40 results on '"Shikui Zhang"'
Search Results
2. Metabolomic and transcriptomice analyses of flavonoid biosynthesis in apricot fruits
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Yilin Chen, Wenwen Li, Kai Jia, Kang Liao, Liqiang Liu, Guoquan Fan, Shikui Zhang, and Yatong Wang
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apricot ,metabolomics ,transcriptomics ,flavonoid biosynthesis ,weighted gene coexpression network analysis ,Plant culture ,SB1-1110 - Abstract
IntroductionFlavonoids, as secondary metabolites in plants, play important roles in many biological processes and responses to environmental factors.MethodsApricot fruits are rich in flavonoid compounds, and in this study, we performed a combined metabolomic and transcriptomic analysis of orange flesh (JN) and white flesh (ZS) apricot fruits.Results and discussionA total of 222 differentially accumulated flavonoids (DAFs) and 15855 differentially expressed genes (DEGs) involved in flavonoid biosynthesis were identified. The biosynthesis of flavonoids in apricot fruit may be regulated by 17 enzyme-encoding genes, namely PAL (2), 4CL (9), C4H (1), HCT (15), C3’H (4), CHS (2), CHI (3), F3H (1), F3’H (CYP75B1) (2), F3’5’H (4), DFR (4), LAR (1), FLS (3), ANS (9), ANR (2), UGT79B1 (6) and CYP81E (2). A structural gene-transcription factor (TF) correlation analysis yielded 3 TFs (2 bHLH, 1 MYB) highly correlated with 2 structural genes. In addition, we obtained 26 candidate genes involved in the biosynthesis of 8 differentially accumulated flavonoids metabolites in ZS by weighted gene coexpression network analysis. The candidate genes and transcription factors identified in this study will provide a highly valuable molecular basis for the in-depth study of flavonoid biosynthesis in apricot fruits.
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- 2023
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3. PaPYL9 is involved in the regulation of apricot fruit ripening through ABA signaling pathway
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Mengxiao Jia, Jing Feng, Lina Zhang, Shikui Zhang, and Wanpeng Xi
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Apricot ,PYLs ,Abscisic acid ,Ethylene ,Fruit ripening ,Plant culture ,SB1-1110 - Abstract
Abscisic acid (ABA) is a major regulator of non-climacteric fruit ripening; however, the role of ABA in the ripening of climacteric fruit is not clear. Here, as a typical climacteric fruit, apricots were used to investigate the role of ABA in fruit ripening. Based on weighted gene co-expression network analysis (WGCNA) of our previous transcriptome data, we treated ‘Danxing’ fruit with exogenous ABA and obtained ABA receptor genes, genes related to ABA biosynthesis and signal transduction, and analyzed the response of these candidate genes to exogenous ABA during fruit ripening. Subsequently, the full length of candidate PYLs genes were cloned, and their putative function were analyzed by phylogenetic analysis and protein structure domain analysis. And then the function of one candidate gene PaPYL9 was verified by using transgenic tomato. Furthermore, the response genes in transgenic tomato were screened by transcriptome sequencing, and ultimately the related regulatory network was proposed. The results showed that the injection of exogenous 1.89 mmol·L-1 ABA remarkably promoted fruit coloration, and increased the color index for red grapes (CIRG) and the total soluble solids (TSS) content, but significantly decreased the firmness and titratable acid (TA) content (p
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- 2022
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4. Transcriptome analysis insight into ethylene metabolism and pectinase activity of apricot (Prunus armeniaca L.) development and ripening
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Min Xu, Weiquan Zhou, Wenjuan Geng, Shirong Zhao, Yan Pan, Guoquan Fan, Shikui Zhang, Yatong Wang, and Kang Liao
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Medicine ,Science - Abstract
Abstract Ethylene metabolism is very important for climacteric fruit, and apricots are typical climacteric fruit. The activity of pectinase is closely related to fruit firmness, which further affects fruit quality. To better understand ethylene metabolism, pectinase activity and their molecular regulation mechanisms during the development and ripening of apricot fruit, ethylene metabolism, pectinase activity and the “Luntaibaixing” apricot fruit transcriptome were analyzed at different developmental stages. Ethylene metabolic precursors, enzyme activities and ethylene release increased during fruit development and ripening, with significant differences between the ripening stage and other stages (P
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- 2021
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5. Karyotypic Characteristics and Genetic Relationships of Apricot Accessions from Different Ecological Groups
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Wenwen Li, Liqiang Liu, Weiquan Zhou, Yanan Wang, Xiang Ding, Guoquan Fan, Shikui Zhang, and Kang Liao
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chromosome number ,diversity ,karyotype analysis ,p. armeniaca ,Plant culture ,SB1-1110 - Abstract
The present study aims to reveal the karyotypic characteristics and genetic relationships of apricot (Prunus armeniaca L.) accessions from different ecological groups. Fourteen, 9, and 30 accessions from the Central Asian ecological group, North China ecological group, and Dzhungar-Ili ecological group, respectively, were analyzed according to the conventional pressing plate method. The results showed that all the apricot accessions from the different ecological groups were diploid (2n = 2x = 16). The total haploid length of the chromosome set of the selected accessions ranged from 8.11 to 12.75 μm, which was a small chromosome, and no satellite chromosomes were detected. All accessions had different numbers of median-centromere chromosomes or sub-median-centromere chromosomes. The karyotypes of the selected accessions were classified as 1A or 2A. Principal component analysis revealed that the long-arm/short-arm ratio (0.968) and the karyotype symmetry index (−0.979) were the most valuable parameters, and cluster analysis revealed that the accessions from the Central Asian ecological group and Dzhungar-Ili ecological group clustered together. In terms of karyotypic characteristics, the accessions from the Dzhungar-Ili ecological group and Central Asian ecological group were closely related.
