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22. Evaluation of Methylene Blue Migration from Time—Temperature Indicators Using LC-MS/MS.

Author

Lee, Seung-Eun, Lim, Kyung-Jik, Lee, Yoon-Hee, and Shin, Han-Seung

Subjects
TEMPERATURE measuring instruments, LIQUID chromatography-mass spectrometry, FOOD safety, SAFETY standards, FOOD packaging, FOOD composition, PESTICIDE residues in food, PACKAGING recycling, METHYLENE blue
Abstract

The purpose of this study was to evaluate and validate methylene blue migration from printed time–temperature indicators (TTIs) into food. It also highlights the importance of establishing regulatory measures and safety standards for food packaging, suggesting that this can contribute to improving food packaging safety. Liquid chromatography–mass spectrometry (LC-MS/MS) was used to quantify methylene blue migration in various food simulant and food matrix samples. The results show that the level of methylene blue migration varies significantly depending on the chemical properties of the food mimetic and the composition of the food matrix. The established method demonstrated a high sensitivity, with limits of detection (LODs) of 0.0019–0.0706 μg/L (kg) and limits of quantification (LOQs) of 0.0057–0.2138 μg/L (kg). This study highlights the need for a regulatory framework to mitigate the health risks associated with methylene blue in intelligent packaging systems and argues that regulatory thresholds should be set to ensure food safety and quality. [ABSTRACT FROM AUTHOR]

Published
2024
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48. Synthesis of Biogenic Gold Nanoparticles by Using Sericin Protein from Bombyx mori Silk Cocoon and Investigation of Its Wound Healing, Antioxidant, and Antibacterial Potentials

Author

Das,Gitishree, Seo,Sujin, Yang,In-Jun, Nguyen,Ly Thi Huong, Shin,Han-Seung, Patra,Jayanta Kumar, Das,Gitishree, Seo,Sujin, Yang,In-Jun, Nguyen,Ly Thi Huong, Shin,Han-Seung, and Patra,Jayanta Kumar

Abstract

Gitishree Das,1 Sujin Seo,2 In-Jun Yang,3 Ly Thi Huong Nguyen,3 Han-Seung Shin,2 Jayanta Kumar Patra1 1Research Institute of Integrative Life Sciences, Dongguk University-Seoul, Goyangsi, Republic of Korea; 2Department of Food Science & Biotechnology, Dongguk University-Seoul, Goyangsi, Republic of Korea; 3Department of Physiology, College of Korean Medicine, Dongguk University, Gyeongju, Republic of KoreaCorrespondence: Jayanta Kumar Patra, Research Institute of Integrative Life Sciences, Dongguk University-Seoul, Goyangsi, 10326, Republic of Korea, Tel +82-31-961-5625, Email jkpatra@dongguk.eduIntroduction: A number of biological wastes and factory waste materials have been tested recently for the eco-friendly biosynthesis of nanoparticles. Sericin protein (SSP) is usually removed from the silk cocoon during the degumming process in the process of making the silk, and this sericin protein is normally thrown away by the sericulture industries as waste materials. It is found that this sericin protein possesses a number of biological properties.Methods: Considering this, in the present study, an effort has been made to biosynthesize gold nanoparticles (SSP-AuNPs) using the waste sericin solution as the reducing and capping agent and investigate its biopotential in terms of its wound healing, antioxidant and antibacterial activities.Results: The synthesis of SSP-AuNPs was perceived by the visual color change and confirmed by UV-Vis spectroscopy with absorption maxima at 522 nm. Further characterization of SSP-AuNPs was done by TEM, EDS, XRD, FTIR, DLS, zeta potential, TGA, AFM, etc. The size of SSP-AuNPs was found out to be 54.82 nm as per the particle size analyzer and the zeta potential is − 19.8 mV. The SSP-AuNPs displayed promising wound healing potential of 70.96 and 69.76% wound closure rate at 5 and 10 μg/mL respectively as compared to 74.91% by the Centella asiatica taken as a positive control. It also exhibited promising antioxidant p

Published
2023

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