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Background: Acute myocardial infarction (AMI) occurred in the heart, which underwent long‐term ischemia, and was mainly caused by hypoxia. Recently, studies have uncovered the participation of long noncoding RNAs (lncRNAs) in the pathogenesis of heart disease. Here, we planned to probe the role and molecular basis of ANRIL in hypoxia‐induced H9c2 cell injury. Methods: Trypan blue exclusion assay and Transwell and flow cytometry assays were conducted to assess hypoxia‐induced injury by determining the viability, migration, invasion, and apoptosis of H9c2 cells in different conditions, respectively. Gene expressions were evaluated by quantitative real‐time polymerase chain reaction or western blot analysis as needed. RNA immunoprecipitation and luciferase reporter assays were applied to confirm the associations among genes. Results: ANRIL expression was dramatically enhanced in hypoxia‐injured H9c2 cells, and silencing ANRIL aggravated hypoxia‐induced H9c2 cell injury. ANRIL positively regulated sirtuin 1 (SIRT1) expression via competitively binding with miR‐7‐5p. Moreover, inhibition of miR‐7‐5p counteracted ANRIL depletion‐exacerbated injury in hypoxic H9c2 cells, meanwhile, forced SIRT1 expression attenuated the injury‐promoting effect of miR‐7‐5p upregulation on hypoxic H9c2 cells. Conclusion: Our findings disclosed that ANRIL plays a protective part in hypoxia‐induced H9c2 cell injury via modulating the miR‐7‐5p/SIRT1 axis, suggesting the great potential of ANRIL as a protective target for AMI. [ABSTRACT FROM AUTHOR]

The aim of the current study was to investigate the effects and the underlying mechanisms of troxerutin on myocardial cell apoptosis during ischemia‐reperfusion (I/R) injury. Hypoxia/reoxygenation (H/R) model in neonatal rat cardiomyocytes, and I/R model in rats, were established following troxerutin preconditioning. The quantitative real‐time polymerase chain reaction analysis was performed to examine the messenger RNA miR‐146a‐5p expression in cardiomyocytes and myocardial tissues. Hemodynamic parameters and serum creatine kinase, lactate dehydrogenase, tumor necrosis factor‐α, and interleukin‐10 were evaluated. Infarct size was examined by 2,3,5‐triphenyltetrazolium chloride staining. Besides, myocardial apoptosis was detected by terminal deoxynucleotidyl transferase (dUTP) nick end labeling (TUNEL) assay. Western blot analysis was performed to determine the protein levels of caspase‐3, Bax, and Bcl‐2. The results showed that, troxerutin decreased rat cardiomyocyte apoptosis during H/R injury. Furthermore, the antiapoptotic effect of troxerutin against I/R injury was mediated by miR‐146a‐5p downregulation. In vivo experiments suggested that troxerutin alleviated myocardial I/R injury in rats via inhibition of miR‐146a‐5p. In conclusion, troxerutin exerted cardioprotective effects during I/R injury by downregulating miR‐146a‐5p. In this study, we examined for the first time, the underlying mechanism through which troxerutin exert neuroprotective effect via regulating specific microRNAs. We concluded that, troxerutin alleviated myocardial ischemia‐reperfusion injury by downregulating miR‐146a‐5p. [ABSTRACT FROM AUTHOR]

Background: Coronary artery bypass grafting (CABG) is an important therapeutic measure for CHD patients. The patients who score more than 12 EuroSCORE points cannot achieve good results because of their low cardiac output and delicate left ventricular function. Therefore, use of an intra-aortic balloon pump (IABP) is essential for coronary surgical patients in the peri-operative period. At present, there is no unified standard about when to insert an IABP. This study aimed to compare the short-term clinical outcomes of the IABP inserted in the preoperative condition with its use in the emergency condition for extremely high-risk patients., Material and Methods: IABP support time, respirator support time, and ICU stay time were significantly shorter (all p<0.05) in the preoperative IABP group compared to the emergency IABP group, and the rates of low cardiac output syndrome (LCOS), acute myocardial infarction, and acute kidney injury in the preoperative group were also significantly lower in the preoperative IABP group (all p<0.05). There were no significant differences in IABP-related complications and the mortality (p=0.106) between two groups., Results: Compared to the emergency IABP group, the IABP support time, respirator support time and ICU stay time were significantly lower in the preoperative IABP group (all p<0.05), and the rates of LCOS, acute myocardial infarction, and acute kidney injury in the preoperative group were also significantly lower (all p<0.05). There were no significant differences in IABP-related complications and the mortality (p=0.106) between the 2 groups., Conclusions: For high-risk patients with CABG, preoperative IABP insertion is a safe and effective measure.

Unlabelled: The authors model diabetes in rats, and inject the rats with Klotho gene. Then the levels of serum lipoprotein are tested, measuring the thickness of coronary artery intima and the ratio of intima and media to see whether Klotho gene has protective effect of coronary artery in diabetes rats., Methods: Extracting the Klotho gene from kidney tissue in the normal SD rats amplifies the target gene by PCR and uses adenovirus as carrier. Then SD rats were randomly divided into model group, control group and the treatment group for diabetes modeling. Transferred the Klotho gene into treatment group and blank adenovirus into control group by the experimenters. Nothing was done for model group. Rats were killed after a successful modeling in the twelfth week, then tested blood low-density lipoprotein, high density lipoprotein, and the coronary artery intima-media thickness. After doing these, intima-media thickness ratio was tested., Results: The high density lipoprotein is 0.67 ± 0.06 mmol/L in treatment group, 0.48 ± 0.10 mmol/L in control group, 0.47 ± 0.10 mmol/L in model group. The treatment group, control group and model group respectively two independent sample tests. There is statistical significance between treatment group and the other group p<0.01 in treatment group. The low density lipoprotein is 0.44 ± 0.08 mmol/L in treatment group, 0.45 ± 0.10 mmol/L in control group, 0.44 ± 0.05 mmol/L in model group. Respectively two independent sample test show that there is no statistical significance between treatment group and the other group (p>0.05). Intima thickness is 1.74 ± 0.05 μm in treatment group, 2.23 ± 0.06 μm in control group, 2.15 ± 0.05 μm in model group. There is statistical significance between treatment group and the other group (p<0.01). The ratio of intima and media is 0.237 ± 0.097 in treatment group, 0.308 ± 0.023 in control group, 0.316 ± 0.037 in model group, and t test, there is statistical significance between treatment group and the other group (p<0.01)., Conclusion: There is protective effect on coronary after Klotho gene was transferred into diabetic rats.