of TAGs which remains difficult by traditional methods, and the analysis time is greatly reduced. SFC-MS data were subsequently analysed by principal component analysis (PCA) to make a clear classification between six different kinds of vegetable oils, showing its potential in differentiating the fakes quickly. Considering the high-price of olive oil, further quantification of olive oil adulteration was studied by estimating known and unknown compositions of blend oils according to the curves of areas of selected TAG markers versus adulterant concentrations. Good consistencies with the labeled content of olive oil in commercial blend oils demonstrated the reliability of the quantitative method, and these blend oils were differentiated distinctly from pure olive oils in the score plot of a PCA model. To the best of our knowledge, it was the first time that the retention of TAGs using different stationary phases in a SFC system was discussed, and that SFC was applied to quantify oil adulteration. This fast and effective method is of great advantage to authenticate edible oils.
- Published
- 2016
30. Soluble sugars accumulation and related gene expression during fruit development in Cucurbita maxima Duchesne
- Author
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Chaojie Wang, He-Xun Huang, Ding Wenqi, Wenlong Xu, Yunli Wang, Yu-Juan Zhong, Shuping Qu, Yusong Luo, Hongyu Han, and Manman Wang
- Subjects
0106 biological sciences ,0301 basic medicine ,Hexokinase ,Sucrose ,biology ,Horticulture ,biology.organism_classification ,01 natural sciences ,Fructokinase ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,Invertase ,chemistry ,biology.protein ,Sucrose synthase ,Sucrose-phosphate synthase ,Food science ,Gene ,Cucurbita maxima ,010606 plant biology & botany - Abstract
In this study, two pumpkin lines were used as experimental materials to study the accumulation of soluble sugars and the expression of related genes during the development of pumpkin fruits. There were differences in the soluble sugars contents and regulatory mechanisms between the two pumpkin lines. The glucose content of ‘312-1’ decreased rapidly in the early stage of fruit development, which may be due to the high expression of Sucrose invertase (INV) and Hexokinase (HK) at this stage. There were significant differences in sucrose contents between mature fruits of ‘312-1’ and ‘98-2’, and the high expression of Sucrose synthase (SUS) and Sucrose phosphate synthase (SPS) led to sucrose accumulation in these pumpkins. The relative expression of SPS in ‘312-1’ was higher than that in ‘98-2’, which led to more sucrose accumulation in the former. In contrast, the Fructokinase (FK), which is responsible for fructose metabolism, was differentially expressed at individual stages of fruit development. These results indicate that INV, SUS, SPS, HK and FK may play significant roles by contributing coordinately to sucrose biosynthesis during pumpkin fruit development. This study provides a deeper understanding of the molecular mechanism of sucrose biosynthesis and a basis for further studies on the improvement of fruit eating quality in pumpkin.
- Published
- 2020
31. Genetic diversity of pumpkin based on morphological and SSR markers
- Author
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Yunli, Wang, primary, Yangyang, Wang, additional, Wenlong, Xu, additional, Chaojie, Wang, additional, Chongshi, Cui, additional, and Shuping, Qu, additional
- Published
- 2019
- Full Text
- View/download PDF
32. miR-129 suppresses tumor cell growth and invasion by targeting PAK5 in hepatocellular carcinoma
- Author
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Zengqiang Qu, Shuping Qu, Xiaowei Li, Xiaoxia Chen, Jian Zhai, Jiaming Zhong, and Dong Wu
- Subjects
Male ,Carcinoma, Hepatocellular ,Biophysics ,Apoptosis ,Biology ,medicine.disease_cause ,Biochemistry ,Metastasis ,Downregulation and upregulation ,Cell Movement ,RNA interference ,microRNA ,Tumor Microenvironment ,medicine ,Humans ,Gene silencing ,Neoplasm Invasiveness ,RNA, Small Interfering ,Lung cancer ,3' Untranslated Regions ,Molecular Biology ,Cell Proliferation ,Neoplasm Staging ,Binding Sites ,Cell growth ,Liver Neoplasms ,Cell Biology ,Middle Aged ,medicine.disease ,digestive system diseases ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,p21-Activated Kinases ,Immunology ,Cancer research ,Blood Vessels ,Female ,Carcinogenesis ,Signal Transduction - Abstract
Emerging evidence suggests that microRNAs (miRNAs) play important roles in regulating HCC development and progression; however, the mechanisms by which their specific functions and mechanisms remained to be further explored. miR-129 has been reported in gastric cancers, lung cancer and colon cancer. In this study, we disclosed a new tumor suppresser function of miR-129 in HCC. We also found the downregulation of miR-129 occurred in nearly 3/4 of the tumors examined (56/76) compared with adjacent nontumorous tissues, which was more importantly, correlated to the advanced stage and vascular invasion. We then demonstrated that miR-129 overexpression attenuated HCC cells proliferation and invasion, inducing apoptosis in vitro. Moreover, we used miR-129 antagonist and found that anti-miR-129 promoted HCC cells malignant phenotypes. Mechanistically, our further investigations revealed that miR-129 suppressed cell proliferation and invasion by targeting the 3’-untranslated region of PAK5, as well as miR-129 silencing up-regulated PAK5 expression. Moreover, miR-129 expression was inversely correlated with PAK5 expression in 76 cases of HCC samples. RNA interference of PAK5 attenuated anti-miR-129 mediated cell proliferation and invasion in HCC cells. Taken together, these results demonstrated that miR-129 suppressed tumorigenesis and progression by directly targeting PAK5, defining miR-129 as a potential treatment target for HCC. - Highlights:more » • Decreased of miR-129 is found in HCC and associated with advanced stage and metastasis. • miR-129 suppresses proliferation and invasion of HCC cells. • miR-129 directly targets the 3′ UTR of PAK5 and diminishes PAK5 expression. • PAK5 is involved in miR-129 mediated suppression functions.« less
