Search

Your search keyword '"Shimada, Hiroyuki"' showing total 3,788 results
Start Over Author "Shimada, Hiroyuki"
3,788 results on '"Shimada, Hiroyuki"'

1. Label-free mid-infrared photothermal microscopy revisits intracellular thermal dynamics: what do fluorescent nanothermometers measure?

Author

Toda, Keiichiro, Takarada, Masaharu, Ishigane, Genki, Shimada, Hiroyuki, Badarla, Venkata Ramaiah, Okabe, Kohki, and Ideguchi, Takuro

Subjects
Physics - Biological Physics, Physics - Optics
Abstract

Fluorescent nanothermometry has revealed pronounced inhomogeneous temperature distributions within cells and has opened the field of single-cell thermal biology. However, this finding has sparked a controversial discussion known as the 10^5 gap issue, which arises from a simple heat conduction calculation suggesting such large temperature distributions should not exist inside cells. This debate has led to a hypothesis that intracellular thermal conduction is considerably slower than in water. Although various efforts have been made to measure intracellular thermal conduction, there are significant variations in the reported values. Here, we address this issue using label-free mid-infrared photothermal microscopy, which enables us to measure heat-induced temperature variations under local thermal equilibrium via refractive index changes. We measured intracellular thermal diffusivity via transient thermal decay and found that thermal diffusivity within living cells is 93-94% of water in the cytoplasm and nucleus. This result suggests that intracellular thermal conduction cannot explain the 10^5 gap issue. To investigate this issue further, we compared fluorescent nanothermometry with our label-free thermometry by measuring heat-induced temperature variations in living cells. This experiment revealed intriguing results where the fluorescent nanothermometry showed a slowly varying unexpected signal in addition to a constant temperature change. This result indicates that fluorescent nanothermometers are sensitive to additional factors on top of the temperature variation under local thermal equilibrium, raising an open question of what fluorescent nanothermometry measures.

Published
2024

6. Mid-infrared wide-field nanoscopy

Author

Tamamitsu, Miu, Toda, Keiichiro, Badarla, Venkata Ramaiah, Shimada, Hiroyuki, Konishi, Kuniaki, and Ideguchi, Takuro

Subjects
Physics - Optics
Abstract

Mid-infrared (MIR) spectroscopy is widely recognized as a powerful, non-distractive method for chemical analysis. However, its utility is constrained by a micrometer-scale spatial resolution imposed by the long-wavelength MIR diffraction limit. This limitation has been recently overcome by MIR photothermal (MIP) imaging, which detects photothermal effects induced in the vicinity of MIR absorbers using a visible-light microscope. Despite its promise, the full potential of its spatial resolving power has not been realized. Here, we present an optimal implementation of wide-field MIP imaging to achieve high spatial resolution. This is accomplished by employing single-objective synthetic-aperture quantitative phase imaging (SOSA-QPI) with synchronized sub-nanosecond MIR and visible light sources, effectively suppressing the resolution-degradation effect caused by photothermal heat diffusion. We demonstrate far-field MIR spectroscopic imaging with a spatial resolution limited by the visible diffraction, down to 125 nm, in the MIR region of 3.12-3.85 um (2,600-3,200 cm-1). This technique, through the use of a shorter visible wavelength and/or a higher objective numerical aperture, holds the potential to achieve a spatial resolution of less than 100 nm, thus paving the way for MIR wide-field nanoscopy.

Published
2023

10. Upconversion time-stretch infrared spectroscopy

Author

Hashimoto, Kazuki, Nakamura, Takuma, Kageyama, Takahiro, Badarla, Venkata Ramaiah, Shimada, Hiroyuki, Horisaki, Ryoich, and Ideguchi, Takuro

