21 results on '"Shinta Mariana"'
Search Results
2. Nonmechanical parfocal and autofocus features based on wave propagation distribution in lensfree holographic microscopy
- Author
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Agus Budi Dharmawan, Shinta Mariana, Gregor Scholz, Philipp Hörmann, Torben Schulze, Kuwat Triyana, Mayra Garcés-Schröder, Ingo Rustenbeck, Karsten Hiller, Hutomo Suryo Wasisto, and Andreas Waag
- Subjects
Medicine ,Science - Abstract
Abstract Performing long-term cell observations is a non-trivial task for conventional optical microscopy, since it is usually not compatible with environments of an incubator and its temperature and humidity requirements. Lensless holographic microscopy, being entirely based on semiconductor chips without lenses and without any moving parts, has proven to be a very interesting alternative to conventional microscopy. Here, we report on the integration of a computational parfocal feature, which operates based on wave propagation distribution analysis, to perform a fast autofocusing process. This unique non-mechanical focusing approach was implemented to keep the imaged object staying in-focus during continuous long-term and real-time recordings. A light-emitting diode (LED) combined with pinhole setup was used to realize a point light source, leading to a resolution down to 2.76 μm. Our approach delivers not only in-focus sharp images of dynamic cells, but also three-dimensional (3D) information on their (x, y, z)-positions. System reliability tests were conducted inside a sealed incubator to monitor cultures of three different biological living cells (i.e., MIN6, neuroblastoma (SH-SY5Y), and Prorocentrum minimum). Altogether, this autofocusing framework enables new opportunities for highly integrated microscopic imaging and dynamic tracking of moving objects in harsh environments with large sample areas.
- Published
- 2021
- Full Text
- View/download PDF
3. Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy
- Author
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Gregor Scholz, Shinta Mariana, Agus Budi Dharmawan, Iqbal Syamsu, Philipp Hörmann, Carsten Reuse, Jana Hartmann, Karsten Hiller, Joan Daniel Prades, Hutomo Suryo Wasisto, and Andreas Waag
- Subjects
lensless holographic microscopy ,LED ,Complementary Metal-Oxide Semiconductor (CMOS) image sensor ,cell culture ,cell imaging ,cell counting ,Chemical technology ,TP1-1185 - Abstract
Continuous cell culture monitoring as a way of investigating growth, proliferation, and kinetics of biological experiments is in high demand. However, commercially available solutions are typically expensive and large in size. Digital inline-holographic microscopes (DIHM) can provide a cost-effective alternative to conventional microscopes, bridging the gap towards live-cell culture imaging. In this work, a DIHM is built from inexpensive components and applied to different cell cultures. The images are reconstructed by computational methods and the data are analyzed with particle detection and tracking methods. Counting of cells as well as movement tracking of living cells is demonstrated, showing the feasibility of using a field-portable DIHM for basic cell culture investigation and bringing about the potential to deeply understand cell motility.
- Published
- 2019
- Full Text
- View/download PDF
4. Continuous Live-Cell Culture Monitoring by Compact Lensless LED Microscopes
- Author
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Gregor Scholz, Shinta Mariana, Iqbal Syamsu, Agus Budi Dharmawan, Torben Schulze, Kai Mattern, Philipp Hörmann, Jana Hartmann, Andreas Dietzel, Ingo Rustenbeck, Karsten Hiller, Joan Daniel Prades, Andreas Waag, and Hutomo Suryo Wasisto
- Subjects
lensless holographic microscopy ,LED ,CMOS image sensor ,cell culture ,cell imaging ,cell counting ,General Works - Abstract
A compact lensless microscope comprising a custom-made LED engine and a CMOS imaging sensor has been developed for live-cell culture imaging inside a cell incubator environment. The imaging technique is based on digital inline-holographic microscopy, while the image reconstruction is carried out by angular spectrum approach with a custom written software. The system was tested with various biological samples including immortalized mouse astrocyte cells inside a petri dish. Besides the imaging possibility, the capability of automated cell counting and tracking could be demonstrated. By using image sensors capable of video frame rate, time series of cell movement can be captured.