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- 2020
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6. Identification of key genes and regulators associated with carotenoid metabolism in apricot (Prunus armeniaca) fruit using weighted gene coexpression network analysis
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Lina Zhang, Qiuyun Zhang, Wenhui Li, Shikui Zhang, and Wanpeng Xi
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Carotenoids ,Apricot ,Color ,WGCNA ,Prunus armeniaca L ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Carotenoids are a class of terpenoid pigments that contribute to the color and nutritional value of many fruits. Their biosynthetic pathways have been well established in a number of plant species; however, many details of the regulatory mechanism controlling carotenoid metabolism remain to be elucidated. Apricot is one of the most carotenoid-rich fruits, making it a valuable system for investigating carotenoid metabolism. The purpose of this study was to identify key genes and regulators associated with carotenoid metabolism in apricot fruit based on transcriptome sequencing. Results During fruit ripening in the apricot cultivar ‘Luntaixiaobaixing’ (LT), the total carotenoid content of the fruit decreased significantly, as did the levels of the carotenoids β-carotene, lutein and violaxanthin (p
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- 2019
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7. The R2R3-MYB transcription factor PaMYB10 is involved in anthocyanin biosynthesis in apricots and determines red blushed skin
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Wanpeng Xi, Jing Feng, Yu Liu, Shikui Zhang, and Guohua Zhao
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Prunus armeniaca ,Anthocyanin ,MYB transcription factor ,WGCNA ,Cloning ,qRT-PCR ,Botany ,QK1-989 - Abstract
Abstract Background The majority of apricot (Prunus armeniaca L.) cultivars display orange or yellow background skin, whereas some cultivars are particularly preferred by consumers because of their red blushed skin on the background. Results In this study, two blushed (‘Jianali’ and ‘Hongyu’) and two nonblushed (‘Baixing’ and ‘Luntaixiaobaixing’) cultivars were used to investigate the formation mechanism of blushed skin in apricots. High-performance liquid chromatography (HPLC) analysis showed that the blushed cultivars accumulated higher cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside and peonidin-3-O-rutinoside levels during fruit ripening than the nonblushed cultivars. Based on coexpression network analysis (WGCNA), a putative anthocyanin-related R2R3-MYB, PaMYB10, and seven structural genes were identified from transcriptome data. The phylogenetic analysis indicated that PaMYB10 clustered in the anthocyanin-related MYB clade. Sequence alignments revealed that PaMYB10 contained a bHLH-interaction motif ([DE]Lx2[RK]x3Lx6Lx3R) and an ANDV motif. Subcellular localization analysis showed that PaMYB10 was a nuclear protein. Real-time qRT-PCR analysis demonstrated that the transcript levels of PaMYB10 and seven genes responsible for anthocyanin synthesis were significantly higher in blushed than in nonblushed apricots, which was consistent with the accumulation of anthocyanin. In addition, bagging significantly inhibited the transcript levels of PaMYB10 and the structural genes in ‘Jianali’ and blocked the red coloration and anthocyanin accumulation. Transient PaMYB10 overexpression in ‘Luntaixiaobaixing’ fruits resulted in the red blushed skin at the maturation stage. Conclusions Taken together, these data reveal that three anthocyanins are responsible for the blushed skin of apricots, identify PaMYB10 as a positive regulator of anthocyanin biosynthesis in apricots, and demonstrate that blush formation depends on light.
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- 2019
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8. Cooperatively Improving Data Center Energy Efficiency Based on Multi-Agent Deep Reinforcement Learning
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Ce Chi, Kaixuan Ji, Penglei Song, Avinab Marahatta, Shikui Zhang, Fa Zhang, Dehui Qiu, and Zhiyong Liu
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data center ,energy efficiency ,deep reinforcement learning ,multi-agent ,scheduling algorithm ,cooling system ,Technology - Abstract
The problem of high power consumption in data centers is becoming more and more prominent. In order to improve the energy efficiency of data centers, cooperatively optimizing the energy of IT systems and cooling systems has become an effective way. In this paper, a model-free deep reinforcement learning (DRL)-based joint optimization method MAD3C is developed to overcome the high-dimensional state and action space problems of the data center energy optimization. A hybrid AC-DDPG cooperative multi-agent framework is devised for the improvement of the cooperation between the IT and cooling systems for further energy efficiency improvement. In the framework, a scheduling baseline comparison method is presented to enhance the stability of the framework. Meanwhile, an adaptive score is designed for the architecture in consideration of multi-dimensional resources and resource utilization improvement. Experiments show that our proposed approach can effectively reduce energy for data centers through the cooperative optimization while guaranteeing training stability and improving resource utilization.
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- 2021
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9. A new meta-heuristic task scheduling algorithm for optimizing energy efficiency in data centers.
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Shikui Zhang, Ce Chi, Kaixuan Ji, Zhiyong Liu 0002, Fa Zhang 0001, Penglei Song, Huimei Yuan, Dehui Qiu, and Xiaohua Wan
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- 2021
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10. A Deep Reinforcement Learning-based Task Scheduling Algorithm for Energy Efficiency in Data Centers.
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Penglei Song, Ce Chi, Kaixuan Ji, Zhiyong Liu 0002, Fa Zhang 0001, Shikui Zhang, Dehui Qiu, and Xiaohua Wan
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- 2021
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11. Pollen morphology of selected apricot (Prunus) taxa
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Shikui Zhang, Yanan Wang, Kang Liao, Yatong Wang, Yingying Niu, Shirong Zhao, Liqiang Liu, and Wenwen Li
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Palynology ,010506 paleontology ,Phylogenetic tree ,biology ,Prunus sibirica ,Paleontology ,010502 geochemistry & geophysics ,medicine.disease_cause ,biology.organism_classification ,01 natural sciences ,Prunus armeniaca ,Prunus ,Taxon ,Pollen ,Botany ,medicine ,Domestication ,0105 earth and related environmental sciences - Abstract
The purposes of this study were to provide palynological information about apricots and to reveal the relationships among six ecological groups of apricots by describing the morphological characteristics of their pollen. The pollen grains of 175 accessions belonging to three species (Prunus armeniaca L., Prunus sibirica L., and Prunus mume Sieb. et Zucc.) of Prunus were examined using light microscopy and scanning electron microscopy. The pollen grains were isopolar monads, radially symmetric, medium to large in size, prolate or subprolate, and 3-colporate. Most pollen grains exhibited striate exine ornamentation, and a few exhibited striato-reticulate or cerebroid exine ornamentation. Cluster analysis divided the six ecological groups into two groups: one including the Central Asian, North China, and Dzhungar-Ili ecological group accessions and another including the Northeast Asian, European, and East China ecological group accessions. Principal component analysis revealed that equatorial diameter, colpus length, colpus width, and ridge width were the most important metrical pollen traits, and can be used as powerful diagnostic characters in apricot identification. We speculated that the cultivated apricots in Xinjiang were domesticated from wild apricots from the Ili Valley. This study describes the morphological characteristics of apricot pollen grains and provides some morphological information for future studies on the phylogenetic relationships of apricots.
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- 2020
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12. Pathogenic Photobacterium sp. induce mortality in the lined seahorse (Hippocampus erectus): First case report from China
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Shouyuan ZHANG, Shikui ZHANG, Xiaohui BAI, Zhang LUO, Jiabing LIU, and Ke KE
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Aquatic Science ,Agronomy and Crop Science - Abstract
In August 2019, an unknown disease was first reported in farmed seahorses (Hippocampus erectus) in Binhai New Area in Tianjin city, China, with a cumulative mortality rate of 25% within seven days of onset. The main symptoms of the affected seahorses were discoloration of the body surface, abdominal distention, kidney erosion, and intestinal enlargement. Histopathological observation revealed apparent damage to the diseased seahorses’ intestines, liver, and kidneys. Strains HM-2019-5 and HM-2019-6 were isolated from diseased kidneys and identified as Photobacterium sp. based on physiological and biochemical tests and 16S rDNA sequencing analysis. Artificial infection experiments demonstrated that strain HM-2019-5 could cause a 90% morbidity rate in healthy seahorses. Antimicrobial susceptibility tests showed that HM-2019-5 and HM-2019-6 were resistant to 13 of 16 antimicrobial agents tested. This is the first report of photobacterial disease in a seahorse to the best of our knowledge.
