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Laser experiments are becoming established as a new tool for astronomical research that complements observations and theoretical modeling. Localized strong magnetic fields have been observed at a shock front of supernova explosions. Experimental confirmation and identification of the physical mechanism for this observation are of great importance in understanding the evolution of the interstellar medium. However, it has been challenging to treat the interaction between hydrodynamic instabilities and an ambient magnetic field in the laboratory. Here, we developed an experimental platform to examine magnetized Richtmyer-Meshkov instability (RMI). The measured growth velocity was consistent with the linear theory, and the magnetic-field amplification was correlated with RMI growth. Our experiment validated the turbulent amplification of magnetic fields associated with the shock-induced interfacial instability in astrophysical conditions for the first time. Experimental elucidation of fundamental processes in magnetized plasmas is generally essential in various situations such as fusion plasmas and planetary sciences., 17 pages, 12 figures, 3 tables, accepted for publication in PRE

Fast heating efficiency when a pre-imploded core is directly heated with an ultraintense laser (heating laser) was investigated. ‘Direct heating’ means that a heating laser hits a pre-imploded core without applying either a laser guiding cone or an external field. The efficiency, η, is defined as the increase in the internal core energy divided by the energy of the heating laser. Six beams (output of 1.6 kJ) from the GEKKO XII (GXII) green laser system at the Institute of Laser Engineering (ILE), Osaka University were applied to implode a spherical deuterated polystyrene (CD) shell target to form a dense core. The DD-reacted protons and the core x-ray emissions showed a core density of 2.8 ± 0.7 g cm−3, or 2.6 times the solid density. Furthermore, DD-reacted thermal neutrons were utilized to estimate the core temperature between 600 and 750 eV. Thereafter, the core was directly heated by a laser for fast-ignition experiments (LFEX, an extremely energetic ultrashort pulse laser) at ILE with its axis lying along or perpendicular to the GXII bundle axis, respectively. The former and latter laser configurations were termed ‘axial’ and ‘transverse modes’, respectively. The η was estimated from three independent methods: (1) the core x-ray emission, (2) the thermal neutron yield, and (3) the runaway hot electron spectra. For the axial mode, 0.8% < η < 2.1% at low power (low LFEX energy) and 0.4% < η < 2.5% at high power (high LFEX energy). For the transverse mode, 2.6% < η < 7% at low power and 1.5% < η < 7.7% at high power. Their efficiencies were compared with that in the uniform implosion mode using 12 GXII beams, 6% < η < 12%, which appeared near to the η for the transverse mode, except that the error bar is very large.

SCD1 is a rate-limiting enzyme in the conversion of saturated fatty acids to monounsaturated fatty acids. SCD1 inhibitors have potential effects on obesity, diabetes, acne, and cancer, but the adverse effects associated with SCD1 inhibition in the skin and eyelids are impediments to clinical development. To avoid mechanism-based adverse effects, we explored the compounds that selectively inhibit SCD1 in the liver in an ex vivo assay. Starting from a systemically active lead compound, we focused on the physicochemical properties tPSA and cLogP to minimize exposure in the off-target tissues. This effort led to the discovery of thiazole-4-acetic acid analog 48 as a potent and liver-selective SCD1 inhibitor. Compound 48 exhibited significant effects in rodent models of diabetes, hepatic steatosis, and obesity, with sufficient safety margins in a rat toxicology study with repeated dosing.

Fast isochoric heating of a pre-compressed plasma core with a high-intensity short-pulse laser is an attractive and alternative approach to create ultra-high-energy-density states like those found in inertial confinement fusion (ICF) ignition sparks. Laser-produced relativistic electron beam (REB) deposits a part of kinetic energy in the core, and then the heated region becomes the hot spark to trigger the ignition. However, due to the inherent large angular spread of the produced REB, only a small portion of the REB collides with the core. Here, we demonstrate a factor-of-two enhancement of laser-to-core energy coupling with the magnetized fast isochoric heating. The method employs a magnetic field of hundreds of Tesla that is applied to the transport region from the REB generation zone to the core which results in guiding the REB along the magnetic field lines to the core. This scheme may provide more efficient energy coupling compared to the conventional ICF scheme., It is desirable to deposit more energy in the dense plasma core to trigger the fusion ignition. Here the authors demonstrate enhanced energy coupling from laser to plasma core by using solid targets and guiding the transport of relativistic electron beam with external magnetic field.