Subjects
Physics - Optics
Abstract

High-speed measurement confronts the extreme speed limit when the signal becomes comparable to the noise level. In the context of broadband mid-infrared spectroscopy, state-of-the-art ultrafast Fourier-transform infrared spectrometers, in particular dual-comb spectrometers, have improved the measurement rate up to a few Mspectra/s, which is limited by the signal-to-noise ratio. Time-stretch infrared spectroscopy, an emerging ultrafast frequency-swept mid-infrared spectroscopy technique, has shown a record-high rate of 80 Mspectra/s with an intrinsically higher signal-to-noise ratio than Fourier-transform spectroscopy by more than the square-root of the number of spectral elements. However, it can measure no more than ~30 spectral elements with a low resolution of several cm-1. Here, we significantly increase the measurable number of spectral elements to more than 1,000 by incorporating a nonlinear upconversion process. The one-to-one mapping of a broadband spectrum from the mid-infrared to the near-infrared telecommunication region enables low-loss time-stretching with a single-mode optical fiber and low-noise signal detection with a high-bandwidth photoreceiver. We demonstrate high-resolution mid-infrared spectroscopy of gas-phase methane molecules with a high resolution of 0.017 cm-1. This unprecedentedly high-speed vibrational spectroscopy technique would satisfy various unmet needs in experimental molecular science, e.g., measuring ultrafast dynamics of irreversible phenomena, statistically analyzing a large amount of heterogeneous spectral data, or taking broadband hyperspectral images at a high frame rate.

Published
2022

11. Mid-infrared photothermal single-live-cell imaging beyond video rate

Author

Ishigane, Genki, Toda, Keiichiro, Tamamitsu, Miu, Shimada, Hiroyuki, Badarla, Venkata Ramaiah, and Ideguchi, Takuro

Subjects
Physics - Optics
Abstract

Advancement in mid-infrared (MIR) technology has led to promising biomedical applications of MIR spectroscopy, such as liquid biopsy or breath diagnosis. On the contrary, MIR microscopy has been rarely used for live biological samples in an aqueous environment due to the lack of spatial resolution and the large water absorption background. Recently, mid-infrared photothermal (MIP) imaging has proven to be applicable to 2D and 3D single-cell imaging with high spatial resolution inherited from visible light. However, the maximum measurement rate has been limited to several frames/s, limiting its range of use. Here, we develop a significantly-improved wide-field MIP quantitative phase microscope with two orders-of-magnitude higher signal-to-noise ratio than previous MIP imaging techniques and demonstrate single-live-cell imaging beyond video rate. We first derive optimal system design by numerically simulating thermal conduction following the photothermal effect. Then, we develop the designed system with a homemade nanosecond MIR optical parametric oscillator and a high full-well-capacity image sensor. Our high-speed and high-spatial-resolution MIR microscope has great potential to become a new tool for life science, in particular for single-live-cell analysis.

Published
2022

13. Outcomes Following GD2-Directed Postconsolidation Therapy for Neuroblastoma After Cessation of Random Assignment on ANBL0032: A Report From the Childrens Oncology Group.

Author

Desai, Ami, Gilman, Andrew, Ozkaynak, Mehmet, Naranjo, Arlene, London, Wendy, Tenney, Sheena, Diccianni, Mitchell, Hank, Jacquelyn, Parisi, Marguerite, Shulkin, Barry, Smith, Malcolm, Moscow, Jeffrey, Shimada, Hiroyuki, Matthay, Katherine, Cohn, Susan, Maris, John, Bagatell, Rochelle, Sondel, Paul, Park, Julie, and Yu, Alice

Subjects
Child, Humans, Infant, Granulocyte-Macrophage Colony-Stimulating Factor, Interleukin-2, Research Design
Abstract

PURPOSE: Postconsolidation immunotherapy including dinutuximab, granulocyte-macrophage colony-stimulating factor, and interleukin-2 improved outcomes for patients with high-risk neuroblastoma enrolled on the randomized portion of Childrens Oncology Group study ANBL0032. After random assignment ended, all patients were assigned to immunotherapy. Survival and toxicities were assessed. PATIENTS AND METHODS: Patients with a pre-autologous stem cell transplant (ASCT) response (excluding bone marrow) of partial response or better were eligible. Demographics, stage, tumor biology, pre-ASCT response, and adverse events were summarized using descriptive statistics. Event-free survival (EFS) and overall survival (OS) from time of enrollment (up to day +200 from last ASCT) were evaluated. RESULTS: From 2009 to 2015, 1,183 patients were treated. Five-year EFS and OS for the entire cohort were 61.1 ± 1.9% and 71.9 ± 1.7%, respectively. For patients ≥ 18 months old at diagnosis with International Neuroblastoma Staging System stage 4 disease (n = 662) 5-year EFS and OS were 57.0 ± 2.4% and 70.9 ± 2.2%, respectively. EFS was superior for patients with complete response/very good partial response pre-ASCT compared with those with PR (5-year EFS: 64.2 ± 2.2% v 55.4 ± 3.2%, P = .0133); however, OS was not significantly different. Allergic reactions, capillary leak, fever, and hypotension were more frequent during interleukin-2-containing cycles than granulocyte-macrophage colony-stimulating factor-containing cycles (P < .0001). EFS was superior in patients with higher peak dinutuximab levels during cycle 1 (P = .034) and those with a high affinity FCGR3A genotype (P = .0418). Human antichimeric antibody status did not correlate with survival. CONCLUSION: Analysis of a cohort assigned to immunotherapy after cessation of random assignment on ANBL0032 confirmed previously described survival and toxicity outcomes. EFS was highest among patients with end-induction complete response/very good partial response. Among patients with available data, higher dinutuximab levels and FCGR3A genotype were associated with superior EFS. These may be predictive biomarkers for dinutuximab therapy.