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- 2022
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13. Identification of Key Genes Controlling Carotenoid Metabolism during Apricot Fruit Development by Integrating Metabolic Phenotypes and Gene Expression Profiles
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Yingying Niu, Shikui Zhang, Shaowen Quan, Shirong Zhao, Weiquan Zhou, Yatong Wang, Kang Liao, Min Xu, Mansur Nasier, and Guoquan Fan
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Prunus armeniaca ,medicine.medical_treatment ,macromolecular substances ,Transcriptome ,Gene Expression Regulation, Plant ,polycyclic compounds ,medicine ,Food science ,Gene ,Carotenoid ,chemistry.chemical_classification ,biology ,organic chemicals ,Flesh ,Carotene ,Structural gene ,food and beverages ,General Chemistry ,biology.organism_classification ,Carotenoids ,biological factors ,Metabolic pathway ,Phenotype ,chemistry ,Fruit ,General Agricultural and Biological Sciences - Abstract
To explore the metabolic basis of carotenoid accumulation in different developmental periods of apricot fruits, targeted metabonomic and transcriptomic analyses were conducted in four developmental periods (S1-S4) in two cultivars (Prunus armeniaca cv. "Kuchebaixing" with white flesh and P. armeniaca cv. "Shushangganxing" with orange flesh) with different carotenoid contents. 14 types of carotenes and 27 types of carotene lipids were identified in apricot flesh in different developmental periods. In S3 and S4, the carotenoid contents of the two cultivars were significantly different, and β-carotene and (E/Z)-phytoene were the key metabolites that caused the difference in the total carotenoid content between the examined cultivars. Twenty-five structural genes (including genes in the methylerythritol 4-phosphate and carotenoid biosynthesis pathways) related to carotenoid biosynthesis were identified among the differentially expressed genes in different developmental periods of the two cultivars, and a carotenoid metabolic pathway map of apricot fruits was drawn according to the KEGG pathway map. The combined analysis of carotenoid metabolism data and transcriptome data showed that PSY, NCED1, and CCD4 were the key genes leading to the great differences in the total carotenoid content. The results provide a new approach to study the synthesis and accumulation of carotenoids in apricot fruits.
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- 2021
14. Application of Robot Positioning for Cannulated Screw Internal Fixation in the Treatment of Femoral Neck Fracture: Retrospective Study
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Zhihao Li, Dalong Wu, Xiangyun Zhang, Dongtao Yuan, Lei Wan, Junming Li, Shao'an Zhang, Shikui Zhang, and Long Yue
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medicine.medical_specialty ,Percutaneous ,medicine.medical_treatment ,Computer applications to medicine. Medical informatics ,R858-859.7 ,Health Informatics ,Bone healing ,Femoral Neck Fractures ,03 medical and health sciences ,clinical efficacy ,0302 clinical medicine ,Health Information Management ,Medicine ,Internal fixation ,Femoral neck ,Fixation (histology) ,030222 orthopedics ,Hip fracture ,Original Paper ,femoral neck fracture ,business.industry ,robot positioning ,medicine.disease ,Surgery ,medicine.anatomical_structure ,percutaneous cannulated screw fixation ,prognosis ,business ,030217 neurology & neurosurgery ,Computer technology - Abstract
Background Femoral neck fracture is a common type of hip fracture. Conventional surgical treatment aims at fixing the fracture site with screws and then gradually promoting bone healing. A robot-assisted orthopedic surgery system is computer technology applied to surgical treatment. Objective This study aimed to explore the therapeutic effect and prognostic value of percutaneous cannulated screw internal fixation using robot-assisted positioning in patients with femoral neck fractures. Methods From July 2018 to September 2019, 42 cases of femoral neck fracture admitted to the Second Affiliated Hospital of Luohe Medical College were randomly and averagely divided into control and study groups. The patients in the control group were treated with conventional percutaneous cannulated screw internal fixation, while the patients in the study group were treated with robot-assisted percutaneous cannulated screw fixation during surgical treatment. We compared the treatment conditions and results of the operation between the 2 groups. The Harris score was used to evaluate the treatment efficacy. The state of fracture healing was followed up and compared between the 2 groups. Results The duration of the operation was shorter, there was less fluoroscopy use, and there were fewer drilled holes in the study group than in the control group (all, P Conclusions The use of robotic positioning aids in the treatment of femoral neck fractures with percutaneous cannulated screw fixation can effectively improve the efficiency of surgery, shorten the duration of surgery, and reduce the radiation damage to patients. Meanwhile, it improves postoperative treatment and recovery rates of the patients and shortens the fracture healing time.
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- 2021
15. Application of Robot Positioning for Cannulated Screw Internal Fixation in the Treatment of Femoral Neck Fracture: Retrospective Study (Preprint)
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Lei Wan, Xiangyun Zhang, Dalong Wu, Zhihao Li, Dongtao Yuan, Junming Li, Shikui Zhang, Long Yue, and Shao'an Zhang
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BACKGROUND Femoral neck fracture is a common type of hip fracture. Conventional surgical treatment aims at fixing the fracture site with screws and then gradually promoting bone healing. A robot-assisted orthopedic surgery system is computer technology applied to surgical treatment. OBJECTIVE This study aimed to explore the therapeutic effect and prognostic value of percutaneous cannulated screw internal fixation using robot-assisted positioning in patients with femoral neck fractures. METHODS From July 2018 to September 2019, 42 cases of femoral neck fracture admitted to the Second Affiliated Hospital of Luohe Medical College were randomly and averagely divided into control and study groups. The patients in the control group were treated with conventional percutaneous cannulated screw internal fixation, while the patients in the study group were treated with robot-assisted percutaneous cannulated screw fixation during surgical treatment. We compared the treatment conditions and results of the operation between the 2 groups. The Harris score was used to evaluate the treatment efficacy. The state of fracture healing was followed up and compared between the 2 groups. RESULTS The duration of the operation was shorter, there was less fluoroscopy use, and there were fewer drilled holes in the study group than in the control group (all, PP=.33). The Harris score (P=.045) and number of excellent and good ratings (P=.01) were significantly higher in the study group than in the control group. The difference in the fracture healing rate between the 2 groups was not statistically significant (P=.23). The fracture healing duration of the study group was shorter than that of the control group (P=.001). CONCLUSIONS The use of robotic positioning aids in the treatment of femoral neck fractures with percutaneous cannulated screw fixation can effectively improve the efficiency of surgery, shorten the duration of surgery, and reduce the radiation damage to patients. Meanwhile, it improves postoperative treatment and recovery rates of the patients and shortens the fracture healing time.
