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590 results on '"Shimotohno, K"'

1. Systems to Culture Hepatitis C Virus

Author

Kato, N., Shimotohno, K., Compans, R. W., editor, Cooper, M., editor, Hogle, J. M., editor, Ito, Y., editor, Koprowski, H., editor, Melchers, F., editor, Oldstone, M., editor, Olsnes, S., editor, Potter, M., editor, Saedler, H., editor, Vogt, P. K., editor, Wagner, H., editor, Hagedorn, Curt H., editor, and Rice, Charles M., editor

Published
2000
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9. Caspase-mediated cleavage of eukaryotic translation initiation factor subunit 2alpha

Author

Satoh, S, Hijikata, M, Handa, H, and Shimotohno, K

Subjects
Caspase 3, Tumor Necrosis Factor-alpha, Recombinant Fusion Proteins, Eukaryotic Initiation Factor-2, food and beverages, Apoptosis, environment and public health, Caspase Inhibitors, enzymes and coenzymes (carbohydrates), Kinetics, Phosphoserine, eIF-2 Kinase, Poly I-C, Caspases, Protein Biosynthesis, Mutagenesis, Site-Directed, Tumor Cells, Cultured, Humans, Cycloheximide, Phosphorylation, Poly(ADP-ribose) Polymerases, Research Article, Sequence Deletion
Abstract

Eukaryotic translation initiation factor 2alpha (eIF-2alpha), a target molecule of the interferon-inducible double-stranded-RNA-dependent protein kinase (PKR), was cleaved in apoptotic Saos-2 cells on treatment with poly(I).poly(C) or tumour necrosis factor alpha. This cleavage occurred with a time course similar to that of poly(ADP-ribose) polymerase, a well-known caspase substrate. In addition, eIF-2alpha was cleaved by recombinant active caspase-3 in vitro. By site-directed mutagenesis, the cleavage site was mapped to an Ala-Glu-Val-Asp(300) downward arrowGly(301) sequence located in the C-terminal portion of eIF-2alpha. PKR phosphorylates eIF-2alpha on Ser(51), resulting in the suppression of protein synthesis. PKR-mediated translational suppression was repressed when the C-terminally cleaved product of eIF-2alpha was overexpressed in Saos-2 cells, even though PKR can phosphorylate this cleaved product. These results suggest that caspase-3 or related protease(s) can modulate the efficiency of protein synthesis by cleaving the alpha subunit of eIF-2, a key component in the initiation of translation.

Published
1999

10. Src-homology domain 2 is responsible for transcriptional suppression induced by expression of Lck

Author

Ohta M, Nagai H, Hiroshi Nyunoya, and Shimotohno K

Subjects
Base Sequence, Transcription, Genetic, Molecular Sequence Data, Forkhead Transcription Factors, Transfection, DNA-Binding Proteins, src Homology Domains, Mutagenesis, Insertional, src-Family Kinases, Genes, Reporter, Lymphocyte Specific Protein Tyrosine Kinase p56(lck), Humans, Amino Acid Sequence, Phosphorylation, Sequence Deletion, Transcription Factors
Abstract

Overexpression of Lck was shown, by our previous study, to suppress gene transcription from various viral and cellular promoters. The suppression of transcription from human T-cell leukemia virus promoter by Lck was independent of the presence of the enhancer core sequences within the long terminal repeat. The suppression of transcription was observed with Lck mutants that had either diminished or enhanced tyrosine-kinase activity. A mutant lacking the myristylation site also suppressed transcription. From the analysis with various deletion mutants of Lck, it was suggested that Src-homology domain 2 (SH2) is both necessary and sufficient for the suppression of transcription. A similar effect was also observed with the SH2 domain of the v-src gene. Thus, overexpression of Lck could suppress gene expression through a unique function of the SH2 domain.

Published
1996

11. A PROPOSED SYSTEM FOR THE NOMENCLATURE OF HEPATITIS-C VIRAL GENOTYPES

Author

SIMMONDS, P ALBERTI, A ALTER, HJ BONINO, F BRADLEY, DW and BRECHOT, C BROUWER, JT CHAN, SW CHAYAMA, K CHEN, DS and CHOO, QL COLOMBO, M CUYPERS, HTM DATE, T DUSHEIKO, GM ESTEBAN, JI FAY, O HADZIYANNIS, SJ HAN, J and HATZAKIS, A HOLMES, EC HOTTA, H HOUGHTON, M IRVINE, B and KOHARA, M KOLBERG, JA KUO, G LAU, JYN LELIE, PN and MAERTENS, G MCOMISH, F MIYAMURA, T MIZOKAMI, M NOMOTO, A and PRINCE, AM REESINK, HW RICE, C ROGGENDORF, M SCHALM, SW SHIKATA, T SHIMOTOHNO, K STUYVER, L TREPO, C and WEINER, A YAP, PL URDEA, MS

Published
1994

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