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21 results on '"Shu, Qingbo"'

5. Assay design for unambiguous identification and quantification of circulating pathogen-derived peptide biomarkers

Author

Shu, Qingbo, primary, Liu, Shan, additional, Alonzi, Tonino, additional, LaCourse, Sylvia M., additional, Singh, Dhiraj Kumar, additional, Bao, Duran, additional, Wamalwa, Dalton, additional, Jiang, Li, additional, Lyon, Christopher J., additional, John-Stewart, Grace, additional, Kaushal, Deepak, additional, Goletti, Delia, additional, and Hu, Tony, additional

Published
2022
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16. Comparative proteomics reveals abnormal binding of ATGL and dysferlin on lipid droplets from pressure overload-induced dysfunctional rat hearts

Author

Li, Linghai, primary, Zhang, Huina, additional, Wang, Weiyi, additional, Hong, Yun, additional, Wang, Jifeng, additional, Zhang, Shuyan, additional, Xu, Shimeng, additional, Shu, Qingbo, additional, Li, Juanfen, additional, Yang, Fuquan, additional, Zheng, Min, additional, Qian, Zongjie, additional, and Liu, Pingsheng, additional

Published
2016
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17. Effect of superoxide dismutase and diethyldi-thicarbamate on the induction of squamous cell carcinoma of lung withmethyl-cholanthren in rats

Author

Zhang Yongxing, Zou Zuyu, Diao Luming, Liu Ming-qiu, Xia Dong, Huang Chunni, Zhou Jie, Chen Xiangao, Liu Xiao-yi, Tang Hua, Tian Hongsheng, Yu Lunyin, Wu Tianpeng, and Shu Qingbo

Subjects
Cancer Research, Pathology, medicine.medical_specialty, Lung, integumentary system, biology, business.industry, fungi, Cancer, medicine.disease, Epithelium, Atypical hyperplasia, Superoxide dismutase, chemistry.chemical_compound, medicine.anatomical_structure, Oncology, chemistry, Methylcholanthrene, medicine, biology.protein, Basal cell, Lung cancer, business
Abstract

The squamous cell carcinoma of lung was induced with methylcholanthrene (MCA) in iodized oil in wistar rts. During the development of the cancer, the animals were given superoxide dismutase (SOD) or its inhibitor diethyldio-carbamate (DDC). In DDC group, 3 out of 50 rats developed cancer and 4 developed atypical hyperplasia of hronchial epithelium within 35 to 40 days. In SOD group, no cancer developed in all of the 52 rats, and only one had atypical hyperplasia in the lungs. Only one of 42 control rats had cancer and 2 rats had atypical hyperplasia of bronchial epithelium. The difference in cancer frequency between groups DDC and SOD was significant (P

Published
1995

20. Cerebrospinal Fluid Protein Markers Indicate Neuro-Damage in SARS-CoV-2-Infected Nonhuman Primates

Author

Maity, Sudipa, Mayer, Meredith G., Shu, Qingbo, Linh, Hellmers, Bao, Duran, Blair, Robert V., He, Yanlin, Lyon, Christopher J., Hu, Tony Y., Fischer, Tracy, and Fan, Jia

Abstract

Neurologic manifestations are among the most frequently reported complications of COVID-19. However, given the paucity of tissue samples and the highly infectious nature of the etiologic agent of COVID-19, we have limited information to understand the neuropathogenesis of COVID-19. Therefore, to better understand the impact of COVID-19 on the brain, we used mass-spectrometry–based proteomics with a data-independent acquisition mode to investigate cerebrospinal fluid (CSF) proteins collected from two different nonhuman primates, Rhesus Macaque and African Green Monkeys, for the neurologic effects of the infection. These monkeys exhibited minimal to mild pulmonary pathology but moderate to severe central nervous system (CNS) pathology. Our results indicated that CSF proteome changes after infection resolution corresponded with bronchial virus abundance during early infection and revealed substantial differences between the infected nonhuman primates and their age-matched uninfected controls, suggesting these differences could reflect altered secretion of CNS factors in response to SARS-CoV-2-induced neuropathology. We also observed the infected animals exhibited highly scattered data distributions compared to their corresponding controls indicating the heterogeneity of the CSF proteome change and the host response to the viral infection. Dysregulated CSF proteins were preferentially enriched in functional pathways associated with progressive neurodegenerative disorders, hemostasis, and innate immune responses that could influence neuroinflammatory responses following COVID-19. Mapping these dysregulated proteins to the Human Brain Protein Atlas found that they tended to be enriched in brain regions that exhibit more frequent injury following COVID-19. It, therefore, appears reasonable to speculate that such CSF protein changes could serve as signatures for neurologic injury, identify important regulatory pathways in this process, and potentially reveal therapeutic targets to prevent or attenuate the development of neurologic injuries following COVID-19.

Published
2023
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21. Profiling and relative quantitation of phosphoinositides by multiple precursor ion scanning based on phosphate methylation and isotopic labeling.

Author

Cai T, Shu Q, Hou J, Liu P, Niu L, Guo X, Liu CC, and Yang F

Subjects
Animals, Cattle, Chromatography, Liquid, Diazomethane chemistry, Humans, Isotope Labeling, Male, Methylation, Lipids analysis, Phosphates chemistry, Phosphatidylinositols analysis, Prostatic Neoplasms metabolism, Spectrometry, Mass, Secondary Ion methods
Abstract

Phosphoinositides, the phosphorylated derivatives of phosphatidylinositol (PtdIns), are key regulators of many fundamental biological processes, including cell growth, proliferation, and motility. Here, we present a novel method for rapid, sensitive, and simultaneous profiling of phosphatidylinositol trisphosphate (PtdInsP3), phosphatidylinositol bisphosphate (PtdInsP2), and phosphatidylinositol phosphate (PtdInsP) of different fatty acid compositions. This method is based on a technique called "charged diacylglycerol fragment ion-specific multiple precursor ion scanning" (DAG(+)-specific MPIS), coupled with prior phosphate methylation. Using DAG(+)-specific MPIS, we were able to identify 32 PtdIns, 28 PtdInsP, 30 PtdInsP2, and 3 PtdInsP3 molecular species from bovine brain extracts or prostatic cancer cell lines in an efficient and time-saving manner. Our analysis revealed a large range of fatty acyl compositions in phosphoinositides not obtained previously from mammalian samples. We also developed a method that involves isotopic labeling of endogenous phosphoinositides with deuterated diazomethane (CD2N2) for quantitation of phosphoinositides. CD2N2 was generated in situ through acid-catalyzed H/D exchange and methanolysis of trimethylsilyl diazomethane (TMS-diazomethane). Phosphoinositides, extracted from a PC3 prostatic cancer cell line, were labeled either with CH2N2 or CD2N2 and mixed in known proportions for DAG(+)-specific MPIS-based mass spectrometry (MS) analysis. The results indicate that isotopic labeling is capable of providing accurate quantitation of PtdInsP3, PtdInsP2, and PtdInsP with adequate linearity as well as high reproducibility with an average coefficient variation of 18.9%. More importantly, this new methods excluded the need for multiple phosphoinositide internal standards. DAG(+)-specific MPIS and isotopic labeling based MS analysis of phosphoinositides offers unique advantages over existing approaches and presents a powerful tool for research of phosphoinositide metabolism.

Published
2015
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