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With the internet speed and bandwidth growth along with the rise of smartphones, the content consumption behaviour has changed drastically in the late 2010s and early 2020s. With the ease of internet bandwidth availability, the OTT platforms like Netflix, Prime Video, Hotstar have eclipsed the cinemas and television networks. The purpose of this research is to understand the behaviour of movie and content watchers with respect to their preference of OTT over cinemas or vice-versa and predict how will it impact the future trends in the entertainment industry. In simple words, which platform has got a better future – OTT or Cinemas?

Collective cell migration is crucial to maintain epithelium integrity during developmental and repair processes. It requires a tight regulation of mechanical coordination between neighboring cells. This coordination embraces different features including mechanical self-propulsion of individual cells within cellular colonies and large-scale force transmission through cell–cell junctions. This review discusses how the plasticity of biomechanical interactions at cell–cell contacts could help cellular systems to perform coordinated motions and adapt to the properties of the external environment.

This paper studied about the role of different independent factors on supply chain responsiveness. Here, we considered factors such as joint decision making, information quality and performance risk as three independent variables and supply chain responsiveness as a dependent variable. We tried to survey different supply chain professionals working in different sectors to have an unbiased image in our study. After applying Pearson corelation in our study, we understood the significance of our independent variables on supply chain responsiveness. The positive significance shows the importance of these factors while working on our dependent variable. All three relationships are showcasing either positive or strong corelation. Finally, we could infer that there are a lot more factors that we can take in to consideration for calculating the impact on supply chain responsiveness in future studies. This will definitely strengthen the case and will help in practical explanation.

Cell manipulation in droplets has emerged as one of the great successes of microfluidic technologies, with the development of single-cell screening. However, the droplet format has also served to go beyond single-cell studies, namely by considering the interactions between different cells or between cells and their physical or chemical environment. These studies pose specific challenges linked to the need for long-term culture of adherent cells or the diverse types of measurements associated with complex biological phenomena. Here we review the emergence of droplet microfluidic methods for culturing cells and studying their interactions. We begin by characterizing the quantitative aspects that determine the ability to encapsulate cells, transport molecules, and provide sufficient nutrients within the droplets. This is followed by an evaluation of the biological constraints such as the control of the biochemical environment and promoting the anchorage of adherent cells. This first part ends with a description of measurement methods that have been developed. The second part of the manuscript focuses on applications of these technologies for cancer studies, immunology, and stem cells while paying special attention to the biological relevance of the cellular assays and providing guidelines on improving this relevance.

Cytotoxic T cells are important components of natural anti-tumor immunity and are harnessed in tumor immunotherapies. Immune responses to tumors and immune therapy outcomes largely vary among individuals, but very few studies examine the contribution of intrinsic behavior of the T cells to this heterogeneity. Here we show the development of a microfluidic-based in vitro method to track the outcome of antigen-specific T cell activity on many individual cancer spheroids simultaneously at high spatiotemporal resolution, which we call Multiscale Immuno-Oncology on-Chip System (MIOCS). By combining parallel measurements of T cell behaviors and tumor fates with probabilistic modeling, we establish that the first recruited T cells initiate a positive feedback loop to accelerate further recruitment to the spheroid. We also provide evidence that cooperation between T cells on the spheroid during the killing phase facilitates tumor destruction. Thus, we propose that both T cell accumulation and killing function rely on collective behaviors rather than simply reflecting the sum of individual T cell activities, and the possibility to track many replicates of immune cell-tumor interactions with the level of detail our system provides may contribute to our understanding of immune response heterogeneity.

