5 results on '"Shigeki TAKEURA"'
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2. Analysis of Nuclear Accumulation of Influenza NP Antigen in von Magnus Virus-Infected Cells
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Yoshiyuki Nagai, Motohiro Shibata, Michinari Hamaguchi, Koichiro Maeno, Masao Iinuma, Shigeki Takeura, Toshisada Matsumoto, and Hiizu Aoki
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Peptide Biosynthesis ,Arginine ,viruses ,Immunology ,Biology ,Microbiology ,Virus ,chemistry.chemical_compound ,Species Specificity ,Antigen ,Virology ,Influenza, Human ,medicine ,Humans ,Antigens, Viral ,Ribonucleoprotein ,Cell Nucleus ,RNA ,Orthomyxoviridae ,Molecular biology ,Clone Cells ,Nucleoproteins ,medicine.anatomical_structure ,Ribonucleoproteins ,chemistry ,Cytoplasm ,Nucleus ,Canavanine - Abstract
When 1-50-4 cells were infected with von Magnus virus derived from influenza AJRIJ5+ virus by four successive undiluted passages in chick embryos, virus-specific proteins were synthesized but production of infectious virus was inhibited. In these cells the synthesis of viral RNA was suppressed and the nu cleoprotein (NP) antigen was found predominantly in the nucleus in contrast to standard virus-infected cells in which the antigen was distributed throughout the whole cell. The intracellular location and migration of NP were determined by isotope labeling and sucrose gradient centrifugation of subcellular fractions. In standard virus-infected cells NP polypeptide was present predominantly in the cytoplasm in the form of viral ribonucleoprotein (RNP) and intranuclear RNP was detected in reduced amounts. In contrast, in von Magnus virus-infected cells NP polypeptide was present predominantly in the nucleus in a non assembled, soluble form and the amount of cytoplasmic RNP was considerably reduced. After short-pulse labeling NP was detected exclusively in the cytoplasm in a soluble form and after a chase a large proportion of such soluble NP was seen in the nucleus. It is suggested that a large proportion of the NP synthesized in von Magnus virus-infected cells is not assembled into cytoplasmic RNP because of the lack of available RNA and the NP migrated into the nucleus and remained there. Canavanine, an arginine analog, is a potent inhibitor of the growth of influenza virus (11). It inhibits the synthesis of viral RNA and the formation of viral RNP. Under these conditions, most of the NP is found in the nucleus in a nonassembled, soluble form, in contrast to untreated cells in which a large amount of NP is distri buted in the cytoplasm in the form of RNP. Influenza RNP consists of multiple molecules ofNP and one segment of virion RNA (vRNA) or cRNA complementary to vRNA (2, 17). We have suggested the possibility that the NP synthesized in the presence of canavanine is not assembled into cytoplasmic RNP because of the lack of available RNA and that the NP readily migrates into the nucleus and ac
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- 1981
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3. Analysis of the Inhibitory Effect of Canavanine on the Replication of Influenza RI/5+Virus: II. Interaction of M Protein with the Plasma Membrane
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Tatsuya Tsurumi, Motohiro Shibata, Michinari Hamaguchi, Shigeki Takeura, Koichiro Maeno, Yasuo Sugiura, Hiizu Aoki, and Yoshiyuki Nagai
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Arginine ,Myeloma protein ,Immunology ,Fluorescent Antibody Technique ,Hemagglutinin (influenza) ,Chick Embryo ,Virus Replication ,Microbiology ,Virus ,Cell Line ,Canavanine ,Viral Proteins ,chemistry.chemical_compound ,Virology ,Extracellular ,Animals ,Hemadsorption ,biology ,Vesicle ,Cell Membrane ,Clone Cells ,Nucleoprotein ,Microscopy, Electron ,chemistry ,Biochemistry ,Influenza A virus ,biology.protein - Abstract
When influenza A/RI/5+ virus-infected cells were incubated in medium to which 2 micrograms of canavanine (arginine analog) per ml had been added 4 hr after infection, all viral polypeptides were synthesized but the budding-like process with the appearance of extracellular virus was completely inhibited. The plasma membrane isolated from these cells contained exclusively hemagglutinin (HA), and membrane (M) protein and nucleoprotein (NP) appeared to be associated with the nucleus, in contrast to untreated cells whose plasma membrane contained abundant HA, M protein, and NP. Disruption of canavanine-treated cells by freeze-thawing generated a number of hemagglutinating membranous vesicles or fragments containing exclusively HA. By isotope labeling it was found that the M protein synthesized in the presence of canavanine, together with HA and NP, is a canavanine-substituted polypeptide. It is suggested that canavanine inhibits the formation of the mature envelope of influenza RI/5+, because of the inability of M protein to associate with the plasma membrane.
