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Abstract The application of network formulaology and network pharmacology has significantly advanced the scientific understanding of traditional Chinese medicine (TCM) treatment mechanisms in disease. The field of herbal biology is experiencing a surge in data generation. However, researchers are encountering challenges due to the fragmented nature of the data and the reliance on programming tools for data analysis. We have developed TCMNPAS, a comprehensive analysis platform that integrates network formularology and network pharmacology. This platform is designed to investigate in-depth the compatibility characteristics of TCM formulas and their potential molecular mechanisms. TCMNPAS incorporates multiple resources and offers a range of functions designed for automated analysis implementation, including prescription mining, molecular docking, network pharmacology analysis, and visualization. These functions enable researchers to analyze and obtain core herbs and core formulas from herbal prescription data through prescription mining. Additionally, TCMNPAS facilitates virtual screening of active compounds in TCM and its formulas through batch molecular docking, allowing for the rapid construction and analysis of networks associated with “herb-compound-target-pathway” and disease targets. Built upon the integrated analysis concept of network formulaology and network pharmacology, TCMNPAS enables quick point-and-click completion of network-based association analysis, spanning from core formula mining from clinical data to the exploration of therapeutic targets for disease treatment. TCMNPAS serves as a powerful platform for uncovering the combinatorial rules and mechanism of TCM formulas holistically. We distribute TCMNPAS within an open-source R package at GitHub ( https://github.com/yangpluszhu/tcmnpas ), and the project is freely available at http://54.223.75.62:3838/ .

BackgroundPrecise preoperative evaluation of lymph node metastasis (LNM) is crucial for ensuring effective treatment for rectal cancer (RC). This research aims to develop a clinical-radiomics nomogram based on deep learning techniques, preoperative magnetic resonance imaging (MRI) and clinical characteristics, enabling the accurate prediction of LNM in RC.Materials and methodsBetween January 2017 and May 2023, a total of 519 rectal cancer cases confirmed by pathological examination were retrospectively recruited from two tertiary hospitals. A total of 253 consecutive individuals were selected from Center I to create an automated MRI segmentation technique utilizing deep learning algorithms. The performance of the model was evaluated using the dice similarity coefficient (DSC), the 95th percentile Hausdorff distance (HD95), and the average surface distance (ASD). Subsequently, two external validation cohorts were established: one comprising 178 patients from center I (EVC1) and another consisting of 88 patients from center II (EVC2). The automatic segmentation provided radiomics features, which were then used to create a Radscore. A predictive nomogram integrating the Radscore and clinical parameters was constructed using multivariate logistic regression. Receiver operating characteristic (ROC) curve analysis and decision curve analysis (DCA) were employed to evaluate the discrimination capabilities of the Radscore, nomogram, and subjective evaluation model, respectively.ResultsThe mean DSC, HD95 and ASD were 0.857 ± 0.041, 2.186 ± 0.956, and 0.562 ± 0.194 mm, respectively. The nomogram, which incorporates MR T-stage, CEA, CA19-9, and Radscore, exhibited a higher area under the ROC curve (AUC) compared to the Radscore and subjective evaluation in the training set (0.921 vs. 0.903 vs. 0.662). Similarly, in both external validation sets, the nomogram demonstrated a higher AUC than the Radscore and subjective evaluation (0.908 vs. 0.735 vs. 0.640, and 0.884 vs. 0.802 vs. 0.734).ConclusionThe application of the deep learning method enables efficient automatic segmentation. The clinical-radiomics nomogram, utilizing preoperative MRI and automatic segmentation, proves to be an accurate method for assessing LNM in RC. This approach has the potential to enhance clinical decision-making and improve patient care.Research registration unique identifying number (UIN)Research registry, identifier 9158, https://www.researchregistry.com/browse-the-registry#home/registrationdetails/648e813efffa4e0028022796/.

Abstract Long timber columns are widely used in traditional timber structures worldwide; however, many of them possess bearing capacity issues and have to be strengthened. The current study aims to develop the novel Hybrid fiber reinforced polymers (HFRP) sheets suitable for long timber columns. In the first stage, six HFRP sheets were designed, and the evaluation of these sheets was carried out by the tensile tests and the Scanning electron microscopy (SEM) tests. Secondly, for the compression test, two sets of 36 timber columns were designed utilizing six types of HFRP sheets. Finally, the effects of different sheet types and strengthening methods on the compressive performance of timber columns were investigated. The findings reveal that bidirectional HFRP sheets have excellent compressive performance for timber columns. In the case of using one-layer interval reinforcement, the bearing capacity can be increased by 22.27%, which is 12.15–17.81% higher than that of the unidirectional HFRP sheets. Compared with one-layer reinforcement, the two-layer reinforcement increased from 0.91% to 5.35%. The current study's findings are intended to provide an essential scientific foundation for the advancement of fiber-reinforced timber column technology and the preservation of the heritage of timber structures.