- Published
- 2015
33. Direct detection of free fatty acids in edible oils using supercritical fluid chromatography coupled with mass spectrometry
- Author
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Zhenxia Du, Shuping Qu, and Yun Zhang
- Subjects
Principal Component Analysis ,Spectrometry, Mass, Electrospray Ionization ,Electrospray ,Reproducibility ,Chromatography ,Column temperature ,Chemistry ,Temperature ,Reproducibility of Results ,Chromatography, Supercritical Fluid ,General Medicine ,Fatty Acids, Nonesterified ,Mass spectrometry ,Analytical Chemistry ,Food products ,Edible oil ,Supercritical fluid chromatography ,Food Technology ,Plant Oils ,Gradient elution ,lipids (amino acids, peptides, and proteins) ,Oils ,Food Science - Abstract
Determination of free fatty acids (FFAs) in food products is of enormous interest mainly because they are related to the quality and authenticity of the oils. In this study, supercritical fluid chromatography (SFC), followed by an electrospray ionisation triple-quadrupole mass spectrometry (ESI-MS), is shown to provide a novel method for the separation and detection of FFAs in edible oils without any pretreatment. Eight FFAs were separated on a HSS C18 SB column with gradient elution within 3 min. Effects of different columns, modifiers and column temperature changes were evaluated. The results indicated the feasibility of this method for the high-throughput determination of individual FFAs with satisfactory correlation coefficients (R(2)>0.994) and good reproducibility of RSD
- Published
- 2015
34. Sequence variations in the FAD2 gene in seeded pumpkins
- Author
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Shuping Qu, Wenlong Xu, Yu Ge, Y. Chang, and Cui Cs
- Subjects
Fatty Acid Desaturases ,Linoleic acid ,chemistry.chemical_compound ,Cucurbita pepo ,Cucurbita ,Genetics ,Humans ,Amino Acid Sequence ,Molecular Biology ,Peptide sequence ,Phylogeny ,chemistry.chemical_classification ,Sequence Homology, Amino Acid ,biology ,Genetic Variation ,food and beverages ,Fatty acid ,General Medicine ,biology.organism_classification ,Introns ,Amino acid ,chemistry ,Cucurbita moschata ,Seeds ,Fatty Acids, Unsaturated ,Cucurbita maxima - Abstract
Seeded pumpkins are important economic crops; the seeds contain various unsaturated fatty acids, such as oleic acid and linoleic acid, which are crucial for human and animal nutrition. The fatty acid desaturase-2 (FAD2) gene encodes delta-12 desaturase, which converts oleic acid to linoleic acid. However, little is known about sequence variations in FAD2 in seeded pumpkins. Twenty-seven FAD2 clones from 27 accessions of Cucurbita moschata, Cucurbita maxima, Cucurbita pepo, and Cucurbita ficifolia were obtained (totally 1152 bp; a single gene without introns). More than 90% nucleotide identities were detected among the 27 FAD2 clones. Nucleotide substitution, rather than nucleotide insertion and deletion, led to sequence polymorphism in the 27 FAD2 clones. Furthermore, the 27 FAD2 selected clones all encoded the FAD2 enzyme (delta-12 desaturase) with amino acid sequence identities from 91.7 to 100% for 384 amino acids. The same main-function domain between 47 and 329 amino acids was identified. The four species clustered separately based on differences in the sequences that were identified using the unweighted pair group method with arithmetic mean. Geographic origin and species were found to be closely related to sequence variation in FAD2.
- Published
- 2015
35. Short Communication Genetic linkage map of Cucurbita maxima with molecular and morphological markers
- Author
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Li X, Yu Ge, Shuping Qu, Yang Xx, and Cui Cs
- Subjects
Genetic Markers ,China ,Genetic Linkage ,Quantitative Trait Loci ,Locus (genetics) ,Breeding ,Quantitative trait locus ,Biology ,chemistry.chemical_compound ,Cucurbita ,Genetic linkage ,Genetics ,Molecular Biology ,Gene ,Crosses, Genetic ,Chromosome Mapping ,General Medicine ,biology.organism_classification ,Phenotype ,chemistry ,Genetic distance ,Maxima ,DNA ,Cucurbita maxima ,Microsatellite Repeats - Abstract
Cucurbita maxima is one of the most widely cultivated vegetables in China and exhibits distinct morphological characteristics. In this study, genetic linkage analysis with 57 simple-sequence repeats, 21 amplified fragment length polymorphisms, 3 random-amplified polymorphic DNA, and one morphological marker revealed 20 genetic linkage groups of C. maxima covering a genetic distance of 991.5 cM with an average of 12.1 cM between adjacent markers. Genetic linkage analysis identified the simple-sequence repeat marker 'PU078072' 5.9 cM away from the locus 'Rc', which controls rind color. The genetic map in the present study will be useful for better mapping, tagging, and cloning of quantitative trait loci/gene(s) affecting economically important traits and for breeding new varieties of C. maxima through marker-assisted selection.