T cell-based tumor immunotherapies such as CAR T cells or immune checkpoint inhibitors harness the cytotoxic potential of T cells to promote tumor regression. However, patient response to immunotherapy remains heterogeneous, highlighting the need to better understand the rules governing a successful T cell attack. Here, we develop a microfluidic-based method to track the outcome of T cell activity on many individual cancer spheroids simultaneously, with a high spatiotemporal resolution. By combining these parallel measurements of T cell behaviors and tumor fate with probabilistic modeling, we establish that the first recruited T cells initiate a positive feedback loop leading to an accelerated effector accumulation on the spheroid. We also provide evidence that cooperation between T cells on the spheroid during the killing phase facilitates tumor destruction. We propose that tumor destruction does not simply reflect the sum of individual T cell activities but relies instead on collective behaviors promoting both T cell accumulation and function. The possibility to track many replicates of immune-tumor interactions with such a level of detail should help delineate the mechanisms and efficacy of various immunotherapeutic strategies.

The directed migration of cell collectives is essential in various physiological processes, such as epiboly, intestinal epithelial turnover, and convergent extension during morphogenesis as well as during pathological events like wound healing and cancer metastasis 1,2 . Collective cell migration leads to the emergence of coordinated movements over multiple cells. Our current understanding emphasizes that these movements are mainly driven by large-scale transmission of signals through adherens junctions 3,4 . In this study, we show that collective movements of epithelial cells can be triggered by polarity signals at the single cell level through the establishment of coordinated lamellipodial protrusions. We designed a minimalistic model system to generate one-dimensional epithelial trains confined in ring shaped patterns that recapitulate rotational movements observed in vitro in cellular monolayers and in vivo in genitalia or follicular cell rotation 5–7 . Using our system, we demonstrated that cells follow coordinated rotational movements after the establishment of directed Rac1-dependent polarity over the entire monolayer. Our experimental and numerical approaches show that the maintenance of coordinated migration requires the acquisition of a front-back polarity within each single cell but does not require the maintenance of cell-cell junctions. Taken together, these unexpected findings demonstrate that collective cell dynamics in closed environments as observed in multiple in vitro and in vivo situations 5,6,8,9 can arise from single cell behavior through a sustained memory of cell polarity.

The directed migration of cell collectives is essential in various physiological processes, such as epiboly, intestinal epithelial turnover, and convergent extension during morphogenesis as well as during pathological events like wound healing and cancer metastasis1,2. Collective cell migration leads to the emergence of coordinated movements over multiple cells. Our current understanding emphasizes that these movements are mainly driven by large-scale transmission of signals through adherens junctions3,4. In this study, we show that collective movements of epithelial cells can be triggered by polarity signals at the single cell level through the establishment of coordinated lamellipodial protrusions. We designed a minimalistic model system to generate one-dimensional epithelial trains confined in ring shaped patterns that recapitulate rotational movements observed in vitro in cellular monolayers and in vivo in genitalia or follicular cell rotation5–7. Using our system, we demonstrated that cells follow coordinated rotational movements after the establishment of directed Rac1-dependent polarity over the entire monolayer. Our experimental and numerical approaches show that the maintenance of coordinated migration requires the acquisition of a front-back polarity within each single cell but does not require the maintenance of cell-cell junctions. Taken together, these unexpected findings demonstrate that collective cell dynamics in closed environments as observed in multiple in vitro and in vivo situations5,6,8,9 can arise from single cell behavior through a sustained memory of cell polarity.

Locating and securing an Alzheimer's patient who is outdoors and in wandering is crucial to an Alzheimer patient safety. Although advances in geo tracking and mobile technology have made locating patients instantly possible, reaching them while in wandering state may take time. However, a social network of caregivers may help shorten the time that it takes to reach and secure a wandering AD patient. This study proposes a new type of intervention based on novel mobile application architecture to form and direct a social support network of caregivers for locating and securing wandering patients as soon as possible. System employs, aside from the conventional tracking mechanism, a wandering detection mechanism, both of which operates through a tracking device installed a Subscriber Identity Module for Global System for Mobile Communications Network GSM . Keywords Alzheimer Disease AD , Global System for Mobile Communication GSM , Global Positioning System GPS , Mild Cognitive Impairment MCI , Electronic Geo Tracking EGT , Short Message Service SMS , Radio Frequency Identification RFID , Secure Digital Card SD Card , Digital to Analog Converter DAC , Active Radio Frequency Identification Localization System ARFIDLS , General Packet Radio Service GPRS , Real time Locating Systems RTLS . Haroon Dar | Shreyansh Jain | Jyoti Shukla | V. M. Sardeshmukh "Alzheimer Patient Tracking and Alert System" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-3 , April 2018, URL: https://www.ijtsrd.com/papers/ijtsrd11218.pdf