- Published
- 2018
- Full Text
- View/download PDF
5. Pinhole microLED Array as Point Source Illumination for Miniaturized Lensless Cell Monitoring Systems
- Author
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Shinta Mariana, Gregor Scholz, Feng Yu, Agus Budi Dharmawan, Iqbal Syamsu, Joan Daniel Prades, Andreas Waag, and Hutomo Suryo Wasisto
- Subjects
microLED ,pinhole ,point light source ,cell monitoring ,compact lensless holographic microscope ,microfabrication ,cell imaging ,General Works - Abstract
Pinhole‐shaped light‐emitting diode (LED) arrays with dimension ranging from 100 μm down to 5 μm have been developed as point illumination sources. The proposed microLED arrays, which are based on gallium nitride (GaN) technology and emitting in the blue spectral region (λ = 465 nm), are integrated into a compact lensless holographic microscope for a non‐invasive, label‐free cell sensing and imaging. From the experimental results using single pinhole LEDs having a diameter of 90 μm, the reconstructed images display better resolution and enhanced image quality compared to those captured using a commercial surface‐mount device (SMD)‐based LED.
- Published
- 2018
- Full Text
- View/download PDF
6. Artificial Neural Networks for Automated Cell Quantification in Lensless LED Imaging Systems
- Author
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Agus Budi Dharmawan, Gregor Scholz, Shinta Mariana, Philipp Hörmann, Igi Ardiyanto, Sunu Wibirama, Jana Hartmann, Joan Daniel Prades, Karsten Hiller, Andreas Waag, and Hutomo Suryo Wasisto
- Subjects
artificial neural networks ,cell counting ,lensless holographic microscopy ,principal component analysis ,General Works - Abstract
Cell registration by artificial neural networks (ANNs) in combination with principal component analysis (PCA) has been demonstrated for cell images acquired by light emitting diode (LED)-based compact holographic microscopy. In this approach, principal component analysis was used to find the feature values from cells and background, which would be subsequently employed as neural inputs into the artificial neural networks. Image datasets were acquired from multiple cell cultures using a lensless microscope, where the reference data was generated by a manually analyzed recording. To evaluate the developed automatic cell counter, the trained system was assessed on different data sets to detect immortalized mouse astrocytes, exhibiting a detection accuracy of ~81% compared with manual analysis. The results show that the feature values from principal component analysis and feature learning by artificial neural networks are able to provide an automatic approach on the cell detection and registration in lensless holographic imaging.
- Published
- 2018
- Full Text
- View/download PDF
7. Nanofabrication of Vertically Aligned 3D GaN Nanowire Arrays with Sub-50 nm Feature Sizes Using Nanosphere Lift-off Lithography
- Author
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Tony Granz, Shinta Mariana, Gerry Hamdana, Feng Yu, Muhammad Fahlesa Fatahilah, Irene Manglano Clavero, Prabowo Puranto, Zhi Li, Uwe Brand, Joan Daniel Prades, Erwin Peiner, Andreas Waag, and Hutomo Suryo Wasisto
- Subjects
colloidal lithography ,nanosphere lithography ,nanostructure fabrication ,gallium nitride (GaN) ,ICP-DRIE ,wet chemical etching ,selective area deposition ,GaN surface treatment ,General Works - Abstract
Vertically aligned 3D gallium nitride (GaN) nanowire arrays with sub-50 nm feature sizes were fabricated using a nanosphere lift-off lithography (NSLL) technique combined with hybrid top-down etching steps (i.e., inductively coupled plasma dry reactive ion etching (ICP-DRIE) and wet chemical etching). Owing to the well-controlled chemical surface treatment prior to the nanobead deposition and etching process, vertical GaN nanowire arrays with diameter of ~35 nm, pitch of ~350 nm, and aspect ratio of >10 could be realized using 500 nm polystyrene nanobead (PN) masks. This work has demonstrated a feasibility of using NSLL as an alternative for other sophisticated but expensive nanolithography methods to manufacture low-cost but highly ordered 3D GaN nanostructures.
