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Abstract Background Stereotactic ablative body radiotherapy (SABR) is an emerging treatment for patients with primary renal cell carcinoma (RCC). However, its impact on renal function is unclear. This study aimed to evaluate incidence and clinical factors predictive of severe to end-stage chronic kidney disease (CKD) after SABR for RCC. Methods and materials This was a Single institutional retrospective analysis of patients with diagnosed primary RCC receiving SABR between 2012–2020. Adult patients with no metastatic disease, baseline estimated glomerular filtration rate (eGFR) of ≥ 30 ml/min/1.73 m2, and at least one post-SABR eGFR at six months or later were included in this analysis. Patients with upper tract urothelial carcinoma were excluded. Primary outcome was freedom from severe to end-stage CKD, determined using the Kaplan–Meier estimator. The impact of baseline CKD, age, hypertension, diabetes, tumor size and fractionation schedule were assessed by Cox proportional hazard models. Results Seventy-eight consecutive patients were included, with median age of 77.8 years (IQR 70–83), tumor size of 4.5 cm (IQR 3.9–5.8) and follow-up of 42.2 months (IQR 23–60). Baseline median eGFR was 58 mls/min; 55% (n = 43) of patients had baseline CKD stage 3 and the remainder stage 1–2. By last follow-up, 1/35 (2.8%) of baseline CKD 1–2, 7/27 (25.9%) CKD 3a and 11/16 (68.8%) CKD 3b had developed CKD stage 4–5. The estimated probability of freedom from CKD stage 4–5 at 1 and 5 years was 89.6% (CI 83.0–97.6) and 65% (CI 51.4–81.7) respectively. On univariable analysis, worse baseline CKD (p

BCR-ABL TKIs are the standard-of-care treatment for chronic myelogenous leukemia (CML). While several side-effects of these TKIs are well-known or included on the FDA label, renal adverse drug reactions are not. We recently observed 2 patients with proteinuria and acute kidney injury (AKI) not attributable to other causes that resolved once stopping the BCR-ABL TKI they were receiving. Further investigation into renal ADRs and pre-disposing factors, the impact of dose and duration of treatment on frequency of ADRs, and the extent and permanence of sequelae would help hematologists/oncologists select the best therapy and monitor for ADRs in high-risk patients.

E Du,1,* Chao Lu,1,* Fei Sheng,1 Changying Li,1 Hong Li,2 Na Ding,1 Yue Chen,1 Ting Zhang,1 Kuo Yang,1 Yong Xu1 1Central Laboratory, Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, Tianjin 300211, People’s Republic of China; 2The Institute of Molecular Cardiology, Medical school, University of Louisville, Louisville, KY, USA *These authors contributed equally to this work Background: Dysfunction of primary cilia (PC), which could influence cell cycle and modulate cilia-related signaling transduction, has been reported in several cancers. However, there is no evidence of their function in bladder cancer (BLCA). This study was performed to investigate the presence of PC in BLCA and to explore the potential molecular mechanisms underlying the PC in BLCA.Patients and methods: The presence of PC was assessed in BLCA and adjacent non-cancerous tissues. The gene expression dataset GSE52519 was employed to obtain differentially expressed genes (DEGs) associated with PC. The mRNA expression of the DEGs were confirmed by Gene Expression Profiling Interactive Analysis. The DEGs properties and pathways were analyzed by Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis. Genomatix software was used to predict putative transcription factor binding sites (TFBS) in the promoter region of DEGs, and the transcription factors were achieved according to the shared TFBS, which were supported by the ChIP-Sequence data.Results: PC were found to be reduced in BLCA tissue samples in this study. Seven DEGs were observed to be associated with PC, and gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that these DEGs exhibited the properties and functions of PC, and that the Hedgehog signaling pathway probably participated in the pathogenesis and progression of BLCA. The mRNA expression of the seven DEGs in 404 BLCA and 28 normal tissue samples were analyzed, and five DEGs including CENPF, STIL, AURKA, STK39 and OSR1 were identified. Five TFBS including CREB, E2FF, EBOX, ETSF and HOXF in the promoter region of five DEGs were calculated and the transcription factors were obtained according to the shared TFBS.Conclusion: PC were found to be reduced in BLCA, and the potential molecular mechanisms of PC in BLCA helped to provide novel diagnosis and therapeutic targets for BLCA. Keywords: bladder cancer, primary cilia, differentially expressed genes, microarray analysis, transcription factors