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- 2020
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16. Genetic diversity, population structure, and relationships of apricot (Prunus) based on restriction site-associated DNA sequencing
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Guoquan Fan, Qiu-Ping Zhang, Yatong Wang, Kang Liao, Liqiang Liu, Wenwen Li, Shikui Zhang, and Yanan Wang
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0106 biological sciences ,0301 basic medicine ,Germplasm ,Genetic diversity ,biology ,Prunus sibirica ,Population genetics ,Plant Science ,Horticulture ,biology.organism_classification ,01 natural sciences ,Biochemistry ,Prunus armeniaca ,Nucleotide diversity ,Gene flow ,03 medical and health sciences ,030104 developmental biology ,Evolutionary biology ,Genetic structure ,Genetics ,010606 plant biology & botany ,Biotechnology - Abstract
Single-nucleotide polymorphisms (SNPs) are the most abundant form of genomic polymorphisms and are widely used in population genetics research. Here, high-throughput sequencing was used to examine the genome-level diversity, population structure, and relationships of apricot, which are important for germplasm conservation and molecular breeding. Restriction site-associated DNA sequencing (RAD-seq) was adopted to sequence 168 Prunus spp. accessions distributed in five ecological groups, including 74 accessions of cultivated Prunus armeniaca L. and 94 accessions of wild apricots (P. armeniaca L. and Prunus sibirica L.), which generated 417,961 high-quality SNPs. We used cluster, genetic structure, and principal component analyses to examine the genetic diversities and genetic relationships of the 168 accessions. The Dzhungar-Ili ecological group accessions showed the highest genetic diversity in terms of private allele number, observed heterozygosity, and nucleotide diversity. We speculate that the Central Asian ecological group accessions were domesticated from the Dzhungar-Ili ecological group accessions. The population structure and gene flow of the North China and European ecological group accessions suggested a genetic background of P. sibirica. We argue that the two groups should be considered hybrid swarms connected to P. sibirica by continuous and extensive gene flow. P. armeniaca originated in Northwest China (Ili Valley), subsequently spread throughout Central Asia, and eventually spread to Europe. In addition, selective sweep signatures in P. armeniaca during domestication from wild to cultivated apricots, combined with differentially expressed genes, underlie distinct fruit traits, including sugars, aromas, organic acids, and carotenoids. This study provides substantive and valuable genomic resources that will significantly advance apricot improvement and effective utilization.
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- 2020
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17. Targeting PRMT5/Akt signalling axis prevents human lung cancer cell growth
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Yaqiong Ma, Xiaoyan Hu, Shikui Zhang, Yonghua Zheng, and Xiaoke Chen
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0301 basic medicine ,PTEN ,Protein-Arginine N-Methyltransferases ,Lung Neoplasms ,proliferation ,Adenocarcinoma of Lung ,Apoptosis ,03 medical and health sciences ,0302 clinical medicine ,Cyclin D1 ,Biomarkers, Tumor ,Tumor Cells, Cultured ,medicine ,Humans ,RNA, Small Interfering ,Lung cancer ,Protein kinase B ,PI3K/AKT/mTOR pathway ,Cell Proliferation ,biology ,Cell growth ,Chemistry ,Akt ,TOR Serine-Threonine Kinases ,PTEN Phosphohydrolase ,Original Articles ,Cell Biology ,Prognosis ,medicine.disease ,Gene Expression Regulation, Neoplastic ,lung cancer ,Cyclin E1 ,Cell Transformation, Neoplastic ,030104 developmental biology ,030220 oncology & carcinogenesis ,mTOR ,biology.protein ,Cancer research ,PRMT5 ,Molecular Medicine ,Phosphorylation ,Original Article ,Proto-Oncogene Proteins c-akt ,Signal Transduction - Abstract
The emerging evidence reveals that protein arginine methyltransferase 5 (PRMT5) is involved in regulation of tumour cell proliferation and cancer development. Nevertheless, the exact role of PRMT5 in human lung cancer cell proliferation and the underlying molecular mechanism remains largely obscure. Here, we showed that PRMT5 was highly expressed in human lung cancer cells and lung cancer tissues. Furthermore, we generated PRMT5 stable knockdown cell lines (A549 and H1299 cells) and explored the functions of PRMT5 in lung cancer cell proliferation. We found that the down‐regulation of PRMT5 by shRNA or the inhibition of PRMT5 by specific inhibitor GSK591 dramatically suppressed cyclin E1 and cyclin D1 expression and cell proliferation. Moreover, we uncovered that PRMT5 promoted lung cancer cell proliferation via regulation of Akt activation. PRMT5 was directly co‐localized and interacted with Akt, but not PTEN and mTOR. Down‐regulation or inhibition of PRMT5 markedly reduced Akt phosphorylation at Thr308 and Ser473, whereas the expression of PTEN and mTOR phosphorylation was unchanged, indicating that PRMT5 was an important upstream regulator of Akt and induced lung cancer cell proliferation. Altogether, our results indicate that PRMT5 promotes human lung cancer cell proliferation through direct interaction with Akt and regulation of Akt activity. Our findings also suggest that targeting PRMT5 may have therapeutic potential for treatment of human lung cancer.
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- 2018
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18. Inhalation lung injury induced by smoke bombs in children: CT manifestations, dynamic evolution features and quantitative analysis
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Xiaorui Ru, Gang Huang, Zeqing Mao, Xing Zhou, Lianping Zhao, Shikui Zhang, Ya-Qiong Ma, and Huaxin Xu
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030203 arthritis & rheumatology ,Pulmonary and Respiratory Medicine ,Bronchus ,medicine.medical_specialty ,Inhalation ,business.industry ,Smoke bomb ,Lung injury ,medicine.disease ,Pulmonary function testing ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,030228 respiratory system ,medicine ,Original Article ,Radiology ,Pneumomediastinum ,medicine.symptom ,Stage (cooking) ,business ,Subcutaneous emphysema - Abstract
Background: This retrospective study aimed to investigate the computed tomography (CT) manifestations, short-term dynamic evolution features and quantitative lung CT analysis of inhalation lung injury induced by smoke bomb flare. Methods: Eleven pediatric patients (aged 11 to 13) who inhaled the smoke of smoke bombs underwent several low-dose chest CT scans. The image characteristics and their dynamic changes were observed and quantitative CT values were analyzed. The quantitative CT indicators included lung injury CT score (LICTS), lung fibrosis CT score (LFCTS), mean lung density (MLD), normally aerated volume ratio (NAVR) and reductively aerated volume ratio (RAVR). Box-plot was used to analyze the dynamic changes of each indicator and Spearman statistical method was used to analyze the correlation between any two indicators. Results: (I) In most cases, there were multiple consolidation and massive ground-glass opacities (GGOs) in the two lungs, which aggravated in the early stage and then gradually dissoluted in the later stage. LICTS was positively correlated with MLD (r=0.811, P=0.000), while it was negatively correlated with NAVR (r=−0.712, P=0.000). There existed interstitial fibrosis in the later stage, and LFCTS was positively correlated with RAVR (r=0.382, P=0.028). (II) In one case, the patterns were like layered cake, i.e., consolidation with air bronchus signs in the accumulation area, GGOs in the aforementioned area and normal lung in the top area. The patterns aggravated in the early stage and quickly dissolved in the later stage, and only a few residual fibrotic lesions existed on the final scan. (III) For severe cases, pneumomediastinum and subcutaneous emphysema aggravated in the early stage and then gradually dissolved in the later stage. Conclusions: The chest CT manifestations of inhalation lung injury induced by smoke bombs are predominantly GGOs and consolidation. They aggravate in the early stage and gradual dissolute in the later stage. CT quantitative values can contribute to evaluating the extent of this disease, and NAVR and RAVR can be used to assess pulmonary function.