We report the successful fabrication of copper (II) oleate [Cu(C18H33O2)2, Cu-oleate] microspheres as novel targets for laser fusion. Cu-oleate microspheres were prepared using emulsion method with Cu-oleate dissolved in an organic solvent. The fabricated microspheres exhibit a uniform radius of around 103 μm and a good sphericity of 0.97. The microspheres also have high atomic copper (Cu) content of ∼10 wt%, and the Cu ions are homogeneously distributed along their radii. Although defects which are 1-μm deep can be observed on the microsphere surfaces, these defects do not cause any significant perturbation that affects the resulting areal density during implosion. With good morphology, Cu content, and target performance, the Cu-oleate microspheres fabricated by emulsion method can then be used as targets for fast ignition laser fusion experiments such as FIREX. The use of these targets enables the x-ray observation of the laser-induced fast electron energy transfer on the compressed core.

Fast isochoric laser heating is a scheme to heat a matter with relativistic-intensity ($>$ 10$^{18}$ W/cm$^2$) laser pulse or X-ray free electron laser pulse. The fast isochoric laser heating has been studied for creating efficiently ultra-high-energy-density (UHED) state. We demonstrate an fast isochoric heating of an imploded dense plasma using a multi-picosecond kJ-class petawatt laser with an assistance of externally applied kilo-tesla magnetic fields for guiding fast electrons to the dense plasma.The UHED state with 2.2 Peta-Pascal is achieved experimentally with 4.6 kJ of total laser energy that is one order of magnitude lower than the energy used in the conventional implosion scheme. A two-dimensional particle-in-cell simulation reveals that diffusive heating from a laser-plasma interaction zone to the dense plasma plays an essential role to the efficient creation of the UHED state., 8 pages, 4 figures, 1 table

Interfacial instabilities in high-energy-density plasmas are important topics in various research fields such as inertial confinement fusion, planetary sciences, and astrophysics. The growth of the instabilities can be examined in the laboratory by using high-power lasers coupled with high-resolution imaging technique. The resolutions both in space and time are essential for the observation of tiny and transient phenomena in high-energy-density plasmas. Here, the growth of a sinusoidal corrugation on the surface of a polystyrene foil during the laser-driven Richtmyer-Meshkov instability was measured by high-resolution X-ray shadowgraphy. In our experiment, a high-intensity short-pulse laser produced the X-ray flash that ensures a better temporal resolution. A Fresnel Phase Zone Plate was used for the improvement of spatial resolution, which realized an accuracy of 5.0 ± 1.0 µm in our system.

A relativistic laser pulse, confined in a cylindrical-like target, under specific conditions may perform multiple scattering along the internal target surface. This results in the confinement of the laser light, leading to a very efficient interaction. The demonstrated propagation of the laser pulse along the curved surface is just yet another example of the “whispering gallery” effect, although nonideal due to laser–plasma coupling. In the relativistic domain its important feature is a gradual intensity decrease, leading to changes in the interaction conditions. The process may pronounce itself in plenty of physical phenomena, including very efficient electron acceleration and generation of relativistic magnetized plasma structures.

