Search

Your search keyword '"Shinpo, Sayaka"' showing total 11 results
Start Over Author "Shinpo, Sayaka"
11 results on '"Shinpo, Sayaka"'

3. Two Triterpene Synthases from Imperata cylindrica Catalyzing the Formation of a Pair of Diastereoisomers through Boat or Chair Cyclization

Author

Naraki, Shingo, primary, Kakihara, Mai, additional, Kato, Sayuri, additional, Saga, Yusuke, additional, Mannen, Kazuto, additional, Takase, Shohei, additional, Takano, Akihito, additional, Shinpo, Sayaka, additional, Hosouchi, Tsutomu, additional, Nakane, Takahisa, additional, Suzuki, Hideyuki, additional, and Kushiro, Tetsuo, additional

Published
2021
Full Text
View/download PDF

5. Genome structure of the legume, Lotus japonicus

Author

1000070370921, Sato, Shusei, 1000060370920, Nakamura, Yasukazu, 1000080370922, Kaneko, Takakazu, Asamizu, Erika, Kato, Tomohiko, Nakao, Mitsuteru, Sasamoto, Shigemi, Watanabe, Akiko, Ono, Akiko, Kawashima, Kumiko, Fujishiro, Tsunakazu, Katoh, Midori, Kohara, Mitsuyo, Kishida, Yoshie, Minami, Chiharu, Nakayama, Shinobu, Nakazaki, Naomi, Shimizu, Yoshimi, Shinpo, Sayaka, Takahashi, Chika, Wada, Tsuyuko, Yamada, Manabu, 1000030343263, Ohmido, Nobuko, Hayashi, Makoto, 1000000311770, Fukui, Kiichi, Baba, Tomoya, Nakamichi, Tomoko, 1000090182203, Mori, Hirotada, 1000070197549, Tabata, Satoshi, 1000070370921, Sato, Shusei, 1000060370920, Nakamura, Yasukazu, 1000080370922, Kaneko, Takakazu, Asamizu, Erika, Kato, Tomohiko, Nakao, Mitsuteru, Sasamoto, Shigemi, Watanabe, Akiko, Ono, Akiko, Kawashima, Kumiko, Fujishiro, Tsunakazu, Katoh, Midori, Kohara, Mitsuyo, Kishida, Yoshie, Minami, Chiharu, Nakayama, Shinobu, Nakazaki, Naomi, Shimizu, Yoshimi, Shinpo, Sayaka, Takahashi, Chika, Wada, Tsuyuko, Yamada, Manabu, 1000030343263, Ohmido, Nobuko, Hayashi, Makoto, 1000000311770, Fukui, Kiichi, Baba, Tomoya, Nakamichi, Tomoko, 1000090182203, Mori, Hirotada, 1000070197549, and Tabata, Satoshi

Abstract

Shusei Sato, Yasukazu Nakamura, Takakazu Kaneko, Erika Asamizu, Tomohiko Kato, Mitsuteru Nakao, Shigemi Sasamoto, Akiko Watanabe, Akiko Ono, Kumiko Kawashima, Tsunakazu Fujishiro, Midori Katoh, Mitsuyo Kohara, Yoshie Kishida, Chiharu Minami, Shinobu Nakayama, Naomi Nakazaki, Yoshimi Shimizu, Sayaka Shinpo, Chika Takahashi, Tsuyuko Wada, Manabu Yamada, Nobuko Ohmido, Makoto Hayashi, Kiichi Fukui, Tomoya Baba, Tomoko Nakamichi, Hirotada Mori, Satoshi Tabata, Genome Structure of the Legume, Lotus japonicus, DNA Research, Volume 15, Issue 4, August 2008, Pages 227–239, https://doi.org/10.1093/dnares/dsn008, The legume Lotus japonicus has been widely used as a model system to investigate the genetic background of legume-specific phenomena such as symbiotic nitrogen fixation. Here, we report structural features of the L japonicus genome. The 315.1-Mb sequences determined in this and previous studies correspond to 67% of the genome (472 Mb), and are likely to cover 91.3% of the gene space. Linkage mapping anchored 130-Mb sequences onto the six linkage groups. A total of 10 951 complete and 19 848 partial structures of protein-encoding genes were assigned to the genome. Comparative analysis of these genes revealed the expansion of several functional domains and gene families that are characteristic of L japonicus. Synteny analysis detected traces of whole-genome duplication and the presence of synteny blocks with other plant genomes to various degrees. This study provides the first opportunity to look into the complex and unique genetic system of legumes.