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- 2018
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19. Vitamin B6 as a novel risk biomarker of fractured ankles
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Zhihao Li, Dongtao Yuan, Xiaoyan Song, Dalong Wu, Shikui Zhang, Lei Wan, Shao'an Zhang, and Jiankui Jiang
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Adult ,Male ,medicine.medical_specialty ,Multivariate statistics ,Osteoporosis ,Observational Study ,Ankle Fractures ,Logistic regression ,vitamin B6 ,Risk Factors ,Internal medicine ,estrogen ,medicine ,Humans ,Prospective Studies ,Prospective cohort study ,Aged ,business.industry ,Incidence ,Incidence (epidemiology) ,Hazard ratio ,General Medicine ,Middle Aged ,medicine.disease ,osteoporosis ,Vitamin B 6 ,Confidence interval ,Causality ,medicine.anatomical_structure ,Female ,fractured ankles ,prognosis ,Ankle ,business ,Biomarkers ,Research Article - Abstract
Ankle fractures are the most common intra-articular fractures. Osteoporosis is a common and frequent disease among the elderly with a poor prognosis and high risk of fractured ankles. However, the relationship between vitamin B6 and the incidence of fractured ankles in patients with osteoporosis is unclear. A total of 101 patients with osteoporosis were recruited. Clinical and followed-up information was recorded. And the vitamin B6, albumin, globulin, and hemoglobin in the blood were tested. Pearson's chi-squared and spearman test were performed to analyze the correlation between fractured ankles and relative parameters. Univariate and multivariate logistic regression, receiver operating characteristic curve analysis, univariate and multivariate Cox proportional hazards regression analysis, and Kaplan–Meier method were also performed. There exist strong relation between the expression level of vitamin B6 and fractured ankle (P
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- 2021
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20. Analysis of carotenoid content and diversity in apricots (Prunus armeniaca L.) grown in China
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Shirong Zhao, Kang Liao, Yingying Niu, Shikui Zhang, Weiquan Zhou, Yalan Li, Guoquan Fan, and Xiang Ding
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Lutein ,China ,Prunus armeniaca ,01 natural sciences ,Analytical Chemistry ,Metabolic diversity ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Tandem Mass Spectrometry ,Cultivar ,Carotenoid ,Chromatography, High Pressure Liquid ,chemistry.chemical_classification ,biology ,Flesh ,010401 analytical chemistry ,04 agricultural and veterinary sciences ,General Medicine ,biology.organism_classification ,040401 food science ,Carotenoids ,0104 chemical sciences ,Horticulture ,Apocarotenal ,chemistry ,Fruit ,Food Science - Abstract
The carotenoids in the peel and flesh of 41 apricot cultivars were qualitatively and quantitatively analysed by UHPLC-APCI-MS/MS, and the L*, a*, b* and quality indexes of the fruits were determined. The results showed that the L*, a*, b* and quality indexes of the fruits were quite different, and 13 carotenoids were detected in the peel and flesh of apricots, among which e-carotene, α-cryptoxanthin and apocarotenal were newly detected carotenoids in apricots. The total carotenoid content of the 41 apricot cultivars varied from 20.983 to 320.278 μg/g FW, and the total carotenoid content varied from 17.353 to 222.098 μg/g FW in the peel and from 2.536 to 98.179 μg/g FW in the flesh. The main components of apricot fruits were β-carotene and (E/Z)-phytoene, followed by β-cryptoxanthin and lutein. This study shows that carotenoids in apricot fruits have rich metabolic diversity.
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- 2020
21. Genetic diversity, population structure, and relationships of apricot (
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Wenwen, Li, Liqiang, Liu, Yanan, Wang, Qiuping, Zhang, Guoquan, Fan, Shikui, Zhang, Yatong, Wang, and Kang, Liao
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Comparative genomics ,Article ,Genome evolution - Abstract
Single-nucleotide polymorphisms (SNPs) are the most abundant form of genomic polymorphisms and are widely used in population genetics research. Here, high-throughput sequencing was used to examine the genome-level diversity, population structure, and relationships of apricot, which are important for germplasm conservation and molecular breeding. Restriction site-associated DNA sequencing (RAD-seq) was adopted to sequence 168 Prunus spp. accessions distributed in five ecological groups, including 74 accessions of cultivated Prunus armeniaca L. and 94 accessions of wild apricots (P. armeniaca L. and Prunus sibirica L.), which generated 417,961 high-quality SNPs. We used cluster, genetic structure, and principal component analyses to examine the genetic diversities and genetic relationships of the 168 accessions. The Dzhungar-Ili ecological group accessions showed the highest genetic diversity in terms of private allele number, observed heterozygosity, and nucleotide diversity. We speculate that the Central Asian ecological group accessions were domesticated from the Dzhungar-Ili ecological group accessions. The population structure and gene flow of the North China and European ecological group accessions suggested a genetic background of P. sibirica. We argue that the two groups should be considered hybrid swarms connected to P. sibirica by continuous and extensive gene flow. P. armeniaca originated in Northwest China (Ili Valley), subsequently spread throughout Central Asia, and eventually spread to Europe. In addition, selective sweep signatures in P. armeniaca during domestication from wild to cultivated apricots, combined with differentially expressed genes, underlie distinct fruit traits, including sugars, aromas, organic acids, and carotenoids. This study provides substantive and valuable genomic resources that will significantly advance apricot improvement and effective utilization.
- Published
- 2019
22. Pathogenic Photobacterium sp. induces mortality in the lined seahorse (Hippocampus erectus): China's first case report.
