214 results on '"Shellfish allergy"'
Search Results
2. Risk Factors for Severe Seafood Allergy Among Adults in an Urban City in Vietnam
- Author
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Trinh TH, Duong CN, Pham TH, Au HD, Tran LT, Nguyen CV, Nguyen HB, Tran NM, Phan QQ, Le TN, and Nguyen N
- Subjects
seafood allergy ,shellfish allergy ,fish allergy ,vietnam. ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Tu HK Trinh,1 Chi N Duong,2 Tu HT Pham,3 Hao DT Au,3 Loc T Tran,3 Cuong V Nguyen,3 Han BG Nguyen,3 Nguyen M Tran,3 Quy Q Phan,3 Thu NA Le,3 Nga Nguyen4 1Center for Molecular Biomedicine, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam; 2University of Massachusetts Lowell, Lowell, MA, USA; 3Faculty of Medicine, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam; 4Hasaki Clinic, Ho Chi Minh City, VietnamCorrespondence: Tu HK Trinh, Center for Molecular Biomedicine, University of Medicine and Pharmacy at Ho Chi Minh City, 217 Hong Bang Street, Ward 11, District 5, Ho Chi Minh City, Vietnam, Tel +84-906 927 123, Email kim.tu.vn@ump.edu.vnPurpose: Increasing seafood consumption is associated with more frequent reports of food allergy. Little is known about seafood allergy (SFA) among adults in Vietnam. We investigated the characteristics of individuals with SFA and the risk factors for severe SFA.Patients and methods: A cross-sectional, web-based survey was conducted among individuals aged ≥ 18 years from universities in Ho Chi Minh City (Vietnam) between December 2021 and July 2022. The survey was based on a structured, validated questionnaire related to FA. Strict definitions of “convincing allergy” were used. Multivariate analysis was used to estimate the risk factors for severe SFA after adjusting for covariates. Data were analyzed using JASP (v.0.16.3) and SPSS (v.22.0).Results: Totally, 1038 out of 2137 (48.57%) individuals completed the questionnaire, of whom 285 (27.46%) had reported SFA. Convincing SFA accounted for 20.13% (209/1038) of the cases, with convincing shellfish allergy being more common than fish allergy. Participants with comorbid shellfish and fish allergy had higher prevalence of atopic dermatitis, peanut/nut allergy, other food allergy, and cutaneous and upper airway symptoms compared to participants with shellfish allergy (p < 0.05). The spectrum of reactive seafood was diverse and characterized by local species. The age of symptom onset was most commonly during late childhood and adolescence, with most reactions persisting into adulthood. A history of anaphylaxis, comorbid peanut, and tree nut allergy, and ≥ 3 allergens were associated with severe SFA.Conclusion: Features of causative, coexisting seafood allergy, and risk factors for severe SFA were demonstrated, which can provide a reference for future studies.Keywords: seafood allergy, shellfish allergy, fish allergy, Vietnam
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- 2024
3. Fish and shellfish allergy: Presentation and management differences in the UK and US—analysis of 945 patients
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Alla Nakonechna, MD, PhD, Ard van Bergen, PhD, Ariharan Anantharachagan, MD, Dilani Arnold, MD, Nicole Johnston, BS, Kari Nadeau, MD, PhD, Krzysztof Rutkowski, MD, Sayantani B. Sindher, MD, Panida Sriaroon, MD, Iason Thomas, MD, Pavaladurai Vijayadurai, MD, Annette Wagner, MD, and Carla M. Davis, MD
- Subjects
Seafood allergy ,fish allergy ,shellfish allergy ,total IgE ,fish specific IgE ,shellfish specific IgE ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Background: Seafood allergy (SA), including allergy to shellfish (crustacean and mollusks) and fish, is among the 4 most common food allergies causing anaphylaxis, but there are limited data showing SA clinical management in different countries. Objective: We sought to characterize a large cohort of patients with fish and shellfish allergy and to facilitate standardization of future care for this increasingly common allergic disease. Methods: We performed a retrospective, observational, noninterventional study from 945 patients from 2015 to 2019 in 7 hospitals in the United States and the United Kingdom to evaluate SA. A chi-square test was used to detect differences in family history, medical history, and current symptoms between patients in 2 countries. Results: Underdiagnosed anaphylaxis in patients with SA was associated with underuse of epinephrine (adrenaline) autoinjectors in both countries. Oral food challenge was used only when skin or serologic test results were negative. Asthma and allergic rhinitis were more common in the US patients with SA, but eczema was more common in UK patients with SA (P < .001). Respiratory, gastrointestinal, and neurological symptoms were higher in UK patients with SA than in US patients with SA (P < .001). Conclusions: In international multicenter cohorts of patients with fish and shellfish allergy, there are opportunities for improvement in management. Physician identification of anaphylaxis, use of diagnostic oral food challenges, and anaphylaxis treatment with epinephrine are areas with significant knowledge gaps in need of improvement in the United Kingdom and the United States. There is an opportunity for the development of unified, standardized diagnostic protocols for SA with distribution for allergists and trainees.
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- 2024
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4. Comprehending the allergen repertoire of shrimp for precision molecular diagnosis of shrimp allergy.
- Author
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Wai, Christine, Leung, Nicki, Leung, Agnes, Ngai, Sai, Pacharn, Punchama, Yau, Yat, Rosa Duque, Jaime, Kwan, Mike, Jirapongsananuruk, Orathai, Chan, Wai, Chua, Gilbert, Lee, Qun, Piboonpocanun, Surapon, Ho, Po, Wong, Joshua, Li, Shanshan, Xu, Kary, Wong, Gary, Chu, Ka, Leung, Patrick, Vichyanond, Pakit, and Leung, Ting
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DBPCFC ,allergen profiling ,component-resolved diagnosis ,shellfish allergy ,tropomyosin ,Allergens ,Fatty Acid-Binding Proteins ,Food Hypersensitivity ,Humans ,Hypersensitivity ,Immunoglobulin E ,Tropomyosin ,Troponin C - Abstract
BACKGROUND: Clinical management of shrimp allergy is hampered by the lack of accurate tests. Molecular diagnosis has been shown to more accurately reflect the clinical reactivity but the full spectrum of shrimp allergens and their clinical relevance are yet to be established. We therefore sought to comprehend the allergen repertoire of shrimp, investigate and compare the sensitization pattern and diagnostic value of the allergens in allergic subjects of two distinct populations. METHODS: Sera were collected from 85 subjects with challenge-proven or doctor-diagnosed shrimp allergy in Hong Kong and Thailand. The IgE-binding proteins of Penaeus monodon were probed by Western blotting and identified by mass spectrometry. Recombinant shrimp allergens were synthesized and analyzed for IgE sensitization by ELISA. RESULTS: Ten IgE-binding proteins were identified, and a comprehensive panel of 11 recombinant shrimp allergens was generated. The major shrimp allergens among Hong Kong subjects were troponin C (Pen m 6) and glycogen phosphorylase (Pen m 14, 47.1%), tropomyosin (Pen m 1, 41.2%) and sarcoplasmic-calcium binding protein (Pen m 4, 35.3%), while those among Thai subjects were Pen m 1 (68.8%), Pen m 6 (50.0%) and fatty acid-binding protein (Pen m 13, 37.5%). Component-based tests yielded significantly higher area under curve values (0.77-0.96) than shrimp extract-IgE test (0.70-0.75). Yet the best component test differed between populations; Pen m 1-IgE test added diagnostic value only in the Thai cohort, whereas sensitizations to other components were better predictors of shrimp allergy in Hong Kong patients. CONCLUSION: Pen m 14 was identified as a novel shrimp allergen predictive of challenge outcome. Molecular diagnosis better predicts shrimp allergy than conventional tests, but the relevant component is population dependent.
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- 2022
5. Probiotics in Infancy and Childhood for Food Allergy Prevention and Treatment.
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Di Costanzo, Margherita, Vella, Adriana, Infantino, Claudia, Morini, Riccardo, Bruni, Simone, Esposito, Susanna, and Biasucci, Giacomo
- Abstract
Food allergy represents a failure of oral tolerance mechanisms to dietary antigens. Over the past few years, food allergies have become a growing public health problem worldwide. Gut microbiota is believed to have a significant impact on oral tolerance to food antigens and in initiation and maintenance of food allergies. Therefore, probiotics have also been proposed in this field as a possible strategy for modulating both the gut microbiota and the immune system. In recent years, results from preclinical and clinical studies suggest a promising role for probiotics in food allergy prevention and treatment. However, future studies are needed to better understand the mechanisms of action of probiotics in food allergies and to design comparable study protocols using specific probiotic strains, defined doses and exposure times, and longer follow-up periods. [ABSTRACT FROM AUTHOR]
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- 2024
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6. Commercial shellfish skin prick test extracts show critical variability in allergen repertoire.
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Ruethers, Thimo, Johnston, Elecia B., Karnaneedi, Shaymaviswanathan, Nie, Shuai, Nugraha, Roni, Taki, Aya C., Kamath, Sandip D., Williamson, Nicholas A., Mehr, Sam S., Campbell, Dianne E., and Lopata, Andreas L.
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SKIN tests , *SHELLFISH , *ALLERGENS , *ALLERGENIC extracts , *RESEARCH personnel - Abstract
This article discusses the variability in allergen repertoire among commercial shellfish skin prick test (SPT) extracts. The study found that widely used commercial SPT extracts for shellfish allergy diagnosis are generally not standardized, limiting the diagnostic value of the results. The researchers analyzed 11 commercial crustacean and five mollusc SPT extracts and found significant variability in protein content and allergen repertoire. The study concludes that standardization of allergen extracts is urgently needed to improve the accuracy and reliability of SPT for shellfish allergy diagnosis. [Extracted from the article]
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- 2023
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7. The Use of Iodophor-Impregnated Drapes in Patients With Iodine-Related Allergies: A Case Series and Review of the Literature
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Andrew Fraval, MD, FRACS, Nicolina Zappley, BA, and William J. Hozack, MD
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Iodine allergy ,Shellfish allergy ,Povidone iodine allergy ,Adhesive incise drape ,Hip arthroplasty ,Knee arthroplasty ,Orthopedic surgery ,RD701-811 - Abstract
Background: The use of iodophor-impregnated adhesive drapes have become almost universally incorporated into standard practice of arthroplasty draping technique. Iodine-related allergies in patients planned for joint replacement present a challenge in terms of the best course of action to minimize complications and optimize outcomes. Methods: This is a retrospective case series of patients that received an iodophor-impregnated drape as part of draping for a total hip or knee arthroplasty at a single orthopaedic-specific hospital with documented iodine-related allergies. From 2015 to 2023, 9816 total hip arthroplasty and total knee arthroplasty cases were reviewed, and 135 were documented to have an iodine-related allergy for a prevalence of 1.38%. Intraoperative and postoperative records were reviewed to screen for an allergic reaction or wound healing issues that may have been related to an adverse reaction to the use of the iodophor-impregnated drape. Results: Of the 135 patients, 43 had iodine listed as an allergy, 85 had shellfish, 20 had iodinated contrast media, and 3 had povidone iodine. Sixteen patients had a cluster of iodine-related allergies. There were no intraoperative reports of an allergic reaction to this drape. There were four superficial wound problems, none of which were documented to relate to an allergic dermatitis reaction, and none required further surgery. Conclusions: Patients reporting iodine-related allergies were present in 1.38% of patients undergoing hip or knee arthroplasty in our series. We encountered no allergic reactions or adverse outcomes that could be attributed to the use of iodiphor impregnated drapes in these patients.
