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19 results on '"Shimizu, Toshiyasu"'

18. Induction of Thymus-Derived ?d T Cells by Escherichia coliEnterotoxin B Subunit in Peritoneal Cavities of Mice

Author

Shimizu, Toshiyasu, Sasaki, Keiko, Kato, Michio, Arimitsu, Hideyuki, Ochi, Sadayuki, Shigemori, Naoko, Wasito, Eddy Bagus, Yokochi, Takashi, and Tsuji, Takao

Abstract

ABSTRACTWe examined the activation of intraperitoneal T cells in BALB/c mice by the Escherichia colienterotoxin B subunit, which induced a specific Th2 type of T-cell response to intraperitoneally coadministered bovine immunoglobulin G. The numbers of both ?d and aß T cells increased significantly after intraperitoneal administration of the B subunit in a time-dependent manner; these numbers were not affected by the B-subunit G33D mutant, which is defective in GM1ganglioside-binding ability. Early after administration a small number of ?d T cells produced either interleukin-4 (IL-4) or gamma interferon, while late after administration primarily IL-10-producing ?d T cells were detected. ?d T cells induced by the B subunit did not express a characteristic V gene over the time course of the study. The induction of ?d T cells did not occur in athymic nu/numice but could be induced upon transplantation of fetal AKR thymus-like aß T cells. ?d T cells in athymic nu/numice with a fetal thymic graft predominantly expressed the donor Thy-1.1 antigen but not the host Thy-1.2 antigen. The induction of these T cells, however, could not be restored by coadministration of the B subunit with peritoneal cells from normal mice. These results suggest that the B subunit activates intraperitoneal ?d and aß T cells in a manner dependent upon its ability to bind to GM1ganglioside. ?d T cells induced by the B subunit are Th2-type cells derived from the thymus. These ?d T cells may be functionally involved in specific Th2 responses to the B subunit, which possibly acts as an adjuvant through the influence of aß T cells.

Published
2005
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19. Induction of thymus-derived gammadelta T Cells by Escherichia coli enterotoxin b subunit in peritoneal cavities of mice.

Author

Shimizu T, Sasaki K, Kato M, Arimitsu H, Ochi S, Shigemori N, Wasito EB, Yokochi T, and Tsuji T

Subjects
Animals, Cytokines biosynthesis, Cytokines immunology, Enterotoxins genetics, Female, G(M1) Ganglioside immunology, G(M1) Ganglioside metabolism, Immunoglobulin G immunology, Immunoglobulin Variable Region genetics, Lymphocyte Activation immunology, Mice, Receptors, Antigen, T-Cell, alpha-beta, Receptors, Antigen, T-Cell, gamma-delta, Reverse Transcriptase Polymerase Chain Reaction, T-Lymphocyte Subsets cytology, Th2 Cells cytology, Time Factors, Enterotoxins immunology, Escherichia coli immunology, Peritoneal Cavity cytology, T-Lymphocyte Subsets immunology, Th2 Cells immunology, Thymus Gland cytology
Abstract

We examined the activation of intraperitoneal T cells in BALB/c mice by the Escherichia coli enterotoxin B subunit, which induced a specific Th2 type of T-cell response to intraperitoneally coadministered bovine immunoglobulin G. The numbers of both gammadelta and alphabeta T cells increased significantly after intraperitoneal administration of the B subunit in a time-dependent manner; these numbers were not affected by the B-subunit G33D mutant, which is defective in GM1 ganglioside-binding ability. Early after administration a small number of gammadelta T cells produced either interleukin-4 (IL-4) or gamma interferon, while late after administration primarily IL-10-producing gammadelta T cells were detected. gammadelta T cells induced by the B subunit did not express a characteristic V gene over the time course of the study. The induction of gammadelta T cells did not occur in athymic nu/nu mice but could be induced upon transplantation of fetal AKR thymus-like alphabeta T cells. gammadelta T cells in athymic nu/nu mice with a fetal thymic graft predominantly expressed the donor Thy-1.1 antigen but not the host Thy-1.2 antigen. The induction of these T cells, however, could not be restored by coadministration of the B subunit with peritoneal cells from normal mice. These results suggest that the B subunit activates intraperitoneal gammadelta and alphabeta T cells in a manner dependent upon its ability to bind to GM1 ganglioside. gammadelta T cells induced by the B subunit are Th2-type cells derived from the thymus. These gammadelta T cells may be functionally involved in specific Th2 responses to the B subunit, which possibly acts as an adjuvant through the influence of alphabeta T cells.

Published
2005
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