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20–30% of patients with nasopharyngeal carcinoma (NPC) develop distant metastasis or recurrence leading to poor survival, of which the underlying key molecular events have yet to be addressed. Here alternative splicing events in 85 NPC samples are profiled using transcriptome analysis and it is revealed that the long isoform of GOLIM4 (‐L) with exon‐7 is highly expressed in NPC and associated with poor prognosis. Lines of evidence demonstrate the pro‐tumorigenic function of GOLIM4‐L in NPC cells. It is further revealed that RBFOX2 binds to a GGAA motif in exon‐7 and promotes its inclusion forming GOLIM4‐L. RBFOX2 knockdown suppresses the tumorigenesis of NPC cells, phenocopying GOLIM4‐L knockdown, which is significantly rescued by GOLIM4‐L overexpression. High expression of RBFOX2 is correlated with the exon‐7 inclusion of GOLIM4 in NPC biopsies and associated with worse prognosis. It is observed that RBFOX2 and GOLIM4 can influence vesicle‐mediated transport through maintaining the organization of Golgi apparatus. Finally, it is revealed that RAB26 interacts with GOLIM4 and mediates its tumorigenic potentials in NPC cells. Taken together, the findings provide insights into how alternative splicing contributes to NPC development, by highlighting a functional link between GOLIM4‐L and its splicing regulator RBFOX2 activating vesicle‐mediated transport involving RAB26., Alternative splicing events are profiled in nasopharyngeal carcinoma (NPC) using transcriptome analysis, and a high expression of the long isoform of GOLIM4 containing exon‐7 (GOLIM4‐L) to be associated with poor survival of NPC is identified. A functional link between GOLIM4‐L and its splicing regulator RBFOX2 is also revealed, which activates vesicle‐mediated transport through maintaining Golgi complex integrity involving RAB26.

RNA binding motif proteins (RBMs) have been widely implicated in the tumorigenesis of multiple human cancers but scarcely studied in nasopharyngeal carcinoma (NPC). Here, we compare the mRNA levels of 29 RBMs between 87 NPC and 10 control samples. We find that RBM47 is frequently upregulated in NPC specimens, and its high expression is associated with the poor prognosis of patients with NPC. Biological experiments show that RBM47 plays an oncogenic role in NPC cells. Mechanically, RBM47 binds to the promoter and regulates the transcription of BCAT1, and its overexpression partially rescues the inhibitory effects of RBM47-knockdown on NPC cells. Moreover, transcriptome analysis reveals that RBM47 regulates alternative splicing of pre-mRNA, including those cancer-related, to a large extent in NPC cells. Furthermore, RBM47 binds to hnRNPM and cooperatively regulates multiple splicing events in NPC cells. In addition, we find that knockdown of hnRNPM inhibits proliferation and migration of NPC cells. Our study, taken together, shows that RBM47 promotes the progression of NPC through multiple pathways, acting as a transcriptional factor and a modulator of alternative splicing in cooperation with hnRNPM. Our study also highlights that RBM47 and hnRNPM could be prognostic factors and potential therapeutic targets for NPC.

Dysfunctional immune response in the COVID-19 patients is a recurrent theme impacting symptoms and mortality, yet the detailed understanding of pertinent immune cells is not complete. We applied single-cell RNA sequencing to 284 samples from 196 COVID-19 patients and controls and created a comprehensive immune landscape with 1.46 million cells. The large dataset enabled us to identify that different peripheral immune subtype changes were associated with distinct clinical features including age, sex, severity, and disease stages of COVID-19. SARS-CoV-2 RNAs were found in diverse epithelial and immune cell types, accompanied by dramatic transcriptomic changes within viral positive cells. Systemic up-regulation of S100A8/A9, mainly by megakaryocytes and monocytes in the peripheral blood, may contribute to the cytokine storms frequently observed in severe patients. Our data provide a rich resource for understanding the pathogenesis and developing effective therapeutic strategies for COVID-19., Highlights Detailed COVID-19 immune landscape depicted by integrated 1.46 million single cells Peripheral immune subtypes differentially associated with distinct clinical features SARS-CoV-2 RNAs are present in diverse epithelial and immune cells Megakaryocytes and monocyte subsets may contribute to cytokine storms, Analysis of immune landscape in the lung and peripheral blood of COVID patients across different regions in China at the single cell level documents the presence of viral RNAs in diverse cell types and highlights the potential contribution of megakaryocytes and monocyte subsets to cytokine storms.

