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Plants are thought to lack an early segregating germline and often retain both asexual and sexual reproduction, both of which may allow somatic mutations to enter the gametes or clonal progeny, and thereby impact plant evolution. It is yet unclear how often these somatic mutations occur during plant development and what proportion is transmitted to their sexual or cloned offspring. Asexual "seedless" propagation has contributed greatly to the breeding in many fruit crops, such as citrus, grapes and bananas. Whether plants in these lineages experience substantial somatic mutation accumulation is unknown. To estimate the somatic mutation accumulation and inheritance among a clonal population of plant, here we assess somatic mutation accumulation in Musa basjoo, a diploid banana wild relative, using 30 whole-genome resequenced samples collected from five structures, including leaves, sheaths, panicle, roots and underground rhizome connecting three clonal individuals. We observed 18.5 high proportion de novo somatic mutations on average between each two adjacent clonal suckers, equivalent to ~ 2.48 × 10

The inhibition effect of three inhibitors with different chain lengths of their ether groups on Q235 steel in 0.5 M HCl solution at 318 K was determined by means of weight loss measureme nt, electrochemical methods (Tafel and EIS) and surface analysis (SEM). Moreover, the further study of corrosion inhibition mechanism was also conducted by computational methods (Quantum chemical calculations and Molecular dynamics simulations). Results reveal that the three inhibitors exhibit an excellent inhibition performance for carbon steel, and the corrosion inhibition efficiency of the three inhibitors increases with the increase of the chain length, which favors the formation of a protection film adsorbed on the surface. Analysis of polarization data informs that the adsorption type of three inhibitors basically obeys the Langmuir monolaye r adsorption, which is mainly of chemisorptive nature. Computational methods also tell that the three inhibitors possess a high reactivity and strong interaction with the iron surface, furthermore, the interaction is increased with the increase of the chain length of three inhibitors. The conclusion is in good agreement with the experimental results.

As hypoxia is an important factor to limit chemotherapeutic efficacy in tumors, we herein report three ruthenium(II)-arene complexes containing a hypoxia inducible factor-1α inhibitor (YC-1), which endow the organometallic complexes with potential for hypoxia targeting. In vitro tests showed the resulting complexes had higher anticancer activities in hypoxia than in normoxia against the tested cancer cell lines. Western blot analysis revealed that complexes 1-3 blocked HIF-1α protein accumulation under hypoxic conditions. Moreover, these complexes displayed much less cytotoxicity toward the normal human umbilical vein endothelial cell line (HUVEC), indicating that complexes 1-3 may be selectively cytotoxic for human cancer cell lines. These findings proved that ligation with YC-1 endowed these organometallic ruthenium(II) complexes with potential for hypoxia targeting in addition to enhancing their anticancer activities.