Collective cell migration (CCM) can be described as a large scale coordinated movement of cells that are in close proximity with each other. It is a phenomenon that is observed not only in physiological processes such as that found in embryogenesis and wound healing but also in pathophysiological processes such as cancer metastasis. Some of the factors influencing this concerted process include cell–cell adhesion, cell–substrate interaction and mechanical cues such as geometrical constraints among others. Here, we review recent research work done to investigate CCM of adherent cells. We highlight the classical example of an in vitro cell monolayer to illustrate our current understanding of the different mechanobiological mechanisms involved as these cells respond to the mechanical cues present in their environment. It is hoped that such understanding may potentially lead to better therapeutic strategies for diseases such as cancer and for tissue engineering and repair.

To establish feasibility and superiority of whey based nutritional beverage using established qualitative and quantitative tests for milk proteins and inclusion of cereal based additives. Additionally ensure that whey produced in processing milk; an important source of nutrition; is utilized to its maximum potential. Commercial milk processing recycles whey to extract soluble nutrients, hence whey discarded by commercial milk processing plants is of little use. On the other hand, domestic and low scale milk processing produces whey that has a higher nutritional content and can be utilized locally to produce nutritional drinks that can serve a variety of purposes from refreshment to dietary supplements. Process Guidelines: The research includes collection of waste whey from dairies, messes and canteens; Determination of the proteins and other nutrients present in the whey and their quantity, Extraction and concentration of whey protein and addition of additives; nutritional benefit and waste reduction. The process described above is to be used on a small scale with limited resources both physical as well as monetary hence the current methods of protein extraction from whey fall short. Scope: Whey based nutritional drinks can be produced locally; at low cost; from discarded whey and can play a vital role in combating deficiencies. Whey-Barley beverage contains important nutritional ingredient to address several health problems the common man is facing at the present time. Whey proteins provide excellent nutritional values, in nutrition foods formulated for kids, adults and old aged people as growth tonic for body health maintenance. Additionally, whey protein may help to reduce appetite, improve some diabetes symptoms, increase muscle mass and strength.

The Sailing Stone project animates a stone sculpture such that a traditional, large scale, normally static installation physically interacts with the public. The Sailing Stone moves so as to disrupt the normal motion of visitors to the space so as to encourage changes in the nature of the interaction between visitors and the sculpture itself. The public gallery space is monitored through a network of video cameras, and this information is used to develop a model of human motion through the gallery space. Based on this model of visitor motion, a motion plan is developed and executed for the sculpture so as to maximally disrupt the motion paths of visitors.

We combine traction force data with Bayesian inversion to obtain an absolute estimate of the internal stress field of a cell monolayer. The method, Bayesian inversion stress microscopy (BISM), is validated using numerical simulations performed in a wide range of conditions. It is robust to changes in each ingredient of the underlying statistical model. Importantly, its accuracy does not depend on the rheology of the tissue. We apply BISM to experimental traction force data measured in a narrow ring of cohesive epithelial cells, and check that the inferred stress field coincides with that obtained by direct spatial integration of the traction force data in this quasi-one-dimensional geometry., 38 pages, 14 figures

