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140 results on '"Shinde, Sudhirkumar"'

9. Sialic Acid as a Biomarker Studied in Breast Cancer Cell Lines In Vitro Using Fluorescent Molecularly Imprinted Polymers

Author

El-Schich, Zahra, Zhang, Yuecheng, Göransson, Tommy, Dizeyi, Nishtman, Persson, Jenny L., Johansson, Emil, Caraballo, Remi, Elofsson, Mikael, Shinde, Sudhirkumar, Sellergren, Börje, and Wingren, Anette Gjörloff

Subjects
lcsh:T, Cell- och molekylärbiologi, lcsh:Technology, Sialic acid, lcsh:QC1-999, Analytical Chemistry, lcsh:Chemistry, Breast cancer, breast cancer, Epithelial cell adhesion molecule, lcsh:Biology (General), lcsh:QD1-999, sialic acid, lcsh:TA1-2040, Molecularly imprinted polymers, Analytisk kemi, Nanoparticles, molecularly imprinted polymers, nanoparticles, epithelial cell adhesion molecule, lcsh:Engineering (General). Civil engineering (General), lcsh:QH301-705.5, Cell and Molecular Biology, lcsh:Physics
Abstract

Sialylations are post-translational modifications of proteins and lipids that play important roles in many cellular events, including cell-cell interactions, proliferation, and migration. Tumor cells express high levels of sialic acid (SA), which are often associated with the increased invasive potential in clinical tumors, correlating with poor prognosis. To overcome the lack of natural SA-receptors, such as antibodies and lectins with high enough specificity and sensitivity, we have used molecularly imprinted polymers (MIPs), or “plastic antibodies”, as nanoprobes. Because high expression of epithelial cell adhesion molecule (EpCAM) in primary tumors is often associated with proliferation and a more aggressive phenotype, the expression of EpCAM and CD44 was initially analyzed. The SA-MIPs were used for the detection of SA on the cell surface of breast cancer cells. Lectins that specifically bind to the a-2,3 SA and a-2,6 SA variants were used for analysis of SA expression, with both flow cytometry and confocal microscopy. Here we show a correlation of EpCAM and SA expression when using the SA-MIPs for detection of SA. We also demonstrate the binding pattern of the SA-MIPs on the breast cancer cell lines using confocal microscopy. Pre-incubation of the SA-MIPs with SA-derivatives as inhibitors could reduce the binding of the SA-MIPs to the tumor cells, indicating the specificity of the SA-MIPs. In conclusion, the SA-MIPs may be a new powerful tool in the diagnostic analysis of breast cancer cells.

Published
2021

12. Highly Efficient Synthesis and Assay of Protein‐Imprinted Nanogels by Using Magnetic Templates

Author

Mahajan, Rashmi, Rouhi, Mona, Shinde, Sudhirkumar, Bedwell, Thomas, Incel, Anil, Mavliutova, Liliia, Piletsky, Sergey, Nicholls, Ian A., and Sellergren, Börje

Subjects
plastic antibodies, Swine, Communication, Acrylic Resins, competitive assays, Nanogels, Enzymes, Immobilized, Communications, Pepsin A, Polymerization, Molecular Imprinting, Molecularly Imprinted Polymers, nanogel synthesis, Animals, Cattle, Trypsin, Magnetite Nanoparticles, Amination
Abstract

We report an approach integrating the synthesis of protein‐imprinted nanogels (“plastic antibodies”) with a highly sensitive assay employing templates attached to magnetic carriers. The enzymes trypsin and pepsin were immobilized on amino‐functionalized solgel‐coated magnetic nanoparticles (magNPs). Lightly crosslinked fluorescently doped polyacrylamide nanogels were subsequently produced by high‐dilution polymerization of monomers in the presence of the magNPs. The nanogels were characterised by a novel competitive fluorescence assay employing identical protein‐conjugated nanoparticles as ligands to reversibly immobilize the corresponding nanogels. Both nanogels exhibited K d