Powerful laser-plasma processes are explored to generate discharge currents of a few $100\,$kA in coil targets, yielding magnetostatic fields (B-fields) in excess of $0.5\,$kT. The quasi-static currents are provided from hot electron ejection from the laser-irradiated surface. According to our model, describing qualitatively the evolution of the discharge current, the major control parameter is the laser irradiance $I_{\mathrm{las}}\lambda_{\mathrm{las}}^2$. The space-time evolution of the B-fields is experimentally characterized by high-frequency bandwidth B-dot probes and by proton-deflectometry measurements. The magnetic pulses, of ns-scale, are long enough to magnetize secondary targets through resistive diffusion. We applied it in experiments of laser-generated relativistic electron transport into solid dielectric targets, yielding an unprecedented 5-fold enhancement of the energy-density flux at $60 \,\mathrm{\mu m}$ depth, compared to unmagnetized transport conditions. These studies pave the ground for magnetized high-energy density physics investigations, related to laser-generated secondary sources of radiation and/or high-energy particles and their transport, to high-gain fusion energy schemes and to laboratory astrophysics., Comment: 11 pages, 7 figures, invited APS

Aim Monoacyglycerol acyltransferases (MGATs) are known to play important roles in intestinal TG absorption. In contrast, the role of MGATs in the liver is still unclear. We investigated the effects of JTP-103237, a novel MGAT inhibitor, on hepatic MGAT activity and hepatic lipid metabolism. Results JTP-103237 reduced hepatic triglyceride content and hepatic MGAT activity in a high sucrose very low fat (HSVLF) diet induced fatty liver model. Interestingly, JTP-103237 suppressed not only triglyceride (TG) and diacylglycerol (DG) synthesis, but also fatty acid (FA) synthesis (de novo lipogenesis) in this model. JTP-103237 also suppressed lipogenesis-related gene expression, such as sterol regulatory element-binding protein 1-c. Moreover, JTP-103237 decreased plasma glucose levels and total cholesterol and reduced the accumulation of epididymal fats in HSVLF diet fed mice. Conclusion In the present study, JTP-103237 prevented carbohydrate-induced fatty liver and suppressed both TG synthesis and de novo lipogenesis, suggesting MGAT inhibitor may prevent carbohydrate-induced metabolic disorders, including NAFLD, obesity and diabetes.

Recent progress in the generation in the laboratory of a strong ($g100$-T) magnetic field enables us to investigate experimentally unexplored magnetohydrodynamics phenomena of a high-energy-density plasma, which an external magnetic field of 200--300 T notably affects due to anisotropic thermal conduction, even when the magnetic field pressure is much lower than the plasma pressure. The external magnetic field reduces electron thermal conduction across the external magnetic field lines because the Larmor radius of the thermal electrons in the external magnetic field is much shorter than the mean free path of the thermal electrons. The velocity of a thin polystyrene foil driven by intense laser beams in the strong external magnetic field is faster than that in the absence of the external magnetic field. Growth of sinusoidal corrugation imposed initially on the laser-driven polystyrene surface is enhanced by the external magnetic field because the plasma pressure distribution becomes nonuniform due to the external magnetic-field structure modulated by the perturbed plasma flow ablated from the corrugated surface.

We investigated the effects of JTT-130 on glucose and lipid metabolism independent of the suppression of feeding by comparing with pair-fed animals. Male Zucker diabetic fatty (ZDF) rats were divided into control, JTT-130 treatment, and pair-fed groups. The rats were fed with a regular powdered diet with or without JTT-130 as a food admixture for 6 weeks. We compared the effects on glucose and lipid metabolism in JTT-130 treatment group with those in pair-fed group.Results. Hyperglycemia in ZDF rats was prevented in both JTT-130 treatment and pair-fed groups, but the prevention in pair-fed group became poor with time. Moreover, reduction in plasma cholesterol levels was observed only in JTT-130 treatment group. JTT-130 treatment group showed improved glucose tolerance at 5 weeks after treatment and significant elevation of portal glucagon-like peptide-1 (GLP-1) levels. The hepatic lipid content in JTT-130 treatment group was decreased as compared with pair-fed group. Furthermore, pancreatic protection effects, such as an increase in pancreatic weight and an elevation of insulin-positive area in islets, were observed after JTT-130 treatment.Conclusions. JTT-130 improves hyperglycemia and dyslipidemia via a mechanism independent of suppression of food intake, which is ascribed to an enhancement of GLP-1 secretion and a reduction of lipotoxicity.

