79 results on '"Shihui Huang"'
Search Results
2. Genome-wide identification of copy number variations in wrinkled skin cases of Xiang pigs
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Xiaoli Liu, Fenbin Hu, Wei Wang, Xia Chen, Xi Niu, Shihui Huang, Zhou Wang, Jiafu Wang, and Xueqin Ran
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Xiang pig ,Wrinkled skin cases ,Copy number variation ,Aging ,Medicine ,Science - Abstract
Abstract Copy number variation (CNV) tends to occur in genetically enriched regions and is likely associated with a number of complex diseases such as skin aging. In this study, we investigated the genome-wide CNVs in 20 wrinkled skin cases (WSC) of Xiang pigs and 63 controls, and identified 7893 copy number variable regions (CNVRs). We estimated the F-statistic (Fst) at each locus and identified that 93 case-controls stratified CNVRs (Fst > = 0.15) overlapped with 87 known genes. Functional enrichment analysis showed that most of these genes were predominantly enriched in pathways and terms related to the extracellular matrix. Finally, we found that some CNVs were predicted to have high effects on genes such as VCAN, TIMP1 and FOXO1 through transcriptional amplification, transcript ablation and so on. Most of the genes overlapped with those CNVRs have been reported to be related to aging in human or animals. The copy numbers presented the positive correlations with the transcript level of the genes in skins between the cases and controls. Our results suggested that those 22 CNVRs, including 19 CNV losses and 3 CNV gains, were putatively associated with the skin wrinkle of Xiang pigs.
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- 2024
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3. The effects of meteorological factors and air pollutants on the incidence of tuberculosis in people living with HIV/AIDS in subtropical Guangxi, China
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Fengyi Wang, Zongxiang Yuan, Shanfang Qin, Fengxiang Qin, Junhan Zhang, Chuye Mo, Yiwen Kang, Shihui Huang, Fang Qin, Junjun Jiang, Aimei Liu, Hao Liang, and Li Ye
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DLNM ,Tuberculosis ,HIV/AIDS ,Meteorological factors ,Air Pollutant ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background Previous studies have shown the association between tuberculosis (TB) and meteorological factors/air pollutants. However, little information is available for people living with HIV/AIDS (PLWHA), who are highly susceptible to TB. Method Data regarding TB cases in PLWHA from 2014 to2020 were collected from the HIV antiviral therapy cohort in Guangxi, China. Meteorological and air pollutants data for the same period were obtained from the China Meteorological Science Data Sharing Service Network and Department of Ecology and Environment of Guangxi. A distribution lag non-linear model (DLNM) was used to evaluate the effects of meteorological factors and air pollutant exposure on the risk of TB in PLWHA. Results A total of 2087 new or re-active TB cases were collected, which had a significant seasonal and periodic distribution. Compared with the median values, the maximum cumulative relative risk (RR) for TB in PLWHA was 0.663 (95% confidence interval [CI]: 0.507–0.866, lag 4 weeks) for a 5-unit increase in temperature, and 1.478 (95% CI: 1.116–1.957, lag 4 weeks) for a 2-unit increase in precipitation. However, neither wind speed nor PM10 had a significant cumulative lag effect. Extreme analysis demonstrated that the hot effect (RR = 0.638, 95%CI: 0.425–0.958, lag 4 weeks), the rainy effect (RR = 0.285, 95%CI: 0.135–0.599, lag 4 weeks), and the rainless effect (RR = 0.552, 95%CI: 0.322–0.947, lag 4 weeks) reduced the risk of TB. Furthermore, in the CD4(+) T cells
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- 2024
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4. Comparative transcriptome analysis of longissimus dorsi muscle reveal potential genes affecting meat trait in Chinese indigenous Xiang pig
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Wei Wang, Dan Wang, Xinyi Zhang, Xiaoli Liu, Xi Niu, Sheng Li, Shihui Huang, Xueqin Ran, and Jiafu Wang
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Medicine ,Science - Abstract
Abstract In this study, we compared the transcriptome of longissimus dorsi muscle between Guizhou Xiang pigs (XP) and Western commercial Large White pigs (LW), which show diffirent meat quality between them. In terms of meat quality traits, the pH 45 min, color score, backfat thickness, and intramuscular fat (IMF) content were higher in Xiang pigs than in Large White pigs (P
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- 2024
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5. Assessing causal association of circulating micronutrients and systemic lupus erythematosus susceptibility: a Mendelian randomization study
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Shihui Huang, Xuemei Wei, Fang Qin, Zongxiang Yuan, Chuye Mo, Yiwen Kang, Chunlin Huang, Junjun Jiang, and Li Ye
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circulating micronutrients ,minerals ,vitamins ,systemic lupus erythematosus ,Mendelian randomization ,Nutrition. Foods and food supply ,TX341-641 - Abstract
BackgroundPrevious studies showed the conflicting associations between circulating micronutrient levels and systemic lupus erythematosus (SLE). Therefore, we aimed to clarify the causal association between circulating micronutrient levels and the risk of SLE by two-sample Mendelian randomization (MR) analysis.Methods56 single nucleotide polymorphisms (SNPs) significantly associated with 14 circulating micronutrients (vitamin A, B6, B9, B12, C, D and E, phosphorus, calcium, magnesium, copper, iron, zinc, and selenium) in published genome-wide association studies (GWAS) were used as instrumental variables (IVs). And summary statistics related to SLE were obtained from the IEU OpenGWAS database. We used the MR Steiger test to estimate the possible causal direction between circulating micronutrients and SLE. In the MR analysis, inverse variance weighting (IVW) method and the Wald ratio was as the main methods., Moreover, the MR-Pleiotropy residuals and outliers method (MR-PRESSO), Cochrane’s Q-test, MR-Egger intercept method and leave-one-out analyses were applied as sensitivity analyses. Additionally, we conducted a retrospective analysis involving the 20,045 participants from the Third National Health and Nutritional Examination Survey (NHANES III). Weight variables were provided in the NHANES data files. Univariate and multivariate logistic regression analyses were performed to determine the associations between circulating micronutrients and SLE.ResultsThe MR estimates obtained from the IVW method revealed potential negative correlations between circulating calcium (OR: 0.06, 95% CI: 0.01–0.49, P = 0.009), iron levels (OR: 0.63, 95% CI: 0.43–0.92, P = 0.016) and the risk of SLE. The results remained robust, even under various pairs of sensitivity analyses. Our retrospective analysis demonstrated that the levels of vitamin D, serum total calcium, and serum iron were significantly lower in SLE patients (N = 40) when compared to the control group (N = 20,005). Multivariate logistic regression analysis further established that increased levels of vitamin D and serum total calcium served as protective factors against SLE.ConclusionOur results provided genetic evidence supporting the potential protective role of increasing circulating calcium in the risk of SLE. Maintaining adequate levels of calcium may help reduce the risk of SLE.
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- 2024
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6. Identification of genomic characteristics and selective signals in Guizhou black goat
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Lingle Chang, Yundi Zheng, Sheng Li, Xi Niu, Shihui Huang, Qingmeng Long, Xueqin Ran, and Jiafu Wang
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Guizhou black goat ,Whole-genome sequencing ,Genetic diversity ,Selection signature ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Guizhou black goat is one of the indigenous black goat breeds in the southwest region of Guizhou, China, which is an ordinary goat for mutton production. They are characterized by moderate body size, black coat, favorite meat quality with tender meat and lower odor, and tolerance for cold and crude feed. However, little is known about the genetic characteristics or variations underlying their important economic traits. Results Here, we resequenced the whole genome of Guizhou black goat from 30 unrelated individuals breeding in the five core farms. A total of 9,835,610 SNPs were detected, and 2,178,818 SNPs were identified specifically in this breed. The population structure analysis revealed that Guizhou black goat shared a common ancestry with Shaanbei white cashmere goat (0.146), Yunshang black goat (0.103), Iran indigenous goat (0.054), and Moroccan goat (0.002). However, Guizhou black goat showed relatively higher genetic diversity and a lower level of linkage disequilibrium than the other seven goat breeds by the analysis of the nucleotide diversity, linkage disequilibrium decay, and runs of homozygosity. Based on F ST and θπ values, we identified 645, 813, and 804 selected regions between Guizhou black goat and Yunshang black goat, Iran indigenous goat, and cashmere goats. Combined with the results of XP-EHH, there were 286, 322, and 359 candidate genes, respectively. Functional annotation analysis revealed that these genes are potentially responsible for the immune response (e.g., CD28, CD274, IL1A, TLR2, and SLC25A31), humility-cold resistance (e.g., HBEGF, SOSTDC1, ARNT, COL4A1/2, and EP300), meat quality traits (e.g., CHUK, GAB2, PLAAT3, and EP300), growth (e.g., GAB2, DPYD, and CSF1), fertility (e.g., METTL15 and MEI1), and visual function (e.g., PANK2 and NMNAT2) in Guizhou black goat. Conclusion Our results indicated that Guizhou black goat had a high level of genomic diversity and a low level of linkage disequilibrium in the whole genome. Selection signatures were detected in the genomic regions that were mainly related to growth and development, meat quality, reproduction, disease resistance, and humidity-cold resistance in Guizhou black goat. These results would provide a basis for further resource protection and breeding improvement of this very local breed.