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- 2023
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8. IgE Mediated Shellfish Allergy in Children—A Review.
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Giannetti, Arianna, Pession, Andrea, Bettini, Irene, Ricci, Giampaolo, Giannì, Giuliana, and Caffarelli, Carlo
- Abstract
Shellfish is a leading cause of food allergy and anaphylaxis worldwide. Recent advances in molecular characterization have led to a better understanding of the allergen profile. High sequence homology between shellfish species and between shellfish and house dust mites leads to a high serological cross-reactivity, which does not accurately correlate with clinical cross-reactions. Clinical manifestations are immediate and the predominance of perioral symptoms is a typical feature of shellfish allergy. Diagnosis, as for other food allergies, is based on SPTs and specific IgE, while the gold standard is DBPCFC. Cross-reactivity between shellfish is common and therefore, it is mandatory to avoid all shellfish. New immunotherapeutic strategies based on hypoallergens and other innovative approaches represent the new frontiers for desensitization. [ABSTRACT FROM AUTHOR]
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- 2023
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9. Probiotics in Infancy and Childhood for Food Allergy Prevention and Treatment
- Author
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Margherita Di Costanzo, Adriana Vella, Claudia Infantino, Riccardo Morini, Simone Bruni, Susanna Esposito, and Giacomo Biasucci
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oral tolerance ,gut microbiota ,gut dysbiosis ,cow’s milk allergy ,egg allergy ,shellfish allergy ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Food allergy represents a failure of oral tolerance mechanisms to dietary antigens. Over the past few years, food allergies have become a growing public health problem worldwide. Gut microbiota is believed to have a significant impact on oral tolerance to food antigens and in initiation and maintenance of food allergies. Therefore, probiotics have also been proposed in this field as a possible strategy for modulating both the gut microbiota and the immune system. In recent years, results from preclinical and clinical studies suggest a promising role for probiotics in food allergy prevention and treatment. However, future studies are needed to better understand the mechanisms of action of probiotics in food allergies and to design comparable study protocols using specific probiotic strains, defined doses and exposure times, and longer follow-up periods.
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- 2024
- Full Text
- View/download PDF
10. IgE Mediated Shellfish Allergy in Children—A Review
- Author
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Arianna Giannetti, Andrea Pession, Irene Bettini, Giampaolo Ricci, Giuliana Giannì, and Carlo Caffarelli
- Subjects
shellfish allergy ,crustaceans ,molluscs ,tropomyosin ,oral food challenge ,allergen-specific immunotherapy ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Shellfish is a leading cause of food allergy and anaphylaxis worldwide. Recent advances in molecular characterization have led to a better understanding of the allergen profile. High sequence homology between shellfish species and between shellfish and house dust mites leads to a high serological cross-reactivity, which does not accurately correlate with clinical cross-reactions. Clinical manifestations are immediate and the predominance of perioral symptoms is a typical feature of shellfish allergy. Diagnosis, as for other food allergies, is based on SPTs and specific IgE, while the gold standard is DBPCFC. Cross-reactivity between shellfish is common and therefore, it is mandatory to avoid all shellfish. New immunotherapeutic strategies based on hypoallergens and other innovative approaches represent the new frontiers for desensitization.
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- 2023
- Full Text
- View/download PDF
11. Evaluating pharmacists' knowledge, attitude, and practices toward amiodarone cross-reactivity with iodine: a cross-sectional pilot study.
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Alshehab, Nura Abdullah, Alhumaid, Munirah Abdullah, Alabdulwahed, Mohammed Abdulrazaq, and Almulhim, Abdulaziz Saleh
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AMIODARONE ,SHELLFISH ,IODINE ,PHARMACISTS ,FOOD allergy ,CROSS reactions (Immunology) ,CROSS-sectional method - Abstract
Introduction: Iodine is a vital mineral of the human body that acts by maintaining the health of the thyroid gland. Research has shown that iodine-rich food allergy (i.e., seafood allergy) is not caused by iodine itself; instead, it is caused by other proteins including tropomyosin and parvalbumin in shellfish and fish, respectively. Amiodarone is a commonly used antiarrhythmic agent containing a significant amount of iodine. Objective: This study's objective was to assess pharmacists' knowledge, attitude, and practices toward the misconception of iodine allergy and the cross-reactivity with amiodarone. Methods: In February 2020, a cross-sectional study was conducted by sending out an online survey to three pharmaceutical organizations (Saudi Pharmaceutical Society, Kuwait Pharmaceutical Association, and Oman Pharmaceutical Society). Additionally, an electronic questionnaire was administered to pharmacists attending the Dubai International Pharmaceuticals and Technologies Conference and Exhibition 2020 (DUPHAT). Chi-square or Fisher's exact test, when appropriate, were used to compare categorical variables. The statistical analyses were carried out using SPSS software. Results: Data were collected from 66 respondents. However, only 61 (92.4%) were included in the final analysis following the exclusion of incomplete responses. The mean age of participants was 35 ± 8.48 years. The majority of participants did not have the Board of Pharmacy Specialties Certification (54.1%). Moreover, (41%) of participants licensed as pharmacists with more than 10 years of initial pharmacy licensure. Forty-three (70.5%) of participants had the misconception that iodine allergy should be considered before amiodarone administration, 20/32 (62.5%) of whom considered iodine allergy alone thought that premedication with corticosteroids and/or antihistamines is necessary. Concerning iodine allergy and amiodarone use, there was no significant difference in knowledge between the pharmacists who have board certification and those who did not. Conclusion: Pharmacists' misconception concerning iodine allergy and cross-reactivity with amiodarone was evident. Implementation of educational programs targeting pharmacists is necessary to correct these misconceptions. [ABSTRACT FROM AUTHOR]
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- 2022
- Full Text
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12. In Silico Prediction of Cross-Reactive Epitopes of Tropomyosin from Shrimp and Other Arthropods Involved in Allergy.
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Saetang, Jirakrit, Tipmanee, Varomyalin, and Benjakul, Soottawat
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TROPOMYOSINS , *DERMATOPHAGOIDES pteronyssinus , *EPITOPES , *COCKROACHES , *HOUSE dust mites , *HISTOCOMPATIBILITY antigens , *ARTHROPODA , *T cells - Abstract
Tropomyosin in shellfish is considered a major cross-reactive allergen in house dust mites and cockroaches; however, the specific epitopes have not been elucidated. Therefore, this study aimed to identify the consensus antigenic determinant among shrimp, house dust mites, and cockroaches using in silico methods. The protein sequences of tropomyosin, including Der f 10, Mac r 1, Pen a 1, Pen m 1, Per a 7, and Bla g 7, were retrieved from the UniProt database. The 3D structures were derived from the AlphaFold or modeled using the Robetta. The determination of linear epitopes was performed by AlgPRED and BepiPRED for B cell epitope, and NetMHCIIpan and NetMHCII for T cell epitope, while Ellipro was used to evaluate conformational epitopes. Fourteen peptides were discovered as the consensus linear B cell epitopes, while seventeen peptides were identified as linear T cell epitopes specific to high-frequency HLA-DR and HLA-DQ alleles. The conformational determination of B cell epitopes provided nine peptides, in which residues 209, 212, 255–256, and 258–259 were found in both linear B cell and linear T cell epitope analysis. This data could be utilized for further in vitro study and may contribute to immunotherapy for allergic diseases associated with tropomyosin. [ABSTRACT FROM AUTHOR]
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- 2022
- Full Text
- View/download PDF
13. Evaluating pharmacists’ knowledge, attitude, and practices toward amiodarone cross-reactivity with iodine: a cross-sectional pilot study
- Author
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Nura Abdullah Alshehab, Munirah Abdullah Alhumaid, Mohammed Abdulrazaq Alabdulwahed, and Abdulaziz Saleh Almulhim
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Amiodarone ,Iodine allergy ,Shellfish allergy ,Radiocontrast media allergy ,Hypersensitivity ,Pharmacist ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
Introduction Iodine is a vital mineral of the human body that acts by maintaining the health of the thyroid gland. Research has shown that iodine-rich food allergy (i.e., seafood allergy) is not caused by iodine itself; instead, it is caused by other proteins including tropomyosin and parvalbumin in shellfish and fish, respectively. Amiodarone is a commonly used antiarrhythmic agent containing a significant amount of iodine. Objective This study’s objective was to assess pharmacists’ knowledge, attitude, and practices toward the misconception of iodine allergy and the cross-reactivity with amiodarone. Methods In February 2020, a cross-sectional study was conducted by sending out an online survey to three pharmaceutical organizations (Saudi Pharmaceutical Society, Kuwait Pharmaceutical Association, and Oman Pharmaceutical Society). Additionally, an electronic questionnaire was administered to pharmacists attending the Dubai International Pharmaceuticals and Technologies Conference and Exhibition 2020 (DUPHAT). Chi-square or Fisher’s exact test, when appropriate, were used to compare categorical variables. The statistical analyses were carried out using SPSS software. Results Data were collected from 66 respondents. However, only 61 (92.4%) were included in the final analysis following the exclusion of incomplete responses. The mean age of participants was 35 ± 8.48 years. The majority of participants did not have the Board of Pharmacy Specialties Certification (54.1%). Moreover, (41%) of participants licensed as pharmacists with more than 10 years of initial pharmacy licensure. Forty-three (70.5%) of participants had the misconception that iodine allergy should be considered before amiodarone administration, 20/32 (62.5%) of whom considered iodine allergy alone thought that premedication with corticosteroids and/or antihistamines is necessary. Concerning iodine allergy and amiodarone use, there was no significant difference in knowledge between the pharmacists who have board certification and those who did not. Conclusion Pharmacists’ misconception concerning iodine allergy and cross-reactivity with amiodarone was evident. Implementation of educational programs targeting pharmacists is necessary to correct these misconceptions.
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- 2022
- Full Text
- View/download PDF
14. Shelling the myth: allergies to Iodine containing substances and risk of reaction to Iodinated contrast media.
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Bruen, Richard, Stirling, Aaron, Ryan, M., Sheehan, M., and MacMahon, Peter
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CONTRAST media , *MEDICAL personnel , *IODINE deficiency , *ALLERGIES , *SCIENCE databases , *IODINE - Abstract
Purpose: In excess of 100 million procedures using iodinated radio-contrast media are conducted each year. There is a common misunderstanding regarding the links between allergy to iodinated substances and the risk of allergic reaction to intravenous iodinated contrast agents. These perceived risks are managed via administration of corticosteroids or avoidance of iodinated contrast altogether. Methods: An extensive review of published literature on scientific databases and international guidelines was conducted in order to inform the research question. A questionnaire was formulated and distributed to hospital doctors in four tertiary centres. Within this questionnaire, hospital doctors were presented with six different scenarios of bona fide allergy to iodinated substances (e.g. shellfish) and asked to select the treatment response option which they deemed to be the most suitable from a choice of three (standard contrast scan/delay scan with pre-medication/change to non-contrast scan). Results: Eighty-seven questionnaire responses were received. Contrast (standard protocol) was the most appropriate regimen in the setting of all the listed allergies. This was identified correctly by 76%, 69%, 44%, 32%, 18% and 14% for kiwi, fish, poly-food, shellfish, betadine and tincture of iodine allergies, respectively. Conclusions: There is a lack of understanding amongst local junior medical staff regarding administration of iodinated contrast media to patients with a history of allergy to iodinated substances. These misconceptions may potentiate the unnecessary usage of pre-medication and ordering of non-contrast scans in the setting of a gold-standard enhanced scan. Findings from this study suggest that there is a need for future education efforts targeted during the basic specialty training stage. [ABSTRACT FROM AUTHOR]
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- 2022
- Full Text
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15. Shrimp‐allergic patients in a multi‐food oral immunotherapy trial.
