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27. Evaluation of the restorative effect of ozone and chitosan–hyaluronic acid with and without mesenchymal stem cells on wound healing in rats.

Author

Shabani, Mahshid, Sharifi, Siavash, Karimi, Iraj, Shirian, Sadegh, Fadaei, Milad, and Mirzaei, Esmaeil

Subjects
MESENCHYMAL stem cells, WOUND healing, OZONE, EPITHELIUM, RATS
Abstract

This study evaluated the effect of ozone, chitosan–hyaluronic (Cs–HA) acid and mesenchymal stem cells (MSCs) on wound healing in rats. A total of 64 rats were randomly divided into four groups: control, ozone, Cs–HA + ozone and Cs–HA + ozone + MSCs. A 5 mm full‐thickness wound was created on the back of each rat. The wound area was measured macroscopically on days 3, 5, 9 and 14. Tissue sections were prepared for histopathological evaluation of inflammation, collagen arrangement, neovascularization and epithelial tissue rearrangement. Macroscopic assessment showed differences in wound area on days 5, 9 and 14. Histopathological examination showed that the Cs–HA + ozone + MSCs and Cs–HA + ozone groups had significantly higher vascularization on day 3 compared to the ozone‐treated and control groups. All treatment groups had significantly better collagen arrangement than the control group. On day 5, no significant difference was observed between different groups. On day 9, the inflammation level in the Cs–HA + ozone + MSCs group was significantly lower than in the other groups. All treatment groups had significantly better vascularization compared to the control group. On day 14, the rate of inflammation was significantly lower in the treatment groups than in the control group. Significantly higher collagen arrangement levels were observed in the Cs–HA + ozone and Cs–HA + ozone + MSCs groups compared to the control and ozone groups. All treatment groups had significantly better epithelial tissue rearrangement than the control group. Overall, the results of this study indicated that treatment with ozone, Cs–HA acid, Cs–HA and MSCs accelerated wound healing in rats. The effect of using Cs–HA acid with mesenchymal cells was better than the other types of treatment. Larger clinical trials are needed to assess these factors for improving chronic wound treatment. [ABSTRACT FROM AUTHOR]

Published
2024
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28. The evaluation of complete blood count parameters in the patients with idiopathic versus secondary cerebral venous thrombosis.

Author

Gholamzadeh, Mohammad Javad, Hooshmandi, Etrat, Ghahramani, Zahra, Fereidooni, Reza, Rezvani, Alireza, Vasaghi-Gharamaleki, Maryam, Molavi-Vardanjani, Hossein, Shirian, Sadegh, Fadakar, Nima, Ostovan, Vahid Reza, Poursadeghfard, Maryam, Ashjazadeh, Nahid, and Borhani-Haghighi, Afshin

Subjects
BLOOD cell count, CEREBRAL embolism & thrombosis, VENOUS thrombosis, ERYTHROCYTES, BIOMARKERS
Abstract

Background: Several laboratory markers derived from a complete blood count (CBC) have been proposed as potential indicators for assessing the risk of cerebral venous thrombosis (CVT). However, limited and conflicting evidence exists regarding this association. This study aimed to evaluate the role of CBC parameters in CVT development and their link to disease characteristics. Methods: This case-control study included patients diagnosed with CVT between March 2018 and March 2021. [ABSTRACT FROM AUTHOR]

Published
2024
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29. Tissue Engineering Using Differentiation of Endometrial Stem Cells into Ameloblast Cells and Implantation on Hydroxyapatite and Collagen Nanocomposite Scaffold.

Author

Bahrami, Naghmeh, Babaei, Azadeh, Shirian, Sadegh, Ai, Arman, Mohamadnia, Abdolreza, Shafaghi, Shadi, Pishbin, Asma, and Ai, Jafar

Subjects
STEM cells, HYDROXYAPATITE, TISSUE engineering, NANOCOMPOSITE materials, TISSUE scaffolds
Abstract

