Search

Your search keyword '"Shebl, Nashwa"' showing total 16 results
Start Over Author "Shebl, Nashwa"
16 results on '"Shebl, Nashwa"'

6. HBVsvp-Pulsed Dendritic Cell Immunotherapy Induces Th1 Polarization and Hepatitis B Virus-Specific Cytotoxic T Lymphocytes Production

Author

Farag, Mohamed M S, Suef, Reda A, Al-Toukhy, Ghada M, Selim, Mohamed A, Elbahnasawy, Mostafa A, El Sharkawy, Nahla, Ezzat, Sameera, Shebl, Nashwa, and Mansour, Mohamed T M

Subjects
CHB, MoDCs-pulsed-HBVsvp, Infection and Drug Resistance, immunotherapy, HBV-specific CTL, Original Research
Abstract

Mohamed M S Farag,1 Reda A Suef,1 Ghada M Al-Toukhy,2 Mohamed A Selim,1 Mostafa A Elbahnasawy,1 Nahla El Sharkawy,3 Sameera Ezzat,4 Nashwa Shebl,5 Mohamed T M Mansour6 1Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt; 2Virology & Immunology Department, Children’s Cancer Hospital, Cairo 57357, Egypt; 3Clinical Pathology Department, National Cancer Institute, Cairo University and Children Cancer Hospital, Cairo 57357, Egypt; 4Epidemiology & Preventive Medicine Department, National Liver Institute, Menoufia University, Al Minufya, Egypt; 5Hepatology Department, National Liver Institute, Menoufia University, Al Minufya, Egypt; 6Virology & Immunology Department, National Cancer Institute, Cairo University and Children Cancer Hospital, Cairo 57357, EgyptCorrespondence: Mohamed M S FaragBotany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptTel +20 2 100 670 4553Fax + 20 2 22629358Email mohamed.farag@azhar.edu.egBackground: In chronic hepatitis B virus (CHB) patients, both dendritic cells (DCs) and T cells are functionally impaired and consequently the HBV-specific cellular immune responses are downregulated. The present study aims to investigate whether monocyte-derived DC (MoDCs)-pulsed-HBV subviral particles (HBVsvp) can polarize Th1 cells to induce HBV-specific cytotoxic T-lymphocytes (CTL) responses in CHB patients.Methods and Materials: To this end, the human hepatoma HepG2.2.15 cell line was used to produce HBVsvp as a culturing system, and HBVsvp were concentrated for highly virus titer using the polyethylene glycol protocol. Peripheral blood mononuclear cells (PBMCs), collected from CHB patients and healthy donors, were differentiated into MoDCs and T cells. PBMCs-derived MoDCs were first pulsed with HBVsvp and then cultured with PBMCs-derived T cells. MoDCs and/or T subsets cells were identified for phenotypic activation by FACS analysis. The cytokine secretion of IL-4, IL-12, and IFN-γ in the culture supernatants was detected.Results: The MoDCs were restored for their activation upon pulsing with HBVsvp in vitro, as identified by significantly overexpression of both CD86 and HLA-DR, and overproduction of IL-4 and IL-12. Furthermore, MoDCs-pulsed-HBVsvp induced Th1 frequencies and activated HBV-specific CTL to produce significantly highest amount of IFN-γ. Enhanced HBV-specific CTL led to strong cytolytic capacity against HepG2.2.15.Conclusion: Overall, our data suggest that in vitro activation of MoDCs with HBVsvp overcomes the functionally impaired DCs and T cells in CHB patients offering a promising tool for therapeutic or vaccine-based approaches against HBV.Keywords: CHB, MoDCs-pulsed-HBVsvp, HBV-specific CTL, immunotherapy

Published
2020

12. HBVsvp-Pulsed Dendritic Cell Immunotherapy Induces Th1 Polarization and Hepatitis B Virus-Specific Cytotoxic T Lymphocytes Production

Author

Farag,Mohamed M S, Suef,Reda A, Al-Toukhy,Ghada M, Selim,Mohamed A, Elbahnasawy,Mostafa A, El Sharkawy,Nahla, Ezzat,Sameera, Shebl,Nashwa, Mansour,Mohamed T M, Farag,Mohamed M S, Suef,Reda A, Al-Toukhy,Ghada M, Selim,Mohamed A, Elbahnasawy,Mostafa A, El Sharkawy,Nahla, Ezzat,Sameera, Shebl,Nashwa, and Mansour,Mohamed T M