- Author
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Shouyuan ZHANG, Shikui ZHANG, Xiaohui BAI, Zhang LUO, Jiabing LIU, and Ke KE
- Subjects
PHOTOBACTERIUM ,MORTALITY ,SEA horses ,HISTOPATHOLOGY ,ANTIOXIDANTS - Abstract
In August 2019, an unknown disease was first reported in farmed seahorses (Hippocampus erectus) in Binhai New Area in Tianjin city, China, with a cumulative mortality rate of 25% within seven days of onset. The main symptoms of the affected seahorses were discoloration of the body surface, abdominal distention, kidney erosion, and intestinal enlargement. Histopathological observation revealed damage to the diseased seahorses' intestines, liver, and kidneys. Strains HM-2019-5 and HM-2019-6 were isolated from diseased kidneys and identified as Photobacterium sp. based on physiological and biochemical tests and 16S rDNA sequencing analysis. Artificial infection experiments demonstrated that strain HM-2019-5 could cause a 90% morbidity rate in healthy seahorses. Antimicrobial susceptibility tests showed that HM-2019-5 and HM-2019-6 were resistant to 13 of 16 antimicrobial agents tested. This is the first report of photobacterial disease in a seahorse to the best of our knowledge. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
23. Additional file 6: of The R2R3-MYB transcription factor PaMYB10 is involved in anthocyanin biosynthesis in apricots and determines red blushed skin
- Author
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Wanpeng Xi, Feng, Jing, Liu, Yu, Shikui Zhang, and Guohua Zhao
- Abstract
Table S5. The primer sequences for 5â ˛ RACE and 3â ˛ RACE. (PDF 351 kb)
- Published
- 2019
- Full Text
- View/download PDF
24. MOESM3 of Identification of key genes and regulators associated with carotenoid metabolism in apricot (Prunus armeniaca) fruit using weighted gene coexpression network analysis
- Author
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Zhang, Lina, Qiuyun Zhang, Wenhui Li, Shikui Zhang, and Wanpeng Xi
- Abstract
Additional file 3. Gene ontology (GO) classification map. The horizontal axis represents the type of GO function; the vertical axis on the right represents the number of unigenes annotated to the corresponding GO function; and the vertical axis on the left represents the percentage of the number of unigenes within the total.
- Published
- 2019
- Full Text
- View/download PDF
25. Additional file 4: of The R2R3-MYB transcription factor PaMYB10 is involved in anthocyanin biosynthesis in apricots and determines red blushed skin
- Author
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Wanpeng Xi, Feng, Jing, Liu, Yu, Shikui Zhang, and Guohua Zhao
- Abstract
Table S3. Flavonoid-related MYB transcription factors using in phylogenetic analysis. (PDF 227 kb)
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- 2019
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26. Additional file 3: of The R2R3-MYB transcription factor PaMYB10 is involved in anthocyanin biosynthesis in apricots and determines red blushed skin
- Author
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Wanpeng Xi, Feng, Jing, Liu, Yu, Shikui Zhang, and Guohua Zhao
- Subjects
animal structures ,fungi - Abstract
Figure S1. Phylogenetic tree of gene sequences for 148 MYB genes from the apricot transcriptome and MYB transcription factors from other species. (PDF 823 kb)
- Published
- 2019
- Full Text
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27. Additional file 5: of The R2R3-MYB transcription factor PaMYB10 is involved in anthocyanin biosynthesis in apricots and determines red blushed skin
- Author
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Wanpeng Xi, Feng, Jing, Liu, Yu, Shikui Zhang, and Guohua Zhao
- Abstract
Table S4. The primer sequences for qRT-PCR. (PDF 363 kb)
- Published
- 2019
- Full Text
- View/download PDF
28. Additional file 2: of The R2R3-MYB transcription factor PaMYB10 is involved in anthocyanin biosynthesis in apricots and determines red blushed skin
- Author
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Wanpeng Xi, Feng, Jing, Liu, Yu, Shikui Zhang, and Guohua Zhao
- Abstract
Table S2. Candidate genes involved in anthocyanin biosynthesis in apricot fruit. (PDF 214 kb)
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- 2019
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29. Additional file 1: of The R2R3-MYB transcription factor PaMYB10 is involved in anthocyanin biosynthesis in apricots and determines red blushed skin
- Author
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Wanpeng Xi, Feng, Jing, Liu, Yu, Shikui Zhang, and Guohua Zhao
- Abstract
Table S1. Color indexes of apricot fruits at different developmental stages. (PDF 251 kb)
- Published
- 2019
- Full Text
- View/download PDF
30. Genome size and chromosome ploidy identification in pear germplasm represented by Asian pears - Local pear varieties
- Author
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Xiangying Chen, Guoquan Fan, Yingying Niu, Shikui Zhang, Weiquan Zhou, and Kang Liao
- Subjects
0106 biological sciences ,0301 basic medicine ,Pyrus ussuriensis ,Germplasm ,PEAR ,biology ,Horticulture ,biology.organism_classification ,01 natural sciences ,03 medical and health sciences ,030104 developmental biology ,Polyploid ,Pyrus × sinkiangensis ,Ploidy ,Genome size ,010606 plant biology & botany ,Pyrus communis - Abstract
Pear (Pyrus) germplasm resources in Xinjiang were rich, most of which were diploid. Including dozens of varieties such as Pyrus bretschneideri Rehd. (P-br), Pyrus pyrifolia Nakai. (P-py), Pyrus ussuriensis Maxim. (P-us), Pyrus communis L. (P-co), Pyrus betulifolia Bunge. (P-be), Pyrus sinkiangensis Yu. (P-si). The P-si was mainly distributed in Shanshan, Turpan, Korla, Aksu and other places. The genetic background of the main varietieswas not completely clear.The genome size and chromosome ploidy of main pear varieties in Xinjiang were identified by flow cytometry (FCM) and the traditional chromosome pressing method. The results showed that, the genome size of P-xj was 566.54 ± 125.17 Mb, slightly larger than that of the introduced varieties, with a 2C values of 1.16 ± 0.26 pg and a ploidy value (DI) of 1.1 ± 0.24. Twenty-four P-xj were diploid with 34 chromosome numbers. The genome sizes of introduced P-br, P-py, P-us, P-be, and P-co were 485.44 ± 10.05∼567.94 ± 44.40 Mb, and the 2C values was 0.99 ± 0.02∼1.16 ± 0.10 pg. The DI was 0.9 ± 0.06∼1.0 ± 0.09, the FCM detection indicated that they were diploid; the number of chromosomes was 34.There were differences in genome size between pear with the same ploidy. The genome size of all diploid pear was 480.95 ± 16.24∼599.14 ± 38.36 Mb, the 2C values was 0.98 ± 0.03 pg ∼1.23 ± 0.08 pg, and the DI was 0.9 ± 0.00∼1.2 ± 0.06. A total of 5 polyploid materials were found in this study: ‘Yilikamut’, ‘Kotoamut’, ‘Aiwenqieke’, ‘Heisuanli’ and ‘Sha01′, it can provide materials for the innovation and utilization of pear germplasm resources. The results of this study can provide technical support for Pyrus plant on ploidy breeding and omics study, and also a reference for other plants.