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- 2024
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7. Causal relationship between gut microbiota and tuberculosis: a bidirectional two-sample Mendelian randomization analysis
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Zongxiang Yuan, Yiwen Kang, Chuye Mo, Shihui Huang, Fang Qin, Junhan Zhang, Fengyi Wang, Junjun Jiang, Xiaoxiang Yang, Hao Liang, and Li Ye
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Gut microbiota ,Tuberculosis ,Respiratory tuberculosis ,Extrapulmonary tuberculosis ,Mendelian randomization ,Causal relationship ,Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background Growing evidence from observational studies and clinical trials suggests that the gut microbiota is associated with tuberculosis (TB). However, it is unclear whether any causal relationship exists between them and whether causality is bidirectional. Methods A bidirectional two-sample Mendelian randomization (MR) analysis was performed. The genome-wide association study (GWAS) summary statistics of gut microbiota were obtained from the MiBioGen consortium, while the GWAS summary statistics of TB and its specific phenotypes [respiratory tuberculosis (RTB) and extrapulmonary tuberculosis (EPTB)] were retrieved from the UK Biobank and the FinnGen consortium. And 195 bacterial taxa from phylum to genus were analyzed. Inverse variance weighted (IVW), MR-Egger regression, maximum likelihood (ML), weighted median, and weighted mode methods were applied to the MR analysis. The robustness of causal estimation was tested using the heterogeneity test, horizontal pleiotropy test, and leave-one-out method. Results In the UK Biobank database, we found that 11 bacterial taxa had potential causal effects on TB. Three bacterial taxa genus.Akkermansia, family.Verrucomicrobiacea, order.Verrucomicrobiales were validated in the FinnGen database. Based on the results in the FinnGen database, the present study found significant differences in the characteristics of gut microbial distribution between RTB and EPTB. Four bacterial taxa genus.LachnospiraceaeUCG010, genus.Parabacteroides, genus.RuminococcaceaeUCG011, and order.Bacillales were common traits in relation to both RTB and TB, among which order.Bacillales showed a protective effect. Additionally, family.Bacteroidacea and genus.Bacteroides were identified as common traits in relation to both EPTB and TB, positively associating with a higher risk of EPTB. In reverse MR analysis, no causal association was identified. No significant heterogeneity of instrumental variables (IVs) or horizontal pleiotropy was found. Conclusion Our study supports a one-way causal relationship between gut microbiota and TB, with gut microbiota having a causal effect on TB. The identification of characteristic gut microbiota provides scientific insights for the potential application of the gut microbiota as a preventive, diagnostic, and therapeutic tool for TB.
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- 2024
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8. Genome-wide characterization of structure variations in the Xiang pig for genetic resistance to African swine fever
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Fenfang Qi, Xia Chen, Jiafu Wang, Xi Niu, Sheng Li, Shihui Huang, and Xueqin Ran
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Structural variation ,genetic resistance ,African swine fever ,Xiang pig ,Infectious and parasitic diseases ,RC109-216 - Abstract
African swine fever (ASF) is a rapidly fatal viral haemorrhagic fever in Chinese domestic pigs. Although very high mortality is observed in pig farms after an ASF outbreak, clinically healthy and antibody-positive pigs are found in those farms, and viral detection is rare from these pigs. The ability of pigs to resist ASF viral infection may be modulated by host genetic variations. However, the genetic basis of the resistance of domestic pigs against ASF remains unclear. We generated a comprehensive set of structural variations (SVs) in a Chinese indigenous Xiang pig with ASF-resistant (Xiang-R) and ASF-susceptible (Xiang-S) phenotypes using whole-genome resequencing method. A total of 53,589 nonredundant SVs were identified, with an average of 25,656 SVs per individual in the Xiang pig genome, including insertion, deletion, inversion and duplication variations. The Xiang-R group harboured more SVs than the Xiang-S group. The F-statistics (FST) was carried out to reveal genetic differences between two populations using the resequencing data at each SV locus. We identified 2,414 population-stratified SVs and annotated 1,152 Ensembl genes (including 986 protein-coding genes), in which 1,326 SVs might disturb the structure and expression of the Ensembl genes. Those protein-coding genes were mainly enriched in the Wnt, Hippo, and calcium signalling pathways. Other important pathways associated with the ASF viral infection were also identified, such as the endocytosis, apoptosis, focal adhesion, Fc gamma R-mediated phagocytosis, junction, NOD-like receptor, PI3K-Akt, and c-type lectin receptor signalling pathways. Finally, we identified 135 candidate adaptive genes overlapping 166 SVs that were involved in the virus entry and virus-host cell interactions. The fact that some of population-stratified SVs regions detected as selective sweep signals gave another support for the genetic variations affecting pig resistance against ASF. The research indicates that SVs play an important role in the evolutionary processes of Xiang pig adaptation to ASF infection.
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- 2024
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9. The global burden of cardiovascular diseases and type 2 diabetes attributable to low physical activity, 1990–2019: an analysis from the global burden of disease study
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Junhan Zhang, Zongxiang Yuan, Chuye Mo, Yiwen Kang, Fengyi Wang, Xueqin Wei, Shihui Huang, Fang Qin, Junjun Jiang, Hao Liang, and Li Ye
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cardiovascular diseases ,type 2 diabetes ,low physical activity ,global burden of diseases ,epidemiology ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
BackgroundCardiovascular diseases (CVD) and type 2 diabetes (T2D) account for the majority of the burden of noncommunicable disease caused by low physical activity (LPA). In order to inform future interventions, this study aims to assess the burden and trends in mortality and disability-adjusted life years (DALYs) of CVD and T2D attributable to LPA by year, location, sex, and age from 1990 to 2019.MethodsMortality, DALYs, and their age-standardised rates (ASMR, ASDR) for CVD and T2D attributable to LPA were retrieved from Global Burden of Disease (GBD) 2019. The estimated annual percentage changes (EAPCs) were calculated using linear regression model to describe the trend over time.ResultsFrom 1990 to 2019, the number of deaths caused by both CVD and T2D due to LPA increased significantly globally. However, the overall ASMR and ASDR for CVD declined over this same period [EAPC for ASMR (CVD) = −1.44 (95% CI: −1.50–1.38), EAPC for ASDR (CVD) = −1.30 (95% CI: −1.35 to −1.25)]. In terms of disparities, ASMR (CVD) and ASDR (CVD) in North Africa and the Middle East were consistently higher than the global average; also, the sex difference in ASMR was greatest in Central Asia. ASMR among people aged 25–44 in high Socio-Demographic Index (SDI) region has increased significantly over the past three decades. ASMR (T2D) due to LPA showed an increasing trend year by year, with EAPC = 0.26 (95% CI: 0.13–0.39), and this rate increased faster in males than in females. Consistent with cardiovascular diseases, ASMR of type 2 diabetes attributable to LPA increased among people aged 25–44, while decreased in other age groups in high SDI region.ConclusionInterventions targeting LPA are warranted in controlling the burden of cardiovascular diseases and type 2 diabetes. Countries should adapt strategies to their local contexts, considering the sex and age differences among their populations. The 25–44 age group should be given special attention to prevent the disease burden from worsening among younger people.