Published
2018

17. Sialic acid as a biomarker studied in breast cancer cells using fluorescent molecularly imprinted polymers, MIPs

Author

El-Schich, Zahra, primary, Zhang, Yuecheng, additional, Göransson, Tommy, additional, Dizeyi, Nishtman, additional, Persson, Jenny, additional, Johansson, Emil, additional, Caraballo, Remi, additional, Elofsson, Mikael, additional, Shinde, Sudhirkumar, additional, Sellergren, Börje, additional, and Wingren, Anette Gjörloff, additional

Published
2020
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22. Hierarchically templated beads with tailored pore structure for phosphopeptide capture and phosphoproteomics

Author

Wierzbicka, Celina, Torsetnes, Silje B., Jensen, Ole N., Shinde, Sudhirkumar, and Sellergren, Börje

Subjects
Chemistry, Multidisciplinary, Naturvetenskap, Natural Sciences
Abstract

Two templating approaches to produce imprinted phosphotyrosine capture beads with a controllable pore structure are reported and compared with respect to their ability to enrich phosphopeptides from a tryptic peptide mixture. The beads were prepared by the polymerization of urea-based host monomers and crosslinkers inside the pores of macroporous silica beads with both free and immobilized template. In the final step the silica was removed by fluoride etching resulting in mesoporous polymer replicas with narrow pore size distributions, pore diameters ≈ 10 nm and surface area > 260 m2 g-1. The beads displayed pronounced phosphotyrosine affinity and selectivity in binding tests using model peptides in acetonitrile rich solutions with a performance surpassing solution polymerized bulk imprinted materials. Tests of the beads for the enrichment of phosphopeptides from tryptic digests of twelve proteins revealed both pY/pS and pY/Y selectivity. This was reflected in a nearly 6-fold increase in the enrichment factor of a 23-mer pY-peptide and pY/pS normalized intensity ratios up to 1.5, when comparing the template mesoporous beads with the bulk materials.

Published
2017

23. Phosphotyrosine Biased Enrichment of Tryptic Peptides from Cancer Cells by Combining pY-MIP and TiO2Affinity Resins

Author

Bllaci, Loreta, Torsetnes, Silje B., Wierzbicka, Celina, Shinde, Sudhirkumar, Sellergren, Börje, Rogowska-Wrzesinska, Adelina, and Jensen, Ole N.

Abstract

Protein phosphorylation at distinct tyrosine residues (pY) is essential for fast, specific, and accurate signal transduction in cells. Enrichment of pY-containing peptides derived from phosphoproteins is commonly facilitated by use of immobilized anti-pY antibodies prior to phosphoproteomics analysis by mass spectrometry. We here report on an alternative approach for pY-peptide enrichment using inexpensive pY-imprinted polymer (pY-MIP). We assessed by mass spectrometry the performance of pY-MIP for enrichment and sequencing of phosphopeptides obtained by tryptic digestion of protein extracts from HeLa cells. The combination of pY-MIP- and TiO2-based phosphopeptide enrichment provided more than 90% selectivity for phosphopeptides. Mass spectrometry signal intensities were enhanced for most pY-phosphopeptides (approximately 70%) when using the pY-MIP-TiO2combination as compared to TiO2alone. pY constituted up to 8% of the pY-MIP-TiO2-enriched phosphopeptide fractions. The pY-MIP-TiO2and the TiO2protocols yielded comparable numbers of distinct phosphopeptides, 1693 and 1842, respectively, from microgram levels of peptide samples. Detailed analysis of physicochemical properties of pY-MIP-TiO2-enriched phosphopeptides demonstrated that this protocol retrieved phosphopeptides that tend to be smaller (<24 residues), less acidic, and almost exclusively monophosphorylated, as compared to TiO2alone. These unique properties render the pY-MIP-based phosphopeptide enrichment technique an attractive alternative for applications in phosphoproteomics research.

Published
2024
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35. Highly Efficient Synthesis and Assay of Protein‐Imprinted Nanogels by Using Magnetic Templates.