- Published
- 2017
- Full Text
- View/download PDF
8. Vertical 3D GaN Nanoarchitectures towards an Integrated Optoelectronic Biosensing Platform in Microbial Fuel Cells
- Author
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Heidi Boht, Hilke Wichmann, Gregor Scholz, Feng Yu, Klaas Strempel, Shinta Mariana, Muhammad Fahlesa Fatahilah, Irene Manglano Clavero, Joan Daniel Prades, Uwe Schröder, Hutomo Suryo Wasisto, and Andreas Waag
- Subjects
microbial fuel cell (MFC) ,electrochemically active biofilms ,gallium nitride ,biocompatibility ,nanowires ,optoelectronic integrated microchip ,General Works - Abstract
An integrated nano-optoelectronic biological sensor system is developed to obtain insights of the biochemical and physical processes of Geobacter sulfurreducens-based biofilm growth inside a miniaturized microbial fuel cell (MFC) reactor. Gallium nitride (GaN), which was used as a novel electrode material, has been investigated in terms of its biocompatibility and performance to transport the electrons delivered by the microorganisms. Moreover, in order to enhance the produced current density, vertical 3D GaN nanoarchitectures (i.e., arrays of nanowires and nanofins) with larger surface-to-volume ratios were fabricated using a top-down nanomachining method involving nanolithography and hybrid etching technique.
- Published
- 2017
- Full Text
- View/download PDF
9. Pectin Derived from Hydrolysis of Ripe Kepok Kuning Banana Peel Powder Employing Crude Pectinases Produced by Aspergillus niger
- Author
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Andri Cahyo Kumoro Andri Cahyo Kumoro, Shinta Mariana Shinta Mariana, Tri Hanly Maurice Tri Hanly Maurice, and Jefri Pandu Hidayat Jefri Pandu Hidayat
- Subjects
Multidisciplinary - Abstract
Banana fruits consumption generates about 35% weight of peel waste containing approximately 10.61 to 24 w/w% of pectin. Hence, improper banana peel waste management may induce various environmental and health issues. The objectives of this work were to study the effect of substrate concentration, pH, temperature, and duration on the yield of pectin extracted from enzymatic hydrolysis of banana peel powder. In this work, the crude enzymes were obtained via submerged fermentation of Kepok Kuning banana peel powder utilising Aspergillus niger and directly used without prior purification. Pectin extraction from banana peel powder was performed through hydrolysis using crude pectinases at various substrate concentrations (0.033 to 0.123 g/mL), pH (4.0 to 6.0), and temperature (40 to 70°C) for 180 min. The increase of extraction parameters enhanced the pectin yield to a maximum value and then declined. High substrate concentration, temperature, pH, and monomeric pectin compounds formation at long hydrolysis duration were found to reduce enzyme activity. A recommended extraction condition is using 0.103 g/mL substrate concentration, pH 5.0, and 55 °C for 120 min to achieve 10.80% weight yield. Commercial implementations of the results can be worthwhile in solving the environmental problem and enhance the economic value of pectin-rich fruit peels and other agricultural wastes.
- Published
- 2022
10. Wafer-scale transfer route for top–down III-nitride nanowire LED arrays based on the femtosecond laser lift-off technique
- Author
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Hutomo Suryo Wasisto, Alina Syring, Patrick Schnell, Andam Deatama Refino, Shinta Mariana, Fatwa F. Abdi, Ruri Agung Wahyuono, Winfried Daum, Florian Meierhofer, Nurhalis Majid, Kuwat Triyana, Nursidik Yulianto, Tobias Voss, and Andreas Waag
- Subjects
Technology ,Materials science ,Materials Science (miscellaneous) ,Nanowire ,Gallium nitride ,02 engineering and technology ,Nitride ,Epitaxy ,01 natural sciences ,Article ,Industrial and Manufacturing Engineering ,law.invention ,chemistry.chemical_compound ,law ,Etching (microfabrication) ,0103 physical sciences ,Veröffentlichung der TU Braunschweig ,Wafer ,Electrical and Electronic Engineering ,ddc:5 ,010302 applied physics ,business.industry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Engineering (General). Civil engineering (General) ,Atomic and Molecular Physics, and Optics ,chemistry ,ddc:53 ,Femtosecond ,Optoelectronics ,Chemical Energy Carriers ,Publikationsfonds der TU Braunschweig ,Photolithography ,TA1-2040 ,0210 nano-technology ,business - Abstract
The integration of gallium nitride (GaN) nanowire light-emitting diodes (nanoLEDs) on flexible substrates offers opportunities for applications beyond rigid solid-state lighting (e.g., for wearable optoelectronics and bendable inorganic displays). Here, we report on a fast physical transfer route based on femtosecond laser lift-off (fs-LLO) to realize wafer-scale top–down GaN nanoLED arrays on unconventional platforms. Combined with photolithography and hybrid etching processes, we successfully transferred GaN blue nanoLEDs from a full two-inch sapphire substrate onto a flexible copper (Cu) foil with a high nanowire density (~107 wires/cm2), transfer yield (~99.5%), and reproducibility. Various nanoanalytical measurements were conducted to evaluate the performance and limitations of the fs-LLO technique as well as to gain insights into physical material properties such as strain relaxation and assess the maturity of the transfer process. This work could enable the easy recycling of native growth substrates and inspire the development of large-scale hybrid GaN nanowire optoelectronic devices by solely employing standard epitaxial LED wafers (i.e., customized LED wafers with additional embedded sacrificial materials and a complicated growth process are not required).
