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Cerebral ischemia activates neural progenitors that participate in brain remodeling following acute injury. Sphingosine‐1‐phosphate receptor (S1PR) signaling governs cell proliferation and mobilization, yet its potential impact on neural progenitors and stroke recovery remains poorly understood. The goal of this study was to investigate the impact of S1PR modulation on post‐stroke neurogenesis and functional recovery, using a S1PR modulator BAF312. Mice were subjected to 60 min middle cerebral artery occlusion (MCAO) and received BAF312 starting from day 3 after MCAO until the end of experiment. BAF312 facilitated motor function recovery in MCAO mice until day 14 after surgery. Flow cytometry analysis revealed that BAF312 treatment led to an increase of type A cells in subventricular zone (SVZ), but not other progenitor cell subsets in MCAO mice. We found an increase of BrdU incorporation in SVZ DCX+ cells from MCAO mice receiving BAF312 and augmented proliferation of DCX+ cells in cultured neurospheres isolated from SVZ tissues. Notably, a S1PR1 antagonist W146 abolished BAF312‐induced increase of SVZ type A cells from MCAO mice and proliferation of DCX+ cells in cultured neurospheres. Additionally, the benefit of BAF312 to improve neurogenesis and stroke recovery remains in Rag2−/− mice lacking of T and B cells. Our results demonstrate that S1PR modulation improves neurogenesis and functional recovery following brain ischemia. [ABSTRACT FROM AUTHOR]

In this paper, an optimal consensus-based event-triggered control strategy is proposed to coordinate multiple distributed energy resources (DERs) in a dc microgrid. In the proposed control strategy, the average bus voltage restoration and the proportional current sharing are integrated by transforming the coordinated control problem into an optimization problem. The proposed solution enhances the resilience of the controller strategy in extreme events with uncertain initial states. Accordingly, the proposed control strategy provides a sampled-data-based event-triggered mechanism considering the limited availability of communication resources, which reduces communication burdens and avoids Zeno behaviors. The sufficient conditions are demonstrated using the Lyapunov–Krasovskii functional method for maintaining the stability of the proposed control strategy. Several case studies are presented using the PSCAD/EMTDC platform and the results are discussed to validate the effectiveness of the proposed control strategy for enhancing the dc microgrid resilience. [ABSTRACT FROM AUTHOR]

The conventional voltage observer based distributed control can make a tradeoff between voltage restoration and reactive power sharing. However, it suffers from estimation error when there exist input disturbances, such as initial values and communication delays. To address this problem, this paper proposes a novel distributed secondary control based on PI consensus algorithm. A voltage controller and a frequency controller are designed separately to realize the restoration of voltage/frequency and also accurate reactive/active power allocation. Moreover, a detailed small-signal model with PI consensus algorithm is established to show the impact of the proposed control strategy on the system stability. Compared with the conventional distributed control, the proposed control scheme is simpler with higher control accuracy under input disturbances. Simulations are conducted to verify the effectiveness of the proposed control strategy in an isolated ac microgrid. [ABSTRACT FROM AUTHOR]

Hybrid energy storage system (HESS) consisting of battery and supercapacitor (SC) is an effective approach to alleviate voltage stability problems brought by the fluctuation of renewable resources in a dc microgrid. This paper proposes a novel distributed control scheme for multiple HESSs based on a leaderless consensus protocol to realize the power splitting between batteries and SCs and thus regulate the dc bus voltage. Without assigning leaders among the batteries and SCs, each SC or battery has equal priority in participating in voltage or state of charge (SOC) regulation using only neighboring information exchange instead of global communication. This distributed approach makes the system robust against any physical failure or communication fault only if the remaining communication network is connected. Consensus for the state variable associated with the battery SOC, the SC terminal voltage, and the dc average bus voltage are all achieved in this scheme, and thus the voltage quality and the energy storage lifetime can be improved. In addition, the small-signal stability of a four-HESS system is investigated, and the impact of the control parameters on the system is analyzed. Simulations and experimental studies are conducted to demonstrate the effectiveness of the proposed control scheme in an islanded dc microgrid. [ABSTRACT FROM AUTHOR]

