1. The I.3.2 developmental mutant has a single nucleotide deletion in the gene centromere identifier .
- Author
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Evans CJ, Bieser KL, Acevedo-Vasquez KS, Augustine EJ, Bowen S, Casarez VA, Feliciano VI, Glazier A, Guinan HR, Hallman R, Haugan E, Hehr LA, Hunnicutt SN, Leifer I, Mauger M, Mauger M, Melendez NY, Milshteyn L, Moore E, Nguyen SA, Phanphouvong SC, Pinal DM, Pope HM, Salinas MM, Shellin M, Small I, Yeoh NC, Yokomizo AMK, and Kagey JD
- Abstract
The mutation I.3.2 was previously identified in a FLP/FRT screen of chromosome 2R for conditional growth regulators. Here we report the phenotypic characterization and genetic mapping of I.3.2 by undergraduate students participating in Fly-CURE, a pedagogical program that teaches the science of genetics through a classroom research experience. We find that creation of I.3.2 cell clones in the developing eye-antennal imaginal disc causes a headless adult phenotype, suggestive of both autonomous and non-autonomous effects on cell growth or viability. We also identify the I.3.2 mutation as a loss-of-function allele of the gene centromere identifier ( cid ), which encodes centromere-specific histone H3 variant critical for chromosomal segregation., (Copyright: © 2022 by the authors.)
- Published
- 2022
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