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656 results on '"Shen, Xiaofeng"'

16. Feature Recognition Technology of Distribution Variable Voiceprint Based on the Principle of Multi-dimensional Space Phase Orbit Diagram

Author

Liang, Sheng, Yang, Bingfang, Shen, Xiaofeng, Wu, Jijian, Zhu, Kai, Zheng, Zhen, Wang, Zhaofan, Angrisani, Leopoldo, Series Editor, Arteaga, Marco, Series Editor, Panigrahi, Bijaya Ketan, Series Editor, Chakraborty, Samarjit, Series Editor, Chen, Jiming, Series Editor, Chen, Shanben, Series Editor, Chen, Tan Kay, Series Editor, Dillmann, Rüdiger, Series Editor, Duan, Haibin, Series Editor, Ferrari, Gianluigi, Series Editor, Ferre, Manuel, Series Editor, Hirche, Sandra, Series Editor, Jabbari, Faryar, Series Editor, Jia, Limin, Series Editor, Kacprzyk, Janusz, Series Editor, Khamis, Alaa, Series Editor, Kroeger, Torsten, Series Editor, Li, Yong, Series Editor, Liang, Qilian, Series Editor, Martín, Ferran, Series Editor, Ming, Tan Cher, Series Editor, Minker, Wolfgang, Series Editor, Misra, Pradeep, Series Editor, Möller, Sebastian, Series Editor, Mukhopadhyay, Subhas, Series Editor, Ning, Cun-Zheng, Series Editor, Nishida, Toyoaki, Series Editor, Oneto, Luca, Series Editor, Pascucci, Federica, Series Editor, Qin, Yong, Series Editor, Seng, Gan Woon, Series Editor, Speidel, Joachim, Series Editor, Veiga, Germano, Series Editor, Wu, Haitao, Series Editor, Zamboni, Walter, Series Editor, Zhang, Junjie James, Series Editor, Li, Jian, editor, Xie, Kaigui, editor, Hu, Jianlin, editor, and Yang, Qingxin, editor

Published
2023
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26. Allele-aware chromosome-level genome assembly of Artemisia annua reveals the correlation between ADS expansion and artemisinin yield

Author

Liao, Baosheng, Shen, Xiaofeng, Xiang, Li, Guo, Shuai, Chen, Shiyu, Meng, Ying, Liang, Yu, Ding, Dandan, Bai, Junqi, Zhang, Dong, Czechowski, Tomasz, Li, Yi, Yao, Hui, Ma, Tingyu, Howard, Caroline, Sun, Chao, Liu, Haitao, Liu, Jiushi, Pei, Jin, Gao, Jihai, Wang, Jigang, Qiu, Xiaohui, Huang, Zhihai, Li, Hongyi, Yuan, Ling, Wei, Jianhe, Graham, Ian, Xu, Jiang, Zhang, Boli, and Chen, Shilin

Published
2022
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29. Novel Differentially Expressed LncRNAs Regulate Artemisinin Biosynthesis in Artemisia annua.

Author

Ma, Tingyu, Zhang, Tianyuan, Song, Jingyuan, Shen, Xiaofeng, Xiang, Li, and Shi, Yuhua

Subjects
RNA sequencing, LINCRNA, SUSTAINABILITY, ARTEMISIA annua, ARTEMISININ
Abstract