- Published
- 2015
36. Hollow fiber liquid-phase microextraction combined with ultra-high performance liquid chromatography–tandem mass spectrometry for the simultaneous determination of naloxone, buprenorphine and norbuprenorphine in human plasma
- Author
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Shuping Qu, Zhenxia Du, and Wenjun Sun
- Subjects
Internal standard ,Liquid Phase Microextraction ,Electrospray ionization ,Clinical Biochemistry ,Analytical chemistry ,Mass spectrometry ,Biochemistry ,Analytical Chemistry ,chemistry.chemical_compound ,Limit of Detection ,Tandem Mass Spectrometry ,Liquid chromatography–mass spectrometry ,Humans ,Norbuprenorphine ,Quadrupole mass analyzer ,Chromatography, High Pressure Liquid ,Detection limit ,Chromatography ,Naloxone ,Chemistry ,Selected reaction monitoring ,Reproducibility of Results ,Cell Biology ,General Medicine ,Buprenorphine ,Linear Models - Abstract
A hollow fiber liquid phase microextraction (HF–LPME) combined with ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method was developed for the extraction and determination of naloxone (NLX), buprenorphine (BP) and its major metabolite norbuprenorphine (NBP) in human plasma. The optimum extraction conditions of HF–LPME were: the porous of polyvinylidene fluoride (PVDF) hollow fiber was full of component solvent (1-octanol/chloroform/toluene, 2/4/4), the pH of donor phase was 8.7, the extraction time was 30 min and stirring speed was 1000 revolutions per minute (rpm). The UHPLC–MS/MS method was performed with Waters ACQUITY UPLCTM BEH C18, 50 mm × 2.1 mm, 1.7 μm, using methanol–0.2%formic acid as mobile phase with a gradient elution at a flow rate of 0.25 mL/min. The target compounds were detected under a tandem quadrupole mass spectrometer in positive electrospray ionization (ESI) mode, then analyzed in multiple reaction monitoring (MRM) mode and the isotope internal standard method was used for quantification. The results showed that linearities were in the range of 0.1–25 ng/mL ( R > 0.996). The limits of detection (LOD) of BP/NBP/NLX were 0.05/0.05/0.025 ng/mL and the limits of quantitation (LOQ) of BP/NBP/NLX were 0.1/0.1/0.05 ng/mL, respectively. The spiked recoveries were in the range of 92.1–106.0% with relative standard deviation (RSD) values were less than 15%. This method was simple, inexpensive, sensitive and has been successfully used to quantify plasma samples from patients included in a clinical pharmacogenetic study.
- Published
- 2014
37. Morphological, Transcriptomic and Hormonal Characterization of Trimonoecious and Subandroecious Pumpkin (Cucurbita maxima) Suggests Important Roles of Ethylene in Sex Expression
- Author
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Bingxue Zou, Chongshi Cui, Chaojie Wang, Wenlong Xu, Chundong Yan, Yunli Wang, and Shuping Qu
- Subjects
0301 basic medicine ,Cucurbita maxima ,chemical treatment ,floral sex expression ,Stamen ,transcriptome sequencing ,Catalysis ,lcsh:Chemistry ,Inorganic Chemistry ,Transcriptome ,03 medical and health sciences ,0302 clinical medicine ,Gene expression ,Physical and Theoretical Chemistry ,KEGG ,lcsh:QH301-705.5 ,Molecular Biology ,Gene ,Spectroscopy ,biology ,Organic Chemistry ,food and beverages ,General Medicine ,biology.organism_classification ,Computer Science Applications ,Cell biology ,030104 developmental biology ,lcsh:Biology (General) ,lcsh:QD1-999 ,030220 oncology & carcinogenesis ,Shoot ,ethylene signal synthesis and transduction ,Signal transduction - Abstract
Sex expression is a complex process, and in-depth knowledge of its mechanism in pumpkin is important. In this study, young shoot apices at the one-true-leaf stage and 10-leaf stage in Cucurbita maxima trimonoecious line &lsquo, 2013&ndash, 12&rsquo, and subandroecious line &lsquo, 9&ndash, 6&rsquo, were collected as materials, and transcriptome sequencing was performed using an Illumina HiSeqTM 2000 System. 496 up-regulated genes and 375 down-regulated genes were identified between shoot apices containing mostly male flower buds and only female flower buds. Based on gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, the differentially expressed genes were mainly enriched in the ethylene and auxin synthesis and signal transduction pathways. In addition, shoot apices at the 4-leaf stage were treated with the ethylene-releasing agent 2-chloroethylphosphonic acid (Ethrel), aminoethoxyvinyl glycine (AVG), AgNO3 and indoleacetic acid (IAA). The number of female flowers up to node 20 on the main stem of &lsquo, increased significantly after Ethrel and IAA treatment and decreased significantly after AVG and AgNO3 treatment. The female flowers in &lsquo, showed slight changes after treatment with the exogenous chemicals. The expression of key genes in ethylene synthesis and signal transduction (CmaACS7, CmaACO1, CmaETR1 and CmaEIN3) was determined using quantitative RT-PCR, and the expression of these four genes was positively correlated with the number of female flowers in &lsquo, The variations in gene expression, especially that of CmaACS7, after chemical treatment were small in &lsquo, From stage 1 (S1) to stage 7 (S7) of flower development, the expression of CmaACS7 in the stamen was much lower than that in the ovary, stigma and style. These transcriptome data and chemical treatment results indicated that IAA might affect pumpkin sex expression by inducing CmaACS7 expression and indirectly affecting ethylene production, and the ethylene synthesis and signal transduction pathways play crucial roles in pumpkin flower sex expression. A possible reason for the differences in sex expression between pumpkin lines &lsquo, and &lsquo, was proposed based on the key gene expression. Overall, these transcriptome data and chemical treatment results suggest important roles for ethylene in pumpkin sex expression.