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- 2023
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10. Whole-genome sequence analysis reveals selection signatures for important economic traits in Xiang pigs
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Xiying Wang, Xueqin Ran, Xi Niu, Shihui Huang, Sheng Li, and Jiafu Wang
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Medicine ,Science - Abstract
Abstract Xiang pig (XP) is one of the best-known indigenous pig breeds in China, which is characterized by its small body size, strong disease resistance, high adaptability, favorite meat quality, small litter sizes, and early sexual maturity. However, the genomic evidence that links these unique traits of XP is still poorly understood. To identify the genomic signatures of selection in XP, we performed whole-genome resequencing on 25 unrelated individual XPs. We obtained 876.70 Gb of raw data from the genomic libraries. The LD analysis showed that the lowest level of linkage disequilibrium was observed in Xiang pig. Comparative genomic analysis between XPs and other breeds including Tibetan, Meishan, Duroc and Landrace revealed 3062, 1228, 907 and 1519 selected regions, respectively. The genes identified in selected regions of XPs were associated with growth and development processes (IGF1R, PROP1, TBX19, STAC3, RLF, SELENOM, MSTN), immunity and disease resistance (ZCCHC2, SERPINB2, ADGRE5, CYP7B1, STAT6, IL2, CD80, RHBDD3, PIK3IP1), environmental adaptation (NR2E1, SERPINB8, SERPINB10, SLC26A7, MYO1A, SDR9C7, UVSSA, EXPH5, VEGFC, PDE1A), reproduction (CCNB2, TRPM6, EYA3, CYP7B1, LIMK2, RSPO1, ADAM32, SPAG16), meat quality traits (DECR1, EWSR1), and early sexual maturity (TAC3). Through the absolute allele frequency difference (ΔAF) analysis, we explored two population-specific missense mutations occurred in NR6A1 and LTBP2 genes, which well explained that the vertebrae numbers of Xiang pigs were less than that of the European pig breeds. Our results indicated that Xiang pigs were less affected by artificial selection than the European and Meishan pig breeds. The selected candidate genes were mainly involved in growth and development, disease resistance, reproduction, meat quality, and early sexual maturity. This study provided a list of functional candidate genes, as well as a number of genetic variants, which would provide insight into the molecular basis for the unique traits of Xiang pig.
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- 2022
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11. CircRNAs in Xiang pig ovaries among diestrus and estrus stages
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Xi Niu, Yali Huang, Huan Lu, Sheng Li, Shihui Huang, Xueqin Ran, and Jiafu Wang
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Xiang pig ,Ovary ,Estrus ,CircRNA ,Expression profile ,CeRNA network ,Animal culture ,SF1-1100 ,Veterinary medicine ,SF600-1100 - Abstract
Abstract Background The fecundity of sows is a trait of major economic in pig industry. The molecular regulation of estrus cycles can affect the fecundity of female animals. Compared with the other pig breeds, Xiang pig exhibits the special estrus behaviors. CircRNAs are thought to involve in regulation of multiple biological processes. However, the potential roles of circRNAs in ovary regulation on Xiang pig estrus are largely unknown. Results 8,937 circRNAs were identified from eight libraries constructed from the ovarian samples of Xiang pig at estrus and diestrus stages by RNA sequencing method. Of which, 1,995 were high confidence circRNAs detected at least two junction reads in each ovary sample and seven circRNAs were validated by RT-PCR method. Furthermore, we identified 290 upregulated and 15 downregulated circRNAs in estrus ovaries. These differentially expressed circRNAs (DECs) derived from 273 host genes. And 207 miRNAs were identified to be targets sponged by 156 DECs with 432 binding sites, containing more than one miRNA binding site in each circRNA. Function enrichment analysis revealed that the host genes and the targets of miRNAs sponged by DECs were enriched in several reproduction-related signaling pathways, such as ovarian steroidogenesis, oocyte maturation, circadian rhythm, estrogen signaling pathway, GnRH signaling pathway, circadian entrainment, and oocyte meiosis. The circRNA-miRNA-mRNA networks revealed that 153 miRNAs interacting with 122 DECs and 86 miRNAs interacting with 84 DECs were involved in ovarian functions and ovarian circadian entrainment and circadian rhythm respectively. The DEC-miRNA-DEG (differentially expressed gene, DEG) networks associated with reproduction-related signaling pathways contained 22 DECs,18 miRNAs and 7 DEGs. 22 DECs were recognized as hub circRNAs during the estrus phase of Xiang pigs. Conclusions The circRNAs that function as miRNA sponges could play a key role in post-transcriptional regulation of gene expression during Xiang pig’s estrus cycle.
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- 2022
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12. 500 metagenome-assembled microbial genomes from 30 subtropical estuaries in South China
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Lei Zhou, Shihui Huang, Jiayi Gong, Peng Xu, and Xiande Huang
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Science - Abstract
Measurement(s) Metagenome-assembled genomes Technology Type(s) Metagenomics Sample Characteristic - Organism Bacteria • Archaea Sample Characteristic - Environment estuarine water Sample Characteristic - Location South China Sea coastal waters of the mainland of China
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- 2022
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13. Support Vector Regression Based on the Particle Swarm Optimization Algorithm for Tight Oil Recovery Prediction
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Shihui Huang, Leng Tian, Jinshui Zhang, Xiaolong Chai, Hengli Wang, and Hongling Zhang
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Chemistry ,QD1-999 - Published
- 2021
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14. Differences in gene expression and variable splicing events of ovaries between large and small litter size in Chinese Xiang pigs
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Xueqin Ran, Fengbin Hu, Ning Mao, Yiqi Ruan, Fanli Yi, Xi Niu, Shihui Huang, Sheng Li, Longjiang You, Fuping Zhang, Liangting Tang, Jiafu Wang, and Jianfeng Liu
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Transcriptome ,Alternative splicing ,Ovary ,Litter size ,Xiang pig ,Animal culture ,SF1-1100 ,Veterinary medicine ,SF600-1100 - Abstract
Abstract Background Although lots of quantitative trait loci (QTLs) and genes present roles in litter size of some breeds, the information might not make it clear for the huge diversity of reproductive capability in pig breeds. To elucidate the inherent mechanisms of heterogeneity of reproductive capability in litter size of Xiang pig, we performed transcriptome analysis for the expression profile in ovaries using RNA-seq method. Results We identified 1,419 up-regulated and 1,376 down-regulated genes in Xiang pigs with large litter size. Among them, 1,010 differentially expressed genes (DEGs) were differently spliced between two groups with large or small litter sizes. Based on GO and KEGG analysis, numerous members of genes were gathered in ovarian steroidogenesis, steroid biosynthesis, oocyte maturation and reproduction processes. Conclusions Combined with gene biological function, twelve genes were found out that might be related with the reproductive capability of Xiang pig, of which, eleven genes were recognized as hub genes. These genes may play a role in promoting litter size by elevating steroid and peptide hormones supply through the ovary and facilitating the processes of ovulation and in vivo fertilization.
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- 2021
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15. miR-103-3p Regulates the Differentiation and Autophagy of Myoblasts by Targeting MAP4
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Xianxian Zhang, Shihui Huang, Xi Niu, Sheng Li, Jiafu Wang, and Xueqin Ran
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miR-103-3p ,differentiation ,autophagy ,MAP4 ,skeletal muscle ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Skeletal muscle is the most abundant tissue in mammals, and myogenesis and differentiation require a series of regulatory factors such as microRNAs (miRNAs). In this study, we found that miR-103-3p was highly expressed in the skeletal muscle of mice, and the effects of miR-103-3p on skeletal muscle development were explored using myoblast C2C12 cells as a model. The results showed that miR-103-3p could significantly reduce myotube formation and restrain the differentiation of C2C12 cells. Additionally, miR-103-3p obviously prevented the production of autolysosomes and inhibited the autophagy of C2C12 cells. Moreover, bioinformatics prediction and dual-luciferase reporter assays confirmed that miR-103-3p could directly target the microtubule-associated protein 4 (MAP4) gene. The effects of MAP4 on the differentiation and autophagy of myoblasts were then elucidated. MAP4 promoted both the differentiation and autophagy of C2C12 cells, which was contrary to the role of miR-103-3p. Further research revealed that MAP4 colocalized with LC3 in C2C12 cell cytoplasm, and the immunoprecipitation assay showed that MAP4 interacted with autophagy marker LC3 to regulate the autophagy of C2C12 cells. Overall, these results indicated that miR-103-3p regulated the differentiation and autophagy of myoblasts by targeting MAP4. These findings enrich the understanding of the regulatory network of miRNAs involved in the myogenesis of skeletal muscle.