Background: Dental tissue engineering (DTE) is an inspiring biological approach to replacing lost teeth. The strength of the differentiation of basic endometrial cells to ameloblasts is yet to be fully understood. Moreover, the construction of a scaffold, upon which ameloblasts can both grow and replicate, has been a challenge in DTE. Methods: This is an experimental study. Basic endometrial cells were first isolated and cultured before induction of differentiation to ameloblast cells by adjacency to mesenchymal rat cells and application of fibroblast growth factor 8. Hydroxyapatite nanoparticles were synthesized by freeze‑drying method. The nanocomposite collagen and hydroxyapatite scaffold were synthesized by the solvent casting method. Finally, the amyloblast cells were implanted into the hydroxyapatite and collagen nanocomposite scaffold. Results: The success of cell differentiation was confirmed through staining using specific antibodies against ameloblastin and amelogenin markers. Expression of ameloblast‑specific mRNAs, such as ameloblastins, amelogenins, and cytokeratin 14 was detected in the differentiated cells. Alizarin red staining clearly illustrates mineralization nodules in the differentiated cells. Staining against specific ameloblast markers showed that the differentiated ameloblast cells are expressed on the scaffold, unlike the control group. Conclusion: Based on the findings of this study, basic endometrial cells are capable of expressing the traits of ameloblast cells. The scaffold used in the present study conforms to most criteria used for dental tissue reconstruction and can be applied in DTE. [ABSTRACT FROM AUTHOR]

Published
2024
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38. Involvement of NRON and TUG1 long noncoding RNAs in inflammation and the pathogenesis of EAE.

Author

Kioumarsi, Emad, Kohan, Leila, Noorbakhsh, Farshid, Shirian, Sadegh, Gorji, Ali, and Zare-Chahoki, Ameneh

Subjects
LINCRNA, SPINAL cord, SUBCUTANEOUS injections, PATHOGENESIS, LABORATORY mice
Abstract

There is growing evidence that the long noncoding RNAs (lncRNAs) contribute to the pathogenesis of various neurodegenerative diseases such as multiple sclerosis (MS). The role of lncRNAs nuclear repressor of NFAT (NRON) and Taurine up-regulated 1 (TUG1) in the inflammatory processes occurring in the experimental autoimmune encephalomyelitis (EAE) model of MS is yet to be investigated. Transcript levels of NRON and TUG1 in acute and chronic phases of EAE and cultured macrophages as well as the correlation between NRON and TUG1 expression with inflammatory cytokines, were evaluated in this study. EAE experimental model was induced in female C57BL/6 mice with subcutaneous injection of MOG35–55/CFA. Mice were scored for 28 days and then sacrificed. The expression of lncRNAs TUG1 and NRON in lumbar spinal cords, activated and controlled macrophages as well as the expression of IL-1, IL-6, and CDe-3 inflammatory cytokines, were assayed by real-time RT-PCR. The lncRNAs TUG1 and NRON were significantly down-regulated in lumbar spinal cords tissues in the acute phase of EAE compared to the control group. TUG1 and NRON were significantly down-regulated in macrophages treated with 10 ng lipopolysaccharide (LPS) compared to the control macrophages. A negative correlation was identified between NRON and TUG1 expression and IL-1, IL-6, and CDe-3 inflammatory cytokines. The present study demonstrates the dysregulation of lncRNAs TUG1 and NRON in spinal cord tissue lesions of EAE and activated macrophages, pointing to their potential role in the pathogenesis of EAE. [ABSTRACT FROM AUTHOR]

Published
2024
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41. Tissue-Engineered Core–Shell Silk-Fibroin/Poly-l-Lactic Acid Nerve Guidance Conduit Containing Encapsulated Exosomes of Human Endometrial Stem Cells Promotes Peripheral Nerve Regeneration

Author

Namini, Mojdeh Salehi, primary, Ebrahimi-Barough, Somayeh, additional, Ai, Jafar, additional, Jahromi, Hossein Kargar, additional, mikaeiliagah, Elmira, additional, Azami, Mahmoud, additional, Bahrami, Naghmeh, additional, Lotfibakhshaiesh, Nasrin, additional, Saremi, Jamileh, additional, and Shirian, Sadegh, additional

Published
2023
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43. The healing effect of a mixture of Arnebia euchromaand animal fat on burn wounds in rats in comparison with sulfadiazine

