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Introduction: Platelet transfusion is a standard treatment to prevent bleeding in patients with hematological malignancies. Although transfusions can improve platelet count, their impact on platelet function remains controversial. Methods: We conducted flow cytometry to assess platelet function before and after transfusion and performed subgroup analyses to examine differences based on blood type, corrected count increment (CCI), and platelet microparticles. Results: Overall, 50 patients who received prophylactic platelet transfusion were enrolled. CD42b expression increased, whereas CD41 expression decreased after transfusion. Apheresis platelets exhibited the lowest expression of PAC‐1 and P‐selectin when exposed to agonist stimulations. PAC‐1 expression increased under high adenosine diphosphate (ADP) stimulation, while P‐selectin expression increased under both high ADP and thrombin receptor‐activating peptide stimulation. In the subgroup analysis, patients with a CCI >4500 and those with the same blood types exhibited a more significant increase in PAC‐1 and P‐selectin expression under agonist stimulation. When comparing apheresis platelets collected on different days, only the percentage of platelet‐derived microparticles showed a significant increase. Conclusion: Prophylactic transfusion improved platelet function. Platelet function significantly improved in patients with a CCI >4500, those with the same blood types as that of apheresis platelets, or those with platelet‐derived microparticle levels <4.7%. No significant improvement in platelet function was noted after the transfusion of different blood types with acceptable compatibility or the transfusion of incompatible blood types. Our results suggest that transfusing platelets with the same blood type remains the optimal choice. [ABSTRACT FROM AUTHOR]

Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2) plays an important role in driving immortalization of EBV-infected B cells through regulating the expression of many viral and cellular genes. We report a structural study of the tumor suppressor BS69/ZMYND11 C-terminal region, comprised of tandem coiled-coil-MYND domains (BS69CC-MYND), in complex with an EBNA2 peptide containing a PXLXP motif. The coiled-coil domain of BS69 self-associates to bring two separate MYND domains in close proximity, thereby enhancing the BS69 MYND-EBNA2 interaction. ITC analysis of BS69CC-MYND with a C-terminal fragment of EBNA2 further suggests that the BS69CC-MYND homodimer synergistically binds to the two EBNA2 PXLXP motifs that are respectively located in the conserved regions CR7 and CR8. Furthermore, we showed that EBNA2 interacts with BS69 and down-regulates its expression at both mRNA and protein levels in EBV-infected B cells. Ectopic BS69CC-MYND is recruited to viral target promoters through interactions with EBNA2, inhibits EBNA2-mediated transcription activation, and impairs proliferation of lymphoblastoid cell lines (LCLs). Substitution of critical residues in the MYND domain impairs the BS69-EBNA2 interaction and abolishes the BS69 inhibition of the EBNA2-mediated transactivation and LCL proliferation. This study identifies the BS69 C-terminal domains as an inhibitor of EBNA2, which may have important implications in development of novel therapeutic strategies against EBV infection.

Platelets play an essential role in primary hemostasis through bleeding and thromboembolism. Thus, the diagnosis or evaluation of impaired hereditary, acquired, and drug-related platelet dysfunction has become imperative. The assessment of the platelet function is too complex for routine platelet function study. The major methods involved in platelet function study include platelet function analyzer testing, thromboelastography, thromboelastometry, light transmission aggregometry, and flow cytometry. The current review article focuses on the methods with flow cytometry for immunophenotyping of platelet and evaluating platelet function for platelet disorders, especially in patients with thrombocytopenia. According to the consensus published by the International Society on Thrombosis and Haemostasis, for inherited and acquired platelet disorders, the two major measures by which flow cytometry determines platelet function are glycoprotein IIb/IIIa/P-selectin (CD62p) expression and percentage of leukocyte–platelet aggregates. Using flow cytometry to determine platelet function has several advantages, including good sensitivity to low platelet counts, small blood volume required, and the nonnecessity of centrifugation. However, flow cytometry has still many limitations and challenges, with standardization for routine laboratory testing also proving difficult. Although flow cytometry is available for multipurpose and sensitive study of platelet functions at the same time, the challenging analysis gradually increases and needs to be addressed before reality. [ABSTRACT FROM AUTHOR]