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- 2023
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16. Product Innovation Design Method Based on BP Neural Network
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Shihui Huang
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Electronic computers. Computer science ,QA75.5-76.95 - Abstract
Innovative product design is the core problem of modern industry and the source power of enterprise development. With the continuous improvement of China’s material level, product innovation design has changed from functional needs to emotional needs, and the product design method centered on users’ emotional needs has attracted much attention. With the emergence of new technologies such as artificial intelligence and big data, the automation and intelligence of advanced algorithms and innovative design methods for online product decision driven by multimodal multimedia data in e-commerce have become inevitable trends in the field of product design, and it is a big challenge to improve the innovative design ability of products by using information technology. In order to solve this problem, based on the research of BP neural network technology, this paper puts forward an innovative product design method based on image-Kempi method. The color features of images are reconstructed and integrated, and then transferred to product modeling, so as to generate new products with both content image modeling features and style image color features, which brings visual inspiration to users. The results show that KENPI method combines the theories of Kansei engineering, convolutional neural network, and neural style transfer, and establishes a mapping model between product modeling elements and product semantics. Convolutional neural network and neural style transfer model are used to extract, reconstruct, and integrate the color features of style diagrams, and transfer them to product modeling to design new product images. By evaluating the image quality and comparing the product semantics before and after the migration, the validity and feasibility of the migration are verified. Based on BP neural network, a nonlinear mapping model between product attribute space and product semantic space is constructed, and the generalization ability of the model is evaluated, which verifies the feasibility and effectiveness of this method. The research results have important theoretical guiding significance for improving the innovative design ability of enterprises, enhancing product competitiveness, and customer satisfaction.
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- 2022
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17. Impact of the Aversive Effects of Drugs on Their Use and Abuse
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Anthony L. Riley, Hayley N. Manke, and Shihui Huang
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Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Drug use and abuse are complex issues in that the basis of each may involve different determinants and consequences, and the transition from one to the other may be equally multifaceted. A recent model of the addiction cycle (as proposed by Koob and his colleagues) illustrates how drug-taking patterns transition from impulsive (acute use) to compulsive (chronic use) as a function of various neuroadaptations leading to the downregulation of DA systems, upregulation of stress systems, and the dysregulation of the prefrontal/orbitofrontal cortex. Although the nature of reinforcement in the initiation and mediation of these effects may differ (positive vs. negative), the role of reinforcement in drug intake (acute and chronic) is well characterized. However, drugs of abuse have other stimulus properties that may be important in their use and abuse. One such property is their aversive effects that limit drug intake instead of initiating and maintaining it. Evidence of such effects comes from both clinical and preclinical populations. In support of this position, the present review describes the aversive effects of drugs (assessed primarily in conditioned taste aversion learning), the fact that they occur concurrently with reward as assessed in combined taste aversion/place preference designs, the role of aversive effects in drug-taking (in balance with their rewarding effects), the dissociation of these affective properties in that they can be affected in different ways by the same manipulations, and the impact of various parametric, experiential, and subject factors on the aversive effects of drugs and the consequent impact of these factors on their use and abuse potential.
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- 2022
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18. Manganese Stress Adaptation Mechanisms of Bacillus safensis Strain ST7 From Mine Soil
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Xueqin Ran, Zhongmei Zhu, Hong Long, Qun Tian, Longjiang You, Xingdiao Wu, Qin Liu, Shihui Huang, Sheng Li, Xi Niu, and Jiafu Wang
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Bacillus safensis ,manganese oxidation ,transcriptome ,soil ,adaptation ,Microbiology ,QR1-502 - Abstract
The mechanism of bacterial adaption to manganese-polluted environments was explored using 50 manganese-tolerant strains of bacteria isolated from soil of the largest manganese mine in China. Efficiency of manganese removal by the isolated strains was investigated using atomic absorption spectrophotometry. Bacillus safensis strain ST7 was the most effective manganese-oxidizing bacteria among the tested isolates, achieving up to 82% removal at a Mn(II) concentration of 2,200 mg/L. Bacteria-mediated manganese oxide precipitates and high motility were observed, and the growth of strain ST7 was inhibited while its biofilm formation was promoted by the presence of Mn(II). In addition, strain ST7 could grow in the presence of high concentrations of Al(III), Cr(VI), and Fe(III). Genome-wide analysis of the gene expression profile of strain ST7 using the RNA-seq method revealed that 2,580 genes were differently expressed under Mn(II) exposure, and there were more downregulated genes (n = 2,021) than upregulated genes (n = 559) induced by Mn stress. KAAS analysis indicated that these differently expressed genes were mainly enriched in material metabolisms, cellular processes, organism systems, and genetic and environmental information processing pathways. A total of twenty-six genes from the transcriptome of strain ST7 were involved in lignocellulosic degradation. Furthermore, after 15 genes were knocked out by homologous recombination technology, it was observed that the transporters, multicopper oxidase, and proteins involved in sporulation and flagellogenesis contributed to the removal of Mn(II) in strain ST7. In summary, B. safensis ST7 adapted to Mn exposure by changing its metabolism, upregulating cation transporters, inhibiting sporulation and flagellogenesis, and activating an alternative stress-related sigB pathway. This bacterial strain could potentially be used to restore soil polluted by multiple heavy metals and is a candidate to support the consolidated bioprocessing community.
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- 2021
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19. Design, Synthesis and Biological Evaluation of Novel and Potent Protein Arginine Methyltransferases 5 Inhibitors for Cancer Therapy
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Yixuan Tang, Shihui Huang, Xingxing Chen, Junzhang Huang, Qianwen Lin, Lei Huang, Shuping Wang, Qihua Zhu, Yungen Xu, and Yi Zou
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epigenetic ,PRMT5 inhibitors ,THIQ ,cancer ,Organic chemistry ,QD241-441 - Abstract
Protein arginine methyltransferases 5 (PRMT5) is a clinically promising epigenetic target that is upregulated in a variety of tumors. Currently, there are several PRMT5 inhibitors under preclinical or clinical development, however the established clinical inhibitors show favorable toxicity. Thus, it remains an unmet need to discover novel and structurally diverse PRMT5 inhibitors with characterized therapeutic utility. Herein, a series of tetrahydroisoquinoline (THIQ) derivatives were designed and synthesized as PRMT5 inhibitors using GSK-3326595 as the lead compound. Among them, compound 20 (IC50: 4.2 nM) exhibits more potent PRMT5 inhibitory activity than GSK-3326595 (IC50: 9.2 nM). In addition, compound 20 shows high anti-proliferative effects on MV-4-11 and MDA-MB-468 tumor cells and low cytotoxicity on AML-12 hepatocytes. Furthermore, compound 20 possesses acceptable pharmacokinetic profiles and displays considerable in vivo antitumor efficacy in a MV-4-11 xenograft model. Taken together, compound 20 is an antitumor compound worthy of further study.
- Published
- 2022
- Full Text
- View/download PDF
20. Towards a tailored indoor horticulture: a functional genomics guided phenotypic approach
- Author
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Claudius Marondedze, Xinyun Liu, Shihui Huang, Cynthia Wong, Xuan Zhou, Xutong Pan, Huiting An, Nuo Xu, Xuechen Tian, and Aloysius Wong
- Subjects
Botany ,QK1-989 ,Plant culture ,SB1-1110 - Abstract
Indoor horticulture: Lighting the way to sustainability Tailored multidisciplinary approaches to hone sustainable indoor horticulture could significantly improve plant yields and crop quality. Advances in artificial lighting systems could transform commercial-scale indoor horticulture, but the current technology is limited by a lack of molecular data for plants grown under such lighting schemes. Aloysius Wong at Wenzhou-Kean University in Wenzhou, China, and co-workers reviewed research into the effects of light quality and differing wavelengths on plant growth. The team advocate the use of plant type-specific and functional genomics studies to examine light-determined molecular traits and associated gene expression. These could be used to build an extensive catalog of light qualities that enhance indoor crop yields and quality. Combining LED lights of different colors and wavelengths shows promise, and the researchers highlight the potential of tunable narrow wavelength lights, such as lasers.