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Nguyen, Diem‐Tran I., Sindher, Sayantani B., Chinthrajah, R. Sharon, Nadeau, Kari, Davis, Carla M., and Ebisawa, Motohiro
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ALLERGIC conjunctivitis , *PEANUT allergy , *FOOD allergy , *IMMUNOTHERAPY , *WHITELEG shrimp - Abstract
Anaphylaxis, desensitization, food allergen, food allergy, omalizumab, oral immunotherapy, shellfish allergy, shrimp allergy, sustained unresponsiveness In addition, commercial shrimp extracts are heterogeneous with variable allergen representation.11 The differing allergens may result in varied responses in SPT and IgE results, which may explain Patient B's apparent negative shrimp IgE test. Keywords: anaphylaxis; desensitization; food allergen; food allergy; omalizumab; oral immunotherapy; shellfish allergy; shrimp allergy; sustained unresponsiveness EN anaphylaxis desensitization food allergen food allergy omalizumab oral immunotherapy shellfish allergy shrimp allergy sustained unresponsiveness 1 4 4 01/05/22 20220101 NES 220101 AUTHOR CONTRIBUTIONS B Diem-Tran Nguyen: b Data curation (equal); Investigation (equal); Project administration (lead); Visualization (lead); Writing-original draft (lead); Writing-review & editing (equal). [Extracted from the article]
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- 2022
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16. Fish and shellfish allergy: Presentation and management differences in the UK and US-analysis of 945 patients.
- Author
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Nakonechna A, van Bergen A, Anantharachagan A, Arnold D, Johnston N, Nadeau K, Rutkowski K, Sindher SB, Sriaroon P, Thomas I, Vijayadurai P, Wagner A, and Davis CM
- Abstract
Background: Seafood allergy (SA), including allergy to shellfish (crustacean and mollusks) and fish, is among the 4 most common food allergies causing anaphylaxis, but there are limited data showing SA clinical management in different countries., Objective: We sought to characterize a large cohort of patients with fish and shellfish allergy and to facilitate standardization of future care for this increasingly common allergic disease., Methods: We performed a retrospective, observational, noninterventional study from 945 patients from 2015 to 2019 in 7 hospitals in the United States and the United Kingdom to evaluate SA. A chi-square test was used to detect differences in family history, medical history, and current symptoms between patients in 2 countries., Results: Underdiagnosed anaphylaxis in patients with SA was associated with underuse of epinephrine (adrenaline) autoinjectors in both countries. Oral food challenge was used only when skin or serologic test results were negative. Asthma and allergic rhinitis were more common in the US patients with SA, but eczema was more common in UK patients with SA ( P < .001). Respiratory, gastrointestinal, and neurological symptoms were higher in UK patients with SA than in US patients with SA ( P < .001)., Conclusions: In international multicenter cohorts of patients with fish and shellfish allergy, there are opportunities for improvement in management. Physician identification of anaphylaxis, use of diagnostic oral food challenges, and anaphylaxis treatment with epinephrine are areas with significant knowledge gaps in need of improvement in the United Kingdom and the United States. There is an opportunity for the development of unified, standardized diagnostic protocols for SA with distribution for allergists and trainees., Competing Interests: Disclosure of potential conflict of interest: K. Nadeau reported receiving grants from the 10.13039/100000060National Institute of Allergy and Infectious Diseases, the National Heart, Lung, and Blood Institute, the 10.13039/100000066National Institute of Environmental Health Sciences, and Food Allergy Research and Education; serving as director of the World Allergy Organization; serving as advisor for Cour Pharma; serving on the National Scientific Committee of the 10.13039/100014247Immune Tolerance Network and the National Institutes of Health clinical research centers; being cofounder of Before Brands, Latitude, Alladapt, and IgGenix outside the submitted work; and having patents for an oral formula for decreasing food allergy risk and treatment for food allergy, for granulocyte-based methods for detecting and monitoring immune system disorders issued, for methods and assays for detecting and quantifying pure subpopulations of white blood cells in immune system disorders, and for microfluidic device and diagnostic methods for allergy testing based on detection of basophil activation pending. S. B. Sindher is funded by the National Institute of Allergy and Infectious Disease, Consortium for Food Allergy Research (CoFAR), Regeneron, DBV Technologies, Aimmune Therapeutics, Novartis, and Sanofi, and is an advisory member for AstraZeneca. P. Sriaroon is funded by Food Allergy Research and Education. C. M. Davis receives research contract funding from DBV Technologies, Regeneron, and Aimmune Therapeutics, grant funding from the National Institute of Allergy and Infectious Disease (grant no. R34AI157948) (CoFAR and Consortium of Eosinophilic Gastrointestinal Disease Researchers), and the Food Allergy Research and Education. The rest of the authors declare that they have no relevant conflicts of interest., (© 2024 The Author(s).)
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- 2024
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17. In Silico Prediction of Cross-Reactive Epitopes of Tropomyosin from Shrimp and Other Arthropods Involved in Allergy
- Author
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Jirakrit Saetang, Varomyalin Tipmanee, and Soottawat Benjakul
- Subjects
shellfish allergy ,tropomyosin ,cross-reactivity ,house dust mite ,cockroach ,epitope ,Organic chemistry ,QD241-441 - Abstract
Tropomyosin in shellfish is considered a major cross-reactive allergen in house dust mites and cockroaches; however, the specific epitopes have not been elucidated. Therefore, this study aimed to identify the consensus antigenic determinant among shrimp, house dust mites, and cockroaches using in silico methods. The protein sequences of tropomyosin, including Der f 10, Mac r 1, Pen a 1, Pen m 1, Per a 7, and Bla g 7, were retrieved from the UniProt database. The 3D structures were derived from the AlphaFold or modeled using the Robetta. The determination of linear epitopes was performed by AlgPRED and BepiPRED for B cell epitope, and NetMHCIIpan and NetMHCII for T cell epitope, while Ellipro was used to evaluate conformational epitopes. Fourteen peptides were discovered as the consensus linear B cell epitopes, while seventeen peptides were identified as linear T cell epitopes specific to high-frequency HLA-DR and HLA-DQ alleles. The conformational determination of B cell epitopes provided nine peptides, in which residues 209, 212, 255–256, and 258–259 were found in both linear B cell and linear T cell epitope analysis. This data could be utilized for further in vitro study and may contribute to immunotherapy for allergic diseases associated with tropomyosin.
- Published
- 2022
- Full Text
- View/download PDF
18. Usefulness of the Nasal Allergen Provocation Test in the Diagnosis of Shellfish Allergy
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A Valero, Mariona Pascal, Enrique Fernández-Caldas, G Araujo-Sánchez, Joan Bartra, E Abel Fernández, M Rueda, and S Gelis
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medicine.medical_specialty ,Nasal Provocation Tests ,animal structures ,medicine.medical_treatment ,Immunology ,Provocation test ,Shellfish Hypersensitivity ,medicine.disease_cause ,Nasal provocation test ,Allergen ,Acoustic rhinometry ,Food allergy ,medicine ,Humans ,Immunology and Allergy ,Anaphylaxis ,Skin Tests ,Plant Extracts ,Oral food challenge ,business.industry ,fungi ,Shellfish allergy ,Allergens ,Immunoglobulin E ,medicine.disease ,Dermatology ,Shrimp ,business - Abstract
Background: Shellfish allergy is a major cause of food allergy and anaphylaxis worldwide. Several allergenic proteins have been described in the last few years, but the only diagnostic tool that still enables discrimination between allergic and nonallergic sensitized persons is the oral food challenge (OFC). Objective: The aim of this study was to evaluate the usefulness of the nasal allergen provocation test (NAPT) as a diagnostic tool in shellfish allergy. Methods: Forty-five patients with confirmed sensitization to shrimp by a positive skin prick test (SPT) result with a commercial shrimp extract were recruited and classified as sensitized-allergic or sensitized-nonallergic based on current tolerance to shrimp intake, the result of an OFC with a freeze-dried cooked shrimp mixture extract, or a recent history of anaphylaxis induced by shrimp ingestion. These patients and 10 controls not sensitized to shrimp underwent NAPT with a freeze-dried cooked shrimp mixture extract. The response was evaluated using acoustic rhinometry and a visual analog scale. Results: Significant differences (P=.001) were found between the sensitized-allergic group (18/20 positive NAPT, 90%) and both the sensitized-nonallergic group (2/18 positive NAPT, 11.1%) and controls (0/10 positive NAPT). NAPT enables differentiation between allergic and nonallergic persons with a sensitivity of 90%, specificity of 89%, positive predictive value of 90%, and negative predictive value of 89%. Conclusions: Our results indicate that NAPT makes it possible to differentiate between sensitized symptomatic patients and sensitized tolerant patients and could be a valuable diagnostic tool when assessing shrimp allergy.
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- 2022
19. Recombinant Tropomyosin from the Pacific Oyster (Crassostrea gigas) for Better Diagnosis
- Author
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Roni Nugraha, Thimo Ruethers, Aya C. Taki, Elecia B. Johnston, Shaymaviswanathan Karnaneedi, Sandip D. Kamath, and Andreas L. Lopata
- Subjects
Cra g 1 ,molecular allergology ,tropomyosin ,Pacific oyster ,mollusc allergen ,shellfish allergy ,Chemical technology ,TP1-1185 - Abstract
The Pacific oyster is a commercially important mollusc and, in contrast to most other shellfish species, frequently consumed without prior heat treatment. Oysters are rich in many nutrients but can also cause food allergy. Knowledge of their allergens and cross-reactivity remains very limited. These limitations make an optimal diagnosis of oyster allergy difficult, in particular to the Pacific oyster (Crassostrea gigas), the most cultivated and consumed oyster species worldwide. This study aimed to characterise IgE sensitisation profiles of 21 oyster-sensitised patients to raw and heated Pacific oyster extract using immunoblotting and advanced mass spectrometry, and to assess the relevance of recombinant oyster allergen for improved diagnosis. Tropomyosin was identified as the major allergen recognised by IgE from 18 of 21 oyster-sensitised patients and has been registered with the WHO/IUIS as the first oyster allergen (Cra g 1). The IgE-binding capacity of oyster-sensitised patients’ IgE to purified natural and recombinant tropomyosin from oyster, prawn, and dust mite was compared using enzyme-linked immunosorbent assay. The degree of IgE binding varied between patients, indicating partial cross-sensitisation and/or co-sensitisation. Amino acid sequence alignment of tropomyosin from these three species revealed five regions that contain predicted IgE-binding epitopes, which are most likely responsible for this cross-reactivity. This study fully biochemically characterises the first and major oyster allergen Cra g 1 and demonstrates that the corresponding recombinant tropomyosin should be implemented in improved component-resolved diagnostics and guide future immunotherapy.
