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The role of the FCA promoter in the regulation of flowering time in arabidopsis plants was analyzed via X-ray mutagenesis. The arabidopsis fca-mutants exhibited impaired plant development during the vegetative and reproductive stages. The transcript gamma was also characterized as the genetic factor that encodes the FCA protein which promoted the posttranscriptional cascade that regulated flowering time in arabidopsis.

• The Zn/Cd-transporting ATPase, HMA2, has N- and C-terminal domains that can bind Zn ions with high affinity. Mutant derivatives were generated to determine the significance of these domains to HMA2 function in planta. • Mutant derivatives, with and without a C-terminal GFP tag, were expressed from the HMA2 promoter in transgenic hma2,hma4, Zn-deficient, plants to test for functionality. • A deletion mutant lacking the C-terminal 244 amino acids rescued most of the hma2,hma4 Zn-deficiency phenotypes with the exception of embryo or seed development. Root-to-shoot Cd translocation was fully rescued. The GFP-tagged derivative was partially mis-localized in the root pericycle cells in which it was expressed. Deletion derivatives lacking the C-terminal 121 and 21 amino acids rescued all phenotypes and localized normally. N-terminal domain mutants localized normally but failed to complement the hma2,hma4 phenotypes. • These observations suggest that the N-terminal domain of HMA2 is essential for function in planta while the C-terminal domain, although not essential for function, may contain a signal important for the subcellular localization of the protein. [ABSTRACT FROM AUTHOR]

Investigates use of gene knockouts to investigate Arabidopsis thaliana metabolism. Accounts on knockouts of genes encoding enzymes of primary metabolism; Limitations of gene silencing approaches; Details on primary metabolism from the study of knockouts.