- Published
- 2019
38. A pretreatment CT model predicts survival following chemolipiodolization in patients with hepatocellular carcinoma
- Author
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Shuping Qu, Dong Wu, and Hengwei Fan
- Subjects
Cancer Research ,medicine.medical_specialty ,Oncology ,medicine.diagnostic_test ,business.industry ,Hepatocellular carcinoma ,medicine ,Prognostic model ,Computed tomography ,In patient ,Radiology ,business ,medicine.disease - Abstract
e14509 Background: This study aimed to establish a computed tomography-based prognostic model for patients with hepatocellular carcinoma treated with transarterial chemoembolization. Methods: Using prospectively collected data from 195 consecutive patients with hepatocellular carcinoma who underwent chemolipiodolization at the Eastern Hepatobiliary Surgery Hospital between 2013 and 2016, we established a prognostic model based on hepatocellular carcinoma enhancement patterns on computed tomography scans to predict the outcome of transarterial chemoembolization. Furthermore, a histopathology analysis was performed on 108 different patients undergoing resection between 2014 and 2016 to identify whether there was a correlation between enhancement pattern and microvessel density. Results: The prognostic model classified hepatocellular carcinoma into 3 types: Type I, which reached peak enhancement during the arterial phase and had a high mean microvessel density (101.5 vessels/0.74 mm2); Type II, which reached peak enhancement during the portal venous or delayed phase and had an intermediate microvessel density (53.6 vessels/0.74 mm2); and Type III, in which the tumor was insignificantly enhanced and had a low microvessel density (21.1 vessels/0.74 mm2). For type I, II, and III hepatocellular carcinoma, the post-transarterial chemoembolization 1-year tumor complete necrosis rates were 13.7%, 36.5%, and 0%, respectively (p < 0.001) and the 3-year overall survival rates were 14.1%, 38.6%, and 0%, respectively (p < 0 .001). Conclusions: Our results indicate that hepatocellular carcinoma type is an independent predictor of complete necrosis and overall survival.
- Published
- 2019
39. A Pretreatment CT Model Predicts Survival Following Chemolipiodolization in Patients With Hepatocellular Carcinoma
- Author
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Xiaobing Zhang, Yijun Zhang, Dong Wu, Meng-Chao Wu, Wan Yee Lau, Jian Zhai, Shuping Qu, Feng Shen, Wanwan Yi, Lixin Wei, and Hengwei Fan
- Subjects
Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,Computed tomography ,transarterial chemoembolization ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,In patient ,Chemoembolization, Therapeutic ,Aged ,Neoplasm Staging ,Neovascularization, Pathologic ,medicine.diagnostic_test ,business.industry ,Liver Neoplasms ,tumor vascularity ,Disease Management ,computed tomography ,hepatocellular carcinoma ,Middle Aged ,Prognosis ,medicine.disease ,Treatment Outcome ,Oncology ,030220 oncology & carcinogenesis ,Tumor vascularity ,Hepatocellular carcinoma ,Prognostic model ,Female ,Original Article ,030211 gastroenterology & hepatology ,Radiology ,Neoplasm Grading ,Tomography, X-Ray Computed ,business - Abstract
Purpose: To establish a computed tomography–based prognostic model for patients with hepatocellular carcinoma treated with transarterial chemoembolization. Materials and Methods: Using prospectively collected data from 195 consecutive patients with hepatocellular carcinoma who underwent chemolipiodolization at the Eastern Hepatobiliary Surgery Hospital between 2013 and 2016, we established a prognostic model based on hepatocellular carcinoma enhancement patterns on computed tomography scans to predict the outcome of transarterial chemoembolization. Furthermore, a histopathology analysis was performed on 108 different patients undergoing resection between 2014 and 2016 to identify whether there was a correlation between enhancement pattern and microvessel density. Results: The prognostic model classified hepatocellular carcinoma into 3 types: type I, which reached peak enhancement during the arterial phase and had a high mean microvessel density (101.5 vessels/0.74 mm2); type II, which reached peak enhancement during the portal venous or delayed phase and had an intermediate microvessel density (53.6 vessels/0.74 mm2); and type III, in which the tumor was insignificantly enhanced and had a low microvessel density (21.1 vessels/0.74 mm2). For type I, II, and III hepatocellular carcinoma, the post-transarterial chemoembolization 1-year tumor complete necrosis rates were 13.7%, 36.5%, and 0%, respectively ( P < .001), and the 3-year overall survival rates were 14.1%, 38.6%, and 0%, respectively ( P < .001). Conclusion: Our results indicate that hepatocellular carcinoma type is an independent predictor of complete necrosis and overall survival
- Published
- 2019
40. Morphological and molecular diversity in a germplasm collection of seed pumpkin
- Author
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Xue Li, Yu Ge, Dong-Jie Wang, Chong-Shi Cui, Shuping Qu, Chao Liu, and Xiao-Xia Yang
- Subjects
Genetic divergence ,Germplasm ,Crop ,Cucurbita pepo ,Genetic diversity ,Botany ,UPGMA ,food and beverages ,Cultivar ,Horticulture ,Biology ,biology.organism_classification ,Cucurbita maxima - Abstract
Seed pumpkin is an important economic crop grown in Heilongjiang Province, China that comprises distinct cultivars of Cucurbita maxima and C. pepo. However, little is known about the genetic diversity of seed pumpkin. Morphological and molecular characterizations were used to investigate the genetic diversity and genetic relationships within a germplasm collection that included 38 Chinese and 28 Russian seed cultivars of C. maxima, and 10 Chinese seed genotypes of C. pepo. The most variable morphological characters were all usually associated with the fruit. Thirty-five SSR markers yielded a total of 85 amplified DNA fragments, of which 85.89% were polymorphic, indicating a high degree of genetic diversity. The information provided by the SSR markers was agreed with the morphological diversity. Principal components analysis (PCA), principal coordinates analysis (PCO), and UPGMA clustering clearly distinguished the accessions based on species and geographic regions for both morphological and molecular characterizations. This indicated that genetic divergence has occurred between germplasm from China and that from Russia. On the other hand, geographical location also appeared to have affected genetic diversity due to adaptation of the plants to the different environments. This finding suggested that molecular information on genetic relationships in combination with morphological characteristics would serve to (1) improve the classification system of seed pumpkin cultivars in cultivation for germplasm conservation, (2) enhance the economic value of seed pumpkin.
