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With Chinese President Xi’s commitment to “carbon peak and carbon neutrality” to the international community at the 75th United Nations General Assembly, the teaching of practical courses of construction engineering management has faced major challenges. In view of the current problems that the setting of practice course content and evaluation indicators in the training program of construction engineering cannot effectively deal with the " carbon peaking and carbon neutrality goals ", this paper puts forward reform strategies of curriculum teaching methods such as the “four stage, full cycle progressive green building practical ability training system”, and the "multi module practical" green building core teaching system, and the "online + offline" integrated teaching mode exploration, and diversified curriculum evaluation mechanism, etc. This paper also makes appropriate adjustments from the aspects of professional practice teaching content setting, teaching methods and evaluation mechanism, so as to meet the new requirements of "carbon peaking and carbon neutrality goals" for construction engineering management majors.

Granaticin is a kind of antibiotics with the function of anticancer, antibacterial, anticoccidial. Streptomyces sp. A1013Y is a new strain isolated from soil with granaticin-producing ability. The genome information was analyzed for the further study of granaticin. The present work reported the complete genome of S. sp. A1013Y, which contains a 7,646,296 bp chromosome with an average GC content of 71.59%. A total of 7361 CDSs, including 66 tRNA genes, 18 rRNA genes and 23 clusters, were identified in the genome. With homologous recombination, an in-frame deletion mutant was constructed to confirm the granaticin cluster. The fermentation liquid of the mutant did not contain granaticin based on HPLC and LC-MS analysis, indicating that S. sp. A1013Y is a granaticin producer. Comparing the granaticin biosynthesis cluster of S. sp. A1013Y with Streptomycesviolaceoruber Tü22 and Streptomyces vietnamensis GIMV4.0001T, which were sequenced and produce granaticin, the similarity between the granaticin clusters was 78% and 83% respectively, but some genes still weren’t identified in the granaticin biosynthesis cluster. The phylogenetic analysis and ANI value between S. sp. A1013Y and other Streptomyces species, including S. vietnamensis GIMV4.0001T, were all below 85%, which showed that S. sp. A1013Y was probably a novel streptomyces strain that produced granaticin. The whole genome information of S. sp. A1013Y provides a valuable foundation for future granaticin analyses as well as biomedicine applications.

A selective analytical method based on packed-fiber solid-phase extraction and ultra-high performance liquid chromatography–tandem mass spectrometry (PFSPE-UPLC–MS/MS) has been developed for determination of six β-agonists (clorprenaline, bambuterol, clenbuterol, brombuterol, mabuterol, and penbuterol) in pork tissue. Polystyrene-polymeric crown ether (PS-PCE) composite nanofibers were fabricated by electrospinning and utilized to prepare the homemade extraction columns. With optimal conditions, all analytes were separated very well and the blank pork did not disturb the determination, and the linearity is good in a range of 5.0 μg/kg–25.0 μg/kg. The recoveries were 79.3–110.1%. RSDs for intra-day were in the range of 1.5–10.5% and RSDs for inter-day were 4.7–11.8%. Above all, only 5 mg of sorbent and 200 μL of elution solvent were favorable to directly extract all analytes in a complex matrix. The method is simple and cost-effective, and has the potential to be applied to quantitatively analyze the concentrations of polar species in food samples containing complex matrix.

A novel blue pigment was first isolated from Streptomyces sp. A1013Y. The purified component was identified as 4,8,13-trihydroxy-6,11-dione-trihydrogranaticins A (TDTA). Its physical properties were found to be: Molecular weight 462 Da; Color value, E0.1%1cm580 nm = 80; Solubility, it dissolved in organic solvents. In addition, the color of TDTA changed with pH but was found to be relatively stable between 20 and 100 °C, from pH 3 to pH 11, and under UV-light or darkness. TDTA’s functional properties was as follows: TDTA showed excellent free radical scavenging properties, IC50 41.04 µg/mL and 13.75 µg/mL using 2, 20-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-n-(3, 2-ethyl-benzothiazole-6-sulfonic acid) ammonium salt (ABTS) respectively. TDTA might be a promising source of natural pigment and bioactive compound used as additive in food industry.

Plant reoviruses are thought to replicate and assemble within cytoplasmic, nonmembranous structures called viroplasms. Here, we established continuous cell cultures of the white-backed planthopper ( Sogatella furcifera Horváth) to investigate the mechanisms for the genesis and maturation of the viroplasm induced by Southern rice black-streaked dwarf virus (SRBSDV), a fijivirus in the family Reoviridae , during infection of its insect vector. Electron and confocal microscopy revealed that the viroplasm consisted of a granular region, where viral RNAs and nonstructural proteins P6 and P9-1 accumulated, and a filamentous region, where viral RNAs, progeny cores, viral particles, as well as nonstructural proteins P5 and P6 accumulated. Our results suggested that the filamentous viroplasm matrix was the site for the assembly of progeny virions. Because viral RNAs were produced by assembled core particles within the filamentous viroplasm matrix, we propose that these viral RNAs might be transported to the granular viroplasm matrix. P5 formed filamentous inclusions and P9-1 formed granular inclusions in the absence of viral infection, suggesting that the filamentous and granular viroplasm matrices were formed primarily by P5 and P9-1, respectively. P6 was apparently recruited in the whole viroplasm matrix by direct interaction with P9-1 and P5. Thus, the present results suggested that P5, P6, and P9-1 are collectively required for the genesis and maturation of the filamentous and granular viroplasm matrix induced by SRBSDV infection. Based on these results, we propose a new model to explain the genesis and maturation of the viroplasms induced by fijiviruses in insect vector cells.

Solid phase extraction (SPE) has emerged as the widely used technique for sample preparation in the analytical process. Recent research trends of SPE are toward developing novel adsorbents to enrich the analytes simply and effectively. In this study, we proposed the poly-3,4-ethylenedioxythiophene (PEDOT) nanoclusters as the SPE adsorbent. During the application, only a small amount of PEDOT nanoclusters was needed and placed in a pipet tip with glass wool on either side. Without complex preparation, the target analytes could be directly extracted from the sample onto the extraction material and eluted in this lab-in-a-pipet-tip system. The efficiency of this approach was demonstrated by detecting 20 kinds of sulfonamides (SAs) in honey with ultra-performance liquid chromatography/tandem mass spectrometry (UPLC–MS/MS). All the analytes were detected by multiple reaction monitoring (MRM) mode. The established method was extensively validated by determining the linearity (R2 ≥ 0.991), average recovery (88.4–105.0%) and precision (relative standard deviation ≤9.60%). Low detection limits (0.5–4 μg kg−1), wide linearity (10–250 μg kg−1) and short sample pretreatment time (20 min) were achieved under the optimized conditions. The absolute recoveries of the SAs at high level ranged from 71.1% to 91.4%. Due to its simplicity, selectivity and sensitivity, our new method has potential applications in quantitative analysis of the target compounds in complex samples.