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453 results on '"Shen, Ren-Fang"'

25. OsHAK4 functions in retrieving sodium from the phloem at the reproductive stage of rice.

Author

Che, Jing, Yamaji, Naoki, Wang, Shao Fei, Xia, Yue, Yang, Shun Ying, Su, Yan Hua, Shen, Ren Fang, and Ma, Jian Feng

Subjects
SOIL salinity, RICE, CELL membranes, PHLOEM, IMMUNOSTAINING
Abstract

SUMMARY: Soil salinity significantly limits rice productivity, but it is poorly understood how excess sodium (Na+) is delivered to the grains at the reproductive stage. Here, we functionally characterized OsHAK4, a member of the clade IV HAK/KUP/KT transporter subfamily in rice. OsHAK4 was localized to the plasma membrane and exhibited influx transport activity for Na+, but not for K+. Analysis of organ‐ and growth stage‐dependent expression patterns showed that very low expression levels of OsHAK4 were detected at the vegetative growth stage, but its high expression in uppermost node I, peduncle, and rachis was found at the reproductive stage. Immunostaining indicated OsHAK4 localization in the phloem region of node I, peduncle, and rachis. Knockout of OsHAK4 did not affect the growth and Na+ accumulation at the vegetative stage. However, at the reproductive stage, the hak4 mutants accumulated higher Na+ in the peduncle, rachis, husk, and brown rice compared to the wild‐type rice. Element imaging revealed higher Na+ accumulation at the phloem region of the peduncle in the mutants. These results indicate that OsHAK4 plays a crucial role in retrieving Na+ from the phloem in the upper nodes, peduncle, and rachis, thereby preventing Na+ distribution to the grains at the reproductive stage of rice. [ABSTRACT FROM AUTHOR]

Published
2024
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26. Melatonin Increases Root Cell Wall Phosphorus Reutilization via an NO Dependent Pathway in Rice (Oryza sativa).

Author

Gao, Yong Qiang, Guo, Rui, Wang, Hao Yu, Sun, Jie Ya, Chen, Chang Zhao, Hu, Die, Zhong, Chong Wei, Jiang, Meng Meng, Shen, Ren Fang, Zhu, Xiao Fang, and Huang, Jiu

Subjects
RICE, GENE expression, AGRICULTURE, INTERNAL auditing, NITRIC oxide, RICE quality
Abstract

Melatonin (MT) has been implicated in the plant response to phosphorus (P) stress; however, the precise molecular mechanisms involved remain unclear. This study investigated whether MT controls internal P distribution and root cell wall P remobilization in rice. Rice was treated with varying MT and P levels and analyzed using biochemical and molecular techniques to study phosphorus utilization. The results demonstrated that low P levels lead to a rapid increase in endogenous MT levels in rice roots. Furthermore, the exogenous application of MT significantly improved rice tolerance to P deficiency, as evidenced by the increased biomass and reduced proportion of roots to shoots under P‐deficient conditions. MT application also mitigated the decrease in P content regardless in both the roots and shoots. Mechanistically, MT accelerated the reutilization of P, particularly in the root pectin fraction, leading to increased soluble P liberation. In addition, MT enhanced the expression of OsPT8, a gene involved in root‐to‐shoot P translocation. Furthermore, we observed that MT induced the production of nitric oxide (NO) in P‐deficient rice roots and that the mitigating effect of MT on P deficiency was compromised in the presence of the NO inhibitor, c‐PTIO, implying that NO is involved in the MT‐facilitated mitigation of P deficiency in rice. Overall, our findings highlight the potential of MT as a promising strategy for enhancing rice tolerance to P deficiency and improving P use efficiency in agricultural practices. [ABSTRACT FROM AUTHOR]

Published
2024
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