In this paper, a distributed cooperative control scheme, considering the state-of-charge balance and power limits of battery-storage units (BSUs) in the consensus protocol, is proposed to achieve the average bus voltage consensus in a dc microgrid with photovoltaics. The state variable defined in the consensus protocol enables all BSUs to charge or discharge together without introducing circulating currents among BSUs. Furthermore, by incorporating power limits of BSUs in the defined state variable, power violation of the BSUs can be prevented. The consensus-based distributed control only utilizes neighbor-to-neighbor communication to realize the global consensus, and, thus, avoids the single point of the failure compared to the conventional centralized control. Simulation and experimental results demonstrate the efficacy of the proposed method in an islanded dc microgrid under different testing scenarios. [ABSTRACT FROM AUTHOR]

Biochar amendment in agroecosystems is proposed to mitigate the greenhouse (GHG) emissions and enhance multiple ecosystem services. However, it remains unclear whether and how various biochar amendment rates can influence soil microbial communities linking with GHG mitigation. In a two-year field experiment with five biochar amendment rates (0, 10, 20, 30 and 40 Mg ha−1), we determined cumulative GHG emissions, soil physiochemical properties and soil microbial community composition base one total phospholipid fatty acids (PLFA) under intensive vegetable production from Ultisols in southern China. Results showed that biochar amendment significantly decreased cumulative N 2 O emissions by 40–66 % during the whole study period (p < 0.01), whereas effects on CH 4 emissions were inconsistent across the two study years. In addition, biochar amendment significantly decreased PLFAs and fungi/bacteria ratio by 4–31 % and 12–39 %, respectively. Annual global warming potential (GWP) was effectively reduced by biochar amendment, and random forest analysis showed that soil pH, C/N, humic acid (HA) and fungal PLFAs exhibited the highest mean predictor importance. The nonlinear correlations between the biochar amendment rate and GHG emissions mainly caused by soil pH, and the 20 Mg ha−1 biochar amendment rate was recommended because it produced the lowest GWP with the lowest economic costs. These findings demonstrated that various biochar amendment rates can differently alter GHG emissions and microbial properties under intensive vegetable production, which can provide new insights on optimal biochar amendment rates for GHG mitigation in agricultural production in China. [Display omitted] • Nonlinear responses of GHG mitigation to biochar rates were detected. • Biochar decreased soil bacterial, fungal and total PLFAs. • Biochar increased CH 4 , but reduced N 2 O and annual GWP. • GWP reduction mainly induced by soil pH, C/N, humic acid and fungal PLFAs. [ABSTRACT FROM AUTHOR]

Recently, dc electric springs (dc-ESs) have been proposed to realize voltage regulation and power quality improvement in dc microgrids. This paper establishes a distributed cooperative control framework for multiple dc-ESs in a dc microgrid and presents the small-signal stability analysis of the system. The primary level implements a droop control to coordinate the operations of multiple dc-ESs. The secondary control is based on a consensus algorithm to regulate the dc-bus voltage reference, incorporating the state-of-charge (SOC) balance among dc-ESs. With the design, the cooperative control can achieve average dc-bus voltage consensus and maintain SOC balance among different dc-ESs using only neighbor-to-neighbor information. Furthermore, a small-signal model of a four dc-ESs system with the primary and secondary controllers is developed. The eigenvalue analysis is presented to show the effect of the communication weight on system stability. Finally, the effectiveness of the proposed control scheme and the small-signal model is verified in an islanded dc microgrid under different scenarios through simulation and experimental studies. [ABSTRACT FROM PUBLISHER]

In this study, pH-responsive microspheres loaded with multiple antioxidants were developed for intestine-specific delivery and exhibited synergistic activity. They consist of chitosan (CS)-coated microspheres made of TEMPO-oxidized Konjac glucomannan (OKGM) polymers, of which the carboxyl (COO − ) groups are cross-linked via ferric ions (Fe 3+ ), allowing the hydrophobic (β-carotene) and hydrophilic (anthocyanins) antioxidants to be simultaneously incorporated. CLSM images showed successful co-encapsulation of β-carotene and anthocyanins. The in vitro release kinetics of co-loaded CS-OKGM microspheres in simulated GI fluids indicated that the microspheres retain the dual antioxidants in an acidic gastric environment and release them at intestinal pH. Free radical scavenging experiments demonstrated that multiple antioxidants loaded into OKGM system had synergistic activity with enhanced stability against heat. The multi-functional CS-OKGM microspheres showed great potential for multiple antioxidants and intestine-specific delivery with enhanced stability. [ABSTRACT FROM AUTHOR]