- Published
- 2018
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21. Discovery of Novel Small-Molecule Inhibitors of PD-1/PD-L1 Interaction via Structural Simplification Strategy
- Author
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Hongbo Zhang, Yu Xia, Chunqiu Yu, Huijie Du, Jinchang Liu, Hui Li, Shihui Huang, Qihua Zhu, Yungen Xu, and Yi Zou
- Subjects
small molecule PD-1/PD-L1 inhibitor ,PD-1/PD-L1 ,structural simplification strategy ,Organic chemistry ,QD241-441 - Abstract
Blockade of the programmed cell death 1 (PD-1)/programmed cell death-ligand 1 (PD-L1) interaction is currently the focus in the field of cancer immunotherapy, and so far, several monoclonal antibodies (mAbs) have achieved encouraging outcomes in cancer treatment. Despite this achievement, mAbs-based therapies are struggling with limitations including poor tissue and tumor penetration, long half-life time, poor oral bioavailability, and expensive production costs, which prompted a shift towards the development of the small-molecule inhibitors of PD-1/PD-L1 pathways. Even though many small-molecule inhibitors targeting PD-1/PD-L1 interaction have been reported, their development lags behind the corresponding mAb, partly due to the challenges of developing drug-like small molecules. Herein, we report the discovery of a series of novel inhibitors targeting PD-1/PD-L1 interaction via structural simplification strategy by using BMS-1058 as a starting point. Among them, compound A9 stands out as the most promising candidate with excellent PD-L1 inhibitory activity (IC50 = 0.93 nM, LE = 0.43) and high binding affinity to hPD-L1 (KD = 3.64 nM, LE = 0.40). Furthermore, A9 can significantly promote the production of IFN-γ in a dose-dependent manner by rescuing PD-L1 mediated T-cell inhibition in Hep3B/OS-8/hPD-L1 and CD3-positive T cells co-culture assay. Taken together, these results suggest that A9 is a promising inhibitor of PD-1/PD-L1 interaction and is worthy for further study.
- Published
- 2021
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- View/download PDF
22. Characterization and phylogenetic analysis of the complete mitochondrial genome of Prolixicheilus longisulcus (Teleost: Cyprinidae)
- Author
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Lei Zhou, Shihui Huang, Tianxu Kuang, and Yusen Li
- Subjects
prolixicheilus longisulcus ,mitochondrial genome ,phylogenetic analysis ,Genetics ,QH426-470 - Abstract
In this study, we assembled the complete mitochondrial genome of Prolixicheilus longisulcus based on high-throughput Illumina sequencing. The complete mitochondrial genome of P. longisulcus was 16,598 bp in size. It was made up of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control region. The overall base composition included A(32.38%), T(26.08%), C(26.32%) and G(15.22%). The phylogenetic tree was conducted to provide a relationship within Labeoninae based on the maximum likelihood (ML) method. The ML tree demonstrated this species had the closest relationship with Cophecheilus bamen. These results should help to better understand the phylogenetic evolution of Labeoninae.
- Published
- 2020
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23. Voltage Flicker Detection Based on Probability Resampling
- Author
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Haitao Gao, Peng Xu, Jin Tao, Shihui Huang, Rugang Wang, and Quan Zhou
- Subjects
probability resampling ,flicker detection ,power quality ,voltage fluctuation ,data compression ,Technology - Abstract
Digital flicker detection devices need to store a large amount of evaluation data during measurement process, which leads to high requirements for hardware resources and algorithm execution efficiency. In this paper, a digital flicker detection method based on probability resampling is studied. In particular, before statistical evaluation, probability resampling is applied to screen the instantaneous flicker visual sensitivity data to compress redundant data. Additionally, the effectiveness of the method was numerically simulated and experimentally tested. The results show that the proposed method can accurately measure the voltage flicker value and can effectively compress the redundant evaluation data to be evaluated and has significant advantages in releasing hardware storage space, in improving algorithm execution efficiency and real-time performance, and in reducing processor workload. This method provides an engineering application reference for designing digital flicker detectors, especially for the software upgrade of traditional power quality testing equipment.
- Published
- 2020
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24. The global burden of cardiovascular diseases and type 2 diabetes attributable to low physical activity, 1990-2019: an analysis from the global burden of disease study.
- Author
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Junhan Zhang, Zongxiang Yuan, Chuye Mo, Yiwen Kang, Fengyi Wang, Xueqin Wei, Shihui Huang, Fang Qin, Junjun Jiang, Hao Liang, and Li Ye
- Published
- 2024
- Full Text
- View/download PDF
25. Sensilla on the labial palps of the cave species Tachycines plumiopedella Li, Feng & Luo, 2021 (Orthoptera: Rhaphidophoridae)
- Author
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Kesong Yin, Shihui Huang, and Changqing Luo
- Subjects
Animal Science and Zoology ,Developmental Biology - Published
- 2023
26. New taxonomic status of the Pseudotachycines procerus guizhouensis Zhu & Shi, 2022 (Rhaphidophoridae: Aemodogryllinae: Aemodogryllini)
- Author
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SHIHUI HUANG, CHUN LIN, and CHANGQING LUO
- Subjects
Animal Science and Zoology ,Ecology, Evolution, Behavior and Systematics - Abstract
The genus Pseudotachycines belongs to the tribe Aemodogryllini of the subfamily Aemodogryllinae. In the present paper, we examined the morphological features of male and female adults of the subspecies P. procerus guizhouensis, and suggested that this subspecies should be treated as a separate species. We redescribed the male of P. guizhouensis in detail. The morphological information and some related photographs of the female of this species were provided for the first time in this study. The morphological differentiation between P. guizhouensis and P. procerus was discussed.
- Published
- 2023
27. Description of a new species of the subgenus Tachycines (Tachycines) (Rhaphidophoridae: Aemodogryllinae: Aemodogryllini) from Guizhou, China
- Author
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BEN HONG, SHIHUI HUANG, and CHANGQING LUO
- Subjects
Insecta ,Arthropoda ,Rhaphidophoridae ,Animalia ,Orthoptera ,Animal Science and Zoology ,Biodiversity ,Ecology, Evolution, Behavior and Systematics ,Taxonomy - Abstract
In this study, we identify and describe a new species of the subgenus Tachycines (Tachycines), namely Tachycines (Tachycines) nigrocorporis sp. nov., which was collected from Guizhou Province, China, bringing the species number of this subgenus to twenty-two. The morphological differentiation between this new species and other species of Tachycines (Tachycines) is discussed. The morphological photographs of the new species are provided.
- Published
- 2022
28. Comparison of the caecal microbial community structure and physiological indicators of healthy and infection Eimeria tenella chickens during peak of oocyst shedding
- Author
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Bi Wang, Peng Du, Shihui Huang, Dan He, Jiaqi Chen, Xin Wen, Jian Yang, Simei Xian, and Zhentao Cheng
- Subjects
General Immunology and Microbiology ,Food Animals ,Animal Science and Zoology - Published
- 2022
29. The Effects of Photo Decoration Cues on Online Consumers’ Affective Responses
- Author
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Shihui Huang and Feng Zhou
- Abstract
This study refines three decoration cues from the photos in the online grocery store which enable consumer to experience grocery with affective responses. Three cues on consumers’ affective perception, attitudes as well as behavioral intention were examined in a laboratory experiment. The experiment results show that (1) both reflective surface and complementary goods layout lead to visual appeal and shopping enjoyment; (2) contrast color usage positively impacts on visual appeal while does not significantly affect shopping enjoyment; (3) consumers’ affective responses positively impact attitudes toward product and store which in turn lead to purchase intention and store loyalty. Implications for research and practice are discussed.