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- 2022
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20. Linear Epitopes Play an Important Role in the Immunoglobulin G (IgG)/Immunoglobulin E (IgE)-Binding Capacity of Scy p 4
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Meng Liu, Meng-Si Li, Min-Jie Cao, Gui-Xia Chen, Guang-Ming Liu, Fei Huan, Qing-Mei Liu, Xiao Yun, Dong Lai, and Yi-Yu Chen
- Subjects
biology ,Shellfish allergy ,Scylla paramamosain ,Shellfish Hypersensitivity ,General Chemistry ,Allergens ,Immunoglobulin E ,medicine.disease ,biology.organism_classification ,medicine.disease_cause ,Molecular biology ,Epitope ,Immunoglobulin G ,Dithiothreitol ,Epitopes ,chemistry.chemical_compound ,Allergen ,chemistry ,biology.protein ,medicine ,Iodoacetamide ,Humans ,General Agricultural and Biological Sciences - Abstract
Sarcoplasmic calcium-binding protein is a stable allergen in Scylla paramamosain and named Scy p 4. To explore the importance of linear epitopes in the immunoglobulin G (IgG)/immunoglobulin E (IgE)-binding capacity of Scy p 4, chemical denaturants were used to destroy the structure. Scy p 4 was reduced with dithiothreitol and subsequently alkylated with iodoacetamide (IAA). Furthermore, the structural analysis indicated that IAA-Scy p 4 was an unstructured protein. The inhibition enzyme-linked immunosorbent assay showed that IAA-Scy p 4 could inhibit the binding of Scy p 4 to sensitize serum, with inhibition rates reached 55%. Moreover, the linear mimotopes of Scy p 4 were predicted in silico. Three linear epitopes were verified by serological tests and named L-Scy p 4-1 (AA76-91), L-Scy p 4-2 (AA111-125), and L-Scy p 4-3 (AA137-146). Overall, these data provide an understanding of the relationship between the structure and allergenicity about Scy p 4, and the identified linear epitopes can be used for diagnosis and food processing of shellfish allergy.
- Published
- 2021
21. Food-Induced Anaphylaxis
- Author
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Järvinen-Seppo, Kirsi M., Nowak-Węgrzyn, Anna, and Castells, Mariana C., editor
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- 2011
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22. The Use of Iodophor-Impregnated Drapes in Patients With Iodine-Related Allergies: A Case Series and Review of the Literature.
- Author
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Fraval A, Zappley N, and Hozack WJ
- Abstract
Background: The use of iodophor-impregnated adhesive drapes have become almost universally incorporated into standard practice of arthroplasty draping technique. Iodine-related allergies in patients planned for joint replacement present a challenge in terms of the best course of action to minimize complications and optimize outcomes., Methods: This is a retrospective case series of patients that received an iodophor-impregnated drape as part of draping for a total hip or knee arthroplasty at a single orthopaedic-specific hospital with documented iodine-related allergies. From 2015 to 2023, 9816 total hip arthroplasty and total knee arthroplasty cases were reviewed, and 135 were documented to have an iodine-related allergy for a prevalence of 1.38%. Intraoperative and postoperative records were reviewed to screen for an allergic reaction or wound healing issues that may have been related to an adverse reaction to the use of the iodophor-impregnated drape., Results: Of the 135 patients, 43 had iodine listed as an allergy, 85 had shellfish, 20 had iodinated contrast media, and 3 had povidone iodine. Sixteen patients had a cluster of iodine-related allergies. There were no intraoperative reports of an allergic reaction to this drape. There were four superficial wound problems, none of which were documented to relate to an allergic dermatitis reaction, and none required further surgery., Conclusions: Patients reporting iodine-related allergies were present in 1.38% of patients undergoing hip or knee arthroplasty in our series. We encountered no allergic reactions or adverse outcomes that could be attributed to the use of iodiphor impregnated drapes in these patients., (© 2023 The Authors.)
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- 2023
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23. Shellfish allergy–induced overlap chronic graft-versus-host disease
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Hayley Braun and Justin Cheeley
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Allergy ,BSA, body surface area ,Case Report ,Dermatology ,GVHD, graft-versus-host disease ,Acute graft versus host disease ,aGVHD, acute graft-versus-host disease ,graft-versus-host disease ,stem cell transplant ,Medicine ,Shellfish ,GVHD - Graft-versus-host disease ,Body surface area ,business.industry ,Shellfish allergy ,allergy ,medicine.disease ,shellfish ,Graft-versus-host disease ,cGVHD, chronic graft-versus-host disease ,BSA - Body surface area ,ocGVHD, overlap chronic graft-versus-host disease ,RL1-803 ,Immunology ,business ,SCT, stem cell transplantation - Published
- 2021
24. A ratiometric fluorescent aptasensor based on EXPAR to detect shellfish tropomyosin in food system.
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Zhou, Jinru, Wang, Yanbo, Zhou, Chaosheng, Zheng, Lei, and Fu, Linglin
- Subjects
- *
TROPOMYOSINS , *SHELLFISH , *ALLERGENS , *APTAMERS - Abstract
Shellfish allergy represents a crucial public health problem, resulting in an urgent requirement for an accurate, simple, and reliable method to quantify shellfish allergens in the complex food system. Tropomyosin (TM) is known as the major allergen in shellfish. Herein, a novel switch-conversional ratiometric fluorescent biosensor, using TM-specific aptamer as the recognition probe, was developed to detect TM in the complex food system. The proposed aptasensor exhibited good accuracy, stability, and selectivity for the quantification of TM in foodstuffs. Furthermore, the applicability of this proposed aptasensor in complex food samples was verified using seven different shellfish samples. Therefore, this ratiometric fluorescent aptasensor enables accurately detecting the presence of TM traces in foods, providing technical support for allergen label supervision. • A EXPAR based-ratiometric aptasensor was developed to detect shellfish allergen TM. • TM could achieve the highly specific and accurate detection. • Applicability of proposed sensor in food samples was validated. [ABSTRACT FROM AUTHOR]
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- 2023
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25. Food Protein-Induced Enterocolitis Syndrome
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Sam Mehr, M. Cecilia Berin, and Anna Nowak-Wegrzyn
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Pediatrics ,medicine.medical_specialty ,Population ,03 medical and health sciences ,Lethargy ,0302 clinical medicine ,Food allergy ,Humans ,Immunology and Allergy ,Medicine ,030212 general & internal medicine ,education ,Aged ,Enterocolitis ,education.field_of_study ,business.industry ,Oral food challenge ,Shellfish allergy ,Infant ,Syndrome ,Allergens ,Middle Aged ,medicine.disease ,Hypotonia ,Food protein-induced enterocolitis syndrome ,030228 respiratory system ,Dietary Proteins ,medicine.symptom ,business ,Food Hypersensitivity - Abstract
Food protein-induced enterocolitis syndrome (FPIES) is a non-IgE-mediated food allergy that manifests with projectile, repetitive emesis that can be followed by diarrhea and may be accompanied by lethargy, hypotonia, hypothermia, hypotension, and metabolic derangements. FPIES usually starts in infancy although onset at older ages is being increasingly recognized. FPIES is not rare, with the cumulative incidence of FPIES in infants estimated to be 0.015% to 0.7%, whereas the population prevalence in the US infants was 0.51%. FPIES diagnosis is challenging and might be missed because of later (1-4 hours) onset of symptoms after food ingestion, lack of typical allergic skin and respiratory symptoms, and food triggers that are perceived to be hypoallergenic. Diagnosis is based on the recognition of symptoms because there are no biomarkers of FPIES. The pathophysiology remains obscure although activation of the innate immune compartment has been detected. Management relies of avoidance of food triggers, treatment of accidental exposures, and periodic re-evaluations with supervised oral food challenges to monitor for resolution. There are no strategies to accelerate development of tolerance in FPIES. Here we review the most important current concepts in epidemiology, pathophysiology, diagnosis, and management of FPIES.
- Published
- 2020
26. Physicochemical characterization and identification of major linear epitopes of sarcoplasmic calcium-binding protein (SCP) allergen from Pacific oyster (Crassostrea gigas)
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Xiaohan Zhao, Qingli Cheng, Liu Wenying, Guoming Li, Ruizeng Gu, Xiao-Wen Feng, and Lu Jun
- Subjects
Oyster ,medicine.disease_cause ,Epitope ,law.invention ,Epitopes ,Allergen ,law ,biology.animal ,medicine ,Animals ,Amino Acid Sequence ,Crassostrea ,Peptide sequence ,Nutrition and Dietetics ,biology ,Calcium-Binding Proteins ,Shellfish allergy ,Pacific oyster ,Allergens ,medicine.disease ,biology.organism_classification ,Rats ,Biochemistry ,Recombinant DNA ,Agronomy and Crop Science ,Food Science ,Biotechnology - Abstract
BACKGROUND Food allergy has been regarded as a serious public nutritional health problem and attracted extensive attention all over the world, of which shellfish allergy is a long-lasting disorder that impacts health through a life-time. Sarcoplasmic calcium-binding protein (SCP) plays a vital role in cell and muscle functions and has been identified as an allergen in oyster. RESULTS In this study, recombinant SCP (rSCP) with a molecular mass of 21 kDa was produced and identified based on SCP amino acid sequence of pacific oyster (Crassostrea gigas), and was used as a follow-up experimental material. Its physicochemical characterization showed that the purified rSCP is highly stable to heat, acid-alkali and trypsin digestion, but weaker to pepsin digestion. We established an animal sensitization model and rSCP displayed stronger IgE-binding activity with rats serum in the rSCP+ CT group compared with the CT group and control group. Most importantly, five epitope peptides were identified as linear immunodominant epitopes by indirect competitive enzyme-linked immunosorbent assay (icELISA) for the first time. In addition, we also found that conformational epitopes may play a major role in immunoreactivity of SCP. CONCLUSION These results are significant for understanding hypersensitization of humans to oyster and offer available preventive measures and treatment programs in further research. This article is protected by copyright. All rights reserved.
- Published
- 2021
27. Shrimp‐allergic patients in a multi‐food oral immunotherapy trial
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R. Sharon Chinthrajah, Carla M. Davis, Kari C. Nadeau, Diem-Tran I Nguyen, and Sayantani B. Sindher
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Clinical Trials as Topic ,Oral immunotherapy ,business.industry ,medicine.medical_treatment ,Immunology ,Shellfish allergy ,Administration, Oral ,Omalizumab ,Allergens ,medicine.disease ,Shrimp ,Desensitization, Immunologic ,Food allergy ,Pediatrics, Perinatology and Child Health ,medicine ,Humans ,Immunology and Allergy ,Immunotherapy ,Food allergens ,business ,Food Hypersensitivity ,Anaphylaxis ,medicine.drug ,Desensitization (medicine) - Published
- 2021
28. Race/ethnicity, but not income, are associated with increased odds of shellfish allergy
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Elissa M. Abrams, Jackie Gruber, Cathérine Lemoine-Courcelles, Jennifer Gerdts, Jennifer L.P. Protudjer, and Elinor Simons
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Race ethnicity ,business.industry ,Shellfish allergy ,MEDLINE ,Shellfish Hypersensitivity ,medicine.disease ,Odds ,Socioeconomic Factors ,Environmental health ,Ethnicity ,Income ,medicine ,Humans ,Immunology and Allergy ,business - Published
- 2021
29. Myosin Heavy Chain: An Allergen Involved in Anaphylaxis to Shrimp Head.
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Saenz de San Pedro B, López Guerrero A, Navarrete Del Pino MA, Alcántara Villar M, Álvarez F, López-Matas MA, and Carnés J
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- Humans, Myosin Heavy Chains, Allergens, Seafood, Tropomyosin, Anaphylaxis, Food Hypersensitivity
- Published
- 2023
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30. Seafood Allergy, Toxicity, and Intolerance: A Review.