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- 1981
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4. Comparative Study of BRL28500 (Clavulanic Acid-Ticarcilin) and Piperacillin in the Treatment of Respiratory Tract Infections
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Fumio MIKI, Yoshiyasu IKUNO, Eiji INOUE, Akihito MURATA, Shinichi TANIZAWA, Kazuo SAKAMOTO, Akira TAHARA, Akira SAITO, Masumi TAMIZAWA, Yohmei HIRAGA, Kohki KIKUCHI, Tomoko YAMAMOTO, Kazuo TAKABE, Teruo NAKAMURA, Tadashi MIYAZAWA, Toyokazu TAMURA, Katsumi ENDO, Masashi YONEDA, Yasuo IDO, Osamu UEHARA, Katsuhiro OKAMOTO, Morio SAGARA, Tamotsu TAKISHIMA, Shiro IDA, Kiyoshi KONNO, Kotaro OIZUMI, Seiichi AONUMA, Akira WATANABE, Kazuo SATO, Izumi HAYASHI, Masataka KATSU, Shinji OKUI, Mieko KAWAI, Toshio FUKUI, Masaaki ARAKAWA, Koichi WADA, Takao MORIMOTO, Tomoko KABASAWA, Hajimu TAKEDA, Osamu SEKINE, Yoshimaru USUDA, Nobuki AOKI, Tadashi MIYAHARA, Atsushi SAITO, Jingoro SHIMADA, Kohya SHIBA, Hideo IKEMOTO, Kazuyoshi WATANABE, Hiroyuki KOBAYASHI, Kenji TAKAMURA, Masahiko YOSHIDA, Keimei MASHIMO, Yoshiji YAMANE, Toshiharu TOMI, Junzaburo KABE, Hiroyoshi ISHIBASHI, Koichiro KUDO, Takeshi OTA, Hiroichi TANIMOTO, Ryuo NAKATANI, Kunihiko YOSHIMURA, Yoshitaka NAKAMORI, Naohiko CHONABAYASHI, Koichiro NAKATA, Kentaro WATANABE, Masaru KOYAMA, Fukuo IIJIMA, Takashi INAMATSU, Kyoko URAYAMA, Fuyuhiko AZUMA, Yuzo FUNATSU, Ippei FUJIMORI, Yoshio KOBAYASHI, Masanori ADACHI, Kazufuto FUKAYA, Takao OKUBO, Akira ITO, Hiroshi MATSUMOTO, Junichi SUZUKI, Yasuhiro YOSHIIKE, Yuji WATANUKI, Shigeki ODAGIRI, Jun CHIBA, Kaneo SUZUKI, Koou MUROHASHI, Tsutomu FUKUDA, Mitsuyo KIUCHI, Yasuhiko ASHIKARI, Kaoru SHIMOKATA, Saiji YOSHII, Osamu TAKANOU, Shuzo SAKAI, Keisuke NISHIWAKI, Shigeki TAKEURA, Hirotsugu KISHIMOTO, Tatsuo SATAKE, Kenzo TAKAGI, Kenichi YAMAKI, Motohide SASAMOTO, Tomoyasu SASAKI, Toshihiko TAKEUCHI, Masahito KATO, Joichi KATO, Goh ITO, Toshiyuki YAMAMOTO, Kanzo SUZUKI, Kazuhide YAMAMOTO, Satoru ADACHI, Kaoru OYAMA, Kunikazu SUZUKI, Nobuo OTANI, Mitsuru HAYASE, Fumiyuki KUZE, Hiroyuki TSUJINO, Nobuo INABA, Nobuaki IKEDA, Tsuneo MATSUBARA, Shinichi USHIDA, Ryoichi AMITANI, Michiyasu NAKANISHI, Hiroshi OKUBO, Yoshihiro UEDA, Nobuhiro NARITA, Masayoshi SAWAKI, Keiichi MIKASA, Kojiro YASUNAGA, Seibun YONETSU, Yube