Published
2008

7. Construction of Signature-tagged Mutant Library in Mesorhizobium loti as a Powerful Tool for Functional Genomics.

Author

Shimoda, Yoshikazu, Mitsui, Hisayuki, Kamimatsuse, Hiroko, Minamisawa, Kiwamu, Nishiyama, Eri, Ohtsubo, Yoshiyuki, Nagata, Yuji, Tsuda, Masataka, Shinpo, Sayaka, Watanabe, Akiko, Kohara, Mitsuyo, Yamada, Manabu, Nakamura, Yasukazu, Tabata, Satoshi, and Sato, Shusei

Abstract

Rhizobia are nitrogen-fixing soil bacteria that establish endosymbiosis with some leguminous plants. The completion of several rhizobial genome sequences provides opportunities for genome-wide functional studies of the physiological roles of many rhizobial genes. In order to carry out genome-wide phenotypic screenings, we have constructed a large mutant library of the nitrogen-fixing symbiotic bacterium, Mesorhizobium loti, by transposon mutagenesis. Transposon insertion mutants were generated using the signature-tagged mutagenesis (STM) technique and a total of 29 330 independent mutants were obtained. Along with the collection of transposon mutants, we have determined the transposon insertion sites for 7892 clones, and confirmed insertions in 3680 non-redundant M. loti genes (50.5% of the total number of M. loti genes). Transposon insertions were randomly distributed throughout the M. loti genome without any bias toward G+C contents of insertion target sites and transposon plasmids used for the mutagenesis. We also show the utility of STM mutants by examining the specificity of signature tags and test screenings for growth- and nodulation-deficient mutants. This defined mutant library allows for genome-wide forward- and reverse-genetic functional studies of M. loti and will serve as an invaluable resource for researchers to further our understanding of rhizobial biology. [ABSTRACT FROM PUBLISHER]

Published
2008
Full Text
View/download PDF

8. Genome Structure of the Legume, Lotus japonicus.

Author

Sato, Shusei, Nakamura, Yasukazu, Kaneko, Takakazu, Asamizu, Erika, Kato, Tomohiko, Nakao, Mitsuteru, Sasamoto, Shigemi, Watanabe, Akiko, Ono, Akiko, Kawashima, Kumiko, Fujishiro, Tsunakazu, Katoh, Midori, Kohara, Mitsuyo, Kishida, Yoshie, Minami, Chiharu, Nakayama, Shinobu, Nakazaki, Naomi, Shimizu, Yoshimi, Shinpo, Sayaka, and Takahashi, Chika

Abstract

The legume Lotus japonicus has been widely used as a model system to investigate the genetic background of legume-specific phenomena such as symbiotic nitrogen fixation. Here, we report structural features of the L. japonicus genome. The 315.1-Mb sequences determined in this and previous studies correspond to 67% of the genome (472 Mb), and are likely to cover 91.3% of the gene space. Linkage mapping anchored 130-Mb sequences onto the six linkage groups. A total of 10 951 complete and 19 848 partial structures of protein-encoding genes were assigned to the genome. Comparative analysis of these genes revealed the expansion of several functional domains and gene families that are characteristic of L. japonicus. Synteny analysis detected traces of whole-genome duplication and the presence of synteny blocks with other plant genomes to various degrees. This study provides the first opportunity to look into the complex and unique genetic system of legumes. [ABSTRACT FROM PUBLISHER]

Published
2008
Full Text
View/download PDF

9. A Large Scale Analysis of Protein–Protein Interactions in the Nitrogen-fixing Bacterium Mesorhizobium loti.

Author

Shimoda, Yoshikazu, Shinpo, Sayaka, Kohara, Mitsuyo, Nakamura, Yasukazu, Tabata, Satoshi, and Sato, Shusei