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Prester, Ljerka
- Abstract
Seafood allergies have been increasing their presence in the last 2 decades. Allergic reactions to seafood can range from mild urticarial and oral allergy syndrome to life-threatening anaphylactic reactions. Ingestion of seafood infested withAnisakislarvae can cause a disease known as anisakiasis with symptoms similar to true seafood allergy. Furthermore, some adverse reactions to seafood including histamine fish poisoning (HFP), and intolerance to histamine can trigger clinical symptoms, which, although nonallergic in origin, are similar to true immunoglobulin E (IgE)-mediated allergic reactions. Because seafood allergy usually remains a lifelong food allergy, this review focuses on the current knowledge on fish and shellfish allergens and emphasizes the importance of differentiating seafood allergy from other allergy-like reactions (anisakiasis, HFP, and intolerance to histamine). Key teaching points: • Fish and shellfish are potent allergens that can provoke serious IgE antibody-mediated adverse reactions in sensitive individuals. • Sensitization to seafood allergens can be achieved by ingestion, inhalation, or skin contact. • Shellfish major allergen, tropomyosin, shares significant homology to arthropods (dust mites and cockroaches). • Accidental exposures to seafood products cross-contaminated with fish or shellfish allergens (hidden allergens) during processing may present a health risk for sensitive individuals. • Allergens of fish parasiteA. simplexpresent common hidden allergens in seafood, particularly in raw and undercooked home-made fish dishes. • Symptoms caused by HFP, histamine intolerance, and anisakiasis are similar to true seafood allergy. [ABSTRACT FROM PUBLISHER]
- Published
- 2016
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31. An Adjuvant-Free Mouse Model of Transdermal Sensitization and Oral Elicitation of Anaphylaxis to Shellfish.
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Parvataneni, Sitaram, Gonipeta, Babu, Acharya, Harini G., and Gangur, Venu
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- *
ANAPHYLAXIS , *ALLERGIES , *EXERCISE-induced anaphylaxis , *SHELLFISH , *AQUATIC invertebrates - Abstract
Background: Shellfish (SF) allergy is a leading cause of systemic anaphylaxis in humans. An adjuvant-free mouse model to evaluate allergenicity and oral anaphylaxis to SF is currently unavailable. Here, we tested the hypothesis that transdermal exposure (TDE) to SF protein extract (SFPE) not only elicits a systemic allergic immune response but also will clinically sensitize mice for oral anaphylaxis. Methods: Adult BALB/c female mice (6-8 weeks of age) were exposed to saline or SFPE once a week for 4 weeks using a transder-mal sensitization method. Systemic SF-specific IgE, IgG1 and IgG2a and total (t)IgE responses were measured using ELISA. Systemic anaphylaxis upon oral SFPE administration was assessed according to clinical symptoms and the hypothermia shock response (HSR). Using individual mouse data, the correlation between the readouts of allergenicity was determined using Pearson's analysis. Spleen-cell IL-4 and IFN-γ responses were determined using primary cell culture and ELISA. Results:TDE to SFPE resulted in marked systemic specific (s)IgE, tIgE, IgG1 and IgG2a responses. Oral challenge with SFPE in sensitized mice (but not controls) elicited systemic anaphylactic clinical reactions and HSR. A strong correlation was observed between sIgE, tIgE and HSR. Spleen cells isolated from allergic mice (but not controls) exhibited memory IL-4 and IFN-γ cytokine responses. Conclusion: We report a novel adjuvant-free mouse model of SF allergy with robust quantifiable and correlated readouts of allergenicity that may be used in basic biomedical, preclinical and applied food/nutrition research on SF allergy. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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32. Detection and characterization of tropomyosin from Anadara Seashells using immunoproteomic aproach
- Author
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Mladenović, Maja, Romanyuk, Nataliya, Smiljanić, Katarina, Jovanović, Vesna B., Ćirković-Veličković, Tanja, Mladenović, Maja, Romanyuk, Nataliya, Smiljanić, Katarina, Jovanović, Vesna B., and Ćirković-Veličković, Tanja
- Abstract
Introduction: Shellfish allergy is one of the most common food allergies with a prevalence of 0.5%-2.5% in the general population. The most common allergen present in shellfish is tropomyosin. Detection of tropomyosin in seashells is a challenge because there are no specific antibodies for seashells’ tropomyosin. Our aim was to verify the presence of tropomyosin in Anadara seashells using an immunoproteomic approach and to investigate the level of cross-reactivity with shrimps.Methods and Results: Proteins from lyophilized seashells Tegillarca granosa (TG) and Anadara broughtonii (AB) were extracted in: RIPA buffer (1% Triton X-100, 1% sodium deoxycholate, 0,1% SDS, 150 mM NaCl, 50 mM Tris-HCl, 1mM EDTA) and Rehydration buffer (7M urea, 2M thiourea, 2% CHAPS and 10mM DTT). Protein concentration of extracts was determined by Bradford assay and SDS-PAGE. The presence of tropomyosin has been supported by commercial tropomyosin standard in 1D SDS-PAGE. With 1D immunoblot, it was possible to confirm the reactivity of seashells’ tropomyosin to rabbit anti-shrimp tropomyosin polyclonal antibodies, confirming its presence. Tropomyosin’s presence was also validated with 1D immunoblot using monoclonal antibodies. 2D electrophoresis showed that most of samples’ proteins are in acidic pI range with prevalence of spots in the range 35-50kDa, and, by comparing spots to 2D immunoblot with polyclonal antibodies, it is possible to confirm tropomyosin’s presence in Anadara seashells.Conclusions: We found that tropomyosin is present in both blood clam species. Both monoclonal and polyclonal antibodies raised against shrimp tropomyosin can detect seashells tropomyosin by immunoblot pointing to a potential antibodies cross-reactivity of allergic subjects to shrimps and seashells.
- Published
- 2021
33. Isolation and Cloning of Tropomyosin and Arginine Kinase from Tiger Prawn Penaeus monodon and Blue Swimming Crab Portunus trituberculatus
- Author
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Amelia Suhana Zamri, Zailatul Hani Mohamad Yadzir, and Brenda Leecyous
- Subjects
biology ,Shellfish allergy ,food and beverages ,Portunus trituberculatus ,Arginine kinase ,biology.organism_classification ,medicine.disease ,Tropomyosin ,Penaeus monodon ,Microbiology ,medicine ,Actin filament binding ,Prawn ,biology.protein ,Shellfish - Abstract
Shellfish is an important source of food and plays a significant role in human nutrition and health. However, shellfish allergy is a long-lasting disorder which mostly persists throughout life and is often associated with severe reactions [1]. Among the various consumed shellfish, prawns and crabs are the most widely consumed and can lead to the most severe reactions. At present, allergies to shellfish are diagnosed similarly to other food allergies. The diagnosis relies upon careful evaluation of history, the presence of appropriate clinical signs and confirmation with in vivo or in vitro tests to demonstrate the presence of allergen-specific immunoglobulin E (IgE) [2]. However, both in vivo or in vitro diagnostic approaches are mainly based on the use of crude allergen extracts. Crude allergen extracts are obtained from biological sources and consist of mixture of allergenic components with high amounts of undesirable products that can interfere with diagnosis. In many cases, only few of the several proteins found in crude allergen extracts act as the essential allergens in the majority of patients that are allergic to the substance. The most important ones are called major allergens. Problems associated with using crude allergen extracts for allergy diagnosis may be overcome with recombinant allergens. Recombinant allergens with high purity can be produced by using controlled production procedures that yield defined molecules with known molecular, immunologic and biological characteristics [1]. Tiger prawn Penaeus monodon and blue swimming crab Portunus trituberculatus, are among the widely consumed shellfish in Malaysia. Our earlier study involving 131 atopic patients in Allergy Clinic, Kuala Lumpur Hospital demonstrated that patients in Malaysia suffering from allergic responses to shellfish including tiger prawn Penaeus monodon and blue swimming crab Portunus trituberculatus. Amongst the shellfish extracts tested, prawn elicited the highest frequency of positive reactivity in 39% of the patients. Further, crab was the second most common shellfish to elicit a positive reaction in 24% of the patients [3]. Our first phase study has successfully identified tropomyosin and arginine kinase as the major allergens in both species of shellfish. However, more information about the individual allergenic species-specific components is needed. Therefore, we continued our study to isolate and clone the tropomyosin and arginine kinase from these two species of shellfish, tiger prawn Penaeus monodon and blue swimming crab Portunus trituberculatus. Tropomyosin and arginine kinase were isolated from the total RNA (Ribonucleic Acid) obtained from both prawn and crab muscles followed by RT-PCR (Reverse Transcriptase-Polymerase Chain Reaction). The RT-PCR products were then cloned into the cloning vector, pJET 1.2 and transformed into Escherichia coli host. Transformants were screened for positive clones by PCR (Polymerase Chain Reaction) colony and sequenced. The 855 bp tropomyosins have been isolated and sequenced from both prawn and crab. Arginine kinases isolated and sequenced from prawn and crab were 1071 bp and 1074 bp, respectively (Figure 1). The GenBank BLAST search for the sequences showed high homology to the targeted proteins as shown in Table 1. Tropomyosin is a 34 to 38 kDa heat-stable protein that belongs to a highly conserved family of actin filament binding proteins, which plays a functional role in contractile activities in muscle cells [4]. Arginine kinase is a 40 to 42 kDa heat-labile protein that plays an important role in regenerating adenosine triphosphate (ATP) during bursts of cellular activity [5]. Tropomyosin and arginine kinase from the prawn and crab have been isolated and the full-length sequences were obtained. Current ongoing study focuses on sub-cloning and full-length expression of tropomyosin and arginine kinase in order to produce respective recombinant proteins, and subsequently investigate their physicochemical and allergenic characteristics.