IIDA, Yoshihiko SAKAKIBARA, Eiroh TSUBURA, Tomoo HAMADA, Koichi FUKUYAMA, Masahiro FUKUOKA, Masami ITO, Fumio HIRAO, Takashi KOMATSU, Nobuo MAEKAWA, Hideki NISHIYAMA, Yujiro SUZUKI, Sadao HORIKAWA, Masakazu TAMURA, Rinzo SOEJIMA, Yoshihito NIKI, Michifumi ADACHI, Yoshihisa NAKAGAWA, Masaru SUMI, Osamu KURIMURA, Hideyo SASAKI, Hirofumi FUKUHARA, Tadao MORIMOTO, Yoshiro SAWAE, Kaoru OKADA, Yukio KUMAGAI, Nobuaki SHIGEMATSU, Hisamichi AIZAWA, Masahide TAKII, Takafumi OHDO, Tomoaki SHINAGAWA, Kohei HARA, Masaki HIROTA, Keizo YAMAGUCHI, Shigeru KOHNO, Hironobu KAGA, Koichi WATANABE, Kiyo FUJITA, Yasuko UEDA, Kota KOHNO, Keizo MATSUMOTO, Tsuyoshi NAGATAKE, Naoto RIKITOMI, Masaru NASU, Jun GOTO, Yoichiro GOTO, Hideaki SHIGENO, and Takayoshi TASHIRO
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Adult ,Male ,Penicillins ,Microbiology ,Clavulanic Acids ,Clavulanic acid ,medicine ,Humans ,Ticarcillin ,Respiratory Tract Infections ,Beta-Lactamase Inhibitors ,Aged ,Aged, 80 and over ,Piperacillin ,Clinical Trials as Topic ,Respiratory tract infections ,Chemistry ,General Medicine ,Middle Aged ,Clavulanic acid + Ticarcillin ,Drug Combinations ,Female ,beta-Lactamase Inhibitors ,medicine.drug - Published
- 1987
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5. [Comparative study of BRL28500 (clavulanic acid-ticarcillin) and ticarcillin in the treatment of respiratory tract infections]
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Fumio MIKI, Yoshiyasu IKUNO, Eiji INOUE, Akihito MURATA, Shinichi TANIZAWA, Kazuo SAKAMOTO, Akira TAHARA, Akira SAITO, Masumi TOMIZAWA, Yohmei HIRAGA, Kohki KIKUCHI, Tomoko YAMAMOTO, Kazuo TAKEBE, Teruo NAKAMURA, Tadashi MIYAZAWA, Toyokazu TAMURA, Katsumi ENDO, Masashi YONEDA, Yasuo IDO, Osamu UEHARA, Katsuhiro OKAMOTO, Morio SAGARA, Tamotsu TAKISHIMA, Shiro IDA, Kiyoshi KONNO, Kotaro OIZUMI, Seiichi AONUMA, Akira WATANABE, Kazuo SATO, Izumi HAYASHI, Masataka KATSU, Shinji OKUI, Mieko KAWAI, Toshio FUKUI, Masaaki ARAKAWA, Koichi WADA, Takao MORIMOTO, Tomoko KABASAWA, Hajimu TAKEDA, Osamu SEKINE, Yoshimaru USUDA, Nobuki AOKI, Tadashi MIYAHARA, Atsushi SAITO, Jingoro SHIMADA, Kohya SHIBA, Hideo IKEMOTO, Kazuyoshi WATANABE, Hiroyuki KOBAYASHI, Kenji TAKAMURA, Masahiko YOSHIDA, Keimei MASHIMO, Yoshiji YAMANE, Toshiharu TOMI, Junzaburo KABE, Hiroyoshi ISHIBASHI, Koichiro