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2 results on '"Shi En Koh"'

1. Engineered NS1 for Sensitive, Specific Zika Virus Diagnosis from Patient Serology

Author

Thai Leong Yap, Shin Yee Hong, Jun Hui Soh, Lekha Ravichandraprabhu, Vanessa W.X. Lim, Hsi-Min Chan, Tommy Z.X. Ong, Ying Ping Chua, Shi En Koh, Huajing Wang, Yee Sin Leo, Jackie Y. Ying, and William Sun

Subjects
Zika, dengue, flavivirus, viruses, monoclonal antibody, serologic test, Medicine, Infectious and parasitic diseases, RC109-216
Abstract

Dengue virus (DENV) and Zika virus (ZIKV) belong to the Flaviviridae family of viruses spread by Aedes aegypti mosquitoes in tropical and subtropical areas. Accurate diagnostic tests to differentiate the 2 infections are necessary for patient management and disease control. Using characterized ZIKV and DENV patient plasma in a blind manner, we validated an ELISA and a rapid immunochromatographic test for ZIKV detection. We engineered the ZIKV nonstructural protein 1 (NS1) for sensitive serologic detection with low cross reactivity against dengue and developed monoclonal antibodies specific for the ZIKV NS1 antigen. As expected, the serologic assays performed better with convalescent than acute plasma samples; the sensitivity ranged from 71% to 88%, depending on the performance of individual tests (IgM/IgG/NS1). Although serologic tests were generally less sensitive with acute samples, our ZIKV NS1 antibodies were able to complement the serologic tests to achieve greater sensitivity for detecting early infections.

Published
2021
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2. Engineered NS1 for Sensitive, Specific Zika Virus Diagnosis from Patient Serology

Author

Lekha Ravichandraprabhu, Shi En Koh, Huajing Wang, Yee Sin Leo, William Sun, Hsi-Min Chan, Ying Ping Chua, Shin Yee Hong, Jackie Y. Ying, Tommy Z.X. Ong, Vanessa Weixun Lim, Jun Hui Soh, and Thai Leong Yap

Subjects
Epidemiology, viruses, Infectious and parasitic diseases, RC109-216, Dengue virus, Viral Nonstructural Proteins, medicine.disease_cause, Antibodies, Viral, Serology, Dengue fever, Zika virus, 0302 clinical medicine, flavivirus, diagnostics, 030212 general & internal medicine, microcephaly, Engineered NS1 from Patient Serology for Sensitive, Specific Zika Virus Diagnosis, biology, Zika Virus Infection, virus diseases, serologic test, Flavivirus, Infectious Diseases, Medicine, Antibody, Microbiology (medical), 030231 tropical medicine, Enzyme-Linked Immunosorbent Assay, Aedes aegypti, Sensitivity and Specificity, 03 medical and health sciences, Flaviviridae, Zika, immunochromatography, medicine, Humans, Serologic Tests, business.industry, Research, Zika Virus, biochemical phenomena, metabolism, and nutrition, Dengue Virus, biology.organism_classification, medicine.disease, Virology, dengue, monoclonal antibody, biology.protein, business, immunoglobulin
Abstract

Dengue virus (DENV) and Zika virus (ZIKV) belong to the Flaviviridae family of viruses spread by Aedes aegypti mosquitoes in tropical and subtropical areas. Accurate diagnostic tests to differentiate the 2 infections are necessary for patient management and disease control. Using characterized ZIKV and DENV patient plasma in a blind manner, we validated an ELISA and a rapid immunochromatographic test for ZIKV detection. We engineered the ZIKV nonstructural protein 1 (NS1) for sensitive serologic detection with low cross reactivity against dengue and developed monoclonal antibodies specific for the ZIKV NS1 antigen. As expected, the serologic assays performed better with convalescent than acute plasma samples; the sensitivity ranged from 71% to 88%, depending on the performance of individual tests (IgM/IgG/NS1). Although serologic tests were generally less sensitive with acute samples, our ZIKV NS1 antibodies were able to complement the serologic tests to achieve greater sensitivity for detecting early infections.

Published
2021

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