A laboratory measurement of the Zeeman effect in the soft X-ray range can be realized using a laser-produced strong magnetic field and laser-driven magnetic field compression. In this measurement, a pair of laser-driven capacitor-coil targets produces a spatially uniform seed magnetic field of several hundred Tesla, and a low-density SiO2 foam is soaked in the magnetic field. A strongly magnetized high-energy-density SiO2 plasma is then produced by laser-driven compressions of both the foam and the magnetic field. According to a numerical hydrodynamic simulation, a > 10 kT peak magnetic field is achievable with a 100 T seed magnetic field produced by the GEKKO-XII laser facility at Osaka University, Japan. The soft X-ray spectrum is calculated using the MASCB-PPPB code with plasma parameters obtained with the FLASH code. Zeeman splitting of the 4 f − 3 d transition line from lithium-like Si ions of 1.2 eV is observed at 95.4 eV emission peak energy, which is observable even considering the Stark broadening and the spectral resolution of the spectrometer.

Hard X-ray spectroscopy is an essential diagnostics used to understand physical processes that take place in high energy density plasmas produced by intense laser-plasma interactions. A bundle of hard X-ray detectors, of which the responses have different energy thresholds, is used as a conventional single-shot spectrometer for high-flux (>10(13) photons/shot) hard X-rays. However, high energy resolution (Δhv/hv < 0.1) is not achievable with a differential energy threshold (DET) X-ray spectrometer because its energy resolution is limited by energy differences between the response thresholds. Experimental demonstration of a Compton X-ray spectrometer has already been performed for obtaining higher energy resolution than that of DET spectrometers. In this paper, we describe design details of the Compton X-ray spectrometer, especially dependence of energy resolution and absolute response on photon-electron converter design and its background reduction scheme, and also its application to the laser-plasma interaction experiment. The developed spectrometer was used for spectroscopy of bremsstrahlung X-rays generated by intense laser-plasma interactions using a 200 μm thickness SiO2 converter. The X-ray spectrum obtained with the Compton X-ray spectrometer is consistent with that obtained with a DET X-ray spectrometer, furthermore higher certainly of a spectral intensity is obtained with the Compton X-ray spectrometer than that with the DET X-ray spectrometer in the photon energy range above 5 MeV.

Aim: Protein tyrosine phosphatase 1B (PTP1B), a negative regulator of insulin signalling, is a novel therapeutic target for type 2 diabetes mellitus. We evaluated in vitro and in vivo the pharmacological profiles of a new PTP1B inhibitor, JTT-551: monosodium ({[5-(1,1-dimethylethyl)thiazol-2-yl]methyl} {[(4-{4-[4-(1-propylbutyl)phenoxy]methyl}phenyl)thiazol-2-yl]methyl}amino)acetate. Methods: PTP1B inhibitory activity and the inhibition mode were assayed with p-nitrophenyl phosphate as a substrate, and the selectivity of JTT-551 against other PTPs, including T-cell protein tyrosine phosphatase (TCPTP), CD45 protein tyrosine phosphatase (CD45) and leucocyte common antigen-related protein tyrosine phosphatase (LAR), was evaluated. Glucose uptake with JTT-551 treatment was evaluated in L6 rat skeletal myoblasts (L6 cells). In the in vivo study, we investigated the effects on insulin receptor (IR) phosphorylation and blood chemical parameters with JTT-551 administration in ob/ob mice and db/db mice. Results: JTT-551 showed an inhibitory effect on PTP1B with a Ki value of 0.22 µM, and a mixed-type inhibition mode. Ki values of TCPTP, CD45 and LAR were 9.3, 30 or higher and 30 or higher µM, respectively, and JTT-551 exhibited clear selectivity against the other PTPs. Moreover, JTT-551 increased the insulin-stimulated glucose uptake in L6 cells. A single administration of JTT-551 in ob/ob mice enhanced the IR phosphorylation of liver and reduced the glucose level. In db/db mice, chronic administration showed a hypoglycaemic effect without an acceleration of body weight gain. Conclusions: JTT-551, a newly developed PTP1B inhibitor, improves glucose metabolism by enhancement of insulin signalling and could be useful in the treatment of type 2 diabetes mellitus.