A Pickering (o/w) emulsion was formed and stabilized by whey protein isolate nanoparticles (WPI NPs). Those WPI NPs were prepared by thermal cross-linking of denatured WPI proteins within w/o emulsion droplets at 80 °C for 15 min. During heating of w/o emulsions containing 10% (w/v) WPI proteins in the water phase, the emulsions displayed turbid–transparent–turbid phase transitions, which is ascribed to the change in the size of the protein-containing water droplets caused by thermal cross-linking between denatured protein molecules. The transparent stage indicated the formation of WPI NPs. WPI NPs of different sizes were obtained by varying the mixing speed. WPI NPs of 200–500 nm were selected to prepare o/w Pickering emulsions because of their good stability against coalescence. By Confocal Laser Scanning Microscopy, it was observed that WPI NPs were closely packed and distributed at the surface of the emulsion droplets. By measuring water contact angles of WPI NPs films, it was found that under most conditions WPI NPs present good partial wetting properties, but that at the isoelectric point (p I ) and high ionic strength the particles become more hydrophobic, resulting in less stable Pickering emulsion. Thus, at pH above and below the p I of WPI NPs and low to moderate ionic strengths (1–10 mM), and with a WPI NPs concentration of 2% (w/v), a stable Pickering emulsion can be obtained. The results may provide useful information for applications of WPI NPs in environmentally friendly and food grade applications, notably in food, pharmaceutical and cosmetic products. [ABSTRACT FROM AUTHOR]

Cerebral metabolic dysfunction is a critical pathological hallmark observed in the aftermath of traumatic brain injury (TBI), as extensively documented in clinical investigations and experimental models. An in‐depth understanding of the bioenergetic disturbances that occur following TBI promises to reveal novel therapeutic targets, paving the way for the timely development of interventions to improve patient outcomes. The 13C isotope tracing technique represents a robust methodological advance, harnessing biochemical quantification to delineate the metabolic trajectories of isotopically labeled substrates. This nuanced approach enables real‐time mapping of metabolic fluxes, providing a window into the cellular energetic state and elucidating the perturbations in key metabolic circuits. By applying this sophisticated tool, researchers can dissect the complexities of bioenergetic networks within the central nervous system, offering insights into the metabolic derangements specific to TBI pathology. Embraced by both animal studies and clinical research, 13C isotope tracing has bolstered our understanding of TBI‐induced metabolic dysregulation. This review synthesizes current applications of isotope tracing and its transformative potential in evaluating and addressing the metabolic sequelae of TBI. [ABSTRACT FROM AUTHOR]

The hybrid energy storage system (HESS) plays an essential role in the shipboard medium-voltage dc (MVdc) system to provide backup power, buffer large load change, as well as improve power quality. In this paper, a novel virtual resistor and capacitor droop (VRCD) control is first proposed for HESS including battery, supercapacitor, and flywheel. It enables the generator and multiple types of energy storages (ESs) with different characteristics to respond to multiple-frequency unbalanced power instead of simple high-frequency and low-frequency power sharing by the conventional droop control method. A detailed design procedure for the VRCD control is presented. Also, the secondary state of charge recovery control is proposed to maintain high energy reserve of ESs for further power demand. Simulations are conducted to demonstrate the effectiveness of the control scheme proposed in a shipboard MVdc under particular loads fluctuation, such as the propulsion motor and the pulsed load. [ABSTRACT FROM AUTHOR]

Probiotics have been increasingly investigated for their role in alleviating symptoms of ulcerative colitis (UC), but the specific mechanism involved remains unclear. We investigated the alleviating effect of Bifidobacterium animalis A6 (BAA6) in UC through a mouse dextran sulfate sodium (DSS) model. When treated with a high dose of BAA6 (1 × 1010 cfu/ml), it was found that colitis symptoms were significantly alleviated, and mucosal damages experienced obvious relief. Moreover, a high dose of BAA6 effectively upregulated free fatty acid receptors 2 and 3 (FFAR2 and FFAR3) expression and butyric acid metabolism specifically. Furthermore, the supplement of BAA6 significantly suppressed pro‐inflammatory cytokines levels (interleukin‐13) and the expression of pore‐forming protein claudin‐2. The upstream regulatory genes of claudin‐2, such as STAT6, GATA4, Cdx2, were also significantly inhibited by BAA6. Collectively, this study concludes that BAA6 attenuated DSS‐induced colitis by increasing the levels of intestinal butyric acid, activating the butyric acid‐FFAR pathway, suppressing excessive proinflammatory response, and protecting the function of the colon epithelial barrier. [ABSTRACT FROM AUTHOR]