Author

Mahajan, Rashmi, Rouhi, Mona, Shinde, Sudhirkumar, Bedwell, Thomas, Incel, Anil, Mavliutova, Liliia, Piletsky, Sergey, Nicholls, Ian A., and Sellergren, Börje

Subjects
NANOGELS, MAGNETIC nanoparticles, IMMUNOGLOBULINS, POLYACRYLAMIDE, LIGANDS (Chemistry)
Abstract

We report an approach integrating the synthesis of protein‐imprinted nanogels ("plastic antibodies") with a highly sensitive assay employing templates attached to magnetic carriers. The enzymes trypsin and pepsin were immobilized on amino‐functionalized solgel‐coated magnetic nanoparticles (magNPs). Lightly crosslinked fluorescently doped polyacrylamide nanogels were subsequently produced by high‐dilution polymerization of monomers in the presence of the magNPs. The nanogels were characterised by a novel competitive fluorescence assay employing identical protein‐conjugated nanoparticles as ligands to reversibly immobilize the corresponding nanogels. Both nanogels exhibited Kd<10 pM for their respective target protein and low cross‐reactivity with five reference proteins. This agrees with affinities reported for solid‐phase‐synthesized nanogels prepared using low‐surface‐area glass‐bead supports. This approach simplifies the development and production of plastic antibodies and offers direct access to a practical bioassay. Magnetic imprints: A generic integrated approach for high‐yielding "plastic antibody" synthesis coupled with an ultrasensitive displacement assay format is reported. Both techniques rely on the use of template‐modified magnetic carrier particles with a high surface‐to‐volume ratio. [ABSTRACT FROM AUTHOR]

Published
2019
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41. Low-bias phosphopeptide enrichment from scarce samples using plastic antibodies

Author

Chen, Jing, primary, Shinde, Sudhirkumar, additional, Koch, Markus-Hermann, additional, Eisenacher, Martin, additional, Galozzi, Sara, additional, Lerari, Thilo, additional, Barkovits, Katalin, additional, Subedi, Prabal, additional, Krüger, Rejko, additional, Kuhlmann, Katja, additional, Sellergren, Börje, additional, Helling, Stefan, additional, and Marcus, Katrin, additional

Published
2015
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46. A Phosphotyrosine‐Imprinted Polymer Receptor for the Recognition of Tyrosine Phosphorylated Peptides

Author

Emgenbroich, Marco, primary, Borrelli, Cristiana, additional, Shinde, Sudhirkumar, additional, Lazraq, Issam, additional, Vilela, Filipe, additional, Hall, Andrew J., additional, Oxelbark, Joakim, additional, De Lorenzi, Ersilia, additional, Courtois, Julien, additional, Simanova, Anna, additional, Verhage, Jeroen, additional, Irgum, Knut, additional, Karim, Kal, additional, and Sellergren, Börje, additional

Published
2008
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47. Molecularly Imprinted Polymers for the Removal of Antide-Pressants from Contaminated Wastewater.

Author

Gornik, Tjasa, Shinde, Sudhirkumar, Lamovsek, Lea, Koblar, Maja, Heath, Ester, Sellergren, Börje, Kosjek, Tina, and Otero, Marta

Subjects
*IMPRINTED polymers, *SEROTONIN uptake inhibitors, *WASTEWATER treatment, *WATER purification, *ACTIVATED carbon, *SEWAGE
Abstract

Selective serotonin reuptake inhibitors (SSRIs) are a class of antidepressants regularly detected in the environment. This indicates that the existing wastewater treatment techniques are not successfully removing them beforehand. This study investigated the potential of molecularly imprinted polymers (MIPs) to serve as sorbents for removal of SSRIs in water treatment. Sertraline was chosen as the template for imprinting. We optimized the composition of MIPs in order to obtain materials with highest capacity, affinity, and selectivity for sertraline. We report the maximum capacity of MIP for sertraline in water at 72.6 mg g−1, and the maximum imprinting factor at 3.7. The MIPs were cross-reactive towards other SSRIs and the metabolite norsertraline. They showed a stable performance in wastewater-relevant pH range between 6 and 8, and were reusable after a short washing cycle. Despite having a smaller surface area between 27.4 and 193.8 m2·g−1, as compared to that of the activated carbon at 1400 m2·g−1, their sorption capabilities in wastewaters were generally superior. The MIPs with higher surface area and pore volume that formed more non-specific interactions with the targets considerably contributed to the overall removal efficiency, which made them better suited for use in wastewater treatment. [ABSTRACT FROM AUTHOR]

Published
2021
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48. Molecularly Imprinted Polymers for the Removal of Antidepressants from Contaminated Wastewater.