A series of experiments were carried out to evaluate the energy-coupling efficiency from heating laser to a fuel core in the fast-ignition scheme of laser-driven inertial confinement fusion. Although the efficiency is determined by a wide variety of complex physics, from intense laser plasma interactions to the properties of high-energy density plasmas and the transport of relativistic electron beams (REB), here we simplify the physics by breaking down the efficiency into three measurable parameters: (i) energy conversion ratio from laser to REB, (ii) probability of collision between the REB and the fusion fuel core, and (iii) fraction of energy deposited in the fuel core from the REB. These three parameters were measured with the newly developed experimental platform designed for mimicking the plasma conditions of a realistic integrated fast-ignition experiment. The experimental results indicate that the high-energy tail of REB must be suppressed to heat the fuel core efficiently.

The study of fast electron spectrum optimization by suppression of preformed plasma in fast ignition targets is presented in this work. Integrated fast-electron spectra for electron energies below 3 MeV—the energy range responsible for core heating—are compared for different preformed plasma conditions. The pulse contrast (the ratio of peak-to-pedestal laser intensities) is compared for 108, 109 and 1011 conditions at constant laser energy (~500 J), pulse duration (2 ps), spot size (30% encircled energy on 50 µm diameter) and laser intensity (around 1 × 1019 W cm−2). The best electron spectrum optimization, consisting of maximized electron number for energies below 3 MeV was obtained with 14 µm thick cone targets. The energy coupling efficiency from heating laser to core plasma, assuming typical core plasma parameters, was estimated to be 2%, although 0.37% was obtained with previous conditions with poor pulse contrast and a 7 µm thick cone target.

A photonuclear-reaction-based hard x-ray spectrometer is developed to measure the number and energy spectrum of fast electrons generated by interactions between plasma and intense laser light. In this spectrometer, x-rays are converted to neutrons through photonuclear reactions, and the neutrons are counted with a bubble detector that is insensitive to x-rays. The spectrometer consists of a bundle of hard x-ray detectors that respond to different photon-energy ranges. Proof-of-principle experiment was performed on a linear accelerator facility. A quasi-monoenergetic electron bunch (Ne = 1.0 × 10(-6) C, Ee = 16 ± 0.32 MeV) was injected into a 5-mm-thick lead plate. Bremsstrahlung x-rays, which emanate from the lead plate, were measured with the spectrometer. The measured spectral shape and intensity agree fairly well with those computed with a Monte Carlo simulation code. The result shows that high-energy x-rays can be measured absolutely with a photon-counting accuracy of 50%-70% in the energy range from 2 MeV to 20 MeV with a spectral resolution (Δhν/hν) of about 15%. Quantum efficiency of this spectrometer was designed to be 10(-7), 10(-4), 10(-5), respectively, for 2-10, 11-15, and 15-25 MeV of photon energy ranges.

The characteristics of oxygen-enriched liquid scintillators with very low afterglow are investigated and optimized for application to a single-hit neutron spectrometer for fast ignition experiments. It is found that 1,2,4-trimethylbenzene has better characteristics as a liquid scintillator solvent than the conventional solvent, p-xylene. In addition, a benzophenon-doped BBQ liquid scintillator is shown to demonstrate very rapid time response, and therefore has potential for further use in neutron diagnostics with fast time resolution.

Protein tyrosine phosphatase 1B (PTP1B) is a negative regulator of leptin signaling as well as insulin signaling. JTT-551 is a new PTP1B inhibitor, which is reported to improve glucose metabolism by enhancement of insulin signaling. We have evaluated an antiobesity effect of JTT-551 using diet-induced obesity (DIO) mice. A single administration of JTT-551 was provided to DIO mice with or without leptin, and DIO mice were given food containing JTT-551 for six weeks. A single administration of JTT-551 with leptin treatment enhanced the food inhibition and the signal transducer and activator of transcription 3 (STAT3) phosphorylation in hypothalamus. Moreover, chronic administration of JTT-551 showed an antiobesity effect and an improvement of glucose and lipid metabolism in DIO mice. JTT-551 shows an antiobesity effect possibly by enhancement of leptin signaling and could be useful in the treatment of type 2 diabetes and obesity.