Abstract

Global viewing of protein–protein interactions (PPIs) is a useful way to assign biological roles to large numbers of proteins predicted by complete genome sequence. Here, we systematically analyzed PPIs in the nitrogen-fixing soil bacterium Mesorhizobium loti using a modified high-throughput yeast two-hybrid system. The aims of this study are primarily on the providing functional clues to M. loti proteins that are relevant to symbiotic nitrogen fixation and conserved in other rhizobium species, especially proteins with regulatory functions and unannotated proteins. By the screening of 1542 genes as bait, 3121 independent interactions involving 1804 proteins (24% of the total protein coding genes) were identified and each interaction was evaluated using an interaction generality (IG) measure and the general features of the interacting partners. Most PPIs detected in this study are novel interactions revealing potential functional relationships between genes for symbiotic nitrogen fixation and signal transduction. Furthermore, we have predicted the putative functions of unannotated proteins through their interactions with known proteins. The results described here represent new insight into protein network of M. loti and provide useful experimental clues to elucidate the biological function of rhizobial genes that can not be assigned directly from their genomic sequence. [ABSTRACT FROM PUBLISHER]

Published
2008
Full Text
View/download PDF

10. Genome structure of the legume, Lotus japonicus

Author

Sato, Shusei, Nakamura, Yasukazu, Kaneko, Takakazu, Asamizu, Erika, Kato, Tomohiko, Nakao, Mitsuteru, Sasamoto, Shigemi, Watanabe, Akiko, Ono, Akiko, Kawashima, Kumiko, Fujishiro, Tsunakazu, Katoh, Midori, Kohara, Mitsuyo, Kishida, Yoshie, Minami, Chiharu, Nakayama, Shinobu, Nakazaki, Naomi, Shimizu, Yoshimi, Shinpo, Sayaka, Takahashi, Chika, Wada, Tsuyuko, Yamada, Manabu, Ohmido, Nobuko, Hayashi, Makoto, Fukui, Kiichi, Baba, Tomoya, Nakamichi, Tomoko, Mori, Hirotada, Tabata, Satoshi, Sato, Shusei, Nakamura, Yasukazu, Kaneko, Takakazu, Asamizu, Erika, Kato, Tomohiko, Nakao, Mitsuteru, Sasamoto, Shigemi, Watanabe, Akiko, Ono, Akiko, Kawashima, Kumiko, Fujishiro, Tsunakazu, Katoh, Midori, Kohara, Mitsuyo, Kishida, Yoshie, Minami, Chiharu, Nakayama, Shinobu, Nakazaki, Naomi, Shimizu, Yoshimi, Shinpo, Sayaka, Takahashi, Chika, Wada, Tsuyuko, Yamada, Manabu, Ohmido, Nobuko, Hayashi, Makoto, Fukui, Kiichi, Baba, Tomoya, Nakamichi, Tomoko, Mori, Hirotada, and Tabata, Satoshi

Abstract

Shusei Sato, Yasukazu Nakamura, Takakazu Kaneko, Erika Asamizu, Tomohiko Kato, Mitsuteru Nakao, Shigemi Sasamoto, Akiko Watanabe, Akiko Ono, Kumiko Kawashima, Tsunakazu Fujishiro, Midori Katoh, Mitsuyo Kohara, Yoshie Kishida, Chiharu Minami, Shinobu Nakayama, Naomi Nakazaki, Yoshimi Shimizu, Sayaka Shinpo, Chika Takahashi, Tsuyuko Wada, Manabu Yamada, Nobuko Ohmido, Makoto Hayashi, Kiichi Fukui, Tomoya Baba, Tomoko Nakamichi, Hirotada Mori, Satoshi Tabata, Genome Structure of the Legume, Lotus japonicus, DNA Research, Volume 15, Issue 4, August 2008, Pages 227–239, https://doi.org/10.1093/dnares/dsn008, The legume Lotus japonicus has been widely used as a model system to investigate the genetic background of legume-specific phenomena such as symbiotic nitrogen fixation. Here, we report structural features of the L japonicus genome. The 315.1-Mb sequences determined in this and previous studies correspond to 67% of the genome (472 Mb), and are likely to cover 91.3% of the gene space. Linkage mapping anchored 130-Mb sequences onto the six linkage groups. A total of 10 951 complete and 19 848 partial structures of protein-encoding genes were assigned to the genome. Comparative analysis of these genes revealed the expansion of several functional domains and gene families that are characteristic of L japonicus. Synteny analysis detected traces of whole-genome duplication and the presence of synteny blocks with other plant genomes to various degrees. This study provides the first opportunity to look into the complex and unique genetic system of legumes.