Demand for cold chain logistics enterprises is growing to accommodate the growth of China’s economy and the enhancement of Chinese people’s living standard. However, the country’s cold chain logistics industry remains backward, thus making it a major task that how to raise the enterprise performance. Firstly, the study has chosen 5 primary indicators and 19 secondary indicators pertaining to cold chain logistics enterprises to set up its performance evaluation framework. Secondly, based on the merits and demerits of the BPNN model structure and it's calculation method, the study identifies the network layers, stimulant functions, and study perimeters of the BPNN model, and constructs the BPNN model for the performance evaluation of cold chain logistics enterprises. Finally, the study uses MATLAB7.0 software to calculate the BPNN model, and utilizes sim functions to simulate and analyze the feasibility of the BPNN method for cold chain logistics enterprise performance evaluation. The study firstly introduces BPNN technology into the performance indicators evaluation of cold chain logistics enterprises.

The authors have adopted acupuncture at Jľngjiājľ ( ) combined with nape cluster needling in treatment of 100 patients with cervicogenic headache. Nape cluster needling was: Xianaohu ( ) (located in the median depression under occipital bone), Fēngfŭ ( GV 16) and Yamen ( GV 15) were selected longitudinally; horizontally, the part from GV 16 to Wangŭ ( GB 12) was divided into six equal sections, one section was an acupoint, and there were 12 acupoints in total at the left and right sides. Bilateral Jľngjiājľ ( ) points on the second vertebra to the seventh vertebra were selected. The acupuncture was conducted once a day, five days were considered as one course of treatment, and two days were free from treatment between two courses. Four courses of treatment were needed. All the patients were cured clinically. It can be seen that acupuncture at Jľngjiājľ combined with nape cluster needling in treatment of cervicogenic headache has sound effect.

A modified quick, easy, cheap, effective, rugged, and safe sample preparation method combined with gas chromatography–negative chemical ionization–mass spectrometry (GC–NCI/MS) has been developed for the determination of 7 dinitroaniline herbicide residues in complex matrices (garlic, olive oil, scallion, leek and chili). Dinitroaniline residues were extracted with hexane-saturated acetonitrile. After the cleanup with the dispersive solid-phase extraction, the extract was analyzed by GC–NCI/MS in selected ion monitoring mode. Two isotope internal standards (trifluralin-d 14 and pendimethalin-d 5) were employed for quantification. Compared with electron ionization (EI) mode, the superiorities of NCI in sensitivity and selectivity were investigated. Limits of the detection of 7 dinitroanilines were in the range of 0.014–0.096 μg kg−1, and there were no interfering peaks (unlike in EI) in the complex matrices. Recoveries of 7 dinitroanilines in five matrices at three spiked levels (10, 20 and 40 μg kg−1) ranged from 61 to 126 % and the relative standard deviations were all below 12 %.

During polymer flooding, certain amount of polymer would be lost. Polymer retention causes sweep volume expanding on one side, it also causes polymer loss on the other. Therefore, it is a very important topic to study the influencing factors of polymer retention. There are many factors affecting polymer retention process. This paper mainly studied the influence from dynamic factors such as polymer solution concentration, injection rate, injection time, injected pv number. This paper investigated the influence of these factors on polymer retention process, and optimized these factors to minimize polymer loss in reservoir.

Limited studies exist related to the siro-spun spinning of flax ( Linum usitatissimum L.) and cotton (Gossypium hirsutum) blends. The purpose of this study was to determine the influence of various siro-spun processing parameters, including twist factor, space between two rovings and specification of traveler, on yarn qualities, such as yarn hairiness, tenacity and evenness. Results show that both specification of traveler and space between two rovings significantly influenced yarn hairiness and evenness, and heavier traveler and greater space led to decreased hairiness, but bad evenness. Twist factor influenced yarn evenness highly significantly, and smaller twist factor led to worse yarn evenness. Results also indicate the optimum levels of siro-spun process parameters as follows: twist factor αm = 150, space between two rovings 8 mm, traveler Fo 5/0. In relation to the general ring-spinning yarn, the siro-spun spinning yarns had decreased hairiness, little increased tenacity and similar evenness.

In this paper, an improved online stator current analysis method is proposed for detecting the rotor broken bar fault of three-phase squirrel-cage asynchronous motors. With the rapid development of the advanced digital filtering technique (such as ZOOM-FFT) and the rotor slot harmonics (RSH) techniques for slip measurement, it is possible to accurately estimate a motor’s slip rate from the precise measurements of the harmonic components of a rotor and the power supply frequency. This enables us to find the characteristic spectrum of a bar-broken rotor from the stator current spectrum. We proposed an online algorithm to detect the motor broken bar fault by localising and checking the existence of the fault-related characteristic spectrum. The proposed method overcomes the drawback of traditional current spectral analysis approaches. In particular, this paper addresses the problem that the side lobe spectral components are covered by the fundamental frequency and noises. This method has been validated in our experiment with a 30 kW three-phase induction motor. The experiment results show that the proposed method is able to detect small broken rotor bar fault with good application perspective.