Search

In this study, 1.5% (m/m) tilapia myofibrillar protein (MP) and corn oil (0%, 10%, 68% or 70%, V/V) were used to prepare emulsions. Molecular dynamics (MD) combined with Raman spectroscopy was used to investigate the structural changes of MP adsorbed at the emulsion interface. MD simulation showed that the spatial conformation of myosin was changed when the protein adsorbed onto the interface of emulsions with different proportions of oil phase; the secondary structure contained about 48% α-helix, and the structure remained relatively stable after the protein adsorbed onto the interface. The change of myosin spatial conformation was accompanied by the increase of solvent accessible surface area (SASA) and the enhancement of hydrophobic interaction, which made the emulsion stable. Raman spectra showed secondary structural changes similar to those of MD. In addition, Raman spectroscopy results showed that tryptophan (Trp) and tyrosine (Tyr) residues were exposed on the protein surface and participated in hydrogen bond formation and hydrophobic interaction, which was conducive to the stability of the emulsion system. In conclusion, changes in the spatial conformation and the microenvironment of side chain groups of MP adsorbed at the oil/water interface of emulsions can promote the hydrogen bond and hydrophobic interaction between protein molecules, and consequently the formation of a tight interfacial film at the oil/water interface, which is conducive to improving the stability of emulsions.

The purpose of this work was to construct liver-targeted nanoparticles based on the redox response to effectively deliver cannabidiol (CBD) for the prevention of acute liver injury (ALI). CBD-loaded nanoparticles (CBD NPs) with a particle size of 126.5 ± 1.56 nm were prepared using the polymer DA-PP-LA obtained by grafting pullulan polysaccharide with deoxycholic acid (DA) and α-lipoic acid (α-LA). CBD NPs showed typical redox-response release behavior. Interestingly, CBD NPs exhibited admirable liver targeting ability, significantly accumulated in the liver, and effectively promoted the internalization of CBD in liver cells, thus effectively reducing the H2O2-induced oxidative damage of HepG2 cells and avoiding apoptosis. More importantly, CBD NPs effectively prevented CCl4-induced ALI by protecting liver function, ameliorating oxidative stress levels, inhibiting the production of inflammatory factors, and protecting the liver from histological damage. This study provides a promising strategy for achieving targeted delivery of CBD NPs in the liver, thereby effectively preventing ALI. [ABSTRACT FROM AUTHOR]

BACKGROUND: Cognitive impairment (CI) is a significant public health concern, and bioactive peptides have shown potential as therapeutic agents. However, information about their synergistic effects on cognitive function is still limited. Here, we investigated the synergistic effects of tilapia head protein hydrolysate (THPH) and walnut protein hydrolysate (WPH) in mitigating CI induced by scopolamine in mice. RESULTS: The results showed that the combined supplementation of THPH and WPH (mass ratio, 1:1) was superior to either individual supplement in enhancing spatial memory and object recognition abilities in CI mice, and significantly lessened brain injury in CI mice by alleviating neuronal damage, reducing oxidative stress and stabilizing the cholinergic system. In addition, the combined supplementation was found to be more conducive to remodeling the gut microbiota structure in CI mice by not only remarkably reducing the ratio of Firmicutes to Bacteroidota, but also specifically enriching the genus Roseburia. On the other hand, the combined supplementation regulated the disorders of sphingolipid and amino acid metabolism in CI mice, particularly upregulating glutathione and histidine metabolism, and displayed a stronger ability to increase the expression of genes and proteins related to the brain‐derived neurotrophic factor (BDNF)/TrkB/CrEB signaling pathway in the brain. CONCLUSION: These findings demonstrate that tilapia head and walnut‐derived protein hydrolysates exerted synergistic effects in ameliorating CI, which was achieved through modulation of gut microbiota, serum metabolic pathways and BDNF signaling pathways. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

The aim of this experiment was to investigate the ameliorative effect and molecular mechanism of tilapia head glycolipid (TH-GL) on indomethacin (IDM)-induced gastric ulcer in male Sprague Dawley (SD) rats. The gastric ulcer model was established by oral administration of 30 mg kg−1 IDM after 7 days of TH-GL or omeprazole (OME) administration in rats. Then the macroscopic gastric injury symptoms, gastric mucosa protective factor cyclooxygenase 1 (COX-1), cyclooxygenase 2 (COX-2), prostaglandin E2 (PGE2), the levels of oxidative stress, and inflammatory cytokine expression levels in the rats were analyzed. The experimental results showed that multiple ulcers appeared on the gastric surface of the rats in the model group. Compared to the model group, TH-GL significantly alleviated gastric ulcers and reduced the gastric damage index in rats. In addition, TH-GL significantly promoted the expression of constitutive enzyme COX-1 while inhibited the expression of inducible enzyme COX-2, and make PGE2 maintain at normal levels. TH-GL also inhibited oxidative stress and inflammatory responses, increased superoxide dismutase (SOD) activity and glutathione (GSH) content, decreased the level of malondialdehyde (MDA) and the content of pro-inflammatory factor. In conclusion, these results suggested that TH-GL could maintain the expression levels of COX-1 and PGE2 while inhibit the expression of COX-2 in the gastric of rat and then prevent IDM-induced gastric ulcer, which may be related to the regulation of oxidative stress and inflammatory response. Therefore, TH-GL might be a new option for the prevention of gastric diseases induced by IDM. [ABSTRACT FROM AUTHOR]