KUDO, Takeshi OTA, Hiroichi TANIMOTO, Ryuo NAKATANI, Kunihiko YOSHIMURA, Yoshitaka NAKAMORI, Naohiko CHONABAYASHI, Koichiro NAKATA, Kentaro WATANABE, Masaru KOYAMA, Fukuo IIJIMA, Takashi INAMATSU, Kyoko URAYAMA, Fuyuhiko AZUMA, Yuzo FUNATSU, Ippei FUJIMORI, Yoshio KOBAYASHI, Masanori ADACHI, Kazufuto FUKAYA, Takao OKUBO, Akira ITO, Hiroshi MATSUMOTO, Junichi SUZUKI, Yasuhiro YOSHIIKE, Yuji WATANUKI, Shigeki ODAGIRI, Jun CHIBA, Kaneo SUZUKI, Koou MUROHASHI, Tsutomu FUKUDA, Mitsuyo KIUCHI, Yasuhiko ASHIKARI, Kaoru SHIMOKATA, Saiji YOSHII, Osamu TAKANOU, Shuzo SAKAI, Keisuke NISHIWAKI, Shigeki TAKEURA, Hirotsugu KISHIMOTO, Tatsuo SATAKE, Kenzo TAKAGI, Kenichi YAMAKI, Motohide SASAMOTO, Tomoyasu SASAKI, Toshihiko TAKEUCHI, Masahito KATO, Joichi KATO, Goh ITO, Toshiyuki YAMAMOTO, Kanzo SUZUKI, Kazuhide YAMAMOTO, Satoru ADACHI, Kaoru OYAMA, Kunikazu SUZUKI, Nobuo OTANI, Mitsuru HAYASE, Fumiyuki KUZE, Hiroyuki TSUJINO, Nobuo INABA, Nobuaki IKEDA, Tsuneo MATSUBARA, Shinichi USHIDA, Ryoichi AMITANI, Michiyasu NAKANISHI, Hiroshi OKUBO, Yoshihiro UEDA, Nobuhiro NARITA, Masayoshi SAWAKI, Keiichi MIKASA, Kojiro YASUNAGA, Seibun YONETSU, Yube IIDA, Yoshihiko SAKAKIBARA, Eiroh TSUBURA, Tomoo HAMADA, Koichi FUKUYAMA, Masahiro FUKUOKA, Masami ITO, Fumio HIRAO, Takashi KOMATSU, Nobuo MAEKAWA, Hideki NISHIYAMA, Yujiro SUZUKI, Sadao HORIKAWA, Masakazu TAMURA, Rinzo SOEJIMA, Yoshihito NIKI, Michifumi ADACHI, Yoshihisa NAKAGAWA, Masaru SUMI, Osamu KURIMURA, Hideyo SASAKI, Hirofumi FUKUHARA, Tadao MORIMOTO, Yoshiro SAWAE, Kaoru OKADA, Yukio KUMAGAI, Nobuaki SHIGEMATSU, Hisamichi AIZAWA, Masahide TAKII, Takafumi OHDO, Tomoaki SHINAGAWA, Kohei HARA, Masaki HIROTA, Keizo YAMAGUCHI, Shigeru KOHNO, Hironobu KOGA, Koichi WATANABE, Kiyo FUJITA, Yasuko UEDA, Kota KOHNO, Keizo MATSUMOTO, Tsuyoshi NAGATAKE, Naoto RIKITOMI, Masaru NASU, Jun GOTO, Yoichiro GOTO, Hideaki SHIGENO, and Takayoshi TASHIRO
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Adult ,Aged, 80 and over ,Clinical Trials as Topic ,Chemistry ,General Medicine ,Penicillins ,Middle Aged ,Clavulanic acid + Ticarcillin ,Microbiology ,Clavulanic Acids ,Drug Combinations ,Ticarcillin ,medicine ,Humans ,beta-Lactamase Inhibitors ,Respiratory Tract Infections ,medicine.drug ,Aged - Published
- 1987
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