We found that there are at least five subclasses of N-acetylglucosaminyltransferase I (GnT-I; EC 2.4.1.101) mRNA with different 5′-untranslated regions in rat brain. These five subclasses were also expressed in many tissues with distinct tissue-specific patterns. Moreover, they were regulated differently in response to acute-phase inflammation. The expression of the most abundant subclass of GnT-I mRNA in rat liver decreased 2.5-fold in response to inflammation, concomitantly with a significant decrease in the total amount of GnT-I mRNA. In contrast, one of the minor subclasses of GnT-I mRNA was induced 10-fold by inflammation.

Laser pulse contrast exceeding 1011 was demonstrated on a kilojoule-class petawatt laser for fast ignition experiments (LFEX) laser system [J. Phys. IV France133, 81 (2006)10.1051/jp4:2006133016] by implementing a 2 in. plasma mirror. Laser beams of up to 1.2 kJ striking the plasma mirror with a pulse duration of 1.5 ps were reflected and focused onto a target without significant distortions in the focal spot. Transmitted light from the plasma mirror reveals that it has a high reflectivity 2 ps before the main peak. The estimated laser pulse contrast at the target was 1011 at 1 ns before the main peak. No preformed plasma was observed with an optical interferometry diagnostics, but in the experiment without a plasma mirror a preplasma was clearly observed. The energetic proton was generated from a 0.1 μm thick CH film showing excellent pulse contrast. This technique constitutes a promising method to enhance the LFEX laser system performance in fast ignition experiments.

The dependence of high-energy electron generation on the pulse duration of a high intensity LFEX laser was experimentally investigated. The LFEX laser (λ = 1.054 and intensity = 2.5 – 3 x 1018 W/cm2) pulses were focused on a 1 mm3 gold cubic block after reducing the intensities of the foot pulse and pedestal by using a plasma mirror. The full width at half maximum (FWHM) duration of the intense laser pulse could be set to either 1.2 ps or 4 ps by temporally stacking four beams of the LFEX laser, for which the slope temperature of the high-energy electron distribution was 0.7 MeV and 1.4 MeV, respectively. The slope temperature increment cannot be explained without considering pulse duration effects on fast electron generation.