- Published
- 2013
41. HrpNEa Induces Chinese Cabbage Resistance to Bacterial Soft Rot by Inhibiting the Bacterial Attachment to Root Surfaces
- Author
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Lijun Sun, Huimin Liu, Xiaomeng Wang, Hansong Dong, Zhenhua Jia, and Shuping Qu
- Subjects
Pectobacterium ,biology ,food and beverages ,Plant Science ,Erwinia ,Plant disease resistance ,biology.organism_classification ,Microbiology ,Bacterial soft rot ,Plant species ,Agronomy and Crop Science ,Pathogen ,Systemic acquired resistance ,Bacteria - Abstract
HrpNEa is a harpin protein produced by the bacterial plant pathogen Erwinia amylovora. When applied to aerial parts of plants, the protein induces systemic acquired resistance in a variety of plant species. Here, we report that treating Chinese cabbage roots with HrpNEa induces resistance of the plant to Pectobacterium carotovora subsp. carotovora, the pathogen that invades roots and causes bacterial soft rot in cruciferous plants. Treating Chinese cabbage roots with HrpNEa significantly decreased severities of soft rot symptoms on the plant. The root treatment decreased the number of P. carotovora subsp. carotovora cells attached to root surfaces and inhibited the ability of P. carotovora subsp. carotovora to produce quorum-sensing signals, which regulate pathogenicity in a bacterial population-dependent manner. The inhibitory effects of HrpNEa on the root attachment and quorum-sensing signals accompanied the induced expression of several defense response genes. These results suggest that HrpNEa induces Chinese cabbage resistance to bacterial soft rot by inhibiting the bacterial attachment to root surfaces.
- Published
- 2010
42. ESTABLISHMENT OF CUCURBITA MAXIMA GENETIC TRANSFORMATION SYSTEM BY AGROBACTERIUM-MEDIATED METHOD
- Author
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Shuping Qu, Zhugang Li, Chongshi Cui, Xi Zhao, and Hongbing Fu
- Subjects
Acetosyringone ,Cefotaxime ,food.ingredient ,biology ,Agrobacterium ,food and beverages ,Kanamycin ,Horticulture ,biology.organism_classification ,Microbiology ,chemistry.chemical_compound ,food ,chemistry ,medicine ,Cucurbita ,Cucurbita maxima ,Cotyledon ,medicine.drug ,Transformation efficiency - Abstract
This research using Jinhuiyihao (Cucurbita maxima Duch) cotyledon studied many influencing factors by agrobacterium-mediated method. They are pre-culture time, infection time and concentration of acetosyringone (AS), co-infection time and the concentration of carbenicillin (Carb), cefotaxime (Cef) and kanamycine (Kan). Then a suitable genetic transformation system was established. The results indicated that no pre-culturation, infection for 30 min, acetosyringone (AS) with a concentration of 100mg/L and co-infection for 5 days would get high transformation efficiency. The optimal bactericide is Cef, and the medium with Cefotaxime 500 mg/L, kanamycin 100 mg/L. Eventually, culturing the resistance shoots in MS, the result of Southern blot and PCR showed that the kanamycin-resistant foreign gene was integrated into the genome of Cucurbita.
- Published
- 2010
43. A Fragment of the Xanthomonas oryzae pv. oryzicola Harpin HpaGXooc Reduces Disease and Increases Yield of Rice in Extensive Grower Plantings
- Author
-
Shujian Zhang, Xiuyan Ren, Jun Qian, Lingxian Wang, Shuping Qu, Feng Sun, Juying Long, Tingquan Wu, Chunling Zhang, Qian Yin, Zhongkai Zhang, Zaiquan Cheng, Shao-Song Zhang, Xiaojing Wu, Lei Chen, Hansong Dong, Steven V. Beer, and Marshall L. Hayes
- Subjects
China ,Xanthomonas ,Oryza sativa ,Geography ,biology ,food and beverages ,Oryza ,Plant Science ,Plant disease resistance ,biology.organism_classification ,Immunity, Innate ,Xanthomonas oryzae ,Agronomy ,Plant defense against herbivory ,Poaceae ,Cultivar ,Agronomy and Crop Science ,Bacteria ,Bacterial Outer Membrane Proteins ,Plant Diseases ,Pseudomonadaceae - Abstract
Harpins of phytopathogenic bacteria stimulate defense and plant growth in many types of plants, conferring disease resistance and enhanced yield. In a previous study, we characterized nine fragments of the harpin protein HpaGXooc from Xanthomonas oryzae pv. oryzicola for plant defense elicitation and plant growth stimulation activity relative to the intact protein. In plants grown under controlled conditions, the fragment HpaG10-42 was more active in both regards than HpaGXooc. Here, we demonstrate that the activity of HpaG10-42 in rice under field conditions significantly exceeds that of HpaGXooc, stimulating resistance to three important diseases and increasing grain yield. We carried out tests in 672 experimental plots with nine cultivars of rice planted at three locations. Application protocols were optimized by testing variations in application rate, frequency, and timing with respect to rice growth stage. Of the concentrations (24, 24, 12, and 6 μg/ml), and number and timing of applications (at one to four different stages of growth) tested, HpaG10-42 at 6 μg/ml applied to plants once at nursery seedling stage and three times in the field was most effective. Bacterial blight, rice blast, and sheath blight were reduced 61.6 and 56.4, 93.6 and 76.0, and 93.2 and 55.0% in indica and japonica cultivars, respectively, relative to controls. Grain yields were 22 to 27% greater. These results are similar to results obtained with typical local management practices, including use of chemicals, to decrease disease severities and increase yield in rice. Our results demonstrate that the HpaG10-42 protein fragment can be used effectively to control diseases and increase yield of this staple food crop.