- Published
- 2021
11. Vertical GaN Nanowires and Nanoscale Light-Emitting-Diode Arrays for Lighting and Sensing Applications
- Author
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Hendrik Spende, Joan Daniel Prades, Jan Gülink, Hutomo Suryo Wasisto, Shinta Mariana, Tony Granz, Feng Yu, Erwin Peiner, Gerry Hamdana, Andreas Waag, Nursidik Yulianto, and Klaas Strempel
- Subjects
Materials science ,business.industry ,Nanowire ,Cathodoluminescence ,Gallium nitride ,Indium gallium nitride ,Isotropic etching ,law.invention ,chemistry.chemical_compound ,chemistry ,Etching (microfabrication) ,law ,Optoelectronics ,General Materials Science ,Reactive-ion etching ,business ,Light-emitting diode - Abstract
For various lighting and monolithic sensor systems application, vertically aligned three-dimensional (3D) gallium nitride (GaN)- and indium gallium nitride (InGaN)/GaN-based LED nanowire arrays with sub-200 nm feature sizes (down to 35 nm) were fabricated using a nanosphere lift-off lithography (NSLL) technique combined with hybrid top-down etching (i.e., inductively coupled plasma dry reactive ion etching (ICP-DRIE) and wet chemical etching). Owing to the lithographic opening and well-controlled surface functionalization prior to the polystyrene nanosphere (PN) deposition, vertical GaN nanowire arrays with an area density of 9.74 × 108 cm–2 and an aspect ratio of >10 could be realized in a specified large area of 1.5 × 1.5 mm2. Optoelectrical characteristics of the nanoLEDs were further investigated in cathodoluminescence (CL) measurements, in which multiquantum well (MQW) shows a clear CL-emission at a wavelength of 465 nm. Thus, using NSLL to manufacture low-cost but highly ordered 3D GaN-based nanowir...
- Published
- 2019
12. Nonmechanical parfocal and autofocus features based on wave propagation distribution in lensfree holographic microscopy
- Author
-
Andreas Waag, Shinta Mariana, Mayra Garcés-Schröder, Karsten Hiller, Gregor Scholz, Ingo Rustenbeck, Agus Budi Dharmawan, Kuwat Triyana, Philipp Hörmann, Hutomo Suryo Wasisto, and Torben Schulze
- Subjects
0301 basic medicine ,Point light source ,Computer science ,Science ,Holography ,02 engineering and technology ,Tracking (particle physics) ,Article ,Optical imaging ,law.invention ,Interference microscopy ,03 medical and health sciences ,ddc:0 ,Optics ,Optical microscope ,law ,Microscopy ,ddc:00 ,Veröffentlichung der TU Braunschweig ,Autofocus ,Multidisciplinary ,business.industry ,Resolution (electron density) ,Imaging and sensing ,021001 nanoscience & nanotechnology ,Lens (optics) ,030104 developmental biology ,Feature (computer vision) ,Parfocal lens ,Microscopic imaging ,Medicine ,Pinhole (optics) ,ddc:004 ,Publikationsfonds der TU Braunschweig ,0210 nano-technology ,business - Abstract
Performing long-term cell observations is a non-trivial task for conventional optical microscopy, since it is usually not compatible with environments of an incubator and its temperature and humidity requirements. Lensless holographic microscopy, being entirely based on semiconductor chips without lenses and without any moving parts, has proven to be a very interesting alternative to conventional microscopy. Here, we report on the integration of a computational parfocal feature, which operates based on wave propagation distribution analysis, to perform a fast autofocusing process. This unique non-mechanical focusing approach was implemented to keep the imaged object staying in-focus during continuous long-term and real-time recordings. A light-emitting diode (LED) combined with pinhole setup was used to realize a point light source, leading to a resolution down to 2.76 μm. Our approach delivers not only in-focus sharp images of dynamic cells, but also three-dimensional (3D) information on their (x, y, z)-positions. System reliability tests were conducted inside a sealed incubator to monitor cultures of three different biological living cells (i.e., MIN6, neuroblastoma (SH-SY5Y), and Prorocentrum minimum). Altogether, this autofocusing framework enables new opportunities for highly integrated microscopic imaging and dynamic tracking of moving objects in harsh environments with large sample areas.