Measurable residual disease is associated with inferior outcomes in patients with acute myeloid leukemia (AML). Measurable residual disease monitoring enhances risk stratification and may guide therapeutic intervention. The European LeukemiaNet working party recently came to a consensus recommendation incorporating leukemia associated immunophenotype-based different from normal approach by multi-color flow cytometry for measurable residual disease evaluation. However, the analytical approach is highly expertise-dependent and difficult to standardize. Here we demonstrate that loss of plasmacytoid dendritic cell differentiation after 7+3 induction in AML is highly specific for measurable residual disease positivity (specificity 97.4%) in a uniformly treated patient cohort. Moreover, loss of plasmacytoid dendritic cell differentiation as determined by a blast-to-plasmacytoid dendritic cell ratio >10 was strongly associated with inferior overall and relapse-free survival (RFS) [Hazard ratio 2.79, 95% confidence interval (95%CI): 0.98-7.97; P=0.077) and 3.83 (95%CI: 1.51-9.74; P=0.007), respectively), which is similar in magnitude to measurable residual disease positivity. Importantly, measurable residual disease positive patients who reconstituted plasmacytoid dendritic cell differentiation (blast/ plasmacytoid dendritic cell ratio

In this paper, choosing highly frequent keywords from core journals in the field of 1992-2013 national knowledge discovery in CNKI database, counting the number of two frequent keywords co-occurrences in the same journal, then constructing the highly frequent keywords matrix, and transforming the highly frequent keywords matrix into a correlation matrix and a dissimilarity matrix, we analyze the dissimilarity matrix based on the use of factor analysis, cluster analysis. After discussing the results of the analysis, we found that the current hotspots in the field of domestic knowledge discovery have focused on the following six aspects, knowledge discovery based on data research, knowledge discovery algorithm optimization research, the model of knowledge discovery and references research, knowledge management based on domain ontology, expert system construction research, and applied research of the knowledge discovery. Finally, we summarized the research hotspots in the field of international knowledge discovery in the same way and suggested the domestic scholars to extend some directions of the research in the field of knowledge discovery.

The current study used the human Caco-2 cell line and mouse intestine to explore the topology of expression of the class B type I scavenger receptor (SR-BI) in intestinal cells. Results showed that intestinal cells expressed only the SR-BI isoform with little or no expression of the SR-BII variant. The expression of SR-BI in Caco-2 cells is differentiation dependent, with little or no expression in preconfluent undifferentiated cells. Analysis of Caco-2 cells cultured in Transwell porous membranes revealed the presence of SR-BI on both the apical and basolateral cell surface. Immunoblot analysis of mouse intestinal cell extracts demonstrated a gradation of SR-BI expression along the gastrocolic axis of the intestine, with the highest level of expression in the proximal intestine and decreasing to minimal expression levels in the distal intestine. Immunofluorescence studies with SR-BI-specific antibodies also confirmed this expression pattern. Importantly, the immunofluorescence studies also revealed that SR-BI immunoreactivity was most intense in the apical membrane of the brush border in the duodenum. The crypt cells did not show any reactivity with SR-BI antibodies. The localization of SR-BI in the jejunum was found to be different from that observed in the duodenum. SR-BI was present on both apical and basolateral surfaces of the jejunum villus. Localization of SR-BI in the ileum was also different, with little SR-BI detectable on either apical or basolateral membranes. Taken together, these results suggest that SR-BI has the potential to serve several functions in the intestine. The localization of SR-BI on the apical surface of the proximal intestine is consistent with the hypothesis of its possible role in dietary cholesterol absorption, whereas SR-BI present on the basolateral surface of the distal intestine suggests its possible involvement in intestinal lipoprotein uptake.—Cai, S. F., R. J. Kirby, P. N. Howles, and D. Y. Hui. Differentiation-dependent expression and localization of the class B type I scavenger receptor in intestine.

Arsenic (As) contamination of water can pose a significant threat to human health. Landoltia punctata is a superior water ecological restoration plant that can absorb As and thus manage the problem of As overload in the aquatic environment. Phytohormones play an important role in regulating plant growth and improving heavy metal resistance. In this study, the enrichment effect of phytohormones on As uptake and tolerance mechanism of L. punctata at different concentrations were investigated. The results showed that the application of Indolent-3-acetic (IAA), Abscisic acid (ABA) and Kinetin (KT) were all effective in increasing chlorophyll a (Ca) and carotenoid (Cc) contents. Low concentrations of phytohormones from 0.001 to 1 μmol·L-1 had a facilitative effect on fluorescence parameters in the order of IAA > ABA > KT. The application of high concentrations of phytohormones inhibited the fluorescence parameters. The application of 0.1 μmol·L-1 IAA, ABA and KT treatments were the most effective in promoting As uptake in L. punctata. The effect sequence of different plant hormone on As enrichment was: ABA > IAA ≥ KT. Low concentrations of IAA, ABA and KT treatments all significantly increased superoxide dismutase (SOD) and glutathione reductase (GR) activities, significantly decreased malondialdehyde (MDA) and H2O2 contents and promoted proline synthesis in As-stressed L. punctata. This indicates that the application of phytohormones can improve the antioxidant activity of As-stressed L. punctata, promote the growth of L. punctata and improve its As absorption capacity. This study may provide a scientific basis for phytohormones to promote the remediation of As-contaminated waters by L. punctata, and can provide scientific basis for phytohormones to promote the remediation of Ascontaminated waters by L. punctata. [ABSTRACT FROM AUTHOR]