β-Carotene is a natural antioxidant that is beneficial for human health. β-Carotene is well known for its aqueous insolubility and its sensitivity to environmental stimuli. To achieve the targeted delivery of β-Carotene to human intestine, a microgel system was developed. The microgel is made of oxidized potato starch polymers, which are obtained by chemical cross-link process by sodium trimetaphosphate (STMP). At pH < p I (WPI: pH 5), as in the acidic condition in stomach, the positively charged WPI emulsified β-Carotene nanoemulsion droplets were absorbed by the negatively charged starch microgel particles, which prevented the early release of β-Carotene in stomach. At pH > p I , as in the intestinal condition, WPI–β-Carotene nanoemulsion droplets and microgel particles both carry negative charges. Under this condition, β-Carotene will be released from microgel. In this study, we investigated the absorption capacity of β-Carotene nanoemulsion droplets by oxidized starch microgel particles of various degrees of oxidation (DO) and the cross-link density ( R cross-linker/polymer(w/w) ) is investigated as a function of pH and salt concentrations. We found that DO30% with R cross-linker/polymer(w/w) = 0.1 was the optimal gel type for nanoemulsion droplets absorption, and pH 3 and ionic strength of 0.06 M were the optimal conditions for nanoemulsion droplets absorption. The swelling capacity of the microgel particles rather than its ζ-potential was dominant in governing the absorption capacity. The in vitro release experiment under stimulated gastrointestinal fluids suggested that β-Carotene emulsions droplets remained stable at the gastric condition and were majorly released under the intestinal condition. The results suggested that the oxidized starch microgel could be used to prevent the early release of β-Carotene in the stomach and target delivery of them to the intestine. [ABSTRACT FROM AUTHOR]

Recent events have shown that cyber attacks are becoming real threats to the power system reliability and resiliency, and there is no exception to the DC microgrids which would become popular in the future. Currently, most secondary controls in DC microgrids heavily rely on communications, making them specifically vulnerable to cyber attacks. This paper presents a resilient distributed control based on the average consensus algorithm and the proportional-integral (PI) consensus algorithm to enhance the system resilience against denial of service attacks and improve the microgrid stability, particularly in the presence of constant power loads. The proposed control casts the average voltage restoration problem into an optimization problem, and because of this, the voltage control is immune to the communication damage caused by malicious denial of service attacks. Moreover, the small-signal stability model of the DC microgrid with distributed energy storages and constant power loads is established, and the impacts of varying constant power loads, controller parameters and communication topology on the system stability are analyzed. Simulations are conducted to verify the effectiveness of the proposed method. • Global voltage and current regulations are realized by distributed mechanisms. • Distributed controllers can keep the DC microgrids stable during cyber-attacks. • Proportional-integral consensus based controllers can recover from Denial of Service attacks. • Distributed controllers contribute to improving stability in the presence of constant power loads. [ABSTRACT FROM AUTHOR]

• BCAAs can be regarded as serum biomarkers for obesity. • Luffa potentially suppressed bacterial BCAA biosynthesis. • Luffa -affected bacterial metabolites triggered tissue-specific BCAA catabolism. • Luffa improved circulating BCAA dysfunction via gut microbiota to attenuate obesity. High circulating branched-chain amino acid (BCAA) levels can be diagnosis indicators for obesity. Luffa cylindrica (luffa) is one of vegetables against obesity. However, whether the anti-obesity of luffa is associated with BCAA metabolism and gut microbiota remains unknown. Here, we used conventionally raised diet-induced obese (DIO) mice to prove dietary luffa could reduce higher circulating BCAA levels and upregulate the tissue-specific expressions of BCAA-catabolizing enzymes. Meanwhile, dietary luffa selectively decreased the relative abundances of g_Enterortabdus , g_Eubacterium_xylanophilum_group and g_Butyricicoccus that exhibited significantly positive correlations with BCAA levels, BMI and HOMA-IR. Bacterial functionality prediction indicated dietary luffa potentially inhibited bacterial BCAA biosynthesis for reducing BCAAs supplementation. More importantly, dietary luffa had no impacts on BCAA catabolism in germ-free-mimic DIO mice. Thus, dietary luffa improved BCAA dysfunction via gut microbiota to attenuate obesity. This study offers a novel insight into dietary intervention against obesity from the aspect of gut microbiota-amino acid metabolism. [ABSTRACT FROM AUTHOR]