- Published
- 2020
13. Silicon Nanopillars with ZNO Nanorods by Nanosphere Lithography on a Piezoresistive Microcantilever
- Author
-
Maik Bertke, Changfeng Fan, Steffen Bornemann, Erwin Peiner, Angelika Schmidt, Hutomo Suryo Wasisto, Andreas Waag, Jiushuai Xu, and Shinta Mariana
- Subjects
Materials science ,Silicon ,chemistry.chemical_element ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Piezoresistive effect ,0104 chemical sciences ,chemistry ,Sputtering ,Nanosphere lithography ,Nanorod ,Dry etching ,0210 nano-technology ,Chemical bath deposition ,Nanopillar - Abstract
This paper reports on the fabrication of 3D silicon nanopillars (NPLs) modified with ZnO nanorods (NRs) (i.e., ZnO-NRs@Si-NPLs) on a piezoresistive Si microcantilever (MC) for sensing application. A monolayer-colloidal-crystal film of polystyrene (PS) nanoparticles (NPs) was deposited and used as physical mask for cryogenic Si dry etching. Vertically aligned Si-NPLs were selectively etched on the MC backside-surface. In addition, ZnO-NRs were grown in-situ on the Si NPLs using a sputtering/oxidizing and chemical bath deposition two-step method, resulting in a 3D ZnO-NRs@Si-NPLs on the MC backside.
- Published
- 2019
14. Artificial Neural Networks for Automated Cell Quantification in Lensless LED Imaging Systems
- Author
-
Andreas Waag, Karsten Hiller, Agus Budi Dharmawan, Hutomo Suryo Wasisto, Igi Ardiyanto, Jana Hartmann, Gregor Scholz, Joan Daniel Prades, Sunu Wibirama, Philipp Hörmann, and Shinta Mariana
- Subjects
Microscope ,principal component analysis ,Computer science ,Reference data (financial markets) ,Holography ,lcsh:A ,02 engineering and technology ,01 natural sciences ,law.invention ,cell counting ,law ,lensless holographic microscopy ,0103 physical sciences ,Microscopy ,010302 applied physics ,Artificial neural network ,business.industry ,Pattern recognition ,021001 nanoscience & nanotechnology ,Feature (computer vision) ,Principal component analysis ,Artificial intelligence ,lcsh:General Works ,0210 nano-technology ,business ,artificial neural networks ,Feature learning - Abstract
Cell registration by artificial neural networks (ANNs) in combination with principal component analysis (PCA) has been demonstrated for cell images acquired by light emitting diode (LED)-based compact holographic microscopy. In this approach, principal component analysis was used to find the feature values from cells and background, which would be subsequently employed as neural inputs into the artificial neural networks. Image datasets were acquired from multiple cell cultures using a lensless microscope, where the reference data was generated by a manually analyzed recording. To evaluate the developed automatic cell counter, the trained system was assessed on different data sets to detect immortalized mouse astrocytes, exhibiting a detection accuracy of ~81% compared with manual analysis. The results show that the feature values from principal component analysis and feature learning by artificial neural networks are able to provide an automatic approach on the cell detection and registration in lensless holographic imaging.
- Published
- 2018
15. Pinhole microLED Array as Point Source Illumination for Miniaturized Lensless Cell Monitoring Systems
- Author
-
Agus Budi Dharmawan, Gregor Scholz, Andreas Waag, Hutomo Suryo Wasisto, Feng Yu, Shinta Mariana, Iqbal Syamsu, and Joan Daniel Prades
- Subjects
compact lensless holographic microscope ,Materials science ,Microscope ,Image quality ,MicroLED ,Holography ,lcsh:A ,Gallium nitride ,02 engineering and technology ,law.invention ,03 medical and health sciences ,chemistry.chemical_compound ,Optics ,law ,cell imaging ,point light source ,microLED ,pinhole ,microfabrication ,030304 developmental biology ,0303 health sciences ,cell monitoring ,business.industry ,021001 nanoscience & nanotechnology ,chemistry ,Pinhole (optics) ,lcsh:General Works ,0210 nano-technology ,business ,Light-emitting diode ,Microfabrication - Abstract
Pinhole‐shaped light‐emitting diode (LED) arrays with dimension ranging from 100 μm down to 5 μm have been developed as point illumination sources. The proposed microLED arrays, which are based on gallium nitride (GaN) technology and emitting in the blue spectral region (λ = 465 nm), are integrated into a compact lensless holographic microscope for a non‐invasive, label‐free cell sensing and imaging. From the experimental results using single pinhole LEDs having a diameter of 90 μm, the reconstructed images display better resolution and enhanced image quality compared to those captured using a commercial surface‐mount device (SMD)‐based LED.