Author

Gornik T, Shinde S, Lamovsek L, Koblar M, Heath E, Sellergren B, and Kosjek T

Abstract

Selective serotonin reuptake inhibitors (SSRIs) are a class of antidepressants regularly detected in the environment. This indicates that the existing wastewater treatment techniques are not successfully removing them beforehand. This study investigated the potential of molecularly imprinted polymers (MIPs) to serve as sorbents for removal of SSRIs in water treatment. Sertraline was chosen as the template for imprinting. We optimized the composition of MIPs in order to obtain materials with highest capacity, affinity, and selectivity for sertraline. We report the maximum capacity of MIP for sertraline in water at 72.6 mg g -1 , and the maximum imprinting factor at 3.7. The MIPs were cross-reactive towards other SSRIs and the metabolite norsertraline. They showed a stable performance in wastewater-relevant pH range between 6 and 8, and were reusable after a short washing cycle. Despite having a smaller surface area between 27.4 and 193.8 m 2 ·g -1 , as compared to that of the activated carbon at 1400 m 2 ·g -1 , their sorption capabilities in wastewaters were generally superior. The MIPs with higher surface area and pore volume that formed more non-specific interactions with the targets considerably contributed to the overall removal efficiency, which made them better suited for use in wastewater treatment., Competing Interests: The authors declare no conflict of interest.

Published
2020
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49. Highly Efficient Synthesis and Assay of Protein-Imprinted Nanogels by Using Magnetic Templates.

Author

Mahajan R, Rouhi M, Shinde S, Bedwell T, Incel A, Mavliutova L, Piletsky S, Nicholls IA, and Sellergren B

Subjects
Acrylic Resins chemical synthesis, Amination, Animals, Cattle, Enzymes, Immobilized chemistry, Molecular Imprinting, Polymerization, Swine, Acrylic Resins chemistry, Magnetite Nanoparticles chemistry, Nanogels chemistry, Pepsin A chemistry, Trypsin chemistry
Abstract

We report an approach integrating the synthesis of protein-imprinted nanogels ("plastic antibodies") with a highly sensitive assay employing templates attached to magnetic carriers. The enzymes trypsin and pepsin were immobilized on amino-functionalized solgel-coated magnetic nanoparticles (magNPs). Lightly crosslinked fluorescently doped polyacrylamide nanogels were subsequently produced by high-dilution polymerization of monomers in the presence of the magNPs. The nanogels were characterised by a novel competitive fluorescence assay employing identical protein-conjugated nanoparticles as ligands to reversibly immobilize the corresponding nanogels. Both nanogels exhibited K d <10 pM for their respective target protein and low cross-reactivity with five reference proteins. This agrees with affinities reported for solid-phase-synthesized nanogels prepared using low-surface-area glass-bead supports. This approach simplifies the development and production of plastic antibodies and offers direct access to a practical bioassay., (© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Published
2019
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50. An Epitope-Imprinted Biointerface with Dynamic Bioactivity for Modulating Cell-Biomaterial Interactions.

Author

Pan G, Shinde S, Yeung SY, Jakštaitė M, Li Q, Wingren AG, and Sellergren B

Subjects
3T3 Cells, Animals, Cell Communication, Ligands, Mice, Molecular Structure, Surface Properties, Biocompatible Materials chemistry, Epitopes chemistry, Fibroblasts chemistry, Molecular Imprinting, Oligopeptides chemistry
Abstract

In this study, an epitope-imprinting strategy was employed for the dynamic display of bioactive ligands on a material interface. An imprinted surface was initially designed to exhibit specific affinity towards a short peptide (i.e., the epitope). This surface was subsequently used to anchor an epitope-tagged cell-adhesive peptide ligand (RGD: Arg-Gly-Asp). Owing to reversible epitope-binding affinity, ligand presentation and thereby cell adhesion could be controlled. As compared to current strategies for the fabrication of dynamic biointerfaces, for example, through reversible covalent or host-guest interactions, such a molecularly tunable dynamic system based on a surface-imprinting process may unlock new applications in in situ cell biology, diagnostics, and regenerative medicine., (© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Published
2017
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