Luffa cylindrica is a nutrient-dense vegetable with medical properties and can alleviate metabolic diseases. Numerous evidences demonstrated gut microbiota impacted the progress of nonalcoholic fatty liver disease (NAFLD). This study was to investigate the underlying mechanism of L. cylindrica supplementation against NALFD via gut microbiota from hepatic transcriptional and metabolic analysis. In diet-induced obese mice, we observed L. cylindrica supplementation (2 g/kg body weight) effectively alleviated high-fat diet-induced obese symptoms such as body weight, fat deposition, and insulin resistance. Notably, L. cylindrica supplementation significantly relieved hepatic steatosis and inflammation infiltration to decrease hepatic toxicity. RNA-sequencing analysis showed that 130 hepatic genes in total significantly altered responding to L. cylindrica supplementation. And signaling pathway analysis revealed that L. cylindrica supplementation down-regulated the transcriptional expressions of CD36 and Rxrg to inhibit hepatic lipid synthesis. Moreover, L. cylindrica supplementation increased the transcriptional expressions of Ass1, Cps1, Cth, Got1, Tat, and Gls2 to enhance amino acid levels (Gly, Ala, Pro, Val, Ile, Asn, Met, and Phe) and improve hepatic abnormal gluconeogenesis. Furthermore, in antibiotic-treated obese mice, L. cylindrica supplementation did not change these gene expressions along with the hepatic levels of lipid and amino acids. Taken together, L. cylindrica supplementation could effectively suppress hepatic steatosis in diet-induced obese mice through inhibiting lipid synthesis and enhancing amino acid levels in liver, which depended on gut microbiota. Thus, L. cylindrica might be one promising dietary supplementation targeting at gut microbiota to reduce NAFLD risk. [ABSTRACT FROM AUTHOR]

Unregulated charging of a large number of electric vehicles (EVs) simultaneously may cause electricity shortages and price spikes in the power market. This paper deals with the optimization of EV charging strategy using the priority sequence. Firstly, the evaluation indices such as the schedulable interval ratio, the emergency probability and the battery losses are proposed. Then a comprehensive evaluation system for the EV scheduling priority is established by adopting the entropy weight method to incorporate multiple indices. Based on the scheduling priority obtained, a double-hierarchical optimal model is proposed, taking into account the constraints such as the demand of the EV owner. Its upper objective aims to minimize the sum of the square of deviation between the actual and the required schedulable capacity of EV aggregator over every interval. The lower one minimizes the sum of EV scheduling priority sequence over the scheduling interval. Case studies with 100 EVs show that the hierarchical optimization model can assist EV aggregator in making effective charging scheme. It is also observed that better flexibility for dispatching EVs can be achieved using multiple indices with weights. [ABSTRACT FROM AUTHOR]

With the increasing popularity and rapid development of portable and wearable electronics, it has become extremely crucial to employ electromagnetic interference (EMI) shielding materials that efficiently reduce the ensuing radiation pollution. Nonetheless, the substantial thickness and poor mechanical properties of most EMI-shielding materials have hindered their implementation in portable and wearable electronics. Herein, a facile alternating vacuum-assisted filtration strategy was used to fabricate an ultrathin and flexible multilayer MXene/WPU film for EMI shielding applications. It possesses excellent mechanical properties, including a tensile strength of 71.11 MPa, a toughness of 1.79 MJ m−3, and a strain at failure of 5.46%. The alternating multilayer MXene/WPU film enhanced the EMI shielding performance, which exhibits a 52 dB EMI shielding efficiency (SE) and a thickness specific SE of 6407.97 dB cm2 g−1 with a thickness of 35 µm. In addition, as synthesized composite film possesses high electrical conductivity of 487.95 S m−1. This strategy is essential for preparing ultrathin, flexible composites with high EMI shielding capability and expanding the practical applications of composites based on MXene. [Display omitted] • Ultrathin and flexible multilayer MXene/WPU films were made by alternating VAF. • The EMI SE achieves 52 dB and SSE/t is 6407.97 dB cm2 g−1 at a thickness of 35 µm. • Specific multilayer structures enhance reflection and internal multiple reflection. • The tensile strength and toughness are 71.11 MPa and 1.79 MJ m−3 respectively. [ABSTRACT FROM AUTHOR]