- Published
- 2022
30. Effects of Estrus Cycle on Expression of Ovarian Biological Clock-related Genes of Xiang Pig.
- Author
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Liangting TANG, Shihui HUANG, Xi NIU, Sheng LI, Jiafu WANG, and Xueqin RAN
- Subjects
MENSTRUAL cycle ,ESTRUS ,ALTERNATIVE RNA splicing ,CLOCK genes ,MOLECULAR clock ,GENE expression - Abstract
Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
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- View/download PDF
31. Conditioned taste avoidance and conditioned place preference induced by the third-generation synthetic cathinone eutylone in female sprague-dawley rats
- Author
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Robert A. Jones, Shihui Huang, Hayley N. Manke, and Anthony L. Riley
- Subjects
Pharmacology ,Psychiatry and Mental health ,Pharmacology (medical) - Published
- 2023
32. Novel evolutionary insights into nemacheilid cavefish: evidence from comparative analysis of mitochondrial genomes
- Author
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Lei Zhou, Shihui Huang, Qing Wang, Zhenhai Li, Zongyang Li, Anyou He, Jiehu Chen, Li Liu, and Keshu Zou
- Subjects
Oceanography ,Water Science and Technology - Published
- 2022
33. The effects of estrus cycle on the expression of ovarian biological clock-related genes of Xiang pigs
- Author
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Liang-Ting Tang, Shihui Huang, Xi Niu, Sheng Li, Jiafu Wang, and Xue-Qin Ran
- Abstract
The biological clock has been studied to play a critical role in the reproductive system of various living organisms like swine. To examine the effects of estrus cycle on the expression of ovarian biological clock-related gene in Xiang pig, in this study, we analyzed the expression and alternative splicing of biological clock-related genes in ovary during estrus and diestrus periods. In total, we detected 90 clock-related genes expressed in the ovaries of the Xiang pigs and found 33 clock-related genes differentially expressed between estrous and diestrous stages. We identified 44 differential splicing events from the transcripts of 34 biological clock-related genes. Furthermore, we also found 20 genes including the core clock components, arntl and cry1 were differentially regulated only at AS level and 14 genes, including per1 and clock, were differentially regulated at both expression and AS levels. We also proved that the core clock genes per1, cry1, clock and arntl and the clock-related genes, ppp1cb and ntrk1 were rhythmically expressed in Xiang pig ovaries by RT-qPCR experiments. The results demonstrated that the biological clock in the ovaries of Xiang pigs might play an important role in regulating the ovarian physiological functions by the transcriptional and post-transcriptional regulation.
- Published
- 2023
34. Albumin-based Theranostic Nanosystem for Drug Controlled Release and Magnetic Resonance Imaging Guided Therapy
- Author
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Qiaoying Wang, Jiali Cai, Zihan Xu, Shihui Huang, Ruiping Zhou, and Zhiyong Wang
- Subjects
Biophysics ,Bioengineering ,Biotechnology - Published
- 2021
35. Nondestructive Test Technology Research for Yarn Linear Density Unevenness
- Author
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Bin Zhou, Huiling Wang, Ke Wang, Jumei Zhao, Hongtao Zhou, and Shihui Huang
- Subjects
General Materials Science - Abstract
Yarn linear density and linear density unevenness between fragments involve the mass and mass variation of yarn per unit length, which are important indices to reflect the uniformity of yarn thickness. Aiming at the shortcomings of the traditional testing method, which uses a yarn length tester to test these indices by counting length first and then cutting and measuring weight, a testing device that measures weight and counts length synchronously is designed and developed, so that the yarn can be continuous, recyclable, and reusable. Length counting is conducted by a length-counting disk connected to a photoelectric coded disk, and the result of length counting can be accurate to 0.01 m. The original skein frame with a perimeter of 1 m is replaced, so that the error caused by yarn overlapping is avoided. Through program control, the testing of a plurality of groups of linear density values can be completed at one time to calculate the linear density unevenness of different fragments of yarn, and the yarn can be led to a recovery spool through a yarn guide cylinder to form a new package. Polyester/viscose 65/35 blended yarn was taken as a test sample and subjected to statistical analysis using SPSS software. It is found that the results of the newly developed device are closer to the arbitration value; the whole experiment is completed at one time, which avoids the secondary error and reduces the labor intensity; and the raw materials can be recycled, which saves labor and raw material costs. The device has high value for industrialization and popularization.
- Published
- 2021
36. Impact of fluoxetine preexposure on MDMA-induced taste avoidance in male and female rats
- Author
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Sara K. Bowman, Shihui Huang, Marissa E. Vasquez, Kenner C. Rice, and Anthony L. Riley
- Subjects
Pharmacology ,Psychiatry and Mental health ,Pharmacology (medical) - Abstract
The use of both prescription and illicit drugs creates the potential for drug interactions as a function of both pharmacokinetic and pharmacodynamic processes. One such interaction is that of fluoxetine and methylenedioxymethamphetamine (MDMA) in which fluoxetine attenuates the positive-like effects of MDMA. The present work extends the analysis of their interaction by examining the impact of fluoxetine on the aversive effects of MDMA which in balance with its rewarding effects may mediate its abuse potential. Male and female Sprague-Dawley rats were given fluoxetine (10 mg/kg every 4th day for five injections) prior to taste avoidance training with MDMA (3.2 mg/kg). MDMA induced taste avoidance in males and females (faster acquisition in females). Fluoxetine preexposure attenuated this avoidance in males, but not females. For males, the attenuation was partial as MDMA-conditioned animals with fluoxetine preexposure still displayed a significant reduction in fluid intake compared to controls. Consistent with prior work assessing the interaction of fluoxetine and MDMA, fluoxetine preexposure impacted the ability of MDMA to support taste avoidance learning, specifically attenuating the aversive effect of the drug. Prior work has shown that fluoxetine attenuates MDMA's positive effects which might lead to reduced intake of the drug; however, the concurrent reduction in the drug's aversive effects may still shift the overall affective balance of these two affective properties toward continued use and abuse. The fact that the attenuation was only evident in males needs further study to investigate the sex-dependent effects of drug history. (PsycInfo Database Record (c) 2022 APA, all rights reserved).
- Published
- 2022
37. A high-quality, phased genome assembly of broomcorn millet reveals the features of its subgenome evolution and 3D chromatin organization
- Author
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Zhiheng Wang, Shihui Huang, Zhengyue Yang, Jinsheng Lai, Xiang Gao, and Junpeng Shi
- Subjects
Cell Biology ,Plant Science ,Molecular Biology ,Biochemistry ,Biotechnology - Published
- 2023
38. Facile Synthesis of Iron Oxide Nanozymes for Synergistically Colorimetric and Magnetic Resonance Detection Strategy
- Author
-
Shihui, Huang, Shuqi, Jiang, Hong, Liu, Jiali, Cai, Gengjia, Chen, Junyao, Xu, Dan, Kai, Pengli, Bai, Ruiping, Zhou, and Zhiyong, Wang
- Subjects
Magnetic Resonance Spectroscopy ,Biomedical Engineering ,Pharmaceutical Science ,Medicine (miscellaneous) ,Colorimetry ,General Materials Science ,Bioengineering ,Ferric Compounds ,Magnetic Resonance Imaging - Abstract
Iron oxide nanomaterials with mimic enzymes activity have been paid more attention in the clinical diagnosis field. The modified surface molecules would influence the catalytic activity of nanozyme, which is worth studying. Furthermore, the traditional detection strategy is based on colorimetric change of substrates, however, the optical signal is easy to be interfered in complex biological applications. In our research, an efficient and facile preparation strategy was developed to obtain functional artificial nanozymes. Herein, three kinds of surfactants, including citrate acid, poly(ethylene glycol) bis (carboxymethyl) ether and tannic acid have been applied to modify these nanomaterials that showed uniform size, high soluble dispersity and stability. Furthermore, these nanozymes exhibited different peroxidase-like activity to catalyze the hydrogen peroxide and 3,3′,5,5′-tetramethylbenzidine. More importantly, magnetic relaxation effect of iron oxide nanozymes was found to be changed during the catalytic reaction. In addition, the relationship between the magnetic signal of nanozymes and the substrate concentration showed a good linear dependence. Combined with the natural enzymes, the magnetic detection of iron oxide nanozymes also exhibited excellent substrate specificity. On these bases, a dual-function specific assay was constructed and further used for glucose detection. In conclusion, this study demonstrated an efficient iron oxide nanozymes preparation method and constructed a new synergistically colorimetric-magnetic diagnosis strategy.