Long non-coding RNAs (lncRNAs) are crucial in regulating secondary metabolite production in plants, but their role in artemisinin (ART) biosynthesis, a key anti-malarial compound from Artemisia annua, remains unclear. Here, by investigating high-artemisinin-producing (HAP) and lowartemisinin-producing (LAP) genotypes, we found that the final artemisinin content in A. annua is influenced by the quantity of the precursor compounds. We report on RNA deep sequencing in HAP and LAP genotypes. Based on the application of a stringent pipeline, 1419 novel lncRNAs were identified. Moreover, we identified 256 differentially expressed lncRNAs between HAP and LAP. We then established correlations between lncRNAs and artemisinin biosynthesis genes in order to identify a molecular framework for the differential expression of the pathway between the two genotypes. Three potential lncRNAs (MSTRG.33718.2, MSTRG.30396.1 and MSTRG.2697.4) linked to the key artemisinin biosynthetic genes (ADS: Amorpha-4,11-diene synthase, DXS: 1-deoxy-D-xylulose-5-phosphate synthase, and HMGS: 3-hydroxyl-3-methyglutaryl CoA synthase) were detected. Importantly, we observed that up-regulation of these lncRNAs positively modulates the target artemisinin biosynthetic genes, potentially leading to high artemisinin biosynthesis in HAP. In contrast, BAS (beta-amyrin synthase), which is involved in the artemisinin competing pathway, was strongly down-regulated in HAP compared to LAP, in line with the expression pattern of the linked lncRNA MSTRG.30396.1. By identifying and characterizing lncRNAs that are potentially linked to the regulation of key biosynthetic genes, this work provides new insights into the complex regulatory networks governing artemisinin production in A. annua. Such findings could pave the way for innovative approaches in metabolic engineering, potentially enhancing artemisinin yields and addressing challenges in sustainable production. [ABSTRACT FROM AUTHOR]

Published
2024
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30. Single-cell transcriptome profiling reveals the spatiotemporal distribution of triterpenoid saponin biosynthesis and transposable element activity in Gynostemma pentaphyllum shoot apexes and leaves

Author

Li, Rucan, primary, Du, Ke, additional, Zhang, Chuyi, additional, Shen, Xiaofeng, additional, Yun, Lingling, additional, Wang, Shu, additional, Li, Ziqin, additional, Sun, Zhiying, additional, Wei, Jianhe, additional, Li, Ying, additional, Guo, Baolin, additional, and Sun, Chao, additional

Published
2024
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38. ScRNA‐seq reveals the spatiotemporal distribution of camptothecin pathway and transposon activity in Camptotheca acuminata shoot apexes and leaves.

Author

Wang, Shu, Zhang, Chuyi, Li, Ying, Li, Rucan, Du, Ke, Sun, Chao, Shen, Xiaofeng, and Guo, Baolin

Subjects
GENE expression, SHOOT apexes, INDOLE alkaloids, CATHARANTHUS roseus, CYTOCHROME P-450
Abstract

Camptotheca acuminata Decne., a significant natural source of the anticancer drug camptothecin (CPT), synthesizes CPT through the monoterpene indole alkaloid (MIA) pathway. In this study, we used single‐cell RNA sequencing (scRNA‐seq) to generate datasets encompassing over 60,000 cells from C. acuminata shoot apexes and leaves. After cell clustering and annotation, we identified five major cell types in shoot apexes and four in leaves. Analysis of MIA pathway gene expression revealed that most of them exhibited heightened expression in proliferating cells (PCs) and vascular cells (VCs). In contrast to MIA biosynthesis in Catharanthus roseus, CPT biosynthesis in C. acuminata did not exhibit multicellular compartmentalization. Some putative genes encoding enzymes and transcription factors (TFs) related to the biosynthesis of CPT and its derivatives were identified through co‐expression analysis. These include 19 cytochrome P450 genes, 8 O‐methyltransferase (OMT) genes, and 62 TFs. Additionally, these pathway genes exhibited dynamic expression patterns during VC and EC development. Furthermore, by integrating gene and transposable element (TE) expression data, we constructed novel single‐cell transcriptome atlases for C. acuminata. This approach significantly facilitated the identification of rare cell types, including peripheral zone cells (PZs). Some TE families displayed cell type specific, tissue specific, or developmental stage‐specific expression patterns, suggesting crucial roles for these TEs in cell differentiation and development. Overall, this study not only provides novel insights into CPT biosynthesis and spatial–temporal TE expression characteristics in C. acuminata, but also serves as a valuable resource for further comprehensive investigations into the development and physiology of this species. [ABSTRACT FROM AUTHOR]

Published
2024
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