- Published
- 2020
34. Development of a sandwich ELISA and immunochromatographic strip for the detection of shrimp tropomyosin
- Author
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Luo Pengjie, Wu Xiaoling, Hua Kuang, Shanshan Song, Qiankun Zheng, and Lu Zeng
- Subjects
lcsh:Immunologic diseases. Allergy ,animal structures ,medicine.drug_class ,Immunology ,macromolecular substances ,Biology ,Monoclonal antibody ,01 natural sciences ,Microbiology ,0404 agricultural biotechnology ,medicine ,shrimp tropomyosin ,lcsh:Agriculture (General) ,medicine.diagnostic_test ,010401 analytical chemistry ,fungi ,sandwich elisa ,Shellfish allergy ,food and beverages ,04 agricultural and veterinary sciences ,medicine.disease ,040401 food science ,Tropomyosin ,lcsh:S1-972 ,0104 chemical sciences ,Shrimp ,Highly sensitive ,immunochromatographic assay strip ,monoclonal antibody ,Immunoassay ,lcsh:RC581-607 ,Agronomy and Crop Science ,Food Science - Abstract
Shrimp tropomyosin is one of the most important causes of shellfish allergy. The objective of this study was to develop a highly sensitive and specific immunoassay for the detection of shrimp tropomyosin in food. Ten monoclonal antibodies against shrimp tropomyosin were obtained by fusion and cell screening. We found two optimum monoclonal antibodies (mAbs); mAb 2 was used as the capture antibody, and HRP-labelled mAb 3 was used as the detection antibody. Using this pair of mAbs, a sandwich enzyme-linked immunosorbent assay (ELISA) was developed with the limit of detection (LOD) of 0.45 ng/mL. Cross-reactivity with other food allergens using this method was found to be negligible. An immunochromatographic assay strip was also developed for the rapid detection of shrimp tropomyosin with the LOD of 0.5 ng/mL. The results demonstrated that our developmental methods represent a useful tool for the detection of shrimp tropomyosin in food.
- Published
- 2019
35. Structural and allergenic properties of the fatty acid binding protein from shrimp Litopenaeus vannamei
- Author
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Leonardo Puerta, Marlon Munera, Luis Caraballo, Dalgys Martínez, Josefina Zakzuk, Judith Wortmann, and Walter Keller
- Subjects
Immunology ,Cross Reactions ,Immunoglobulin E ,medicine.disease_cause ,Fatty Acid-Binding Proteins ,law.invention ,Allergen ,Penaeidae ,law ,Food allergy ,Tandem Mass Spectrometry ,medicine ,Immunology and Allergy ,Animals ,Humans ,biology ,Chemistry ,Shellfish allergy ,Allergens ,medicine.disease ,respiratory tract diseases ,Shrimp ,Basophil activation ,Epitope mapping ,Biochemistry ,biology.protein ,Recombinant DNA ,Food Hypersensitivity ,Chromatography, Liquid - Abstract
Background The shrimp Litopenaeus vannamei is an important source of food allergens but its allergenic repertoire is poorly characterized. Cross-reactivity between crustacean and mites has been reported, with tropomyosin, the most relevant allergen involved. The aim of this study was to investigate the structural and immunological properties of a recombinant Fatty Acid Binding Protein (FABP) family from L. vannamei (LvFABP). Methods ELISA, skin prick test (SPT) and basophil activation assays were performed to determine IgE reactivity and allergenic activity of LvFABP. LC-MS/MS and Circular Dichroism experiments were done for structural analysis. B-cell epitope mapping with overlapping peptides, and cross-inhibition studies using human sera were done to identify antigenic regions and cross-reactivity. Results The recombinant LvFABP bound serum IgE from 27% of 36 shrimp allergic patients and showed allergenic activity when tested for basophil activation and SPT in a selected number of them. CD-spectroscopy of LvFABP revealed that the protein is folded with a secondary structure composed of mainly β-strands and a smaller fraction of α helices. This is consistent with molecular modelling results, which exhibit a typical β barrel fold with two α-helices and ten β-strands. Epitope mapping identified two IgE-binding antigenic regions and inhibition assays found high cross-reactivity between LvFABP and Blo t 13, mediated by the antigenic region involving amino acids 54 to 72. Conclusions Our results show that LvFABP is a shrimp allergen that cross reacts with the house dust mite allergen Blo t 13 and has allergenic activity, which suggest that it could be clinically relevant in case of shellfish allergy. This new allergen, named Lit v 13, will also help to understand basic mechanisms of sensitization to shrimp.
- Published
- 2021
36. Shellfish Allergy: a Comprehensive Review.
- Author
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Pedrosa, María, Boyano-Martínez, Teresa, García-Ara, Carmen, and Quirce, Santiago
- Abstract
Shellfish allergy is of increasing concern, as its prevalence has risen in recent years. Many advances have been made in allergen characterization. B cell epitopes in the major allergen tropomyosin have been characterized. In addition to tropomyosin, arginine kinase, sarcoplasmic calcium-binding protein, and myosin light chain have recently been reported in shellfish. All are proteins that play a role in muscular contraction. Additional allergens such as hemocyanin have also been described. The effect of processing methods on these allergens has been studied, revealing thermal stability and resistance to peptic digestion in some cases. Modifications after Maillard reactions have also been addressed, although in some cases with conflicting results. In recent years, new hypoallergenic molecules have been developed, which constitute a new therapeutic approach to allergic disorders. A recombinant hypoallergenic tropomyosin has been developed, which opens a new avenue in the treatment of shellfish allergy. Cross-reactivity with species that are not closely related is common in shellfish-allergic patients, as many of shellfish allergens are widely distributed panallergens in invertebrates. Cross-reactivity with house dust mites is well known, but other species can also be involved in this phenomenon. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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37. Risk factors in pediatric shrimp allergy.
- Author
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Chokshi, Niti Y., Maskatia, Zahida, Miller, Sarah, Guffey, Danielle, Minard, Charles G., and Davis, Carla M.
- Subjects
SHRIMPS ,IMMUNOLOGIC diseases ,FOOD allergy ,PEDIATRICS - Abstract
Background: The prevalence of shellfish allergy is ∼1.3% in the United States, with shrimp most commonly reported. Shellfish is one of the top causes of food-induced anaphylactic reactions, yet there are no reported rates of pediatric shrimp anaphylaxis in the literature. In previously reported adults with shrimp allergy, the rate of anaphylaxis to shrimp was 42%. Objective: To describe the rate of anaphylaxis among children with shrimp allergy, demographics, clinical presentation, and cross-reactive sensitization. Methods: Retrospective chart review of children ≤18 years old who presented with shrimp allergy to Texas Children's Hospital Allergy and Immunology Clinic over 11 years. Results: Sixty-eight patients were identified with shrimp allergy (61% male, 39% female), with a median age of diagnosis at 5.6 years (range, 0.96-16.6 years). The rate of anaphylaxis was 12%, and mucocutaneous symptoms were most common (skin symptoms, 70%; urticaria, 58%; and angioedema, 58%). No factors were positively associated with anaphylaxis, whereas patients without anaphylaxis had a significantly higher rate of eczema compared with those with anaphylaxis (p = 0.02). African Americans and Asian Americans were disproportionately affected (p < 0.001). There were low rates of cross-sensitization for other crustaceans and for mollusks (57% and 26%, respectively). Conclusion: The rate of anaphylaxis to shrimp was significantly lower in children with shrimp allergy than in adults, and anaphylactic reactions were negatively associated with eczema. Cross-reactivity to other crustaceans and mollusks does not uniformly occur. Prospective studies with double blinded placebo-controlled food challenges are needed to further characterize patients with shrimp allergy. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
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38. The gut microbiota, environmental factors, and links to the development of food allergy
- Author
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Lee, Khui Hung, Song, Yong, Wu, Weidong, Yu, Kan, Zhang, Guicheng, Lee, Khui Hung, Song, Yong, Wu, Weidong, Yu, Kan, and Zhang, Guicheng
- Abstract
Food allergy appears to have its roots in an insufficient exposure to a diverse range of environmental microbiota during early life. Microbial exposure ensures the colonization of the gastrointestinal tract with commensal microbes, which is necessary for the induction of a balanced and tolerogenic immune function. High-throughput sequencing technology has facilitated in-depth studies of the gut microbiota as well as bacterial-derived metabolites. Although the role of the microbiota in allergies is now widely studied, its importance for food allergy was only recently noted. Studies in human cohorts have shown that there is an association of dysbiosis and pathogenesis of food allergy, while studies from animal models have demonstrated the capacity of specific species in the gut microbiota to alter immune response, which may lead to the desensitization of food allergy. This article reviews the role of the gut microbiota in food allergy, and discusses the influence of environmental factors as well as prevention and management strategies relating to such regulatory mechanism. © 2020 The Author(s).
- Published
- 2020
39. Usefulness of the Nasal Allergen Provocation Test in the Diagnosis of Shellfish Allergy.
- Author
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Gelis S, Rueda M, Pascal M, Fernández-Caldas E, Fernández EA, Araujo-Sánchez G, Bartra J, and Valero A
- Subjects
- Humans, Allergens, Immunoglobulin E, Nasal Provocation Tests, Skin Tests, Plant Extracts, Shellfish Hypersensitivity diagnosis, Anaphylaxis
- Abstract
Background and Objectives: Shellfish allergy is a major cause of food allergy and anaphylaxis worldwide. Several allergenic proteins have been described in the last few years, but the only diagnostic tool that still enables discrimination between allergic and nonallergic sensitized persons is the oral food challenge (OFC). The aim of this study was to evaluate the usefulness of the nasal allergen provocation test (NAPT) as a diagnostic tool in shellfish allergy., Methods: Forty-five patients with confirmed sensitization to shrimp by a positive skin prick test (SPT) result with a commercial shrimp extract were recruited and classified as sensitized-allergic or sensitized-nonallergic based on current tolerance to shrimp intake, the result of an OFC with a freeze-dried cooked shrimp mixture extract, or a recent history of anaphylaxis induced by shrimp ingestion. These patients and 10 controls not sensitized to shrimp underwent NAPT with a freeze-dried cooked shrimp mixture extract. The response was evaluated using acoustic rhinometry and a visual analog scale., Results: Significant differences (P=.001) were found between the sensitized-allergic group (18/20 positive NAPT, 90%) and both the sensitized-nonallergic group (2/18 positive NAPT, 11.1%) and controls (0/10 positive NAPT). NAPT enables differentiation between allergic and nonallergic persons with a sensitivity of 90%, specificity of 89%, positive predictive value of 90%, and negative predictive value of 89%., Conclusions: Our results indicate that NAPT makes it possible to differentiate between sensitized symptomatic patients and sensitized tolerant patients and could be a valuable diagnostic tool when assessing shrimp allergy.
- Published
- 2022
- Full Text
- View/download PDF
40. House dust mite sensitization, eczema, and wheeze increase risk of shellfish sensitization
- Author
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Oon Hoe Teoh, Keith M. Godfrey, Hugo Van Bever, Lynette Pei-Chi Shek, Kok Hian Tan, Bee Wah Lee, Noor H. A. Suaini, Evelyn Xiu Ling Loo, Elizabeth Huiwen Tham, Hui Xing Lau, Yap Seng Chong, Johan G. Eriksson, Lydia Su Yin Wong, and Anne Eng Neo Goh
- Subjects
Dermatophagoides pteronyssinus ,Immunology ,Eczema ,medicine.disease_cause ,Article ,03 medical and health sciences ,0302 clinical medicine ,Allergen ,Wheeze ,medicine ,Mite ,Immunology and Allergy ,Animals ,Humans ,030212 general & internal medicine ,Sensitization ,Shellfish ,Respiratory Sounds ,House dust mite ,Mites ,biology ,business.industry ,Pyroglyphidae ,technology, industry, and agriculture ,Shellfish allergy ,food and beverages ,Dust ,Allergens ,medicine.disease ,biology.organism_classification ,respiratory tract diseases ,medicine.anatomical_structure ,030228 respiratory system ,Pediatrics, Perinatology and Child Health ,medicine.symptom ,business ,Anaphylaxis - Abstract
Shellfish allergy is more prevalent in South-East Asia (~5%1 ) than in Western populations (e.g. United States ~2-3%.2 ). Typically commencing in late childhood, it is the leading cause of anaphylaxis in adolescents and adults in Asia. Cross-sectional studies have demonstrated a high correlation between shellfish and house dust mite (HDM) sensitization in warm, humid climates and urban environments where HDM are ubiquitous. The major shellfish allergen, tropomyosin, shares ~80% structural homology with HDM tropomyosin, and primary sensitization to dust mite tropomyosin has been hypothesized to induce cross-sensitization to shellfish.