Abstract Background Quick contrast sensitivity function (qCSF) method is an advanced quick method for contrast sensitivity function (CSF) evaluation. This study evaluated the contrast sensitivity (CS) of eyes undergoing cataract surgery with multifocal intraocular lens (IOL) implantation and its tolerance to IOL tilt and IOL decentration using the qCSF method. Methods Patients undergoing uneventful phacoemulsification and a trifocal IOL (Zeiss AT LISA tri 839MP, Carl Zeiss, Germany) or an extended depth-of-focus (EDOF) IOL (Tecnis Symfony ZXR00, Johnson & Johnsons, USA) implantation were included. Monocular contrast sensitivity was measured using the qCSF method at one month post-surgery. IOL tilt and decentration were measured using an optical aberrometer (OPD-Scan III, NIDEK, Japan). Results Seventy-two patients/eyes with the 839MP IOL and 64 patients/eyes with the ZXR00 IOL were included. Area under the log CSF (AULCSF) and CS acuity did not differ significantly between the two groups. The ZXR00 IOL group showed better CS at 1 cpd (1.137 ± 0.164 vs. 1.030 ± 0.183 logCS) and 1.5 cpd (1.163 ± 0.163 vs. 1.071 ± 0.161 logCS), while the 839MP IOL group had better CS at 6 cpd (0.855 ± 0.187 vs. 0.735 ± 0.363 logCS). In the 839MP IOL group, all CSF metrics were negatively correlated with IOL tilt (all P 0.05). Among myopic eyes, fewer CSF metrics were negatively correlated with IOL tilt in the ZXR00 IOL group than in the 839MP IOL group. No significant correlation was found between CSF metrics and IOL decentration. Conclusions The ZXR00 and the 839MP IOL groups presented comparable CSF. CS was negatively correlated with IOL tilt, instead of decentration in multifocal IOLs, particularly among myopic eyes. The ZXR00 IOL had better tolerance to IOL tilt in myopic eyes.

By examining structures, sediments, reservoirs and accumulation assemblages in the Deyang—Anyue rift and its surrounding area, four new understandings are obtained. First, during the initiation period of Deyang—Anyue rift, multiple groups of faults developed in the rift due to the effect of tensile force, bringing about multiple mound and shoal belts controlled by horsts in the second member of the Sinian Dengying Formation; in the development stage of the rift, the boundary faults of the rift controlled the development of mound and shoal belts at the platform margin in the fourth member of Dengying Formation; during the shrinkage period of the rift, platform margin grain shoals of the Cambrian Canglangpu Formation developed in the rift margin. Second, four sets of large-scale mound and shoal reservoirs in the second member of Dengying Formation, the fourth member of Dengying Formation, Canglangpu Formation and Longwangmiao Formation overlap with several sets of source rocks such as Qiongzhusi Formation source rocks and Dengying Formation argillaceous limestone or dolomite developed inside and outside the rift, forming good source-reservoir-cap rock combinations; the sealing of tight rock layers in the lateral and updip direction results in the formation model of large lithologic gas reservoirs of oil pool before gas, continuous charging and independent preservation of each gas reservoir. Third, six favorable exploration zones of large-scale lithologic gas reservoirs have been sorted out through comprehensive evaluation, namely, mound and shoal complex controlled by horsts in the northern part of the rift in the second member of Dengying Formation, isolated karst mound and shoal complex of the fourth member of Dengying Formation in the south of the rift, the superimposed area of multi-stage platform margin mounds and shoals of the second and fourth members of Dengying Formation and Canglangpu Formation in the north slope area, the platform margin mounds and shoals of the second and fourth members of Dengying Formation in the west side of the rift, the platform margin mound and shoal bodies of the fourth member of Dengying Formation in the south slope area, etc. Fourth, Well Pengtan-1 drilled on the mound and shoal complex controlled by horsts of the second member of Dengying Formation in the rift and Well Jiaotan-1 drilled on the platform margin mound and shoal complex of the North Slope have obtained high-yield gas flows in multiple target layers, marking the discovery of a new gas province with reserves of (2—3)×1012 m3. This has proved the huge exploration potential of large lithologic gas reservoir group related to intracratonic rift.

