1. Aspirin differentially regulates endotoxin-induced IL-12 and TNF-alpha production in human dendritic cells.
- Author
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Ho, Ling-Jun, Chang, Deh-Ming, Shiau, Hen-Yih, Chen, Chen-Hung, Hsieh, Tsai-Yuan, Hsu, Yu-Lin, Wong, Chih-Shung, Lai, Jenn-Haung, Ho, L J, Chang, D M, Shiau, H Y, Chen, C H, Hsieh, T Y, Hsu, Y L, Wong, C S, and Lai, J H
- Subjects
ASPIRIN ,DENDRITIC cells ,PHYSIOLOGY ,BIOCHEMISTRY ,CELL culture ,COMPARATIVE studies ,DOCUMENTATION ,DOSE-effect relationship in pharmacology ,INTERLEUKINS ,PHENOMENOLOGY ,RESEARCH methodology ,MEDICAL cooperation ,NUCLEOTIDES ,POLYMERASE chain reaction ,REFERENCE values ,RESEARCH ,TUMOR necrosis factors ,EVALUATION research ,REVERSE transcriptase polymerase chain reaction ,LIPOPOLYSACCHARIDES ,PHARMACODYNAMICS - Abstract
Objective: In the development of autoimmune diseases, dendritic cells (DC) play critical roles. Here, we examined the effect of aspirin on lipopolysaccharide (LPS)-induced DC activation.Methods: The monocyte-derived DC were established. The cytokine production was measured by ELISA, reverse transcriptase/polymerase chain reaction, or intracellular staining analyzed by flow cytometry. The expression of cell surface molecules was determined by flow cytometry.Results: Aspirin inhibited LPS-induced DC maturation and costimulatory molecules expression. Aspirin, at therapeutic concentrations, also decreased LPS-induced IL-12 and IL-10 production. In contrast, the LPS-induced TNF-alpha production was enhanced by aspirin. The differential effects of aspirin on IL-12 and TNF-alpha production may not be due to down-regulation of cyclooxygenase activities.Conclusion: The various effects of aspirin on LPS-stimulated DC may influence the understanding of the diverse immunomodulatory mechanisms of this anti-inflammatory drug. [ABSTRACT FROM AUTHOR]- Published
- 2001
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