- Published
- 2021
41. Novel Allergen Discovery through Comprehensive De Novo Transcriptomic Analyses of Five Shrimp Species
- Author
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Shaymaviswanathan Karnaneedi, Sandip D. Kamath, Aya C Taki, Dean R. Jerry, Roni Nugraha, Elecia B. Johnston, Roger Huerlimann, Andreas L. Lopata, Thimo Ruethers, and Nicholas M. Wade
- Subjects
0301 basic medicine ,Tropomyosin ,Penaeus monodon ,lcsh:Chemistry ,0302 clinical medicine ,immune system diseases ,RNA-Seq ,lcsh:QH301-705.5 ,Spectroscopy ,Phylogeny ,education.field_of_study ,biology ,Shellfish allergy ,General Medicine ,respiratory system ,Computer Science Applications ,Shrimp ,Prawn ,Food Hypersensitivity ,allergen ,prawn ,Penaeidae ,animal structures ,Population ,Zoology ,Cross Reactions ,Catalysis ,Article ,Arthropod Proteins ,Inorganic Chemistry ,Evolution, Molecular ,03 medical and health sciences ,Species Specificity ,Whiteleg shrimp ,medicine ,Animals ,Humans ,14. Life underwater ,Physical and Theoretical Chemistry ,education ,Molecular Biology ,Shellfish ,Gene Expression Profiling ,Organic Chemistry ,fungi ,Allergens ,medicine.disease ,biology.organism_classification ,allergy ,respiratory tract diseases ,030104 developmental biology ,030228 respiratory system ,lcsh:Biology (General) ,lcsh:QD1-999 ,Seafood ,Transcriptome - Abstract
Shellfish allergy affects 2% of the world&rsquo, s population and persists for life in most patients. The diagnosis of shellfish allergy, in particular shrimp, is challenging due to the similarity of allergenic proteins from other invertebrates. Despite the clinical importance of immunological cross-reactivity among shellfish species and between allergenic invertebrates such as dust mites, the underlying molecular basis is not well understood. Here we mine the complete transcriptome of five frequently consumed shrimp species to identify and compare allergens with all known allergen sources. The transcriptomes were assembled de novo, using Trinity, from raw RNA-Seq data of the whiteleg shrimp (Litopenaeus vannamei), black tiger shrimp (Penaeus monodon), banana shrimp (Fenneropenaeus merguiensis), king shrimp (Melicertus latisulcatus), and endeavour shrimp (Metapenaeus endeavouri). BLAST searching using the two major allergen databases, WHO/IUIS Allergen Nomenclature and AllergenOnline, successfully identified all seven known crustacean allergens. The analyses revealed up to 39 unreported allergens in the different shrimp species, including heat shock protein (HSP), alpha-tubulin, chymotrypsin, cyclophilin, beta-enolase, aldolase A, and glyceraldehyde-3-phosphate dehydrogenase (G3PD). Multiple sequence alignment (Clustal Omega) demonstrated high homology with allergens from other invertebrates including mites and cockroaches. This first transcriptomic analyses of allergens in a major food source provides a valuable resource for investigating shellfish allergens, comparing invertebrate allergens and future development of improved diagnostics for food allergy.
- Published
- 2020
42. T-Cell Epitope Immunotherapy in Mouse Models of Food Allergy
- Author
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Ka Hou Chu, Christine Y.Y. Wai, Nicki Y.H. Leung, and Patrick S.C. Leung
- Subjects
biology ,business.industry ,medicine.medical_treatment ,T cell ,Shellfish allergy ,FOXP3 ,Immunotherapy ,Immunoglobulin E ,medicine.disease ,Epitope ,medicine.anatomical_structure ,Food allergy ,Egg allergy ,Immunology ,medicine ,biology.protein ,business - Abstract
Food allergy has been rising in prevalence over the last two decades, affecting more than 10% of the world population. Current management of IgE-mediated food allergy relies on avoidance and rescue medications; research into treatments that are safer and providing guaranteed and durable curative effects is, therefore, essential. T-cell epitope-based immunotherapy holds the potential for modulating food allergic responses without IgE cross-linking. In this chapter, we describe the methods in evaluating the therapeutic capacities of immunodominant T-cell epitopes in animal models of food allergy. Moreover, we explain in detail the methods to measure the allergen-specific antibody levels, prepare single-cell suspension from spleen, and prepare small intestine for immunohistochemical analysis of eosinophils and Foxp3+ cells.
- Published
- 2020
43. SWATH-MS-based proteomics reveals functional biomarkers of Th1/Th2 responses of tropomyosin allergy in mouse models.
- Author
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Li Xu, Li, Wei Zhang, Hong, Lin, Hong, Mei Zhang, Xiao, Qi Wen, Yun, Long Zhao, Jin, Xing Li, Zhen, and Gasset, María
- Subjects
- *
TROPOMYOSINS , *PROTEOMICS , *LABORATORY mice , *FOOD allergy , *ALLERGIES - Abstract
• SWATH-MS-based proteomics described the splenic and intestinal traits of tropomyosin allergy. • In the spleen, allergy is characterized by the upregulation of the Ig κ chain V-III region PC 7175. • In the intestine, allergy concurred with the downregulated expression of 35 proteins. • The downregulated proteins are involved in metabolic, absorption and wound-healing processes. Type-I food allergies are hypersensitive reactions compromising the immune organs and epithelial barriers. To investigate the organ-specific proteomic alterations of the allergy responses, the spleen and intestine of mice sensitized with high (shrimp and clam) and weak (fish) allergenic tropomyosins were analyzed using sequential windowed acquisition of all theoretical fragment ion mass spectra (SWATH-MS)-based proteomics. The results showed that Th1 and Th2 tropomyosin-induced responses in the spleen are characterized by the unique upregulation of innate (cochlin) and adaptive (Ig κ chain V-III region PC 7175) immune regulators, respectively. In the intestine, tropomyosin allergy concurred with the downregulation of 35 differentiating proteins featuring the overall impairment of metabolic pathways, absorption processes and ammonium ion responses. These data provide new functional biomarkers of tropomyosin-induced immune responses as well as candidate targets for intervention. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
44. Validation and Reliability of the Thai version of the Food Allergy Quality of Life Questionnaire-Parent Form (FAQLQ-PF)
- Author
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Punchama Pacharn, Wannaporn Limpitikul, Orathai Jirapongsananuruk, Witchaya Srisuwatchari, and Nualanong Visitsunthorn
- Subjects
medicine.medical_specialty ,business.industry ,Intraclass correlation ,Immunology ,Shellfish allergy ,Discriminant validity ,General Medicine ,Guideline ,medicine.disease ,Quality of life ,Cronbach's alpha ,Food allergy ,Family medicine ,parasitic diseases ,medicine ,Immunology and Allergy ,business ,Wheat allergy - Abstract
Background Food allergy has an impact on the quality of life of both patients and caregivers. It is, therefore, important to have a native language survey to evaluate health-related quality of life (HRQL) among food allergic children. Objective To translate the Food Allergy Quality of Life Questionnaire-Parent Form (FAQLQ-PF) to Thai language, and to validate this tool in Thai parents with food allergic children. Methods The FAQLQ-PF was translated into Thai language according to WHO guideline. The FAQLQ-PF Thai version was then administered to the parents of food allergic Thai children aged 0-12 years. The FAQLQ-PF Thai version was then readministered to those same parents 10-14 days after they first completed this assessment tool. Internal consistency by Cronbach's α and test-retest reliability by intraclass correlation coefficient (ICC) were assessed. The discriminant validity of the questionnaire was also evaluated. Results Ninety parents of participants answered the FAQLQ-PF Thai version. Of those, 9 parents (10%) incompletely answered the first questionnaire. The FAQLQ-PF Thai version showed good internal consistency (Cronbach's α ≥ 0.799), but the test-retest reliability was only fair (ICC > 0.6). Factors that adversely affected the quality of life of Thai children with food allergy included age, presence of anaphylaxis, frequency of reactions, and the number of implicated foods. Patients with wheat allergy were negatively impacted in all domains of quality of life, whereas those with shellfish allergy had only emotional impact. Conclusions The FAQLQ-PF Thai version is a reliable and valid tool for assessing HRQL in Thai children with food allergy.
- Published
- 2020
45. Shellfish Allergy: Unmet Needs in Diagnosis and Treatment
- Author
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A Valero, S Gelis, Enrique Fernández-Caldas, M Rueda, E A Fernández, and M Moran
- Subjects
0301 basic medicine ,Allergy ,Immunology ,Population ,Clinical Decision-Making ,Shellfish Hypersensitivity ,Cross Reactions ,Food group ,03 medical and health sciences ,Epitopes ,0302 clinical medicine ,Food allergy ,Risk Factors ,Environmental health ,medicine ,Immunology and Allergy ,Animals ,Humans ,Adverse effect ,education ,Shellfish ,education.field_of_study ,business.industry ,Shellfish allergy ,food and beverages ,Disease Management ,Allergens ,Immunoglobulin E ,medicine.disease ,030104 developmental biology ,030220 oncology & carcinogenesis ,Disease Susceptibility ,Symptom Assessment ,business ,Anaphylaxis ,Algorithms - Abstract
Seafood is a major cause of food allergy and anaphylaxis worldwide. Shellfish is included among the “big eight” food groups, which are responsible for more than 90% of all cases of food allergy. Approximately 2.5% of the world’s population has experienced an adverse reaction to seafood. Seafood allergy is one of the most frequent and lethal allergies that exist. The several allergenic proteins involved in allergic reactions that have been described in recent years include tropomyosin, arginine kinase, myosin light chain, and sarcoplasmic calcium-binding protein. Despite all the data reported in the last few years, shellfish allergy is still diagnosed and treated as it was 50 years ago. The only effective treatment to prevent allergic reactions to shellfish is avoidance. This review aims to update recently published data on shellfish allergy and to highlight those areas that have yet to be resolved.