Abstract Background The Alphapapillomavirus 9 (α-9 HPV) is a member of the Alphapapillomavirus genus and Papillomaviridae family. These viruses are almost all carcinogenic HPV, which is closely related to 75% of invasive cervical cancer worldwide, and has a high prevalence in Sichuan. The carcinogenic function is mainly realized by its E6 oncoprotein. Methods Cell samples were collected by cervical scraped for HPV detecting and typing. HPV-16, HPV-31, HPV-33, HPV-52, HPV-58 5 α-9 genus HPV subtype positive samples were selected, their E6 gene was sequenced and analyzed. The positive selection sites of HPV E6 genes were estimated by PAML 4.8 server. The secondary and tertiary structure of E6 protein were predicted by PSIPred and Swiss-model. The T-cell antigen epitopes of E6 protein were predicted by IEDB. Results α-9 HPV has a high prevalence in Sichuan, China. From 2012 to 2017, 18,067 cell cervical samples were collected, and 3135 were detected with α-9 HPV infection. Among which, 250 cases HPV-16 E6, 96 cases HPV-31 E6, 216 cases HPV-33 E6, 288 cases HPV-52 E6 and 405 cases HPV-58 E6 were successfully amplified, 17, 6, 6, 13, and 4 non-synonymous nucleotide mutations were respectively detected in HPV-16, 31, 33, 52, and 58 E6, 7 positive selection sites of α-9 HPV E6 were selected out (D32E of HPV-16 E6, K35N, K93N and R145I of HPV-33 E6, K93R of HPV-52 E6, K93N and R145K of HPV-58 E6). The structure and antigen epitopes of E6 protein with amino acid substitution differ from those of wild-type E6 protein, especially for the mutation located in the E6 positive selection site. Conclusions HPV E6 nucleotide non-synonymous mutation in the positive selection site influence the protein structure and decrease the antigen epitopes affinity of the E6 protein overall, making it more difficult for the HPV-infected cells to be detected by the immune system, and enhancing the HPV adaptability to the environment. Mutations influence the validity of HPV clinical diagnostic probes, the polymorphism analysis of α-9 HPV E6 enrich the data of HR-risk HPV in Sichuan China, and the detection probes designed with the polymorphism data in mind can improve the efficiency of clinical detection; Mutations influence epitopes affinity, the association of E6 polymorphism and epitope affinity can improve the design of therapeutic vaccine with good immunity and high generality antigen epitope; The above study all provide a good theoretical basis for the prevention and treatment of HPV-related diseases.

With the COVID-19 pandemic sweeping the world, the development of China's energy industry has been hampered. Although previous studies have shown the global influence of COVID-19 on energy prices and macroeconomic indicators, very few of them examined the impact on China independently, considering the special role of China in this pandemic and economy. In this study, we investigate the impact of the pandemic on several major China energy prices using the ARIMA-GARCH model. Combined with the Value-at-Risk (VaR) theory, we further explore the market risk, which indicates an increase in the tail risk of energy price volatility and the dramatic turbulence in energy markets. In addition, a Vector Autoregressive (VAR) model is developed to analyze how the main macroeconomic indicators are affected when energy prices fluctuate. According to the model results, energy price fluctuations caused by the COVID-19 have a negative impact on economic growth and inflation, with a higher contribution to the latter changes. Based on the modeling analysis results, this paper makes constructive suggestions on how to stabilize energy prices and recover the economic development in the context of the COVID-19 pandemic.

Metal-organic frameworks (MOFs) with porosity and functional adjustability have great potential for the removal of organic dyes in the wastewater. Herein, an anionic porous metal-organic framework (MOFs) [Me2NH2]2In2[(TATAB)4(DMF)4]·(DMF)4(H2O)4 (HDU-1) was synthesized, which is constructed from a [In(OOC)4]− cluster and a nitrogen-rich linker H3TATAB (4,4′,4″-s-triazine-1,3,5-triyltri-p-aminobenzoic acid). The negatively charged [In(OOC)4]− cluster and uncoordinated –COOH on the linker result in one unit cell of HDU-1 having 8 negative sites. The zeta potential of -20.8 mV dispersed in pure water also shows that HDU-1 possesses negatively charged surface potential. The high electronegativity, water stability, and porosity of HDU-1 can facilitate the ion-exchange and Coulombic interaction. As expected, the HDU-1 exhibits high selectivity and removal rates towards trace cationic dyes with suitable size, such as methylene blue (MB) (96%), Brilliant green (BG) (99.3%), and Victoria blue B (VB) (93.6%).