Author

Dehkordi, Saied Habibian, Karimi, Iraj, Mills, Paul, and Shirian, Sadegh

Abstract

Objective:Thermal burn is a serious cause of morbidity and mortality that affects millions of people worldwide. The aim of this experimental study was to investigate the efficacy of Arnebia euchroma(AE) to treat burn wounds in a rat model.Method:A total of 80 male rats (200–250g) were shaved over the back of the neck (2×3cm2) and a second-degree burn wound was induced at this site under general anaesthesia. The rats were then randomly assigned to one of four groups (each n=20) and the burns were treated daily for 14 days as follows: (1) dressed with animal fat; (2) dressed with sulfadiazine; (3) dressed with a mixture of AE and animal fat; (4) no treatment (control). Five rats from each group were sacrificed on days 3, 5, 9 and 14 post-burn and the wounds were evaluated histologically and immunohistochemically for the expression of interleukin (IL)-1 and IL-6.Results:There was a significant increase at day 3 and decrease on day 5 samples for the expression of IL-1 in the AE plus fat group and IL-6 in the AE plus fat and sulfadiazine groups, compared to the control and fat treatment groups, respectively. Both AE plus fat and sulfadiazine treatments reduced inflammation and granulation tissue formation by day 5 post-burn, while re-epithelialisation commenced by day 9 post-burn. In addition, burns treated with AE plus fat exhibited keratinised epidermis, associated with regular collagen fibres, compared to moderately dense collagen fibres without vascularisation in the sulfadiazine group.Conclusion:These findings suggested that AE plus fat was superior to sulfadiazine in enhancing burn wound healing in rats.

Published
2024
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44. Green synthesis of copper oxide nanoparticles using Artemisia annuaaqueous extract and its characterization, antioxidant, and burn wound healing activities

Author

Karimi, Behnaz, Mardani, Masoumeh, Kaboutari, Jahangir, Javdani, Moosa, Albadi, Jalal, and Shirian, Sadegh

Abstract

The current study reports a simple and eco-friendly method for synthesis of copper oxide nanoparticles (CuO NPs) using an aqueous extract of Artemisia annua.The green synthesized CuO NPs were characterized using UV–Vis, FT-IR spectroscopy, X-ray diffraction (XRD), scanning electron microscopy, and energy dispersive X-ray analysis (EDX). The UV–Vis spectroscopy showed maximum absorption at 250 nm, and the average diameter of the biosynthesized NPs was found to be 35 nm. FTIR showed the presence of functional groups, especially C=O and O–H in bioactive constituents present that acted as reducing and capping agents during the biosynthesis of CuO NPs. The antioxidant activity of the biosynthesized CuO NPs was evaluated by DPPH assay, which showed that at 100 µg/ml, the highest antioxidant activity of 67% with an IC50 value of 73.48 µg/ml. In the in vivo experiment, after inducing 2nd degree burn wounds, the rats were randomly divided into five groups, including the untreated control, treatment groups with eucerin ointment, 0.2% CuO NPs ointment, 0.2% biosynthesized CuO NPs ointment, and 5% Artemisia annuaextract, respectively, twice a day for 21 days. Application of biosynthesized CuO NPs ointment significantly increased the levels of transforming growth factor-beta1 (TGF-β1) and hydroxyproline compared to the control. Additionally, histopathology studies showed significant progress in re-epithelization, collagen fiber deposition, and angiogenesis in the biosynthesized CuO NPs. The green synthesized CuO NPs had enhanced antioxidant, and wound healing properties and, therefore, could be considered a promising candidate in the biomedical field.

Published
2024
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46. Immunological evaluation of a novel multi-antigenic DNA vaccine encoding SAG1, SAG3, MIC4, GRA5, GRA7, AMA1and BAG1 against Toxoplasma gondii in BALB/c mice

Author

Alijani, Mohammadreza, primary, Saffar, Behnaz, additional, Yosefi Darani, Hossein, additional, Mahzounieh, Mohammadreza, additional, Fasihi- Ramandi, Mahdi, additional, Shakshi-Niaei, Mostafa, additional, Soltani, Sodabe, additional, Ghaemi, Amir, additional, and Shirian, Sadegh, additional

Published
2023
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