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20 results on '"Shi, Shuiqin"'

3. Association of ADRB2 gene polymorphisms and intestinal microbiota in Chinese Han adolescents

Author

Xu Shanrong, Liu Wenqi, Gong Li, Li Xinyang, Chu Wenwen, Han Meng, Shi Shuiqin, and Zhou Duoqi

Subjects
chinese han adolescents, adrb2 gene polymorphisms, intestinal microbiota, correlation analysis, Biology (General), QH301-705.5
Abstract

Gut microbiota are closely related to health, and the β2-adrenergic receptor gene (ADRB2) is associated with gastrointestinal diseases. In the present study, we aimed to explore the relationship between ADRB2 gene polymorphisms and gut microbiota in Chinese Han adolescents. The relative abundance, diversity, and PICRUSt function predictions of male and female gut microbiomes differ significantly and that ADRB2 gene polymorphisms were associated with gut microbiome abundance in Chinese Han adolescents.

Published
2023
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13. The novel probiotic preparation based on Lactobacillus spp. mixture on the intestinal bacterial community structure of Cherry Valley Duck

Author

Zhao, Kai, primary, Zhou, Duoqi, additional, Liu, Wenqi, additional, Zhang, Yixun, additional, Gai, Mengrui, additional, Han, Yu, additional, Li, Wenhui, additional, He, Guangyu, additional, Xiong, Yan, additional, Dong, Jinsheng, additional, Hu, Jie, additional, Liu, Yannan, additional, and Shi, Shuiqin, additional

Published
2022
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17. Lactobacillus fermentum 1.2133 display probiotic potential in vitro and protect against Salmonella pullorum in chicken of infection.

Author

Wang, Min, Hu, Jie, Yu, Han, Li, Wenhui, He, Guangyu, Dong, Jinsheng, Liu, Yannan, and Shi, Shuiqin

Subjects
LACTOBACILLUS fermentum, SALMONELLA, SALMONELLA diseases, AEROBIC bacteria, SMALL intestine, PROBIOTICS
Abstract

The efficacy of Lactobacillus as an antibiotic substitute has been investigated as one of the potential strategies to prevent Salmonella infection in poultry. The purpose of this study was to explore the antibacterial activity of Lactobacillus fermentum 1.2133 (Lact. fermentum 1.2133) against Salmonella pullorum CVCC533 (Salm. pullorum CVCC533) and its effect on chickens infected with Salm. pullorum CVCC533. Results showed that Lact. fermentans 1.2133 has antibacterial activity against Salm. pullorum CVCC533 and the cell-free fermentation supernatant of Lact. fermentum 1.2133 had a bactericidal effect on the bacteria in the Salm. pullorum CVCC533 biofilm by significantly reducing the number of Salmonella and aerobic bacteria in the chicken duodenum, ileum, and cecum, including Escherichia shigella (P  < 0.05), improved the species abundance of Lactobacilli (P  < 0.05). The damage to the chicken intestine by Salm. pullorum CVCC533 was reduced as the expression of avian beta-defensin 2 (AvBD2) mRNA in chicken small intestine was increased (P  < 0.05). The results showed that Lact. fermentum 1.2133 had the potential to be a probiotic for poultry due to its regulation of intestinal AvBD2 mRNA as well as its intestinal flora. [ABSTRACT FROM AUTHOR]

Published
2023
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20. Antagonistic activity and mechanism of Lactobacillus rhamnosusSQ511 against Salmonella enteritidis

Author

Shi, Shuiqin, Gong, Li, Yu, Hao, He, Guangyu, Zhang, Jingjing, Han, Yu, Liu, Yannan, Hu, Jie, Dong, Jinsheng, Liu, Jia, Zhao, Kai, and Zhou, Duoqi

Abstract

Salmonella enteritidisis an important food-borne pathogen. The use of antibiotics is a serious threat to animal and human health, owing to the existence of resistant strains and drug residues. Lactic acid bacteria, as a new alternative to antibiotics, has attracted much attention. In this study, we investigated the antibacterial potential and underlying mechanism of Lactobacillus rhamnosusSQ511 against S. enteritidisATCC13076. The results revealed that L. rhamnosusSQ511 significantly inhibited S. enteritidisATCC13076 growth or even caused death. Laser confocal microscopic imaging revealed that the cell-free supernatant (CFS) of L. rhamnosusSQ511 elevated the reactive oxygen species level and bacterial membrane depolarization in S. enteritidisATCC13076, leading to cell death. Furthermore, L. rhamnosusSQ511 CFS had severely deleterious effects on S. enteritidisATCC13076, causing membrane destruction and the release of cellular materials. In addition, L. rhamnosusSQ511 CFS significantly reduced the expression of virulence, motility, adhesion, and invasion genes in S. enteritidisATCC13076 (P< 0.05), and considerably inhibited motility and biofilm formation capacity (P< 0.05). Thus, antimicrobial compounds produced by L. rhamnosusSQ511 strongly inhibited S. enteritidisgrowth, mobility, biofilm formation, membrane disruption, and reactive oxygen species generation, and regulated virulence-related gene expressions, presenting promising applications as a probiotic agent.

Published
2022
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