Underwater shield tunneling will disturb the soil near the river, especially in water-rich soft ground. This may cause a groundwater infiltration hydraulic gradient to exceed the critical value, leading to calamities, such as unexpected flooding or submerged erosion. To ensure the security of construction and the stability of river embankment seepage, it is crucial to assess the safety of the underwater tunnel cover thickness. A shield tunnel project under a river in Hefei is used as an example. The numerical model established by the finite element method is used for calculating and analyzing the changes in the groundwater flow field and the stability state of embankment seepage induced by underwater shield tunneling under different overburden thickness conditions. The results show that the construction disturbance of the shield tunnel through the river is increased, the internal force environment of the embankment slope is destroyed, and the maximum seepage hydraulic gradient is increased. In the case study, the embankment keeps in a stable state of seepage when the cover thickness of the shield tunnel has 2.9 times its outer diameter. The findings of this study can serve as a scientific guide to assure seepage stability in an underwater shield tunneling project and to stop river embankment erosion.

Drilling, seismic and logging data were used to evaluate the hydrocarbon accumulation conditions of the mound-shoal complexes in the platform margin of the fourth member of Sinian Dengying Formation in the east side of the Mianzhu-Changning intracratonic rift in the Sichuan Basin. The four understandings are: (1) The platform margin belt of the Deng 4 Member can be divided into three sections, northern, middle and southern; the middle section is at the core of the Gaoshiti-Moxi paleouplift and the structural high now, while the southern and northern sections are at the slope of the paleouplift and the structural lows now; the three sections have similar development characteristics and reservoir features of platform margin mound-shoal complex. (2) In the margin of the east side of the rift, there are several faults nearly perpendicular to the platform margin belt, the faults divide the platform margin belt into rugged paleo-landform, and the high part developed platform margin mound-shoal complexes and the reservoirs are good in physical properties, while the low part developed inter-beach depression and no mound-shoal complexes, where the reservoirs are poor in physical properties. (3) The six groups of faults nearly perpendicular to the platform margin belt divide the platform margin belt into seven large mound-shoal complexes which have similar hydrocarbon accumulation conditions and accumulation evolution process and are rich in petroleum. (4) The inter shoal depressions between the mound-shoal complexes are characterized by tighter lithology, which can block the updip direction of the mounds and shoals at the lower part of the slope of the paleouplift and are favorable for the later preservation of mound-shoal gas reservoirs. This has been proved by Well Jiaotan 1 and Heshen 2 drilled successfully. The mound-shoal complexes on the platform margin of the structural slope area have a good exploration prospect.

Abstract Li‐CO2 battery has attracted extensive attention and research due to its super high theoretical energy density and its ability to fix greenhouse gas CO2. However, the slow reaction kinetics during discharge/charge seriously limits its development. Hence, a simple cation exchange strategy is developed to introduce Ru atoms onto a Co3O4 nanosheet array grown on carbon cloth (SA Ru‐Co3O4/CC) to prepare a single atom site catalyst (SASC) and successfully used in Li‐CO2 battery. Li‐CO2 batteries based on SA Ru‐Co3O4/CC cathode exhibit enhanced electrochemical performances including low overpotential, ultra high capacity, and long cycle life. Density functional theory calculations reveal that single atom Ru as the driving force center can significantly enhance the intrinsic affinity for key intermediates, thus enhancing the reaction kinetics of CO2 reduction reaction in Li‐CO2 batteries, and ultimately optimizing the growth pathway of discharge products. In addition, the Bader charge analysis indicates that Ru atoms as electron‐deficient centers can enhance the catalytic activity of SA Ru‐Co3O4/CC cathode for the CO2 evolution reaction. It is believed that this work has important implications for the development of new SASCs and the design of efficient catalyst for Li‐CO2 batteries.