- Published
- 2018
16. Selective-area femtosecond laser lift-off processing of GaN LED chips
- Author
-
Yulianto, Nursidik, Bornemann, Steffen, Gülink, Jan, Herbani, Yuliati, Shinta Mariana, Adji, Raden Priyo Hartono, Prades, Joan Daniel, Waag, Andreas, and Wasisto, Hutomo Suryo
- Subjects
femtosecond laser ,LED ,laser lift-off ,GaN - Abstract
Gallium nitride (GaN) is a promising semiconductor material for creating versatile LED devices in various applications, including solid-state lighting, augmented reality, and sensors. By transferring the nanostructured GaN LEDs onto flexible substrates, they could even exhibit enhanced performance compared to flexible organic LEDs in terms of their flexibility, longevity, and external quantum efficiency. To release GaN LED chips from their original sapphire substrates to other substrates, laser lift-off (LLO) has been regularly utilized in production of power LEDs as a reliable and reproducible technology. For the normal LLO process, photons with energies above the band gap of GaN are used, so that the energy is dissipated at the sapphire/GaN interface. These LLO processes are based on expensive excimer laser technology, and due to limitations in available wavelengths, not transferable to AlGaN LLO with higher band gaps. In this work, a femtosecond LLO technology is proposed and demonstrated to realize free-standing GaN LED chips. The used laser has a wavelength of 520 nm, a pulse length of 350 fs, and a repetition rate of 200 kHz. Even though the impinging photons have lower energy than the GaN bandgap (3.4 eV), the directed laser pulse is transmitted through the sapphire and reaches the interface to the n-GaN layer, where it is absorbed by non-linear two-photon excitation. Consequently, the GaN is decomposed resulting in lift-off from the sapphire. The LED selective-area transfer is performed using a two-step LLO process with different laser energies (Figure 1). From the experiments, InGaN/GaN LED chips with a total area of 1 × 1 mm² were successfully separated from the sapphire substrate (Figure 2), which exhibited an emission spectrum with peak wavelengths of ~465 nm in cathodoluminescence and electroluminescence measurements. Transmission electron microscopy was also used to verify the condition of InGaN/GaN multi-quantum wells (MQWs).
- Published
- 2018
17. Continuous Live-Cell Culture Monitoring by Compact Lensless LED Microscopes
- Author
-
Karsten Hiller, Torben Schulze, Jana Hartmann, Ingo Rustenbeck, Hutomo Suryo Wasisto, Andreas Dietzel, Agus Budi Dharmawan, Philipp Hörmann, Andreas Waag, Gregor Scholz, Kai Mattern, Joan Daniel Prades, Iqbal Syamsu, and Shinta Mariana
- Subjects
Microscope ,Computer science ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,lcsh:A ,02 engineering and technology ,Iterative reconstruction ,01 natural sciences ,law.invention ,cell counting ,Software ,law ,cell imaging ,lensless holographic microscopy ,Microscopy ,Computer vision ,Image sensor ,cell culture ,business.industry ,Petri dish ,LED ,010401 analytical chemistry ,021001 nanoscience & nanotechnology ,Frame rate ,0104 chemical sciences ,Angular spectrum method ,CMOS image sensor ,Artificial intelligence ,lcsh:General Works ,0210 nano-technology ,business - Abstract
A compact lensless microscope comprising a custom-made LED engine and a CMOS imaging sensor has been developed for live-cell culture imaging inside a cell incubator environment. The imaging technique is based on digital inline-holographic microscopy, while the image reconstruction is carried out by angular spectrum approach with a custom written software. The system was tested with various biological samples including immortalized mouse astrocyte cells inside a petri dish. Besides the imaging possibility, the capability of automated cell counting and tracking could be demonstrated. By using image sensors capable of video frame rate, time series of cell movement can be captured.