Published
2022

21. Positron emission tomography and magnetic resonance imaging methods and datasets within the Dominantly Inherited Alzheimer Network (DIAN)

Author

McKay, Nicole S., Gordon, Brian A., Hornbeck, Russ C., Dincer, Aylin, Flores, Shaney, Keefe, Sarah J., Joseph-Mathurin, Nelly, Jack, Clifford R., Koeppe, Robert, Millar, Peter R., Ances, Beau M., Chen, Charles D., Daniels, Alisha, Hobbs, Diana A., Jackson, Kelley, Koudelis, Deborah, Massoumzadeh, Parinaz, McCullough, Austin, Nickels, Michael L., Rahmani, Farzaneh, Swisher, Laura, Wang, Qing, Allegri, Ricardo F., Berman, Sarah B., Brickman, Adam M., Brooks, William S., Cash, David M., Chhatwal, Jasmeer P., Day, Gregory S., Farlow, Martin R., la Fougère, Christian, Fox, Nick C., Fulham, Michael, Ghetti, Bernardino, Graff-Radford, Neill, Ikeuchi, Takeshi, Klunk, William, Lee, Jae-Hong, Levin, Johannes, Martins, Ralph, Masters, Colin L., McConathy, Jonathan, Mori, Hiroshi, Noble, James M., Reischl, Gerald, Rowe, Christopher, Salloway, Stephen, Sanchez-Valle, Raquel, Schofield, Peter R., Shimada, Hiroyuki, Shoji, Mikio, Su, Yi, Suzuki, Kazushi, Vöglein, Jonathan, Yakushev, Igor, Cruchaga, Carlos, Hassenstab, Jason, Karch, Celeste, McDade, Eric, Perrin, Richard J., Xiong, Chengjie, Morris, John C., Bateman, Randall J., and Benzinger, Tammie L. S.

Published
2023
Full Text
View/download PDF

43. Prevalence of family history of cancer in the NC-CCAPH consortium of Japan

Author

Abe, Sarah Krull, Ihira, Hikaru, Minami, Tetsuji, Imatoh, Takuya, Inoue, Yosuke, Tsutsumimoto, Kota, Kobayashi, Nozomu, Kashima, Rena, Konishi, Maki, Doi, Takehiko, Teramoto, Masayuki, Kabe, Isamu, Lee, Sangyoon, Watanabe, Makoto, Dohi, Seitaro, Sakai, Yukie, Nishita, Yukiko, Morisaki, Naho, Tachimori, Hisateru, Kokubo, Yoshihiro, Yamaji, Taiki, Shimada, Hiroyuki, Mizoue, Tetsuya, Sawada, Norie, Tsugane, Shoichiro, Iwasaki, Motoki, and Inoue, Manami

Published
2023
Full Text
View/download PDF

44. Lorlatinib with or without chemotherapy in ALK-driven refractory/relapsed neuroblastoma: phase 1 trial results

Author

Goldsmith, Kelly C., Park, Julie R., Kayser, Kimberly, Malvar, Jemily, Chi, Yueh-Yun, Groshen, Susan G., Villablanca, Judith G., Krytska, Kateryna, Lai, Lillian M., Acharya, Patricia T., Goodarzian, Fariba, Pawel, Bruce, Shimada, Hiroyuki, Ghazarian, Susan, States, Lisa, Marshall, Lynley, Chesler, Louis, Granger, Meaghan, Desai, Ami V., Mody, Rajen, Morgenstern, Daniel A., Shusterman, Suzanne, Macy, Margaret E., Pinto, Navin, Schleiermacher, Gudrun, Vo, Kieuhoa, Thurm, Holger C., Chen, Joseph, Liyanage, Marlon, Peltz, Gerson, Matthay, Katherine K., Berko, Esther R., Maris, John M., Marachelian, Araz, and Mossé, Yael P.

Published
2023
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results