11. Genome structure of the legume, Lotus japonicus

Author

Sato, Shusei, Nakamura, Yasukazu, Kaneko, Takakazu, Asamizu, Erika, Kato, Tomohiko, Nakao, Mitsuteru, Sasamoto, Shigemi, Watanabe, Akiko, Ono, Akiko, Kawashima, Kumiko, Fujishiro, Tsunakazu, Katoh, Midori, Kohara, Mitsuyo, Kishida, Yoshie, Minami, Chiharu, Nakayama, Shinobu, Nakazaki, Naomi, Shimizu, Yoshimi, Shinpo, Sayaka, Takahashi, Chika, Wada, Tsuyuko, Yamada, Manabu, Ohmido, Nobuko, Hayashi, Makoto, Fukui, Kiichi, Baba, Tomoya, Nakamichi, Tomoko, Mori, Hirotada, Tabata, Satoshi, Sato, Shusei, Nakamura, Yasukazu, Kaneko, Takakazu, Asamizu, Erika, Kato, Tomohiko, Nakao, Mitsuteru, Sasamoto, Shigemi, Watanabe, Akiko, Ono, Akiko, Kawashima, Kumiko, Fujishiro, Tsunakazu, Katoh, Midori, Kohara, Mitsuyo, Kishida, Yoshie, Minami, Chiharu, Nakayama, Shinobu, Nakazaki, Naomi, Shimizu, Yoshimi, Shinpo, Sayaka, Takahashi, Chika, Wada, Tsuyuko, Yamada, Manabu, Ohmido, Nobuko, Hayashi, Makoto, Fukui, Kiichi, Baba, Tomoya, Nakamichi, Tomoko, Mori, Hirotada, and Tabata, Satoshi

Abstract

Shusei Sato, Yasukazu Nakamura, Takakazu Kaneko, Erika Asamizu, Tomohiko Kato, Mitsuteru Nakao, Shigemi Sasamoto, Akiko Watanabe, Akiko Ono, Kumiko Kawashima, Tsunakazu Fujishiro, Midori Katoh, Mitsuyo Kohara, Yoshie Kishida, Chiharu Minami, Shinobu Nakayama, Naomi Nakazaki, Yoshimi Shimizu, Sayaka Shinpo, Chika Takahashi, Tsuyuko Wada, Manabu Yamada, Nobuko Ohmido, Makoto Hayashi, Kiichi Fukui, Tomoya Baba, Tomoko Nakamichi, Hirotada Mori, Satoshi Tabata, Genome Structure of the Legume, Lotus japonicus, DNA Research, Volume 15, Issue 4, August 2008, Pages 227–239, https://doi.org/10.1093/dnares/dsn008, The legume Lotus japonicus has been widely used as a model system to investigate the genetic background of legume-specific phenomena such as symbiotic nitrogen fixation. Here, we report structural features of the L japonicus genome. The 315.1-Mb sequences determined in this and previous studies correspond to 67% of the genome (472 Mb), and are likely to cover 91.3% of the gene space. Linkage mapping anchored 130-Mb sequences onto the six linkage groups. A total of 10 951 complete and 19 848 partial structures of protein-encoding genes were assigned to the genome. Comparative analysis of these genes revealed the expansion of several functional domains and gene families that are characteristic of L japonicus. Synteny analysis detected traces of whole-genome duplication and the presence of synteny blocks with other plant genomes to various degrees. This study provides the first opportunity to look into the complex and unique genetic system of legumes.

Catalog

Books, media, physical & digital resources
See catalog results