Highlights • A virtual capacitor droop control (CDC) using fuzzy logic algorithm in multiple HESSs can adaptively adjust the droop coefficient, so as to eliminate the impact of the line impedance on the power splitting and improve the power sharing accuracy. This study has not been reported in previous work. • Both the small-signal model and eigenvalue analysis are presented to study the stability of the HESS system using CDC, investigate the impact of the droop coefficient variation on the system dynamics and determine an optimal range of the modified droop coefficient. This work has not been involved in the previous studies. Abstract In a DC microgrid, virtual capacitor droop control (CDC) can achieve power allocation according to different frequency characteristics of a hybrid energy storage system (HESS). However, the control performance may be easily affected by the line impedance. In this paper, an adaptive droop controller based on a fuzzy logic algorithm is proposed. By detecting the unbalanced power and the voltage deviation, the droop coefficient can be adaptively adjusted to mitigate the influence of the line impedance and achieve desirable power splitting in the HESS. Furthermore, the small-signal model of the HESS is established and the impact of the droop coefficient variation on the stability of the DC microgrid is exploited. The simulation results in PSCAD/EMTDC verify the effectiveness of the proposed adaptive droop controller. It has been proved that desirable power splitting can be obtained among multiple HESSs under different scenarios. [ABSTRACT FROM AUTHOR]

TEMPO-oxidized Konjac glucomannan (OKGM) was developed as new material for preparing vegetarian hard capsules. OKGM of different degrees of oxidation: DO30%, DO50%, and DO80% were prepared to select optimum DO for capsule formation. FT-IR results proved that the primary alcohol groups on KGM were oxidized into carboxyl groups. XRD analysis suggested that TEMPO-oxidation decreased the crystallinity of KGM. DO80% was considered as the optimum candidate for capsule preparation owing to its superior solubility, transparency and reduced viscosity. The hydrophilicity of OKGM films, measured by contact angle measurement, increased with increasing DO. The elongation at break and tensile strength of the OKGM films enhanced with increasing DO. In vitro drug dissolution profile of OKGM capsules showed that the shell rupture time of DO80% capsule is about 5–10 min, and 80% of the drugs were released within 30–45 min. Thus DO80% OKGM was qualified to be used for gastric soluble hard capsules. [ABSTRACT FROM AUTHOR]

A novel oxidized konjac glucomannan (OKGM) microsphere system was developed to protect and targeted deliver the sensitive antioxidant–anthocyanins into intestine. The microspheres were made of oxidized konjac glucomannan, which was cross-linked by Fe 3+ via emulsification method. The absorption of anthocyanins was driven by the electrostatic interaction between the negatively charged OKGM microspheres and positively charged anthocyanins. CLSM images showed that anthocyanins distributed homogenously through the OKGM microspheres. The absorption and release behavior of anthocyanins on OKGM microspheres were determined as a function of pH and salt concentration. A maximum absorption capacity was obtained at pH 3 and low salt concentration. However, anthocyanins were released significantly at simulated stomach conditions due to fast degradation of OKGM microspheres. An extra positively charged chitosan oligosaccharides (COS) layer was applied on OKGM microspheres to prevent H + ions permeating into microspheres under stomach condition. COS oligomers were cross-linked with glutaraldehyde to further strengthen the OKGM microspheres' structure. Finally, glutaraldehyde cross-linked COS stabilized OKGM microspheres became stable against H + ions in simulated stomach condition, therefore OKGM microspheres can prevent an early release of the anthocyanins in stomach condition and achieve a sustained released in the intestinal condition. The in vitro release experiments revealed that the OKGM microspheres had a potential as a carrier of targeted delivery of bioactive compounds in the intestine. [ABSTRACT FROM AUTHOR]

A biodegradable microgel system based on glycerol-1,3-diglycidyl ether (GDGE) cross-linked TEMPO-oxidized potato starch polymers was developed for controlled uptake and release of proteins. A series of microgels were prepared with a wide range of charge density and cross-link density. We found both swelling capacity ( SW w ) and lysozyme uptake at saturation ( Γ sat ) increased with increasing degree of oxidation (DO) and decreasing cross-link density. Microgel of DO100% with a low cross-link density (R GDGE/polymer (w/w) of 0.025) was selected to be the optimum gel type for lysozyme absorption; Γ sat increased with increasing pH and decreasing ionic strength. It suggests that the binding strength was the strongest at high pH and low ionic strength, which was recognized as the optimum absorption conditions. The lysozyme release was promoted at low pH and high ionic strength, which were considered to be the most suitable conditions for triggering protein release. These results may provide useful information for the controlled uptake and release of proteins by oxidized starch microgels. [ABSTRACT FROM AUTHOR]