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43 results on '"Shen, Xiaoye"'

11. Evaluating Chlorine Sanitization at Practical Concentrations for Controlling Listeria monocytogenes and Salmonella on Fresh Peaches.

Author

Shen, Xiaoye, Hang, Mengqian, Su, Yuan, de Avila, Jeanene Marie, and Zhu, Mei-Jun

Subjects
STONE fruit, PRODUCE trade, DRINKING water, FRUIT industry, SALMONELLA, LISTERIA monocytogenes
Abstract

Recent foodborne outbreaks and recalls involving Listeria monocytogenes and Salmonella-contaminated peaches have caused significant economic losses to the peach industry. This study evaluated the effectiveness of chlorine, a commonly used sanitizer in the fresh produce industry, against L. monocytogenes and Salmonella and its ability to control cross-contamination in fresh peaches. Peaches inoculated with L. monocytogenes or Salmonella (~6 log10 CFU/peach) were treated with 50–150 mg/L of free chlorine (FC, pH6.8) 24 h post-inoculation. The results revealed that chlorine had similar efficacy against L. monocytogenes and Salmonella on peaches (p > 0.05). A 30 s treatment at 50, 100, and 150 mg/L FC resulted in dose-dependent reductions (p < 0.05), achieving reductions of 0.88–0.92, 1.54–1.61, and 1.73–1.79 log10 CFU/peach, respectively. Extending the contact time to 2 min slightly but significantly enhanced the chlorine efficacy (p < 0.05). Additionally, a 30 s to 2 min exposure to chlorine with 50–150 mg/L FC resulted in a 1.05–1.43 log10 CFU/peach reduction in yeasts and molds. Tap water exposure led to substantial cross-contamination between inoculated and uninoculated fruits and processed water, with Salmonella exhibiting higher transfer rates than L. monocytogenes. The application of chlorine mitigated the cross-contamination of both pathogens but did not entirely prevent it. These findings offer valuable insights for the peach and other stone fruit industries to verify process controls. [ABSTRACT FROM AUTHOR]

Published
2024
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18. Automatic system design for flange surface quality inspection with a bionic motion-vision paradigm

Author

Liang Gong, Jiaqi Zhang, Chengliang Liu, Xudong Li, Qin Zhengwei, Yuan Wei, and Shen Xiaoye

Subjects
Surface (mathematics), Computer science, media_common.quotation_subject, Mechanical strength, General Engineering, Mechanical engineering, Systems design, Motion vision, Quality (business), Flange, Joint (geology), Device failure, media_common
Abstract

Flanges are used to make connections. Their surface quality determines the joint mechanical strength and characteristics, while their defects lead to device failure. Complex surfaces and surface fl...

Published
2020
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26. Verification of peroxyacetic acid treatment against L. monocytogenes on fresh apples using E. faecium NRRL B-2354 as a surrogate in commercial spray-bar operations

Author

Shen, Xiaoye, primary, Su, Yuan, additional, Hua, Zi, additional, Cong, Jian, additional, Dhowlaghar, Nitin, additional, Sun, Qi, additional, Lin, Shengnan, additional, Green, Tonia, additional, Perrault, Mackenzie, additional, Galeni, Marcella, additional, Hanrahan, Ines, additional, Suslow, Trevor V., additional, and Zhu, Mei-Jun, additional

Published
2020
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36. Transcriptional profiling of Medicago truncatula under salt stress identified a novel CBF transcription factor MtCBF4 that plays an important role in abiotic stress responses

Author

Su Zhen, Ma Lei, Shen Xiaoye, Hu Xiaona, Zhang Yunqin, Li Daofeng, Wang Tao, and Dong Jiangli

Subjects
Botany, QK1-989
Abstract

Abstract Background Salt stress hinders the growth of plants and reduces crop production worldwide. However, different plant species might possess different adaptive mechanisms to mitigate salt stress. We conducted a detailed pathway analysis of transcriptional dynamics in the roots of Medicago truncatula seedlings under salt stress and selected a transcription factor gene, MtCBF4, for experimental validation. Results A microarray experiment was conducted using root samples collected 6, 24, and 48 h after application of 180 mM NaCl. Analysis of 11 statistically significant expression profiles revealed different behaviors between primary and secondary metabolism pathways in response to external stress. Secondary metabolism that helps to maintain osmotic balance was induced. One of the highly induced transcription factor genes was successfully cloned, and was named MtCBF4. Phylogenetic analysis revealed that MtCBF4, which belongs to the AP2-EREBP transcription factor family, is a novel member of the CBF transcription factor in M. truncatula. MtCBF4 is shown to be a nuclear-localized protein. Expression of MtCBF4 in M. truncatula was induced by most of the abiotic stresses, including salt, drought, cold, and abscisic acid, suggesting crosstalk between these abiotic stresses. Transgenic Arabidopsis over-expressing MtCBF4 enhanced tolerance to drought and salt stress, and activated expression of downstream genes that contain DRE elements. Over-expression of MtCBF4 in M. truncatula also enhanced salt tolerance and induced expression level of corresponding downstream genes. Conclusion Comprehensive transcriptomic analysis revealed complex mechanisms exist in plants in response to salt stress. The novel transcription factor gene MtCBF4 identified here played an important role in response to abiotic stresses, indicating that it might be a good candidate gene for genetic improvement to produce stress-tolerant plants.

Published
2011
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