A petawatt laser for fast ignition experiments (LFEX) laser system [N. Miyanaga et al., J. Phys. IV France 133, 81 (2006)], which is currently capable of delivering 2 kJ in a 1.5 ps pulse using 4 laser beams, has been constructed beside the GEKKO-XII laser facility for demonstrating efficient fast heating of a dense plasma up to the ignition temperature under the auspices of the Fast Ignition Realization EXperiment (FIREX) project [H. Azechi et al., Nucl. Fusion 49, 104024 (2009)]. In the FIREX experiment, a cone is attached to a spherical target containing a fuel to prevent a corona plasma from entering the path of the intense heating LFEX laser beams. The LFEX laser beams are focused at the tip of the cone to generate a relativistic electron beam (REB), which heats a dense fuel core generated by compression of a spherical deuterized plastic target induced by the GEKKO-XII laser beams. Recent studies indicate that the current heating efficiency is only 0.4%, and three requirements to achieve higher efficiency of the fast ignition (FI) scheme with the current GEKKO and LFEX systems have been identified: (i) reduction of the high energy tail of the REB; (ii) formation of a fuel core with high areal density using a limited number (twelve) of GEKKO-XII laser beams as well as a limited energy (4 kJ of 0.53-μm light in a 1.3 ns pulse); (iii) guiding and focusing of the REB to the fuel core. Laser–plasma interactions in a long-scale plasma generate electrons that are too energetic to efficiently heat the fuel core. Three actions were taken to meet the first requirement. First, the intensity contrast of the foot pulses to the main pulses of the LFEX was improved to >109. Second, a 5.5-mm-long cone was introduced to reduce pre-heating of the inner cone wall caused by illumination of the unconverted 1.053-μm light of implosion beam (GEKKO-XII). Third, the outside of the cone wall was coated with a 40-μm plastic layer to protect it from the pressure caused by imploding plasma. Following the above improvements, conversion of 13% of the LFEX laser energy to a low energy portion of the REB, whose slope temperature is 0.7 MeV, which is close to the ponderomotive scaling value, was achieved. To meet the second requirement, the compression of a solid spherical ball with a diameter of 200-μm to form a dense core with an areal density of ∼0.07 g/cm2 was induced by a laser-driven spherically converging shock wave. Converging shock compression is more hydrodynamically stable compared to shell implosion, while a hot spot cannot be generated with a solid ball target. Solid ball compression is preferable also for compressing an external magnetic field to collimate the REB to the fuel core, due to the relatively small magnetic Reynolds number of the shock compressed region. To meet the third requirement, we have generated a strong kilo-tesla magnetic field using a laser-driven capacitor-coil target. The strength and time history of the magnetic field were characterized with proton deflectometry and a B-dot probe. Guidance of the REB using a 0.6-kT field in a planar geometry has been demonstrated at the LULI 2000 laser facility. In a realistic FI scenario, a magnetic mirror is formed between the REB generation point and the fuel core. The effects of the strong magnetic field on not only REB transport but also plasma compression were studied using numerical simulations. According to the transport calculations, the heating efficiency can be improved from 0.4% to 4% by the GEKKO and LFEX laser system by meeting the three requirements described above. This efficiency is scalable to 10% of the heating efficiency by increasing the areal density of the fuel core.

The energy coupling efficiency (CE) in Fast Ignition (FI) laser fusion was studied at GEKKO XII and LFEx laser facility by using newly developed targets and plasma diagnostic instruments. The gated-liquid scintillator neutron detectors had been upgraded by using neutron collimators for intense background fluxes of γ-rays and neutrons in the FI experiment. Clear fusion neutron signal was successfully recorded in the sub-kJ heating FI experiment. Up to 5 times neutron yield enhancement was observed, and the CE of the heating laser to core plasma was estimated to be 1.6% for cone-in-shell target implosion by 9 beams and core heating by LFEX pulse 115 ps before bang time. The laser-to-electron energy conversion efficiency was separately diagnosed using a newly developed target and resulted to be 45%. The fast electron energy spectrum was estimated to be 2.3 MeV slope temperature by hard x-ray spectroscopy. Monte Carlo simulations demonstrate the consistency of the data set.

A kilo-tesla level, quasi-static magnetic field (B-field), which is generated with an intense laser-driven capacitor-coil target, was measured by proton deflectometry with a proper plasma shielding. Proton deflectometry is a direct and reliable method to diagnose strong, mm3-scale laser-produced B-field; however, this was not successful in the previous experiment. A target-normal-sheath-accelerated proton beam is deflected by Lorentz force in the laser-produced magnetic field with the resulting deflection pattern recorded on a radiochromic film stack. A 610 ± 30 T of B-field amplitude was inferred by comparing the experimental proton pattern with Monte-Carlo calculations. The amplitude and temporal evolutions of the laser-generated B-field were also measured by a differential magnetic probe, independently confirming the proton deflectometry measurement results.

It has been known that mouse, rat, and human N-acetylglucosaminyltransferase I (GnT-I) genes produce at least two transcripts, which differ in their 5'-untranslated region (5'-UTR) length, and the longer transcript is preferentially expressed in brains. However, the physiological meaning of this brain-specific expression pattern was unknown. We cloned the rat GnT-I gene and analyzed its structure. It consisted of five exons, and four of them coded only 5'-UTRs. A putative NF-kappaB binding site was found in the 5'-flanking sequence for the transcript that was previously shown to be induced by inflammation. The unusually long 5'-UTR of the major GnT-I transcript in rat brain was shown to inhibit protein production from the following coding sequence in COS7 cells. Comparison of the GnT-I protein/mRNA ratio in rat brain and liver showed that GnT-I mRNA in the brain was translated 3.8-times less efficiently than in the liver. Implications are discussed of these results in regulation of GnT-I expression in rat brain.