Natural gas in the gas reservoirs of Lower Cambrian Longwangmiao Fm is mainly concentrated in the granular shoal facies in central Sichuan Basin, therefore the study on lithofacies paleogeography and the prediction on granular shoal distribution are the key to improving the success ratio of natural gas exploration in this area. In this paper, outcrop, drilling, seismic and logging data were studied comprehensively. Subsequently, the sedimentary facies and sedimentary model of Longwangmiao Formation in the Sichuan Basin and its adjacent areas were studied, and lithofacies paleogeographic maps were prepared based on previous research results. Besides, “three-shoal” sedimentary model of Longwangmiao Fm was established, the distribution range of three shoal belts was predicted and their development characteristics and main controls were analyzed, thereby pointing out the direction and field of natural gas exploration in the next stage. It is indicated from the research results that: first, the paleogeographic pattern of the Sichuan Basin in the Longwangmiao period was represented as a rimed carbonate rock platform, with the developments of sedimentary facies such as mixed tidal flat, restricted platform, open platform, platform margin and slope facies. Second, the “three-shoal” sedimentary model of Longwangmiao Fm was established. One of them is the granular shoal in platform margin facies and is distributed along the eastern margin of the Sichuan Basin. The other two are intra-platform granular shoals which were distributed on the east and west sides of lagoon subfacies, belonging to restricted platform facies and open platform facies, respectively. Third, the granular shoal of restricted platform facies is the main reservoir of Longwangmiao Fm gas reservoir in the Anyue Gas Field. It is distributed in central Sichuan, and its development is controlled by lagoons and Gaoshiti–Moxi Paleo-uplift. Fourth, the platform margin granular shoal is mainly distributed along the northern and eastern margins of the basin, and its development is controlled by the slope break of sedimentary paleogeomorphy. In conclusion, the granular shoal of restricted platform facies and shoal of platform margin facies are good reservoirs that they are the important fields of natural gas exploration. Furthermore, the new understanding to “three-shoal” sedimentary model and granular shoal distribution of Longwangmiao Fm is of great theoretical significance to the study on sedimentary systems of ancient strata. Keywords: Sichuan Basin, Early Cambrian, Longwangmiao Fm, Sedimentary facies, Lithofacies paleogeography, Restricted platform, Platform margin, Granular shoal, Natural gas exploration

Objective: Oxidative stress and apoptosis play critical roles in the pathogenesis of heart failure (HF).Nuanxin capsule (NX) is a Chinese medicine that has outstanding protective effects on HF. The present study aimed to elucidate whether NX could protect HF against oxidative stress-induced apoptosis through intrinsic mitochondrial pathway. Methods: In vivo, HF was induced by transverse aortic constriction. NX and Compound C (Comp C) were administered to C57BL/6 J mice for over a 4-week period. Cardiac function was assessed with echocardiography. In vitro, H9c2 cells were exposed to H2O2 in the presence or absence of NX and Compound C. Cell viability, cytotoxicity, reactive oxygen species (ROS) production, apoptosis, mitochondrial membrane potential (ΔΨm) and mitochondrial function by oxygen consumption rate (OCR) were detected. The expressions of cytochrome c, BAX, Bcl-2, cleaved caspase-3, AMPK and JNK were evaluated by western blotting. Results: The results indicated that NX significantly improved cardiac function and enhanced the cell viability, ΔΨm and mitochondrial respiration. Also NX treatment reduced cell cytotoxicity and ROS production. Moreover, NX inhibited mitochondrial-mediated apoptosis by upregulating AMPK and downregulating JNK both in vivo and in vitro. The protective effects of NX on cardiac function by reducing oxidative stress-induced mitochondrial dependent apoptosis were reversed by Compound C treatment. Conclusions: These findings demonstrated that NX effectively improved cardiac function in TAC mice by reducing oxidative stress-induced mitochondrial dependent apoptosis by activating AMPK/JNK signaling pathway.

Left atrial (LA) fibrosis is a major arrhythmogenic substrate for atrial fibrillation (AF). The purpose of this study was to assess whether isoproterenol (ISO) induces LA fibrosis and increases susceptibility to AF, exploring the underlying mechanisms. Male Sprague-Dawley rats were subcutaneously injected ISO once per day for 2 days. Five weeks after injection, the ISO group had higher susceptibility AF and prolonged AF duration compared with the control group. ISO decreased LA conduction velocity (CV) and increased LA conduction heterogeneity. ISO increased fibrosise areas and the protein levels of collagen types I and III in the left atrium. Antifibrosis drug pirfenidone decreased AF occurrence and reduced LA fibrosis in ISO treated rats. ISO injection induced atrial ischemia infarction by increasing heart rate and decreasing diastolic and systolic blood pressures. These findings demonstrated that ISO increases susceptibility to AF by increasing LA fibrosis and LA conduction abnormalities 5 weeks after injection. ISO injection induces atrial ischemic injury is the main cause of fibrosis. Rats with ISO-induced LA fibrosis may be used in further AF research.