- Published
- 2008
44. The ABI2-dependent abscisic acid signalling controls HrpN-induced drought tolerance in Arabidopsis
- Author
-
Hansong Dong, Jianling Peng, Hong-Ping Dong, Zhilong Bao, Zhen Yao, Xiaojing Guo, Guangyong Chen, Shuping Qu, and Haiqin Yu
- Subjects
Mutant ,Drought tolerance ,Adaptation, Biological ,Arabidopsis ,Plant Science ,chemistry.chemical_compound ,Gene Expression Regulation, Plant ,Botany ,Phosphoprotein Phosphatases ,Genetics ,Pseudomonas syringae ,Arabidopsis thaliana ,Proline ,Abscisic acid ,Dehydration ,biology ,Effector ,Gene Expression Profiling ,fungi ,food and beverages ,biology.organism_classification ,Cell biology ,Phenotype ,chemistry ,Mutation ,Abscisic Acid ,Bacterial Outer Membrane Proteins ,Signal Transduction - Abstract
HrpN, a protein produced by the plant pathogenic bacterium Erwinia amylovora, has been shown to stimulate plant growth and resistance to pathogens and insects. Here we report that HrpN activates abscisic acid (ABA) signalling to induce drought tolerance (DT) in Arabidopsis thaliana L. plants grown with water stress. Spraying wild-type plants with HrpN-promoted stomatal closure decreased leaf transpiration rate, increased moisture and proline levels in leaves, and alleviated extents of damage to cell membranes and plant drought symptoms caused by water deficiency. In plants treated with HrpN, ABA levels increased; expression of several ABA-signalling regulatory genes and the important effector gene rd29B was induced or enhanced. Induced expression of rd29B, promotion of stomatal closure, and reduction in drought severity were observed in the abi1-1 mutant, which has a defect in the phosphatase ABI1, after HrpN was applied. In contrast, HrpN failed to induce these responses in the abi2-1 mutant, which is impaired in the phosphatase ABI2. Inhibiting wild-type plants to synthesize ABA eliminated the role of HrpN in promoting stomatal closure and reducing drought severity. Moreover, resistance to Pseudomonas syringae developed in abi2-1 as in wild-type plants following treatment with HrpN. Thus, an ABI2-dependent ABA signalling pathway is responsible for the induction of DT but does not affect pathogen defence under the circumstances of this study.
- Published
- 2004
45. Graft-to-recipient weight ratio exerts nonlinear effects on prognosis by interacting with donor liver macrosteatosis
- Author
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Zhengtao Liu, Jingting Lyu, Xiang Li, Lu Yu, Shuping Que, Jun Xu, Lei Geng, and Shusen Zheng
- Subjects
GWRWR ,prognosis ,graft failure ,liver transplantation ,macrosteatosis ,Surgery ,RD1-811 - Abstract
AimTo investigate the interactions between the graft-to-recipient weight ratio (GWRWR) and other risk factors responsible for inferior allograft outcomes.MethodsA total of 362 patients who received liver transplantation (LT) were enrolled. Indicators such as graft/recipient weight and other prognostic factors were collected. Comparisons of indicators and survival analysis were performed in groups categorized by the GWRWR. Interactions of large-for-size grafts (LFSGs) with graft macrosteatosis (MaS) were evaluated in terms of relative excess risk caused by interaction (RERI) and attributable proportion (AP). Cytoscape visualized the role of LFSGs in the risk profile for poor prognosis.ResultsBased on the GWRWR, LT cases can be categorized into three subgroups, standard (1%–2.5%), optimal (2.5%–3.0%), and inferior prognosis (>3.0%). Survival analysis confirmed clear separations in cases categorized by the above-defined limits on the GWRWR (P 3.0%) caused inferior outcomes, while grafts sized within (2.5%–3.0%) had optimal post-transplant prognosis. MaS increased the risk of poor prognosis by exerting positive synergistic effects on LFSGs.