A multichannel low-energy neutron spectrometer for down-scattered neutron (DSN) measurements in inertial confinement fusion (ICF) experiments has been developed. Our compact-size 256-channel lithium-glass-scintillator-based spectrometer has been implemented and tested in ICF experiments with the GEKKO XII laser. We have performed time calibration of the 256-channel analog-to-digital convertor system used for DSN measurements via X-ray pulse signals. We have clearly observed the DD-primary fusion neutron signal and have successfully studied the detector's impulse response. Our detector is soon to be implemented in future ICF experiments.

The traditional fast ignition scheme is that a compressed core created using an imploding laser is auxiliary heated and ignited by hot electrons (produced using a short pulse laser guided through the cone). However, sufficient heating cannot be achieved due to the highly hot-electron energy spectra, the energy loss in the cone, the large divergence of the hot electron and the long distance between the core and the generation point. Here, we propose a new target based on a hole-cone shell target experiment.

Diminished insulin sensitivity in the peripheral tissues and failure of pancreatic beta cells to secrete insulin are known major determinants of type 2 diabetes mellitus. JTT-130, an intestine-specific microsomal transfer protein inhibitor, has been shown to suppress high fat-induced obesity and ameliorate impaired glucose tolerance while enhancing glucagon-like peptide-1 (GLP-1) secretion. We investigated the effects of JTT-130 on glucose metabolism and elucidated the mechanism of action, direct effects on insulin sensitivity and glucose-stimulated insulin secretion in a high fat diet-induced obesity rat model. Male Sprague Dawley rats fed a high-fat diet were treated with a single administration of JTT-130. Glucose tolerance, hyperglycemic clamp and hyperinsulinemic-euglycemic testing were performed to assess effects on insulin sensitivity and glucose-stimulated insulin secretion, respectively. Plasma GLP-1 and tissue triglyceride content were also determined under the same conditions. A single administration of JTT-130 suppressed plasma glucose elevations after oral glucose loading and increased the disposition index while elevating GLP-1. JTT-130 also enhanced glucose-stimulated insulin secretion in hyperglycemic clamp tests, whereas increased insulin sensitivity was observed in hyperinsulinemic-euglycemic clamp tests. Single-dose administration of JTT-130 decreased lipid content in the liver and skeletal muscle. JTT-130 demonstrated acute and direct hypoglycemic effects by enhancing insulin secretion and/or insulin sensitivity.

Using the yeast two-hybrid system and an in vitro binding assay, we have identified a novel protein termed vinexin as a vinculin-binding protein. By Northern blotting, we identified two types of vinexin mRNA that were 3 and 2 kb in length. Screening for full-length cDNA clones and sequencing indicated that the two mRNA encode 82- and 37-kD polypeptides termed vinexin alpha and beta, respectively. Both forms of vinexin share a common carboxyl-terminal sequence containing three SH3 domains. The larger vinexin alpha contains an additional amino-terminal sequence. The interaction between vinexin and vinculin was mediated by two SH3 domains of vinexin and the proline-rich region of vinculin. When expressed, vinexin alpha and beta localized to focal adhesions in NIH 3T3 fibroblasts, and to cell-cell junctions in epithelial LLC-PK1 cells. Furthermore, expression of vinexin increased focal adhesion size. Vinexin alpha also promoted upregulation of actin stress fiber formation. In addition, cell lines stably expressing vinexin beta showed enhanced cell spreading on fibronectin. These data identify vinexin as a novel focal adhesion and cell- cell adhesion protein that binds via SH3 domains to the hinge region of vinculin, which can enhance actin cytoskeletal organization and cell spreading.