- Published
- 2020
- Full Text
- View/download PDF
31. [Effects of curcumin on pulmonary fibrosis and functions of paraquat-challenged rats]
- Author
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Honggang, Chen, Rongjia, Yang, Yan, Tang, Jun, Xu, Youfan, Feng, Shijie, Liu, Shikui, Zhang, and Lijun, Hou
- Subjects
Paraquat ,Rats, Sprague-Dawley ,Transforming Growth Factor beta1 ,Curcumin ,Pulmonary Fibrosis ,Animals ,Rats, Wistar ,Lung ,Rats - Abstract
To observe the effects of curcumin on pulmonary fibrosis and functions on paraquat (PQ)-challenged rats, and investigate the possible mechanism.108 SPF Wistar rats were divided into three groups according to random number sheet: normal saline (NS) control group, PQ model group and curcumin-treatment group. The rats in each group were subdivided into three subgroups according to different time points (3, 7, 14 days), with 12 rats in each subgroup. PQ-challenged models were reproduced by intragastrical administration of PQ solution 50 mg/kg, and those in NS control group were given the equal volume of NS. After 30 minutes, the rats in curcumin-treatment group were given 200 mg/kg of curcumin by intraperitoneal injection, and those in NS control group and PQ model group were given the equal volume of NS. At 3, 7, 14 days, the tidal volume (VT) was examined, and the blood was drawn from femoral artery for blood gas analysis. Then the rats were sacrificed and the lung tissues were harvested, the hydroxyproline (Hyp) content was measured by alkaline hydrolysis; the expression of transforming growth factor-β1 (TGF-β1) was determined by immuno-histochemistry; the distribution and the change of the pulmonary collagen fiber were observed after Masson staining.After exposure to PQ, the VT and arterial partial pressure of oxygen (PaOCurcumin can enhance the pulmonary function by reducing the deposition of collagen fiber and inhabiting pulmonary fibrosis of PQ-poisoned rats.
- Published
- 2017
32. Determination of genome size and chromosome ploidy of selected taxa from Prunus armeniaca by flow cytometry
- Author
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Yatong Wang, Kang Liao, Shikui Zhang, Yanan Wang, Wenwen Li, Guoquan Fan, and Liqiang Liu
- Subjects
0106 biological sciences ,0301 basic medicine ,medicine.medical_specialty ,Chromosome ,Horticulture ,Biology ,biology.organism_classification ,01 natural sciences ,Prunus armeniaca ,Crop ,03 medical and health sciences ,030104 developmental biology ,Taxon ,Molecular genetics ,Botany ,medicine ,Ploidy ,Gene ,Genome size ,010606 plant biology & botany - Abstract
The assessment of plant genome size is important in many research fields, including crop improvement and molecular genetics. However, studies on the genome size of Prunus armeniaca is limited. The purpose of this study was to evaluate the differences in the genome size of P. armeniaca in different geographic ecological groups and estimate the ranges in genome size. Nuclear suspension of P. armeniaca was prepared by Marie's isolation buffer and propidium iodide fluorescent dye. The genome size of 51, 60, 11 and 2 accessions from the Central Asian Ecological Group, Dzhungar-ili Ecological Group, North China Ecological Group and European Ecological Group, respectively, were assessed by flow cytometry, and the levels of ploidy were evaluated. There were no polyploids from different ecological groups of P. armeniaca; all accessions were diploids. The genome size of P. armeniaca varied from 0.590 to 0.708 pg, and the average genome size was 0.637 pg. The European Ecological Group had the largest genome size (0.661 pg/2C), which was the only group significantly different in size from the Central Asian, North China and Dzhungar-ili Ecological Group (P = 0.05), while the difference in genome size among these three ecological groups was small. There was no significant difference in genome size between different populations of wild accessions, and there was no significant correlation with longitude, latitude and altitude. The variation in the genome size of P. armeniaca was only 2.33%, and there was low intraspecies variation among the studied groups; the genome size of P. armeniaca is highly stable.
- Published
- 2020
- Full Text
- View/download PDF
33. Genetic relationship between the ‘Korla fragrant pear’ and local pear varieties in xinjiang based on floral organ characteristics
- Author
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Kang Liao, Yingying Niu, Shikui Zhang, Shirong Zhao, Weiquan Zhou, Shengli Dong, Mansur Nasir, Xiangying Chen, and Wenwen Li
- Subjects
0106 biological sciences ,0301 basic medicine ,Pyrus ussuriensis ,PEAR ,biology ,Stamen ,Horticulture ,biology.organism_classification ,medicine.disease_cause ,01 natural sciences ,03 medical and health sciences ,030104 developmental biology ,Inflorescence ,Germination ,Pyrus × sinkiangensis ,Pollen ,medicine ,010606 plant biology & botany ,Pyrus communis - Abstract
The characters of floral organ and pollen of local pear varieties in Xinjiang, such as the ‘Korla fragrant pear’ and 39 other varieties or types, Pyrus bretschneideri Rehd., Pyrus pyrifolia Nakai., and Pyrus communis L. were measured and described. The genetic relationships between the ‘Korla fragrant pear’ and other local pear varieties in Xinjiang were analyzed by clustering. The number of flowers per inflorescence of varieties or types was approximately 7.6, and the difference in the number of stamens was small. Except for ‘Aiwenqieke’, ‘Qipanli’ and ‘Daxiangshui’, which had no pollen, the pollen quantity of the varieties or types was 1000.0–5000.0 grains. The pollen grains were isopolar and radiation symmetrical single-grain. The pollen grains had three germination furrows, and the main type of pollen grain exine ornamentation was stripe ornamentation. According to Erdtman’s NPC classification system, the pear pollen was of the N3P4C5 type. According to the clustering results, there was high similarity between the ‘Korla fragrant pear’ and Pyrus sinkiangensis Yu. The ‘Korla fragrant pear’ clustered with most local varieties in Xinjiang, and a genetic relationship between P. bretschneideri and P. pyrifolia was observed. There were interspecific and variety or type differences in floral organ and pollen morphology between P. sinkiangensis and P. bretschneideri, P. pyrifolia, Pyrus ussuriensis Maxim., P. communis and Pyrus betulifolia Bunge. There were many similarities between the ‘Korla fragrant pear’ and local pear varieties in Xinjiang, which should be classified within the P. sinkiangensis system.
- Published
- 2019
- Full Text
- View/download PDF
34. Impacts on wetlands of large-scale land use changes by agricultural development: the small Sanjiang plain, China
- Author
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Hongyu Liu, Shikui Zhang, Zhaofu Li, Xianguo Lu, and Qing Yang
- Subjects
Wetlands -- Protection and preservation ,Environmental issues - Abstract
An attempt is made to focus on degraded wetlands in the small Sanjiang plain in China, where waterfowls, fish and plants are found in abundance. The loss of these wetlands due to agricultural development has caused a loss of a number of habitats.