- Published
- 2021
39. Effect of Cooling Rate on the Grain Morphology and Element Segregation Behavior of Fe-Mn-Al-C Low-Density Steel during Solidification
- Author
-
Shihui Huang, Guangqiang Li, Zhan Zhang, Qingbiao Tan, and Guoliang Zhu
- Subjects
Fe-Mn-Al-C steel ,cooling rate ,microstructure ,elemental segregation ,Process Chemistry and Technology ,Chemical Engineering (miscellaneous) ,Bioengineering - Abstract
Mold with diameter sizes of 140 mm, 80 mm, 40 mm and 15 mm were designed to obtain the ingot of Fe-30Mn-10Al-1.1C low-density steel under different cooling rates. The influence of cooling rate on the grain morphology and elemental segregation behavior during the steel solidification process was analyzed by methods including inductively coupled plasma, scanning electron microscope and energy dispersive spectroscopy. The solidification sequence of the low-density steel was calculated by JMatPro 7.0 thermodynamic software. The results show that the microstructure of the steel is mainly austenite and contains a small amount of ferrite. The solidification order in the steel is: L → α, L → γ and α → γ, L → γ + MC. As the cooling rate increases from 1.69 °C/s to 10.28 °C/s, the ferrite phase precipitation increases by 16.7%, and the grain size decreases significantly, and in particular, the austenitic grain size decreases by 26%. With the increase in cooling rate, the microscopic segregation value of aluminum decreases approximately to 1. Additionally, the microscopic segregation of manganese showed a trend of increasing first and then decreasing. Microscopic segregation of Al and Mn can be improved significantly by increasing the cooling rate.
- Published
- 2022
- Full Text
- View/download PDF
40. Stridulatory sound production and acoustic signals of the longhorn beetle Batocera lineolata (Coleoptera: Cerambycidae)
- Author
-
Changqing Luo and Shihui Huang
- Subjects
Ecology ,media_common.quotation_subject ,Zoology ,Insect ,Sound production ,Biology ,Batocera lineolata ,Ecology, Evolution, Behavior and Systematics ,Longhorn beetle ,media_common - Abstract
Insects have evolved different structures and ways to produce sounds which play a crucial role in many aspects of insect biology, such as reproduction and predator–prey interactions. Among acoustic...
- Published
- 2021
41. Modeling and High-performance Trajectory Optimization of the Industrial Robot
- Author
-
Shihui Huang, Yamei Lin, Aipeng Jiang, and Guangsheng Qiu
- Subjects
History ,Computer Science Applications ,Education - Abstract
Rapidity, accuracy and energy cost are the key performance indexes for of the work of industrial robot. In this paper, the shortest time and the minimum energy consumption are taken as the goal for the comprehensive optimization. The control vector parameterization method is used to reasonably refine the time grid of the optimization problem. Then, an efficient control strategy for non-uniform adaptive mesh refinement is proposed. According to different changing trends, time nodes are merged or inserted into the time grid. While reducing the number of discrete grids, it also ensures the accuracy of the solution. Taking the Manutec R3 industrial robot as the object, the trajectory optimization simulation calculation is carried out. According to the verification of the simulation computing, the proposed method can significantly improve the time grid refinement. Compared with the traditional CVP method, not only the calculation time is shortened, but also more accurate results can be obtained.
- Published
- 2023
42. Methylone Pre-Exposure Differentially Impacts the Aversive Effects of MDPV and MDMA in Male and Female Sprague-Dawley Rats: Implications for Abuse Vulnerability
- Author
-
Hayley N. Manke, Katharine H. Nelson, Shihui Huang, Jacob M. Bailey, Sara K. Bowman, Robert A. Jones, Sydney E. Cerveny, Kenner C. Rice, and Anthony L. Riley
- Subjects
Pharmacology ,Male ,History ,Pyrrolidines ,Dose-Response Relationship, Drug ,Polymers and Plastics ,Substance-Related Disorders ,N-Methyl-3,4-methylenedioxyamphetamine ,Clinical Biochemistry ,Toxicology ,Biochemistry ,Industrial and Manufacturing Engineering ,Methamphetamine ,Rats ,Rats, Sprague-Dawley ,Behavioral Neuroscience ,Animals ,Central Nervous System Stimulants ,Female ,Benzodioxoles ,Business and International Management ,Biological Psychiatry - Abstract
Polydrug use is well documented in synthetic cathinone users, although the consequences of such use are not well characterized. In pre-clinical research, a pre-exposure to a drug has been reported to attenuate the aversive effects of other drugs which has implications for their abuse potential. The goal of the present study was to investigate the impact of pre-exposure to the synthetic cathinone methylone on the aversive effects of MDPV and MDMA.Male and female Sprague-Dawley rats were exposed to 10 mg/kg of methylone every 4th day (for a total of five injections) prior to taste avoidance training with 1.8 mg/kg of MDPV or 1 mg/kg of MDMA.MDPV and MDMA induced taste avoidance in males and females (all p's 0.05). In males, methylone pre-exposure attenuated the avoidance induced by MDPV and MDMA (all p's 0.05) with the attenuation greater with MDPV. In females, methylone pre-exposure attenuated avoidance induced by MDPV (all p's 0.05), but it had no effect on those induced by MDMA (all p's 0.05).The effects of exposure to methylone on taste avoidance induced by MDPV and MDMA were drug- (MDPVMDMA) and sex- (MDMA only in males) dependent. The attenuating effects of methylone pre-exposure on MDPV and MDMA were discussed in terms of their shared neurochemical action. These findings suggest that a history of methylone use may reduce the aversive effects of MDPV and MDMA which may have implications for polydrug use involving the synthetic cathinones.
- Published
- 2022
43. Whole-genome sequence analysis reveals selection signatures for important economic traits in Xiang pigs
- Author
-
Xiying Wang, Xueqin Ran, Xi Niu, Shihui Huang, Sheng Li, and Jiafu Wang
- Subjects
Multidisciplinary ,Genome ,Swine ,Animals ,Sequence Analysis, DNA ,Selection, Genetic ,Polymorphism, Single Nucleotide ,Disease Resistance - Abstract
Xiang pig (XP) is one of the best-known indigenous pig breeds in China, which is characterized by its small body size, strong disease resistance, high adaptability, favorite meat quality, small litter sizes, and early sexual maturity. However, the genomic evidence that links these unique traits of XP is still poorly understood. To identify the genomic signatures of selection in XP, we performed whole-genome resequencing on 25 unrelated individual XPs. We obtained 876.70 Gb of raw data from the genomic libraries. The LD analysis showed that the lowest level of linkage disequilibrium was observed in Xiang pig. Comparative genomic analysis between XPs and other breeds including Tibetan, Meishan, Duroc and Landrace revealed 3062, 1228, 907 and 1519 selected regions, respectively. The genes identified in selected regions of XPs were associated with growth and development processes (IGF1R, PROP1, TBX19, STAC3, RLF, SELENOM, MSTN), immunity and disease resistance (ZCCHC2, SERPINB2, ADGRE5, CYP7B1, STAT6, IL2, CD80, RHBDD3, PIK3IP1), environmental adaptation (NR2E1, SERPINB8, SERPINB10, SLC26A7, MYO1A, SDR9C7, UVSSA, EXPH5, VEGFC, PDE1A), reproduction (CCNB2, TRPM6, EYA3, CYP7B1, LIMK2, RSPO1, ADAM32, SPAG16), meat quality traits (DECR1, EWSR1), and early sexual maturity (TAC3). Through the absolute allele frequency difference (ΔAF) analysis, we explored two population-specific missense mutations occurred in NR6A1 and LTBP2 genes, which well explained that the vertebrae numbers of Xiang pigs were less than that of the European pig breeds. Our results indicated that Xiang pigs were less affected by artificial selection than the European and Meishan pig breeds. The selected candidate genes were mainly involved in growth and development, disease resistance, reproduction, meat quality, and early sexual maturity. This study provided a list of functional candidate genes, as well as a number of genetic variants, which would provide insight into the molecular basis for the unique traits of Xiang pig.
