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171 results on '"Shi, Xingbo"'

7. Colorimetric and Fluorescent Dual-Mode Detection of Aflatoxin B1 Using Composite Nanomaterial

Author

LI Yang, LU Dai, ZHOU Zhou, CHEN Liye, XIE Xinhui, WU Jiahao, ZHAO Qian, SHI Xingbo

Subjects
aflatoxin b1, manganese dioxide nanoparticles, graphene quantum dots, aptamer, dual-mode signal, Food processing and manufacture, TP368-456
Abstract

In this study, a highly sensitive portable platform with colorimetric and fluorescent dual-mode signal output for the detection of aflatoxin B1 (AFB1) was developed based on the reaction between glutathione and a composite nanomaterial consisting of manganese dioxide (MnO2) nanoparticles and graphene quantum dots (GQDs) as a substrate. The recognition probe was prepared by using silica nanoparticles as a carrier to enrich glutathione and AFB1 aptamers. Under optimal conditions, analytical figures of merit such as specificity of this method were studied. The regression equation for colorimetric signal was ΔA = − 0.275 − 0.021lgC and the detection limit was 2.732 × 10-12 g/mL; the regression equation for fluorescence signal was ΔF = 928.733 + 71.779lgC and the detection limit was 1.667 × 10-12 g/mL. Both methods had good detection specificity. The proposed method was applied in the detection of food samples, such as milk, rice, oatmeal, soy sauce and white vinegar with higher accuracy, compared with the traditional method.

Published
2023
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12. Amine-Responsive Anthocyanin-Loaded Hydrogel for Monitoring the Freshness of Fish and Shrimp

Author

DONG Shengye, ZHANG Yaqin, FU Zhaodi, SHI Xingbo

Subjects
purple sweet potato anthocyanins, hydrogel, aquatic products, freshness, red, green and blue method, Food processing and manufacture, TP368-456
Abstract

A visual, non-destructive method for monitoring fish and shrimp freshness was established using an amine-responsive agarose hydrogel loaded with purple sweet potato anthocyanin (PSPA). Trimethylamine (TMA) was selected as an indicator of fish and shrimp spoilage. The redshift of the absorption peak of PSPA hydrogels after reaction with different concentrations of TMA (0–194.22 × 10-9 mol/L) was investigated. The red (R), green (G), and blue (B) channel values of the hydrogels were analyzed using smartphone imaging combined with the Image J software. The concentration of TMA was quantified using G/R as the output signal. The limit of detection (LOD) was 0.84 × 10-9 mol/L and the linear range was 8.78 × 10-9–19.386 × 10-9 mol/L (R2 = 0.992 1). When used to detect the freshness of fish and shrimp, the proposed method showed a good correlation with the national standard method for measuring total volatile basic nitrogen (TVB-N) content with a Pearson correlation coefficient of 0.998 4 and 0.996 6, respectively. Due to the application of functional hydrogel and the introduction of smartphones, the method had the advantages of simplicity, non-destructiveness, and portability. This study provides a new method for on-site monitoring the real-time freshness of aquatic products, which has a broad application prospect in the field of aquatic food safety detection.

Published
2023
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