- Published
- 2018
- Full Text
- View/download PDF
18. Nanofabrication of Vertically Aligned 3D GaN Nanowire Arrays with Sub-50 nm Feature Sizes Using Nanosphere Lift-off Lithography
- Author
-
Prabowo Puranto, Tony Granz, Zhi Li, Uwe Brand, Muhammad Fahlesa Fatahilah, Shinta Mariana, Irene Manglano Clavero, Hutomo Suryo Wasisto, Joan Daniel Prades, Feng Yu, Erwin Peiner, Andreas Waag, and Gerry Hamdana
- Subjects
GaN surface treatment ,Materials science ,Nanowire ,nanostructure fabrication ,lcsh:A ,Gallium nitride ,Nanotechnology ,ICP-DRIE ,Isotropic etching ,wet chemical etching ,selective area deposition ,chemistry.chemical_compound ,Nanolithography ,chemistry ,colloidal lithography ,nanosphere lithography ,Nanosphere lithography ,gallium nitride (GaN) ,lcsh:General Works ,Reactive-ion etching ,Lithography ,Next-generation lithography - Abstract
Vertically aligned 3D gallium nitride (GaN) nanowire arrays with sub-50 nm feature sizes were fabricated using a nanosphere lift-off lithography (NSLL) technique combined with hybrid top-down etching steps (i.e., inductively coupled plasma dry reactive ion etching (ICP-DRIE) and wet chemical etching). Owing to the well-controlled chemical surface treatment prior to the nanobead deposition and etching process, vertical GaN nanowire arrays with diameter of ~35 nm, pitch of ~350 nm, and aspect ratio of >10 could be realized using 500 nm polystyrene nanobead (PN) masks. This work has demonstrated a feasibility of using NSLL as an alternative for other sophisticated but expensive nanolithography methods to manufacture low-cost but highly ordered 3D GaN nanostructures.
- Published
- 2017
19. Design and fabrication of AlN-on-Si chirped surface acoustic wave resonators for label-free cell detection
- Author
-
Erwin Peiner, Shinta Mariana, Andreas Waag, Nursidik Yulianto, J. Daniel Prades, Lars Daul, Hutomo Suryo Wasisto, Iqbal Syamsu, Tony Granz, Gregor Scholz, Ludger Koenders, and Kuwat Triyana
- Subjects
History ,Fabrication ,Surface acoustic wave resonators ,Materials science ,business.industry ,Optoelectronics ,business ,Computer Science Applications ,Education ,Label free - Abstract
Chirped surface acoustic wave (SAW) resonators based on aluminum nitride (AlN) thin films have been designed and fabricated to comprehend the wave propagation characteristics induced by interdigitated transducers (IDTs) deposited on their surfaces. From the simulation results, design and geometry of the metal fingers including their width and pitch play critical roles on the wavelength of the acoustic wave and the mechanical displacement, which subsequently set the device resonant frequency. A single-step metal lift-off process involving photolithography and electron beam metal evaporation has been used to pattern and deposit Cr/Au IDT on AlN-on-Si wafers.
- Published
- 2019
20. Fabrication of SiO2 microcantilever arrays for mechanical loss measurements
- Author
-
Johannes Dickmann, Gerry Hamdana, Andreas Waag, Jan Meyer, Erwin Peiner, Gianpietro Cagnoli, Nursidik Yulianto, Maik Bertke, M. Granata, Christophe Michel, Hutomo Suryo Wasisto, Shinta Mariana, and Stefanie Kroker
- Subjects
Biomaterials ,Surface micromachining ,Oxide minerals ,Fabrication ,Materials science ,Polymers and Plastics ,business.industry ,Metals and Alloys ,Optoelectronics ,business ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Published
- 2019
21. Fabrication of SiO2 microcantilever arrays for mechanical loss measurements.
- Author
-
Shinta Mariana, Gerry Hamdana, Johannes Dickmann, Maik Bertke, Christophe Michel, Jan Meyer, Nursidik Yulianto, Gianpietro Cagnoli, Erwin Peiner, Andreas Waag, Massimo Granata, Stefanie Kroker, and Hutomo Suryo Wasisto
- Published
- 2019
- Full Text
- View/download PDF
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