Abstract

Mohamed M S Farag,1 Reda A Suef,1 Ghada M Al-Toukhy,2 Mohamed A Selim,1 Mostafa A Elbahnasawy,1 Nahla El Sharkawy,3 Sameera Ezzat,4 Nashwa Shebl,5 Mohamed T M Mansour6 1Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt; 2Virology & Immunology Department, Children’s Cancer Hospital, Cairo 57357, Egypt; 3Clinical Pathology Department, National Cancer Institute, Cairo University and Children Cancer Hospital, Cairo 57357, Egypt; 4Epidemiology & Preventive Medicine Department, National Liver Institute, Menoufia University, Al Minufya, Egypt; 5Hepatology Department, National Liver Institute, Menoufia University, Al Minufya, Egypt; 6Virology & Immunology Department, National Cancer Institute, Cairo University and Children Cancer Hospital, Cairo 57357, EgyptCorrespondence: Mohamed M S FaragBotany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptTel +20 2 100 670 4553Fax + 20 2 22629358Email mohamed.farag@azhar.edu.egBackground: In chronic hepatitis B virus (CHB) patients, both dendritic cells (DCs) and T cells are functionally impaired and consequently the HBV-specific cellular immune responses are downregulated. The present study aims to investigate whether monocyte-derived DC (MoDCs)-pulsed-HBV subviral particles (HBVsvp) can polarize Th1 cells to induce HBV-specific cytotoxic T-lymphocytes (CTL) responses in CHB patients.Methods and Materials: To this end, the human hepatoma HepG2.2.15 cell line was used to produce HBVsvp as a culturing system, and HBVsvp were concentrated for highly virus titer using the polyethylene glycol protocol. Peripheral blood mononuclear cells (PBMCs), collected from CHB patients and healthy donors, were differentiated into MoDCs and T cells. PBMCs-derived MoDCs were first pulsed with HBVsvp and then cultured with PBMCs-derived T cells. MoDCs and/or T subsets cells were identified for phenotypic activation by FACS ana

Published
2020

16. GLUTATHIONE-S-TRANSFERASE (GSTP1) GENE POLYMORPHISM AND SUSCEPTIBILITY TO HEPATOCELLULAR CARCINOMA IN EGYPT.

Author

El Shafie, Maathir K., Shebl, Nashwa, El-Sabwaay, Malta, Abbas, Sherif, and Kamal, Samar

Subjects
*GLUTATHIONE transferase, *GENETIC polymorphisms, *DISEASE susceptibility, *LIVER cancer, *HEPATITIS C virus, *CONTROL groups
Abstract

Hepatocellular carcinoma (HCC) is one of most frequent cancer in the world, genetic polymorphisms have been reported to play a role in susceptibility to HCC. The present study aimed to study the possible association between glutathione -- S -- transferase (GSTP1) gene polymorphism and susceptibility to HCC. The study was carried out on 120 subjects divided into 3 groups : Group (A) included 60 HCC patients and group (B) included 40 chronic hepatitis C virus patients and group (C) included 20 age and sex matched healthy control. All subjects were submitted to full history taking, liver function tests, GSTP1 gene polymorphism by PCR -- RFLP. This study found a significant difference between HCC group and each of hepatitis C virus group ( HCV) and control group, while there is no significant differences between HCV group and the control group as regarding GSTP1 genotyping with the highest percent of ile/val polymorphism (IV) and val/val polymorphism (VV) in HCC group and ile/ile (II) polymorphism among HCV and the control group. GSTP1 IV genotype frequency was associated with 5.53 times higher risk of HCC than GSTP1 II genotype, while GSTP1 VV genotype frequency was associated with 6.40 times higher risk of HCC than GSTP1 II genotype when compared to the other two groups together. The frequency of GSTP1 val (V) allele is higher in HCC when compared to the other two groups together and it was associated with 2.70 times higher risk of HCC than GSTP1 I allele. The present study reported that carriage of GSTP1 ile/val and val/val genotypes have a role in susceptibility to HCC and this susceptibility are not through the alteration of the expression of clinical pathological markers and we recommend performance of this work on a large scale to confirm these results. [ABSTRACT FROM AUTHOR]

Published
2014

Catalog

Books, media, physical & digital resources
See catalog results