- Published
- 2023
- Full Text
- View/download PDF
46. Risk factors and management for early and late intrahepatic recurrence of solitary hepatocellular carcinoma after curative resection
- Author
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Feng Shen, Jiahua Zhou, Norbert Hüser, Jun Li, Shuping Qu, Pinghua Yang, Dong Wu, Xinwei Yang, Baohua Zhang, Jue Yang, Kui Wang, Yong Xia, Zhenlin Yan, and Zhangjun Cheng
- Subjects
Curative resection ,Adult ,Male ,medicine.medical_specialty ,China ,Carcinoma, Hepatocellular ,Adolescent ,Hepatic resection ,Gastroenterology ,Risk Assessment ,Young Adult ,Risk Factors ,Internal medicine ,Late Recurrence ,medicine ,Hepatectomy ,Humans ,Aged ,Retrospective Studies ,Hepatology ,business.industry ,Incidence ,Liver Neoplasms ,Original Articles ,Middle Aged ,medicine.disease ,Prognosis ,digestive system diseases ,Survival Rate ,Hepatocellular carcinoma ,Female ,Neoplasm Recurrence, Local ,business ,Follow-Up Studies - Abstract
BackgroundIntrahepatic recurrence is a significant problem for patients who have undergone a hepatic resection for hepatocellular carcinoma (HCC). The objective of the present study was to identify risk factors and evaluate the management of early and late recurrence of solitary HCC after curative resection.MethodsIncluded in this study were 816 patients with solitary HCC who underwent a curative partial hepatectomy. Intrahepatic recurrence in these patients was followed up retrospectively. Prognosis and therapy for the recurrence were investigated and analysed.ResultsEarly and late intrahepatic recurrence occurred in 423 patients and 199 patients, respectively. Multivariate analysis showed that a tumour diameter >5 cm, the absence of a tumour capsule and the presence of microvascular invasion were correlated with early recurrence, whereas cirrhosis and alphafetal protein >400 μg/l were independent risk factors contributing to late recurrence. The 5year survival of HCC patients with early recurrence was significantly lower than that of patients with late recurrence. Further curative treatment for intrahepatic recurrence offered a 5year overall survival of 56.0%, which was better than alternative management.ConclusionEarly and late recurrences of solitary HCC after curative resection are associated with different predictive factors. The time to recurrence and further curative treatment after recurrence were the best predictors of survival post recurrence.
- Published
- 2014
47. Detection of seven pesticides in cucumbers using hollow fibre-based liquid-phase microextraction and ultra-high pressure liquid chromatography coupled to tandem mass spectrometry
- Author
-
Zhenxia Du, Jianfeng Wang, Wenlian Yu, and Shuping Qu
- Subjects
Calibration curve ,Liquid Phase Microextraction ,Analytical chemistry ,Phenylcarbamates ,Sodium Chloride ,Tandem mass spectrometry ,Biochemistry ,Analytical Chemistry ,Limit of Detection ,Tandem Mass Spectrometry ,Desorption ,Acetamides ,Sample preparation ,Chromatography, High Pressure Liquid ,Detection limit ,Chromatography ,Chemistry ,Triazines ,Organic Chemistry ,Pesticide Residues ,Reproducibility of Results ,General Medicine ,Triazoles ,Solvent ,Linear Models ,Salting out ,Chloroform ,Cucumis sativus ,Enrichment factor - Abstract
A liquid-phase microextraction (LPME) methodology based on the use of porous polyvinylidene fluoride (PVDF) hollow fibres was developed for extracting seven pesticides from cucumbers. The seven pesticides include propoxur, carbofuran, atrazine, cyanatryn, metolachlor, prometryn and tebuconazole. The PVDF hollow fibre provides higher extraction efficiency due to its higher porosity and better solvent compatibility. A new desorption methodology was developed since some pesticides were absorbed by the wall pore of the PVDF. Ultra-high pressure liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS) was used for pesticide analysis. In order to obtain high recoveries and enrichment factors of the analytes, several parameters such as method of sealing, acceptor phase (organic solvents), stirring speed, extraction time, salting out effect, desorption mode and time were optimized. A fast, simple method for closing fibre ends was practiced by using mechanical crimping. Pesticides were extracted from the sample to the organic solvent and then desorbed in a mixture of methanol:water (1:1 v/v) prior to chromatographic analysis. Limits of detection (LOD) for the multi-reaction-monitoring (MRM) mode of the method varies from 0.01 to 0.31 μg/kg with optimized sample preparation. Calibration curves are linear with R 2 ≥ 0.991. Enrichment factor of the hollow fibre LPME ranges from 100 to 147. Matrix effect has been considered and is in the range of 76–122%. The relative recoveries from cucumber samples are between 63% and 119% with the relative standard deviation (RSD, n = 6) lower than 20%.
- Published
- 2012
48. Photocatalytic degradation of hexazinone and its determination in water via UPLC-MS/MS
- Author
-
Ruifen Xu, Haojie Zhang, Mei Mei, Shuping Qu, Yun Chen, and Zhenxia Du
- Subjects
Detection limit ,Environmental Engineering ,Chromatography ,Chemistry ,Photochemistry ,Triazines ,Health, Toxicology and Mutagenesis ,Pollution ,High-performance liquid chromatography ,Hexazinone ,Catalysis ,chemistry.chemical_compound ,Adsorption ,Microscopy, Electron, Transmission ,Tandem Mass Spectrometry ,Carbon dioxide ,Photocatalysis ,Urea ,Environmental Chemistry ,Degradation (geology) ,Waste Management and Disposal ,Chromatography, High Pressure Liquid ,Water Pollutants, Chemical - Abstract
Degradation of hexazinone has been investigated by means of photocatalysis of mixed-phase crystal nano-TiO2. Influences of adsorption, amount of nano-TiO2, pH and irradiation time on the photocatalytic process are studied. Results show that hexazinone is totally degraded within 40 min of irradiation under pH neutral conditions. This compares favorably with Degussa P25 TiO2 when conducted under the same experimental conditions. Preliminary photocatalytic kinetic information for hexazinone degradation is proposed. First order kinetics is obtained for the adsorption and photocatalytic degradation reactions, which fit the Langmuir–Hinshelwood model. A rapid, sensitive and accurate UPLC–MS/MS technique is developed and utilized to determine the level of hexazinone in water in support of the degradation kinetics study. The results indicate a limit of detection (LOD) at 0.05 μg/l and the recoveries between 90.2 and 98.5% with relative standard deviations (RSD) lower than 12%. A LC–MS/MS technique is used to trace the degradation process. Complete degradation is achieved into final products including nontoxic water, carbon dioxide and urea. A probable pathway for the total photocatalytic degradation of hexazinone is proposed.
