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In this study, five phage display antibodies (pdAbs) against ectromelia virus (ECTV) were selected from vaccinia virus (VACV)-immune phage-display library of human single chain variable fragments (scFv). ELISA demonstrated that selected pdAbs could recognize ECTV, VACV, and cowpox virus (CPXV). Atomic force microscopy visualized binding of the pdAbs to VACV. Three of the selected pdAbs neutralized variola virus (VARV) in the plaque reduction neutralization test. Western blot analysis of ECTV, VARV, VACV, and CPXV proteins indicated that neutralizing pdAbs bound orthopoxvirus 35 kDa proteins, which are encoded by the open reading frames orthologous to the ORF H3L in VACV. The fully human antibody fh1A was constructed on the base of the VH and VL domains of pdAb, which demonstrated a dose-dependent inhibition of plaque formation after infection with VARV, VACV, and CPXV. To determine the p35 region responsible for binding to neutralizing pdAbs, a panel of truncated p35 proteins was designed and expressed in Escherichia coli cells, and a minimal p35 fragment recognized by selected neutralizing pdAbs was identified. In addition, peptide phage-display combinatorial libraries were applied to localize the epitope. The obtained data indicated that the epitope responsible for recognition by the neutralizing pdAbs is discontinuous and amino acid residues located within two p35 regions, 15-19 aa and 232-237 aa, are involved in binding with neutralizing anti-p35 antibodies., (Copyright © 2018. Published by Elsevier B.V.)

The problem of step-by-step control of radiotherapy of malignant tumors was solved on the basis of Ellis' concept, widely used in radiology. A new method for determining step parameters was proposed. An algorithm and an example of the calculation of parameters of a course of therapy in case of a heterogeneous tumor were given.

A total of 128 patients with alimentary obesity were examined in the course of the treatment. There was a significant elevation in blood serum of total and free cholesterol, its esters, free fatty acids, beta-lipoproteins, and a decrease in the activity of serum lipase. The correlation of cholesterol esters to phospholipids was found to be disordered. Eighty patients demonstrated, after treatment with chloride sodium bromoiodine baths combined with hypocaloric diet and exercise therapy, a favourable clinical time course and normalization of the majority of lipid metabolism indicators, with an increase in serum lipase activity. Twenty-five patients, who received fresh baths under similar conditions, demonstrated less pronounced shifts in lipid metabolism, which were not always significant. The treatment complex including phepranon also gave rise to the normalization of the majority of lipid metabolism indicators. However, one-third of cases developed serious side effects.