- Published
- 2004
35. The effect of siRNA-mediated lymphocyte-specific protein tyrosine kinase (Lck) inhibition on pulmonary inflammation in a mouse model of asthma
- Author
-
Shikui, Zhang, Rongjia, Yang, and Yonghua, Zheng
- Subjects
chemical and pharmacologic phenomena ,hemic and immune systems ,Original Article ,respiratory system ,respiratory tract diseases - Abstract
Objective: To explore the effect of siRNA-mediated inhibition of lymphocyte-specific protein tyrosine kinase (Lck) on pulmonary inflammation in a mouse model of asthma. Methods: A total of 32 female BABL/c mice were used in the study. The mouse asthma model was established with ovabumin (OVA), and Lck specific siRNA or nonspecific siRNA was transfected through the tail vein before the first OVA challenge. Two days after the last challenge, mice were sacrificed and bronchoalveolar lavage fluid (BALF), plasma and lung tissue were collected. Levels of Lck mRNA and protein in lung were detected by quantitative real-time PCR and western blot. The levels of IL-4 and IgE in BALF and plasma were detected with ELISA. Results: Lck specific siRNA significantly inhibited expression of Lck mRNA and protein in T cells. In vivo transfection of Lck siRNA down regulated the expression of Lck mRNA and protein in lung parenchymal homogenates. Sensitized mice treated with Lck siRNA prior to OVA challenge had fewer eosinophils in BALF and in lung sections and lower levels of IL-4 and IgE in BALF and plasma compared to those treated with nonspecific siRNA. Conclusions: Pretreatment of OVA sensitized mice with Lck siRNA results in attenuation of pulmonary inflammation following OVA challenge. Inhibition of Lck gene expression should be investigated further as a potential therapy for asthma.
- Published
- 2015
36. Impacts on Wetlands of Large-scale Land-use Changes by Agricultural Development: The Small Sanjiang Plain, China
- Author
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Xianguo Lu, Hong-Yu Liu, Qing Yang, Shikui Zhang, and Zhaofu Li
- Subjects
China ,Conservation of Natural Resources ,Floodplain ,Population Dynamics ,Geography, Planning and Development ,Biodiversity ,Wetland ,Sanjiang Plain ,Birds ,Rivers ,Water Supply ,Environmental protection ,Waterfowl ,Animals ,Environmental Chemistry ,Ecosystem ,geography ,geography.geographical_feature_category ,Ecology ,biology ,Land use ,Flooding (psychology) ,Agriculture ,General Medicine ,Plants ,biology.organism_classification ,Geographic Information Systems ,Environmental science ,Surface runoff ,Environmental Monitoring - Abstract
The Small Sanjiang Plain (SSP), was formerly the largest wetland complex in China, located in the Northeastern part of Heilongjiang Province, China. Home to vast numbers of waterfowls, fish, and plants, the SSP is globally significant for biodiversity conservation. The loss and fragmentation of wetlands as a result agricultural development over 50 years has impacted wetland communities and their biodiversity. We used GIS to inventory large-scale land-use changes from 1950 to 2000, together with other statistical data. We found that 73.6% of the wetlands were lost due to agricultural development. Consequences of these land-use changes included: i) a rapid decline in waterfowl and plant species with the loss and fragmentation of natural wetlands and wetland ecosystem degradation; ii) greater variation in wetland water levels as the result of land-use changes over the years; iii) disruption of the dynamic river-floodplain connection by construction of drainage ditches and levees; and iv) a decrease in floodplain area that caused increased flooding peak flows and runoff. Here we show how these changes affect wetland biodiversity and impact important wetland species.
- Published
- 2004
- Full Text
- View/download PDF
37. [Fragmentation process of wetland landscape in watersheds of Sanjiang Plain, China]
- Author
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Hongyu, Liu, Xianguo, Lü, Shikui, Zhang, and Qing, Yang
- Subjects
Conservation of Natural Resources ,Rivers ,Agriculture ,Ecosystem ,Environmental Monitoring - Abstract
The Sanjiang Plain is the largest fresh water wetland distribution area in China and the center of waterfowls breeding and habitat area in Asia, but over the past 50 years, more than 73% of its wetland had lost because of agricultural development, and as a result, the wetland biodiversity declines dramatically, and the remnant wetlands are in a very fragment state. Based on historical maps, remote sensing data and GIS techniques, this paper selected two watersheds to analyze their wetland landscape fragmentation process during 1950-2000. It was indicated that land reclamation resulted in a decrease of 98% wetland corridors in Qixing River, 90% in Naoli River, 87% in the middle reach of Bielahong River, and 94% in the lower reach of Bielahong River; The amount of isolated wetlands in watershed increased dramatically; The maximum patch areas of wetland decreased by 92.6% in Naoli River watershed and 74.6% in Bielahong River watershed, and the mean wetland patch area in the two watersheds decreased by 99%. Before 1983, the wetland landscape was in an extensive area distribution state (the index of patch density was0.1), but after 1983, it fragmented dramatically, with the index of patch density larger than 1.5. The shape fragmentation indices of wetland decreased from 1950 to 2000, indicating a very big change in wetland patch shapes in the watersheds. The area fragmentation indices of wetland also increased from 1950 to 2000, especially after 1983, showing that the wetlands were in a serious fragmentation state. The wetland landscape fragmentation changed from a landmass and island model to a satellite model, and finally to a completely isolated model, which indicated the great changes in spatial structure of wetland in the Sanjiang Plain.
- Published
- 2005
38. Yellow substance in Jiaozhou Bay of China and the prospect of its applications
- Author
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Jianmin Zheng, Yongsen Wu, Shikui Zhang, Xuqin Zhang, and Baohua Li
- Subjects
Salinity ,Pollutant ,Linear relationship ,Environmental chemistry ,Optical property ,Environmental science ,Seawater ,Water quality ,Absorption (electromagnetic radiation) ,Bay - Abstract
The optical property and bio-chemical composition of yellow substance in Jiaozhou Bay had been measured in a laboratory where the distribution of absorption coefficients of yellow substance in Jiaozhou Bay and near Qingdao City, that of fluorescence properties as well as that of the bio-chemical components contained in the water samples of yellow substance had obtained. From the data analysis, it is found that the absorption coefficient of yellow substance has a better linear relationship with salinity of the seawater, and is a better indicator for showing the environmental pollutants, water quality and the micro-construction of current field. These results will provide the reference for further searching the localization of satellite algorithms of yellow substance in China waters, monitoring environmental quality and applying to oceanographic research in the future.
- Published
- 2003
- Full Text
- View/download PDF
39. Flowering Biology and Fruit Development Characteristics of Apricot Cultivar 'Kezilang' in Xinjiang.
- Author
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Shengli DONG, Chaohai LI, Shikui ZHANG, NIYAZ, Ablah, and Runqing DU
- Published
- 2016
40. Differences in Light Response Curve and CO2 Response Curve of Korla Fragrant Pear Trees in Different Training Systems.
- Author
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Zhenbin JIANG, Kang LIAO, MANSUER, Nasi'er, Yingying NIU, Hongxiang PANG, Qi SUN, Le XU, and Shikui ZHANG
- Published
- 2016
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