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Abstract Objective Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) histograms were used to investigate whether their parameters can distinguish between benign and malignant parotid gland tumors and further differentiate tumor subgroups. Materials and methods A total of 117 patients (32 malignant and 85 benign) who had undergone DCE-MRI for pretreatment evaluation were retrospectively included. Histogram parameters including mean, median, entropy, skewness, kurtosis and 10th, 90th percentiles were calculated from time to peak (TTP) (s), wash in rate (WIR) (l/s), wash out rate (WOR) (l/s), and maximum relative enhancement (MRE) (%) mono-exponential models. The Mann–Whitney U test was used to compare the differences between the benign and malignant groups. The diagnostic value of each significant parameter was determined on Receiver operating characteristic (ROC) analysis. Multivariate stepwise logistic regression analysis was used to identify the independent predictors of the different tumor groups. Results For both the benign and malignant groups and the comparisons among the subgroups, the parameters of TTP and MRE showed better performance among the various parameters. WOR can be used as an indicator to distinguish Warthin’s tumors from other tumors. Warthin’s tumors showed significantly lower values on 10th MRE and significantly higher values on skewness TTP and 10th WOR, and the combination of 10th MRE, skewness TTP and 10th WOR showed optimal diagnostic performance (AUC, 0.971) and provided 93.12% sensitivity and 96.70% specificity. After Warthin’s tumors were removed from among the benign tumors, malignant parotid tumors showed significantly lower values on the 10th TTP (AUC, 0.847; sensitivity 90.62%; specificity 69.09%; P

Phenotyping of plant growth improves the understanding of complex genetic traits and eventually expedites the development of modern breeding and intelligent agriculture. In phenotyping, segmentation of 3D point clouds of plant organs such as leaves and stems contributes to automatic growth monitoring and reflects the extent of stress received by the plant. In this work, we first proposed the Voxelized Farthest Point Sampling (VFPS), a novel point cloud downsampling strategy, to prepare our plant dataset for training of deep neural networks. Then, a deep learning network—PSegNet, was specially designed for segmenting point clouds of several species of plants. The effectiveness of PSegNet originates from three new modules including the Double-Neighborhood Feature Extraction Block (DNFEB), the Double-Granularity Feature Fusion Module (DGFFM), and the Attention Module (AM). After training on the plant dataset prepared with VFPS, the network can simultaneously realize the semantic segmentation and the leaf instance segmentation for three plant species. Comparing to several mainstream networks such as PointNet++, ASIS, SGPN, and PlantNet, the PSegNet obtained the best segmentation results quantitatively and qualitatively. In semantic segmentation, PSegNet achieved 95.23%, 93.85%, 94.52%, and 89.90% for the mean Prec, Rec, F1, and IoU, respectively. In instance segmentation, PSegNet achieved 88.13%, 79.28%, 83.35%, and 89.54% for the mPrec, mRec, mCov, and mWCov, respectively.

In the past 3 decades, the cartilage repair potential of mesenchymal stromal cells, or mesenchymal stem cells (MSCs), has been widely examined in animal studies. Unfortunately, the phenotype and physical properties of MSC-derived cartilage tissue are not comparable to native hyaline cartilage. In particular, chondrocytic hypertrophy, a phenotype that is not observed in healthy hyaline cartilage, is concomitant with MSC chondrogenesis. Given that hypertrophic chondrocytes potentially undergo apoptosis or convert into osteoblasts, this undesired phenotype needs to be prevented or minimized before MSCs can be used to repair cartilage injuries in the clinic. In this review, we first provide an overview of chondrocytic hypertrophy and briefly summarize current methods for suppressing hypertrophy in MSC-derived cartilage. We then highlight recent progress on modulating the canonical Wnt/β-catenin pathway for inhibiting hypertrophy. Specially, we discuss the potential crosstalk between Wnt/β-catenin with other pathways in regulating hypertrophy. Lastly, we explore future perspectives to further understand the role of Wnt/β-catenin in chondrocytic hypertrophy.