- Published
- 2021
44. Impact of the Aversive Effects of Drugs on Their Use and Abuse
- Author
-
Anthony L. Riley, Hayley N. Manke, and Shihui Huang
- Subjects
Neuropsychology and Physiological Psychology ,Neurology ,Reward ,Substance-Related Disorders ,Taste ,Humans ,Neurology (clinical) ,General Medicine - Abstract
Drug use and abuse are complex issues in that the basis of each may involve different determinants and consequences, and the transition from one to the other may be equally multifaceted. A recent model of the addiction cycle (as proposed by Koob and his colleagues) illustrates how drug-taking patterns transition from impulsive (acute use) to compulsive (chronic use) as a function of various neuroadaptations leading to the downregulation of DA systems, upregulation of stress systems, and the dysregulation of the prefrontal/orbitofrontal cortex. Although the nature of reinforcement in the initiation and mediation of these effects may differ (positive vs. negative), the role of reinforcement in drug intake (acute and chronic) is well characterized. However, drugs of abuse have other stimulus properties that may be important in their use and abuse. One such property is their aversive effects that limit drug intake instead of initiating and maintaining it. Evidence of such effects comes from both clinical and preclinical populations. In support of this position, the present review describes the aversive effects of drugs (assessed primarily in conditioned taste aversion learning), the fact that they occur concurrently with reward as assessed in combined taste aversion/place preference designs, the role of aversive effects in drug-taking (in balance with their rewarding effects), the dissociation of these affective properties in that they can be affected in different ways by the same manipulations, and the impact of various parametric, experiential, and subject factors on the aversive effects of drugs and the consequent impact of these factors on their use and abuse potential.
- Published
- 2021
45. Detection of genomic structure variants associated with wrinkled skin in Xiang pig by next generation sequencing
- Author
-
Xiaoli, Liu, primary, Fengbin, Hu, additional, Shihui, Huang, additional, Xi, Niu, additional, Sheng, Li, additional, Zhou, Wang, additional, Xueqin, Ran, additional, and Jiafu, Wang, additional
- Published
- 2021
- Full Text
- View/download PDF
46. Metagenomic profiles of the resistome in subtropical estuaries: Co-occurrence patterns, indicative genes, and driving factors
- Author
-
Lei Zhou, Peng Xu, Jiayi Gong, Shihui Huang, Wenjian Chen, Binwei Fu, Zelong Zhao, and Xiande Huang
- Subjects
Environmental Engineering ,Genes, Bacterial ,Environmental Chemistry ,Drug Resistance, Microbial ,Metagenomics ,Estuaries ,Pollution ,Waste Management and Disposal ,Ecosystem ,Anti-Bacterial Agents - Abstract
Estuaries are resistome hotspots owing to resistome accumulation and propagation at these locations from surrounding rivers, yet the large-scale biogeographic pattern of resistome, especially biocide and metal resistance genes (BMRGs) and its driving mechanisms in estuarine waters remains to be elucidated. Here, a metagenomics-based approach was firstly used to investigate resistome and mobilome profiles in waters from 30 subtropical estuaries, South China. The Pearl River estuaries had a higher diversity and abundance of antibiotic resistance genes (ARGs), BMRGs, and mobile genetic elements (MGEs) when compared with estuaries from east and west regions. Genes resistant to multiple antibiotics, metals, and biocides were the most abundant gene types in the resistome. The abundance of MGEs (e.g., intI1, IS91, and tnpA) was highly associated with the total abundance of resistance genes, suggesting their utility as potential indicators for quantitative estimations of the resistome contamination. Further, MGEs contributed more than bacterial communities in shaping the resistome in subtropical estuaries. Physicochemical factors (e.g., pH) regulated MGE composition and stochastic assembly, which mediated the co-selection of ARGs and BMRGs via horizontal gene transfer. Our findings have important implications and provide a reference on the management of ARGs and BMRGs in subtropical estuarine ecosystems.
- Published
- 2021
47. 15. Why Not Promote Nativist Literature?
- Author
-
Shihui, Huang, primary
- Published
- 2014
- Full Text
- View/download PDF
48. Ethanol pre-exposure differentially impacts the rewarding and aversive effects of α-pyrrolidinopentiophenone (α-PVP): Implications for drug use and abuse
- Author
-
Katharine H. Nelson, Shihui Huang, Kenner C. Rice, Jacob M. Bailey, Anthony L. Riley, Anna Vlachos, Tania D. Weiss, Karina J. Maradiaga, and Hayley N. Manke
- Subjects
Drug ,Male ,Taste ,Pyrrolidines ,Substance-Related Disorders ,media_common.quotation_subject ,Clinical Biochemistry ,Conditioning, Classical ,Self Administration ,Pharmacology ,Toxicology ,Biochemistry ,Article ,Body Temperature ,Rats, Sprague-Dawley ,Behavioral Neuroscience ,chemistry.chemical_compound ,Alkaloids ,Reward ,Pentanones ,Avoidance Learning ,Medicine ,Animals ,Reactivity (psychology) ,Biological Psychiatry ,media_common ,Ethanol ,business.industry ,Conditioned place preference ,Rats ,chemistry ,Body Temperature Changes ,Conditioning ,Central Nervous System Stimulants ,business ,Locomotion ,Bath salts - Abstract
Rationale Exposure to a drug can subsequently impact its own reactivity as well as that of other drugs. Given that users of synthetic cathinones, i.e., “bath salts”, typically have extensive and varied drug histories, an understanding of the effects of drug history on the behavioral and physiological consequences of synthetic cathiones may be important to their abuse liability. Objectives The goal of the current work was to assess the effects of an ethanol pre-exposure on the rewarding and aversive effects of α-PVP. Methods Adult male Sprague Dawley rats were exposed to ethanol prior to combined conditioned taste avoidance/conditioned place preference training in which rats were injected with 1.5, 3 or 5 mg/kg of racemic α-PVP or vehicle. Following a 7-day washout period, rats were then tested for thermoregulatory effects of α-PVP using subcutaneous probes to measure body temperature changes over the course of 8 h. This was followed 10 days later by assessments for α-PVP-induced locomotor activity and stereotypies over a 1-h session. Results α-PVP induced significant dose- and trial-dependent taste avoidance that was significantly attenuated by ethanol history and dose- and time-dependent increases in locomotor activity that were significantly increased by ethanol. α-PVP also induced place preferences and dose- and time-dependent increases in body temperature, but these measures were unaffected by ethanol history. Conclusions α-PVP's aversive effects (as measured by taste avoidance) were attenuated, while its rewarding effects (as indexed by place preference conditioning) were unaffected, by ethanol pre-exposure. Such a pattern may indicate increased α-PVP abuse liability, as changes in the balance of aversion and reward may impact overall drug effects and likelihood of drug intake. Future self-administration studies will be necessary to explore this possibility.
- Published
- 2021
49. A core–shell polymeric–inorganic hybrid nanocomposite system for MRI-visible gene delivery application in cancer immunotherapy
- Author
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Hua Ai, Changhui Deng, Rongrong Jin, Xuexia Yuan, Zhiyong Wang, Jing Zhao, Shihui Huang, Chen Guochuang, Gengjia Chen, and Jiali Cai
- Subjects
Nanocomposite ,Biocompatibility ,Chemistry ,General Chemical Engineering ,medicine.medical_treatment ,02 engineering and technology ,Immunotherapy ,Transfection ,Gene delivery ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Cell biology ,Gene product ,Immune system ,Cancer immunotherapy ,medicine ,0210 nano-technology - Abstract
Due to the tumor immune escape mechanism, strategies like the utilization of bispecific antibodies were used to assist immunotherapy. Herein a visible polymer–inorganic hybrid gene vehicle had been prepared to deliver anti-EpCAM/anti-CD3 encoding minicircle DNA into normal cells thus these transfected cells effectively secreted bispecific antibody with bioactivity. In the experiments, this compound system exhibited excellent biocompatibility, high transfection efficiency and ultrasensitive imaging capacity. Furthermore, the transfected cells could be detected under the MR imaging system and the gene product showed an outstanding immune effect. In conclusion, this nanocomposite showed synergistic advantages in gene delivery and non-invasive MR imaging.
- Published
- 2019
50. Spatio-temporal information for human action recognition.
- Author
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Li Yao, Yunjian Liu, and Shihui Huang
- Published
- 2016
- Full Text
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