- Published
- 2020
46. Novel allergen discovery through comprehensivede novotranscriptomic analyses of 5 shrimp species
- Author
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Shaymaviswanathan Karnaneedi, Nicholas M. Wade, Elecia B. Johnston, Roger Huerlimann, Andreas L. Lopata, Roni Nugraha, Thimo Ruethers, Sandip D. Kamath, Dean R. Jerry, and Aya C Taki
- Subjects
animal structures ,biology ,fungi ,Shellfish allergy ,Litopenaeus ,Zoology ,biology.organism_classification ,medicine.disease ,medicine.disease_cause ,respiratory tract diseases ,Penaeus monodon ,Shrimp ,Allergen ,Food allergy ,Whiteleg shrimp ,medicine ,Shellfish - Abstract
Shellfish allergy affects up to 2% of the world’s population and persists for life in most patients. The diagnosis of a shellfish allergy, in particular shrimp, is however often challenging due to the similarity of allergenic proteins in other invertebrates. Despite the clinical importance, the complete allergen repertoire of allergy-causing shrimps remains unclear. Here we mine the complete transcriptome of five frequently consumed shrimp species to identify and compare allergens with all known allergen sources. The transcriptomes were assembledde novofrom raw RNA-Seq data of the whiteleg shrimp (Litopenaeus vannamei), black tiger shrimp (Penaeus monodon), banana shrimp (Fenneropenaeus merguiensis), king shrimp (Melicertus latisulcatus), and endeavour shrimp (Metapenaeus endeavouri). Trinity was used to assemble the transcriptome, and Transrate and BUSCO applied to verify the assembly. Blast search with the two major allergen databases, WHO/IUIS Allergen Nomenclature and AllergenOnline, successfully identified all seven known crustacean allergens. Salmon was utilised to measure their relative abundance, demonstrating sarcoplasmic calcium-binding protein, arginine kinase and myosin light chain as highly abundant allergens. In addition, the analyses revealed up to 40 unreported allergens in different shrimp species, including heat shock protein (HSP), alpha-tubulin, chymotrypsin, cyclophilin, beta-enolase, aldolase A, and glyceraldehyde-3-phosphate dehydrogenase (G3PD). Multiple sequence alignment, conducted in Jalview 2.1 with Clustal Omega, demonstrated high homology with allergens from other invertebrates including mites and cockroaches. This first transcriptomic analyses of allergens in a major food source provides a valuable genomic resource for investigating shellfish allergens, comparing invertebrate allergens and developing improved diagnostics and novel immunotherapeutics for food allergy.
- Published
- 2020
47. Cell-Based Functional IgE Assays Are Superior to Conventional Allergy Tests for Shrimp Allergy Diagnosis
- Author
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Jinlyu Sun, Kary J.Y. Xu, Patrick S.C. Leung, Nicki Y.H. Leung, Joshua S.C. Wong, Yuki Shum, Ka Hou Chu, Christine Y.Y. Wai, Pui Fung Li, Agnes Sze Yin Leung, Cheuk Yin Lam, Ting Fan Leung, Gary W.K. Wong, Yat Wah Kwan, Ivan C.S. Lam, and Qun Ui Lee
- Subjects
Allergy ,animal structures ,Tropomyosin ,Immunoglobulin E ,medicine.disease_cause ,03 medical and health sciences ,0302 clinical medicine ,Allergen ,medicine ,Immunology and Allergy ,Animals ,Humans ,030212 general & internal medicine ,SCORAD ,Skin Tests ,biology ,medicine.diagnostic_test ,business.industry ,fungi ,Shellfish allergy ,Gold standard (test) ,Allergens ,medicine.disease ,Shrimp ,Basophil activation ,030228 respiratory system ,Immunology ,biology.protein ,business ,Food Hypersensitivity - Abstract
Background The diagnosis of shellfish allergy currently relies on patient history, skin prick test (SPT), and serum specific IgE (sIgE) quantification. These methods lack sufficient diagnostic accuracy, whereas the gold standard of oral food challenges is risky and burdensome. Markers of reactivity and severity of allergic reactions to shellfish will improve clinical care of these patients. Objectives This study compared the diagnostic performance of SPT, sIgE, basophil activation test (BAT), and IgE crosslinking–induced luciferase expression (EXiLE) test for shrimp allergy. Methods Thirty-five subjects with documented history of shrimp allergic reactions were recruited and grouped according to results of double-blind, placebo-controlled food challenge (DBPCFC). In addition to routine diagnostics, BAT (Flow CAST) and EXiLE test with shrimp extract and tropomyosin were performed. Results Of 35 subjects, 15 were shrimp allergic with pruritus, urticaria, and itchy mouth on DBPCFC, whereas 20 were tolerant to shrimp. Tropomyosin only accounted for 53.3% of sensitization among subjects with challenge-proven shrimp allergy. BAT using shrimp extract as stimulant showed the highest area under curve value (0.88), Youden Index (0.81), likelihood ratio (14.73), odds ratio (104), and variable importance (4.27) when compared with other assays and tropomyosin diagnosis. Results of BAT significantly correlated with those of EXiLE (r = 0.664, P Conclusions BAT is a more accurate diagnostic marker for shrimp allergy than SPT and shrimp sIgE, whereas the EXiLE test based on an IgE crosslinking assay is a good alternative to BAT. Tropomyosin may not be the most important shrimp allergen in Chinese, which warrants further investigation to search for other major allergens and diagnostic markers.
- Published
- 2020
48. Overcoming Shellfish Allergy: How Far Have We Come?
- Author
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Patrick S.C. Leung, Christine Y.Y. Wai, Nicki Y.H. Leung, Ka Hou Chu, Ting Fan Leung, Gary W.K. Wong, and Agnes Sze Yin Leung
- Subjects
0301 basic medicine ,component-resolved diagnosis ,diagnosis ,Review ,Desensitization ,lcsh:Chemistry ,tropomyosin ,0302 clinical medicine ,Autoinjector ,Immunologic ,Prevalence ,Medicine ,lcsh:QH301-705.5 ,Spectroscopy ,Vaccines ,Shellfish allergy ,Treatment options ,food and beverages ,Disease Management ,allergen-specific immunotherapy ,General Medicine ,Foodborne Illness ,Combined Modality Therapy ,Computer Science Applications ,Disease Susceptibility ,DNA vaccine ,medicine.medical_specialty ,Standard of care ,Diagnostic methods ,animal structures ,Food Allergies ,Shellfish Hypersensitivity ,Cross Reactions ,Catalysis ,Inorganic Chemistry ,03 medical and health sciences ,Clinical Research ,Food allergy ,Genetics ,Animals ,Humans ,Physical and Theoretical Chemistry ,Intensive care medicine ,Molecular Biology ,Shellfish ,Nutrition ,Molecular identification ,Chemical Physics ,business.industry ,Prevention ,Inflammatory and immune system ,Organic Chemistry ,technology, industry, and agriculture ,Immunoglobulin E ,Allergens ,medicine.disease ,030104 developmental biology ,lcsh:Biology (General) ,lcsh:QD1-999 ,030228 respiratory system ,Seafood ,Desensitization, Immunologic ,Immunization ,Other Biological Sciences ,Digestive Diseases ,business ,Other Chemical Sciences - Abstract
Shellfish allergy caused by undesirable immunological responses upon ingestion of crustaceans and mollusks is a common cause of food allergy, especially in the Asia-Pacific region. While the prevalence of shellfish allergy is increasing, the mainstay of clinical diagnosis for these patients includes extract-based skin prick test and specific IgE measurement while clinical management consists of food avoidance and as-needed use of adrenaline autoinjector should they develop severe allergic reactions. Such a standard of care is unsatisfactory to both patients and healthcare practitioners. There is a pressing need to introduce more specific diagnostic methods, as well as effective and safe therapies for patients with shellfish allergy. Knowledge gained on the identifications and defining the immuno-molecular features of different shellfish allergens over the past two decades have gradually translated into the design of new diagnostic and treatment options for shellfish allergy. In this review, we will discuss the epidemiology, the molecular identification of shellfish allergens, recent progress in various diagnostic methods, as well as current development in immunotherapeutic approaches including the use of unmodified allergens, hypoallergens, immunoregulatory peptides and DNA vaccines for the prevention and treatment of shellfish allergy. The prospect of a “cure “for shellfish allergy is within reach.
- Published
- 2020
49. The predominance of seafood allergy in Vietnamese adults: Results from the first population-based questionnaire survey
- Author
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An T.L. Vu, Huong T.M. Ho, Thuy T.B. Tran, Thu T.K. Le, Andreas L. Lopata, and Emma S. McBryde
- Subjects
Pulmonary and Respiratory Medicine ,lcsh:Immunologic diseases. Allergy ,Immunology ,Population ,HREC, Human Research Ethics Committee ,Seafood allergy ,Article ,Food group ,03 medical and health sciences ,EAACI, European Academy of Allergy and Clinical Immunology ,0302 clinical medicine ,Food allergy ,medicine ,Prevalence ,Immunology and Allergy ,Adults ,Family history ,FA, Food allergy ,030223 otorhinolaryngology ,education ,2. Zero hunger ,education.field_of_study ,business.industry ,Incidence (epidemiology) ,Shellfish allergy ,Odds ratio ,IQR, Interquartile range ,medicine.disease ,3. Good health ,030228 respiratory system ,Vietnam ,Tree nut allergy ,business ,lcsh:RC581-607 ,WAO, World Allergy Organization ,Demography ,OR, Odds ratio - Abstract
Background: Food allergy (FA) is a serious, costly and growing health problem worldwide. FA occurs in both children and adults; however, there is a paucity of information on FA prevalence and its clinical features in the adult population, especially in Asia. We sought to assess the prevalence of FAs in Vietnamese adults and the distribution of offending food items among different regions throughout Vietnam. Methods: A nationwide, cross-sectional, population-based survey was conducted among University students aged 16–50 years. We used a structured, anonymous questionnaire, which was modified from recently published FA epidemiologic studies and based on European Academy of Allergy and Clinical Immunology (EAACI) guidelines, to collect data on FA prevalence, clinical presentations, and implicated food groups. Statistical analysis was performed to generate the prevalence of self-reported and doctor-diagnosed FA and to examine the association of key environmental factors and FA incidence in this population. Results: Of the 14,500 surveys distributed, a total of 9,039 responses were returned, resulting in a response rate of 62.4%. Among participants who reported food-induced adverse reactions, 48.0% have repeated reactions. 18.0% of the participants perceived FA symptoms, but less than half of them sought medical services for confirmation (37.9%). Stratifying for true FA symptoms, the prevalence of self-reported FA was 11.8% and of doctor-diagnosed FA, 4.6%. The most common doctor-diagnosed FA was to crustacean (3.0%; 95% CI, 2.6–3.3), followed by fish (1.6%; 95% CI, 1.3–1.8), mollusk (1.3%; 95% CI, 1.0–1.5) and beef (1.0%; 95% CI, 0.8–1.2). The prevalence of doctor-diagnosed FA differed among participants living in urban (6.5%) and rural regions (4.9%) (P
- Published
- 2020
50. Structural characterization and IgE epitope analysis of arginine kinase from Scylla paramamosain.
- Author
-
Mao, Hai-Yan, Cao, Min-Jie, Maleki, Soheila J., Cai, Qiu-Feng, Su, Wen-Jin, Yang, Yang, and Liu, Guang-Ming
- Subjects
- *
IMMUNOGLOBULIN E , *EPITOPES , *ARGININE kinase , *SCYLLA (Crustacea) , *AMINO acids , *MOLECULAR weights , *ISOELECTRIC point , *ENZYME-linked immunosorbent assay - Abstract
Highlights: [•] Arginine kinase (AK) from Scylla paramamosain was purified, cloned, and expressed. [•] The IgE epitopes of S. paramamosain AK were elucidated and identified. [•] The conformational IgE epitopes of S. paramamosain AK were the predominant epitopes. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
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