- Published
- 2011
49. Identification of specific fragments of HpaG Xooc, a harpin from Xanthomonas oryzae pv. oryzicola, that induce disease resistance and enhance growth in plants
- Author
-
Lei Chen, Qian Yin, Marshall L. Hayes, Hansong Dong, Chunling Zhang, Shuping Qu, Steven V. Beer, Xiaojing Wu, Feng Sun, Jun Qian, Juying Long, and Tingquan Wu
- Subjects
Oryza sativa ,Xanthomonas ,biology ,Nicotiana tabacum ,food and beverages ,Mutagenesis (molecular biology technique) ,Plant Development ,Oryza ,Plant Science ,Plant disease resistance ,Plants ,biology.organism_classification ,Polymerase Chain Reaction ,Immunity, Innate ,Microbiology ,Magnaporthe ,Xanthomonas oryzae ,Mutation ,Tobacco ,Magnaporthe grisea ,Agronomy and Crop Science ,Gene ,Bacterial leaf streak ,Bacterial Outer Membrane Proteins ,Plant Diseases - Abstract
Harpin proteins from gram-negative plant-pathogenic bacteria can stimulate hypersensitive cell death (HCD) and pathogen defense as well as enhance growth in plants. Two of these diverse activities clearly are beneficial and may depend on particular functional regions of the proteins. Identification of beneficial and deleterious regions might facilitate the beneficial use of harpin-related proteins on crops without causing negative effects like cell death. Here, we report the identification and testing of nine functional fragments of HpaGXooc, a 137-amino-acid harpin protein from Xanthomonas oryzae pv. oryzicola, the pathogen that causes bacterial leaf streak of rice. Polymerase chain reaction-based mutagenesis generated nine proteinaceous fragments of HpaGXooc; these caused different responses following their application to Nicotiana tabacum (tobacco) and Oryza sativa (rice). Fragment HpaG62-137, which spans the indicated amino acid residues of the HpaG, induced more intense HCD; in contrast, HpaG10-42 did not cause evident cell death in tobacco. However, both fragments stimulated stronger defense responses and enhanced more growth in rice than the full-length parent protein, HpaGXooc. Of the nine fragments, the parent protein and one deletion mutant of HpaGXooc tested, HpaG10-42, stimulated higher levels of rice growth and resulted in greater levels of resistance to X. oryzae pv. oryzae and Magnaporthe grisea. These pathogens cause bacterial leaf blight and rice blast, respectively, the two most important diseases of rice world-wide. HpaG10-42 was more active than HpaGXooc in inducing expression of several genes that regulate rice defense and growth processes and activating certain signaling pathways, which may explain the greater beneficial effects observed from treatment with that fragment. Overall, our results suggest that HpaG10-42 holds promise for practical agricultural use to induce disease resistance and enhance growth of rice.
- Published
- 2008
50. Systematic Assessment of Safety Threshold for Donor Age in Cadaveric Liver Transplantation
- Author
-
Wenchao Wang, Zhengtao Liu, Junjie Qian, Jun Xu, Shuping Que, Li Zhuang, Lei Geng, Lin Zhou, and Shusen Zheng
- Subjects
liver transplantation ,donor age ,mortality ,outcomes ,dose-response analysis ,Medicine (General) ,R5-920 - Abstract
Background: Donor age affects allograft quality and the prognosis of recipients after liver transplantation (LT). Clinicians have assessed the quality of grafts from older donors based on their appearance and texture, with no reliable quantitative evidence. Our study aimed to assess the quantitative impact of donor age on post-transplant outcomes and its safety threshold for LT, based on the published literature.Methods: Relevant studies were retrieved from the Embase, PubMed, and ISI Web of Science databases. Pooled dichotomous relative risks (RRs) were calculated using metan. Continuous RRs were calculated using a two-stage random-effects model.Results: Eleven studies including 30,691 LT cases were included for further analysis. For categorical comparison, the RR of death within the first post-transplant year was significantly higher among patients who received grafts from older donors. Similarly, the RR of graft failure (GF) was increased within the 3 years after transplantation. For continuous comparison, advanced donor age affected transplant outcomes in a linear manner (P > 0.05). A 10-year increment in donor age was associated with RRs 1.10, 1.12, 1.15, 1.10, and 1.08 for 90-day, 180-day, 1-year, 3-year, and 5-year patient mortality and 1.08, 1.06, 1.10, 1.11, and 1.12, for 90-day, 180-day, 1-year, 2-year, and 3-year GF, respectively (all P < 0.05). A spline model showed that transplants using grafts from donors 0.05). The risk of GF was increased in subgroups with fewer LT cases, longer cold ischemic time, fewer male donors, and recipients with viral hepatitis (P < 0.05).Conclusion: Donor age might affect post-LT outcomes in a dose-dependent manner. The safety threshold for donor age in terms of GF should be lowered to 43 years as an early warning for the guarantee of satisfactory outcomes. Clinicians should weigh the benefits against the risks carefully for patients receiving grafts from older donors. Further studies are warranted to investigate the mechanisms responsible for the relationship between donor age and graft quality.
- Published
- 2021
- Full Text
- View/download PDF
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