Objective: Tongguan Capsule, a traditional Chinese medicine, is safe to use and is efficient in treating ischemic heart diseases. The present study aimed to investigate whether Tongguan capsule derived-herb (TGD) can mitigate left ventricular remodeling and dysfunction in post myocardial infarction (MI) rats as well as reduce arrhythmias. Design and methods: MI was induced by a ligation of the left anterior descending coronary artery. TGD was administered to the post-MI rats over a period of 4 weeks. TGD treatment significantly attenuated tachyarrhythmia inducibility and cardiac dysfunction in post-MI heart. Echocardiogram showed that TGD significantly reduced the development of ventricular remodeling. Histological study revealed that TGD significantly reduced myocardial interstitial collagen deposition, myocyte area and α-smooth muscle actin (α-SMA) expression, and increased connexin 43 expression in the infarcted border zone (IBZ). Western blotting results revealed that TGD treatment significantly down-regulated the protein expression levels of type I and III collagen, α-SMA, and up-regulated connexin 43. RT-qPCR results showed that TGD decreased the levels of ANP and BNP. Conclusions: These findings provided strong evidences that TGD intervention ameliorated interstitial fibrosis, myocyte hypertrophy and gap junction expression in the IBZ, attenuated left ventricular remodeling and dysfunction, and reduced vulnerability to tachyarrhythmia. TGD inhibited IBZ remodeling by its inhibition effect on myofibroblasts differentiation.

White LEDs were encapsulated using Cs4PbBr6 quantum dots and Gd2O3:Eu red phosphor as lamp powder. Under the excitation of a GaN chip, the color coordinates of the W-LED were (0.33, 0.34), and the color temperature was 5752 K, which is close to the color coordinate and color temperature range of standard sunlight. The electric current stability was excellent with an increase in the electric current, voltage, and luminescence intensity of the quantum dots and phosphors by more than 10 times. However, the stability of the quantum dots was slightly insufficient over long working periods. The photocatalytic devices were constructed using TiO2, CsPbBr3, and NiO as an electron transport layer, light absorption layer, and catalyst, respectively. The Cs–Pb–Br-based perovskite quantum dot photocatalytic devices were constructed using a two-step spin coating method, one-step spin coating method, and full PLD technology. In order to improve the water stability of the device, a hydrophobic carbon paste and carbon film were selected as the hole transport layer. The TiO2 layer and perovskite layer with different thicknesses and film forming qualities were obtained by changing the spin coating speed. The influence of the spin coating speed on the device’s performance was explored through SEM and a J–V curve to find the best spin coating process. The device constructed by the two-step spin coating method had a higher current density but no obvious increase in the current density under light, while the other two methods could obtain a more obvious light response, but the current density was very low.

Seismic, drilling, logging and production performance data were analyzed to study the key geological factors such as reservoir properties and gas/water distribution, which influence the development of the Lower Cambrian Longwangmiao reservoirs in the Moxi block, Anyue gas field, Sichuan Basin, and the development strategies are established. The results indicate that: (1) Four stages of grain shoals developed longitudinally and two main shoals with one trench formed laterally in the development area; (2) Three types of reservoirs are identified, which are reservoir with millimeter sized dissolved vugs, reservoir with solution pores, and reservoir with inter-particle/inter-crystal pores; (3) Low matrix porosity but mid-to-high permeability affected by developed high-dip fractures; and (4) Three gas/water contacts (GWC) can be defined and step down from west to east, with a unique gas/water contact of −4 385 m in the target developing area. Based on geologic characterization, reservoir simulation and case study of similar gas reservoirs, the development strategies are made: to select the main grain shoal area as the prior production area; to locate wells on the structural high to delay edge water breakthrough and prolong stable production; to drill horizontal wells enhancing single well deliverability; to keep reasonable production of wells for improving the production effect. Key words: Anyue gas field, Moxi block, Longwangmiao Formation, carbonate gas reservoir, geological characteristics, development strategies