In speech training aids for providing visual feedback of the articulatory efforts, time-varying vocal tract shape during speech production is generally obtained by linear prediction (LP) analysis of the speech signal and assuming a constant area at the glottis end as a reference. Its variation during speech production causes errors in the estimated vocal tract shape. The problem can be overcome by using area of the mouth opening as the reference. This area can be estimated by detecting the inner lip contour from the video recording of speaker's face during speech utterance. A technique for detection of inner lip contour, based on color transformation and template matching, is presented for reducing the errors caused by presence of teeth and tongue. Face detection by Viola-Jones algorithm, localization using a mouth detection technique, and outer lip contour detection are used to narrow down the search region for inner mouth opening. Presence of the teeth is masked by separate color transformations for upper and lower lip segments. For reducing the errors due to visibility of the tongue, which may not have any significant separation from the lips in the color space, a template matching technique is employed. It is used separately for the upper and lower lip segments to obtain the mouth opening area. The technique has been validated against graphically measured values of the mouth opening and found to be successful in estimating the mouth opening area, and it is not affected by skin hue and presence of teeth.

Simultaneous qualitative and quantitative assessment of eight flavonoids and two terpenoids were performed in fourteen in vitro raised morphogenic cultures of Glycyrrhiza glabra. Our study revealed that the spectrum and production of ten compounds, under investigation, were higher in organized tissue than the undifferentiated mass, however, aerial portions of the in vitro raised plants (leaf and stem) were found to be devoid of therapeutically relevant triterpenoid, glycyrrhizin. A correlation was observed between cell maturation, morphological differentiation and glycyrrhizin accumulation. Mature stolons (4 months) were characterized by the maximum accumulation of glycyrrhizin (8.60 µg/mg) in in vitro plantlets. The cytotoxic effect of the extracts evaluated against a panel of human cancer cell lines (in vitro) indicated that the pancreatic cell line (MIA-PaCa-2) were sensitive to all the fourteen extracts investigated. To the best of our knowledge this is the first comprehensive report relating plant growth regulators to metabolite spectrum and cytotoxic assessment in in vitro raised G. glabra cultures. Overall, our findings demonstrated that the metabolite spectrum of in vitro raised morphogenetic lines, under different stages of maturation, might offer a platform to understand the regulatory aspects of the concerned metabolite pathway and their consequent role in differentiation. Electronic supplementary material The online version of this article (doi:10.1007/s11240-015-0910-4) contains supplementary material, which is available to authorized users.

Rice is one of the major commodities produced and consumed in India. India is also home to one of the finest varieties of rice sold under the name of Basmati. The State of Chhattisgarh is considered the Rice Bowl of India as it is one of the largest contributors to paddy production in India. Around 3 million families subsist on farming in Chhattisgarh, out of which, 1.522 million families are small farmers (owning less than 2 hectares of land). The Government of Chhattisgarh procures paddy in Chhattisgarh on behalf of Government of India. This scheme benefits about 1 million farmer families by procuring about 3 million metric tons of paddy in the Kharif Marketing Season (KMS) of a year, spending about 600 Million US Dollars. The procurement takes place through about 1333 Primary Agricultural Societies in the whole state covering geographical area of 135000 sq. km. The paddy procured is converted into rice by millers after entering into an agreement. Rice is then handed over to Chhattisgarh State Civil Supplies Corporation to use it in another important scheme for providing food security to the poor. The supply chain of rice production is very simple. It is harvested in the form of paddy from the field and sold to the millers who then process the rice. The processed rice is then sold to the market. Although simple, there are a number of inefficiencies present in its supply chain. This case familiarizes the readers of the distribution system of rice, various government policies in its distribution system, and the issues that need to be addressed in a typical distribution system of rice. The case also urges the readers to mull over how these inefficiencies can be overcome with the latest supply chain management techniques so as to make its distribution efficient and cost effective.

A simple model has been planned for human presence sensing in a closed room using a scanning type pyrometric detector, embedded system and intelligent software. Sensors are to be mounted on a rotating platform for scanning of the field of view at regular intervals and at slow speed. This concept is intended to monitor movements of human at desired location and activate equipment accordingly.