This paper presents a half-bridge single-phase two-wire (1[phi]2W) photovoltaic (PV) inverter system that can perform both active power filtering and real power injection. In the proposed system, it needs only two active switches, reducing cost significantly. In addition, output current of the inverter can be controlled to prevent switches from exceeding their current ratings. Thus, power rating of the inverter can be effectively used and power quality can be improved. For controlling inverter output current, an amplitude-clamping algorithm and an amplitude-scaling algorithm are proposed, which can determine inverter current command without needs of complicated calculation. Simulations and experimental results have verified the feasibility of the proposed PV inverter system and the two current control algorithms. Index Terms--Active power filtering (APF), amplitude-clamping algorithm (ACA), amplitude-scaling algorithm (ASA), half-bridge inverter, photovoltaic (PV) power injection.

This paper proposes a single-phase two-wire inverter system for photovoltaic (PV) power injection and active power filtering (APF) with nonlinear inductor consideration. The proposed system can fully or partially perform APF, process PV power, eliminate harmonic currents, improve power factor, and take into account the nonlinear effect of its output filter inductor. In the system, even though only the utility current is sensed, both APF and maximum power point tracking features can be still achieved, reducing the number of current sensors and cost significantly. To prevent output current from exceeding switch ratings, inverter current is properly controlled through a current estimator and a defined limit circle. A self-learning algorithm is also proposed to determine nonlinear inductance, which can increase the accuracy of the estimated current. Simulations and experimental results have verified the feasibility of the proposed PV inverter system and the algorithm. Index Terms--Current estimator, nonlinear inductor consideration, photovoltaic (PV) power injection, self-learning algorithm, single-phase two-wire (1 [empty set] 2W) inverter system.

This paper presents design and implementation of a single-phase three-wire (1[empty set]3W) inverter with grid connection and active power filtering which is based on nonlinear programming and fast-zero-phase detection (FZPD) algorithm. The proposed inverter system can transmit photovoltaic power, compensate harmonic currents, supply reactive power, and balance power at source side even when the line voltages are highly distorted. Simulated and experimental results have verified the FZPD algorithm and demonstrated that when the voltage sources are highly distorted, the proposed inverter system can yield better power quality over that with conventional zero-crossing-detection algorithm. Index Terms--Active power filter, fast-zero-phase detection (FZPD), grid connection, inverter, nonlinear programming, photovoltaic arrays.

This paper presents analysis, design, and practical considerations for 500 W boost power factor correctors (PFCs). The boost PFCs with three active soft-switching cells, which are boost, forward and flyback, and a passive soft-switching cell are first discussed. The operating principle and performance characteristics of the PFCs are then presented in detail, from which the boost with a flyback soft-switching cell and that with a passive soft-switching cell are designed and implemented. Performance comparison among these PFCs is also presented. Hardware measurements are used to verify the theoretical discussion. The presented information can provide designers a good application reference.

This paper presents a single-phase three-wire (1[phi] 3W) grid-connection photovoltaic (PV) power inverter with a feature of partial active power filter (PAPF), which can not only deal with PV power but filter current harmonics and improve power factor. Once the processed power exceeds the switch ratings, the inverter can reduce its output reactive power and harmonic power, while still supplying the maximum real power generated by the PV arrays. In derivation of control laws, a limit circle is defined to confine the output power of the inverter. To determine the power that the inverter can process, instantaneous reactive power of a 1[phi] 3W system is defined and used to calculate reactive power, which can avoid complex detections of phase angle and magnitude of the fundamental component of a nonlinear load current. Simulation results and experimental measurements have verified the proposed algorithm and the feasibility of the inverter.