Abstract Invasive alien plant effects on ecosystem functions are often difficult to predict across environmental gradients due to the context‐dependent interactions between the invader and the recipient communities. Adopting a functional trait‐based framework could provide more mechanistic predictions for invasive species' impacts. In this study, we contrast litter decomposition rates among communities with and without the invasive plant Impatiens glandulifera in five regions along a 1600 km long latitudinal gradient in Europe. Across this gradient, four functional traits, namely leaf dry matter content (LDMC), specific leaf area (SLA), stem‐specific density (SSD), and plant height, are correlated to rates of litter decomposition of standardized rooibos (labile), green tea (recalcitrant), and I. glandulifera litter. Our results show that both invaded and non‐invaded plant communities had a higher expression of acquisitive traits (low LDMC and SSD, high SLA) with increasing temperature along the latitudinal gradient, partly explaining the variation in decomposition rates along the gradient. At the same time, invasion shifted community trait composition toward more acquisitive traits across the latitudinal gradient. These trait changes partly explained the increased litter decomposition rates of the labile litter fraction of rooibos and I. glandulifera litter in invaded communities, a shift that was most evident in the warmer study regions. Plant available nitrogen was lower in invaded communities, likely due to high nutrient uptake by I. glandulifera. Meanwhile, the coldest study region was characterized by a reversed effect of invasion on decomposition rates. Here, community traits related to low litter quality and potential allelopathic effects of the invader resulted in reduced litter decomposition rates, suggesting a threshold temperature at which invader effects on litter decomposition turn positive. This study therefore illustrates how functional trait changes toward acquisitive traits can help explain invader‐induced changes in ecosystem functions such as increased litter decomposition.

Water splitting to produce hydrogen is known as an effective way to alleviate the energy crisis, but the slow kinetics of the oxygen evolution reaction (OER) has been seriously restricting the development of water splitting technology. Therefore, low cost and high efficiency OER electrocatalysts have become substitutes for traditional noble metal-based catalysts. In this work, CuCoO2 nanosheets (denoted by CCO2) were successfully synthesized under the regulation of surfactants and a solvent polyethylene glycol (PEG) by a solvothermal route using Cu-BTC and Co(NO3)2·6H2O as reactants. The experimental results confirmed that PEG addition could further reduce significantly the crystal size of the CCO2 nanosheets, i.e., the size was about 150 nm and the thickness was 13 nm. The Ni@CCO2 electrode exhibits outstanding OER performance in 1.0 M KOH electrolyte, which shows the overpotential at 10 mA cm-2 is 378 mV, and the Tafel slope is 85 mV dec-1. Moreover, the CCO2 nanosheets exhibit good structural and compositional stability after the 18 h constant current OER test. Therefore, this work may offer a novel insight into enhancing the OER performance of CuCoO2 catalysts by decreasing their crystal size, and using a solvothermal route. [ABSTRACT FROM AUTHOR]

Abstract Bladder cancer (BCa) stands out as a highly prevalent malignant tumor affecting the urinary system. The Sex determining region Y-box protein family is recognized for its crucial role in BCa progression. However, the effect of Sex determining region Y-box 7 (SOX7) on BCa progression has not been fully elucidated. Herein, RNA-sequencing, western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF) and tissue microarray were utilized to assess SOX7 expression in vitro and in vivo. Additionally, SOX7 expression, prognosis, and SOX7 + cytoglobin (CYGB) score were analyzed using R software. In vitro and vivo experiments were performed with BCa cell lines to validate the effect of SOX7 knockdown and overexpression on the malignant progression of BCa. The results showed that SOX7 exhibits low expression in BCa. It functions in diverse capacities, inhibiting the proliferative, migratory, and invasive capabilities of BCa. In addition, the experimental database demonstrated that SOX7 binds to the promoter of DNA methyltransferase 3 beta (DNMT3B), leading to the transcriptional inhibition of DNMT3B. This subsequently results in a reduced methylation of CYGB promoter, ultimately inhibiting the tumor progression of BCa. SOX7 + CYGB scores were significantly linked to patient prognosis. In conclusion, SOX7 inhibits the malignant progression of BCa via the DNMT3B/CYGB axis. Additionally, the SOX7 + CYGB score is capable of predicting the prognostic outcomes of BCa patients. Therefore, SOX7 and CYGB may play an important role in the progression of bladder cancer, and they can be used as prognostic markers of bladder cancer patients.